NF-Y CCAAT-binding factor in Drosophila melanogaster

NF-Y is a heterotrimeric transcription factor that binds the CCAAT box found in many eukaryotic promoters. Nf-YB and Nf-YC form a histone-fold dimer that binds DNA nonspecifically and creates the surface for Nf-YA, which confers sequence-specific recognition of CCAAT. In Drosophila, NF-Y regulates genes in eye and neural development and in cell proliferation.

MODULE:dmel_nf_y_complexDRAFTCONCRETEProtein Complexmodules/dmel_nf_y_complex.yaml
CCAAT-binding factor complexGO:0016602
FB:FBgg0000773
FlyBase gene group CBFTF: Ccaat-binding factor complex
FlyBase release FB2026_03 groups 3 D. melanogaster genes as ccaat-binding factor complex (CBFTF); this module models that group.
FB:FBgg0002205
FlyBase gene group NFY: Ccaat-binding factor complex subunits
FlyBase release FB2026_03 groups 3 D. melanogaster genes as ccaat-binding factor complex subunits (NFY); this module models that group.
GO:0016602
CCAAT-binding factor complex
GO term grounding the module as CCAAT-binding factor complex.

FlyBase lists the same three genes in two groups (CBFTF, the complex, and NFY, the subunit family); both are realized by this module. The trimer binds CCAAT boxes as a unit (the NF-YB-NF-YC histone-fold dimer with NF-YA), so DNA-binding transcription factor activity (GO:0000981), which all three subunits carry in GOA by IBA, is recorded on a complex-level annoton. FlyBase groups realized by this module: CBFTF (FBgg0000773), NFY (FBgg0002205). Participants were generated from the FlyBase gene-group membership and mapped to UniProtKB by projects/FLYBASE_GENE_GROUPS/generate_modules.py from module_specs/dmel_nf_y_complex.yaml. Member genes with a gene review in this repository: Nf-YA, Nf-YB, Nf-YC.

4Nodes
3Parts
0Variant Sets
0Variants
4Annotons
0Connections

Derived QC

Recommended-field compliance

100.0% recommended fields populated

All recommended fields populated.

Module deep research

✗ none found

No MODULE:dmel_nf_y_complex deep-research report alongside the module YAML.

Leaf nodes lacking representative members

✓ every leaf node grounds to a representative protein.

Template conformance

✓ every declared conforms_to bundle matches its template motif.

Function consistency and core coverage

0 gene-function check(s) reflected in core functions · 0 conflict(s) · 0 evidence or coverage gap(s) (1 comparison(s))

Retained noncore functions are reported separately; they do not require promotion to core functions. Support from representative genes does not establish conservation across the whole family. Missing reviews or identifiers leave a claim unverified.

AnnotonParticipantFunctionAssessment
dmel_nf_y_complex_complex_activity PROTEIN_COMPLEX
Gene 		DNA-binding transcription factor activity, RNA polymerase II-specific
GO:0000981 		Participant Unchecked
No direct UniProt participant to compare. Abstract selectors and complex-wide activities cannot be inferred from donor or subunit gene reviews.

Gene-review completeness (3/3 grounded genes reviewed)

3 complete review(s) · 3 with deep research · 0 missing review · 0 reviewed but lacking deep research

Gene Review Complete Deep research
Nf-YA Q9VSY9 ✓ ✓ ✓
Nf-YB Q8ST61 ✓ ✓ ✓
Nf-YC Q9W3V9 ✓ ✓ ✓

Details

Context
Drosophila melanogasterNCBITaxon:7227
nucleusGO:0005634
NF-Y complexProtein Complexdmel_nf_y_complex

Heterotrimeric Nf-YA/Nf-YB/Nf-YC transcription factor that binds CCAAT boxes.

CCAAT-binding factor complexGO:0016602
Context
Drosophila melanogasterNCBITaxon:7227
nucleusGO:0005634

Annotons

NF-Y complex activity
dmel_nf_y_complex_complex_activity
Participant: Protein Complex: NF-Y complex
Protein Complex:
NF-Y complexGO:0016602
Active units:
Nf-YA
Participant: Gene Product: Nf-YA
Gene Product:
Nf-YAUniProtKB:Q9VSY9
Role: sequence-specific CCAAT recognition subunit
Nf-YB
Participant: Gene Product: Nf-YB
Gene Product:
Nf-YBUniProtKB:Q8ST61
Role: histone-fold subunit
Nf-YC
Participant: Gene Product: Nf-YC
Gene Product:
Nf-YCUniProtKB:Q9W3V9
Role: histone-fold subunit

Function

DNA-binding transcription factor activity, RNA polymerase II-specificGO:0000981

Binds CCAAT boxes in promoters as an NF-YA-NF-YB-NF-YC trimer and regulates RNA polymerase II transcription.

Part 1: sequence-specific CCAAT recognition subunit
Nf-YAModuledmel_nf_y_complex_nf_ya

Annotons

Nf-YA subunit
dmel_nf_y_complex_nf_ya_nf_ya
Participant: Gene Product: Nf-YA
Gene Product:
Nf-YAUniProtKB:Q9VSY9

sequence-specific CCAAT recognition subunit

Part 2: histone-fold subunit
Nf-YBModuledmel_nf_y_complex_nf_yb

Annotons

Nf-YB subunit
dmel_nf_y_complex_nf_yb_nf_yb
Participant: Gene Product: Nf-YB
Gene Product:
Nf-YBUniProtKB:Q8ST61

histone-fold subunit

Part 3: histone-fold subunit
Nf-YCModuledmel_nf_y_complex_nf_yc

Annotons

Nf-YC subunit
dmel_nf_y_complex_nf_yc_nf_yc
Participant: Gene Product: Nf-YC
Gene Product:
Nf-YCUniProtKB:Q9W3V9

histone-fold subunit