Prefoldin is a heterohexameric co-chaperone shaped like a jellyfish, with six coiled-coil tentacles that bind nascent or unfolded polypeptides, notably actin and tubulin, and pass them to the CCT chaperonin. It is built from two alpha-class subunits (Pfdn3, encoded by mgr, and Pfdn5) and four beta-class subunits (Pfdn1, Pfdn2, Pfdn4 and Pfdn6). In Drosophila, mgr is needed for normal tubulin levels and spindle formation.
The alpha/beta class split follows the conserved prefoldin architecture. The former GO term for unfolded protein binding is obsolete; the ATP-independent protein folding chaperone term (GO:0044183), which Pfdn1 carries in GOA by IBA, is recorded on a complex-level annoton with all six subunits as active units, matching the placement in modules/cct_chaperonin_folding.yaml. FlyBase groups realized by this module: PFDN (FBgg0001640). Participants were generated from the FlyBase gene-group membership and mapped to UniProtKB by projects/FLYBASE_GENE_GROUPS/generate_modules.py from module_specs/dmel_prefoldin.yaml. None of the member genes has a gene review in this repository yet.
All recommended fields populated.
✗ none found
No MODULE:dmel_prefoldin deep-research report alongside the module YAML.
✓ every leaf node grounds to a representative protein.
✓ every declared conforms_to bundle matches its template motif.
0 gene-function check(s) reflected in core functions · 0 conflict(s) · 0 evidence or coverage gap(s) (1 comparison(s))
Retained noncore functions are reported separately; they do not require promotion to core functions. Support from representative genes does not establish conservation across the whole family. Missing reviews or identifiers leave a claim unverified.
| Annoton | Participant | Function | Assessment |
|---|---|---|---|
| dmel_prefoldin_complex_activity | PROTEIN_COMPLEX Gene |
protein folding chaperone GO:0044183 |
Participant Unchecked
No direct UniProt participant to compare. Abstract selectors and complex-wide activities cannot be inferred from donor or subunit gene reviews. |
0 complete review(s) · 0 with deep research · 6 missing review · 0 reviewed but lacking deep research
| Gene | Review | Complete | Deep research |
|---|---|---|---|
| Pfdn5 Q9VCZ8 | ✗ | — | — |
| mgr Q9VGP6 | ✗ | — | — |
| Pfdn1 Q9VMH8 | ✗ | — | — |
| Pfdn4 Q9VRL3 | ✗ | — | — |
| Pfdn2 Q9VTE5 | ✗ | — | — |
| Pfdn6 Q9VW56 | ✗ | — | — |
Heterohexameric co-chaperone of two alpha and four beta subunits that delivers unfolded actin and tubulin to CCT.
Captures non-native actin and tubulin chains without ATP and transfers them to the CCT chaperonin.