The general transcription factors TFIID, TFIIA, TFIIB, TFIIE, TFIIF and TFIIH assemble with RNA polymerase II on core promoters to form the preinitiation complex. TFIID, built from the TATA-box binding protein (TBP) and TBP-associated factors (TAFs), recognizes core promoter elements; TFIIA stabilizes TBP binding to DNA. TFIIF escorts polymerase into the complex and TFIIE recruits TFIIH. TFIIH contains the XPB (hay) translocase that opens promoter DNA, the XPD helicase, and the CDK-activating kinase (Cdk7, CycH, Mat1), which phosphorylates the polymerase C-terminal domain during initiation; TFIIH also acts in nucleotide excision repair. In Drosophila testis, paralogous testis-specific TAFs (tTAFs) and a germline TFIIA small subunit variant drive the spermatocyte transcription program.
Boundary. TFIIB (TfIIB) has no FlyBase group of its own in this set and is not added. FlyBase's CAK-XPD group (Cdk7, CycH, Mat1, Xpd) is fully covered by the CAK part plus Xpd in the TFIIH core part, so it is not modelled as a separate node. Gas41 and caz are FlyBase TFIID group members kept as listed; they are not canonical TAFs. The tTAFs are emitted one annoton per gene because the composition of testis TFIID-like complexes is not settled. RNA polymerase II itself is in dmel_nuclear_rna_polymerases. FlyBase groups realized by this module: TFIID (FBgg0000347), TFIIE (FBgg0000348), TFIIF (FBgg0000349), TFIIH (FBgg0000350), T-TAF (FBgg0000352), CAK-XPD (FBgg0000354), TFIIA (FBgg0000355). Subgroups folded into it: CAK, TFIIH-C, TFIIA-S, TFIIA-S2. Participants were generated from the FlyBase gene-group membership and mapped to UniProtKB by projects/FLYBASE_GENE_GROUPS/generate_modules.py from module_specs/dmel_rnapii_general_transcription_factors.yaml. None of the member genes has a gene review in this repository yet.
All recommended fields populated.
✗ none found
No MODULE:dmel_rnapii_general_transcription_factors deep-research report alongside the module YAML.
✓ every leaf node grounds to a representative protein.
✓ every declared conforms_to bundle matches its template motif.
0 gene-function check(s) reflected in core functions · 0 conflict(s) · 2 evidence or coverage gap(s) (5 comparison(s))
Retained noncore functions are reported separately; they do not require promotion to core functions. Support from representative genes does not establish conservation across the whole family. Missing reviews or identifiers leave a claim unverified.
| Annoton | Participant | Function | Assessment |
|---|---|---|---|
| tfiid_complex | PROTEIN_COMPLEX Gene |
RNA polymerase II general transcription initiation factor activity GO:0016251 |
Participant Unchecked
No direct UniProt participant to compare. Abstract selectors and complex-wide activities cannot be inferred from donor or subunit gene reviews. |
| tfiie_complex | PROTEIN_COMPLEX Gene |
RNA polymerase II general transcription initiation factor activity GO:0016251 |
Participant Unchecked
No direct UniProt participant to compare. Abstract selectors and complex-wide activities cannot be inferred from donor or subunit gene reviews. |
| tfiif_complex | PROTEIN_COMPLEX Gene |
RNA polymerase II general transcription initiation factor activity GO:0016251 |
Participant Unchecked
No direct UniProt participant to compare. Abstract selectors and complex-wide activities cannot be inferred from donor or subunit gene reviews. |
| tfiih_core_complex/xpd | Xpd UniProtKB:Q7KVP9 Complex Unit |
5'-3' DNA helicase activity GO:0043139 |
Review Missing
No ai-review exists for this protein; its function and core-function coverage cannot yet be checked. |
| cak_complex/cdk7 | Cdk7 UniProtKB:Q24216 Complex Unit |
RNA polymerase II CTD heptapeptide repeat kinase activity GO:0008353 |
Review Missing
No ai-review exists for this protein; its function and core-function coverage cannot yet be checked. |
0 complete review(s) · 0 with deep research · 40 missing review · 0 reviewed but lacking deep research
| Gene | Review | Complete | Deep research |
|---|---|---|---|
| ear A0A0B4KG69 | ✗ | — | — |
| Mat1 A1Z8B6 | ✗ | — | — |
| Tfb5 B7Z018 | ✗ | — | — |
| CycH O76513 | ✗ | — | — |
| TfIIEalpha O96880 | ✗ | — | — |
| TfIIEbeta O96881 | ✗ | — | — |
| Tbp P20227 | ✗ | — | — |
| TfIIFbeta P41900 | ✗ | — | — |
| Taf4 P47825 | ✗ | — | — |
| Taf5 P49846 | ✗ | — | — |
| Taf6 P49847 | ✗ | — | — |
| Taf12 P49905 | ✗ | — | — |
| Taf11 P49906 | ✗ | — | — |
| Taf1 P51123 | ✗ | — | — |
| TfIIA-L P52654 | ✗ | — | — |
| TfIIA-S P52656 | ✗ | — | — |
| hay Q02870 | ✗ | — | — |
| TfIIFalpha Q05913 | ✗ | — | — |
| Cdk7 Q24216 | ✗ | — | — |
| Taf2 Q24325 | ✗ | — | — |
| Taf9 Q27272 | ✗ | — | — |
| caz Q27294 | ✗ | — | — |
| Xpd Q7KVP9 | ✗ | — | — |
| Tfb1 Q960E8 | ✗ | — | — |
| Taf10 Q9U5W9 | ✗ | — | — |
| Taf3 Q9V4D4 | ✗ | — | — |
| Taf7 Q9VHY5 | ✗ | — | — |
| Taf13 Q9VIP1 | ✗ | — | — |
| nht Q9VJP5 | ✗ | — | — |
| Gas41 Q9VM63 | ✗ | — | — |
| mia Q9VND0 | ✗ | — | — |
| Ssl1 Q9VNP8 | ✗ | — | — |
| sa Q9VP40 | ✗ | — | — |
| Tfb4 Q9VPX4 | ✗ | — | — |
| Taf12L Q9VR21 | ✗ | — | — |
| can Q9VT91 | ✗ | — | — |
| mrn Q9VUR1 | ✗ | — | — |
| Taf8 Q9VWY6 | ✗ | — | — |
| TfIIA-S-2 Q9W5B9 | ✗ | — | — |
| Taf10b Q9XZT7 | ✗ | — | — |
TFIID, TFIIA, TFIIE, TFIIF and TFIIH, which assemble the RNA polymerase II preinitiation complex at core promoters.
TBP and TBP-associated factors. FlyBase also lists Gas41 (YEATS domain) and cabeza (caz, FET family) in this group as TFIID-associated proteins.
TfIIFalpha and TfIIFbeta; FlyBase also includes ear, the YEATS-domain ENL/AF9 relative, whose yeast counterpart Taf14 is a TFIIF subunit.
tTAFs (can, mia, nht, sa, Taf12L) are paralogs of TFIID TAFs expressed in primary spermatocytes, where they are needed for transcription of spermatid differentiation genes and meiotic cell-cycle genes.