The KEOPS (EKC) complex transfers a threonylcarbamoyl group onto adenosine 37 of tRNAs that decode ANN codons, forming t6A, a modification needed for accurate decoding. Threonylcarbamoyl-AMP is supplied by a separate enzyme (Sua5/YRDC). The catalytic subunit is the Kae1/OSGEP ortholog (Tcs3 in flies); Bud32/TP53RK (Tcs5) is an atypical kinase-like ATPase subunit and Pcc1/LAGE3 (Tcs6) is a small dimerization subunit. In Drosophila, KEOPS subunits are required for growth.
Modelled one part per subunit. The Cgi121 (TPRKB) and Gon7 subunits found in yeast and human KEOPS are not in the FlyBase group and are not included. The mitochondrial t6A enzyme (OSGEPL1 ortholog) and YRDC are outside this module. FlyBase groups realized by this module: TCTC (FBgg0001634). Participants were generated from the FlyBase gene-group membership and mapped to UniProtKB by projects/FLYBASE_GENE_GROUPS/generate_modules.py from module_specs/dmel_threonylcarbamoyl_transferase_complex.yaml. Member genes with a gene review in this repository: Tcs3, Tcs5, Tcs6.
All recommended fields populated.
✗ none found
No MODULE:dmel_threonylcarbamoyl_transferase_complex deep-research report alongside the module YAML.
✓ every leaf node grounds to a representative protein.
✓ every declared conforms_to bundle matches its template motif.
1 gene-function check(s) reflected in core functions · 0 conflict(s) · 0 evidence or coverage gap(s) (1 comparison(s))
Retained noncore functions are reported separately; they do not require promotion to core functions. Support from representative genes does not establish conservation across the whole family. Missing reviews or identifiers leave a claim unverified.
| Annoton | Participant | Function | Assessment |
|---|---|---|---|
| tcs3_subunit_tcs3 | Tcs3 UniProtKB:Q9VV41 Gene |
tRNA N(6)-L-threonylcarbamoyladenine synthase activity GO:0061711 |
Core Supported
The GO molecular function is reflected in core_functions (exact term or a reviewed subclass). |
3 complete review(s) · 3 with deep research · 0 missing review · 0 reviewed but lacking deep research
| Gene | Review | Complete | Deep research |
|---|---|---|---|
| Tcs3 Q9VV41 | ✓ | ✓ | ✓ |
| Tcs5 Q9VRJ6 | ✓ | ✓ | ✓ |
| Tcs6 E1JIY8 | ✓ | ✓ | ✓ |
Cytoplasmic KEOPS complex that forms t6A at tRNA position 37.