Eukaryotic tRNA-guanine transglycosylase exchanges guanine 34 in the anticodon of tRNA-Asp, tRNA-Asn, tRNA-His and tRNA-Tyr for queuine, forming queuosine. Animals cannot synthesize queuine and obtain it from diet and gut microbes. The enzyme is a heterodimer of a catalytic subunit (QTRT1 ortholog, Tgt) and a catalytically inactive paralogous accessory subunit (QTRT2 ortholog, CG3434) required for activity.
Modelled one part per subunit. Subunit roles are assigned by orthology to human QTRT1 and QTRT2; direct biochemical characterization of the Drosophila enzyme is limited. FlyBase groups realized by this module: TGTC (FBgg0002008). Participants were generated from the FlyBase gene-group membership and mapped to UniProtKB by projects/FLYBASE_GENE_GROUPS/generate_modules.py from module_specs/dmel_trna_guanine_transglycosylase.yaml. Member genes with a gene review in this repository: CG3434, Tgt.
All recommended fields populated.
✗ none found
No MODULE:dmel_trna_guanine_transglycosylase deep-research report alongside the module YAML.
✓ every leaf node grounds to a representative protein.
✓ every declared conforms_to bundle matches its template motif.
1 gene-function check(s) reflected in core functions · 0 conflict(s) · 0 evidence or coverage gap(s) (1 comparison(s))
Retained noncore functions are reported separately; they do not require promotion to core functions. Support from representative genes does not establish conservation across the whole family. Missing reviews or identifiers leave a claim unverified.
| Annoton | Participant | Function | Assessment |
|---|---|---|---|
| tgt_subunit_tgt | Tgt UniProtKB:Q9VPY8 Gene |
tRNA-guanosine(34) queuine transglycosylase activity GO:0008479 |
Core Supported
The GO molecular function is reflected in core_functions (exact term or a reviewed subclass). |
2 complete review(s) · 2 with deep research · 0 missing review · 0 reviewed but lacking deep research
| Gene | Review | Complete | Deep research |
|---|---|---|---|
| CG3434 Q9VSZ6 | ✓ | ✓ | ✓ |
| Tgt Q9VPY8 | ✓ | ✓ | ✓ |
Tgt-CG3434 heterodimer that inserts salvaged queuine at tRNA position 34.