The vacuolar H+-ATPase (V-ATPase) is a rotary proton pump that acidifies lysosomes, endosomes, the Golgi and secretory vesicles, and in specialized epithelia such as the Malpighian tubules and midgut energizes the plasma membrane for transport. The cytosolic V1 domain hydrolyses ATP at three catalytic sites in an A3B3 hexamer, and the central rotor (subunits D, F and d) transmits rotation to a ring of proteolipid c subunits in the membrane V0 domain. The V0 a subunit provides the proton path together with the c ring, and peripheral stalks of E and G subunits, with C and H, hold the stator. Reversible dissociation of V1 from V0 regulates activity. In Drosophila many subunits are encoded by paralogous genes, some with tissue- or testis-restricted expression, and the accessory proteins Ac45 and ATP6AP2 associate with V0.
Decomposition. Subunits are grouped by domain and by subunit type; each gene is its own annoton because paralogs of one subunit type are alternatives rather than co-subunits, and which paralogs assemble together in which tissue is not mapped. Subunit-type assignments follow the UniProt protein names. CG5421 is placed with the accessory subunits as in FlyBase; its function is not characterized. The proton-pumping activity belongs to the assembled complex; only ATP hydrolysis is attached to the catalytic A subunits. FlyBase groups realized by this module: VHA (FBgg0000111). Subgroups folded into it: VHA-ACS, VHA-V1, VHA-V0, VHA-V1-CAT, VHA-V1-NONCAT. Participants were generated from the FlyBase gene-group membership and mapped to UniProtKB by projects/FLYBASE_GENE_GROUPS/generate_modules.py from module_specs/dmel_vacuolar_atpase.yaml. None of the member genes has a gene review in this repository yet.
All recommended fields populated.
✗ none found
No MODULE:dmel_vacuolar_atpase deep-research report alongside the module YAML.
✓ every leaf node grounds to a representative protein.
✓ every declared conforms_to bundle matches its template motif.
0 gene-function check(s) reflected in core functions · 0 conflict(s) · 3 evidence or coverage gap(s) (3 comparison(s))
Retained noncore functions are reported separately; they do not require promotion to core functions. Support from representative genes does not establish conservation across the whole family. Missing reviews or identifiers leave a claim unverified.
| Annoton | Participant | Function | Assessment |
|---|---|---|---|
| v1_subunit_a_vha68_1 | Vha68-1 UniProtKB:P48602 Gene |
ATP hydrolysis activity GO:0016887 |
Review Missing
No ai-review exists for this protein; its function and core-function coverage cannot yet be checked. |
| v1_subunit_a_vha68_2 | Vha68-2 UniProtKB:Q27331 Gene |
ATP hydrolysis activity GO:0016887 |
Review Missing
No ai-review exists for this protein; its function and core-function coverage cannot yet be checked. |
| v1_subunit_a_vha68_3 | Vha68-3 UniProtKB:Q9VK47 Gene |
ATP hydrolysis activity GO:0016887 |
Review Missing
No ai-review exists for this protein; its function and core-function coverage cannot yet be checked. |
0 complete review(s) · 0 with deep research · 36 missing review · 0 reviewed but lacking deep research
| Gene | Review | Complete | Deep research |
|---|---|---|---|
| Vha36-2 A1Z8V7 | ✗ | — | — |
| Vha16-4 A1ZAL7 | ✗ | — | — |
| Vha100-3 A1ZBF7 | ✗ | — | — |
| Vha16-1 P23380 | ✗ | — | — |
| Vha55 P31409 | ✗ | — | — |
| Vha68-1 P48602 | ✗ | — | — |
| Vha26 P54611 | ✗ | — | — |
| VhaAC45RP Q0KHY8 | ✗ | — | — |
| Vha14-1 Q24583 | ✗ | — | — |
| Vha68-2 Q27331 | ✗ | — | — |
| VhaAC45 Q7JR49 | ✗ | — | — |
| Vha16-3 Q8IQG3 | ✗ | — | — |
| Vha36-3 Q9NEF6 | ✗ | — | — |
| VhaSFD Q9V3J1 | ✗ | — | — |
| Vha36-1 Q9V7D2 | ✗ | — | — |
| Vha44 Q9V7N5 | ✗ | — | — |
| VhaAC39-2 Q9VCQ3 | ✗ | — | — |
| Vha100-2 Q9VE75 | ✗ | — | — |
| Vha100-4 Q9VE77 | ✗ | — | — |
| VhaM9.7-d Q9VEQ6 | ✗ | — | — |
| VhaPPA1-1 Q9VFE3 | ✗ | — | — |
| VhaPPA1-2 Q9VFE5 | ✗ | — | — |
| ATP6AP2 Q9VHG4 | ✗ | — | — |
| Vha68-3 Q9VK47 | ✗ | — | — |
| CG5421 Q9VK83 | ✗ | — | — |
| Vha100-5 Q9VKF6 | ✗ | — | — |
| Vha16-5 Q9VKQ8 | ✗ | — | — |
| Vha14-2 Q9VNL3 | ✗ | — | — |
| VhaM9.7-b Q9VP18 | ✗ | — | — |
| Vha16-2 Q9VTI6 | ✗ | — | — |
| CG15719 Q9VYH6 | ✗ | — | — |
| VhaM9.7-a Q9VZE9 | ✗ | — | — |
| VhaM9.7-c Q9VZG7 | ✗ | — | — |
| VhaAC39-1 Q9W4P5 | ✗ | — | — |
| Vha100-1 Q9XZ10 | ✗ | — | — |
| Vha13 Q9XZH6 | ✗ | — | — |
Rotary proton pump of an ATP-hydrolysing V1 domain and a proton-translocating V0 domain, with paralogous subunit genes and V0 accessory proteins.