VHL-Elongin B/C-Cul2 ubiquitin ligase in Drosophila melanogaster

The VCB complex consists of the von Hippel-Lindau tumour suppressor protein (Vhl) bound to the adaptor heterodimer Elongin B and Elongin C. Together with Cul2 and the RING protein Roc1a it forms a cullin-RING E3 ubiquitin ligase in which Vhl is the substrate receptor. Its best-known substrates are hypoxia-inducible factor alpha subunits (Sima in Drosophila) after prolyl hydroxylation in normoxia, so the ligase couples oxygen availability to HIF-alpha degradation.

MODULE:dmel_vcb_ubiquitin_ligaseDRAFTCONCRETEProtein Complexmodules/dmel_vcb_ubiquitin_ligase.yaml
VCB complexGO:0030891 Cul2-RING ubiquitin ligase complexGO:0031462
FB:FBgg0001732
FlyBase gene group VCB: Vcb complex
FlyBase release FB2026_03 groups 5 D. melanogaster genes as vcb complex (VCB); this module models that group.
GO:0030891
VCB complex
GO term grounding the module as VCB complex.
GO:0031462
Cul2-RING ubiquitin ligase complex
GO term grounding the module as Cul2-RING ubiquitin ligase complex.

Strictly the VCB complex is Vhl-EloB-EloC; FlyBase includes Cul2 and Roc1a, so the module is the full CRL2-Vhl ligase. EloB and EloC are also subunits of the Elongin transcription elongation complex and of other Cul2/Cul5 ligases. FlyBase groups realized by this module: VCB (FBgg0001732). Participants were generated from the FlyBase gene-group membership and mapped to UniProtKB by projects/FLYBASE_GENE_GROUPS/generate_modules.py from module_specs/dmel_vcb_ubiquitin_ligase.yaml. Member genes with a gene review in this repository: Cul2, EloB, EloC, Roc1a, Vhl.

4Nodes
3Parts
0Variant Sets
0Variants
3Annotons
1Connections

Derived QC

Recommended-field compliance

100.0% recommended fields populated

All recommended fields populated.

Module deep research

✗ none found

No MODULE:dmel_vcb_ubiquitin_ligase deep-research report alongside the module YAML.

Leaf nodes lacking representative members

✓ every leaf node grounds to a representative protein.

Template conformance

✓ every declared conforms_to bundle matches its template motif.

Function consistency and core coverage

0 gene-function check(s) reflected in core functions · 0 conflict(s) · 0 evidence or coverage gap(s) (1 comparison(s))

Retained noncore functions are reported separately; they do not require promotion to core functions. Support from representative genes does not establish conservation across the whole family. Missing reviews or identifiers leave a claim unverified.

AnnotonParticipantFunctionAssessment
cul2_roc1a_core_complex PROTEIN_COMPLEX
Gene 		ubiquitin protein ligase activity
GO:0061630 		Participant Unchecked
No direct UniProt participant to compare. Abstract selectors and complex-wide activities cannot be inferred from donor or subunit gene reviews.

Gene-review completeness (5/5 grounded genes reviewed)

5 complete review(s) · 5 with deep research · 0 missing review · 0 reviewed but lacking deep research

Gene Review Complete Deep research
Cul2 Q9V9R2 ✓ ✓ ✓
EloB Q7KSB2 ✓ ✓ ✓
EloC E2QCI6 ✓ ✓ ✓
Roc1a Q9W5E1 ✓ ✓ ✓
Vhl Q9V3C1 ✓ ✓ ✓

Details

Context
Drosophila melanogasterNCBITaxon:7227
VCB-Cul2 ubiquitin ligaseProtein Complexdmel_vcb_ubiquitin_ligase

Cul2-Roc1a cullin-RING ligase with the EloB-EloC adaptor and the substrate receptor Vhl.

VCB complexGO:0030891 Cul2-RING ubiquitin ligase complexGO:0031462
Context
Drosophila melanogasterNCBITaxon:7227

Connections

vhl_receptor -> cul2_roc1a_core Provides Input For
Vhl delivers hydroxylated substrates to the catalytic core via Elongin B/C.
Part 1: cullin-RING catalytic core
Cul2-Roc1a catalytic coreProtein Complexcul2_roc1a_core

Annotons

Cul2-Roc1a catalytic core
cul2_roc1a_core_complex
Participant: Protein Complex: Cul2-Roc1a catalytic core
Protein Complex:
Cul2-Roc1a catalytic core
Active units:
Cul2
Participant: Gene Product: Cul2
Gene Product:
Cul2UniProtKB:Q9V9R2
Roc1a
Participant: Gene Product: Roc1a
Gene Product:
Roc1aUniProtKB:Q9W5E1

Function

ubiquitin protein ligase activityGO:0061630
Part 2: BC-box adaptor
Elongin B-Elongin C adaptorProtein Complexelongin_bc

Annotons

Elongin B-Elongin C adaptor
elongin_bc_complex
Participant: Protein Complex: Elongin B-Elongin C adaptor
Protein Complex:
Elongin B-Elongin C adaptor
Active units:
EloB
Participant: Gene Product: EloB
Gene Product:
EloBUniProtKB:Q7KSB2
EloC
Participant: Gene Product: EloC
Gene Product:
EloCUniProtKB:E2QCI6
Part 3: substrate receptor
Vhl substrate receptorProtein Complexvhl_receptor

Annotons

Vhl Vhl substrate receptor
vhl_receptor_vhl
Participant: Gene Product: Vhl
Gene Product:
VhlUniProtKB:Q9V3C1