WASH complex in Drosophila melanogaster

The WASH complex is a five-subunit regulatory complex on endosomes that activates the Arp2/3 complex to build branched actin patches on endosomal membranes. These patches help sort cargo into recycling tubules in cooperation with retromer. The subunits are WASH (wash), FAM21, CCDC53, SWIP and Strumpellin (Strump). WASH carries the C-terminal VCA domain that activates Arp2/3, and FAM21 links the complex to retromer and to capping protein. Drosophila wash mutant hemocytes are defective in lysosomal neutralization.

MODULE:dmel_wash_complexDRAFTCONCRETEProtein Complexmodules/dmel_wash_complex.yaml
WASH complexGO:0071203 Arp2/3 complex-mediated actin nucleationGO:0034314
FB:FBgg0000508
FlyBase gene group WASH-C: Wasp and scar homolog complex
FlyBase release FB2026_03 groups 5 D. melanogaster genes as wasp and scar homolog complex (WASH-C); this module models that group.
GO:0071203
WASH complex
GO term grounding the module as WASH complex.

Decomposition. No subcomplex is modelled; each subunit is a part. Only the WASH subunit carries the Arp2/3-activating VCA domain. The Arp2/3 complex and retromer are partners, not parts. No molecular function is assigned: the WASH complex activates the Arp2/3 complex through the VCA domain of Wash, but the fly subunits carry only binding terms in GOA. FlyBase groups realized by this module: WASH-C (FBgg0000508). Participants were generated from the FlyBase gene-group membership and mapped to UniProtKB by projects/FLYBASE_GENE_GROUPS/generate_modules.py from module_specs/dmel_wash_complex.yaml. Member genes with a gene review in this repository: CCDC53, FAM21, Strump, SWIP, wash.

6Nodes
5Parts
0Variant Sets
0Variants
5Annotons
0Connections

Derived QC

Recommended-field compliance

100.0% recommended fields populated

All recommended fields populated.

Module deep research

✗ none found

No MODULE:dmel_wash_complex deep-research report alongside the module YAML.

Leaf nodes lacking representative members

✓ every leaf node grounds to a representative protein.

Template conformance

✓ every declared conforms_to bundle matches its template motif.

Function consistency and core coverage

No functional claims were checked. This does not establish consistency with gene or family reviews.

Gene-review completeness (5/5 grounded genes reviewed)

5 complete review(s) · 5 with deep research · 0 missing review · 0 reviewed but lacking deep research

Gene Review Complete Deep research
CCDC53 Q9VLT8 ✓ ✓ ✓
FAM21 A1ZBW7 ✓ ✓ ✓
Strump Q9VUY8 ✓ ✓ ✓
SWIP Q9VXH9 ✓ ✓ ✓
wash Q7JW27 ✓ ✓ ✓

Details

Context
Drosophila melanogasterNCBITaxon:7227
endosomeGO:0005768
WASH complexProtein Complexdmel_wash_complex

Five-subunit endosomal complex in which WASH activates Arp2/3 to form actin patches for cargo recycling.

WASH complexGO:0071203 Arp2/3 complex-mediated actin nucleationGO:0034314
Context
Drosophila melanogasterNCBITaxon:7227
endosomeGO:0005768
Part 1: CCDC53 subunit
CCDC53Moduledmel_wash_complex_ccdc53

Annotons

CCDC53 subunit
dmel_wash_complex_ccdc53_ccdc53
Participant: Gene Product: CCDC53
Gene Product:
CCDC53UniProtKB:Q9VLT8

CCDC53 subunit

Part 2: FAM21 subunit, retromer and capping protein interaction
FAM21Moduledmel_wash_complex_fam21

Annotons

FAM21 subunit
dmel_wash_complex_fam21_fam21
Participant: Gene Product: FAM21
Gene Product:
FAM21UniProtKB:A1ZBW7

FAM21 subunit, retromer and capping protein interaction

Part 3: SWIP subunit
SWIPModuledmel_wash_complex_swip

Annotons

SWIP subunit
dmel_wash_complex_swip_swip
Participant: Gene Product: SWIP
Gene Product:
SWIPUniProtKB:Q9VXH9

SWIP subunit

Part 4: Strumpellin subunit
StrumpModuledmel_wash_complex_strump

Annotons

Strump subunit
dmel_wash_complex_strump_strump
Participant: Gene Product: Strump
Gene Product:
StrumpUniProtKB:Q9VUY8

Strumpellin subunit

Part 5: WASH subunit, Arp2/3 complex activator
washModuledmel_wash_complex_wash

Annotons

wash subunit
dmel_wash_complex_wash_wash
Participant: Gene Product: wash
Gene Product:
washUniProtKB:Q7JW27

WASH subunit, Arp2/3 complex activator