In canonical Wnt signaling, secreted Wnt proteins, chiefly Wingless, bind Frizzled receptors and the LRP5/6 co-receptor Arrow. Wnt secretion requires palmitoleoylation by Porcupine and transport by Wntless. In the absence of ligand, a destruction complex of Axin, Adenomatous polyposis coli (Apc or Apc2), Casein kinase I alpha and Shaggy (GSK3) phosphorylates Armadillo (beta-catenin) and targets it for degradation. Ligand engagement recruits Dishevelled and Axin to the receptor, inhibiting the destruction complex, so that Armadillo accumulates, enters the nucleus and joins Pangolin (TCF), Legless (BCL9), Pygopus, Chip and Ssdp in a Wnt enhanceosome that activates Wingless target genes. The pathway patterns the embryonic segments, the wing, and many other tissues.
Boundary. The module combines the FlyBase Wnt-TCF pathway core, Wnt production, the Wnt enhanceosome and the two destruction complex variants. Armadillo, Pangolin, Legless and Pygopus are FlyBase core components and also enhanceosome members; they are modelled once, in the enhanceosome. Axin, Shaggy and Casein kinase I alpha are shared by both destruction complex variants. The SCF-Slimb ubiquitin ligase that degrades phosphorylated Armadillo, the corepressor Groucho and other modulators are FlyBase regulator sets and not parts. Arrow has no molecular function assigned because the GO term for Wnt coreceptor activity is obsolete. FlyBase groups realized by this module: WNT-TCF (FBgg0000889), WNT-E (FBgg0000895), DES (FBgg0001810). Subgroups folded into it: WNT-TCFC, DES-C, WNT-PRO, DES-C2. FlyBase also curates regulator sets for this pathway, which are context and not modelled as parts: WNT-TCFN (negative regulators of wnt-tcf signaling pathway, 43 genes); WNT-TCFP (positive regulators of wnt-tcf signaling pathway, 52 genes). Participants were generated from the FlyBase gene-group membership and mapped to UniProtKB by projects/FLYBASE_GENE_GROUPS/generate_modules.py from module_specs/dmel_wnt_tcf_signaling.yaml. Member genes with a gene review in this repository: sgg.
All recommended fields populated.
✗ none found
No MODULE:dmel_wnt_tcf_signaling deep-research report alongside the module YAML.
✓ every leaf node grounds to a representative protein.
✓ every declared conforms_to bundle matches its template motif.
3 gene-function check(s) reflected in core functions · 0 conflict(s) · 10 evidence or coverage gap(s) (13 comparison(s))
Retained noncore functions are reported separately; they do not require promotion to core functions. Support from representative genes does not establish conservation across the whole family. Missing reviews or identifiers leave a claim unverified.
| Annoton | Participant | Function | Assessment |
|---|---|---|---|
| wnt_production_por | por UniProtKB:Q9VWV9 Gene |
palmitoleoyltransferase activity GO:1990698 |
Review Missing
No ai-review exists for this protein; its function and core-function coverage cannot yet be checked. |
| wnt_ligands_wg | wg UniProtKB:P09615 Gene |
frizzled binding GO:0005109 |
Review Missing
No ai-review exists for this protein; its function and core-function coverage cannot yet be checked. |
| wnt_ligands_wnt2 | Wnt2 UniProtKB:P28465 Gene |
frizzled binding GO:0005109 |
Review Missing
No ai-review exists for this protein; its function and core-function coverage cannot yet be checked. |
| wnt_ligands_wnt6 | Wnt6 UniProtKB:Q9VM26 Gene |
frizzled binding GO:0005109 |
Review Missing
No ai-review exists for this protein; its function and core-function coverage cannot yet be checked. |
| wnt_ligands_wnt10 | Wnt10 UniProtKB:Q9VM25 Gene |
frizzled binding GO:0005109 |
Review Missing
No ai-review exists for this protein; its function and core-function coverage cannot yet be checked. |
| wnt_receptors_fz | fz UniProtKB:P18537 Gene |
Wnt receptor activity GO:0042813 |
Review Missing
No ai-review exists for this protein; its function and core-function coverage cannot yet be checked. |
| wnt_receptors_fz2 | fz2 UniProtKB:Q9VVX3 Gene |
Wnt receptor activity GO:0042813 |
Core Supported
The GO molecular function is reflected in core_functions (exact term or a reviewed subclass). |
| destruction_complex_apc_complex/ckialpha | CkIalpha UniProtKB:P54367 Complex Unit |
protein serine/threonine kinase activity GO:0004674 |
Review Missing
No ai-review exists for this protein; its function and core-function coverage cannot yet be checked. |
| destruction_complex_apc_complex/sgg | sgg UniProtKB:P18431 Complex Unit |
protein serine/threonine kinase activity GO:0004674 |
Core Supported
The GO molecular function is reflected in core_functions (exact term or a reviewed subclass). |
| destruction_complex_apc2_complex/ckialpha | CkIalpha UniProtKB:P54367 Complex Unit |
protein serine/threonine kinase activity GO:0004674 |
Review Missing
No ai-review exists for this protein; its function and core-function coverage cannot yet be checked. |
| destruction_complex_apc2_complex/sgg | sgg UniProtKB:P18431 Complex Unit |
protein serine/threonine kinase activity GO:0004674 |
Core Supported
The GO molecular function is reflected in core_functions (exact term or a reviewed subclass). |
| wnt_enhanceosome_complex/arm | arm UniProtKB:P18824 Complex Unit |
transcription coactivator activity GO:0003713 |
Review Missing
No ai-review exists for this protein; its function and core-function coverage cannot yet be checked. |
| wnt_enhanceosome_complex/pan | pan UniProtKB:Q8IMA8 Complex Unit |
DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 |
Review Missing
No ai-review exists for this protein; its function and core-function coverage cannot yet be checked. |
✓ every negative regulator described as feedback has an upstream edge closing the loop.
0 complete review(s) · 2 with deep research · 19 missing review · 0 reviewed but lacking deep research
| Gene | Review | Complete | Deep research |
|---|---|---|---|
| arr A1Z9D7 | ✗ | — | — |
| fz2 Q9VVX3 | ✓ | 69/71 | ✓ |
| wg P09615 | ✗ | — | — |
| fz P18537 | ✗ | — | — |
| arm P18824 | ✗ | — | — |
| Wnt2 P28465 | ✗ | — | — |
| dsh P51140 | ✗ | — | — |
| CkIalpha P54367 | ✗ | — | — |
| Chi Q7KVG9 | ✗ | — | — |
| pan Q8IMA8 | ✗ | — | — |
| wls Q95ST2 | ✗ | — | — |
| lgs Q961D9 | ✗ | — | — |
| Axn Q9V407 | ✗ | — | — |
| pygo Q9V9W8 | ✗ | — | — |
| Apc Q9VAS9 | ✗ | — | — |
| Ssdp Q9VEB9 | ✗ | — | — |
| Wnt10 Q9VM25 | ✗ | — | — |
| Wnt6 Q9VM26 | ✗ | — | — |
| por Q9VWV9 | ✗ | — | — |
| Apc2 Q9Y1T2 | ✗ | — | — |
| sgg P18431 | ✓ | 129/131 | ✓ |
Wingless/Wnt binding to Frizzled and Arrow inhibits the Axin-APC destruction complex, stabilizing Armadillo, which activates targets with Pangolin/TCF in the Wnt enhanceosome.
Armadillo bound to Pangolin on Wnt response elements, with the adaptor Legless, Pygopus, and the Chip-Ssdp complex.