Bacterial fructose PTS uptake and entry into central carbon catabolism

A reusable two-part bacterial module in which a fructose-specific phosphoenolpyruvate-dependent phosphotransferase system imports fructose while converting it to fructose 1-phosphate, and 1-phosphofructokinase then converts fructose 1-phosphate to fructose 1,6-bisphosphate. The module stops at fructose 1,6-bisphosphate. Downstream central-carbon reactions, PTS regulatory cross-talk, transcriptional control by Cra/FruR, and pathways for other hexoses are outside the boundary.

MODULE:fructose_pts_uptake_and_catabolismDRAFTMetabolic Pathwaymodules/fructose_pts_uptake_and_catabolism.yaml
phosphoenolpyruvate-dependent sugar phosphotransferase systemGO:0009401 fructose catabolic processGO:0006001
GO:0006001
fructose catabolic process
GO:0006001 supplies the biological-process context for the two-step route.
PMID:18296519
Evidence of in vivo cross talk between the nitrogen-related and fructose-related branches of the carbohydrate phosphotransferase system of Pseudomonas putida.
Genetic analysis establishes FruA and FruB as the complete fructose-intake phosphotransferase system in Pseudomonas putida KT2440.
PMID:27933319
A Metabolic Widget Adjusts the Phosphoenolpyruvate-Dependent Fructose Influx in Pseudomonas putida.
The KT2440 study identifies the fruBKA locus, the FruB and FruA polyprotein architectures, and FruK as 1-phosphofructokinase.
PANTHER:PTN002412130
FruA TreeGrafter support node
Current PSEPK GOA uses this exact node as TreeGrafter/combined electronic support for FruA activity and pathway annotations.
PANTHER:PTN002238739
FruK TreeGrafter support node
Current PSEPK GOA uses this exact node as TreeGrafter/combined electronic support for FruK activity and localization annotations.
file:projects/P_PUTIDA/deep-research/PSEPK__fructose_pts_uptake_and_catabolism__ppu00051-deep-research-openscientist.md
OpenScientist PSEPK ppu00051 satisfiability review
The species-aware review finds the module satisfied by the KT2440 fruBKA locus and identifies the broader KEGG map as an overinclusive candidate source.
file:PSEPK/fruB/fruB-ai-review.yaml
PSEPK fruB gene review
Q88PQ5 supplies the soluble EI-HPr-EIIA(Fru) phosphorelay.
file:PSEPK/fruA/fruA-ai-review.yaml
PSEPK fruA gene review
Q88PQ3 supplies the membrane EIIB-EIIC transport-phosphorylation step.
file:PSEPK/fruK/fruK-ai-review.yaml
PSEPK fruK gene review
Q88PQ4 supplies 1-phosphofructokinase activity.
3Nodes
2Parts
0Variant Sets
0Variants
3Annotons
1Connections

Derived QC

Recommended-field compliance

100.0% recommended fields populated

All recommended fields populated.

Module deep research

✗ none found

No MODULE:fructose_pts_uptake_and_catabolism deep-research report alongside the module YAML.

Leaf nodes lacking representative members

✓ every leaf node grounds to a representative protein.

Template conformance

✓ every declared conforms_to bundle matches its template motif.

Gene-review completeness (3/6 grounded genes reviewed)

3 complete review(s) · 1 with deep research · 3 missing review · 2 reviewed but lacking deep research

Gene Review Complete Deep research
fruA Q88PQ3 ✓ ✓ ✗
fruB Q88PQ5 ✓ ✓ ✗
fruK Q88PQ4 ✓ ✓ ✓
Escherichia coli FruK P0AEW9 ✗ — —
Escherichia coli FruA P20966 ✗ — —
Xanthomonas campestris FruB P45597 ✗ — —

Details

Bacterial fructose PTS uptake and entry into central carbon catabolismMetabolic Pathwayfructose_pts_uptake_and_catabolism
phosphoenolpyruvate-dependent sugar phosphotransferase systemGO:0009401 fructose catabolic processGO:0006001

Exact UniProt exemplars delimit the family-level roles without restricting the module taxonomically. The phosphorelay may be encoded as a fused EI-HPr-EIIA(Fru) protein or as split components; both implement the same leaf role. PTNs encountered in source GOA are TreeGrafter or combined-IEA provenance only, not PAINT ancestral-state assertions. Transcriptional regulation, phosphoenolpyruvate supply, and PTS cross-talk are not required catalytic parts of this module.

Connections

The fructose PTS supplies intracellular fructose 1-phosphate to FruK.
Part 1: fructose import coupled to fructose 1-phosphate formation
Fructose-specific PTS import and phosphorylationBiological Processfructose_pts_import

Annotons

PEP-dependent fructose PTS phosphorelay
pep_to_fructose_pts_relay
Participant: Family: PEP-utilizing fructose PTS relay proteins
Family:
PEP-utilizing fructose PTS relay proteinsPANTHER:PTHR46244
Representative Members: PSEPK FruBUniProtKB:Q88PQ5 Xanthomonas campestris FruBUniProtKB:P45597

Function

phosphoenolpyruvate-protein phosphotransferase activityGO:0008965
Substrates: phosphoenolpyruvate L-histidyl protein
Products: pyruvate N(pros)-phospho-L-histidyl protein

Processes

phosphoenolpyruvate-dependent sugar phosphotransferase systemGO:0009401

Locations

cytoplasmGO:0005737

Introduces phosphate from PEP into the fructose-specific phosphorelay. Different bacteria may fuse or split the relay domains; Q88PQ5 and P45597 exemplify the fused architecture.

Fructose-specific EIIB-EIIC transport and phosphorylation
fructose_eiibc_transport
Participant: Family: fructose-specific PTS EIIB-EIIC proteins
Family:
fructose-specific PTS EIIB-EIIC proteinsPANTHER:PTHR30505
Representative Members: PSEPK FruAUniProtKB:Q88PQ3 Escherichia coli FruAUniProtKB:P20966

Function

protein-N(PI)-phosphohistidine-fructose phosphotransferase system transporter activityGO:0022877
Substrates: D-fructose (outside) N(pros)-phospho-L-histidyl protein
Products: beta-D-fructose 1-phosphate (inside) L-histidyl protein

Processes

fructose transmembrane transportGO:0015755 phosphoenolpyruvate-dependent sugar phosphotransferase systemGO:0009401

Locations

plasma membraneGO:0005886

Receives phosphate through its EIIB relay and couples fructose translocation through EIIC to formation of intracellular fructose 1-phosphate.

Part 2: fructose 1-phosphate conversion to fructose 1,6-bisphosphate
FruK-dependent fructose 1-phosphate phosphorylationReactionfruk_f1p_phosphorylation

Annotons

1-phosphofructokinase
fruk_activity
Participant: Family: 1-phosphofructokinase family
Family:
1-phosphofructokinase familyPANTHER:PTHR46566
Representative Members: PSEPK FruKUniProtKB:Q88PQ4 Escherichia coli FruKUniProtKB:P0AEW9

Function

1-phosphofructokinase activityGO:0008662
Substrates: beta-D-fructose 1-phosphate ATP
Products: beta-D-fructose 1,6-bisphosphate ADP proton

Processes

fructose catabolic processGO:0006001

Locations

cytosolGO:0005829

Converts the product of PTS import into fructose 1,6-bisphosphate for downstream central carbon metabolism.