GPCR-PLC-calcium-PKC signaling pathway module

A compact Gq/11-coupled GPCR signaling module. Agonist-bound GPCR activates G alpha q/11, which stimulates phospholipase C beta to cleave PIP2 into IP3 and DAG. IP3 releases calcium from intracellular stores, calcium/calmodulin activates calcium-sensitive effectors, and DAG plus calcium activates conventional PKC isoforms such as PRKCA. The pathway is grounded in GO:0007200 and includes PAINT ancestry where local seed rows support PRKCA and calmodulin roles.

MODULE:gpcr_plc_calcium_pkc_signalingDRAFTSignaling Pathwaymodules/gpcr_plc_calcium_pkc_signaling.yaml
phospholipase C-activating G protein-coupled receptor signaling pathwayGO:0007200
GO:0007200
phospholipase C-activating G protein-coupled receptor signaling pathway
The module is grounded in the GO biological process term for PLC-activating GPCR signaling.
5Nodes
4Parts
0Variant Sets
0Variants
6Annotons
3Connections

Derived QC

Recommended-field compliance

100.0% recommended fields populated

All recommended fields populated.

Module deep research

✗ none found

No MODULE:gpcr_plc_calcium_pkc_signaling deep-research report alongside the module YAML.

Leaf nodes lacking representative members

✓ every leaf node grounds to a representative protein.

Template conformance

✓ every declared conforms_to bundle matches its template motif.

Function consistency and core coverage

1 gene-function check(s) reflected in core functions · 0 conflict(s) · 6 evidence or coverage gap(s) (7 comparison(s))

Retained noncore functions are reported separately; they do not require promotion to core functions. Support from representative genes does not establish conservation across the whole family. Missing reviews or identifiers leave a claim unverified.

AnnotonParticipantFunctionAssessment
gq_coupled_receptor ADRB2 (GPCR family exemplar)
UniProtKB:P07550
Family Representative 		G protein-coupled receptor activity
GO:0004930 		Core Supported
The GO molecular function is reflected in core_functions (exact term or a reviewed subclass).
plc_beta PLCB1
UniProtKB:Q9NQ66
Gene 		phospholipase C activity
GO:0004629 		Review Missing
No ai-review exists for this protein; its function and core-function coverage cannot yet be checked.
calmodulin_sensor CALM1
UniProtKB:P0DP23
Family Representative 		calcium ion binding
GO:0005509 		Annotation Supported
A retained annotation or curated replacement supports this function, but core_functions does not yet reflect it.
protein_kinase_c PRKCA
UniProtKB:P17252
Family Representative 		protein serine/threonine kinase activity
GO:0004674 		Review Missing
No ai-review exists for this protein; its function and core-function coverage cannot yet be checked.
gq_coupled_receptor class A Gq-coupled GPCRs
PANTHER:PTHR24248
Family 		G protein-coupled receptor activity
GO:0004930 		Family Review Missing
No supported structured family review is available for PANTHER:PTHR24248.
calmodulin_sensor calmodulin/calcium-binding regulatory proteins
PANTHER:PTHR23048
Family 		calcium ion binding
GO:0005509 		Family Review Missing
No supported structured family review is available for PANTHER:PTHR23048.
protein_kinase_c conventional protein kinase C family
PANTHER:PTHR24356
Family 		protein serine/threonine kinase activity
GO:0004674 		Family Review Missing
No supported structured family review is available for PANTHER:PTHR24356.

Gene-review completeness (4/6 grounded genes reviewed)

2 complete review(s) · 2 with deep research · 2 missing review · 2 reviewed but lacking deep research

Gene Review Complete Deep research
ADRB2 P07550 ✓ ✓ ✓
CALM1 P0DP23 ✓ 168/176 ✓
GNAQ P50148 ✓ 111/121 ✗
ITPR1 Q14643 ✓ ✓ ✗
PRKCA P17252 ✗ — —
PLCB1 Q9NQ66 ✗ — —

Details

Context
eukaryotesNCBITaxon:2759
plasma membraneGO:0005886 cytosolGO:0005829
GPCR-PLC-calcium-PKC signaling pathwaySignaling Pathwaygpcr_plc_calcium_pkc_signaling
phospholipase C-activating G protein-coupled receptor signaling pathwayGO:0007200
Context
eukaryotesNCBITaxon:2759
plasma membraneGO:0005886 cytosolGO:0005829

Connections

G alpha q-GTP activates PLC beta.
IP3 produced by PLC beta opens IP3 receptors and raises cytosolic calcium.
DAG produced by PLC beta cooperates with calcium to activate PKC.
Part 1: Gq-coupled receptor activation
Gq-coupled receptor activates G alpha q/11Regulatory Stepgq_gpcr_activation

Agonist-bound receptor activates heterotrimeric Gq/11, producing G alpha q-GTP.

Annotons

Gq-coupled GPCR
gq_coupled_receptor
Participant: Family: class A Gq-coupled GPCRs
Family:
class A Gq-coupled GPCRsPANTHER:PTHR24248
Representative Members: ADRB2 (GPCR family exemplar)UniProtKB:P07550

Function

G protein-coupled receptor activityGO:0004930

Abstract Gq-coupled receptor input; ADRB2 is only a GPCR-family orientation exemplar here.

G alpha q switch
g_alpha_q
Participant: Gene Product: GNAQ
Gene Product:
GNAQUniProtKB:P50148

GTP-bound alpha subunit that activates PLC beta.

Part 2: PIP2 hydrolysis to IP3 and DAG
PLC beta hydrolyzes PIP2Reactionplc_beta_activation

PLC beta cleaves plasma-membrane PIP2 into IP3 and DAG.

Annotons

PLC beta
plc_beta
Participant: Gene Product: PLCB1
Gene Product:
PLCB1UniProtKB:Q9NQ66

Function

phospholipase C activityGO:0004629
Substrates: phosphatidylinositol 4,5-bisphosphate
Products: inositol 1,4,5-trisphosphate diacylglycerol

Effector enzyme that creates IP3 and DAG second messengers.

Part 3: calcium release and calcium-effector activation
IP3 receptor calcium release and calmodulin sensingRegulatory Stepcalcium_mobilization

IP3 opens ER calcium-release channels; cytosolic calcium is sensed by calmodulin and calcium-dependent enzymes.

Annotons

IP3 receptor calcium channel
ip3_receptor
Participant: Gene Product: ITPR1
Gene Product:
ITPR1UniProtKB:Q14643

Calcium-release channel opened by IP3.

Calmodulin calcium sensor
calmodulin_sensor
Participant: Family: calmodulin/calcium-binding regulatory proteins
Family:
calmodulin/calcium-binding regulatory proteinsPANTHER:PTHR23048
Representative Members: CALM1UniProtKB:P0DP23

Function

calcium ion bindingGO:0005509

Calcium sensor coupling calcium release to downstream enzymes.

Part 4: PKC activation by DAG and calcium
Conventional PKC activationRegulatory Steppkc_activation

DAG and calcium recruit and activate conventional protein kinase C isoforms.

Annotons

Protein kinase C alpha
protein_kinase_c
Participant: Family: conventional protein kinase C family
Family:
conventional protein kinase C familyPANTHER:PTHR24356
Representative Members: PRKCAUniProtKB:P17252

Function

protein serine/threonine kinase activityGO:0004674

DAG/calcium-responsive kinase output of the PLC branch.