Pseudomonas pyoverdine non-proteinogenic precursor supply

A reusable Pseudomonas module for reactions that supply unusual amino-acid building blocks to the cytoplasmic pyoverdine NRPS assembly line. PvdA hydroxylates L-ornithine to produce N5-hydroxy-L-ornithine, and PvdH forms L-2,4-diaminobutyrate from L-aspartate 4-semialdehyde by PLP-dependent transamination. Hydroxyornithine acylation, NRPS assembly, ferribactin export, periplasmic maturation, secretion, ferripyoverdine uptake, and iron release are outside this module.

The module is limited to precursor-supply chemistry shared across pyoverdine-producing Pseudomonas. KT2440 proteins are exemplars, while the direct biochemical evidence for PvdA and PvdH comes primarily from Pseudomonas aeruginosa homologs. No PANTHER selector is asserted because the unreviewed KT2440 accessions are not present in the local PANTHER member index. PvdY hydroxyornithine acetylation is adjacent tailoring rather than a precursor-forming reaction and remains outside this strict module boundary. PP_2800 is outside the module unless evidence distinguishes it from the dedicated cluster enzyme PvdH.