Rho protein signal transduction pathway module

Rho-family GTPase signaling uses GEFs, GAPs, and GTP-bound Rho switches to regulate actomyosin organization, adhesion, polarity, migration, and cytokinesis.

MODULE:rho_gprotein_signalingDRAFTSignaling Pathwaymodules/rho_gprotein_signaling.yaml
Rho protein signal transductionGO:0007266
file:modules/rho_gprotein_signaling-deep-research-falcon.md
Rho protein signal transduction pathway Falcon module review
Falcon/Edison module review generated 2026-06-28 is linked as supporting evidence for module boundaries, core mechanism, variation, constraints, and failure modes; open questions in that report require manual review before promotion to curated knowledge gaps.
GO:0007266
Rho protein signal transduction
The module is grounded in the GO biological process term for Rho protein signal transduction.
PANTHER:PTN000632671
PAINT node PTN000632671 for RHOA
Local PAINT IBD node in PTHR24072 with propagated terms C:GO:0005886, F:GO:0003924, F:GO:0005525, P:GO:0007015, P:GO:0007165; PAINT date 20260529.
PANTHER:PTN001178055
PAINT node PTN001178055 for RHOA
Local PAINT IBD node in PTHR24072 with propagated terms P:GO:0016477; PAINT date 20250903.
PANTHER:PTN002784793
PAINT node PTN002784793 for RHOA
Local PAINT IBD node in PTHR24072 with propagated terms P:GO:0007266, P:GO:0043149; PAINT date 20231109.
PANTHER:PTN008393484
PAINT node PTN008393484 for RHOA
Local PAINT IBD node in PTHR24072 with propagated terms C:GO:0005829, P:GO:0032956; PAINT date 20260529.

Curated compact signaling-pathway module generated from verified UniProt exemplar accessions and local PAINT IBD rows. PTN anchors are included only when the exemplar accession appears in an IBD seed row in interpro/panther; roles without local PAINT seeds remain grounded by verified UniProt exemplar accessions. Falcon integration (2026-06-28): Falcon findings are linked as evidence and family-level support. Automatically extracted open-question headings were intentionally not retained as knowledge_gap entries because they were not manually curated. Machine-generated Falcon citations remain curation support and are not manually adjudicated here.

4Nodes
3Parts
0Variant Sets
0Variants
6Annotons
2Connections

Derived QC

Recommended-field compliance

100.0% recommended fields populated

All recommended fields populated.

Module deep research

✓ present

  • rho_gprotein_signaling-deep-research-falcon.md (falcon)

Leaf nodes lacking representative members

✓ every leaf node grounds to a representative protein.

Template conformance

✓ every declared conforms_to bundle matches its template motif.

Gene-review completeness (0/4 grounded genes reviewed)

0 complete review(s) · 0 with deep research · 4 missing review · 0 reviewed but lacking deep research

Gene Review Complete Deep research
RHOA P61586 ✗ — —
ARHGAP1 Q07960 ✗ — —
ROCK1 Q13464 ✗ — —
ARHGEF1 Q92888 ✗ — —

Details

Context
metazoaNCBITaxon:33208
plasma membraneGO:0005886 cytosolGO:0005829 nucleusGO:0005634
Rho protein signal transduction pathwaySignaling Pathwayrho_gprotein_signaling
Rho protein signal transductionGO:0007266
Context
metazoaNCBITaxon:33208
plasma membraneGO:0005886 cytosolGO:0005829 nucleusGO:0005634

Connections

The initiating event promotes rho gap reset.
Rho GAP reset leads to rho kinase cytoskeletal output.
Part 1: rho nucleotide exchange
Rho nucleotide exchangeRegulatory Steprho_nucleotide_exchange

ARHGEF1 exemplifies GEF-mediated activation of Rho-family GTPases.

Annotons

Rho guanine nucleotide exchange factor
arhgef1_rho_guanine_nucleotide_exchange_factor
Participant: Gene Product: ARHGEF1
Gene Product:
ARHGEF1UniProtKB:Q92888

Curated exemplar for the rho guanine nucleotide exchange factor role in Rho protein signal transduction.

RhoA exchange substrate switch
rhoa_exchange_substrate_switch
Participant: Family: RhoA exchange substrate switch family/ortholog group
Family:
RhoA exchange substrate switch family/ortholog groupPANTHER:PTHR24072
Representative Members: RHOAUniProtKB:P61586

Curated exemplar for the RhoA exchange substrate switch role in Rho protein signal transduction.

Part 2: rho gap reset
Rho GAP resetRegulatory Steprho_gap_reset

ARHGAP1 exemplifies GAP-mediated termination of Rho-family GTPase signaling.

Annotons

Rho GTPase-activating protein
arhgap1_rho_gtpase_activating_protein
Participant: Gene Product: ARHGAP1
Gene Product:
ARHGAP1UniProtKB:Q07960

Curated exemplar for the rho gtpase-activating protein role in Rho protein signal transduction.

RhoA GAP substrate switch
rhoa_gap_substrate_switch
Participant: Family: RhoA GAP substrate switch family/ortholog group
Family:
RhoA GAP substrate switch family/ortholog groupPANTHER:PTHR24072
Representative Members: RHOAUniProtKB:P61586

Curated exemplar for the RhoA GAP substrate switch role in Rho protein signal transduction.

Part 3: rho kinase cytoskeletal output
ROCK cytoskeletal outputRegulatory Steprock_cytoskeletal_output

Activated RHOA engages ROCK kinases to regulate actomyosin and contractile responses.

Annotons

RhoA ROCK-activating switch
rhoa_rock_activating_switch
Participant: Family: RhoA ROCK-activating switch family/ortholog group
Family:
RhoA ROCK-activating switch family/ortholog groupPANTHER:PTHR24072
Representative Members: RHOAUniProtKB:P61586

Curated exemplar for the RhoA ROCK-activating switch role in Rho protein signal transduction.

ROCK1 effector kinase
rock1_rock1_effector_kinase
Participant: Gene Product: ROCK1
Gene Product:
ROCK1UniProtKB:Q13464

Curated exemplar for the rock1 effector kinase role in Rho protein signal transduction.