RidA reactive enamine/imine detoxification

A cytosolic metabolic damage-control module in which RidA accelerates hydrolytic deamination of reactive enamine/imine intermediates generated by PLP-dependent enzymes such as threonine dehydratase, protecting isoleucine biosynthesis and other PLP enzyme pathways from covalent damage.

MODULE:rida_reactive_enamine_detoxificationDRAFTCONCRETEMetabolic Pathwaymodules/rida_reactive_enamine_detoxification.yaml
2-iminobutanoate/2-iminopropanoate deaminase activityGO:0120241 threonine deaminase activityGO:0004794 L-isoleucine biosynthetic processGO:1901705
GO:0120241
2-iminobutanoate/2-iminopropanoate deaminase activity
Defines the RidA reactive imine/enamine deaminase activity.
GO:1901705
L-isoleucine biosynthetic process
Defines the branched-chain amino-acid pathway protected from IlvA-derived 2-iminobutanoate damage by RidA.
file:genes/ECOLI/RidA/RidA-ai-review.yaml
ECOLI RidA gene review
The completed E. coli RidA review grounds the enamine/imine deaminase activity and distinguishes it from HOCl-activated moonlighting holdase activity.
PMID:22094463
Conserved YjgF protein family deaminates reactive enamine/imine intermediates of pyridoxal 5'-phosphate (PLP)-dependent enzyme reactions.
Establishes the conserved RidA/YjgF-family deaminase activity toward reactive intermediates from PLP-dependent enzymes.
PMID:23696645
Redox proteomics uncovers peroxynitrite-sensitive proteins that help Escherichia coli to overcome nitrosative stress.
Demonstrates E. coli RidA enamine/imine deaminase activity and shows Cys107-dependent redox regulation of that activity.

RidA's N-chlorination-activated holdase moonlighting activity is not part of this core enamine/imine detoxification module and is intentionally left out until modification-dependent holdases are modeled more generally.

1Nodes
0Parts
0Variant Sets
0Variants
2Annotons
1Connections

Derived QC

Recommended-field compliance

100.0% recommended fields populated

All recommended fields populated.

Module deep research

✗ none found

No MODULE:rida_reactive_enamine_detoxification deep-research report alongside the module YAML.

Leaf nodes lacking representative members

✓ every leaf node grounds to a representative protein.

Template conformance

✓ every declared conforms_to bundle matches its template motif.

Reaction chaining (advisory)

✓ every PRECEDES step chains, or its break is acknowledged via chaining_status.

  • ilva_reactive_enamine_generation → rida_enamine_imine_deaminase [NOT_CHECKED]

Function consistency and core coverage

1 gene-function check(s) reflected in core functions · 0 conflict(s) · 1 evidence or coverage gap(s) (2 comparison(s))

Retained noncore functions are reported separately; they do not require promotion to core functions. Support from representative genes does not establish conservation across the whole family. Missing reviews or identifiers leave a claim unverified.

AnnotonParticipantFunctionAssessment
ilva_reactive_enamine_generation E. coli IlvA
UniProtKB:P04968
Gene 		threonine deaminase activity
GO:0004794 		Review Missing
No ai-review exists for this protein; its function and core-function coverage cannot yet be checked.
rida_enamine_imine_deaminase E. coli RidA
UniProtKB:P0AF93
Gene 		2-iminobutanoate/2-iminopropanoate deaminase activity
GO:0120241 		Core Supported
The GO molecular function is reflected in core_functions (exact term or a reviewed subclass).

Gene-review completeness (1/2 grounded genes reviewed)

1 complete review(s) · 1 with deep research · 1 missing review · 0 reviewed but lacking deep research

Gene Review Complete Deep research
E. coli IlvA P04968 ✗ — —
RidA P0AF93 ✓ ✓ ✓

Details

Context
Escherichia coliNCBITaxon:83333
RidA reactive enamine/imine detoxificationMetabolic Pathwayrida_reactive_enamine_detoxification
2-iminobutanoate/2-iminopropanoate deaminase activityGO:0120241 threonine deaminase activityGO:0004794 L-isoleucine biosynthetic processGO:1901705
Context
Escherichia coliNCBITaxon:83333

Annotons

IlvA reactive enamine generation
ilva_reactive_enamine_generation
Participant: Gene Product: E. coli IlvA
Gene Product:
E. coli IlvAUniProtKB:P04968

Function

threonine deaminase activityGO:0004794
Substrates: L-threonine
Products: 2-iminobutanoate

Processes

L-isoleucine biosynthetic processGO:1901705

Locations

cytosolGO:0005829

PLP-dependent threonine dehydratase that generates the reactive enamine/imine intermediate detoxified by RidA.

RidA enamine/imine deaminase
rida_enamine_imine_deaminase
Participant: Gene Product: E. coli RidA
Gene Product:
E. coli RidAUniProtKB:P0AF93

Function

2-iminobutanoate/2-iminopropanoate deaminase activityGO:0120241
Substrates: 2-iminobutanoate 2-iminopropanoate water
Products: 2-oxobutanoate pyruvate ammonia

Processes

L-isoleucine biosynthetic processGO:1901705

Locations

cytosolGO:0005829

Homotrimeric RidA-family enzyme that deaminates reactive PLP-derived imine/enamine species before they damage other enzymes.

Connections

IlvA generates the reactive 2-iminobutanoate intermediate that RidA hydrolytically deaminates to protect branched-chain amino-acid biosynthesis.