Bacterial taurine uptake and desulfonation

A reusable two-part bacterial module in which the TauABC ATP-binding cassette transporter imports extracellular taurine and TauD oxidatively cleaves the imported sulfonate to aminoacetaldehyde and sulfite. The module represents acquisition of taurine as a sulfur source. Downstream aminoacetaldehyde metabolism, sulfite assimilation, sulfur-starvation regulation, and alternative organosulfonate transport or cleavage systems are outside the boundary.

MODULE:taurine_uptake_and_desulfonationDRAFTMetabolic Pathwaymodules/taurine_uptake_and_desulfonation.yaml
taurine transmembrane transportGO:0015734 taurine catabolic processGO:0019529
GO:0015734
taurine transmembrane transport
GO:0015734 provides the biological-process context for TauABC-mediated uptake.
PMID:22221834
The Fe(II)/alpha-ketoglutarate-dependent taurine dioxygenases from Pseudomonas putida and Escherichia coli are tetramers.
Biochemical, kinetic, and structural work directly establishes the Pseudomonas putida TauD reaction and tetrameric assembly.
PMID:8808933
Identification of sulfate starvation-regulated genes in Escherichia coli: a gene cluster involved in the utilization of taurine as a sulfur source.
Gene disruption and sequence analysis establish TauABC as a taurine uptake system and distinguish its binding, ATPase, and membrane units.
PMID:17203388
Functional characteristics of TauA binding protein from TauABC Escherichia coli system.
Purified E. coli TauA binds taurine with high affinity and specificity.
file:modules/taurine_uptake_and_desulfonation-deep-research-openscientist.md
OpenScientist research for bacterial taurine uptake and desulfonation
The generic review evaluates the TauABC-TauD boundary, transport-complex organization, reaction chemistry, and known alternatives.
file:projects/P_PUTIDA/deep-research/PSEPK__taurine_uptake_and_desulfonation__ppu00430-deep-research-openscientist.md
OpenScientist PSEPK ppu00430 satisfiability review
The species-aware review evaluates TauABC and TauD in Pseudomonas putida KT2440 against the broader taurine and hypotaurine metabolism map.
file:PSEPK/tauA/tauA-ai-review.yaml
PSEPK tauA gene review
Q88RA0 supplies the periplasmic taurine-binding unit.
file:PSEPK/tauB/tauB-ai-review.yaml
PSEPK tauB gene review
Q88RA1 supplies the ATP-coupling units of TauABC.
file:PSEPK/tauC/tauC-ai-review.yaml
PSEPK tauC gene review
Q88RA2 supplies the transmembrane units of TauABC.
file:PSEPK/tauD/tauD-ai-review.yaml
PSEPK tauD gene review
Q88RA3 supplies taurine dioxygenase activity.
3Nodes
2Parts
0Variant Sets
0Variants
2Annotons
1Connections

Derived QC

Recommended-field compliance

100.0% recommended fields populated

All recommended fields populated.

Module deep research

✓ present

  • taurine_uptake_and_desulfonation-deep-research-openscientist.md (openscientist)

Leaf nodes lacking representative members

✓ every leaf node grounds to a representative protein.

Template conformance

✓ every declared conforms_to bundle matches its template motif.

Gene-review completeness (4/8 grounded genes reviewed)

3 complete review(s) · 2 with deep research · 4 missing review · 2 reviewed but lacking deep research

Gene Review Complete Deep research
Escherichia coli TauD P37610 ✗ — —
Escherichia coli TauA Q47537 ✗ — —
Escherichia coli TauB Q47538 ✗ — —
Escherichia coli TauC Q47539 ✗ — —
tauA Q88RA0 ✓ ✓ ✗
tauB Q88RA1 ✓ ✓ ✗
tauC Q88RA2 ✓ 6/7 ✓
tauD Q88RA3 ✓ ✓ ✓

Details

Bacterial taurine uptake and desulfonationMetabolic Pathwaytaurine_uptake_and_desulfonation
taurine transmembrane transportGO:0015734 taurine catabolic processGO:0019529

Exact UniProt exemplars delimit the TauA, TauB, TauC, and TauD roles without restricting the module taxonomically. TauA, TauB, TauC, and TauD use the exact functional PANTHER subfamilies present in the exemplars rather than their broader parent families. TauABC stoichiometry follows reviewed bacterial transporter records. Sulfur-starvation regulation, downstream aminoacetaldehyde and sulfite metabolism, and alternative sulfonate systems remain outside the boundary. PTNs encountered in source GOA are provenance only; no ancestral node is asserted without verified PAINT IBD evidence.

Connections

tauabc_import -> taud_desulfonation Provides Input For
TauABC supplies intracellular taurine to TauD.
Part 1: taurine uptake
TauABC taurine importProtein Complextauabc_import

Annotons

TauABC taurine transporter
tauabc_transporter_activity
Participant: Protein Complex: TauABC taurine importer
Protein Complex:
TauABC taurine importer Binding-protein-dependent bacterial ABC importer composed of a periplasmic TauA substrate-binding unit, two TauC transmembrane units, and two TauB ATP-coupling units.
Active units:
TauA periplasmic taurine-binding unit1
Participant: Family: TauA taurine-binding proteins
Family:
TauA taurine-binding proteinsPANTHER:PTHR30024:SF47
Representative Members: PSEPK TauAUniProtKB:Q88RA0 Escherichia coli TauAUniProtKB:Q47537
Role: Captures taurine in the periplasm and presents it to TauC.
TauC transmembrane unit2
Participant: Family: TauC taurine permeases
Family:
TauC taurine permeasesPANTHER:PTHR30151:SF25
Representative Members: PSEPK TauCUniProtKB:Q88RA2 Escherichia coli TauCUniProtKB:Q47539
Role: Forms the membrane pathway for taurine translocation.
TauB ATP-coupling unit2
Participant: Family: TauB taurine-import ATPases
Family:
TauB taurine-import ATPasesPANTHER:PTHR42788:SF18
Representative Members: PSEPK TauBUniProtKB:Q88RA1 Escherichia coli TauBUniProtKB:Q47538
Role: Couples ATP hydrolysis to taurine import.

Function

ABC-type taurine transporter transporter activityGO:0015411
Substrates: taurine (outside) ATP water
Products: taurine (inside) ADP phosphate proton

Processes

taurine transmembrane transportGO:0015734

Locations

plasma membraneGO:0005886

Imports taurine for intracellular desulfonation.

Part 2: taurine desulfonation
TauD taurine dioxygenationReactiontaud_desulfonation

Annotons

Taurine dioxygenase
taud_activity
Participant: Family: alpha-ketoglutarate-dependent sulfonate dioxygenases
Family:
alpha-ketoglutarate-dependent sulfonate dioxygenasesPANTHER:PTHR30468:SF1
Representative Members: PSEPK TauDUniProtKB:Q88RA3 Escherichia coli TauDUniProtKB:P37610
Required Function:
taurine dioxygenase activityGO:0000908

Function

taurine dioxygenase activityGO:0000908
Substrates: taurine 2-oxoglutarate oxygen
Products: aminoacetaldehyde sulfite succinate carbon dioxide proton
Cofactors: iron(2+)

Processes

taurine catabolic processGO:0019529

Locations

cytoplasmGO:0005737

Couples 2-oxoglutarate decarboxylation to oxidative cleavage of taurine, releasing sulfite and aminoacetaldehyde.