Bacterial taurine uptake and desulfonation

A reusable two-part bacterial module in which the TauABC ATP-binding cassette transporter imports extracellular taurine and TauD oxidatively cleaves the imported sulfonate to aminoacetaldehyde and sulfite. The module represents acquisition of taurine as a sulfur source. Downstream aminoacetaldehyde metabolism, sulfite assimilation, sulfur-starvation regulation, and alternative organosulfonate transport or cleavage systems are outside the boundary.

MODULE:taurine_uptake_and_desulfonationCOMPLETEABSTRACTMetabolic Pathwaymodules/taurine_uptake_and_desulfonation.yaml
taurine transmembrane transportGO:0015734 taurine catabolic processGO:0019529
GO:0015734
taurine transmembrane transport
GO:0015734 provides the biological-process context for TauABC-mediated uptake.
PMID:22221834
The Fe(II)/alpha-ketoglutarate-dependent taurine dioxygenases from Pseudomonas putida and Escherichia coli are tetramers.
Biochemical, kinetic, and structural work directly establishes the Pseudomonas putida TauD reaction and tetrameric assembly.
PMID:8808933
Identification of sulfate starvation-regulated genes in Escherichia coli: a gene cluster involved in the utilization of taurine as a sulfur source.
Gene disruption and sequence analysis establish TauABC as a taurine uptake system and distinguish its binding, ATPase, and membrane units.
PMID:17203388
Functional characteristics of TauA binding protein from TauABC Escherichia coli system.
Purified E. coli TauA binds taurine with high affinity and specificity.
file:modules/taurine_uptake_and_desulfonation-deep-research-openscientist.md
OpenScientist research for bacterial taurine uptake and desulfonation
The generic review evaluates the TauABC-TauD boundary, transport-complex organization, reaction chemistry, and known alternatives.
file:projects/P_PUTIDA/deep-research/PSEPK__taurine_uptake_and_desulfonation__ppu00430-deep-research-openscientist.md
OpenScientist PSEPK ppu00430 satisfiability review
The species-aware review evaluates TauABC and TauD in Pseudomonas putida KT2440 against the broader taurine and hypotaurine metabolism map.
file:PSEPK/tauA/tauA-ai-review.yaml
PSEPK tauA gene review
Q88RA0 supplies the periplasmic taurine-binding unit.
file:PSEPK/tauB/tauB-ai-review.yaml
PSEPK tauB gene review
Q88RA1 supplies the ATP-coupling units of TauABC.
file:PSEPK/tauC/tauC-ai-review.yaml
PSEPK tauC gene review
Q88RA2 supplies the transmembrane units of TauABC.
file:PSEPK/tauD/tauD-ai-review.yaml
PSEPK tauD gene review
Q88RA3 supplies taurine dioxygenase activity.
PMID:10781534
Deletion analysis of the Escherichia coli taurine and alkanesulfonate transport systems.
In-frame deletions with complementation show that taurine enters E. coli only through TauABC, that TauABC also imports several other sulfonates, and that Tau and Ssu components cannot form functional hybrid transporters.
PMID:9287300
Characterization of alpha-ketoglutarate-dependent taurine dioxygenase from Escherichia coli.
Purified E. coli TauD converts taurine to sulfite and aminoacetaldehyde, consuming O2 and 2-oxoglutarate, with an absolute Fe(II) requirement.
file:ECOLI/tauA/tauA-ai-review.yaml
ECOLI tauA gene review
Q47537 is the experimentally characterized periplasmic taurine-binding unit.
file:ECOLI/tauB/tauB-ai-review.yaml
ECOLI tauB gene review
Q47538 is the ATP-coupling unit of the reference TauABC importer.
file:ECOLI/tauC/tauC-ai-review.yaml
ECOLI tauC gene review
Q47539 is the inner-membrane permease unit of the reference TauABC importer.
file:ECOLI/tauD/tauD-ai-review.yaml
ECOLI tauD gene review
P37610 is the biochemically characterized taurine dioxygenase.
file:VARPD/tauA/tauA-ai-review.yaml
Variovorax paradoxus tauA gene review
A0A0H2M727 (VPARA_03650) supplies the periplasmic taurine-binding subunit TauA of the TauABC importer in Variovorax paradoxus TBEA6.
Periplasmic taurine-binding subunit of the TauAB1C ABC importer complex that captures extracellular taurine with high affinity in the periplasm and delivers it to the TauC transmembrane permease for ATP-driven import into the cytoplasm.
file:VARPD/tauB1/tauB1-ai-review.yaml
Variovorax paradoxus tauB1 gene review
A0A0H2MNF8 (VPARA_03660) supplies the ATP-binding and hydrolysis subunit TauB1 of the TauABC taurine importer in Variovorax paradoxus TBEA6.
ATP-binding and ATP-hydrolyzing catalytic subunit of the TauAB1C ABC importer complex that couples ATP hydrolysis at the cytoplasmic face of the inner membrane to transmembrane import of taurine.
file:VARPD/tauC/tauC-ai-review.yaml
Variovorax paradoxus tauC gene review
A0A0H2M7Y1 (VPARA_03670) supplies the integral inner-membrane permease subunit TauC of the TauABC taurine importer in Variovorax paradoxus TBEA6.
Integral inner-membrane transmembrane permease subunit of the TauAB1C ABC importer complex that forms the membrane translocation pathway for importing taurine from the periplasmic binding protein TauA into the cytoplasm coupled to ATP hydrolysis by TauB1.
file:VARPD/tauD1/tauD1-ai-review.yaml
Variovorax paradoxus tauD1 gene review
A0A0H2MHV8 (VPARA_23370) supplies Fe(II)/2-oxoglutarate-dependent taurine dioxygenase activity in Variovorax paradoxus TBEA6.
Fe(II)- and 2-oxoglutarate-dependent oxygenolytic desulfonation of taurine to sulfite and aminoacetaldehyde in the cytosol for sulfur assimilation.
file:interpro/panther/PTHR30024/PTHR30024-review.yaml
PTHR30024 family review
TauA proteins form SF47 of the solute-binding protein family.
file:interpro/panther/PTHR30151/PTHR30151-review.yaml
PTHR30151 family review
TauC permeases form SF25; taurine transport is scoped to that subfamily.
file:interpro/panther/PTHR42788/PTHR42788-review.yaml
PTHR42788 family review
TauB ATPases form SF18; ABC-type taurine transporter activity is scoped to that subfamily.
file:interpro/panther/PTHR30468/PTHR30468-review.yaml
PTHR30468 family review
Taurine dioxygenase activity belongs to the bacterial TauD clade (PTN000765929) inside the mixed SF1, which also holds RdpA, JLP1 and fungal cluster dioxygenases.
3Nodes
2Parts
0Variant Sets
0Variants
2Annotons
1Connections

Derived QC

Recommended-field compliance

100.0% recommended fields populated

All recommended fields populated.

Module deep research

✓ present

  • taurine_uptake_and_desulfonation-deep-research-openscientist.md (openscientist)

Leaf nodes lacking representative members

✓ representative grounding skipped for abstract module.

Template conformance

✓ every declared conforms_to bundle matches its template motif.

Function consistency and core coverage

3 gene-function check(s) reflected in core functions · 0 conflict(s) · 0 evidence or coverage gap(s) (5 comparison(s))

Retained noncore functions are reported separately; they do not require promotion to core functions. Support from representative genes does not establish conservation across the whole family. Missing reviews or identifiers leave a claim unverified.

AnnotonParticipantFunctionAssessment
tauabc_transporter_activity PROTEIN_COMPLEX
Gene 		ABC-type taurine transporter transporter activity
GO:0015411 		Participant Unchecked
No direct UniProt participant to compare. Abstract selectors and complex-wide activities cannot be inferred from donor or subunit gene reviews.
taud_activity tauD (Pseudomonas putida KT2440)
UniProtKB:Q88RA3
Family Representative 		taurine dioxygenase activity
GO:0000908 		Core Supported
The GO molecular function is reflected in core_functions (exact term or a reviewed subclass).
taud_activity tauD (Escherichia coli K-12)
UniProtKB:P37610
Family Representative 		taurine dioxygenase activity
GO:0000908 		Core Supported
The GO molecular function is reflected in core_functions (exact term or a reviewed subclass).
taud_activity tauD1 (Variovorax paradoxus)
UniProtKB:A0A0H2MHV8
Family Representative 		taurine dioxygenase activity
GO:0000908 		Core Supported
The GO molecular function is reflected in core_functions (exact term or a reviewed subclass).
taud_activity alpha-ketoglutarate-dependent sulfonate dioxygenases
PANTHER:PTHR30468:SF1
Family 		taurine dioxygenase activity
GO:0000908 		Family Supported
Family review supports GO:0000908 for PANTHER:PTHR30468:SF1. Taurine dioxygenase activity belongs to the bacterial TauD clade (PAINT node PTN000765929) inside SF1, represented by E. coli and P. putida TauD. The other SF1 members are listed as exceptions: the two RdpA enzymes cleave phenoxypropionate ethers, yeast JLP1 uses taurine poorly, and cnsP and cnsM are communesin-cluster enzymes. SF5, SF10 and SF31 do not carry the activity.

Gene-review completeness (12/12 grounded genes reviewed)

12 complete review(s) · 2 with deep research · 0 missing review · 10 reviewed but lacking deep research

Gene Review Complete Deep research
tauA A0A0H2M727 ✓ ✓ ✗
tauA Q47537 ✓ ✓ ✗
tauA Q88RA0 ✓ ✓ ✗
tauB Q47538 ✓ ✓ ✗
tauB Q88RA1 ✓ ✓ ✗
tauB1 A0A0H2MNF8 ✓ ✓ ✗
tauC A0A0H2M7Y1 ✓ ✓ ✗
tauC Q47539 ✓ ✓ ✗
tauC Q88RA2 ✓ ✓ ✓
tauD P37610 ✓ ✓ ✗
tauD Q88RA3 ✓ ✓ ✓
tauD1 A0A0H2MHV8 ✓ ✓ ✗

Details

Bacterial taurine uptake and desulfonationMetabolic Pathwaytaurine_uptake_and_desulfonation
taurine transmembrane transportGO:0015734 taurine catabolic processGO:0019529

The module is species-neutral (ABSTRACT): each role is a PANTHER subfamily with exemplars from E. coli K-12 (the experimental reference implementation), P. putida KT2440, and Variovorax paradoxus TBEA6. Every exemplar has a complete gene review. TauA, TauB and TauC use the functional PANTHER subfamilies of their exemplars (SF47, SF18, SF25) rather than the broader parent families, whose other subfamilies carry SsuABC, nitrate, bicarbonate and osmoprotectant importers. PTHR30468:SF1 is mixed: it also holds RdpA phenoxypropionate dioxygenases, yeast JLP1 and fungal cluster enzymes. The TauD annoton therefore pairs SF1 with a required taurine dioxygenase function and with the bacterial PAINT node PTN000765929, which those other members do not inherit. TauABC stoichiometry (two TauB, two TauC, one TauA) follows the curated E. coli TauB record. E. coli TauABC also imports some other short sulfonates that SsuABC carries; the module models only taurine. Sulfur-starvation regulation, downstream aminoacetaldehyde and sulfite metabolism, and alternative sulfonate systems remain outside the boundary.

Connections

tauabc_import -> taud_desulfonation Provides Input For
TauABC supplies intracellular taurine to TauD.
Part 1: taurine uptake
TauABC taurine importProtein Complextauabc_import

Annotons

TauABC taurine transporter
tauabc_transporter_activity
Participant: Protein Complex: TauABC taurine importer
Protein Complex:
TauABC taurine importer Binding-protein-dependent bacterial ABC importer composed of a periplasmic TauA substrate-binding unit, two TauC transmembrane units, and two TauB ATP-coupling units.
Active units:
TauA periplasmic taurine-binding unit1
Participant: Family: TauA taurine-binding proteins
Family:
TauA taurine-binding proteinsPANTHER:PTHR30024:SF47
Representative Members: tauA (Pseudomonas putida KT2440)UniProtKB:Q88RA0 tauA (Escherichia coli K-12)UniProtKB:Q47537 tauA (Variovorax paradoxus)UniProtKB:A0A0H2M727
Role: Captures taurine in the periplasm and presents it to TauC.
TauC transmembrane unit2
Participant: Family: TauC taurine permeases
Family:
TauC taurine permeasesPANTHER:PTHR30151:SF25
Representative Members: tauC (Pseudomonas putida KT2440)UniProtKB:Q88RA2 tauC (Escherichia coli K-12)UniProtKB:Q47539 tauC (Variovorax paradoxus)UniProtKB:A0A0H2M7Y1
Role: Forms the membrane pathway for taurine translocation.
TauB ATP-coupling unit2
Participant: Family: TauB taurine-import ATPases
Family:
TauB taurine-import ATPasesPANTHER:PTHR42788:SF18
Representative Members: tauB (Pseudomonas putida KT2440)UniProtKB:Q88RA1 tauB (Escherichia coli K-12)UniProtKB:Q47538 tauB1 (Variovorax paradoxus)UniProtKB:A0A0H2MNF8
Role: Couples ATP hydrolysis to taurine import.

Function

ABC-type taurine transporter transporter activityGO:0015411
Substrates: taurine (outside) ATP water
Products: taurine (inside) ADP phosphate proton

Processes

taurine transmembrane transportGO:0015734

Locations

plasma membraneGO:0005886

Imports taurine for intracellular desulfonation.

Part 2: taurine desulfonation
TauD taurine dioxygenationReactiontaud_desulfonation

Annotons

Taurine dioxygenase
taud_activity
Participant: Family: alpha-ketoglutarate-dependent sulfonate dioxygenases
Family:
alpha-ketoglutarate-dependent sulfonate dioxygenasesPANTHER:PTHR30468:SF1
Representative Members: tauD (Pseudomonas putida KT2440)UniProtKB:Q88RA3 tauD (Escherichia coli K-12)UniProtKB:P37610 tauD1 (Variovorax paradoxus)UniProtKB:A0A0H2MHV8
Required Function:
taurine dioxygenase activityGO:0000908

Function

taurine dioxygenase activityGO:0000908
Substrates: taurine 2-oxoglutarate oxygen
Products: aminoacetaldehyde sulfite succinate carbon dioxide proton
Cofactors: iron(2+)

Processes

taurine catabolic processGO:0019529

Locations

cytoplasmGO:0005737

Couples 2-oxoglutarate decarboxylation to oxidative cleavage of taurine, releasing sulfite and aminoacetaldehyde.