TreY/TreZ trehalose biosynthesis from alpha-glucan

A reusable two-reaction pathway in which TreY converts the reducing end of an alpha-1,4-glucan to a trehalose-containing alpha,alpha-1,1 linkage and TreZ hydrolyzes the resulting maltooligosyltrehalose to release trehalose. Alpha-glucan synthesis and remodeling, alternative OtsA/OtsB or TreS trehalose routes, and trehalose degradation are outside the boundary.

MODULE:trey_trez_trehalose_biosynthesisDRAFTMetabolic Pathwaymodules/trey_trez_trehalose_biosynthesis.yaml
trehalose biosynthetic processGO:0005992
GO:0005992
trehalose biosynthetic process
GO:0005992 provides the biological-process context.
EC:5.4.99.15
maltooligosyl trehalose synthase reaction
EC 5.4.99.15 defines the TreY glucosylmutase reaction that forms the trehalose-containing alpha-glucan terminus.
EC:3.2.1.141
malto-oligosyltrehalose trehalohydrolase reaction
EC 3.2.1.141 defines TreZ hydrolysis that releases trehalose from maltooligosyltrehalose.
PMID:8605217
Cloning and sequencing of trehalose biosynthesis genes from Arthrobacter sp. Q36
Sequence analysis identified adjacent Arthrobacter sp. Q36 treY and treZ open reading frames encoding maltooligosyl trehalose synthase and maltooligosyltrehalose trehalohydrolase, respectively.
PMID:8534970
Formation of trehalose from maltooligosaccharides by a novel enzymatic system
This historical report introduced the enzymatic system, but the cached publication record lacks an informative abstract. Mechanistic support therefore comes from PMID:8605217, PMID:8611744, and PMID:8611745.
PMID:8611744
Purification and properties of a novel enzyme, maltooligosyl trehalose synthase, from Arthrobacter sp. Q36.
Purified Arthrobacter sp. Q36 TreY converted maltooligosaccharides to maltooligosyl trehalose by intramolecular transglycosylation.
PMID:8611745
Purification and characterization of a novel enzyme, maltooligosyl trehalose trehalohydrolase, from Arthrobacter sp. Q36.
Purified Arthrobacter sp. Q36 TreZ specifically hydrolyzed the linkage between the maltooligosyl and trehalose moieties of its substrate.
file:projects/P_PUTIDA/deep-research/PSEPK__trey_trez_trehalose_biosynthesis__ppu00500-deep-research-openscientist.md
OpenScientist PSEPK ppu00500 satisfiability review
The species-aware review evaluates the TreY/TreZ route in Pseudomonas putida KT2440 against the broader starch-and-sucrose map.
file:PSEPK/treY/treY-ai-review.yaml
PSEPK treY gene review
Q88FN6 is the candidate TreY step.
file:PSEPK/treZ/treZ-ai-review.yaml
PSEPK treZ gene review
Q88FN8 is the candidate TreZ step.
3Nodes
2Parts
0Variant Sets
0Variants
2Annotons
1Connections

Derived QC

Recommended-field compliance

100.0% recommended fields populated

All recommended fields populated.

Module deep research

✗ none found

No MODULE:trey_trez_trehalose_biosynthesis deep-research report alongside the module YAML.

Leaf nodes lacking representative members

✓ every leaf node grounds to a representative protein.

Template conformance

✓ every declared conforms_to bundle matches its template motif.

Gene-review completeness (2/4 grounded genes reviewed)

2 complete review(s) · 0 with deep research · 2 missing review · 2 reviewed but lacking deep research

Gene Review Complete Deep research
Arthrobacter sp. Q36 TreY Q44315 ✗ — —
Arthrobacter sp. Q36 TreZ Q44316 ✗ — —
treY Q88FN6 ✓ ✓ ✗
treZ Q88FN8 ✓ ✓ ✗

Details

TreY/TreZ trehalose biosynthesis from alpha-glucanMetabolic Pathwaytrey_trez_trehalose_biosynthesis
trehalose biosynthetic processGO:0005992

Exact UniProt exemplars delimit both reactions without restricting the module taxonomically. Q44315 and Q44316 are the Arthrobacter sp. Q36 TreY and TreZ proteins associated with the cloned gene pair and the separately purified enzyme activities (PMID:8605217, PMID:8611744, and PMID:8611745). The TreY and TreZ subfamilies are used instead of their much broader alpha-amylase and glucan-enzyme parent families. No molecular function or location is duplicated at module level, and no ancestral PTN node is asserted without verified PAINT IBD evidence.

Connections

treY_step -> treZ_step Provides Input For
TreY supplies maltooligosyltrehalose to TreZ.
Part 1: maltooligosyltrehalose formation
Maltooligosyl trehalose synthaseReactiontreY_step

Annotons

Maltooligosyl trehalose synthase
treY_activity
Participant: Family: TreY maltooligosyl trehalose synthases
Family:
TreY maltooligosyl trehalose synthasesPANTHER:PTHR10357:SF216
Representative Members: PSEPK TreYUniProtKB:Q88FN6 Arthrobacter sp. Q36 TreYUniProtKB:Q44315

Function

(1,4)-alpha-D-glucan 1-alpha-D-glucosylmutase activityGO:0047470
Substrates: alpha-1,4-glucan with a reducing-end alpha-1,4 linkage
Products: maltooligosyltrehalose

Rearranges the reducing-end glycosidic bond to create the trehalose-containing intermediate.

Part 2: trehalose release
Malto-oligosyltrehalose trehalohydrolaseReactiontreZ_step

Annotons

Malto-oligosyltrehalose trehalohydrolase
treZ_activity
Participant: Family: TreZ malto-oligosyltrehalose trehalohydrolases
Family:
TreZ malto-oligosyltrehalose trehalohydrolasesPANTHER:PTHR43651:SF11
Representative Members: PSEPK TreZUniProtKB:Q88FN8 Arthrobacter sp. Q36 TreZUniProtKB:Q44316

Function

4-alpha-D-(1->4)-alpha-D-glucanotrehalose trehalohydrolase activityGO:0033942
Substrates: maltooligosyltrehalose water
Products: trehalose shortened alpha-1,4-glucan

Hydrolyzes the glucosidic linkage adjacent to the trehalose moiety and releases free trehalose.