Type-II fatty-acid synthesis

A reusable dissociated bacterial fatty-acid synthase pathway that forms malonyl-CoA with acetyl-CoA carboxylase, loads malonate onto acyl carrier protein, initiates an acyl-ACP chain, and iteratively elongates that chain through condensation, beta-keto reduction, dehydration, and enoyl reduction. An optional FabA/FabB branch introduces and extends cis unsaturation. Enzyme-family and cofactor variants are represented explicitly rather than collapsed into a type-I fatty-acid synthase.

MODULE:type_ii_fatty_acid_synthesisDRAFTMetabolic Pathwaymodules/type_ii_fatty_acid_synthesis.yaml

fatty acid biosynthetic processGO:0006633

GO:0006633

fatty acid biosynthetic process

GO:0006633 provides the biological-process scope for de novo fatty-acid chain initiation and elongation.

file:modules/type_ii_fatty_acid_synthesis-deep-research-openscientist.md

OpenScientist research for the reusable type-II fatty-acid synthesis module

The module-level review supports the obligatory initiation and four-reaction elongation sequence, while documenting enzyme-family variation and the conditional FabA/FabB unsaturation branch.

file:projects/P_PUTIDA/deep-research/PSEPK__type_ii_fatty_acid_synthesis__ppu00061-deep-research-openscientist.md

OpenScientist PSEPK ppu00061 pathway satisfiability review

The species-aware review finds the KT2440 pathway satisfiable, identifies AcpP and FabV as core members, and flags several same-family paralogs as candidates that must not be assumed to perform the core pathway role.

file:PSEPK/accC/accC-ai-review.yaml

PSEPK accC gene review

The KT2440 review identifies Q88QD6 as the biotin-carboxylase subunit of acetyl-CoA carboxylase.

file:PSEPK/fabD/fabD-uniprot.txt

UniProtKB entry Q88LL7 for PSEPK FabD

UniProt identifies Q88LL7 as malonyl-CoA:ACP transacylase.

file:PSEPK/PP_4379/PP_4379-uniprot.txt

UniProtKB entry Q88ES4 for PSEPK PP_4379

UniProt assigns Q88ES4 beta-ketoacyl-ACP synthase III activity.

file:PSEPK/PP_4545/PP_4545-uniprot.txt

UniProtKB entry Q88EC1 for PSEPK PP_4545

UniProt assigns Q88EC1 beta-ketodecanoyl-ACP synthase activity.

file:PSEPK/PP_0262/PP_0262-uniprot.txt

UniProtKB entry Q88R72 for PSEPK PP_0262

UniProt links Q88R72 to the primary structural and biochemical study of the standalone MadB malonyl-ACP decarboxylase.

PMID:36796578

Initiation of fatty acid biosynthesis in Pseudomonas putida KT2440

Direct genetics and biochemistry establish three initiation routes: short-chain-acyl-CoA-selective FabH1, medium-chain-acyl-CoA-selective FabH2, and MadB-dependent production of acetyl-ACP from malonyl-ACP.

file:PSEPK/fabV/fabV-uniprot.txt

UniProtKB entry Q88E33 for PSEPK FabV

UniProt identifies Q88E33 as the NADH-dependent enoyl-ACP reductase of FAS-II.

27Nodes

14Parts

5Variant Sets

12Variants

18Annotons

11Connections

Derived QC

Recommended-field compliance

100.0% recommended fields populated

All recommended fields populated.

Module deep research

✓ present

type_ii_fatty_acid_synthesis-deep-research-openscientist.md (openscientist)

Leaf nodes lacking representative members

✓ every leaf node grounds to a representative protein.

Template conformance

✓ every declared conforms_to bundle matches its template motif.

Gene-review completeness (15/18 grounded genes reviewed)

15 complete review(s) · 15 with deep research · 3 missing review · 0 reviewed but lacking deep research

Gene	Review	Complete	Deep research
accA Q88MG4	✓	✓	✓
accB Q88QD5	✓	✓	✓
accC Q88QD6	✓	✓	✓
accD Q88LD9	✓	✓	✓
acpP Q88LL5	✓	✓	✓
fabA Q88FC4	✓	✓	✓
fabB Q88FC3	✓	✓	✓
fabD Q88LL7	✓	✓	✓
fabF Q88LL4	✓	✓	✓
fabG Q88LL6	✓	✓	✓
fabV Q88E33	✓	✓	✓
fabZ Q88MG9	✓	✓	✓
Escherichia coli FabI P0AEK4	✗	—	—
Bacillus subtilis FabL P71079	✗	—	—
PP_0262 Q88R72	✓	✓	✓
PP_4379 Q88ES4	✓	✓	✓
PP_4545 Q88EC1	✓	✓	✓
Streptococcus pneumoniae R6 FabK Q8DR17	✗	—	—

Tree

Type-II fatty-acid synthesisMetabolic Pathwaytype_ii_fatty_acid_synthesis

Parts
1. 1. malonyl-CoA formation
2. Acetyl-CoA carboxylaseReactionacetyl_coa_carboxylation
  
  Annotons
  - Heteromeric acetyl-CoA carboxylase Protein Complex: bacterial AccABCD acetyl-CoA carboxylase
3. 2. acyl-group carriage
4. Acyl carrier proteinMolecular Functionacp_carrier_role
  
  Annotons
  - Holo-acyl carrier protein Family: canonical bacterial FAS-II acyl carrier proteins
5. 3. malonyl-ACP formation
6. FabD malonyl-ACP loadingReactionmalonyl_acp_loading
  
  Annotons
  - Malonyl-CoA:ACP transacylase Family: FabD malonyl-CoA:ACP transacylases
7. 4. fatty-acid chain initiation
8. Alternative FAS-II chain-initiation routesMetabolic Pathwaychain_initiation
  
  Variants
  1. Fatty-acid chain-initiation route by primer source and initiating enzyme chemistry (One Or More)
    
    KAS-III acyl-CoA-dependent initiationReactionkasIII_route
    
    Variants
    
    KAS-III acyl-CoA substrate variants by acyl-CoA primer range (One Or More)
    
    FabH1 short-chain-acyl-CoA initiationReactionfabH1_short_chain_variant
    
    Annotons
    
    FabH1 beta-ketoacyl-ACP synthase III Family: FabH-like beta-ketoacyl-ACP synthase III enzymes
    
    FabH2 medium-chain-acyl-CoA initiationReactionfabH2_medium_chain_variant
    
    Annotons
    
    FabH2 beta-ketodecanoyl-ACP synthase Family: medium-chain-acyl-CoA-selective FabH enzymes
    
    Mad/YiiD malonyl-ACP decarboxylase routeMetabolic Pathwaymad_decarboxylase_route
    
    Parts
    
    1. acetyl-ACP primer formation
    
    Malonyl-ACP decarboxylationReactionmad_acetyl_acp_formation
    
    Annotons
    
    Mad/YiiD malonyl-ACP decarboxylase Family: Mad/YiiD-family malonyl-ACP decarboxylases
    
    2. acetyl-ACP condensation
    
    Acetyl-ACP and malonyl-ACP condensationReactionmad_route_first_condensation
    
    Annotons
    
    FabB/FabF-family first condensation Family: FabB/FabF elongation condensing enzymes
9. 5. iterative acyl-chain elongation
10. Four-reaction FAS-II elongation cycleMetabolic Pathwayelongation_cycle
  
  Parts
  1. 1. decarboxylative condensation
  2. KAS-I/KAS-II condensationReactionelongation_condensation
    
    Variants
    
    Elongation condensing-enzyme variants by condensing enzyme (One Or More)
    
    FabB KAS-IReactionfabB_kasI_variant
    
    Annotons
    
    FabB 3-oxoacyl-ACP synthase Family: FabB/KAS-I condensing enzymes
    
    FabF KAS-IIReactionfabF_kasII_variant
    
    Annotons
    
    FabF 3-oxoacyl-ACP synthase Family: FabF/KAS-II condensing enzymes
  3. 2. beta-keto reduction
  4. 3-oxoacyl-ACP reductionReactionbeta_keto_reduction
    
    Annotons
    
    FabG-family 3-oxoacyl-ACP reductase Family: FabG-family 3-oxoacyl-ACP reductases
  5. 3. 3-hydroxyacyl dehydration
  6. 3-hydroxyacyl-ACP dehydrationReactionhydroxyacyl_dehydration
    
    Variants
    
    FAS-II dehydratase variants by dehydratase family (One Or More)
    
    FabZ dehydrataseReactionfabZ_dehydratase_variant
    
    Annotons
    
    FabZ 3-hydroxyacyl-ACP dehydratase Family: FabZ-family FAS-II dehydratases
    
    FabA dehydrataseReactionfabA_dehydratase_variant
    
    Annotons
    
    FabA 3-hydroxyacyl-ACP dehydratase Family: FabA-family dehydratase/isomerases
  7. 4. enoyl reduction
  8. Enoyl-ACP reductionReactionenoyl_reduction
    
    Variants
    
    Enoyl-ACP reductase variants by electron donor and enzyme family (One Or More)
    
    FabI NADH-dependent reductaseReactionfabI_nadh_variant
    
    Annotons
    
    FabI enoyl-ACP reductase Family: FabI-family NADH-dependent enoyl-ACP reductases
    
    FabK flavin-dependent reductaseReactionfabK_nadh_variant
    
    Annotons
    
    FabK enoyl-ACP reductase Family: FabK-family flavin-dependent enoyl-ACP reductases
    
    FabV NADH-dependent reductaseReactionfabV_nadh_variant
    
    Annotons
    
    FabV enoyl-ACP reductase Family: NADH-dependent bacterial enoyl-ACP reductases
    
    FabL-like NADPH-dependent reductaseReactionfabL_nadph_variant
    
    Annotons
    
    FabL-like enoyl-ACP reductase Family: FabL-like NADPH-dependent enoyl-ACP reductases
11. 6. cis-unsaturated fatty-acid branch optional
12. FabA/FabB cis-unsaturated branchMetabolic Pathwaycis_unsaturated_branch
  
  Parts
  1. 1. trans-2 to cis-3 isomerization
  2. FabA trans-2-decenoyl-ACP isomerizationReactionfabA_double_bond_isomerization
    
    Annotons
    
    FabA trans-2-decenoyl-ACP isomerase Family: FabA-family dehydratase/isomerases
  3. 2. cis-unsaturated chain extension
  4. FabB unsaturated-chain condensationReactionfabB_unsaturated_condensation
    
    Annotons
    
    FabB cis-unsaturated chain elongation Family: FabB/KAS-I condensing enzymes

Details

Type-II fatty-acid synthesisMetabolic Pathwaytype_ii_fatty_acid_synthesis

fatty acid biosynthetic processGO:0006633

Exact KT2440 accessions are representative members and do not restrict the module to Pseudomonas. Same-family paralogs without a distinct, supported pathway role remain gene-level curation questions rather than separate module variants. ACP phosphopantetheinylation is required before use but is activation context outside this pathway boundary. FabI is grounded to the functionally appropriate PTHR43159 parent and PAINT node PTN001215190 because the current SF2 name is chloroplast-biased even though the subfamily contains bacterial FabI. PANTHER's assignments for the FabK exemplar (PTHR32332:SF20, a broad 2-nitropropane-dioxygenase-like group) and the FabL exemplar (PTHR43639:SF1, a broad SDR group) are deliberately not used to delimit those variants; experimentally characterized UniProt accessions are retained as exemplars. The existing fatty_acid_de_novo_synthesis module describes type-I mammalian lipogenesis and is intentionally separate.

Connections

acetyl_coa_carboxylation -> malonyl_acp_loading Provides Input For

AccABCD supplies malonyl-CoA to FabD.

acp_carrier_role -> malonyl_acp_loading Provides Input For

Holo-ACP accepts malonate from FabD.

malonyl_acp_loading -> chain_initiation Provides Input For

Malonyl-ACP is the two-carbon donor for chain initiation.

chain_initiation -> elongation_cycle Provides Input For

The initiated beta-ketoacyl-ACP enters reductive elongation.

elongation_cycle -> cis_unsaturated_branch Provides Input For

A C10 trans-2-enoyl-ACP intermediate can enter the FabA/FabB branch.

Part 1: malonyl-CoA formation

Acetyl-CoA carboxylaseReactionacetyl_coa_carboxylation

Annotons

Heteromeric acetyl-CoA carboxylase

accABCD_complex

Participant: Protein Complex: bacterial AccABCD acetyl-CoA carboxylase

Protein Complex:

bacterial AccABCD acetyl-CoA carboxylaseGO:0009317 Heteromeric bacterial complex containing biotin carboxylase AccC, biotin-carrier AccB, and AccA/AccD carboxyltransferase subunits.

Active units:

AccC biotin carboxylase

Participant: Family: bacterial AccC biotin carboxylases

Family:

bacterial AccC biotin carboxylases

Representative Members: PSEPK AccCUniProtKB:Q88QD6

Role: ATP-dependent carboxylation of the AccB-bound biotin cofactor.

Function:

biotin carboxylase activityGO:0004075

AccB biotin-carrier subunit

Participant: Family: bacterial AccB biotin-carrier proteins

Family:

bacterial AccB biotin-carrier proteins

Representative Members: PSEPK AccBUniProtKB:Q88QD5

Role: Covalently carries biotin between the two half-reactions.

AccA carboxyltransferase alpha subunit

Participant: Family: bacterial AccA carboxyltransferase alpha subunits

Family:

bacterial AccA carboxyltransferase alpha subunits

Representative Members: PSEPK AccAUniProtKB:Q88MG4

Role: Alpha component of the acetyl-CoA carboxyltransferase.

AccD carboxyltransferase beta subunit

Participant: Family: bacterial AccD carboxyltransferase beta subunits

Family:

bacterial AccD carboxyltransferase beta subunits

Representative Members: PSEPK AccDUniProtKB:Q88LD9

Role: Beta component of the acetyl-CoA carboxyltransferase.

Function

acetyl-CoA carboxylase activityGO:0003989

Substrates: acetyl-CoA hydrogencarbonate ATP

Products: malonyl-CoA ADP phosphate

Produces the malonyl-CoA two-carbon donor for FAS-II.

Part 2: acyl-group carriage

Acyl carrier proteinMolecular Functionacp_carrier_role

Annotons

Holo-acyl carrier protein

acpP_carrier

Participant: Family: canonical bacterial FAS-II acyl carrier proteins

Family:

canonical bacterial FAS-II acyl carrier proteinsPANTHER:PTHR20863

Representative Members: PSEPK AcpPUniProtKB:Q88LL5

Function

acyl carrier activityGO:0000036

Carries malonyl and growing acyl chains on a phosphopantetheine prosthetic group between dissociated enzymes.

Part 3: malonyl-ACP formation

FabD malonyl-ACP loadingReactionmalonyl_acp_loading

Annotons

Malonyl-CoA:ACP transacylase

fabD_transacylase

Participant: Family: FabD malonyl-CoA:ACP transacylases

Family:

FabD malonyl-CoA:ACP transacylases

Representative Members: PSEPK FabDUniProtKB:Q88LL7

Function

acyl-carrier-protein S-malonyltransferase activityGO:0004314

Substrates: malonyl-CoA holo-ACP

Products: malonyl-ACP CoA

Loads the two-carbon donor onto ACP.

Part 4: fatty-acid chain initiation

Alternative FAS-II chain-initiation routesMetabolic Pathwaychain_initiation

Bacterial FAS-II systems can form the first beta-ketoacyl-ACP through a FabH-like KAS-III reaction or can first generate an acetyl-ACP primer with a Mad/YiiD-family malonyl-ACP decarboxylase.

Variant set: Fatty-acid chain-initiation route by primer source and initiating enzyme chemistry (One Or More)

KAS-III acyl-CoA-dependent initiationReactionkasIII_route

Variant set: KAS-III acyl-CoA substrate variants by acyl-CoA primer range (One Or More)

FabH1 short-chain-acyl-CoA initiationReactionfabH1_short_chain_variant

Annotons

FabH1 beta-ketoacyl-ACP synthase III

fabH1_initiator

Participant: Family: FabH-like beta-ketoacyl-ACP synthase III enzymes

Family:

FabH-like beta-ketoacyl-ACP synthase III enzymesPANTHER:PTHR34069

Representative Members: PSEPK FabH1UniProtKB:Q88ES4

Function

beta-ketoacyl-acyl-carrier-protein synthase III activityGO:0033818

Substrates: acetyl-CoA malonyl-ACP

Products: acetoacetyl-ACP CoA carbon dioxide

Uses a short-chain acyl-CoA primer to form the first beta-ketoacyl-ACP.

FabH2 medium-chain-acyl-CoA initiationReactionfabH2_medium_chain_variant

Annotons

FabH2 beta-ketodecanoyl-ACP synthase

fabH2_initiator

Participant: Family: medium-chain-acyl-CoA-selective FabH enzymes

Family:

medium-chain-acyl-CoA-selective FabH enzymesPANTHER:PTHR34069

Representative Members: PSEPK FabH2UniProtKB:Q88EC1

Function

beta-ketodecanoyl-acyl-carrier-protein synthase activityGO:0061990

Substrates: octanoyl-CoA malonyl-ACP

Products: 3-oxodecanoyl-ACP CoA carbon dioxide

Uses a medium-chain acyl-CoA primer to enter FAS-II at a correspondingly longer chain length.

Mad/YiiD malonyl-ACP decarboxylase routeMetabolic Pathwaymad_decarboxylase_route

Mad-family decarboxylation creates acetyl-ACP, which a FabB/FabF-like condensing enzyme extends with malonyl-ACP.

Connections

mad_acetyl_acp_formation -> mad_route_first_condensation Provides Input For

Mad-generated acetyl-ACP is the primer for condensation.

Part 1: acetyl-ACP primer formation

Malonyl-ACP decarboxylationReactionmad_acetyl_acp_formation

Annotons

Mad/YiiD malonyl-ACP decarboxylase

mad_malonyl_acp_decarboxylase

Participant: Family: Mad/YiiD-family malonyl-ACP decarboxylases

Family:

Mad/YiiD-family malonyl-ACP decarboxylases

Representative Members: PSEPK MadBUniProtKB:Q88R72

Function

malonyl-ACP decarboxylase activityGO:0016831

Substrates: malonyl-ACP proton

Products: acetyl-ACP carbon dioxide

Produces an ACP-bound acetyl primer without an acyl-CoA.

Part 2: acetyl-ACP condensation

Acetyl-ACP and malonyl-ACP condensationReactionmad_route_first_condensation

Annotons

FabB/FabF-family first condensation

mad_route_kas_condensation

Participant: Family: FabB/FabF elongation condensing enzymes

Family:

FabB/FabF elongation condensing enzymesPANTHER:PTHR11712

Representative Members: PSEPK FabBUniProtKB:Q88FC3 PSEPK FabFUniProtKB:Q88LL4

Function

3-oxoacyl-acyl-carrier-protein synthase activityGO:0004315

Substrates: acetyl-ACP malonyl-ACP

Products: acetoacetyl-ACP holo-ACP carbon dioxide

Converts the Mad-generated primer into the first beta-ketoacyl-ACP.

Part 5: iterative acyl-chain elongation

Four-reaction FAS-II elongation cycleMetabolic Pathwayelongation_cycle

Each round adds two carbons through condensation, beta-keto reduction, dehydration, and enoyl reduction.

Connections

elongation_condensation -> beta_keto_reduction Provides Input For

Condensation produces 3-oxoacyl-ACP for reduction.

beta_keto_reduction -> hydroxyacyl_dehydration Provides Input For

Reduction produces 3-hydroxyacyl-ACP for dehydration.

hydroxyacyl_dehydration -> enoyl_reduction Provides Input For

Dehydration produces trans-2-enoyl-ACP for reduction.

enoyl_reduction -> elongation_condensation Provides Input For

The saturated acyl-ACP product re-enters another elongation round.

Part 1: decarboxylative condensation

KAS-I/KAS-II condensationReactionelongation_condensation

FabB- and FabF-like condensing enzymes are represented as reusable family alternatives. Additional same-function paralogs are adjudicated in gene reviews rather than promoted to separate module variants without a distinct pathway role.

Variant set: Elongation condensing-enzyme variants by condensing enzyme (One Or More)

FabB KAS-IReactionfabB_kasI_variant

Annotons

FabB 3-oxoacyl-ACP synthase

fabB_condensing_activity

Participant: Family: FabB/KAS-I condensing enzymes

Family:

FabB/KAS-I condensing enzymesPANTHER:PTHR11712

Representative Members: PSEPK FabBUniProtKB:Q88FC3

Function

3-oxoacyl-acyl-carrier-protein synthase activityGO:0004315

Substrates: acyl-ACP malonyl-ACP

Products: two-carbon-extended 3-oxoacyl-ACP carbon dioxide

FabF KAS-IIReactionfabF_kasII_variant

Annotons

FabF 3-oxoacyl-ACP synthase

fabF_condensing_activity

Participant: Family: FabF/KAS-II condensing enzymes

Family:

FabF/KAS-II condensing enzymesPANTHER:PTHR11712

Representative Members: PSEPK FabFUniProtKB:Q88LL4

Function

3-oxoacyl-acyl-carrier-protein synthase activityGO:0004315

Substrates: acyl-ACP malonyl-ACP

Products: two-carbon-extended 3-oxoacyl-ACP carbon dioxide

Part 2: beta-keto reduction

3-oxoacyl-ACP reductionReactionbeta_keto_reduction

Annotons

FabG-family 3-oxoacyl-ACP reductase

fabG_reductase

Participant: Family: FabG-family 3-oxoacyl-ACP reductases

Family:

FabG-family 3-oxoacyl-ACP reductasesPANTHER:PTHR42879

Representative Members: PSEPK FabGUniProtKB:Q88LL6

Function

3-oxoacyl-acyl-carrier-protein reductase (NADPH) activityGO:0004316

Substrates: 3-oxoacyl-ACP NADPH

Products: 3-hydroxyacyl-ACP NADP+

The canonical FabG reaction; family assignment alone is insufficient for promoting other SDR paralogs to this role.

Part 3: 3-hydroxyacyl dehydration

3-hydroxyacyl-ACP dehydrationReactionhydroxyacyl_dehydration

Variant set: FAS-II dehydratase variants by dehydratase family (One Or More)

FabZ dehydrataseReactionfabZ_dehydratase_variant

Annotons

FabZ 3-hydroxyacyl-ACP dehydratase

fabZ_dehydratase

Participant: Family: FabZ-family FAS-II dehydratases

Family:

FabZ-family FAS-II dehydratasesPANTHER:PTHR30272

Representative Members: PSEPK FabZUniProtKB:Q88MG9

Function

(3R)-hydroxyacyl-acyl-carrier-protein dehydratase activityGO:0019171

Substrates: 3-hydroxyacyl-ACP

Products: trans-2-enoyl-ACP H2O

FabA dehydrataseReactionfabA_dehydratase_variant

Annotons

FabA 3-hydroxyacyl-ACP dehydratase

fabA_dehydratase

Participant: Family: FabA-family dehydratase/isomerases

Family:

FabA-family dehydratase/isomerasesPANTHER:PTHR30272

Representative Members: PSEPK FabAUniProtKB:Q88FC4

Function

(3R)-hydroxyacyl-acyl-carrier-protein dehydratase activityGO:0019171

Substrates: 3-hydroxyacyl-ACP

Products: trans-2-enoyl-ACP H2O

Part 4: enoyl reduction

Enoyl-ACP reductionReactionenoyl_reduction

Variant set: Enoyl-ACP reductase variants by electron donor and enzyme family (One Or More)

FabI NADH-dependent reductaseReactionfabI_nadh_variant

Annotons

FabI enoyl-ACP reductase

fabI_reductase

Participant: Family: FabI-family NADH-dependent enoyl-ACP reductases

Family:

FabI-family NADH-dependent enoyl-ACP reductasesPANTHER:PTHR43159

Representative Members: Escherichia coli FabIUniProtKB:P0AEK4

Function

enoyl-acyl-carrier-protein reductase (NADH) activityGO:0004318

Substrates: trans-2-enoyl-ACP NADH

Products: saturated acyl-ACP NAD+

FabK flavin-dependent reductaseReactionfabK_nadh_variant

Annotons

FabK enoyl-ACP reductase

fabK_reductase

Participant: Family: FabK-family flavin-dependent enoyl-ACP reductases

Family:

FabK-family flavin-dependent enoyl-ACP reductases

Representative Members: Streptococcus pneumoniae R6 FabKUniProtKB:Q8DR17

Function

enoyl-acyl-carrier-protein reductase (NADH) activityGO:0004318

Substrates: trans-2-enoyl-ACP NADH

Products: saturated acyl-ACP NAD+

Cofactors: FMN

FabV NADH-dependent reductaseReactionfabV_nadh_variant

Annotons

FabV enoyl-ACP reductase

fabV_reductase

Participant: Family: NADH-dependent bacterial enoyl-ACP reductases

Family:

NADH-dependent bacterial enoyl-ACP reductasesPANTHER:PTHR37480:SF1

Representative Members: PSEPK FabVUniProtKB:Q88E33

Function

enoyl-acyl-carrier-protein reductase (NADH) activityGO:0004318

Substrates: trans-2-enoyl-ACP NADH

Products: saturated acyl-ACP NAD+

FabL-like NADPH-dependent reductaseReactionfabL_nadph_variant

Annotons

FabL-like enoyl-ACP reductase

fabL_reductase

Participant: Family: FabL-like NADPH-dependent enoyl-ACP reductases

Family:

FabL-like NADPH-dependent enoyl-ACP reductases

Representative Members: Bacillus subtilis FabLUniProtKB:P71079

Function

enoyl-acyl-carrier-protein reductase (NADPH) activityGO:0141148

Substrates: trans-2-enoyl-ACP NADPH

Products: saturated acyl-ACP NADP+

Part 6: cis-unsaturated fatty-acid branch (optional)

FabA/FabB cis-unsaturated branchMetabolic Pathwaycis_unsaturated_branch

Connections

fabA_double_bond_isomerization -> fabB_unsaturated_condensation Provides Input For

FabA supplies cis-3-decenoyl-ACP to FabB.

Part 1: trans-2 to cis-3 isomerization

FabA trans-2-decenoyl-ACP isomerizationReactionfabA_double_bond_isomerization

Annotons

FabA trans-2-decenoyl-ACP isomerase

fabA_isomerase

Participant: Family: FabA-family dehydratase/isomerases

Family:

FabA-family dehydratase/isomerasesPANTHER:PTHR30272

Representative Members: PSEPK FabAUniProtKB:Q88FC4

Function

trans-2-decenoyl-acyl-carrier-protein isomerase activityGO:0034017

Creates cis-3-decenoyl-ACP while bypassing enoyl reduction.

Part 2: cis-unsaturated chain extension

FabB unsaturated-chain condensationReactionfabB_unsaturated_condensation

Annotons

FabB cis-unsaturated chain elongation

fabB_unsaturated_branch

Participant: Family: FabB/KAS-I condensing enzymes

Family:

FabB/KAS-I condensing enzymesPANTHER:PTHR11712

Representative Members: PSEPK FabBUniProtKB:Q88FC3

Function

3-oxoacyl-acyl-carrier-protein synthase activityGO:0004315

Extends cis-3-decenoyl-ACP while preserving cis unsaturation.