The minimal, reusable vesicle-budding motif: a membrane-associated small GTPase (Arf or Sar1, switched on by its GEF) nucleates assembly of a protein coat on the donor membrane; coat adaptors capture transmembrane cargo and concentrate it into the forming bud; polymerization of the coat deforms the membrane and drives scission of a coated vesicle; and the coat is then shed (uncoating, often on GTP hydrolysis) to yield a transport-competent vesicle. The same motif is reused by the COPII (ER exit), COPI (Golgi-to-ER), and clathrin (post-Golgi/ endocytic) systems. The motif is gene-free and taxon-neutral, fixing only the coat, adaptor, and budding roles and the recruit -> capture -> bud -> uncoat topology. Grounded in GO:0006900 (vesicle budding from membrane).
Reusable conformance target. A node declares `conforms_to: [{template: vesicle_coat_budding}]` when a coat is recruited (typically by an Arf/Sar1 GTPase), captures cargo, deforms the membrane to bud a vesicle, and is shed. The coat identity (COPII/COPI/clathrin) and cargo are realization-specific. The GTPase-priming step is itself an instance of MODULE:gtpase_switch.
All recommended fields populated.
✗ none found
No MODULE:vesicle_coat_budding deep-research report alongside the module YAML.
4 leaf node(s) with no concrete protein grounding:
✓ every declared conforms_to bundle matches its template motif.
✓ every PRECEDES step chains, or its break is acknowledged via chaining_status.
0 gene-function check(s) reflected in core functions · 0 conflict(s) · 0 evidence or coverage gap(s) (2 comparison(s))
Retained noncore functions are reported separately; they do not require promotion to core functions. Support from representative genes does not establish conservation across the whole family. Missing reviews or identifiers leave a claim unverified.
| Annoton | Participant | Function | Assessment |
|---|---|---|---|
| coat_activity | ANY_WITH_FUNCTION Gene |
structural molecule activity GO:0005198 |
Participant Unchecked
No direct UniProt participant to compare. Abstract selectors and complex-wide activities cannot be inferred from donor or subunit gene reviews. |
| cargo_adaptor_activity | ANY_WITH_FUNCTION Gene |
protein-macromolecule adaptor activity GO:0030674 |
Participant Unchecked
No direct UniProt participant to compare. Abstract selectors and complex-wide activities cannot be inferred from donor or subunit gene reviews. |
No concrete UniProt-grounded genes in this module.
An activated Arf/Sar1 GTPase recruits coat subunits to the donor membrane.
Nucleates the protein coat on the donor membrane.
Coat adaptors select transmembrane cargo and concentrate it into the forming bud.
Selects and concentrates cargo into the bud.
Coat polymerization deforms the membrane and drives scission of a coated vesicle.
Deforms the membrane and releases the coated vesicle.
The coat is shed (often on GTP hydrolysis) to yield a transport-competent vesicle.
Sheds the coat to expose the fusion machinery.