Wzy-dependent O-antigen chain-length control

A structural regulation module for Wzy-dependent O-antigen biosynthesis in which the inner-membrane Wzz-family polysaccharide co-polymerase FepE controls very-long modal O-antigen chain length. FepE is a PCP-1 family scaffold/regulator of Wzy polymerase processivity and is not a protein kinase or ferric enterobactin transporter.

MODULE:wzy_dependent_o_antigen_chain_length_controlDRAFTCONCRETEBiological Processmodules/wzy_dependent_o_antigen_chain_length_control.yaml
O antigen biosynthetic processGO:0009243
GO:0009243
O antigen biosynthetic process
Defines the Wzy-dependent O-antigen process regulated by FepE.
file:genes/ECOLI/fepE/fepE-ai-review.yaml
ECOLI fepE gene review
The completed E. coli fepE review grounds FepE as a Wzz/PCP-1 O-antigen modal chain-length regulator.
PMID:12603743
Regulation of Salmonella typhimurium lipopolysaccharide O antigen chain length is required for virulence; identification of FepE as a second Wzz.
Identifies FepE as the Wzz protein responsible for very-long modal O-antigen chains.
PMID:22437828
Structural characterization of closely related O-antigen lipopolysaccharide (LPS) chain length regulators.
Supports the FepE PCP-1 structural model for modal chain-length regulation.

The module name uses the pathway class rather than E. coli K-12 O-antigen output, because standard K-12 lacks an intact O-antigen locus. The reviewed FepE function is a conserved Wzz-family chain-length regulatory activity.

1Nodes
0Parts
0Variant Sets
0Variants
1Annotons
0Connections

Derived QC

Recommended-field compliance

100.0% recommended fields populated

All recommended fields populated.

Module deep research

✗ none found

No MODULE:wzy_dependent_o_antigen_chain_length_control deep-research report alongside the module YAML.

Leaf nodes lacking representative members

✓ every leaf node grounds to a representative protein.

Template conformance

✓ every declared conforms_to bundle matches its template motif.

Function consistency and core coverage

0 gene-function check(s) reflected in core functions · 0 conflict(s) · 1 evidence or coverage gap(s) (1 comparison(s))

Retained noncore functions are reported separately; they do not require promotion to core functions. Support from representative genes does not establish conservation across the whole family. Missing reviews or identifiers leave a claim unverified.

AnnotonParticipantFunctionAssessment
fepe_chain_length_regulator E. coli FepE
UniProtKB:P26266
Gene 		O-antigen chain-length structural regulator activity
No function ID 		No Function Id
A gene review exists, but this function lacks a GO identifier. Free-text chemistry is not automatically compared.

Gene-review completeness (1/1 grounded genes reviewed)

1 complete review(s) · 1 with deep research · 0 missing review · 0 reviewed but lacking deep research

Gene Review Complete Deep research
fepE P26266 ✓ ✓ ✓

Details

Context
bacteriaNCBITaxon:2
Wzy-dependent O-antigen chain-length controlBiological Processwzy_dependent_o_antigen_chain_length_control
O antigen biosynthetic processGO:0009243
Context
bacteriaNCBITaxon:2

Annotons

FepE very-long O-antigen chain-length regulator
fepe_chain_length_regulator
Participant: Gene Product: E. coli FepE
Gene Product:
E. coli FepEUniProtKB:P26266

Function

O-antigen chain-length structural regulator activity FepE's specific Wzz/PCP structural regulator activity has no exact GO term yet; GO:0005198 structural molecule activity is only the proposed parent for the new term.

Processes

O antigen biosynthetic processGO:0009243

Locations

plasma membraneGO:0005886

Inner-membrane Wzz/PCP-1 oligomer that tunes Wzy-dependent O-antigen polymerization toward the very-long modal chain-length class.