YrhB ATP-independent chaperone-like refolding

A draft single-protein module for the small E. coli YrhB protein, which has been reported to prevent heat-induced PurK aggregation and support refolding of a model client without ATP. The purified K-12 and BL21(DE3) proteins are identical, but the physiological client set and whether this is a native chaperone function rather than an overexpression-associated moonlighting behavior remain unresolved.

MODULE:yrhb_chaperone_like_refoldingDRAFTCONCRETEBiological Processmodules/yrhb_chaperone_like_refolding.yaml
protein folding chaperoneGO:0044183
GO:0044183
protein folding chaperone
The closest current GO molecular-function term for YrhB's chaperone-like activity.
GO:0042026
protein refolding
Defines the refolding process supported by YrhB in vitro.
file:genes/ECOLI/yrhB/yrhB-ai-review.yaml
ECOLI yrhB gene review
The completed E. coli yrhB review grounds YrhB as an ATP-independent chaperone-like protein and rejects unsupported Imm35 immunity assertions.
PMID:22569261
YrhB is a highly stable small protein with unique chaperone-like activity in Escherichia coli BL21(DE3).
Reports heat-induced aggregation prevention, refolding assistance, and ATP independence for YrhB.

No adjacent cognate toxin gene exists in E. coli K-12, so this module does not include the predicted Imm35 peptidase-inhibitor/polymorphic-toxin immunity activity.

1Nodes
0Parts
0Variant Sets
0Variants
1Annotons
0Connections

Derived QC

Recommended-field compliance

100.0% recommended fields populated

All recommended fields populated.

Module deep research

✗ none found

No MODULE:yrhb_chaperone_like_refolding deep-research report alongside the module YAML.

Leaf nodes lacking representative members

✓ every leaf node grounds to a representative protein.

Template conformance

✓ every declared conforms_to bundle matches its template motif.

Function consistency and core coverage

1 gene-function check(s) reflected in core functions · 0 conflict(s) · 0 evidence or coverage gap(s) (1 comparison(s))

Retained noncore functions are reported separately; they do not require promotion to core functions. Support from representative genes does not establish conservation across the whole family. Missing reviews or identifiers leave a claim unverified.

AnnotonParticipantFunctionAssessment
yrhb_chaperone_like_activity E. coli YrhB
UniProtKB:P46857
Gene 		protein folding chaperone
GO:0044183 		Core Supported
The GO molecular function is reflected in core_functions (exact term or a reviewed subclass).

Gene-review completeness (1/1 grounded genes reviewed)

1 complete review(s) · 1 with deep research · 0 missing review · 0 reviewed but lacking deep research

Gene Review Complete Deep research
yrhB P46857 ✓ ✓ ✓

Details

Context
Escherichia coliNCBITaxon:83333
YrhB ATP-independent chaperone-like refoldingBiological Processyrhb_chaperone_like_refolding
protein folding chaperoneGO:0044183
Context
Escherichia coliNCBITaxon:83333

Annotons

YrhB chaperone-like activity
yrhb_chaperone_like_activity
Participant: Gene Product: E. coli YrhB
Gene Product:
E. coli YrhBUniProtKB:P46857

Function

protein folding chaperoneGO:0044183
Substrates: heat-denatured protein client
Products: refolding-competent protein client

Processes

protein refoldingGO:0042026

Locations

cytoplasmGO:0005737

Small ATP-independent chaperone-like protein shown in vitro to prevent PurK aggregation and promote refolding of a model client.