MetaboLights MTBLS1 → GO function enrichment (via Rhea + rhea2go)

MetaboLights MTBLS1 → GO function enrichment (via Rhea + rhea2go)

Input: MetaboLights MTBLS1, 64 metabolites (Salek et al.; urine NMR, T2D vs control)

Generated by go_enrichment.py — closure-aware
hypergeometric (Fisher) over-representation of GO terms reached from the
metabolite set through Rhea + rhea2go, with is_a/part_of closure from
go-basic.obo. All inputs fetched live; nothing hardcoded.

Top enriched GO terms

GO term Aspect k/n K/N Fold FDR
GO:0016810 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds MF 38/64 513/7174 8.3× 8.6e-25
GO:0016811 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides MF 32/64 354/7174 10.1× 8.7e-23
GO:0008131 primary methylamine oxidase activity MF 22/64 119/7174 20.7× 1.4e-21
GO:0016641 oxidoreductase activity, acting on the CH-NH2 group of donors, oxygen as acceptor MF 23/64 141/7174 18.3× 1.7e-21
GO:0016874 ligase activity MF 32/64 456/7174 7.9× 1.0e-19
GO:0016638 oxidoreductase activity, acting on the CH-NH2 group of donors MF 23/64 183/7174 14.1× 5.7e-19
GO:0001716 L-amino-acid oxidase activity MF 14/64 52/7174 30.2× 9.0e-16
GO:0016787 hydrolase activity MF 48/64 1839/7174 2.9× 1.8e-14
GO:0047661 amino-acid racemase activity MF 13/64 57/7174 25.6× 1.3e-13
GO:0016855 racemase and epimerase activity, acting on amino acids and derivatives MF 13/64 69/7174 21.1× 1.7e-12
GO:0140385 amino acid transaminase activity MF 13/64 73/7174 20.0× 3.4e-12
GO:0015291 secondary active transmembrane transporter activity MF 12/64 57/7174 23.6× 3.8e-12
GO:0005310 dicarboxylic acid transmembrane transporter activity MF 8/64 14/7174 64.1× 5.8e-12
GO:0003884 D-amino-acid oxidase activity MF 11/64 48/7174 25.7× 1.5e-11
GO:0015297 antiporter activity MF 10/64 36/7174 31.1× 2.2e-11
GO:0050018 amino-acid dehydrogenase [NAD(P)+] activity MF 10/64 41/7174 27.3× 8.9e-11
GO:0047635 L-alanine:oxo-acid transaminase activity MF 10/64 42/7174 26.7× 1.1e-10
GO:0015556 C4-dicarboxylate transmembrane transporter activity MF 7/64 12/7174 65.4× 1.4e-10
GO:0046943 carboxylic acid transmembrane transporter activity MF 10/64 44/7174 25.5× 1.6e-10
GO:0016854 racemase and epimerase activity MF 15/64 165/7174 10.2× 4.4e-10
GO:0004084 branched-chain-amino-acid:2-oxoglutarate transaminase activity MF 6/64 8/7174 84.1× 5.4e-10
GO:0016639 oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor MF 10/64 51/7174 22.0× 6.9e-10
GO:0160215 deacylase activity MF 17/64 260/7174 7.3× 2.6e-09
GO:0016790 thiolester hydrolase activity MF 15/64 196/7174 8.6× 4.4e-09
GO:0016769 transferase activity, transferring nitrogenous groups MF 14/64 164/7174 9.6× 4.5e-09
GO:0016903 oxidoreductase activity, acting on the aldehyde or oxo group of donors MF 19/64 356/7174 6.0× 4.5e-09
GO:0016620 oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor MF 18/64 313/7174 6.4× 4.5e-09
GO:0016774 phosphotransferase activity, carboxyl group as acceptor MF 8/64 31/7174 28.9× 6.3e-09
GO:0016830 carbon-carbon lyase activity MF 20/64 415/7174 5.4× 7.3e-09
GO:0016823 hydrolase activity, acting on carbon-carbon bonds, in ketonic substances MF 9/64 48/7174 21.0× 8.6e-09

Reading this

k/n = foreground metabolites annotated to the term / foreground size;
K/N = same in the background universe; Fold = (k/n)/(K/N). Terms reached
via rhea2go are GO molecular function; closure lifts them to more general
function terms. BP-level lift via the enzyme/gene layer (GOA / GO-CAM) is the
documented next step (see the project page).

Method / reproducibility