# Manual review of HTP-family vesicle-type rows, 2026-10-10. Source: decisions_draft.py - gene: human/AARS1 row_index: 44 term: GO:0070062 extracellular exosome reference: PMID:20458337 previous_action: UNDECIDED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic aminoacyl-tRNA synthetase. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/ABCB4 row_index: 48 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: UNDECIDED decision: KEEP_AS_NON_CORE changed: true basis: Canalicular plasma-membrane phospholipid flippase. Transmembrane/lipid-anchored protein of the plasma membrane or endosomal system (UniProt); such proteins are expected EV contents (MISEV2018 (PMID:30637094) category 1). True, but not its core location. - gene: human/ACLY row_index: 33 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic ATP-citrate lyase. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/ACLY row_index: 35 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic ATP-citrate lyase. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/ACLY row_index: 39 term: GO:0070062 extracellular exosome reference: PMID:20458337 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic ATP-citrate lyase. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/ACSL4 row_index: 43 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: UNDECIDED decision: MARK_AS_OVER_ANNOTATED changed: true basis: UniProt places ACSL4 in mitochondrial outer, peroxisomal and ER membranes. Mainly resident in an intracellular compartment that MISEV2018 (PMID:30637094) lists as not enriched in small EVs of plasma-membrane/endosomal origin (category 4), and its own review keeps no experimental cell-surface or plasma-membrane pool; an exosome annotation from a bulk EV proteome is not supported. - gene: human/ACSL4 row_index: 44 term: GO:0070062 extracellular exosome reference: PMID:20458337 previous_action: UNDECIDED decision: MARK_AS_OVER_ANNOTATED changed: true basis: UniProt places ACSL4 in mitochondrial outer, peroxisomal and ER membranes. Mainly resident in an intracellular compartment that MISEV2018 (PMID:30637094) lists as not enriched in small EVs of plasma-membrane/endosomal origin (category 4), and its own review keeps no experimental cell-surface or plasma-membrane pool; an exosome annotation from a bulk EV proteome is not supported. - gene: human/ACTA1 row_index: 25 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: UNDECIDED decision: KEEP_AS_NON_CORE changed: true basis: Actin. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/ACTA1 row_index: 26 term: GO:0072562 blood microparticle reference: PMID:22516433 previous_action: UNDECIDED decision: KEEP_AS_NON_CORE changed: true basis: Actin. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/ACTA1 row_index: 28 term: GO:0070062 extracellular exosome reference: PMID:19199708 previous_action: UNDECIDED decision: KEEP_AS_NON_CORE changed: true basis: Actin. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/ACTA1 row_index: 29 term: GO:0070062 extracellular exosome reference: PMID:21362503 previous_action: UNDECIDED decision: KEEP_AS_NON_CORE changed: true basis: Actin. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/ACTB row_index: 215 term: GO:0070062 extracellular exosome reference: PMID:11487543 previous_action: UNDECIDED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic actin. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/ACTB row_index: 222 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: UNDECIDED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic actin. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/ACTB row_index: 230 term: GO:0072562 blood microparticle reference: PMID:22516433 previous_action: UNDECIDED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic actin. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/ACTB row_index: 233 term: GO:0070062 extracellular exosome reference: PMID:19199708 previous_action: UNDECIDED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic actin. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/ACTB row_index: 234 term: GO:0070062 extracellular exosome reference: PMID:20458337 previous_action: UNDECIDED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic actin. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/ACTG2 row_index: 16 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Smooth-muscle actin. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/ACTG2 row_index: 17 term: GO:0072562 blood microparticle reference: PMID:22516433 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Smooth-muscle actin. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/ACTR1A row_index: 36 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Dynactin Arp1 subunit. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/ACTR1A row_index: 37 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Dynactin Arp1 subunit. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/ACTR1B row_index: 15 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Dynactin Arp1 subunit. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/ADGRV1 row_index: 48 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: UNDECIDED decision: KEEP_AS_NON_CORE changed: true basis: Plasma-membrane adhesion GPCR. Transmembrane/lipid-anchored protein of the plasma membrane or endosomal system (UniProt); such proteins are expected EV contents (MISEV2018 (PMID:30637094) category 1). True, but not its core location. - gene: human/AGT row_index: 74 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: MARK_AS_OVER_ANNOTATED changed: false basis: Secreted angiotensinogen. Secreted or lumenal protein (UniProt). MISEV2018 (PMID:30637094) category 5 allows such proteins onto EVs through specific or promiscuous (proteoglycan, lipid) receptors on the EV surface, but asks that their identification be backed by evidence of that association. A bulk EV proteome cannot separate surface binding from co-isolation, and this protein's annotations record no experimental proteoglycan, heparin or lipid binding, and its own review keeps no experimental cell-surface or plasma-membrane pool. - gene: human/AGT row_index: 76 term: GO:0072562 blood microparticle reference: PMID:22516433 previous_action: MARK_AS_OVER_ANNOTATED decision: MARK_AS_OVER_ANNOTATED changed: false basis: Secreted angiotensinogen. Secreted or lumenal protein (UniProt). MISEV2018 (PMID:30637094) category 5 allows such proteins onto EVs through specific or promiscuous (proteoglycan, lipid) receptors on the EV surface, but asks that their identification be backed by evidence of that association. A bulk EV proteome cannot separate surface binding from co-isolation, and this protein's annotations record no experimental proteoglycan, heparin or lipid binding, and its own review keeps no experimental cell-surface or plasma-membrane pool. - gene: human/AHCTF1 row_index: 35 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: MARK_AS_OVER_ANNOTATED changed: false basis: Nuclear pore assembly factor (nucleus, nuclear envelope). Mainly resident in an intracellular compartment that MISEV2018 (PMID:30637094) lists as not enriched in small EVs of plasma-membrane/endosomal origin (category 4), and its own review keeps no experimental cell-surface or plasma-membrane pool; an exosome annotation from a bulk EV proteome is not supported. - gene: human/AHCY row_index: 19 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: UNDECIDED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic adenosylhomocysteinase. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/AHCY row_index: 22 term: GO:0070062 extracellular exosome reference: PMID:20458337 previous_action: UNDECIDED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic adenosylhomocysteinase. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/AK2 row_index: 28 term: GO:0070062 extracellular exosome reference: PMID:20458337 previous_action: UNDECIDED decision: MARK_AS_OVER_ANNOTATED changed: true basis: Mitochondrial intermembrane-space adenylate kinase. Mainly resident in an intracellular compartment that MISEV2018 (PMID:30637094) lists as not enriched in small EVs of plasma-membrane/endosomal origin (category 4), and its own review keeps no experimental cell-surface or plasma-membrane pool; an exosome annotation from a bulk EV proteome is not supported. - gene: human/ALB row_index: 52 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: UNDECIDED decision: MARK_AS_OVER_ANNOTATED changed: true basis: Serum albumin, which MISEV2018 (PMID:30637094) names as a negative marker for EVs from plasma, serum and serum-containing culture. Here, urinary exosomes, where filtered plasma albumin is abundant. MISEV2018 category 3a covers non-EV structures that co-isolate with EVs, chiefly lipoproteins and albumin, from plasma, serum and serum-containing culture; detection does not establish EV localization. - gene: human/ALB row_index: 53 term: GO:0070062 extracellular exosome reference: PMID:20458337 previous_action: UNDECIDED decision: MARK_AS_OVER_ANNOTATED changed: true basis: Serum albumin, which MISEV2018 (PMID:30637094) names as a negative marker for EVs from plasma, serum and serum-containing culture. Here, exosomes from cultured B cells, where serum albumin in the medium is a recognised co-isolate. MISEV2018 category 3a covers non-EV structures that co-isolate with EVs, chiefly lipoproteins and albumin, from plasma, serum and serum-containing culture; detection does not establish EV localization. - gene: human/ALB row_index: 54 term: GO:0070062 extracellular exosome reference: PMID:21362503 previous_action: UNDECIDED decision: MARK_AS_OVER_ANNOTATED changed: true basis: Serum albumin, which MISEV2018 (PMID:30637094) names as a negative marker for EVs from plasma, serum and serum-containing culture. Here, exosomes from cultured trabecular meshwork cells, where serum albumin in the medium is a recognised co-isolate. MISEV2018 category 3a covers non-EV structures that co-isolate with EVs, chiefly lipoproteins and albumin, from plasma, serum and serum-containing culture; detection does not establish EV localization. - gene: human/ALB row_index: 55 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: UNDECIDED decision: MARK_AS_OVER_ANNOTATED changed: true basis: Serum albumin, which MISEV2018 (PMID:30637094) names as a negative marker for EVs from plasma, serum and serum-containing culture. Here, exosomes from expressed prostatic secretions in urine. MISEV2018 category 3a covers non-EV structures that co-isolate with EVs, chiefly lipoproteins and albumin, from plasma, serum and serum-containing culture; detection does not establish EV localization. - gene: human/ALB row_index: 57 term: GO:0072562 blood microparticle reference: PMID:22516433 previous_action: UNDECIDED decision: MARK_AS_OVER_ANNOTATED changed: true basis: Serum albumin, which MISEV2018 (PMID:30637094) names as a negative marker for EVs from plasma, serum and serum-containing culture. Here, microvesicles isolated from plasma, the sample MISEV's marker is meant for. MISEV2018 category 3a covers non-EV structures that co-isolate with EVs, chiefly lipoproteins and albumin, from plasma, serum and serum-containing culture; detection does not establish EV localization. - gene: human/ALDH7A1 row_index: 36 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: UNDECIDED decision: KEEP_AS_NON_CORE changed: true basis: 'Cytosolic isoform (UniProt isoform 2: cytosol). Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location.' - gene: human/ALDOB row_index: 36 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: UNDECIDED decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic aldolase. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/ALK row_index: 105 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: UNDECIDED decision: KEEP_AS_NON_CORE changed: true basis: Plasma-membrane receptor tyrosine kinase. Transmembrane/lipid-anchored protein of the plasma membrane or endosomal system (UniProt); such proteins are expected EV contents (MISEV2018 (PMID:30637094) category 1). True, but not its core location. - gene: human/ALPL row_index: 66 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: UNDECIDED decision: KEEP_AS_NON_CORE changed: true basis: GPI-anchored plasma-membrane alkaline phosphatase. Transmembrane/lipid-anchored protein of the plasma membrane or endosomal system (UniProt); such proteins are expected EV contents (MISEV2018 (PMID:30637094) category 1). True, but not its core location. - gene: human/ANXA11 row_index: 66 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: UNDECIDED decision: KEEP_AS_NON_CORE changed: true basis: Calcium-dependent membrane-binding annexin; annexins are classic category 2a EV proteins. Cytosolic protein with lipid or membrane-protein binding ability; MISEV2018 (PMID:30637094) category 2a lists such proteins (ESCRT components, ALIX, annexins, HSPA8 and others) as expected EV contents. True, but not its core location. - gene: human/ANXA11 row_index: 67 term: GO:0070062 extracellular exosome reference: PMID:20458337 previous_action: UNDECIDED decision: KEEP_AS_NON_CORE changed: true basis: Calcium-dependent membrane-binding annexin; annexins are classic category 2a EV proteins. Cytosolic protein with lipid or membrane-protein binding ability; MISEV2018 (PMID:30637094) category 2a lists such proteins (ESCRT components, ALIX, annexins, HSPA8 and others) as expected EV contents. True, but not its core location. - gene: human/ANXA11 row_index: 68 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: UNDECIDED decision: KEEP_AS_NON_CORE changed: true basis: Calcium-dependent membrane-binding annexin; annexins are classic category 2a EV proteins. Cytosolic protein with lipid or membrane-protein binding ability; MISEV2018 (PMID:30637094) category 2a lists such proteins (ESCRT components, ALIX, annexins, HSPA8 and others) as expected EV contents. True, but not its core location. - gene: human/AP2M1 row_index: 168 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: UNDECIDED decision: KEEP_AS_NON_CORE changed: true basis: Plasma-membrane clathrin adaptor subunit (peripheral, cytosolic face). Cytosolic protein with lipid or membrane-protein binding ability; MISEV2018 (PMID:30637094) category 2a lists such proteins (ESCRT components, ALIX, annexins, HSPA8 and others) as expected EV contents. True, but not its core location. - gene: human/AP4M1 row_index: 47 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: MARK_AS_OVER_ANNOTATED changed: false basis: AP-4 coat subunit of the trans-Golgi network (UniProt ECO:0000269). Mainly resident in an intracellular compartment that MISEV2018 (PMID:30637094) lists as not enriched in small EVs of plasma-membrane/endosomal origin (category 4), and its own review keeps no experimental cell-surface or plasma-membrane pool; an exosome annotation from a bulk EV proteome is not supported. - gene: human/APAF1 row_index: 60 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic apoptosome scaffold. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/APOB row_index: 146 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: UNDECIDED decision: MARK_AS_OVER_ANNOTATED changed: true basis: 'Apolipoprotein B, which MISEV2018 (PMID:30637094) names as a negative marker. Here, exosomes from expressed prostatic secretions in urine: APOB-containing lipoproteins are large and are not normally filtered into urine, so this detection is more likely co-isolated plasma or seminal-fluid material than EV cargo. MISEV2018 category 3a covers non-EV structures that co-isolate with EVs, chiefly lipoproteins and albumin, from plasma, serum and serum-containing culture; detection does not establish EV localization.' - gene: human/APOE row_index: 209 term: GO:0072562 blood microparticle reference: PMID:22516433 previous_action: ACCEPT decision: MARK_AS_OVER_ANNOTATED changed: true basis: Apolipoprotein E recovered from a plasma microvesicle preparation. MISEV2018 (PMID:30637094) names APOA1/2 and APOB, not APOE, as negative markers, but APOE is carried on the same plasma lipoproteins, so this sample cannot separate the two. The earlier ACCEPT described APOE's lipoprotein biology, not EV localization. (APOE's other vesicle rows, from non-plasma samples, are KEEP_AS_NON_CORE; see decisions_settled.py.) MISEV2018 category 3a covers non-EV structures that co-isolate with EVs, chiefly lipoproteins and albumin, from plasma, serum and serum-containing culture; detection does not establish EV localization. - gene: human/APOL1 row_index: 18 term: GO:0072562 blood microparticle reference: PMID:22516433 previous_action: UNDECIDED decision: MARK_AS_OVER_ANNOTATED changed: true basis: HDL-associated apolipoprotein L1, recovered from a plasma microvesicle preparation. MISEV2018 (PMID:30637094) category 3a covers non-EV structures that co-isolate with EVs, chiefly lipoproteins and albumin, from plasma, serum and serum-containing culture; detection does not establish EV localization. - gene: human/APP row_index: 353 term: GO:0070062 extracellular exosome reference: PMID:19199708 previous_action: ACCEPT decision: KEEP_AS_NON_CORE changed: true basis: Plasma-membrane/endosomal type I membrane protein; the earlier ACCEPT gave no basis for exosomes being a core location. Transmembrane/lipid-anchored protein of the plasma membrane or endosomal system (UniProt); such proteins are expected EV contents (MISEV2018 (PMID:30637094) category 1). True, but not its core location. - gene: human/APPL2 row_index: 108 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Peripheral early-endosome adaptor. Cytosolic protein with lipid or membrane-protein binding ability; MISEV2018 (PMID:30637094) category 2a lists such proteins (ESCRT components, ALIX, annexins, HSPA8 and others) as expected EV contents. True, but not its core location. - gene: human/APRT row_index: 22 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic salvage enzyme. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/APRT row_index: 23 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic salvage enzyme. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/APRT row_index: 24 term: GO:0070062 extracellular exosome reference: PMID:20458337 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic salvage enzyme. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/ARF1 row_index: 44 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Myristoylated cytosolic small GTPase that cycles onto membranes. Cytosolic protein with lipid or membrane-protein binding ability; MISEV2018 (PMID:30637094) category 2a lists such proteins (ESCRT components, ALIX, annexins, HSPA8 and others) as expected EV contents. True, but not its core location. - gene: human/ARF1 row_index: 50 term: GO:0070062 extracellular exosome reference: PMID:19199708 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Myristoylated cytosolic small GTPase that cycles onto membranes. Cytosolic protein with lipid or membrane-protein binding ability; MISEV2018 (PMID:30637094) category 2a lists such proteins (ESCRT components, ALIX, annexins, HSPA8 and others) as expected EV contents. True, but not its core location. - gene: human/ARF1 row_index: 51 term: GO:0070062 extracellular exosome reference: PMID:20458337 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Myristoylated cytosolic small GTPase that cycles onto membranes. Cytosolic protein with lipid or membrane-protein binding ability; MISEV2018 (PMID:30637094) category 2a lists such proteins (ESCRT components, ALIX, annexins, HSPA8 and others) as expected EV contents. True, but not its core location. - gene: human/ARHGAP23 row_index: 7 term: GO:0070062 extracellular exosome reference: PMID:19199708 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic Rho GAP. Cytosolic protein (GO cytosol or cytoplasm annotation); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/ARHGEF18 row_index: 33 term: GO:0070062 extracellular exosome reference: PMID:18570454 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic/cortical Rho GEF. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/ARL6 row_index: 24 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Peripheral ciliary-membrane GTPase (urinary EVs carry ciliary proteins). Cytosolic protein with lipid or membrane-protein binding ability; MISEV2018 (PMID:30637094) category 2a lists such proteins (ESCRT components, ALIX, annexins, HSPA8 and others) as expected EV contents. True, but not its core location. - gene: human/ARL8A row_index: 29 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Peripheral late-endosome/lysosome GTPase. Cytosolic protein with lipid or membrane-protein binding ability; MISEV2018 (PMID:30637094) category 2a lists such proteins (ESCRT components, ALIX, annexins, HSPA8 and others) as expected EV contents. True, but not its core location. - gene: human/ARL8B row_index: 68 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Peripheral late-endosome/lysosome GTPase. Cytosolic protein with lipid or membrane-protein binding ability; MISEV2018 (PMID:30637094) category 2a lists such proteins (ESCRT components, ALIX, annexins, HSPA8 and others) as expected EV contents. True, but not its core location. - gene: human/ARMC9 row_index: 19 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: UNDECIDED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic ciliary basal-body protein. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/ARPC1B row_index: 33 term: GO:0070062 extracellular exosome reference: PMID:20458337 previous_action: UNDECIDED decision: KEEP_AS_NON_CORE changed: true basis: Arp2/3 complex subunit. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/ARSA row_index: 18 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: UNDECIDED decision: MARK_AS_OVER_ANNOTATED changed: true basis: Soluble lysosomal sulfatase. Secreted or lumenal protein (UniProt). MISEV2018 (PMID:30637094) category 5 allows such proteins onto EVs through specific or promiscuous (proteoglycan, lipid) receptors on the EV surface, but asks that their identification be backed by evidence of that association. A bulk EV proteome cannot separate surface binding from co-isolation, and this protein's annotations record no experimental proteoglycan, heparin or lipid binding, and its own review keeps no experimental cell-surface or plasma-membrane pool. - gene: human/ARSB row_index: 27 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: UNDECIDED decision: MARK_AS_OVER_ANNOTATED changed: true basis: Soluble lysosomal sulfatase. Secreted or lumenal protein (UniProt). MISEV2018 (PMID:30637094) category 5 allows such proteins onto EVs through specific or promiscuous (proteoglycan, lipid) receptors on the EV surface, but asks that their identification be backed by evidence of that association. A bulk EV proteome cannot separate surface binding from co-isolation, and this protein's annotations record no experimental proteoglycan, heparin or lipid binding, and its own review keeps no experimental cell-surface or plasma-membrane pool. - gene: human/ASS1 row_index: 69 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: UNDECIDED decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic urea-cycle enzyme. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/ATIC row_index: 41 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic purine-synthesis enzyme. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/ATIC row_index: 43 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic purine-synthesis enzyme. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/ATIC row_index: 44 term: GO:0070062 extracellular exosome reference: PMID:20458337 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic purine-synthesis enzyme. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/ATP1A1 row_index: 106 term: GO:0070062 extracellular exosome reference: PMID:12519789 previous_action: UNDECIDED decision: KEEP_AS_NON_CORE changed: true basis: Plasma-membrane Na+/K+-ATPase. Transmembrane/lipid-anchored protein of the plasma membrane or endosomal system (UniProt); such proteins are expected EV contents (MISEV2018 (PMID:30637094) category 1). True, but not its core location. - gene: human/ATP1A1 row_index: 107 term: GO:0070062 extracellular exosome reference: PMID:19199708 previous_action: UNDECIDED decision: KEEP_AS_NON_CORE changed: true basis: Plasma-membrane Na+/K+-ATPase. Transmembrane/lipid-anchored protein of the plasma membrane or endosomal system (UniProt); such proteins are expected EV contents (MISEV2018 (PMID:30637094) category 1). True, but not its core location. - gene: human/ATP1A1 row_index: 108 term: GO:0070062 extracellular exosome reference: PMID:20458337 previous_action: UNDECIDED decision: KEEP_AS_NON_CORE changed: true basis: Plasma-membrane Na+/K+-ATPase. Transmembrane/lipid-anchored protein of the plasma membrane or endosomal system (UniProt); such proteins are expected EV contents (MISEV2018 (PMID:30637094) category 1). True, but not its core location. - gene: human/ATP1A1 row_index: 118 term: GO:1903561 extracellular vesicle reference: PMID:24769233 previous_action: UNDECIDED decision: KEEP_AS_NON_CORE changed: true basis: Plasma-membrane Na+/K+-ATPase. Transmembrane/lipid-anchored protein of the plasma membrane or endosomal system (UniProt); such proteins are expected EV contents (MISEV2018 (PMID:30637094) category 1). True, but not its core location. - gene: human/ATP1A2 row_index: 121 term: GO:1903561 extracellular vesicle reference: PMID:24769233 previous_action: UNDECIDED decision: KEEP_AS_NON_CORE changed: true basis: Plasma-membrane Na+/K+-ATPase. Transmembrane/lipid-anchored protein of the plasma membrane or endosomal system (UniProt); such proteins are expected EV contents (MISEV2018 (PMID:30637094) category 1). True, but not its core location. - gene: human/ATP1A3 row_index: 72 term: GO:1903561 extracellular vesicle reference: PMID:24769233 previous_action: UNDECIDED decision: KEEP_AS_NON_CORE changed: true basis: Plasma-membrane Na+/K+-ATPase. Transmembrane/lipid-anchored protein of the plasma membrane or endosomal system (UniProt); such proteins are expected EV contents (MISEV2018 (PMID:30637094) category 1). True, but not its core location. - gene: human/ATP2B2 row_index: 39 term: GO:0070062 extracellular exosome reference: PMID:18570454 previous_action: UNDECIDED decision: KEEP_AS_NON_CORE changed: true basis: Plasma-membrane Ca2+-ATPase. Transmembrane/lipid-anchored protein of the plasma membrane or endosomal system (UniProt); such proteins are expected EV contents (MISEV2018 (PMID:30637094) category 1). True, but not its core location. - gene: human/ATP6AP1 row_index: 52 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: UNDECIDED decision: MARK_AS_OVER_ANNOTATED changed: true basis: UniProt places ATP6AP1 in ER and ER-Golgi intermediate compartment membranes. Mainly resident in an intracellular compartment that MISEV2018 (PMID:30637094) lists as not enriched in small EVs of plasma-membrane/endosomal origin (category 4), and its own review keeps no experimental cell-surface or plasma-membrane pool; an exosome annotation from a bulk EV proteome is not supported. - gene: human/ATP6AP2 row_index: 47 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: UNDECIDED decision: KEEP_AS_NON_CORE changed: true basis: Prorenin receptor / V-ATPase accessory protein, with UniProt lysosome-membrane and cell-surface locations. Transmembrane/lipid-anchored protein of the plasma membrane or endosomal system (UniProt); such proteins are expected EV contents (MISEV2018 (PMID:30637094) category 1). True, but not its core location. - gene: human/ATP6AP2 row_index: 53 term: GO:0070062 extracellular exosome reference: PMID:19199708 previous_action: UNDECIDED decision: KEEP_AS_NON_CORE changed: true basis: Prorenin receptor / V-ATPase accessory protein, with UniProt lysosome-membrane and cell-surface locations. Transmembrane/lipid-anchored protein of the plasma membrane or endosomal system (UniProt); such proteins are expected EV contents (MISEV2018 (PMID:30637094) category 1). True, but not its core location. - gene: human/ATP6V0A1 row_index: 31 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: V-ATPase V0 a1 subunit of endosomal/vesicle membranes. Transmembrane/lipid-anchored protein of the plasma membrane or endosomal system (UniProt membrane topology; compartment from GO); such proteins are expected EV contents (MISEV2018 (PMID:30637094) category 1). True, but not its core location. - gene: human/ATP6V0A1 row_index: 32 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: V-ATPase V0 a1 subunit of endosomal/vesicle membranes. Transmembrane/lipid-anchored protein of the plasma membrane or endosomal system (UniProt membrane topology; compartment from GO); such proteins are expected EV contents (MISEV2018 (PMID:30637094) category 1). True, but not its core location. - gene: human/ATP6V1A row_index: 54 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: V-ATPase V1 subunit, assembled on endosomal and apical membranes from the cytosol. Cytosolic protein with lipid or membrane-protein binding ability; MISEV2018 (PMID:30637094) category 2a lists such proteins (ESCRT components, ALIX, annexins, HSPA8 and others) as expected EV contents. True, but not its core location. - gene: human/ATP6V1A row_index: 55 term: GO:0070062 extracellular exosome reference: PMID:19199708 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: V-ATPase V1 subunit, assembled on endosomal and apical membranes from the cytosol. Cytosolic protein with lipid or membrane-protein binding ability; MISEV2018 (PMID:30637094) category 2a lists such proteins (ESCRT components, ALIX, annexins, HSPA8 and others) as expected EV contents. True, but not its core location. - gene: human/ATP6V1A row_index: 56 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: V-ATPase V1 subunit, assembled on endosomal and apical membranes from the cytosol. Cytosolic protein with lipid or membrane-protein binding ability; MISEV2018 (PMID:30637094) category 2a lists such proteins (ESCRT components, ALIX, annexins, HSPA8 and others) as expected EV contents. True, but not its core location. - gene: human/ATP6V1A row_index: 58 term: GO:0070062 extracellular exosome reference: PMID:20458337 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: V-ATPase V1 subunit, assembled on endosomal and apical membranes from the cytosol. Cytosolic protein with lipid or membrane-protein binding ability; MISEV2018 (PMID:30637094) category 2a lists such proteins (ESCRT components, ALIX, annexins, HSPA8 and others) as expected EV contents. True, but not its core location. - gene: human/ATP6V1B1 row_index: 32 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: UNDECIDED decision: KEEP_AS_NON_CORE changed: true basis: V-ATPase V1 subunit of the apical membrane of renal intercalated cells. Cytosolic protein with lipid or membrane-protein binding ability; MISEV2018 (PMID:30637094) category 2a lists such proteins (ESCRT components, ALIX, annexins, HSPA8 and others) as expected EV contents. True, but not its core location. - gene: human/ATP6V1B1 row_index: 33 term: GO:0070062 extracellular exosome reference: PMID:19199708 previous_action: UNDECIDED decision: KEEP_AS_NON_CORE changed: true basis: V-ATPase V1 subunit of the apical membrane of renal intercalated cells. Cytosolic protein with lipid or membrane-protein binding ability; MISEV2018 (PMID:30637094) category 2a lists such proteins (ESCRT components, ALIX, annexins, HSPA8 and others) as expected EV contents. True, but not its core location. - gene: human/ATP6V1B1 row_index: 34 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: UNDECIDED decision: KEEP_AS_NON_CORE changed: true basis: V-ATPase V1 subunit of the apical membrane of renal intercalated cells. Cytosolic protein with lipid or membrane-protein binding ability; MISEV2018 (PMID:30637094) category 2a lists such proteins (ESCRT components, ALIX, annexins, HSPA8 and others) as expected EV contents. True, but not its core location. - gene: human/ATP6V1B2 row_index: 30 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: V-ATPase V1 subunit. Cytosolic protein with lipid or membrane-protein binding ability; MISEV2018 (PMID:30637094) category 2a lists such proteins (ESCRT components, ALIX, annexins, HSPA8 and others) as expected EV contents. True, but not its core location. - gene: human/ATP6V1B2 row_index: 31 term: GO:0070062 extracellular exosome reference: PMID:19199708 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: V-ATPase V1 subunit. Cytosolic protein with lipid or membrane-protein binding ability; MISEV2018 (PMID:30637094) category 2a lists such proteins (ESCRT components, ALIX, annexins, HSPA8 and others) as expected EV contents. True, but not its core location. - gene: human/ATP6V1B2 row_index: 32 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: V-ATPase V1 subunit. Cytosolic protein with lipid or membrane-protein binding ability; MISEV2018 (PMID:30637094) category 2a lists such proteins (ESCRT components, ALIX, annexins, HSPA8 and others) as expected EV contents. True, but not its core location. - gene: human/ATP6V1C1 row_index: 16 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: V-ATPase V1 subunit. Cytosolic protein with lipid or membrane-protein binding ability; MISEV2018 (PMID:30637094) category 2a lists such proteins (ESCRT components, ALIX, annexins, HSPA8 and others) as expected EV contents. True, but not its core location. - gene: human/ATP6V1C2 row_index: 8 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: V-ATPase V1 subunit. Cytosolic protein with lipid or membrane-protein binding ability; MISEV2018 (PMID:30637094) category 2a lists such proteins (ESCRT components, ALIX, annexins, HSPA8 and others) as expected EV contents. True, but not its core location. - gene: human/ATP6V1D row_index: 41 term: GO:0070062 extracellular exosome reference: PMID:19199708 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: V-ATPase V1 subunit. Cytosolic protein with lipid or membrane-protein binding ability; MISEV2018 (PMID:30637094) category 2a lists such proteins (ESCRT components, ALIX, annexins, HSPA8 and others) as expected EV contents. True, but not its core location. - gene: human/ATP6V1D row_index: 42 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: V-ATPase V1 subunit. Cytosolic protein with lipid or membrane-protein binding ability; MISEV2018 (PMID:30637094) category 2a lists such proteins (ESCRT components, ALIX, annexins, HSPA8 and others) as expected EV contents. True, but not its core location. - gene: human/ATP6V1E1 row_index: 19 term: GO:0070062 extracellular exosome reference: PMID:19199708 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: V-ATPase V1 subunit. Cytosolic protein with lipid or membrane-protein binding ability; MISEV2018 (PMID:30637094) category 2a lists such proteins (ESCRT components, ALIX, annexins, HSPA8 and others) as expected EV contents. True, but not its core location. - gene: human/ATP6V1E1 row_index: 20 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: V-ATPase V1 subunit. Cytosolic protein with lipid or membrane-protein binding ability; MISEV2018 (PMID:30637094) category 2a lists such proteins (ESCRT components, ALIX, annexins, HSPA8 and others) as expected EV contents. True, but not its core location. - gene: human/ATP6V1F row_index: 28 term: GO:0070062 extracellular exosome reference: PMID:19199708 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: V-ATPase V1 subunit. Cytosolic protein with lipid or membrane-protein binding ability; MISEV2018 (PMID:30637094) category 2a lists such proteins (ESCRT components, ALIX, annexins, HSPA8 and others) as expected EV contents. True, but not its core location. - gene: human/ATP6V1F row_index: 29 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: V-ATPase V1 subunit. Cytosolic protein with lipid or membrane-protein binding ability; MISEV2018 (PMID:30637094) category 2a lists such proteins (ESCRT components, ALIX, annexins, HSPA8 and others) as expected EV contents. True, but not its core location. - gene: human/ATP6V1H row_index: 28 term: GO:0070062 extracellular exosome reference: PMID:19199708 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: V-ATPase V1 subunit. Cytosolic protein with lipid or membrane-protein binding ability; MISEV2018 (PMID:30637094) category 2a lists such proteins (ESCRT components, ALIX, annexins, HSPA8 and others) as expected EV contents. True, but not its core location. - gene: human/ATP6V1H row_index: 29 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: V-ATPase V1 subunit. Cytosolic protein with lipid or membrane-protein binding ability; MISEV2018 (PMID:30637094) category 2a lists such proteins (ESCRT components, ALIX, annexins, HSPA8 and others) as expected EV contents. True, but not its core location. - gene: human/AUP1 row_index: 39 term: GO:0070062 extracellular exosome reference: PMID:18570454 previous_action: MARK_AS_OVER_ANNOTATED decision: MARK_AS_OVER_ANNOTATED changed: false basis: ER-membrane and lipid-droplet protein (UniProt ECO:0000269). Mainly resident in an intracellular compartment that MISEV2018 (PMID:30637094) lists as not enriched in small EVs of plasma-membrane/endosomal origin (category 4), and its own review keeps no experimental cell-surface or plasma-membrane pool; an exosome annotation from a bulk EV proteome is not supported. - gene: human/BLVRB row_index: 23 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic reductase. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/BPGM row_index: 13 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic erythrocyte enzyme. Cytosolic protein (GO cytosol or cytoplasm annotation); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/BPTF row_index: 42 term: GO:0070062 extracellular exosome reference: PMID:18570454 previous_action: UNDECIDED decision: MARK_AS_OVER_ANNOTATED changed: true basis: Nuclear chromatin-remodelling subunit. Mainly resident in an intracellular compartment that MISEV2018 (PMID:30637094) lists as not enriched in small EVs of plasma-membrane/endosomal origin (category 4), and its own review keeps no experimental cell-surface or plasma-membrane pool; an exosome annotation from a bulk EV proteome is not supported. - gene: human/BTD row_index: 15 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: UNDECIDED decision: MARK_AS_OVER_ANNOTATED changed: true basis: Secreted biotinidase. Secreted or lumenal protein (UniProt). MISEV2018 (PMID:30637094) category 5 allows such proteins onto EVs through specific or promiscuous (proteoglycan, lipid) receptors on the EV surface, but asks that their identification be backed by evidence of that association. A bulk EV proteome cannot separate surface binding from co-isolation, and this protein's annotations record no experimental proteoglycan, heparin or lipid binding, and its own review keeps no experimental cell-surface or plasma-membrane pool. - gene: human/C1GALT1C1 row_index: 8 term: GO:0070062 extracellular exosome reference: PMID:19199708 previous_action: MARK_AS_OVER_ANNOTATED decision: MARK_AS_OVER_ANNOTATED changed: false basis: ER-membrane chaperone (Cosmc). Mainly resident in an intracellular compartment that MISEV2018 (PMID:30637094) lists as not enriched in small EVs of plasma-membrane/endosomal origin (category 4), and its own review keeps no experimental cell-surface or plasma-membrane pool; an exosome annotation from a bulk EV proteome is not supported. - gene: human/C1QB row_index: 56 term: GO:0072562 blood microparticle reference: PMID:22516433 previous_action: UNDECIDED decision: KEEP_AS_NON_CORE changed: true basis: Secreted complement C1q subunit with experimental phosphatidylserine binding (IDA). MISEV2018 (PMID:30637094) category 5 allows secreted proteins onto EVs through promiscuous (proteoglycan, lipid) receptors on the EV surface, so the detection is plausible. True, but not its core location. - gene: human/C3 row_index: 130 term: GO:0070062 extracellular exosome reference: PMID:21362503 previous_action: UNDECIDED decision: KEEP_AS_NON_CORE changed: true basis: Secreted complement C3; C3b binds covalently to cell surfaces through its exposed thioester, and its review accepts five cell-surface rows by EXP. Secreted or lumenal protein (UniProt) with an experimentally documented cell-surface or plasma-membrane pool that its own review keeps. MISEV2018 (PMID:30637094) category 5 allows such proteins onto EVs through specific or promiscuous receptors on the EV surface, and small EVs budding from the plasma membrane can carry a surface-bound pool, so the detection is plausible. True, but not its core location. - gene: human/C3 row_index: 131 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: UNDECIDED decision: KEEP_AS_NON_CORE changed: true basis: Secreted complement C3; C3b binds covalently to cell surfaces through its exposed thioester, and its review accepts five cell-surface rows by EXP. Secreted or lumenal protein (UniProt) with an experimentally documented cell-surface or plasma-membrane pool that its own review keeps. MISEV2018 (PMID:30637094) category 5 allows such proteins onto EVs through specific or promiscuous receptors on the EV surface, and small EVs budding from the plasma membrane can carry a surface-bound pool, so the detection is plausible. True, but not its core location. - gene: human/C3 row_index: 132 term: GO:0072562 blood microparticle reference: PMID:22516433 previous_action: UNDECIDED decision: KEEP_AS_NON_CORE changed: true basis: Secreted complement C3; C3b binds covalently to cell surfaces through its exposed thioester, and its review accepts five cell-surface rows by EXP. Secreted or lumenal protein (UniProt) with an experimentally documented cell-surface or plasma-membrane pool that its own review keeps. MISEV2018 (PMID:30637094) category 5 allows such proteins onto EVs through specific or promiscuous receptors on the EV surface, and small EVs budding from the plasma membrane can carry a surface-bound pool, so the detection is plausible. True, but not its core location. - gene: human/CACNA2D1 row_index: 29 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Plasma-membrane calcium-channel subunit. Transmembrane/lipid-anchored protein of the plasma membrane or endosomal system (UniProt); such proteins are expected EV contents (MISEV2018 (PMID:30637094) category 1). True, but not its core location. - gene: human/CACNA2D1 row_index: 30 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Plasma-membrane calcium-channel subunit. Transmembrane/lipid-anchored protein of the plasma membrane or endosomal system (UniProt); such proteins are expected EV contents (MISEV2018 (PMID:30637094) category 1). True, but not its core location. - gene: human/CACYBP row_index: 26 term: GO:0070062 extracellular exosome reference: PMID:20458337 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic/nuclear calcyclin-binding protein. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/CAD row_index: 69 term: GO:0070062 extracellular exosome reference: PMID:20458337 previous_action: UNDECIDED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic pyrimidine-synthesis enzyme. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/CAND1 row_index: 35 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic cullin-exchange factor. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/CAND1 row_index: 38 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic cullin-exchange factor. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/CAND1 row_index: 39 term: GO:0070062 extracellular exosome reference: PMID:20458337 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic cullin-exchange factor. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/CANT1 row_index: 32 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: UNDECIDED decision: MARK_AS_OVER_ANNOTATED changed: true basis: ER/Golgi-membrane nucleotidase. Mainly resident in an intracellular compartment that MISEV2018 (PMID:30637094) lists as not enriched in small EVs of plasma-membrane/endosomal origin (category 4), and its own review keeps no experimental cell-surface or plasma-membrane pool; an exosome annotation from a bulk EV proteome is not supported. - gene: human/CANX row_index: 58 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: MARK_AS_OVER_ANNOTATED changed: false basis: ER-membrane chaperone; calnexin is a commonly used ER (non-small-EV) marker. Mainly resident in an intracellular compartment that MISEV2018 (PMID:30637094) lists as not enriched in small EVs of plasma-membrane/endosomal origin (category 4), and its own review keeps no experimental cell-surface or plasma-membrane pool; an exosome annotation from a bulk EV proteome is not supported. - gene: human/CAPN5 row_index: 12 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: UNDECIDED decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic calpain. Cytosolic protein (GO cytosol or cytoplasm annotation); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/CAPN5 row_index: 13 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: UNDECIDED decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic calpain. Cytosolic protein (GO cytosol or cytoplasm annotation); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/CARD11 row_index: 87 term: GO:0070062 extracellular exosome reference: PMID:18570454 previous_action: UNDECIDED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic/membrane-raft signalling scaffold. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/CC2D1A row_index: 18 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: UNDECIDED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic protein that binds ESCRT-III. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/CC2D1A row_index: 19 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: UNDECIDED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic protein that binds ESCRT-III. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/CD2AP row_index: 60 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic adaptor linking membrane proteins to actin. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. superseded_by: ai4curation/ai-gene-review#4287 re-reviewed both rows (UNDECIDED pending the source tables) - gene: human/CD2AP row_index: 61 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic adaptor linking membrane proteins to actin. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. superseded_by: ai4curation/ai-gene-review#4287 re-reviewed both rows (UNDECIDED pending the source tables) - gene: human/CHMP3 row_index: 96 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: ACCEPT decision: KEEP_AS_NON_CORE changed: true basis: ESCRT-III subunit acting on the cytosolic face of endosomes; its presence in EVs follows from ILV formation, but the exosome is a destination, not where it acts. Cytosolic protein with lipid or membrane-protein binding ability; MISEV2018 (PMID:30637094) category 2a lists such proteins (ESCRT components, ALIX, annexins, HSPA8 and others) as expected EV contents. True, but not its core location. - gene: human/CLU row_index: 112 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: ACCEPT decision: KEEP_AS_NON_CORE changed: true basis: 'Secreted clusterin (UniProt isoform 1: secreted), with receptor-mediated cell-surface and plasma-membrane pools by IDA (PMID:17567961, PMID:30333625). The earlier ACCEPT rested on its being secreted, which does not make the exosome its core location. Applies to the plasma microvesicle row too. Secreted or lumenal protein (UniProt) with an experimentally documented cell-surface or plasma-membrane pool that its own review keeps. MISEV2018 (PMID:30637094) category 5 allows such proteins onto EVs through specific or promiscuous receptors on the EV surface, and small EVs budding from the plasma membrane can carry a surface-bound pool, so the detection is plausible. True, but not its core location.' - gene: human/CLU row_index: 130 term: GO:0072562 blood microparticle reference: PMID:22516433 previous_action: ACCEPT decision: KEEP_AS_NON_CORE changed: true basis: 'Secreted clusterin (UniProt isoform 1: secreted), with receptor-mediated cell-surface and plasma-membrane pools by IDA (PMID:17567961, PMID:30333625). The earlier ACCEPT rested on its being secreted, which does not make the exosome its core location. Applies to the plasma microvesicle row too. Secreted or lumenal protein (UniProt) with an experimentally documented cell-surface or plasma-membrane pool that its own review keeps. MISEV2018 (PMID:30637094) category 5 allows such proteins onto EVs through specific or promiscuous receptors on the EV surface, and small EVs budding from the plasma membrane can carry a surface-bound pool, so the detection is plausible. True, but not its core location.' - gene: human/CLU row_index: 131 term: GO:0070062 extracellular exosome reference: PMID:19199708 previous_action: ACCEPT decision: KEEP_AS_NON_CORE changed: true basis: 'Secreted clusterin (UniProt isoform 1: secreted), with receptor-mediated cell-surface and plasma-membrane pools by IDA (PMID:17567961, PMID:30333625). The earlier ACCEPT rested on its being secreted, which does not make the exosome its core location. Applies to the plasma microvesicle row too. Secreted or lumenal protein (UniProt) with an experimentally documented cell-surface or plasma-membrane pool that its own review keeps. MISEV2018 (PMID:30637094) category 5 allows such proteins onto EVs through specific or promiscuous receptors on the EV surface, and small EVs budding from the plasma membrane can carry a surface-bound pool, so the detection is plausible. True, but not its core location.' - gene: human/CLU row_index: 132 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: ACCEPT decision: KEEP_AS_NON_CORE changed: true basis: 'Secreted clusterin (UniProt isoform 1: secreted), with receptor-mediated cell-surface and plasma-membrane pools by IDA (PMID:17567961, PMID:30333625). The earlier ACCEPT rested on its being secreted, which does not make the exosome its core location. Applies to the plasma microvesicle row too. Secreted or lumenal protein (UniProt) with an experimentally documented cell-surface or plasma-membrane pool that its own review keeps. MISEV2018 (PMID:30637094) category 5 allows such proteins onto EVs through specific or promiscuous receptors on the EV surface, and small EVs budding from the plasma membrane can carry a surface-bound pool, so the detection is plausible. True, but not its core location.' - gene: human/CR1 row_index: 42 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Plasma-membrane complement receptor (podocyte/erythrocyte). Transmembrane/lipid-anchored protein of the plasma membrane or endosomal system (UniProt membrane topology; compartment from GO); such proteins are expected EV contents (MISEV2018 (PMID:30637094) category 1). True, but not its core location. - gene: human/CR1 row_index: 43 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Plasma-membrane complement receptor (podocyte/erythrocyte). Transmembrane/lipid-anchored protein of the plasma membrane or endosomal system (UniProt membrane topology; compartment from GO); such proteins are expected EV contents (MISEV2018 (PMID:30637094) category 1). True, but not its core location. - gene: human/CRYAB row_index: 87 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: ACCEPT decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic small heat-shock protein. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/CRYAB row_index: 88 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: ACCEPT decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic small heat-shock protein. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/CSNK2B row_index: 70 term: GO:0070062 extracellular exosome reference: PMID:20458337 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: CK2 regulatory subunit (cytoplasmic and nuclear). Cytosolic protein (GO cytosol or cytoplasm annotation); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/CTH row_index: 33 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic enzyme. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/CTNNB1 row_index: 671 term: GO:0070062 extracellular exosome reference: PMID:19199708 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic/junctional beta-catenin. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/CUL3 row_index: 171 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cullin scaffold with a cytoplasmic pool. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/DDB1 row_index: 158 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic/nuclear CRL4 adaptor. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/DNAJC7 row_index: 22 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic co-chaperone. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/DUT row_index: 34 term: GO:0070062 extracellular exosome reference: PMID:20458337 previous_action: MARK_AS_OVER_ANNOTATED decision: MARK_AS_OVER_ANNOTATED changed: false basis: Nuclear and mitochondrial dUTPase isoforms (UniProt). Mainly resident in an intracellular compartment that MISEV2018 (PMID:30637094) lists as not enriched in small EVs of plasma-membrane/endosomal origin (category 4), and its own review keeps no experimental cell-surface or plasma-membrane pool; an exosome annotation from a bulk EV proteome is not supported. - gene: human/DYNC1H1 row_index: 148 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic dynein heavy chain. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/DYNC1H1 row_index: 149 term: GO:0070062 extracellular exosome reference: PMID:19199708 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic dynein heavy chain. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/DYNC1H1 row_index: 150 term: GO:0070062 extracellular exosome reference: PMID:20458337 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic dynein heavy chain. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/DYNC1H1 row_index: 151 term: GO:0070062 extracellular exosome reference: PMID:21362503 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic dynein heavy chain. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/DYNC1H1 row_index: 152 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic dynein heavy chain. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/ENO3 row_index: 21 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic enolase. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/ENPP4 row_index: 10 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: ACCEPT decision: KEEP_AS_NON_CORE changed: true basis: Plasma-membrane ectoenzyme; the earlier ACCEPT asserted activity in exosomes without evidence. Transmembrane/lipid-anchored protein of the plasma membrane or endosomal system (UniProt); such proteins are expected EV contents (MISEV2018 (PMID:30637094) category 1). True, but not its core location. - gene: human/FASN row_index: 59 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic fatty-acid synthase. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/FASN row_index: 63 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic fatty-acid synthase. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/FASN row_index: 64 term: GO:0070062 extracellular exosome reference: PMID:20458337 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic fatty-acid synthase. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/FTCD row_index: 30 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic enzyme. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/GAA row_index: 31 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: MARK_AS_OVER_ANNOTATED changed: false basis: Soluble lysosomal alpha-glucosidase. Secreted or lumenal protein (UniProt). MISEV2018 (PMID:30637094) category 5 allows such proteins onto EVs through specific or promiscuous (proteoglycan, lipid) receptors on the EV surface, but asks that their identification be backed by evidence of that association. A bulk EV proteome cannot separate surface binding from co-isolation, and this protein's annotations record no experimental proteoglycan, heparin or lipid binding, and its own review keeps no experimental cell-surface or plasma-membrane pool. - gene: human/GAA row_index: 35 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: MARK_AS_OVER_ANNOTATED changed: false basis: Soluble lysosomal alpha-glucosidase. Secreted or lumenal protein (UniProt). MISEV2018 (PMID:30637094) category 5 allows such proteins onto EVs through specific or promiscuous (proteoglycan, lipid) receptors on the EV surface, but asks that their identification be backed by evidence of that association. A bulk EV proteome cannot separate surface binding from co-isolation, and this protein's annotations record no experimental proteoglycan, heparin or lipid binding, and its own review keeps no experimental cell-surface or plasma-membrane pool. - gene: human/GAPDH row_index: 65 term: GO:0070062 extracellular exosome reference: PMID:12519789 previous_action: ACCEPT decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic glycolytic enzyme found in many EV proteomes; the exosome is a destination, not GAPDH's core location, so the earlier ACCEPT is downgraded. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/GAPDH row_index: 66 term: GO:0070062 extracellular exosome reference: PMID:11487543 previous_action: ACCEPT decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic glycolytic enzyme found in many EV proteomes; the exosome is a destination, not GAPDH's core location, so the earlier ACCEPT is downgraded. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/GAPDH row_index: 68 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: ACCEPT decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic glycolytic enzyme found in many EV proteomes; the exosome is a destination, not GAPDH's core location, so the earlier ACCEPT is downgraded. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/GAPDH row_index: 74 term: GO:0070062 extracellular exosome reference: PMID:19199708 previous_action: ACCEPT decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic glycolytic enzyme found in many EV proteomes; the exosome is a destination, not GAPDH's core location, so the earlier ACCEPT is downgraded. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/GAPDH row_index: 75 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: ACCEPT decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic glycolytic enzyme found in many EV proteomes; the exosome is a destination, not GAPDH's core location, so the earlier ACCEPT is downgraded. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/GAPDH row_index: 76 term: GO:0070062 extracellular exosome reference: PMID:20458337 previous_action: ACCEPT decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic glycolytic enzyme found in many EV proteomes; the exosome is a destination, not GAPDH's core location, so the earlier ACCEPT is downgraded. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/GAPDH row_index: 83 term: GO:0070062 extracellular exosome reference: PMID:21362503 previous_action: ACCEPT decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic glycolytic enzyme found in many EV proteomes; the exosome is a destination, not GAPDH's core location, so the earlier ACCEPT is downgraded. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/GART row_index: 38 term: GO:0070062 extracellular exosome reference: PMID:20458337 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic purine-synthesis enzyme. Cytosolic protein (GO cytosol or cytoplasm annotation); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/GATM row_index: 21 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: MARK_AS_OVER_ANNOTATED changed: false basis: Mitochondrial intermembrane-space enzyme. Mainly resident in an intracellular compartment that MISEV2018 (PMID:30637094) lists as not enriched in small EVs of plasma-membrane/endosomal origin (category 4), and its own review keeps no experimental cell-surface or plasma-membrane pool; an exosome annotation from a bulk EV proteome is not supported. - gene: human/GBE1 row_index: 18 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic glycogen-branching enzyme. Cytosolic protein (GO cytosol or cytoplasm annotation); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/GBE1 row_index: 19 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic glycogen-branching enzyme. Cytosolic protein (GO cytosol or cytoplasm annotation); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/GGCT row_index: 2 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic enzyme. Cytosolic protein (GO cytosol or cytoplasm annotation); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/GLUL row_index: 36 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic glutamine synthetase. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/GPC4 row_index: 25 term: GO:0070062 extracellular exosome reference: PMID:19199708 previous_action: ACCEPT decision: KEEP_AS_NON_CORE changed: true basis: GPI-anchored plasma-membrane glypican. Transmembrane/lipid-anchored protein of the plasma membrane or endosomal system (UniProt); such proteins are expected EV contents (MISEV2018 (PMID:30637094) category 1). True, but not its core location. - gene: human/GPD1 row_index: 15 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic enzyme. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/GPX4 row_index: 53 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: UNDECIDED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic isoform of the peroxidase. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/GRHPR row_index: 23 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic reductase. Cytosolic protein (GO cytosol or cytoplasm annotation); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/GRHPR row_index: 24 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic reductase. Cytosolic protein (GO cytosol or cytoplasm annotation); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/GRHPR row_index: 25 term: GO:0070062 extracellular exosome reference: PMID:20458337 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic reductase. Cytosolic protein (GO cytosol or cytoplasm annotation); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/GRID1 row_index: 48 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: MARK_AS_OVER_ANNOTATED changed: false basis: Postsynaptic glutamate-receptor delta subunit whose expression is essentially restricted to the CNS; detection in urinary exosomes is implausible for the native protein and more likely a peptide misassignment. - gene: human/HBS1L row_index: 29 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic GTPase. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/HEXB row_index: 62 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: MARK_AS_OVER_ANNOTATED changed: false basis: Soluble lysosomal hexosaminidase. Secreted or lumenal protein (UniProt). MISEV2018 (PMID:30637094) category 5 allows such proteins onto EVs through specific or promiscuous (proteoglycan, lipid) receptors on the EV surface, but asks that their identification be backed by evidence of that association. A bulk EV proteome cannot separate surface binding from co-isolation, and this protein's annotations record no experimental proteoglycan, heparin or lipid binding, and its own review keeps no experimental cell-surface or plasma-membrane pool. - gene: human/HSPA1A row_index: 103 term: GO:0031982 vesicle reference: PMID:19190083 previous_action: ACCEPT decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic Hsp70; Hsp70 is a classic category 2 EV protein. Expected EV cargo, not a core location, so the earlier ACCEPT is downgraded. Applies to the plasma microvesicle row too. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/HSPA1A row_index: 107 term: GO:0070062 extracellular exosome reference: PMID:19199708 previous_action: ACCEPT decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic Hsp70; Hsp70 is a classic category 2 EV protein. Expected EV cargo, not a core location, so the earlier ACCEPT is downgraded. Applies to the plasma microvesicle row too. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/HSPA1A row_index: 108 term: GO:0070062 extracellular exosome reference: PMID:20458337 previous_action: ACCEPT decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic Hsp70; Hsp70 is a classic category 2 EV protein. Expected EV cargo, not a core location, so the earlier ACCEPT is downgraded. Applies to the plasma microvesicle row too. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/HSPA1A row_index: 109 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: ACCEPT decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic Hsp70; Hsp70 is a classic category 2 EV protein. Expected EV cargo, not a core location, so the earlier ACCEPT is downgraded. Applies to the plasma microvesicle row too. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/HSPA1A row_index: 179 term: GO:0072562 blood microparticle reference: PMID:22516433 previous_action: ACCEPT decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic Hsp70; Hsp70 is a classic category 2 EV protein. Expected EV cargo, not a core location, so the earlier ACCEPT is downgraded. Applies to the plasma microvesicle row too. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/IMPDH2 row_index: 29 term: GO:0070062 extracellular exosome reference: PMID:20458337 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic enzyme. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/LDHA row_index: 36 term: GO:0070062 extracellular exosome reference: PMID:11487543 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic lactate dehydrogenase. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/LDHA row_index: 37 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic lactate dehydrogenase. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/LDHA row_index: 40 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic lactate dehydrogenase. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/LDHA row_index: 41 term: GO:0070062 extracellular exosome reference: PMID:20458337 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic lactate dehydrogenase. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/LDHB row_index: 40 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic lactate dehydrogenase. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/LDHB row_index: 42 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic lactate dehydrogenase. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/LDHB row_index: 43 term: GO:0070062 extracellular exosome reference: PMID:20458337 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic lactate dehydrogenase. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/LMAN1 row_index: 47 term: GO:0070062 extracellular exosome reference: PMID:19199708 previous_action: MARK_AS_OVER_ANNOTATED decision: MARK_AS_OVER_ANNOTATED changed: false basis: ERGIC/Golgi membrane lectin. Mainly resident in an intracellular compartment that MISEV2018 (PMID:30637094) lists as not enriched in small EVs of plasma-membrane/endosomal origin (category 4), and its own review keeps no experimental cell-surface or plasma-membrane pool; an exosome annotation from a bulk EV proteome is not supported. - gene: human/LMAN2 row_index: 12 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: ERGIC/Golgi membrane lectin; cell-surface and plasma-membrane pool by IDA (PMID:22016386). Mainly resident in an intracellular compartment that MISEV2018 (PMID:30637094) lists as not enriched in small EVs (category 4), but with an experimentally documented cell-surface or plasma-membrane pool that its own review keeps; small EVs budding from the plasma membrane can carry that pool, so the detection is plausible. True, but not its core location. - gene: human/LMAN2 row_index: 24 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: ERGIC/Golgi membrane lectin; cell-surface and plasma-membrane pool by IDA (PMID:22016386). Mainly resident in an intracellular compartment that MISEV2018 (PMID:30637094) lists as not enriched in small EVs (category 4), but with an experimentally documented cell-surface or plasma-membrane pool that its own review keeps; small EVs budding from the plasma membrane can carry that pool, so the detection is plausible. True, but not its core location. - gene: human/MDH1 row_index: 24 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic malate dehydrogenase. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/MDH1 row_index: 26 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic malate dehydrogenase. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/MDH2 row_index: 33 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: MARK_AS_OVER_ANNOTATED changed: false basis: Mitochondrial matrix malate dehydrogenase. Mainly resident in an intracellular compartment that MISEV2018 (PMID:30637094) lists as not enriched in small EVs of plasma-membrane/endosomal origin (category 4), and its own review keeps no experimental cell-surface or plasma-membrane pool; an exosome annotation from a bulk EV proteome is not supported. - gene: human/MDH2 row_index: 38 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: MARK_AS_OVER_ANNOTATED changed: false basis: Mitochondrial matrix malate dehydrogenase. Mainly resident in an intracellular compartment that MISEV2018 (PMID:30637094) lists as not enriched in small EVs of plasma-membrane/endosomal origin (category 4), and its own review keeps no experimental cell-surface or plasma-membrane pool; an exosome annotation from a bulk EV proteome is not supported. - gene: human/MDH2 row_index: 39 term: GO:0070062 extracellular exosome reference: PMID:20458337 previous_action: MARK_AS_OVER_ANNOTATED decision: MARK_AS_OVER_ANNOTATED changed: false basis: Mitochondrial matrix malate dehydrogenase. Mainly resident in an intracellular compartment that MISEV2018 (PMID:30637094) lists as not enriched in small EVs of plasma-membrane/endosomal origin (category 4), and its own review keeps no experimental cell-surface or plasma-membrane pool; an exosome annotation from a bulk EV proteome is not supported. - gene: human/MGAT1 row_index: 22 term: GO:1903561 extracellular vesicle reference: PMID:24769233 previous_action: MARK_AS_OVER_ANNOTATED decision: MARK_AS_OVER_ANNOTATED changed: false basis: Golgi-membrane glycosyltransferase. Mainly resident in an intracellular compartment that MISEV2018 (PMID:30637094) lists as not enriched in small EVs of plasma-membrane/endosomal origin (category 4), and its own review keeps no experimental cell-surface or plasma-membrane pool; an exosome annotation from a bulk EV proteome is not supported. - gene: human/MGAT1 row_index: 23 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: MARK_AS_OVER_ANNOTATED changed: false basis: Golgi-membrane glycosyltransferase. Mainly resident in an intracellular compartment that MISEV2018 (PMID:30637094) lists as not enriched in small EVs of plasma-membrane/endosomal origin (category 4), and its own review keeps no experimental cell-surface or plasma-membrane pool; an exosome annotation from a bulk EV proteome is not supported. - gene: human/MGAT1 row_index: 25 term: GO:0070062 extracellular exosome reference: PMID:19199708 previous_action: MARK_AS_OVER_ANNOTATED decision: MARK_AS_OVER_ANNOTATED changed: false basis: Golgi-membrane glycosyltransferase. Mainly resident in an intracellular compartment that MISEV2018 (PMID:30637094) lists as not enriched in small EVs of plasma-membrane/endosomal origin (category 4), and its own review keeps no experimental cell-surface or plasma-membrane pool; an exosome annotation from a bulk EV proteome is not supported. - gene: human/MYH10 row_index: 63 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Non-muscle myosin II. Cytosolic protein (GO cytosol or cytoplasm annotation); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/MYH9 row_index: 148 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Non-muscle myosin II. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/MYH9 row_index: 149 term: GO:0070062 extracellular exosome reference: PMID:19199708 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Non-muscle myosin II. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/MYH9 row_index: 150 term: GO:0070062 extracellular exosome reference: PMID:20458337 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Non-muscle myosin II. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/MYH9 row_index: 151 term: GO:0070062 extracellular exosome reference: PMID:21362503 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Non-muscle myosin II. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/MYH9 row_index: 152 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Non-muscle myosin II. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/NAGK row_index: 21 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic kinase. Cytosolic protein (GO cytosol or cytoplasm annotation); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/NANS row_index: 11 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic sialic-acid synthase. Cytosolic protein (GO cytosol or cytoplasm annotation); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/NEU1 row_index: 22 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Lysosomal sialidase (lysosome lumen/membrane), with a plasma-membrane pool by IDA (PMID:27917893). Secreted or lumenal protein (UniProt) with an experimentally documented cell-surface or plasma-membrane pool that its own review keeps. MISEV2018 (PMID:30637094) category 5 allows such proteins onto EVs through specific or promiscuous receptors on the EV surface, and small EVs budding from the plasma membrane can carry a surface-bound pool, so the detection is plausible. True, but not its core location. - gene: human/NEU1 row_index: 26 term: GO:0070062 extracellular exosome reference: PMID:19199708 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Lysosomal sialidase (lysosome lumen/membrane), with a plasma-membrane pool by IDA (PMID:27917893). Secreted or lumenal protein (UniProt) with an experimentally documented cell-surface or plasma-membrane pool that its own review keeps. MISEV2018 (PMID:30637094) category 5 allows such proteins onto EVs through specific or promiscuous receptors on the EV surface, and small EVs budding from the plasma membrane can carry a surface-bound pool, so the detection is plausible. True, but not its core location. - gene: human/NRAS row_index: 155 term: GO:0070062 extracellular exosome reference: PMID:20458337 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Lipid-anchored plasma-membrane GTPase (cytoplasmic face). Transmembrane/lipid-anchored protein of the plasma membrane or endosomal system (UniProt); such proteins are expected EV contents (MISEV2018 (PMID:30637094) category 1). True, but not its core location. - gene: human/PAICS row_index: 36 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic purine-synthesis enzyme. Cytosolic protein (GO cytosol or cytoplasm annotation); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/PAICS row_index: 38 term: GO:0070062 extracellular exosome reference: PMID:20458337 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic purine-synthesis enzyme. Cytosolic protein (GO cytosol or cytoplasm annotation); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/PARK7 row_index: 185 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: ACCEPT decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic DJ-1; its EV presence matters as a biomarker, not as its site of action, so the earlier ACCEPT is downgraded. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/PARK7 row_index: 221 term: GO:0070062 extracellular exosome reference: PMID:19199708 previous_action: ACCEPT decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic DJ-1; its EV presence matters as a biomarker, not as its site of action, so the earlier ACCEPT is downgraded. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/PARK7 row_index: 222 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: ACCEPT decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic DJ-1; its EV presence matters as a biomarker, not as its site of action, so the earlier ACCEPT is downgraded. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/PARK7 row_index: 223 term: GO:0070062 extracellular exosome reference: PMID:20458337 previous_action: ACCEPT decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic DJ-1; its EV presence matters as a biomarker, not as its site of action, so the earlier ACCEPT is downgraded. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/PDCD6IP row_index: 67 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: ACCEPT decision: ACCEPT changed: false basis: 'ALIX itself works in EV biogenesis: UniProt records that it sorts multivesicular-body cargo into intralumenal vesicles and regulates exosome biogenesis with SDC1/4 and SDCBP (PubMed:22660413). This is a protein-specific call, not a MISEV category one: MISEV2018 (PMID:30637094) lists ALIX in category 2a together with TSG101 and the CHMPs.' - gene: human/PDCD6IP row_index: 76 term: GO:0070062 extracellular exosome reference: PMID:19199708 previous_action: ACCEPT decision: ACCEPT changed: false basis: 'ALIX itself works in EV biogenesis: UniProt records that it sorts multivesicular-body cargo into intralumenal vesicles and regulates exosome biogenesis with SDC1/4 and SDCBP (PubMed:22660413). This is a protein-specific call, not a MISEV category one: MISEV2018 (PMID:30637094) lists ALIX in category 2a together with TSG101 and the CHMPs.' - gene: human/PDCD6IP row_index: 77 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: ACCEPT decision: ACCEPT changed: false basis: 'ALIX itself works in EV biogenesis: UniProt records that it sorts multivesicular-body cargo into intralumenal vesicles and regulates exosome biogenesis with SDC1/4 and SDCBP (PubMed:22660413). This is a protein-specific call, not a MISEV category one: MISEV2018 (PMID:30637094) lists ALIX in category 2a together with TSG101 and the CHMPs.' - gene: human/PDXK row_index: 20 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic kinase. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/PEX1 row_index: 36 term: GO:0070062 extracellular exosome reference: PMID:18570454 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic AAA ATPase recruited to peroxisomes (UniProt cytosol, ECO:0000269). Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/PFAS row_index: 20 term: GO:0070062 extracellular exosome reference: PMID:20458337 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic purine-synthesis enzyme. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/PGAM2 row_index: 25 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic glycolytic enzyme. Cytosolic protein (GO cytosol or cytoplasm annotation); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/PGD row_index: 20 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic pentose-phosphate enzyme. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/PGD row_index: 22 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic pentose-phosphate enzyme. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/PGD row_index: 23 term: GO:0070062 extracellular exosome reference: PMID:20458337 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic pentose-phosphate enzyme. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/PGLS row_index: 16 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic pentose-phosphate enzyme. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/PGLS row_index: 17 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic pentose-phosphate enzyme. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/PLCG2 row_index: 121 term: GO:0070062 extracellular exosome reference: PMID:20458337 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic/membrane-raft phospholipase. Cytosolic protein with lipid or membrane-protein binding ability; MISEV2018 (PMID:30637094) category 2a lists such proteins (ESCRT components, ALIX, annexins, HSPA8 and others) as expected EV contents. True, but not its core location. - gene: human/PMVK row_index: 18 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic kinase. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/PPP2R1A row_index: 92 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic PP2A scaffold. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/PTPN6 row_index: 112 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic tyrosine phosphatase. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/PTPN6 row_index: 113 term: GO:0070062 extracellular exosome reference: PMID:20458337 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic tyrosine phosphatase. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/PYGL row_index: 22 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic glycogen phosphorylase. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/PYGM row_index: 19 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic glycogen phosphorylase. Cytosolic protein (GO cytosol or cytoplasm annotation); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/RAB1B row_index: 63 term: GO:0070062 extracellular exosome reference: PMID:20458337 previous_action: MARK_AS_OVER_ANNOTATED decision: MARK_AS_OVER_ANNOTATED changed: false basis: Prenylated GTPase of the ER-to-Golgi pathway (early secretory compartments). Mainly resident in an intracellular compartment that MISEV2018 (PMID:30637094) lists as not enriched in small EVs of plasma-membrane/endosomal origin (category 4), and its own review keeps no experimental cell-surface or plasma-membrane pool; an exosome annotation from a bulk EV proteome is not supported. - gene: human/RACK1 row_index: 94 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: MARK_AS_OVER_ANNOTATED changed: false basis: RACK1 is a core 40S ribosomal subunit protein as well as a signalling scaffold. Ribosomal protein; MISEV2018 (PMID:30637094) category 3b lists ribosomal proteins among the protein and protein/nucleic-acid aggregates that co-isolate with EVs, so detection does not establish EV localization. - gene: human/RPE row_index: 14 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic epimerase. Cytosolic protein (GO cytosol or cytoplasm annotation); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/RPS3 row_index: 143 term: GO:0070062 extracellular exosome reference: PMID:20458337 previous_action: MARK_AS_OVER_ANNOTATED decision: MARK_AS_OVER_ANNOTATED changed: false basis: 40S ribosomal protein S3. Ribosomal protein; MISEV2018 (PMID:30637094) category 3b lists ribosomal proteins among the protein and protein/nucleic-acid aggregates that co-isolate with EVs, so detection does not establish EV localization. - gene: human/SCAMP3 row_index: 6 term: GO:0070062 extracellular exosome reference: PMID:20458337 previous_action: ACCEPT decision: KEEP_AS_NON_CORE changed: true basis: Endosomal/post-Golgi membrane protein. The earlier ACCEPT said SCAMP3 is required for EV biogenesis, but the cited work (PMID:19158374, PMID:23418353) shows it regulates ESCRT-dependent MVB sorting of EGFR, not EV biogenesis. Transmembrane/lipid-anchored protein of the plasma membrane or endosomal system (UniProt membrane topology; compartment from GO); such proteins are expected EV contents (MISEV2018 (PMID:30637094) category 1). True, but not its core location. - gene: human/SCGB1A1 row_index: 35 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: ACCEPT decision: MARK_AS_OVER_ANNOTATED changed: true basis: Secreted uteroglobin (UniProt). MISEV2018 (PMID:30637094) category 5 allows such proteins onto EVs through specific or promiscuous (proteoglycan, lipid) receptors on the EV surface, but asks that their identification be backed by evidence of that association. A bulk EV proteome cannot separate surface binding from co-isolation. SCGB1A1 does bind phosphatidylcholine and phosphatidylinositol (UniProt), but as ligands held in the central hydrophobic cavity of its antiparallel homodimer (PubMed:7664082); no binding to membrane surfaces is recorded, so this is ligand sequestration, not the EV-surface route. The earlier ACCEPT rested on prostate expression, which does not establish EV association. - gene: human/SERINC5 row_index: 16 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: UNDECIDED decision: KEEP_AS_NON_CORE changed: true basis: Plasma-membrane multipass protein. Transmembrane/lipid-anchored protein of the plasma membrane or endosomal system (UniProt); such proteins are expected EV contents (MISEV2018 (PMID:30637094) category 1). True, but not its core location. - gene: human/SGSH row_index: 18 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: MARK_AS_OVER_ANNOTATED changed: false basis: Soluble lysosomal sulfamidase. Secreted or lumenal protein (UniProt). MISEV2018 (PMID:30637094) category 5 allows such proteins onto EVs through specific or promiscuous (proteoglycan, lipid) receptors on the EV surface, but asks that their identification be backed by evidence of that association. A bulk EV proteome cannot separate surface binding from co-isolation, and this protein's annotations record no experimental proteoglycan, heparin or lipid binding, and its own review keeps no experimental cell-surface or plasma-membrane pool. - gene: human/SHMT1 row_index: 76 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic serine hydroxymethyltransferase. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/SHMT1 row_index: 77 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic serine hydroxymethyltransferase. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/SHMT2 row_index: 77 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: MARK_AS_OVER_ANNOTATED changed: false basis: Mitochondrial serine hydroxymethyltransferase. Mainly resident in an intracellular compartment that MISEV2018 (PMID:30637094) lists as not enriched in small EVs of plasma-membrane/endosomal origin (category 4), and its own review keeps no experimental cell-surface or plasma-membrane pool; an exosome annotation from a bulk EV proteome is not supported. - gene: human/SHMT2 row_index: 78 term: GO:0070062 extracellular exosome reference: PMID:20458337 previous_action: MARK_AS_OVER_ANNOTATED decision: MARK_AS_OVER_ANNOTATED changed: false basis: Mitochondrial serine hydroxymethyltransferase. Mainly resident in an intracellular compartment that MISEV2018 (PMID:30637094) lists as not enriched in small EVs of plasma-membrane/endosomal origin (category 4), and its own review keeps no experimental cell-surface or plasma-membrane pool; an exosome annotation from a bulk EV proteome is not supported. - gene: human/SLC22A5 row_index: 69 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Apical plasma-membrane carnitine transporter. Transmembrane/lipid-anchored protein of the plasma membrane or endosomal system (UniProt); such proteins are expected EV contents (MISEV2018 (PMID:30637094) category 1). True, but not its core location. - gene: human/SLC25A1 row_index: 18 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: MARK_AS_OVER_ANNOTATED changed: false basis: Mitochondrial inner-membrane carrier. Mainly resident in an intracellular compartment that MISEV2018 (PMID:30637094) lists as not enriched in small EVs of plasma-membrane/endosomal origin (category 4), and its own review keeps no experimental cell-surface or plasma-membrane pool; an exosome annotation from a bulk EV proteome is not supported. - gene: human/SLC25A3 row_index: 16 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: MARK_AS_OVER_ANNOTATED changed: false basis: Mitochondrial inner-membrane carrier. Mainly resident in an intracellular compartment that MISEV2018 (PMID:30637094) lists as not enriched in small EVs of plasma-membrane/endosomal origin (category 4), and its own review keeps no experimental cell-surface or plasma-membrane pool; an exosome annotation from a bulk EV proteome is not supported. - gene: human/SLC3A1 row_index: 22 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: UNDECIDED decision: KEEP_AS_NON_CORE changed: true basis: Apical plasma-membrane transporter subunit. Transmembrane/lipid-anchored protein of the plasma membrane or endosomal system (UniProt); such proteins are expected EV contents (MISEV2018 (PMID:30637094) category 1). True, but not its core location. - gene: human/SMS row_index: 8 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic spermine synthase. Cytosolic protein (GO cytosol or cytoplasm annotation); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/STOM row_index: 38 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: ACCEPT decision: KEEP_AS_NON_CORE changed: true basis: Peripheral plasma-membrane raft protein (stomatin), released in microvesicles; expected EV content but not its core location, so the earlier ACCEPT is downgraded. Applies to the plasma microvesicle row too. Transmembrane/lipid-anchored protein of the plasma membrane or endosomal system (UniProt); such proteins are expected EV contents (MISEV2018 (PMID:30637094) category 1). True, but not its core location. - gene: human/STOM row_index: 39 term: GO:0031982 vesicle reference: PMID:19190083 previous_action: ACCEPT decision: KEEP_AS_NON_CORE changed: true basis: Peripheral plasma-membrane raft protein (stomatin), released in microvesicles; expected EV content but not its core location, so the earlier ACCEPT is downgraded. Applies to the plasma microvesicle row too. Transmembrane/lipid-anchored protein of the plasma membrane or endosomal system (UniProt); such proteins are expected EV contents (MISEV2018 (PMID:30637094) category 1). True, but not its core location. - gene: human/STOM row_index: 42 term: GO:0072562 blood microparticle reference: PMID:22516433 previous_action: ACCEPT decision: KEEP_AS_NON_CORE changed: true basis: Peripheral plasma-membrane raft protein (stomatin), released in microvesicles; expected EV content but not its core location, so the earlier ACCEPT is downgraded. Applies to the plasma microvesicle row too. Transmembrane/lipid-anchored protein of the plasma membrane or endosomal system (UniProt); such proteins are expected EV contents (MISEV2018 (PMID:30637094) category 1). True, but not its core location. - gene: human/STOM row_index: 43 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: ACCEPT decision: KEEP_AS_NON_CORE changed: true basis: Peripheral plasma-membrane raft protein (stomatin), released in microvesicles; expected EV content but not its core location, so the earlier ACCEPT is downgraded. Applies to the plasma microvesicle row too. Transmembrane/lipid-anchored protein of the plasma membrane or endosomal system (UniProt); such proteins are expected EV contents (MISEV2018 (PMID:30637094) category 1). True, but not its core location. - gene: human/STOM row_index: 44 term: GO:0070062 extracellular exosome reference: PMID:21362503 previous_action: ACCEPT decision: KEEP_AS_NON_CORE changed: true basis: Peripheral plasma-membrane raft protein (stomatin), released in microvesicles; expected EV content but not its core location, so the earlier ACCEPT is downgraded. Applies to the plasma microvesicle row too. Transmembrane/lipid-anchored protein of the plasma membrane or endosomal system (UniProt); such proteins are expected EV contents (MISEV2018 (PMID:30637094) category 1). True, but not its core location. - gene: human/SYNE2 row_index: 50 term: GO:0070062 extracellular exosome reference: PMID:18570454 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Nuclear-envelope (outer nuclear membrane) protein; lamellipodium and filopodium membrane pool by IDA (PMID:15671068). The earlier reason noted that a peptide-level hit on this giant protein does not show which isoform was detected; SYNE2 has many short isoforms, and the surface pool belongs to the protein, so the point does not change the call. Mainly resident in an intracellular compartment that MISEV2018 (PMID:30637094) lists as not enriched in small EVs (category 4), but with an experimentally documented cell-surface or plasma-membrane pool that its own review keeps; small EVs budding from the plasma membrane can carry that pool, so the detection is plausible. True, but not its core location. - gene: human/TAX1BP1 row_index: 44 term: GO:0070062 extracellular exosome reference: PMID:18570454 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic autophagy adaptor. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/THBS1 row_index: 84 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: ACCEPT decision: KEEP_AS_NON_CORE changed: true basis: Secreted thrombospondin-1 with experimental heparin and phosphatidylserine binding (IDA); the earlier ACCEPT rested only on its being secreted. MISEV2018 (PMID:30637094) category 5 allows secreted proteins onto EVs through promiscuous (proteoglycan, lipid) receptors on the EV surface, so the detection is plausible. True, but not its core location. - gene: human/THBS1 row_index: 89 term: GO:0070062 extracellular exosome reference: PMID:19199708 previous_action: ACCEPT decision: KEEP_AS_NON_CORE changed: true basis: Secreted thrombospondin-1 with experimental heparin and phosphatidylserine binding (IDA); the earlier ACCEPT rested only on its being secreted. MISEV2018 (PMID:30637094) category 5 allows secreted proteins onto EVs through promiscuous (proteoglycan, lipid) receptors on the EV surface, so the detection is plausible. True, but not its core location. - gene: human/THBS1 row_index: 90 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: ACCEPT decision: KEEP_AS_NON_CORE changed: true basis: Secreted thrombospondin-1 with experimental heparin and phosphatidylserine binding (IDA); the earlier ACCEPT rested only on its being secreted. MISEV2018 (PMID:30637094) category 5 allows secreted proteins onto EVs through promiscuous (proteoglycan, lipid) receptors on the EV surface, so the detection is plausible. True, but not its core location. - gene: human/THBS1 row_index: 108 term: GO:0070062 extracellular exosome reference: PMID:21362503 previous_action: ACCEPT decision: KEEP_AS_NON_CORE changed: true basis: Secreted thrombospondin-1 with experimental heparin and phosphatidylserine binding (IDA); the earlier ACCEPT rested only on its being secreted. MISEV2018 (PMID:30637094) category 5 allows secreted proteins onto EVs through promiscuous (proteoglycan, lipid) receptors on the EV surface, so the detection is plausible. True, but not its core location. - gene: human/THBS4 row_index: 19 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: ACCEPT decision: KEEP_AS_NON_CORE changed: true basis: Secreted thrombospondin-4 with experimental heparin binding (IDA); the earlier ACCEPT rested only on its being secreted. MISEV2018 (PMID:30637094) category 5 allows secreted proteins onto EVs through promiscuous (proteoglycan, lipid) receptors on the EV surface, so the detection is plausible. True, but not its core location. - gene: human/TKFC row_index: 18 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic kinase. Cytosolic protein (GO cytosol or cytoplasm annotation); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/TKFC row_index: 20 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic kinase. Cytosolic protein (GO cytosol or cytoplasm annotation); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/TKT row_index: 19 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic transketolase. Cytosolic protein (GO cytosol or cytoplasm annotation); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/TKT row_index: 20 term: GO:0031982 vesicle reference: PMID:19190083 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic transketolase. Cytosolic protein (GO cytosol or cytoplasm annotation); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/TKT row_index: 21 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic transketolase. Cytosolic protein (GO cytosol or cytoplasm annotation); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/TKT row_index: 22 term: GO:0070062 extracellular exosome reference: PMID:20458337 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic transketolase. Cytosolic protein (GO cytosol or cytoplasm annotation); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/TMEM63A row_index: 32 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Lysosome/endosome membrane channel. Transmembrane/lipid-anchored protein of the plasma membrane or endosomal system (UniProt); such proteins are expected EV contents (MISEV2018 (PMID:30637094) category 1). True, but not its core location. - gene: human/TOLLIP row_index: 55 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic/endosomal adaptor. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/TOLLIP row_index: 59 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic/endosomal adaptor. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/UFC1 row_index: 32 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytosolic E2 enzyme. Cytosolic protein (GO cytosol or cytoplasm annotation); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/UGGT1 row_index: 19 term: GO:0070062 extracellular exosome reference: PMID:19199708 previous_action: MARK_AS_OVER_ANNOTATED decision: MARK_AS_OVER_ANNOTATED changed: false basis: ER-lumenal glucosyltransferase with an ER-retrieval signal. Mainly resident in an intracellular compartment that MISEV2018 (PMID:30637094) lists as not enriched in small EVs of plasma-membrane/endosomal origin (category 4), and its own review keeps no experimental cell-surface or plasma-membrane pool; an exosome annotation from a bulk EV proteome is not supported. - gene: human/UGP2 row_index: 31 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic UDP-glucose pyrophosphorylase. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/UGP2 row_index: 33 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic UDP-glucose pyrophosphorylase. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/UGP2 row_index: 34 term: GO:0070062 extracellular exosome reference: PMID:20458337 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: Cytoplasmic UDP-glucose pyrophosphorylase. Cytosolic protein (UniProt); cytosolic proteins, including abundant enzymes and cytoskeletal proteins, are expected and often promiscuous EV contents (MISEV2018 (PMID:30637094) category 2b). True, but not its core location. - gene: human/VPS4A row_index: 124 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: ESCRT AAA ATPase acting on the cytosolic face of endosomes; the exosome is a destination, not where it acts. Cytosolic protein with lipid or membrane-protein binding ability; MISEV2018 (PMID:30637094) category 2a lists such proteins (ESCRT components, ALIX, annexins, HSPA8 and others) as expected EV contents. True, but not its core location. - gene: human/VPS4A row_index: 129 term: GO:0070062 extracellular exosome reference: PMID:19199708 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: ESCRT AAA ATPase acting on the cytosolic face of endosomes; the exosome is a destination, not where it acts. Cytosolic protein with lipid or membrane-protein binding ability; MISEV2018 (PMID:30637094) category 2a lists such proteins (ESCRT components, ALIX, annexins, HSPA8 and others) as expected EV contents. True, but not its core location. - gene: human/VPS4B row_index: 78 term: GO:0070062 extracellular exosome reference: PMID:23533145 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: ESCRT AAA ATPase acting on the cytosolic face of endosomes; the exosome is a destination, not where it acts. Cytosolic protein with lipid or membrane-protein binding ability; MISEV2018 (PMID:30637094) category 2a lists such proteins (ESCRT components, ALIX, annexins, HSPA8 and others) as expected EV contents. True, but not its core location. - gene: human/VPS4B row_index: 80 term: GO:0070062 extracellular exosome reference: PMID:19056867 previous_action: MARK_AS_OVER_ANNOTATED decision: KEEP_AS_NON_CORE changed: true basis: ESCRT AAA ATPase acting on the cytosolic face of endosomes; the exosome is a destination, not where it acts. Cytosolic protein with lipid or membrane-protein binding ability; MISEV2018 (PMID:30637094) category 2a lists such proteins (ESCRT components, ALIX, annexins, HSPA8 and others) as expected EV contents. True, but not its core location.