entryType: UniProtKB unreviewed (TrEMBL)

proteinExistence: 5: Uncertain

source: Google

value: Larval cuticle protein 4

value: Component of the rigid cuticle of the spider

commentType: FUNCTION

citationType: unpublished observations

database: GO

key: GoTerm

value: F:structural constituent of cuticle

key: GoEvidenceType

value: keyword

value: hydrated

entryType: UniProtKB reviewed (Swiss-Prot)

scientificName: Drosophila melanogaster

commonName: Fruit fly

lineage: Eukaryota Metazoa Ecdysozoa Arthropoda Altocrustacea Allotriocarida Hexapoda Insecta Pterygota Neoptera Eumetabola Endopterygota Aparaglossata Panorpida Diptera Brachycera Muscomorpha Ephydroidea Drosophilidae Drosophila Sophophora

proteinExistence: 1: Evidence at protein level

value: Larval cuticle protein 3

value: Larval cuticle protein III

flag: Precursor

value: Component of the larval cuticle

type: Signal

modifier: EXACT

type: Chain

description: Larval cuticle protein 3

type: Domain

description: Chitin-binding type R&R

source: PROSITE-ProRule

Cuticle

category: Cellular component

Direct protein sequencing

category: Technical term

Signal

category: Domain

citationType: journal article

authors:
Snyder M.
Hunkapiller M.
Yuen D.
Silvert D.
Fristrom J.
Davidson N.

database: PubMed

database: DOI

title: Cuticle protein genes of Drosophila: structure, organization and evolution of four clustered genes.

journal: Cell

referencePositions:
NUCLEOTIDE SEQUENCE [GENOMIC DNA]
PARTIAL PROTEIN SEQUENCE

value: Canton-S

type: STRAIN

value: Oregon-R

value: Larva

type: TISSUE

authors:
Adams M.D.
Celniker S.E.
Holt R.A.
Evans C.A.
Gocayne J.D.
Amanatides P.G.
Scherer S.E.
Li P.W.
Hoskins R.A.
Galle R.F.
George R.A.
Lewis S.E.
Richards S.
Ashburner M.
Henderson S.N.
Sutton G.G.
Wortman J.R.
Yandell M.D.
Zhang Q.
Chen L.X.
Brandon R.C.
Rogers Y.-H.C.
Blazej R.G.
Champe M.
Pfeiffer B.D.
Wan K.H.
Doyle C.
Baxter E.G.
Helt G.
Nelson C.R.
Miklos G.L.G.
Abril J.F.
Agbayani A.
An H.-J.
Andrews-Pfannkoch C.
Baldwin D.
Ballew R.M.
Basu A.
Baxendale J.
Bayraktaroglu L.
Beasley E.M.
Beeson K.Y.
Benos P.V.
Berman B.P.
Bhandari D.
Bolshakov S.
Borkova D.
Botchan M.R.
Bouck J.
Brokstein P.
Brottier P.
Burtis K.C.
Busam D.A.
Butler H.
Cadieu E.
Center A.
Chandra I.
Cherry J.M.
Cawley S.
Dahlke C.
Davenport L.B.
Davies P.
de Pablos B.
Delcher A.
Deng Z.
Mays A.D.
Dew I.
Dietz S.M.
Dodson K.
Doup L.E.
Downes M.
Dugan-Rocha S.
Dunkov B.C.
Dunn P.
Durbin K.J.
Evangelista C.C.
Ferraz C.
Ferriera S.
Fleischmann W.
Fosler C.
Gabrielian A.E.
Garg N.S.
Gelbart W.M.
Glasser K.
Glodek A.
Gong F.
Gorrell J.H.
Gu Z.
Guan P.
Harris M.
Harris N.L.
Harvey D.A.
Heiman T.J.
Hernandez J.R.
Houck J.
Hostin D.
Houston K.A.
Howland T.J.
Wei M.-H.
Ibegwam C.
Jalali M.
Kalush F.
Karpen G.H.
Ke Z.
Kennison J.A.
Ketchum K.A.
Kimmel B.E.
Kodira C.D.
Kraft C.L.
Kravitz S.
Kulp D.
Lai Z.
Lasko P.
Lei Y.
Levitsky A.A.
Li J.H.
Li Z.
Liang Y.
Lin X.
Liu X.
Mattei B.
McIntosh T.C.
McLeod M.P.
McPherson D.
Merkulov G.
Milshina N.V.
Mobarry C.
Morris J.
Moshrefi A.
Mount S.M.
Moy M.
Murphy B.
Murphy L.
Muzny D.M.
Nelson D.L.
Nelson D.R.
Nelson K.A.
Nixon K.
Nusskern D.R.
Pacleb J.M.
Palazzolo M.
Pittman G.S.
Pan S.
Pollard J.
Puri V.
Reese M.G.
Reinert K.
Remington K.
Saunders R.D.C.
Scheeler F.
Shen H.
Shue B.C.
Siden-Kiamos I.
Simpson M.
Skupski M.P.
Smith T.J.
Spier E.
Spradling A.C.
Stapleton M.
Strong R.
Sun E.
Svirskas R.
Tector C.
Turner R.
Venter E.
Wang A.H.
Wang X.
Wang Z.-Y.
Wassarman D.A.
Weinstock G.M.
Weissenbach J.
Williams S.M.
Woodage T.
Worley K.C.
Wu D.
Yang S.
Yao Q.A.
Ye J.
Yeh R.-F.
Zaveri J.S.
Zhan M.
Zhang G.
Zhao Q.
Zheng L.
Zheng X.H.
Zhong F.N.
Zhong W.
Zhou X.
Zhu S.C.
Zhu X.
Smith H.O.
Gibbs R.A.
Myers E.W.
Rubin G.M.
Venter J.C.

title: The genome sequence of Drosophila melanogaster.

journal: Science

referencePositions: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]

value: Berkeley

authors:
Misra S.
Crosby M.A.
Mungall C.J.
Matthews B.B.
Campbell K.S.
Hradecky P.
Huang Y.
Kaminker J.S.
Millburn G.H.
Prochnik S.E.
Smith C.D.
Tupy J.L.
Whitfield E.J.
Bayraktaroglu L.
Berman B.P.
Bettencourt B.R.
Celniker S.E.
de Grey A.D.N.J.
Drysdale R.A.
Harris N.L.
Richter J.
Russo S.
Schroeder A.J.
Shu S.Q.
Stapleton M.
Yamada C.
Ashburner M.
Gelbart W.M.
Rubin G.M.
Lewis S.E.

title: Annotation of the Drosophila melanogaster euchromatic genome: a systematic review.

journal: Genome Biol.

citationType: submission

authors:
Stapleton M.
Carlson J.W.
Chavez C.
Frise E.
George R.A.
Pacleb J.M.
Park S.
Wan K.H.
Yu C.
Rubin G.M.
Celniker S.E.

submissionDatabase: EMBL/GenBank/DDBJ databases

value: Pupae

database: EMBL

key: ProteinId

key: Status

key: MoleculeType

value: mRNA

database: PIR

key: EntryName

database: RefSeq

key: NucleotideSequenceId

database: AlphaFoldDB

key: Description

database: SMR

database: BioGRID

key: Interactions

database: DIP

database: FunCoup

key: Number of interactors

database: IntAct

database: STRING

value: Expressed in larva and 5 other cell types or tissues

value: F:structural constituent of chitin-based larval cuticle

value: P:larval chitin-based cuticle development

value: CUTICULAR PROTEIN 65AU, ISOFORM A-RELATED


value: ETS

value: ETS domain-containing protein

value: substring

value: Transcription factor

value: GO

proteinExistence: 2: Evidence at transcript level

value: DNA-binding protein D-ETS-4

source: PubMed

value: May have a role in germline development

commentType: SUBCELLULAR LOCATION

value: Nucleus

value: Transient high expression in pole cells during embryonic stages 8-11

commentType: TISSUE SPECIFICITY

value: Expressed throughout development with lower levels during larval development

commentType: DEVELOPMENTAL STAGE

value: Belongs to the ETS family

commentType: SIMILARITY

description: DNA-binding protein D-ETS-4

description: PNT

type: DNA binding

description: ETS

type: Region

description: Disordered

source: SAM

description: Disordered

description: Disordered

type: Compositional bias

description: Polar residues

description: Low complexity

description: Polar residues

description: Polar residues

DNA-binding

category: Molecular function

authors:
Chen T.
Bunting M.
Karim F.D.
Thummel C.S.

title: Isolation and characterization of five Drosophila genes that encode an ets-related DNA binding domain.

journal: Dev. Biol.

referencePositions: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 405-518

value: Expressed in epithelial cell in haltere and 136 other cell types or tissues

value: F:DNA-binding transcription factor activity, RNA polymerase II-specific

value: P:regulation of transcription by RNA polymerase II

value: SAM pointed domain-containing Ets transcription factor

value: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

value: SAM POINTED DOMAIN-CONTAINING ETS TRANSCRIPTION FACTOR


value: Fork-head

value: Fork-head domain-containing protein

value: F:DNA-binding transcription factor activity

value: hydrated

value: F:sequence-specific DNA binding

value: Fork head domain-containing protein FD5

value: Involved in development during embryogenesis

value: Expressed in early embryogenesis in 14 symmetrical pairs of segmentally arranged neuroblasts and in developing peripheral nervous system. Also, later in embryogenesis, in a cluster of cells in head region

value: Expressed in embryos (maximal level between 5-12 hours) and at a low level in larvae

commentType: SEQUENCE CAUTION

sequenceCautionType: Erroneous initiation

description: Fork head domain-containing protein FD5

description: Fork-head

type: Sequence conflict

description: in Ref. 4; AAF02178

originalSequence: LL

Transcription

category: Biological process

value: Berkeley

authors:
Stapleton M.
Carlson J.W.
Frise E.
Kapadia B.
Park S.
Wan K.H.
Yu C.
Celniker S.E.

value: Embryo

authors:
Haecker U.
Grossniklaus U.
Gehring W.J.
Jaeckle H.

title: Developmentally regulated Drosophila gene family encoding the fork head domain.

journal: Proc. Natl. Acad. Sci. U.S.A.

referencePositions: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-125

value: mRNA

value: Expressed in neuron of aristal sensillum (Drosophila) in antenna and 13 other cell types or tissues

value: F:RNA polymerase II cis-regulatory region sequence-specific DNA binding

value: FORK HEAD DOMAIN-CONTAINING PROTEIN FD4-RELATED


value: Nuclear hormone receptor FTZ-F1 beta

value: Nuclear hormone receptor that can act as a repressor or activator of transcription. High affinity receptor for thyroid hormones, including triiodothyronine and thyroxine

value: GO

value: Nuclear hormone receptor FTZ-F1

value: FTZ-F1 alpha

value: Nuclear receptor subfamily 5 group A member 3

value: Acts as a cofactor to fushi tarazu (ftz) (PubMed:9020363, PubMed:9020364). Facilitates the binding of ftz to DNA (PubMed:9020363, PubMed:9020364). Binds the sequence element 5'-YCYYGGYCR-3' in the zebra element of ftz (PubMed:9020363, PubMed:9020364). Probably functions as a nuclear receptor for a yet unknown ligand (PubMed:23737522, PubMed:9020363, PubMed:9020364). In contrast to vertebrate homologs, not activated by phospholipids (PubMed:23737522)

value: Monomer; forms a complex with ftz

commentType: SUBUNIT

commentType: INTERACTION

geneName: ftz

value: Nucleus

commentType: ALTERNATIVE PRODUCTS

value: B

isoformSequenceStatus: Displayed

value: A

isoformSequenceStatus: Described

value: Expression in the parasegmental primordia of the embryonic blastoderm

value: First appears in blastoderm embryos. It is absent in subsequent embryo stages, and then reappears in late embryogenesis to be found in larvae, pupae and adults

value: Ftz-like pair-rule cuticular defects

commentType: DISRUPTION PHENOTYPE

value: Belongs to the nuclear hormone receptor family. NR5 subfamily

sequenceCautionType: Frameshift

description: Nuclear hormone receptor FTZ-F1

description: NR LBD

description: Nuclear receptor

type: Zinc finger

description: NR C4-type

description: NR C4-type

description: Disordered

description: Disordered

description: Disordered

description: Disordered

description: Basic residues

description: Low complexity

description: Gly residues

description: Low complexity

description: Low complexity

description: Acidic residues

description: Gly residues

description: Low complexity

description: Gly residues

description: Gly residues

description: Low complexity

description: Gly residues

description: in isoform A

description: in isoform A

description: 100-fold less binding.

description: 100-fold less binding.

description: 100-fold less binding.

description: 10-fold less binding.

description: 10-fold less binding.

description: No binding.

description: 10-fold less reduced binding.

description: No effect on binding.

description: in Ref. 1; AA sequence

description: in Ref. 1; AAA28542

description: in Ref. 1; AAA28542 and 2; AAA28915

description: in Ref. 1; AAA28542 and 2; AAA28915

description: in Ref. 1; AAA28542 and 2; AAA28915

description: in Ref. 2; AAA28915

title: FTZ-F1, a steroid hormone receptor-like protein implicated in the activation of fushi tarazu.

referencePositions:
NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B)
PARTIAL PROTEIN SEQUENCE

authors:
Lavorgna G.
Karim F.D.
Thummel C.S.
Wu C.

title: Potential role for a TZ-F1 steroid receptor superfamily member in the control of Drosophila metamorphosis.

referencePositions:
GENOME REANNOTATION
ALTERNATIVE SPLICING

authors:
Carlson J.W.
Booth B.
Frise E.
Park S.
Wan K.H.
Yu C.
Celniker S.E.

authors:
Stapleton M.
Carlson J.W.
Brokstein P.
Yu C.
Champe M.
George R.A.
Guarin H.
Kronmiller B.
Pacleb J.M.
Park S.
Wan K.H.
Rubin G.M.
Celniker S.E.

title: A Drosophila full-length cDNA resource.

referencePositions: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 601-1027

title: A novel DNA-binding motif abuts the zinc finger domain of insect nuclear hormone receptor FTZ-F1 and mouse embryonal long terminal repeat-binding protein.

title: The Drosophila nuclear receptors FTZ-F1 alpha and FTZ-F1 beta compete as monomers for binding to a site in the fushi tarazu gene.

authors:
Guichet A.
Copeland J.W.R.
Erdelyi M.
Hlousek D.
Zavorszky P.
Ho J.
Brown S.
Percival-Smith A.
Krause H.M.
Ephrussi A.

title: The nuclear receptor homologue Ftz-F1 and the homeodomain protein Ftz are mutually dependent cofactors.

referencePositions:
FUNCTION
SUBUNIT
TISSUE SPECIFICITY
DEVELOPMENTAL STAGE

authors:
Yu Y.
Li W.
Su K.
Yussa M.
Han W.
Perrimon N.
Pick L.

title: The nuclear hormone receptor Ftz-F1 is a cofactor for the Drosophila homeodomain protein Ftz.

authors:
Musille P.M.
Pathak M.
Lauer J.L.
Griffin P.R.
Ortlund E.A.

title: Divergent sequence tunes ligand sensitivity in phospholipid-regulated hormone receptors.

value: Expressed in adult Malpighian tubule stellate cell of main segment in Malpighian tubule and 285 other cell types or tissues

value: C:RNA polymerase II transcription regulator complex

value: F:DNA-binding transcription activator activity, RNA polymerase II-specific

value: F:RNA polymerase II-specific DNA-binding transcription factor binding

value: F:transcription cis-regulatory region binding

value: P:imaginal disc-derived leg morphogenesis

value: P:juvenile hormone mediated signaling pathway

value: P:positive regulation of transcription by RNA polymerase II

value: P:regulation of development, heterochronic

value: P:regulation of DNA-templated transcription

value: P:regulation of transcription by RNA polymerase II

value: Erythroid Transcription Factor GATA-1, subunit A

value: Glucocorticoid receptor-like (DNA-binding domain)


value: Helicase C-terminal

value: domain

value: Helicase C-terminal domain-containing protein

value: C:intracellular organelle

value: keyword

value: hydrated

value: Transcription termination factor 2

value: Protein lodestar

value: RNA polymerase II termination factor

value: Transcription release factor 2

value: lds

value: DsDNA-dependent ATPase which acts as a transcription termination factor by coupling ATP hydrolysis with removal of RNA polymerase II from the DNA template

value: Nucleus

value: Belongs to the SNF2/RAD54 helicase family

description: Transcription termination factor 2

description: Helicase ATP-binding

description: Helicase C-terminal

description: Disordered

description: Disordered

description: Disordered

type: Motif

description: DEAH box

description: Low complexity

description: Acidic residues

description: Polar residues

description: Basic residues

type: Binding site

database: ChEBI

type: Modified residue

description: Phosphoserine

description: Phosphoserine

description: Phosphoserine

description: Phosphoserine

description: Phosphothreonine

description: in Ref. 1; CAA44496

originalSequence: R

title: Chromosome tangling and breakage at anaphase result from mutations in lodestar, a Drosophila gene encoding a putative nucleoside triphosphate-binding protein.

title: A human RNA polymerase II transcription termination factor is a SWI2/SNF2 family member.

authors:
Zhai B.
Villen J.
Beausoleil S.A.
Mintseris J.
Gygi S.P.

title: Phosphoproteome analysis of Drosophila melanogaster embryos.

referencePositions: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-108; SER-110; SER-214; SER-215 AND THR-216

value: Expressed in secondary oocyte and 57 other cell types or tissues

value: F:ATP-dependent chromatin remodeler activity

value: P-loop containing nucleotide triphosphate hydrolases

value: TRANSCRIPTION TERMINATION FACTOR 2-RELATED

value: P-loop containing nucleoside triphosphate hydrolases


value: Uncharacterized protein

value: Secreted

value: Lysozyme E

value: 1,4-beta-N-acetylmuramidase E

value: LysE

value: Unlikely to play an active role in the humoral immune defense. May have a function in the digestion of bacteria in the food

commentType: CATALYTIC ACTIVITY

name: Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.

value: Found in the midgut

value: Maximal expression is found during the third larval instar, it drops to become undetectable in the late pupal stage. The expression in adults is similar to that of first and second larval instars

value: Belongs to the glycosyl hydrolase 22 family

description: Lysozyme E

description: C-type lysozyme

type: Active site

type: Disulfide bond

description: in Ref. 1; CAA80229

originalSequence: M

description: in Ref. 1; CAA80229

originalSequence: N

description: in Ref. 1; CAA80229

originalSequence: G

Disulfide bond

category: PTM

authors:
Daffre S.
Kylsten P.
Samakovlis C.
Hultmark D.

title: The lysozyme locus in Drosophila melanogaster: an expanded gene family adapted for expression in the digestive tract.

journal: Mol. Gen. Genet.

value: Canton-S

value: Berkeley

authors:
Stapleton M.
Brokstein P.
Hong L.
Agbayani A.
Carlson J.W.
Booth B.
Champe M.
Chavez C.
Dorsett V.
Dresnek D.
Farfan D.
Frise E.
George R.A.
Gonzalez M.
Guarin H.
Kronmiller B.
Li P.W.
Liao G.
Miranda A.
Mungall C.J.
Nunoo J.
Pacleb J.
Paragas V.
Park S.
Patel S.
Phouanenavong S.
Sandler J.
Wan K.H.
Yu C.
Lewis S.E.
Rubin G.M.
Celniker S.E.

value: Larva

value: Pupae

value: Expressed in spermatid in male reproductive gland and 17 other cell types or tissues

value: P:defense response to Gram-negative bacterium

value: P:negative regulation of innate immune response


value: F:structural constituent of cuticle

value: GO

value: hydrated

value: Protein lethal(3)malignant blood neoplasm 1

value: Required for differentiation of the phagocytic blood-cell type, the plasmatocyte

value: Mainly around the nucleus

value: Cytoplasm

value: Cytoplasm, perinuclear region

value: Blood cells and other tissues

sequenceCautionType: Miscellaneous discrepancy

note: Intron retention.

description: Chitin-binding type R&R 1

type: Repeat

description: Chitin-binding type R&R 2

description: 2 X 21 AA repeats

description: Disordered

description: 7 X approximate tandem repeats, Gly/Ser-rich

description: Gly residues

description: in Ref. 1; CAA87654

originalSequence: E

description: in Ref. 1; CAA87654

originalSequence: H

description: in Ref. 1; CAA87654

originalSequence: A

description: in Ref. 1; CAA87654

originalSequence: P

description: in Ref. 1; CAA87654

description: in Ref. 1; CAA87654

description: in Ref. 1; CAA87654

description: in Ref. 1; CAA87654

description: in Ref. 1; CAA87654

description: in Ref. 1; CAA87654

description: in Ref. 1; CAA87654

authors:
Konrad L.
Becker G.
Schmidt A.
Kloeckner T.
Kaufer-Stillger G.
Dreschers S.
Edstroem J.-E.
Gateff E.

title: Cloning, structure, cellular localization, and possible function of the tumor suppressor gene lethal(3)malignant blood neoplasm-1 of Drosophila melanogaster.

referencePositions:
NUCLEOTIDE SEQUENCE [GENOMIC DNA]
FUNCTION
SUBCELLULAR LOCATION
TISSUE SPECIFICITY

title: A Drosophila full-length cDNA resource.

referencePositions: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-298

value: Expressed in thoracico-abdominal ganglion (Drosophila) and 4 other cell types or tissues

value: F:structural constituent of chitin-based larval cuticle

value: PROTEIN LETHAL(3)MALIGNANT BLOOD NEOPLASM 1


value: substring

value: Cytoplasmic vesicle lumen

value: Involved in the maturation of Asn-linked oligosaccharides. Progressively trims alpha-1,2-linked mannose residues from Man(9)GlcNAc(2) to produce Man(5)GlcNAc(2)

source: FlyBase

source: UniProtKB

value: alpha-Man-Ia

value: Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man(9)GlcNAc(2) to produce Man(5)GlcNAc(2)

name: N(4)-(alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 9A1,2,3B1,2,3) + 4 H2O = N(4)-(alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2) + 4 beta-D-mannose

database: Rhea

name: N(4)-(alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 8A1,2,3B1,3) + 3 H2O = N(4)-(alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2) + 3 beta-D-mannose

commentType: COFACTOR

value: Protein modification; protein glycosylation

commentType: PATHWAY

value: Golgi apparatus membrane

value: Single-pass type II membrane protein

value: H

value: A

value: J

value: L

value: M

value: N

value: O

value: K

value: B

value: P

value: Complex spatial distribution during embryogenesis, including expression in lobula plate giant neurons. Also expressed in adult wing and eyes

value: Expressed both maternally and zygotically during embryonic stages

source: RuleBase

value: Belongs to the glycosyl hydrolase 47 family

description: Mannosyl-oligosaccharide alpha-1,2-mannosidase IA

type: Topological domain

description: Cytoplasmic

type: Transmembrane

description: Helical; Signal-anchor for type II membrane protein

description: Lumenal

description: Disordered

description: Low complexity

description: Proton donor

description: N-linked (GlcNAc...) asparagine

description: in isoform K

description: in Ref. 1; CAA57962

description: in Ref. 1; CAA57962

authors:
Kerscher S.
Albert S.
Wucherpfennig D.
Heisenberg M.
Schneuwly S.

title: Molecular and genetic analysis of the Drosophila mas-1 (mannosidase-1) gene which encodes a glycoprotein processing alpha 1,2-mannosidase.

referencePositions: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS H AND K)

authors:
Stapleton M.
Brokstein P.
Hong L.
Agbayani A.
Carlson J.W.
Champe M.
Chavez C.
Dorsett V.
Dresnek D.
Farfan D.
Frise E.
George R.A.
Gonzalez M.
Guarin H.
Kronmiller B.
Li P.W.
Liao G.
Miranda A.
Mungall C.J.
Nunoo J.
Pacleb J.M.
Paragas V.
Park S.
Patel S.
Phouanenavong S.
Wan K.H.
Yu C.
Lewis S.E.
Rubin G.M.
Celniker S.E.

value: Progressive trimming of alpha-1,2-linked mannose residues from Man9/8/7GlcNAc2 to produce Man5GlcNAc2

value: Expressed in distal medullary amacrine neuron Dm1-5 (Drosophila) in insect head and 274 other cell types or tissues

value: P:Golgi apparatus N-glycan mannose trimming


value: Protein kinase

value: domain

value: Protein kinase domain-containing protein

value: GO

value: hydrated

value: Cytoplasm

value: Dual specificity mitogen-activated protein kinase kinase dSOR1

value: Downstream of RAF

value: MAPKK

value: Required downstream of Raf in the sevenless (sev), torso (tor), and Drosophila EGF receptor homolog (DER) signal transduction pathways. Involved in both positive regulation (at the posterior terminus) and negative regulation (at the anterior domain) of tll, as in other terminal class gene products, maybe via the ERK-A kinase

name: L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)

name: L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)

name: L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)

value: Interacts with Raf and ksr; Dsor1 binding to ksr probably promotes ksr and Raf dimerization and ksr-mediated Raf transactivation

geneName: Raf

value: Expressed both maternally and zygotically

value: Phosphorylation on Ser/Thr by MAP kinase kinase kinases regulates positively the kinase activity

commentType: PTM

value: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily

sequenceCautionType: Erroneous gene model prediction

type: Chain

description: Protein kinase

description: Disordered

description: Proton acceptor

description: Phosphoserine; by RAF

description: Phosphoserine; by RAF

type: Natural variant

description: in strain: Reids2

originalSequence: S

type: Helix

source: PDB

type: Beta strand

authors:
Tsuda L.
Inoue Y.H.
Yoo M.-A.
Mizuno M.
Hata M.
Lim Y.-M.
Adachi-Yamada T.
Ryo H.
Masamune Y.
Nishida Y.

title: A protein kinase similar to MAP kinase activator acts downstream of the raf kinase in Drosophila.

referencePositions:
NUCLEOTIDE SEQUENCE [GENOMIC DNA]
FUNCTION
DEVELOPMENTAL STAGE

title: Contrasting selection pressures on components of the Ras-mediated signal transduction pathway in Drosophila.

authors:
Adams M.D.
Celniker S.E.
Holt R.A.
Evans C.A.
Gocayne J.D.
Amanatides P.G.
Scherer S.E.
Li P.W.
Hoskins R.A.
Galle R.F.
George R.A.
Lewis S.E.
Richards S.
Ashburner M.
Henderson S.N.
Sutton G.G.
Wortman J.R.
Yandell M.D.
Zhang Q.
Chen L.X.
Brandon R.C.
Rogers Y.-H.C.
Blazej R.G.
Champe M.
Pfeiffer B.D.
Wan K.H.
Doyle C.
Baxter E.G.
Helt G.
Nelson C.R.
Miklos G.L.G.
Abril J.F.
Agbayani A.
An H.-J.
Andrews-Pfannkoch C.
Baldwin D.
Ballew R.M.
Basu A.
Baxendale J.
Bayraktaroglu L.
Beasley E.M.
Beeson K.Y.
Benos P.V.
Berman B.P.
Bhandari D.
Bolshakov S.
Borkova D.
Botchan M.R.
Bouck J.
Brokstein P.
Brottier P.
Burtis K.C.
Busam D.A.
Butler H.
Cadieu E.
Center A.
Chandra I.
Cherry J.M.
Cawley S.
Dahlke C.
Davenport L.B.
Davies P.
de Pablos B.
Delcher A.
Deng Z.
Mays A.D.
Dew I.
Dietz S.M.
Dodson K.
Doup L.E.
Downes M.
Dugan-Rocha S.
Dunkov B.C.
Dunn P.
Durbin K.J.
Evangelista C.C.
Ferraz C.
Ferriera S.
Fleischmann W.
Fosler C.
Gabrielian A.E.
Garg N.S.
Gelbart W.M.
Glasser K.
Glodek A.
Gong F.
Gorrell J.H.
Gu Z.
Guan P.
Harris M.
Harris N.L.
Harvey D.A.
Heiman T.J.
Hernandez J.R.
Houck J.
Hostin D.
Houston K.A.
Howland T.J.
Wei M.-H.
Ibegwam C.
Jalali M.
Kalush F.
Karpen G.H.
Ke Z.
Kennison J.A.
Ketchum K.A.
Kimmel B.E.
Kodira C.D.
Kraft C.L.
Kravitz S.
Kulp D.
Lai Z.
Lasko P.
Lei Y.
Levitsky A.A.
Li J.H.
Li Z.
Liang Y.
Lin X.
Liu X.
Mattei B.
McIntosh T.C.
McLeod M.P.
McPherson D.
Merkulov G.
Milshina N.V.
Mobarry C.
Morris J.
Moshrefi A.
Mount S.M.
Moy M.
Murphy B.
Murphy L.
Muzny D.M.
Nelson D.L.
Nelson D.R.
Nelson K.A.
Nixon K.
Nusskern D.R.
Pacleb J.M.
Palazzolo M.
Pittman G.S.
Pan S.
Pollard J.
Puri V.
Reese M.G.
Reinert K.
Remington K.
Saunders R.D.C.
Scheeler F.
Shen H.
Shue B.C.
Siden-Kiamos I.
Simpson M.
Skupski M.P.
Smith T.J.
Spier E.
Spradling A.C.
Stapleton M.
Strong R.
Sun E.
Svirskas R.
Tector C.
Turner R.
Venter E.
Wang A.H.
Wang X.
Wang Z.-Y.
Wassarman D.A.
Weinstock G.M.
Weissenbach J.
Williams S.M.
Woodage T.
Worley K.C.
Wu D.
Yang S.
Yao Q.A.
Ye J.
Yeh R.-F.
Zaveri J.S.
Zhan M.
Zhang G.
Zhao Q.
Zheng L.
Zheng X.H.
Zhong F.N.
Zhong W.
Zhou X.
Zhu S.C.
Zhu X.
Smith H.O.
Gibbs R.A.
Myers E.W.
Rubin G.M.
Venter J.C.

title: The genome sequence of Drosophila melanogaster.

authors:
Misra S.
Crosby M.A.
Mungall C.J.
Matthews B.B.
Campbell K.S.
Hradecky P.
Huang Y.
Kaminker J.S.
Millburn G.H.
Prochnik S.E.
Smith C.D.
Tupy J.L.
Whitfield E.J.
Bayraktaroglu L.
Berman B.P.
Bettencourt B.R.
Celniker S.E.
de Grey A.D.N.J.
Drysdale R.A.
Harris N.L.
Richter J.
Russo S.
Schroeder A.J.
Shu S.Q.
Stapleton M.
Yamada C.
Ashburner M.
Gelbart W.M.
Rubin G.M.
Lewis S.E.

title: A Drosophila full-length cDNA resource.

authors:
Lavoie H.
Sahmi M.
Maisonneuve P.
Marullo S.A.
Thevakumaran N.
Jin T.
Kurinov I.
Sicheri F.
Therrien M.

title: MEK drives BRAF activation through allosteric control of KSR proteins.

referencePositions:
INTERACTION WITH KSR AND RAF
PHOSPHORYLATION AT SER-237 AND SER-241

value: Negative feedback regulation of MAPK pathway

value: Expressed in T neuron T5a (Drosophila) in embryonic/larval optic lobe (Drosophila) and 146 other cell types or tissues

value: P:epidermal growth factor receptor signaling pathway

value: P:fibroblast growth factor receptor signaling pathway

value: P:mitotic DNA replication checkpoint signaling

value: P:mitotic G2 DNA damage checkpoint signaling

value: P:terminal branching, open tracheal system

value: P:vascular endothelial growth factor receptor signaling pathway

value: Dual specificity mitogen-activated protein kinase kinase 1

value: DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE DSOR1-LIKE PROTEIN

value: SERINE_THREONINE-PROTEIN KINASE STE7 HOMOLOG


value: Peptidase S1

value: domain

value: Peptidase S1 domain-containing protein

value: F:serine-type endopeptidase activity

source: Proteomes

value: Inactive serine protease scarface

value: scaf

value: scarf

value: Inactive serine protease that plays a role in germ-band retraction and dorsal closure morphogenesis in embryogenesis; contributes to amnioserosa attachment and epithelial apico-basal polarity by regulating the localization of laminin LanA on the apical side of the amnioserosa epithelium (PubMed:20379222, PubMed:28628612). Contributes to epithelial morphogenesis probably by regulating the bsk/JNK pathway, as part of a negative-feedback loop, and by modulating the cross-talk between the Egfr, bsk/JNK and dpp signal transduction pathways (PubMed:20379222, PubMed:28628612). In larval development, antagonizes the morphogenetic movements controlled by the bsk/JNK signaling including male genitalia formation and thorax development (PubMed:20379222, PubMed:20530545, PubMed:25737837)

value: Secreted

value: In embryos, expressed in leading edge (LE) cells (at protein level) (PubMed:20530545). In larvae, expressed in the wing imaginal disk cells in the future hinge region, specifically in the peripodial stalk and in the peripodial membrane cells (at protein level) (PubMed:15342518, PubMed:25737837). In larvae, expressed in the A8 abdomen-derived cells in the male genital disk (at protein level) (PubMed:20530545). In third instar larvae, expressed in the anterior and posterios termini (at protein level) (PubMed:15342518). In adult fly, expressed in the wing hinge, in the socket cells of the micro- and macrochaete and proboscis (at protein level) (PubMed:15342518). In embryos, expressed in leading edge (LE) cells during germ-band retraction and dorsal closure from stage 13; expressed in some cells of the amnioserosa in particular in the posterior canthus as well as in the ventral ectoderm; expressed both in head and tail region (PubMed:20379222, PubMed:20530545, PubMed:28628612)

value: The CLIP domain consists of 37-55 residues which are 'knitted' together usually by 3 conserved disulfide bonds forming a clip-like compact structure

commentType: DOMAIN

value: Embryonic lethal due to defects in the dorsal closure and germ-band retraction; these defects include undifferentiated or defective larval cuticles presenting dorsal holes and wrinkles, defective attachment of the amnioserosa to the tail end of the germ band, defective elongation of the lateral ectoderm with compromised interface between the LE cells and the amnioserosa (PubMed:15342518, PubMed:20379222, PubMed:20530545). RNAi-mediated knockdown results in pupal lethality and scarring (PubMed:25737837). RNAi-mediated knockdown in the epidermis results in loss of wing veins and thorax mechanosensory bristles as well as male terminalia malformations including genitalia rotation defects and partial dissociation of the genital plate from the abdomen (PubMed:20530545). RNAi-mediated knockdown in the dorsal compartment of the wing disk results in loss of bristles from the medio-lateral region of the thorax and in the appearance of a mild thoracic cleft (PubMed:25737837). RNAi-mediated knockdown in the notum area of the wing disk, destined to form the dorsal medio-lateral region of the adult thorax as well as thoracic bristles, results in a thoracic cleft and loss of bristles from the medio-lateral region of the thorax (PubMed:25737837)

value: Belongs to the peptidase S1 family

value: Lacks the conserved residues within the catalytic triad, probably resulting in a loss of proteolytic activity

commentType: CAUTION

type: Signal

description: Inactive serine protease scarface

type: Domain

description: Peptidase S1

description: Disordered

description: CLIP

description: Polar residues

description: Polar residues

title: Annotation of the Drosophila melanogaster euchromatic genome: a systematic review.

title: A Drosophila full-length cDNA resource.

title: A piggyBac transposon gene trap for the analysis of gene expression and function in Drosophila.

authors:
Sorrosal G.
Perez L.
Herranz H.
Milan M.

title: Scarface, a secreted serine protease-like protein, regulates polarized localization of laminin A at the basement membrane of the Drosophila embryo.

referencePositions:
FUNCTION
SUBCELLULAR LOCATION
DEVELOPMENTAL STAGE
DISRUPTION PHENOTYPE

authors:
Rousset R.
Bono-Lauriol S.
Gettings M.
Suzanne M.
Speder P.
Noselli S.

title: The Drosophila serine protease homologue Scarface regulates JNK signalling in a negative-feedback loop during epithelial morphogenesis.

title: Regulation of a serine protease homolog by the JNK pathway during thoracic development of Drosophila melanogaster.

referencePositions:
FUNCTION
DEVELOPMENTAL STAGE
DISRUPTION PHENOTYPE

authors:
Kushnir T.
Mezuman S.
Bar-Cohen S.
Lange R.
Paroush Z.
Helman A.

title: Novel interplay between JNK and Egfr signaling in Drosophila dorsal closure.

value: Expressed in tormogen cell in proboscis and 65 other cell types or tissues

value: P:dorsal closure, elongation of leading edge cells

value: P:establishment or maintenance of epithelial cell apical/basal polarity

value: P:imaginal disc-derived male genitalia morphogenesis


value: Putative gustatory receptor 85a

value: substring

value: Gustatory receptor

value: Probable gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates.

value: Gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates

value: keyword

value: Membrane

source: EMBL

proteinExistence: 3: Inferred from homology

value: Cell membrane

value: Multi-pass membrane protein

value: Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family. Gr22e subfamily

description: Putative gustatory receptor 85a

description: Cytoplasmic

description: Helical; Name=1

description: Extracellular

description: Helical; Name=2

description: Cytoplasmic

description: Helical; Name=3

description: Extracellular

description: Helical; Name=4

description: Cytoplasmic

description: Helical; Name=5

description: Extracellular

description: Helical; Name=6

description: Cytoplasmic

description: Helical; Name=7

description: Extracellular

value: Berkeley

type: STRAIN

authors:
Dunipace L.
Meister S.
McNealy C.
Amrein H.

title: Spatially restricted expression of candidate taste receptors in the Drosophila gustatory system.

journal: Curr. Biol.

value: F:ligand-gated monoatomic ion channel activity

value: P:monoatomic ion transmembrane transport


value: Uncharacterized protein

value: Golgi apparatus membrane

value: Glycosaminoglycan xylosylkinase homolog

value: Xylose kinase homolog

value: Kylose kinase that mediates the 2-O-phosphorylation of xylose in the glycosaminoglycan-protein linkage region of proteoglycans

name: 3-O-(beta-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-xylosyl)-L-seryl-[protein] + ATP = 3-O-(beta-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-2-O-phosphoxylosyl)-L-seryl-[protein] + ADP + H(+)

value: Golgi apparatus

value: Endoplasmic reticulum

value: Belongs to the FAM20 family

description: Glycosaminoglycan xylosylkinase homolog

type: Glycosylation

description: N-linked (GlcNAc...) asparagine

authors:
Stapleton M.
Carlson J.W.
Brokstein P.
Yu C.
Champe M.
George R.A.
Guarin H.
Kronmiller B.
Pacleb J.M.
Park S.
Wan K.H.
Rubin G.M.
Celniker S.E.

title: A Drosophila full-length cDNA resource.

authors:
Ishikawa H.O.
Xu A.
Ogura E.
Manning G.
Irvine K.D.

title: The Raine syndrome protein FAM20C is a Golgi kinase that phosphorylates bio-mineralization proteins.

value: Glycosaminoglycan-protein linkage region biosynthesis

value: Expressed in posterior terminal follicle cell in ovary and 85 other cell types or tissues

value: F:phosphotransferase activity, alcohol group as acceptor

value: F:protein serine/threonine kinase activity


value: PANTHER

value: Odorant receptor

value: Odorant receptor 19a

value: Odorant receptor which mediates acceptance or avoidance behavior, depending on its substrates. The odorant receptor repertoire encodes a large collection of odor stimuli that vary widely in identity, intensity, and duration. May form a complex with Orco to form odorant-sensing units, providing sensitive and prolonged odorant signaling and calcium permeability. Involved in the preference for citrus fruits for oviposition, especially through the response to valencene, the primary ligand of Or19a. Larvae growing on citrus fruits suffer a reduced risk of parasitism since endoparasitoid wasps that parasitize larvae are strongly repelled by the smell of citrus, as well as by valencene

value: Interacts with Orco. Complexes exist early in the endomembrane system in olfactory sensory neurons (OSNs), coupling these complexes to the conserved ciliary trafficking pathway (By similarity)

value: Cell membrane

value: Multi-pass membrane protein

value: Expressed in ai2A olfactory sensory neurons in the antenna

value: The atypical heteromeric and topological design of the odorant receptors appears to be an insect-specific solution for odor recognition, making the OR/Orco complex an attractive target for the development of highly selective insect repellents to disrupt olfactory-mediated host-seeking behaviors of insect disease vectors. Odor-evoked OR currents are independent of known G protein-coupled second messenger pathways

commentType: MISCELLANEOUS

value: Belongs to the insect chemoreceptor superfamily. Heteromeric odorant receptor channel (TC 1.A.69) family. Or2a subfamily

description: Odorant receptor 19a

description: Cytoplasmic

description: Helical; Name=1

description: Extracellular

description: Helical; Name=2

description: Cytoplasmic

description: Helical; Name=3

description: Extracellular

description: Helical; Name=4

description: Cytoplasmic

description: Helical; Name=5

description: Extracellular

description: Helical; Name=6

description: Cytoplasmic

description: Helical; Name=7

description: Extracellular

authors:
Vosshall L.B.
Wong A.M.
Axel R.

title: An olfactory sensory map in the fly brain.

authors:
Hallem E.A.
Carlson J.R.

title: Coding of odors by a receptor repertoire.

authors:
Dweck H.K.
Ebrahim S.A.
Kromann S.
Bown D.
Hillbur Y.
Sachse S.
Hansson B.S.
Stensmyr M.C.

title: Olfactory preference for egg laying on citrus substrates in Drosophila.

value: Expressed in adult olfactory receptor neuron Or19 (Drosophila)

value: F:ionotropic olfactory receptor activity

value: P:detection of chemical stimulus involved in sensory perception of smell


value: Polycomb protein Su(z)12

value: Polycomb protein Su(Z)12

value: P:chromatin remodeling

value: Suppressor 12 of zeste protein

value: Polycomb group (PcG) protein. While PcG proteins are generally required to maintain the transcriptionally repressive state of homeotic genes throughout development, this protein is specifically required during the first 6 hours of embryogenesis to establish the repressed state. Component of the Esc/E(z) complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. The Esc/E(z) complex is necessary but not sufficient for the repression of homeotic target genes, suggesting that the recruitment of the distinct PRC1 complex is also required

commentType: FUNCTION

value: Component of the polycomb repressive complex 2 (PRC2, also known as the Esc/E(Z) complex), composed of Caf1-55, esc, E(z), Su(z)12, and possibly pho (PubMed:12408863, PubMed:12408864). PRC2 associates with the accessory components Jarid2 and jing to form the PRC2 Jarid2-jing variant (PRC2.2) (PubMed:22354997). PRC2 may also associate with Pcl and HDAC1/Rpd3 during early embryogenesis (PubMed:12408863, PubMed:12408864, PubMed:12697833). This complex is distinct from the PRC1 complex, which contains many other PcG proteins like Pc, Ph, Psc, Su(z)2 (Probable). The two complexes however cooperate and interact together during the first 3 hours of development to establish PcG silencing (Probable)

value: Nucleus

value: Chromosome

value: B

value: A

value: Belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family

description: Polycomb protein Su(z)12

type: Zinc finger

type: Region

description: Disordered

description: VEFS-box

description: Disordered

type: Compositional bias

description: Basic and acidic residues

description: Low complexity

description: Low complexity

description: Basic and acidic residues

description: Gly residues

description: Low complexity

description: Phosphoserine

type: Alternative sequence

description: in isoform 2

description: in isoform 2

type: Mutagenesis

description: In Su(z)12-2; induces larval lethality; when homozygous.

authors:
Birve A.
Sengupta A.K.
Beuchle D.
Larsson J.
Kennison J.A.
Rasmuson-Lestander A.
Mueller J.

title: Su(z)12, a novel Drosophila Polycomb group gene that is conserved in vertebrates and plants.

referencePositions:
NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
CHARACTERIZATION
MUTAGENESIS OF GLY-274

title: A Drosophila full-length cDNA resource.

referencePositions: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2)

authors:
Czermin B.
Melfi R.
McCabe D.
Seitz V.
Imhof A.
Pirrotta V.

title: Drosophila Enhancer of zeste/ESC complexes have a histone H3 methyltransferase activity that marks chromosomal Polycomb sites.

referencePositions: IDENTIFICATION IN AN ESC/E(Z) COMPLEX WITH CAF1-55; ESC; E(Z) AND HDAC1

referencePositions: METHYLTRANSFERASE ACTIVITY OF THE COMPLEX

authors:
Mueller J.
Hart C.M.
Francis N.J.
Vargas M.L.
Sengupta A.
Wild B.
Miller E.L.
O'Connor M.B.
Kingston R.E.
Simon J.A.

title: Histone methyltransferase activity of a Drosophila Polycomb group repressor complex.

referencePositions: IDENTIFICATION IN AN ESC/E(Z) COMPLEX WITH CAF1-55; ESC AND E(Z)

authors:
Tie F.
Prasad-Sinha J.
Birve A.
Rasmuson-Lestander A.
Harte P.J.

title: A 1-megadalton ESC/E(Z) complex from Drosophila that contains polycomblike and RPD3.

referencePositions: IDENTIFICATION IN AN ESC/E(Z) COMPLEX WITH CAF1-55; ESC; E(Z); PCL AND HDAC1

authors:
Bodenmiller B.
Mueller L.N.
Pedrioli P.G.A.
Pflieger D.
Juenger M.A.
Eng J.K.
Aebersold R.
Tao W.A.

title: An integrated chemical, mass spectrometric and computational strategy for (quantitative) phosphoproteomics: application to Drosophila melanogaster Kc167 cells.

referencePositions: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-547

authors:
Herz H.M.
Mohan M.
Garrett A.S.
Miller C.
Casto D.
Zhang Y.
Seidel C.
Haug J.S.
Florens L.
Washburn M.P.
Yamaguchi M.
Shiekhattar R.
Shilatifard A.

title: Polycomb repressive complex 2-dependent and -independent functions of Jarid2 in transcriptional regulation in Drosophila.

referencePositions:
IDENTIFICATION IN THE PRC2.2 COMPLEX
SUBCELLULAR LOCATION
IDENTIFICATION BY MASS SPECTROMETRY

value: Polycomb repressive complex 2, Pcl variant

value: Polycomb repressive complex 2, Jarid2-jing variant

value: Negative Regulation of CDH1 Gene Transcription

value: Regulation of PD-L1(CD274) transcription

value: Expressed in fat body cell in Malpighian tubule and 153 other cell types or tissues

value: P:negative regulation of DNA-templated transcription

value: P:negative regulation of response to gamma radiation

value: P:negative regulation of transcription by RNA polymerase II


value: PANTHER

value: Protein FAM50A

commentType: SUBCELLULAR LOCATION

value: GO

value: Probably involved in the regulation of pre-mRNA splicing

value: Protein FAM50 homolog

value: Belongs to the FAM50 family

description: Protein FAM50 homolog

description: Disordered

description: Disordered

description: Acidic residues

description: Basic and acidic residues

authors:
Adams M.D.
Celniker S.E.
Holt R.A.
Evans C.A.
Gocayne J.D.
Amanatides P.G.
Scherer S.E.
Li P.W.
Hoskins R.A.
Galle R.F.
George R.A.
Lewis S.E.
Richards S.
Ashburner M.
Henderson S.N.
Sutton G.G.
Wortman J.R.
Yandell M.D.
Zhang Q.
Chen L.X.
Brandon R.C.
Rogers Y.-H.C.
Blazej R.G.
Champe M.
Pfeiffer B.D.
Wan K.H.
Doyle C.
Baxter E.G.
Helt G.
Nelson C.R.
Miklos G.L.G.
Abril J.F.
Agbayani A.
An H.-J.
Andrews-Pfannkoch C.
Baldwin D.
Ballew R.M.
Basu A.
Baxendale J.
Bayraktaroglu L.
Beasley E.M.
Beeson K.Y.
Benos P.V.
Berman B.P.
Bhandari D.
Bolshakov S.
Borkova D.
Botchan M.R.
Bouck J.
Brokstein P.
Brottier P.
Burtis K.C.
Busam D.A.
Butler H.
Cadieu E.
Center A.
Chandra I.
Cherry J.M.
Cawley S.
Dahlke C.
Davenport L.B.
Davies P.
de Pablos B.
Delcher A.
Deng Z.
Mays A.D.
Dew I.
Dietz S.M.
Dodson K.
Doup L.E.
Downes M.
Dugan-Rocha S.
Dunkov B.C.
Dunn P.
Durbin K.J.
Evangelista C.C.
Ferraz C.
Ferriera S.
Fleischmann W.
Fosler C.
Gabrielian A.E.
Garg N.S.
Gelbart W.M.
Glasser K.
Glodek A.
Gong F.
Gorrell J.H.
Gu Z.
Guan P.
Harris M.
Harris N.L.
Harvey D.A.
Heiman T.J.
Hernandez J.R.
Houck J.
Hostin D.
Houston K.A.
Howland T.J.
Wei M.-H.
Ibegwam C.
Jalali M.
Kalush F.
Karpen G.H.
Ke Z.
Kennison J.A.
Ketchum K.A.
Kimmel B.E.
Kodira C.D.
Kraft C.L.
Kravitz S.
Kulp D.
Lai Z.
Lasko P.
Lei Y.
Levitsky A.A.
Li J.H.
Li Z.
Liang Y.
Lin X.
Liu X.
Mattei B.
McIntosh T.C.
McLeod M.P.
McPherson D.
Merkulov G.
Milshina N.V.
Mobarry C.
Morris J.
Moshrefi A.
Mount S.M.
Moy M.
Murphy B.
Murphy L.
Muzny D.M.
Nelson D.L.
Nelson D.R.
Nelson K.A.
Nixon K.
Nusskern D.R.
Pacleb J.M.
Palazzolo M.
Pittman G.S.
Pan S.
Pollard J.
Puri V.
Reese M.G.
Reinert K.
Remington K.
Saunders R.D.C.
Scheeler F.
Shen H.
Shue B.C.
Siden-Kiamos I.
Simpson M.
Skupski M.P.
Smith T.J.
Spier E.
Spradling A.C.
Stapleton M.
Strong R.
Sun E.
Svirskas R.
Tector C.
Turner R.
Venter E.
Wang A.H.
Wang X.
Wang Z.-Y.
Wassarman D.A.
Weinstock G.M.
Weissenbach J.
Williams S.M.
Woodage T.
Worley K.C.
Wu D.
Yang S.
Yao Q.A.
Ye J.
Yeh R.-F.
Zaveri J.S.
Zhan M.
Zhang G.
Zhao Q.
Zheng L.
Zheng X.H.
Zhong F.N.
Zhong W.
Zhou X.
Zhu S.C.
Zhu X.
Smith H.O.
Gibbs R.A.
Myers E.W.
Rubin G.M.
Venter J.C.

title: The genome sequence of Drosophila melanogaster.

referencePositions: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]

value: Berkeley

authors:
Misra S.
Crosby M.A.
Mungall C.J.
Matthews B.B.
Campbell K.S.
Hradecky P.
Huang Y.
Kaminker J.S.
Millburn G.H.
Prochnik S.E.
Smith C.D.
Tupy J.L.
Whitfield E.J.
Bayraktaroglu L.
Berman B.P.
Bettencourt B.R.
Celniker S.E.
de Grey A.D.N.J.
Drysdale R.A.
Harris N.L.
Richter J.
Russo S.
Schroeder A.J.
Shu S.Q.
Stapleton M.
Yamada C.
Ashburner M.
Gelbart W.M.
Rubin G.M.
Lewis S.E.

title: Annotation of the Drosophila melanogaster euchromatic genome: a systematic review.

title: A Drosophila full-length cDNA resource.

value: Head

type: TISSUE

value: mRNA

database: PaxDb

database: PeptideAtlas

database: DNASU

database: EnsemblMetazoa

key: GeneId

database: GeneID

database: KEGG

database: UCSC

key: OrganismName

value: d. melanogaster

database: AGR

database: FlyBase

key: GeneName

database: VEuPathDB

value: Expressed in adult middle midgut class II enteroendocrine cell in adult midgut (Drosophila) and 64 other cell types or tissues


value: P/Homo B

value: domain

value: P/Homo B domain-containing protein

value: Membrane

value: Cytoplasmic vesicle

value: Neuroendocrine convertase 2

value: NEC 2

value: DMH #5

value: Prohormone convertase 2

value: Protein amontillado

value: amon

value: Serine endopeptidase which is involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues (PubMed:10749852, PubMed:20523747, PubMed:21138435). Required during embryonic and larval development, probably by proteolytically processing peptide hormones involved in hatching, larval growth and larval molting (PubMed:12586710). Required for the processing and activation of Akh which maintains normal hemolymph sugar levels (PubMed:20523747). Has been shown in one study to be required for processing of sli into slit N-product and slit C-product in the embryo which is necessary for lateral transverse muscle elongation but has been shown in another study not to be required for sli cleavage (PubMed:27628033, PubMed:32994163). Required for larval hatching (PubMed:10436051). Also required for normal larval wandering behavior which occurs prior to pupariation (PubMed:21138435). Required during pupal development for head eversion, leg and wing disk extension, and abdominal differentiation (PubMed:19559693). Required during eye development for R8 photoreceptor cell specification by regulating processing of ligands required for the BMP and activin signaling pathways (PubMed:28853393)

name: Release of protein hormones and neuropeptides from their precursors, generally by hydrolysis of -Lys-Arg-|- bonds.

value: Secreted

value: Expressed in the central nervous system (CNS) and midgut endocrine cells of third instar larva (at protein level) (PubMed:19559693). In the CNS, expressed in the CA-LP1 and CA-LP2 neurons which innervate the corpus allatum, and in the CC-MS2 neurons which innervate the corpora cardiaca of the ring gland (PubMed:19559693, PubMed:21138435). Also expressed in the CC-MS1, SP3, Tv and Va neurons (PubMed:21138435). Expressed in Akh-producing cells of the corpora cardiaca (PubMed:20523747). In the embryo, restricted to the final stages of embryogenesis where expression is found in anterior sensory structures and in only 168 cells in the brain and ventral nerve cord (PubMed:10436051). After larvae hatch, the sensory structures and most cells in the CNS turn off or substantially reduce expression (PubMed:10436051). In third instar larva, expressed at higher levels in the anterior section than in the posterior section (PubMed:10749852). Little expression is detected in the adult head (PubMed:10749852). In the developing eye, expressed at higher levels in pale-type R7 photoreceptor cells than in yellow-type R7 cells although expression is not seen in all pale-type R7 cells (PubMed:28853393). Also expressed in outer photoreceptor cells (PubMed:28853393)

commentType: TISSUE SPECIFICITY

value: Detected during embryogenesis, larval development, and pupal development (at protein level) (PubMed:10749852, PubMed:19559693). Also expressed in the adult (PubMed:10749852). In the embryo, first observed at 12-16 hours and peaks at the end of embryogenesis (PubMed:10436051). Levels drop in the first larval stage and reach a very low level by the second larval stage which continues throughout the remainder of larval and most of pupal life (PubMed:10436051). Expression increases in late pupae and peaks once again in adults (PubMed:10436051)

value: RNAi-mediated knockdown in Akh-producing cells of the corpora cardiaca results in a significant decrease in combined glucose and trehalose levels (PubMed:20523747). RNAi-mediated knockdown results in defective muscle patterning (PubMed:27628033). RNAi-mediated knockdown results in a reduction in the pale ommatidia subtype (PubMed:28853393)

value: Belongs to the peptidase S8 family. Furin subfamily

value: Has been shown in one study to be required for processing of sli into slit N-product and slit C-product (PubMed:27628033). However, has been shown in another study not to be required for sli cleavage with the protease required for sli cleavage being identified as tok (PubMed:27628033)

type: Propeptide

description: Neuroendocrine convertase 2

description: Peptidase S8

description: P/Homo B

type: Active site

description: Charge relay system

description: Charge relay system

description: Charge relay system

description: N-linked (GlcNAc...) asparagine

description: N-linked (GlcNAc...) asparagine

description: N-linked (GlcNAc...) asparagine

description: In amonC241Y: Partial embryonic lethality and defective larval growth with no pupariation or survival to adulthood. Defective Akh processing. Does not affect sli processing.

description: In amonC254Y: Partial embryonic lethality and defective larval growth with no pupariation or survival to adulthood.

description: In amonG367V: Partial embryonic lethality and defective larval growth with no pupariation or survival to adulthood.

description: In amonG473R: Partial embryonic lethality and defective larval growth with no pupariation or survival to adulthood.

description: In amonS557L1 and amonS557L2: Partial embryonic lethality and defective larval growth with no pupariation or survival to adulthood.

description: in Ref. 1; AAD49105

title: A role for amontillado, the Drosophila homolog of the neuropeptide precursor processing protease PC2, in triggering hatching behavior.

referencePositions:
NUCLEOTIDE SEQUENCE [MRNA]
FUNCTION
TISSUE SPECIFICITY
DEVELOPMENTAL STAGE

title: A Drosophila full-length cDNA resource.

authors:
Hwang J.R.
Siekhaus D.E.
Fuller R.S.
Taghert P.H.
Lindberg I.

title: Interaction of Drosophila melanogaster prohormone convertase 2 and 7B2. Insect cell-specific processing and secretion.

referencePositions:
FUNCTION
CATALYTIC ACTIVITY
SUBCELLULAR LOCATION
TISSUE SPECIFICITY
DEVELOPMENTAL STAGE

authors:
Rayburn L.Y.
Gooding H.C.
Choksi S.P.
Maloney D.
Kidd A.R. III
Siekhaus D.E.
Bender M.

title: amontillado, the Drosophila homolog of the prohormone processing protease PC2, is required during embryogenesis and early larval development.

referencePositions: MUTAGENESIS OF CYS-241; CYS-254; GLY-367; GLY-473 AND SER-557

authors:
Rayburn L.Y.
Rhea J.
Jocoy S.R.
Bender M.

title: The proprotein convertase amontillado (amon) is required during Drosophila pupal development.

title: The proprotein convertase encoded by amontillado (amon) is required in Drosophila corpora cardiaca endocrine cells producing the glucose regulatory hormone AKH.

referencePositions:
FUNCTION
TISSUE SPECIFICITY
DISRUPTION PHENOTYPE
MUTAGENESIS OF CYS-241

authors:
Wegener C.
Herbert H.
Kahnt J.
Bender M.
Rhea J.M.

title: Deficiency of prohormone convertase dPC2 (AMONTILLADO) results in impaired production of bioactive neuropeptide hormones in Drosophila.

title: Amontillado is required for Drosophila Slit processing and for tendon-mediated muscle patterning.

authors:
Wells B.S.
Pistillo D.
Barnhart E.
Desplan C.

title: Parallel Activin and BMP signaling coordinates R7/R8 photoreceptor subtype pairing in the stochastic Drosophila retina.

authors:
Kellermeyer R.
Heydman L.M.
Gillis T.
Mastick G.S.
Song M.
Kidd T.

title: Proteolytic cleavage of Slit by the Tolkin protease converts an axon repulsion cue to an axon growth cue in vivo.

value: Expressed in adult anterior midgut class I enteroendocrine cell in adult midgut (Drosophila) and 174 other cell types or tissues


value: Uncharacterized protein

value: keyword

value: Cystinosin

value: dCTNS

value: Ctns

value: Cystine/H(+) symporter that mediates export of cystine, the oxidized dimer of cysteine, from lysosomes (PubMed:35175796). Involved in cysteine homeostasis during periods of fasting, which indirectly regulates mTORC1-mediated signaling by supporting de novo CoA synthesis, the TCA cycle and amino acid metabolism during periods of food shortage (PubMed:35175796). Important for maintaining autophagy, and for development and survival during periods of fasting (PubMed:35175796)

name: L-cystine(out) + H(+)(out) = L-cystine(in) + H(+)(in)

directionType: left-to-right

value: Lysosome membrane

value: Multi-pass membrane protein

value: No effect on adult lifespan but larvae show a developmental delay (PubMed:35175796). The larval developmental delay is more severe when raised on a low-protein diet and adults die more quickly of starvation (PubMed:35175796). Accumulation of cystine in larvae (PubMed:35175796). Under fed conditions no difference in larval cysteine levels, however cysteine levels are reduced in fasting larvae (PubMed:35175796). Conditional RNAi-mediated knockdown in the fat body causes a developmental delay when on a low-protein diet (PubMed:35175796)

value: Belongs to the cystinosin family

description: Cystinosin

type: Topological domain

description: Lumenal

type: Transmembrane

description: Helical

description: Cytoplasmic

description: Helical

description: Lumenal

description: Helical

description: Cytoplasmic

description: Helical

description: Lumenal

description: Helical

description: Cytoplasmic

description: Helical

description: Lumenal

description: Helical

description: Cytoplasmic

description: PQ-loop 1

description: PQ-loop 2

description: Disordered

description: Polar residues

description: N-linked (GlcNAc...) asparagine

description: N-linked (GlcNAc...) asparagine

authors:
Stapleton M.
Carlson J.W.
Brokstein P.
Yu C.
Champe M.
George R.A.
Guarin H.
Kronmiller B.
Pacleb J.M.
Park S.
Wan K.H.
Rubin G.M.
Celniker S.E.

title: A Drosophila full-length cDNA resource.

authors:
Koles K.
Lim J.-M.
Aoki K.
Porterfield M.
Tiemeyer M.
Wells L.
Panin V.

title: Identification of N-glycosylated proteins from the central nervous system of Drosophila melanogaster.

referencePositions: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-86

authors:
Jouandin P.
Marelja Z.
Shih Y.H.
Parkhitko A.A.
Dambowsky M.
Asara J.M.
Nemazanyy I.
Dibble C.C.
Simons M.
Perrimon N.

title: Lysosomal cystine mobilization shapes the response of TORC1 and tissue growth to fasting.

value: the lysosomal cystine transporter (lct) family

value: Miscellaneous transport and binding events

value: Expressed in adult Malpighian tubule (Drosophila) and 42 other cell types or tissues

value: F:amino acid transmembrane transporter activity

value: F:L-cystine transmembrane transporter activity

value: P:L-cystine transmembrane transport from lysosomal lumen to cytosol


value: hydrated

value: Cytoplasm

value: rRNA methyltransferase 2, mitochondrial

value: rRNA (uridine-2'-O)-methyltransferase

value: S-adenosyl-L-methionine-dependent 2'-O-ribose methyltransferase that catalyzes the formation of 2'-O-methyluridine at position 1579 (Um1579) in the mitochondrial large subunit ribosomal RNA (mtLSU rRNA), a universally conserved modification in the peptidyl transferase domain of the mtLSU rRNA. This activity may require prior 2'-O-methylguanosine modification at position 1580 (Gm1580) by MRM3. Essential for late-stage assembly of mtLSU required for efficient translation of mitochondrial DNA encoded proteins; methyltransferase activity is not required for this function. Essential for mitochondrial respiratory function

name: a uridine in rRNA + S-adenosyl-L-methionine = a 2'-O-methyluridine in rRNA + S-adenosyl-L-homocysteine + H(+)

value: Mitochondrion

value: RNAi-mediated knock down is larval to late pupal lethal with developmental delay (PubMed:35177605). Escaper adults show wing deformation and flattened abdomen, and most are unable to emerge from pupae or get stuck in the food medium due to weakness (PubMed:35177605). Conditional muscle specific RNAi-mediated knockdown results in impaired locomotor ability (PubMed:35177605)

value: Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family

type: Transit peptide

description: Mitochondrion

type: Chain

description: rRNA methyltransferase 2, mitochondrial

description: Proton acceptor

type: Binding site

S-adenosyl-L-methionine

category: Ligand

database: PubMed

database: DOI

value: Berkeley

title: A Drosophila full-length cDNA resource.

value: Embryo

authors:
Rorbach J.
Boesch P.
Gammage P.A.
Nicholls T.J.
Pearce S.F.
Patel D.
Hauser A.
Perocchi F.
Minczuk M.

title: MRM2 and MRM3 are involved in biogenesis of the large subunit of the mitochondrial ribosome.

referencePositions: POSITION OF MODIFIED METHYLURIDINE IN MTLSU RRNA

authors:
Rebelo-Guiomar P.
Pellegrino S.
Dent K.C.
Sas-Chen A.
Miller-Fleming L.
Garone C.
Van Haute L.
Rogan J.F.
Dinan A.
Firth A.E.
Andrews B.
Whitworth A.J.
Schwartz S.
Warren A.J.
Minczuk M.

title: A late-stage assembly checkpoint of the human mitochondrial ribosome large subunit.

value: Expressed in adult anterior midgut class II enteroendocrine cell in adult midgut (Drosophila) and 58 other cell types or tissues

value: P:mitochondrial large ribosomal subunit assembly


value: Uncharacterized protein

value: Nucleus

value: Nucleolus

value: Ribosomal L1 domain-containing protein CG13096

value: Belongs to the universal ribosomal protein uL1 family. Highly divergent

description: Disordered

description: Disordered

description: Basic and acidic residues

description: Low complexity

description: Low complexity

description: Basic and acidic residues

description: Acidic residues

type: Modified residue

description: Phosphoserine

description: Phosphoserine

description: Phosphoserine

description: Phosphoserine

type: Sequence conflict

description: in Ref. 3; AAL39940

originalSequence: K

title: A Drosophila full-length cDNA resource.

title: An integrated chemical, mass spectrometric and computational strategy for (quantitative) phosphoproteomics: application to Drosophila melanogaster Kc167 cells.

journal: Mol. Biosyst.

referencePositions: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-89 AND SER-128

authors:
Zhai B.
Villen J.
Beausoleil S.A.
Mintseris J.
Gygi S.P.

title: Phosphoproteome analysis of Drosophila melanogaster embryos.

journal: J. Proteome Res.

referencePositions: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-15 AND SER-17

database: EMBL

value: Expressed in posterior terminal follicle cell in ovary and 115 other cell types or tissues

value: RIBOSOMAL L1 DOMAIN-CONTAINING PROTEIN CG13096


value: Glutaredoxin

value: domain

value: Glutaredoxin domain-containing protein

value: Glutaredoxin domain-containing cysteine-rich protein CG31559

value: Belongs to the GRXCR1 family

type: Domain

description: Glutaredoxin

description: Disordered

description: Disordered

description: Polar residues

description: Low complexity

description: Polar residues

description: Basic and acidic residues

title: The genome sequence of Drosophila melanogaster.

type: STRAIN

authors:
Stapleton M.
Brokstein P.
Hong L.
Agbayani A.
Carlson J.W.
Champe M.
Chavez C.
Dorsett V.
Dresnek D.
Farfan D.
Frise E.
George R.A.
Gonzalez M.
Guarin H.
Kronmiller B.
Li P.W.
Liao G.
Miranda A.
Mungall C.J.
Nunoo J.
Pacleb J.M.
Paragas V.
Park S.
Patel S.
Phouanenavong S.
Wan K.H.
Yu C.
Lewis S.E.
Rubin G.M.
Celniker S.E.

value: mRNA

database: RefSeq

database: AlphaFoldDB

database: SMR

database: BioGRID

database: FunCoup

database: IntAct

value: Expressed in dorsal appendage forming follicle cell in ovary and 11 other cell types or tissues

value: GLUTAREDOXIN DOMAIN-CONTAINING CYSTEINE-RICH PROTEIN 1


database: GO

value: F:translation release factor activity

value: GO

value: hydrated

value: Eukaryotic peptide chain release factor subunit 1

value: Eukaryotic release factor 1

value: Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA

value: Heterodimer of two subunits, one of which binds GTP

value: Cytoplasm

value: Belongs to the eukaryotic release factor 1 family

description: Phosphoserine

value: Berkeley

title: Annotation of the Drosophila melanogaster euchromatic genome: a systematic review.

title: A Drosophila full-length cDNA resource.

value: Embryo

referencePositions: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-341

value: ERF1-ERF3 translation termination complex

value: Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)

value: Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

value: Expressed in cleaving embryo and 217 other cell types or tissues

value: F:translation release factor activity, codon specific

value: N-terminal domain of eukaryotic peptide chain release factor subunit 1, ERF1


value: PANTHER

value: Tetratricopeptide repeat protein 36

value: Nucleus

value: P:otolith morphogenesis

value: Tetratricopeptide repeat protein 36 homolog

value: TPR repeat protein 36 homolog

value: Belongs to the TTC36 family

type: Repeat

description: TPR 1

description: TPR 2

description: TPR 3

authors:
Carlson J.W.
Booth B.
Frise E.
Sandler J.
Wan K.H.
Yu C.
Celniker S.E.

value: mRNA

database: STRING

database: PaxDb

database: PeptideAtlas

database: DNASU

database: EnsemblMetazoa

value: d. melanogaster

database: eggNOG

key: ToxonomicScope

value: Eukaryota

value: Expressed in epithelial cell in haltere and 27 other cell types or tissues


value: Uncharacterized protein

value: Endoplasmic reticulum membrane

pyridoxal 5'-phosphate

database: ChEBI

value: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family

value: Does not seem to possess aminotransferase activity

description: Alanine--glyoxylate aminotransferase 2-like

description: N6-(pyridoxal phosphate)lysine

value: Berkeley

title: A Drosophila full-length cDNA resource.

value: Embryo

database: GeneID

database: KEGG

database: UCSC

database: AGR

database: FlyBase

database: VEuPathDB

database: HOGENOM

database: InParanoid

database: OMA

value: Expressed in adult Malpighian tubule principal cell of lower segment in Malpighian tubule and 182 other cell types or tissues

value: ethanolamine-phosphate phospho-lyase isoform X1

value: Type I PLP-dependent aspartate aminotransferase-like (Major domain)


value: InterPro

value: hydrated

value: Organic solute transporter subunit alpha

value: keyword

value: Membrane

value: Organic solute transporter alpha-like protein

value: Solute carrier family 51 subunit alpha homolog

value: Probable transporter

value: Cell membrane

value: Multi-pass membrane protein

value: Belongs to the OST-alpha family

description: Extracellular

description: Helical

description: Cytoplasmic

description: Helical

description: Extracellular

description: Helical

description: Cytoplasmic

description: Helical

description: Extracellular

description: Helical

description: Cytoplasmic

description: Helical

description: Extracellular

description: Helical

description: Cytoplasmic

type: Glycosylation

description: N-linked (GlcNAc...) asparagine

description: N-linked (GlcNAc...) asparagine

description: N-linked (GlcNAc...) asparagine

description: N-linked (GlcNAc...) asparagine

description: N-linked (GlcNAc...) asparagine

description: in Ref. 3; AAL39357

originalSequence: I

database: PubMed

database: DOI

title: A Drosophila full-length cDNA resource.

value: Expressed in thoracico-abdominal ganglion (Drosophila) and 70 other cell types or tissues


value: Uncharacterized protein

database: GO

value: P:cell surface receptor signaling pathway

value: F:transmembrane signaling receptor activity

value: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G proteins. Required to coordinate the cytoskeletons of epidermal cells to produce a parallel array of cuticular hairs and bristles

value: Interacts with ATP6AP2

geneName: Grip

value: Expression starts at stage 6 in all cells between 15 and 70 per cent of egg length, including the invaginating cells of the ventral furrow. Stripe pattern is emerging by early stage 8. From stage 9 and continuing throughout embryogenesis, expression is seen in the developing CNS. At stage 10, expressed in 15 stripes in the presumptive head and trunk regions, in the posterior midgut primordium, in a subset of cells of anterior midgut invagination and in the procephalic lobe. At stage 12, expression declines in epidermis and increases in the midgut and visceral mesoderm. At stage 17, only expressed in the CNS, hindgut and dorsal vessel

commentType: DEVELOPMENTAL STAGE

value: Lys-Thr-X-X-X-Trp motif interacts with the PDZ domain of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway

value: The FZ domain is involved in binding with Wnt ligands

value: Belongs to the G protein-coupled receptor Fz/Smo family

type: Signal

description: Extracellular

description: Helical; Name=1

description: Cytoplasmic

description: Helical; Name=2

description: Extracellular

description: Helical; Name=3

description: Cytoplasmic

description: Helical; Name=4

description: Extracellular

description: Helical; Name=5

description: Cytoplasmic

description: Helical; Name=6

description: Extracellular

description: Helical; Name=7

description: Cytoplasmic

description: FZ

type: Region

description: Disordered

description: Lys-Thr-X-X-X-Trp motif, mediates interaction with the PDZ domain of Dvl family members

description: PDZ-binding

description: Gly residues

description: N-linked (GlcNAc...) asparagine

description: N-linked (GlcNAc...) asparagine

description: in Ref. 1; AAC47273

description: in Ref. 1; AAC47273

authors:
Bhanot P.
Brink M.
Samos C.H.
Hsieh J.C.
Wang Y.
Macke J.P.
Andrew D.
Nathans J.
Nusse R.

title: A new member of the frizzled family from Drosophila functions as a Wingless receptor.

referencePositions:
NUCLEOTIDE SEQUENCE [GENOMIC DNA]
INTERACTION WITH WG THROUGH FZ DOMAIN

authors:
Hsieh J.C.
Rattner A.
Smallwood P.M.
Nathans J.

title: Biochemical characterization of Wnt-frizzled interactions using a soluble, biologically active vertebrate Wnt protein.

authors:
Stapleton M.
Carlson J.W.
Chavez C.
Frise E.
George R.A.
Pacleb J.M.
Park S.
Wan K.H.
Yu C.
Celniker S.E.

authors:
Buechling T.
Bartscherer K.
Ohkawara B.
Chaudhary V.
Spirohn K.
Niehrs C.
Boutros M.

title: Wnt/Frizzled signaling requires dPRR, the Drosophila homolog of the prorenin receptor.

value: the g-protein-coupled receptor (gpcr) family

value: Recruitment of the 'destruction complex' to the receptor complex, the degradation of AXN and release of ARM

value: WNT5A-dependent internalization of FZD2, FZD5 and ROR2

value: Expressed in T neuron T5d (Drosophila) in embryonic/larval optic lobe (Drosophila) and 296 other cell types or tissues

value: P:chitin-based larval cuticle pattern formation

value: P:imaginal disc-derived wing margin morphogenesis

value: Rhodopsin 7-helix transmembrane proteins


value: Sm

value: domain

value: Sm domain-containing protein

value: Probable small nuclear ribonucleoprotein G

value: snRNP-G

value: Sm protein G

value: Sm-G

value: SmG

value: SNRPG

value: Plays a role in pre-mRNA splicing as a core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome (PubMed:18621711). Component of both the pre-catalytic spliceosome B complex and activated spliceosome C complexes. Is also a component of the minor U12 spliceosome (By similarity)

value: Interacts with the SMN complex (PubMed:18621711). Core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Most spliceosomal snRNPs contain a common set of Sm proteins, SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. Component of the U1 snRNP. Component of the U4/U6-U5 tri-snRNP complex. Component of the U7 snRNP complex. Component of the U11/U12 snRNPs that are part of the U12-type spliceosome (By similarity)

geneName: sals

value: SMN-mediated assembly into core snRNPs occurs in the cytosol before SMN-mediated transport to the nucleus to be included in spliceosomes

value: Cytoplasm, cytosol

value: Nucleus

value: Belongs to the snRNP Sm proteins family

commentType: SIMILARITY

note: Wrong choice of frame.

description: Sm

authors:
Adams M.D.
Celniker S.E.
Holt R.A.
Evans C.A.
Gocayne J.D.
Amanatides P.G.
Scherer S.E.
Li P.W.
Hoskins R.A.
Galle R.F.
George R.A.
Lewis S.E.
Richards S.
Ashburner M.
Henderson S.N.
Sutton G.G.
Wortman J.R.
Yandell M.D.
Zhang Q.
Chen L.X.
Brandon R.C.
Rogers Y.-H.C.
Blazej R.G.
Champe M.
Pfeiffer B.D.
Wan K.H.
Doyle C.
Baxter E.G.
Helt G.
Nelson C.R.
Miklos G.L.G.
Abril J.F.
Agbayani A.
An H.-J.
Andrews-Pfannkoch C.
Baldwin D.
Ballew R.M.
Basu A.
Baxendale J.
Bayraktaroglu L.
Beasley E.M.
Beeson K.Y.
Benos P.V.
Berman B.P.
Bhandari D.
Bolshakov S.
Borkova D.
Botchan M.R.
Bouck J.
Brokstein P.
Brottier P.
Burtis K.C.
Busam D.A.
Butler H.
Cadieu E.
Center A.
Chandra I.
Cherry J.M.
Cawley S.
Dahlke C.
Davenport L.B.
Davies P.
de Pablos B.
Delcher A.
Deng Z.
Mays A.D.
Dew I.
Dietz S.M.
Dodson K.
Doup L.E.
Downes M.
Dugan-Rocha S.
Dunkov B.C.
Dunn P.
Durbin K.J.
Evangelista C.C.
Ferraz C.
Ferriera S.
Fleischmann W.
Fosler C.
Gabrielian A.E.
Garg N.S.
Gelbart W.M.
Glasser K.
Glodek A.
Gong F.
Gorrell J.H.
Gu Z.
Guan P.
Harris M.
Harris N.L.
Harvey D.A.
Heiman T.J.
Hernandez J.R.
Houck J.
Hostin D.
Houston K.A.
Howland T.J.
Wei M.-H.
Ibegwam C.
Jalali M.
Kalush F.
Karpen G.H.
Ke Z.
Kennison J.A.
Ketchum K.A.
Kimmel B.E.
Kodira C.D.
Kraft C.L.
Kravitz S.
Kulp D.
Lai Z.
Lasko P.
Lei Y.
Levitsky A.A.
Li J.H.
Li Z.
Liang Y.
Lin X.
Liu X.
Mattei B.
McIntosh T.C.
McLeod M.P.
McPherson D.
Merkulov G.
Milshina N.V.
Mobarry C.
Morris J.
Moshrefi A.
Mount S.M.
Moy M.
Murphy B.
Murphy L.
Muzny D.M.
Nelson D.L.
Nelson D.R.
Nelson K.A.
Nixon K.
Nusskern D.R.
Pacleb J.M.
Palazzolo M.
Pittman G.S.
Pan S.
Pollard J.
Puri V.
Reese M.G.
Reinert K.
Remington K.
Saunders R.D.C.
Scheeler F.
Shen H.
Shue B.C.
Siden-Kiamos I.
Simpson M.
Skupski M.P.
Smith T.J.
Spier E.
Spradling A.C.
Stapleton M.
Strong R.
Sun E.
Svirskas R.
Tector C.
Turner R.
Venter E.
Wang A.H.
Wang X.
Wang Z.-Y.
Wassarman D.A.
Weinstock G.M.
Weissenbach J.
Williams S.M.
Woodage T.
Worley K.C.
Wu D.
Yang S.
Yao Q.A.
Ye J.
Yeh R.-F.
Zaveri J.S.
Zhan M.
Zhang G.
Zhao Q.
Zheng L.
Zheng X.H.
Zhong F.N.
Zhong W.
Zhou X.
Zhu S.C.
Zhu X.
Smith H.O.
Gibbs R.A.
Myers E.W.
Rubin G.M.
Venter J.C.

value: Berkeley

authors:
Misra S.
Crosby M.A.
Mungall C.J.
Matthews B.B.
Campbell K.S.
Hradecky P.
Huang Y.
Kaminker J.S.
Millburn G.H.
Prochnik S.E.
Smith C.D.
Tupy J.L.
Whitfield E.J.
Bayraktaroglu L.
Berman B.P.
Bettencourt B.R.
Celniker S.E.
de Grey A.D.N.J.
Drysdale R.A.
Harris N.L.
Richter J.
Russo S.
Schroeder A.J.
Shu S.Q.
Stapleton M.
Yamada C.
Ashburner M.
Gelbart W.M.
Rubin G.M.
Lewis S.E.

title: A Drosophila full-length cDNA resource.

value: Embryo

value: Head

authors:
Mount S.M.
Salz H.K.

title: Pre-messenger RNA processing factors in the Drosophila genome.

journal: J. Cell Biol.

authors:
Kroiss M.
Schultz J.
Wiesner J.
Chari A.
Sickmann A.
Fischer U.

title: Evolution of an RNP assembly system: a minimal SMN complex facilitates formation of UsnRNPs in Drosophila melanogaster.

referencePositions:
FUNCTION
INTERACTION WITH THE SMN COMPLEX

database: EMBL

value: mRNA

value: U7 small nuclear ribonucleoprotein complex

value: SLBP independent Processing of Histone Pre-mRNAs

value: RNA Polymerase II Transcription Termination

value: SLBP Dependent Processing of Replication-Dependent Histone Pre-mRNAs

value: Expressed in eye disc (Drosophila) and 166 other cell types or tissues

value: C:small nuclear ribonucleoprotein complex

value: P:nuclear histone mRNA catabolic process


value: Uncharacterized protein

value: Gem-associated protein 4a

value: Protein Gaulos

value: Glos

value: Component of the survival motor neuron (SMN) complex that catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs (PubMed:28949413, PubMed:30563832). One of 3 almost identical paralogs (Glos/Gem4a, Gem4b and Gem4c), resulting from a genomic triplication, that have some redundant function (PubMed:30563832). Required for neuromuscular function and organismal viability (PubMed:28949413, PubMed:30563832)

value: Component of the core survival motor neuron (SMN) complex composed of Smn, Gem2, Gem3, rig/Gem5 and one of 3 almost identical Gem4 paralogs encoded by Glos/Gem4a, Gem4b or Gem4c (PubMed:30563832). Interacts with Smn; the interaction is probably indirect (PubMed:28949413, PubMed:30563832)

value: RNAi-mediated knockdown is pupal to adult lethal while simultaneous RNAi-mediated knockdown of all 3 Gem4 paralogs (Glos/Gem4a, Gem4b and Gem4c) is larval lethal; this phenotype does not appear to be due to loss of snRNP biogenesis (PubMed:28949413, PubMed:30563832, PubMed:35861185). Conditional RNAi-mediated knockdown in muscle cells or the central nervous system reduces adult viability and results in age-dependent progressive decline of the neuromuscular system; knockdown in muscle cells becomes fully adult lethal due to reduced motor function when combined with a hypomorphic Gem3 mutation (PubMed:28949413). Conditional RNAi-mediated knockdown in adults results in a reduction in climbing ability and lifespan; adults have decreased numbers of mature boutons and increased numbers of satellite boutons at the neuromuscular junction (PubMed:35861185)

value: 'Gaulos' is the Phoenician word for a merchant boat. This name reflects the boat shaped expression pattern seen in stage 7-10 embryos

description: Gem-associated protein 4a

title: A Drosophila full-length cDNA resource.

authors:
Lanfranco M.
Cacciottolo R.
Borg R.M.
Vassallo N.
Juge F.
Bordonne R.
Cauchi R.J.

title: Novel interactors of the Drosophila Survival Motor Neuron (SMN) Complex suggest its full conservation.

journal: FEBS Lett.

referencePositions:
FUNCTION
INTERACTION WITH SMN
DISRUPTION PHENOTYPE
NOMENCLATURE

authors:
Matera A.G.
Raimer A.C.
Schmidt C.A.
Kelly J.A.
Droby G.N.
Baillat D.
Ten Have S.
Lamond A.I.
Wagner E.J.
Gray K.M.

title: Composition of the Survival Motor Neuron (SMN) Complex in Drosophila melanogaster.

journal: G3 (Bethesda)

referencePositions:
FUNCTION
IDENTIFICATION IN THE SMN COMPLEX
INTERACTION WITH SMN
DISRUPTION PHENOTYPE
IDENTIFICATION BY MASS SPECTROMETRY
NOMENCLATURE

authors:
Altassan R.
Qudair A.
Alokaili R.
Alhasan K.
Faqeih E.A.
Alhashem A.
Alowain M.
Alsayed M.
Rahbeeni Z.
Albadi L.
Alkuraya F.S.
Anderson E.N.
Rajan D.
Pandey U.B.

title: Further delineation of GEMIN4 related neurodevelopmental disorder with microcephaly, cataract, and renal abnormalities syndrome.

journal: Am. J. Med. Genet. A

database: RefSeq

database: AlphaFoldDB

database: SMR

value: Survival motor neuron complex, Gem4A variant

value: Expressed in cleaving embryo and 51 other cell types or tissues


value: cAMP-specific 3',5'-cyclic phosphodiesterase, isoforms N/G

value: substring

value: Phosphodiesterase

value: GO

value: Cytoplasm

value: hydrated

value: 3',5'-cyclic-AMP phosphodiesterase, isoforms N/G

value: Learning/memory process protein

value: Protein dunce

value: cAMP-specific phosphodiesterase, isoforms N/G

value: dnc

value: Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes (By similarity). Vital for female fertility. Required for learning/memory (By similarity)

3',5'-cyclic AMP + H2O = AMP + H(+)

database: Rhea

database: ChEBI

value: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.

value: Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1

value: Monomer

events:
Alternative splicing
Alternative initiation

value: N

value: G

value: F

isoformSequenceStatus: External

value: I

value: B

value: S

value: II

value: J

value: III

value: E

value: P

value: IV

value: A

value: V

value: C

value: VI

value: D

value: VII

value: L

value: R

value: Q

value: U

value: T

value: Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily

commentType: SEQUENCE CAUTION

description: PDEase

description: Disordered

description: Disordered

description: Disordered

description: Disordered

description: Disordered

description: Basic and acidic residues

description: Polar residues

description: Gly residues

description: Low complexity

description: Low complexity

description: Acidic residues

description: Low complexity

description: Low complexity

description: Polar residues

description: Polar residues

description: Polar residues

description: Acidic residues

description: Low complexity

description: Gly residues

description: Polar residues

description: Proton donor

description: Binds AMP, but not cAMP

description: in isoform G

referencePositions:
GENOME REANNOTATION
ALTERNATIVE SPLICING

authors:
Benos P.V.
Gatt M.K.
Ashburner M.
Murphy L.
Harris D.
Barrell B.G.
Ferraz C.
Vidal S.
Brun C.
Demailles J.
Cadieu E.
Dreano S.
Gloux S.
Lelaure V.
Mottier S.
Galibert F.
Borkova D.
Minana B.
Kafatos F.C.
Louis C.
Siden-Kiamos I.
Bolshakov S.
Papagiannakis G.
Spanos L.
Cox S.
Madueno E.
de Pablos B.
Modolell J.
Peter A.
Schoettler P.
Werner M.
Mourkioti F.
Beinert N.
Dowe G.
Schaefer U.
Jaeckle H.
Bucheton A.
Callister D.M.
Campbell L.A.
Darlamitsou A.
Henderson N.S.
McMillan P.J.
Salles C.
Tait E.A.
Valenti P.
Saunders R.D.C.
Glover D.M.

title: From sequence to chromosome: the tip of the X chromosome of D. melanogaster.

referencePositions: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 170-479

value: Expressed in muscle cell in antenna and 285 other cell types or tissues

value: 3'5'-cyclic nucleotide phosphodiesterase, catalytic domain


value: Uncharacterized protein

value: Plays a role in DNA repair. May be a component of an E3 ubiquitin-protein ligase which promotes histone ubiquitination in response to UV irradiation. Histone ubiquitination may be important for subsequent DNA repair

value: F:nucleic acid binding

value: keyword

value: hydrated

value: DNA damage-binding protein 1

value: Damage-specific DNA-binding protein 1

value: Protein piccolo

value: pic

value: Protein, which is both involved in DNA repair and protein ubiquitination, as part of the UV-DDB complex and DCX (DDB1-CUL4-X-box) complexes, respectively (By similarity). Core component of the UV-DDB complex (UV-damaged DNA-binding protein complex), a complex that recognizes UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair (By similarity). The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches (By similarity). Also functions as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity). The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1 (By similarity). Required for degradation of gig (PubMed:18381890). Required for genomic stability in the face of endogenous DNA lesions and for the response to MMS-induced DNA damage (PubMed:16428319). Required for normal wing development (PubMed:16428319)

value: Protein modification; protein ubiquitination

value: Component of the UV-DDB complex which includes DDB1 and DDB2; the heterodimer dimerizes to give rise to a heterotetramer when bound to damaged DNA (By similarity). The UV-DDB complex interacts with monoubiquitinated histone H2A and binds to XPC via the DDB2 subunit (By similarity). Component of numerous DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which consist of a core of DDB1, CUL4A or CUL4B and RBX1 (By similarity). DDB1 may recruit specific substrate targeting subunits to the DCX complex (By similarity). These substrate targeting subunits are generally known as DCAF (DDB1- and CUL4-associated factor) or CDW (CUL4-DDB1-associated WD40-repeat) proteins (By similarity). Interacts with Fbw5 and gig (PubMed:18381890). May interact with ohgt (PubMed:27702999)

commentType: SUBUNIT

value: Cytoplasm

value: Nucleus

value: Reduced tissue growth in the wing imaginal disk

value: Belongs to the DDB1 family

description: DNA damage-binding protein 1

value: Berkeley

authors:
Rubin G.M.
Hong L.
Brokstein P.
Evans-Holm M.
Frise E.
Stapleton M.
Harvey D.A.

title: A Drosophila complementary DNA resource.

value: Embryo

authors:
Shimanouchi K.
Takata K.
Yamaguchi M.
Murakami S.
Ishikawa G.
Takeuchi R.
Kanai Y.
Ruike T.
Nakamura R.
Abe Y.
Sakaguchi K.

title: Drosophila damaged DNA binding protein 1 contributes to genome stability in somatic cells.

journal: J. Biochem.

referencePositions:
FUNCTION
DISRUPTION PHENOTYPE

authors:
Hu J.
Zacharek S.
He Y.J.
Lee H.
Shumway S.
Duronio R.J.
Xiong Y.

title: WD40 protein FBW5 promotes ubiquitination of tumor suppressor TSC2 by DDB1-CUL4-ROC1 ligase.

journal: Genes Dev.

referencePositions:
FUNCTION
INTERACTION WITH FBW5 AND GIG

authors:
Wakabayashi S.
Sawamura N.
Voelzmann A.
Broemer M.
Asahi T.
Hoch M.

title: Ohgata, the single Drosophila ortholog of Human Cereblon, regulates insulin signaling-dependent organismic growth.

journal: J. Biol. Chem.

value: mRNA

database: BioGRID

database: FunCoup

value: Recognition of DNA damage by PCNA-containing replication complex

value: Formation of TC-NER Pre-Incision Complex

value: Gap-filling DNA repair synthesis and ligation in TC-NER

value: Expressed in eye disc (Drosophila) and 100 other cell types or tissues

value: P:negative regulation of smoothened signaling pathway

value: P:positive regulation of protein catabolic process

value: P:proteasome-mediated ubiquitin-dependent protein catabolic process

value: P:ubiquitin-dependent protein catabolic process

value: YVTN repeat-like/Quinoprotein amine dehydrogenase