organism gene review_path gene_symbol uniprot_id taxon_id taxon_label annotation_index reactome_id reactome_title reactome_cached reactome_path reactome_display_name reactome_species term_id term_label evidence_type qualifier aspect negated isoform review_action review_summary candidate_verdict candidate_bucket manual_verdict manual_bucket manual_reason candidate_flags genes_for_reactome_id matching_module_mfs DROME Akt1 genes/DROME/Akt1/Akt1-ai-review.yaml Akt1 Q8INB9 NCBITaxon:7227 Drosophila melanogaster 20 R-DME-110475 DAkt1-mediated phosphorylation inactivates DFOXO yes reactome/R-DME-110475.md DAkt1-mediated phosphorylation inactivates DFOXO Drosophila melanogaster GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Akt1 is a serine/threonine protein kinase. reaction_mf_exact accepted_mf 1 DROME Akt1 genes/DROME/Akt1/Akt1-ai-review.yaml Akt1 Q8INB9 NCBITaxon:7227 Drosophila melanogaster 21 R-DME-169828 Activated DAkt1 phosphorylates TSC2 which inhibits the TSC1/2 complex yes reactome/R-DME-169828.md Activated DAkt1 phosphorylates TSC2 which inhibits the TSC1/2 complex Drosophila melanogaster GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Akt1 is a serine/threonine protein kinase. reaction_mf_exact accepted_mf 1 DROME Pdk1 genes/DROME/Pdk1/Pdk1-ai-review.yaml Pdk1 Q9W0V1 NCBITaxon:7227 Drosophila melanogaster 17 R-DME-110468 Activation of DAkt1 by DPDK1 yes reactome/R-DME-110468.md Activation of DAkt1 by DPDK1 Drosophila melanogaster GO:0004676 3-phosphoinositide-dependent protein kinase activity TAS enables MF false ACCEPT PDK1 has 3-phosphoinositide-dependent protein kinase activity. reaction_mf_exact accepted_mf 1 DROME Pdk1 genes/DROME/Pdk1/Pdk1-ai-review.yaml Pdk1 Q9W0V1 NCBITaxon:7227 Drosophila melanogaster 18 R-DME-169827 DPDK1 phosphorylates and activates DS6K yes reactome/R-DME-169827.md DPDK1 phosphorylates and activates DS6K Drosophila melanogaster GO:0004676 3-phosphoinositide-dependent protein kinase activity TAS enables MF false ACCEPT PDK1 has 3-phosphoinositide-dependent protein kinase activity. reaction_mf_exact accepted_mf 1 SCHPO chk1 genes/SCHPO/chk1/chk1-ai-review.yaml chk1 P34208 NCBITaxon:284812 Schizosaccharomyces pombe (strain 972 / ATCC 24843) 14 R-SPO-75040 Phosphorylation of Wee1 kinase by Chk1 yes reactome/R-SPO-75040.md Phosphorylation of Wee1 kinase by Chk1 Schizosaccharomyces pombe GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Reactome annotation (Phosphorylation of Wee1 kinase by Chk1) supporting Chk1's Ser/Thr kinase activity. Correct core molecular function. reaction_mf_exact accepted_mf 1 human A4GALT genes/human/A4GALT/A4GALT-ai-review.yaml A4GALT Q9NPC4 NCBITaxon:9606 Homo sapiens 7 R-HSA-9846477 A4GALT transfers galactose to LacCer yes reactome/R-HSA-9846477.md A4GALT transfers galactose to LacCer Homo sapiens GO:0050512 lactosylceramide 4-alpha-galactosyltransferase activity TAS enables MF false ACCEPT "Reactome TAS annotation (reaction ""A4GALT transfers galactose to LacCer"") to the specific molecular function lactosylceramide 4-alpha-galactosyltransferase activity. This is the enzyme's core catalytic function and matches the experimental evidence." reaction_mf_exact accepted_mf 1 human A4GNT genes/human/A4GNT/A4GNT-ai-review.yaml A4GNT Q9UNA3 NCBITaxon:9606 Homo sapiens 10 R-HSA-5694487 A4GNT transfers GlcNAc to core 2 mucins yes reactome/R-HSA-5694487.md A4GNT transfers GlcNAc to core 2 mucins Homo sapiens GO:0008375 acetylglucosaminyltransferase activity TAS enables MF false ACCEPT Reactome assigns A4GNT the transfer of GlcNAc to core 2 mucins, creating an alpha-1,4 linkage to beta-galactose residues. reaction_mf_exact accepted_mf 1 human AACS genes/human/AACS/AACS-ai-review.yaml AACS Q86V21 NCBITaxon:9606 Homo sapiens 8 R-HSA-5694494 AACS ligates CoA-SH to ACA, forming ACA-CoA yes reactome/R-HSA-5694494.md AACS ligates CoA-SH to ACA, forming ACA-CoA Homo sapiens GO:0030729 acetoacetate-CoA ligase activity TAS enables MF false ACCEPT The human Reactome event states that AACS ligates CoA to acetoacetate to form acetoacetyl-CoA in the cytosol, which is precisely GO:0030729. reaction_mf_exact accepted_mf 1 human AADAC genes/human/AADAC/AADAC-ai-review.yaml AADAC P22760 NCBITaxon:9606 Homo sapiens 33 R-HSA-5689000 AADAC deacetylates PHEN yes reactome/R-HSA-5689000.md AADAC deacetylates PHEN Homo sapiens GO:0019213 deacetylase activity TAS enables MF false ACCEPT The Reactome reaction assigns AADAC-catalyzed phenacetin deacetylation to p-phenetidine, a well-established human AADAC drug-hydrolysis activity. reaction_mf_exact accepted_mf 1 human AAK1 genes/human/AAK1/AAK1-ai-review.yaml AAK1 Q2M2I8 NCBITaxon:9606 Homo sapiens 14 R-HSA-8856813 AAK1 phosphorylates AP-2 mu subunit at T156 yes reactome/R-HSA-8856813.md AAK1 phosphorylates AP-2 mu subunit at T156 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Reactome represents AAK1 phosphorylating the AP-2 mu subunit and maps the event to protein serine/threonine kinase activity. This matches the core catalytic function established experimentally. reaction_mf_exact accepted_mf 1 human AANAT genes/human/AANAT/AANAT-ai-review.yaml AANAT Q16613 NCBITaxon:9606 Homo sapiens 27 R-HSA-209792 N-acetylation of serotonin yes reactome/R-HSA-209792.md N-acetylation of serotonin Homo sapiens GO:0004059 aralkylamine N-acetyltransferase activity TAS enables MF false ACCEPT Reactome TAS assertion of the core aralkylamine N-acetyltransferase activity (N-acetylation of serotonin). Correct and consistent with experimental and phylogenetic evidence; accepted as the gene's core molecular function. reaction_mf_exact accepted_mf 1 human AARS1 genes/human/AARS1/AARS1-ai-review.yaml AARS1 P49588 NCBITaxon:9606 Homo sapiens 23 R-HSA-379864 alanine + tRNA(Ala) + ATP => Ala-tRNA(Ala) + AMP + pyrophosphate yes reactome/R-HSA-379864.md alanine + tRNA(Ala) + ATP => Ala-tRNA(Ala) + AMP + pyrophosphate Homo sapiens GO:0004813 alanine-tRNA ligase activity TAS enables MF false ACCEPT Reactome assigns alanine-tRNA ligase activity to AARS1 in the cytosolic Ala-tRNA(Ala) reaction. reaction_mf_exact accepted_mf 1 human AASDHPPT genes/human/AASDHPPT/AASDHPPT-ai-review.yaml AASDHPPT Q9NRN7 NCBITaxon:9606 Homo sapiens 14 R-HSA-199202 Phosphopantetheine conjugation of the ACP domain of FAS yes reactome/R-HSA-199202.md Phosphopantetheine conjugation of the ACP domain of FAS Homo sapiens GO:0008897 holo-[acyl-carrier-protein] synthase activity TAS enables MF false ACCEPT Reactome explicitly assigns AASDHPPT the transfer of phosphopantetheine from CoA to the ACP domain of FASN, matching the GO molecular function. reaction_mf_exact accepted_mf 1 human ABCA3 genes/human/ABCA3/ABCA3-ai-review.yaml ABCA3 Q99758 NCBITaxon:9606 Homo sapiens 2 R-HSA-5683672 Defective ABCA3 does not transport PC, PG from ER membrane to lamellar body yes reactome/R-HSA-5683672.md Defective ABCA3 does not transport PC, PG from ER membrane to lamellar body Homo sapiens GO:0005319 lipid carrier activity TAS enables MF false ACCEPT ABCA3 is an ATP-dependent membrane lipid carrier. reaction_mf_exact accepted_mf reactome_disease_event 1 human ABCA3 genes/human/ABCA3/ABCA3-ai-review.yaml ABCA3 Q99758 NCBITaxon:9606 Homo sapiens 3 R-HSA-5683714 ABCA3 transports PC, PG from ER membrane to lamellar body yes reactome/R-HSA-5683714.md ABCA3 transports PC, PG from ER membrane to lamellar body Homo sapiens GO:0005319 lipid carrier activity TAS enables MF false ACCEPT ABCA3 is an ATP-dependent membrane lipid carrier. reaction_mf_exact accepted_mf 1 human ABCA3 genes/human/ABCA3/ABCA3-ai-review.yaml ABCA3 Q99758 NCBITaxon:9606 Homo sapiens 4 R-HSA-5688397 Defective ABCA3 does not transport PC, PG from ER membrane to lamellar body yes reactome/R-HSA-5688397.md Defective ABCA3 does not transport PC, PG from ER membrane to lamellar body Homo sapiens GO:0005319 lipid carrier activity TAS enables MF false ACCEPT ABCA3 is an ATP-dependent membrane lipid carrier. reaction_mf_exact accepted_mf reactome_disease_event 1 human ABCA7 genes/human/ABCA7/ABCA7-ai-review.yaml ABCA7 Q8IZY2 NCBITaxon:9606 Homo sapiens 33 R-HSA-382553 ABCA7:Apo1A-mediated phospholipid efflux yes reactome/R-HSA-382553.md ABCA7:Apo1A-mediated phospholipid efflux Homo sapiens GO:0120014 phospholipid transfer activity TAS enables MF false ACCEPT This molecular-function annotation captures the core ABCA7 activity: ATP-coupled intramembrane phospholipid transport/floppase activity and apoA-I-associated phospholipid efflux. reaction_mf_exact accepted_mf 1 human ABCC6 genes/human/ABCC6/ABCC6-ai-review.yaml ABCC6 O95255 NCBITaxon:9606 Homo sapiens 54 R-HSA-1454916 The ABCC family mediates organic anion transport yes reactome/R-HSA-1454916.md The ABCC family mediates organic anion transport Homo sapiens GO:0042626 ATPase-coupled transmembrane transporter activity TAS enables MF false ACCEPT ABCC6 is an ATPase-coupled primary active membrane transporter. reaction_mf_exact accepted_mf multi_gene_reactome_id 3 human ABCC6 genes/human/ABCC6/ABCC6-ai-review.yaml ABCC6 O95255 NCBITaxon:9606 Homo sapiens 55 R-HSA-5690340 Defective ABCC6 does not transport organic anion from cytosol to extracellular region yes reactome/R-HSA-5690340.md Defective ABCC6 does not transport organic anion from cytosol to extracellular region Homo sapiens GO:0042626 ATPase-coupled transmembrane transporter activity TAS enables MF false ACCEPT ABCC6 is an ATPase-coupled primary active membrane transporter. reaction_mf_exact accepted_mf reactome_disease_event 1 human ABCC8 genes/human/ABCC8/ABCC8-ai-review.yaml ABCC8 Q09428 NCBITaxon:9606 Homo sapiens 6 R-HSA-265682 KCNJ11 tetramer:ABCC8 tetramer binds 4xATP, closing the channel yes reactome/R-HSA-265682.md KCNJ11 tetramer:ABCC8 tetramer binds 4xATP, closing the channel Homo sapiens GO:0005524 ATP binding TAS enables MF false ACCEPT SUR1 directly binds ATP in its ABC nucleotide-binding domains. reaction_mf_exact accepted_mf 1 human ABCE1 genes/human/ABCE1/ABCE1-ai-review.yaml ABCE1 P61221 NCBITaxon:9606 Homo sapiens 18 R-HSA-9955731 ABCE1:PELO:80S Ribosome:non-stop mRNA:peptidyl-tRNA with elongating peptide dissociates yielding ABCE1:40S ribosomal subunit, PELO, and 60S ribosomal subunit:peptidyl-tRNA yes reactome/R-HSA-9955731.md ABCE1:PELO:80S Ribosome:non-stop mRNA:peptidyl-tRNA with elongating peptide dissociates yielding ABCE1:40S ribosomal subunit, PELO, and 60S ribosomal subunit:peptidyl-tRNA with nascent peptide Homo sapiens GO:0016887 ATP hydrolysis activity TAS enables MF false ACCEPT Reactome explicitly describes ABCE1 hydrolyzing ATP while bound with PELO to split a stalled 80S ribosome. reaction_mf_exact accepted_mf 1 human ABL1 genes/human/ABL1/ABL1-ai-review.yaml ABL1 P00519 NCBITaxon:9606 Homo sapiens 226 R-HSA-428888 Reactome - ESR-mediated signaling yes reactome/R-HSA-428888.md Phosphorylation of ROBO1 by ABL kinase Homo sapiens GO:0004713 protein tyrosine kinase activity TAS enables MF false ACCEPT ABL1 tyrosine kinase activity in Reactome pathway. reaction_mf_exact accepted_mf 1 human ABL1 genes/human/ABL1/ABL1-ai-review.yaml ABL1 P00519 NCBITaxon:9606 Homo sapiens 227 R-HSA-5686587 Reactome - Lysosome yes reactome/R-HSA-5686587.md ABL1 phosphorylates RAD52 Homo sapiens GO:0004713 protein tyrosine kinase activity TAS enables MF false ACCEPT ABL1 tyrosine kinase activity in Reactome pathway. reaction_mf_exact accepted_mf 1 human ABL1 genes/human/ABL1/ABL1-ai-review.yaml ABL1 P00519 NCBITaxon:9606 Homo sapiens 228 R-HSA-8956659 Reactome - ABL1 signaling events yes reactome/R-HSA-8956659.md ABL1 phosphorylates YAP1 Homo sapiens GO:0004713 protein tyrosine kinase activity TAS enables MF false ACCEPT ABL1 tyrosine kinase activity in Reactome pathway. reaction_mf_exact accepted_mf 1 human ABL1 genes/human/ABL1/ABL1-ai-review.yaml ABL1 P00519 NCBITaxon:9606 Homo sapiens 229 R-HSA-9841924 Reactome - ABL1 signaling in DNA damage response yes reactome/R-HSA-9841924.md ABL1 phosphorylates PPARG2 Homo sapiens GO:0004713 protein tyrosine kinase activity TAS enables MF false ACCEPT ABL1 tyrosine kinase activity. reaction_mf_exact accepted_mf 1 human ABL1 genes/human/ABL1/ABL1-ai-review.yaml ABL1 P00519 NCBITaxon:9606 Homo sapiens 230 R-HSA-9865196 Reactome - ABL1 kinase activity yes reactome/R-HSA-9865196.md p-Y393-ABL1 phosphorylates tyrosine-407 of YAP1 Homo sapiens GO:0004713 protein tyrosine kinase activity TAS enables MF false ACCEPT ABL1 tyrosine kinase activity. reaction_mf_exact accepted_mf 1 human ABR genes/human/ABR/ABR-ai-review.yaml ABR Q12979 NCBITaxon:9606 Homo sapiens 17 R-HSA-9014296 RAC2 GEFs activate RAC2 yes reactome/R-HSA-9014296.md RAC2 GEFs activate RAC2 Homo sapiens GO:0005085 guanyl-nucleotide exchange factor activity TAS enables MF false ACCEPT Traceable Reactome statement crediting the primary GEF biochemistry. reaction_mf_exact accepted_mf 1 human ABR genes/human/ABR/ABR-ai-review.yaml ABR Q12979 NCBITaxon:9606 Homo sapiens 18 R-HSA-9013022 RHOB GAPs stimulate RHOB GTPase activity yes reactome/R-HSA-9013022.md RHOB GAPs stimulate RHOB GTPase activity Homo sapiens GO:0005096 GTPase activator activity TAS enables MF false ACCEPT Correct term. The RHOB substrate assignment behind it is in apparent tension with the 1995 biochemistry, and the resolution is that both measured an isolated, intrinsically nonselective domain. reaction_mf_exact accepted_mf 1 human ACAA1 genes/human/ACAA1/ACAA1-ai-review.yaml ACAA1 P09110 NCBITaxon:9606 Homo sapiens 15 R-HSA-390250 3-ketohexacosanoyl-CoA + CoASH => tetracosanoyl-CoA + acetyl-CoA yes reactome/R-HSA-390250.md 3-ketohexacosanoyl-CoA + CoASH => tetracosanoyl-CoA + acetyl-CoA Homo sapiens GO:0003988 acetyl-CoA C-acyltransferase activity TAS enables MF false ACCEPT Reactome (TAS) assignment of the 3-ketoacyl-CoA thiolase activity to the specific reaction 3-ketohexacosanoyl-CoA + CoA => tetracosanoyl-CoA + acetyl-CoA, an instance of the core thiolase step. reaction_mf_exact accepted_mf 1 human ACACA genes/human/ACACA/ACACA-ai-review.yaml ACACA Q13085 NCBITaxon:9606 Homo sapiens 22 R-HSA-200555 Formation of Malonyl-CoA from Acetyl-CoA (liver) yes reactome/R-HSA-200555.md Formation of Malonyl-CoA from Acetyl-CoA (liver) Homo sapiens GO:0003989 acetyl-CoA carboxylase activity TAS enables MF false ACCEPT Reactome TAS annotation to the enzyme's core catalytic activity (malonyl-CoA formation in liver). Correct core molecular function. reaction_mf_exact accepted_mf 1 human ACACA genes/human/ACACA/ACACA-ai-review.yaml ACACA Q13085 NCBITaxon:9606 Homo sapiens 23 R-HSA-75851 Btn-ACACA:2Mn2+ polymer carboxylates Ac-CoA to form Mal-CoA yes reactome/R-HSA-75851.md Btn-ACACA:2Mn2+ polymer carboxylates Ac-CoA to form Mal-CoA Homo sapiens GO:0003989 acetyl-CoA carboxylase activity TAS enables MF false ACCEPT Reactome TAS annotation to acetyl-CoA carboxylase activity (biotinylated ACACA polymer carboxylating acetyl-CoA to malonyl-CoA). Correct core molecular function; redundant with the experimental IDA annotations. reaction_mf_exact accepted_mf 1 human ACACB genes/human/ACACB/ACACB-ai-review.yaml ACACB O00763 NCBITaxon:9606 Homo sapiens 37 R-HSA-8876889 Btn-ACACB:2Mn2+ polymer carboxylates Ac-CoA to form Mal-CoA yes reactome/R-HSA-8876889.md Btn-ACACB:2Mn2+ polymer carboxylates Ac-CoA to form Mal-CoA Homo sapiens GO:0003989 acetyl-CoA carboxylase activity TAS enables MF false ACCEPT Reactome TAS to the core acetyl-CoA carboxylase activity, from the reaction in which the biotinylated ACACB polymer carboxylates acetyl-CoA to malonyl-CoA. Correct core function. Accept. reaction_mf_exact accepted_mf 1 human ACER1 genes/human/ACER1/ACER1-ai-review.yaml ACER1 Q8TDN7 NCBITaxon:9606 Homo sapiens 15 R-HSA-428231 ACER1 hydrolyzes ceramide (endoplasmic reticulum) yes reactome/R-HSA-428231.md ACER1 hydrolyzes ceramide (endoplasmic reticulum) Homo sapiens GO:0017040 N-acylsphingosine amidohydrolase activity TAS enables MF false ACCEPT Reactome (traceable author statement) of the core ceramidase activity of ACER1 at the ER membrane. Correct and consistent with experimental evidence. reaction_mf_exact accepted_mf 1 human ACER2 genes/human/ACER2/ACER2-ai-review.yaml ACER2 Q5QJU3 NCBITaxon:9606 Homo sapiens 12 R-HSA-428205 ACER2 hydrolyzes ceramide (Golgi) yes reactome/R-HSA-428205.md ACER2 hydrolyzes ceramide (Golgi) Homo sapiens GO:0017040 N-acylsphingosine amidohydrolase activity TAS enables MF false ACCEPT Reactome curated reaction 'ACER2 hydrolyzes ceramide (Golgi)' asserting the core ceramidase activity. Correct. reaction_mf_exact accepted_mf 1 human ADAM10 genes/human/ADAM10/ADAM10-ai-review.yaml ADAM10 O14672 NCBITaxon:9606 Homo sapiens 48 R-HSA-4224014 E-cadherin degradation by ADAM10, ADAM15 yes reactome/R-HSA-4224014.md E-cadherin degradation by ADAM10, ADAM15 Homo sapiens GO:0004222 metalloendopeptidase activity TAS enables MF false ACCEPT ADAM10 is a zinc-dependent metalloendopeptidase (EC 3.4.24.81) that hydrolyzes peptide bonds in substrate ectodomains via its HEXGHXXGXXHD catalytic motif. reaction_mf_exact accepted_mf 1 human ADPRS genes/human/ADPRS/ADPRS-ai-review.yaml ADPRS Q9NX46 NCBITaxon:9606 Homo sapiens 46 R-HSA-8952903 ADPRHL2 hydrolyses poly(ADP-ribose) yes reactome/R-HSA-8952903.md ADPRHL2 hydrolyses poly(ADP-ribose) Homo sapiens GO:0004649 poly(ADP-ribose) glycohydrolase activity TAS enables MF false ACCEPT Reactome reaction 'ADPRHL2 hydrolyses poly(ADP-ribose)' - a faithful, single-gene statement. reaction_mf_exact accepted_mf 1 human AGO2 genes/human/AGO2/AGO2-ai-review.yaml AGO2 Q9UKV8 NCBITaxon:9606 Homo sapiens 82 R-HSA-426520 Endonucleolytic RISC hydrolyzes target RNAs yes reactome/R-HSA-426520.md Endonucleolytic RISC hydrolyzes target RNAs Homo sapiens GO:0004521 RNA endonuclease activity TAS enables MF false ACCEPT AGO2 supports RNA endonuclease activity in the specified molecular or cellular context. reaction_mf_exact accepted_mf 1 human AGPAT2 genes/human/AGPAT2/AGPAT2-ai-review.yaml AGPAT2 O15120 NCBITaxon:9606 Homo sapiens 14 R-HSA-75885 1-acyl LPA is acylated to PA by AGPAT (LPAAT) yes reactome/R-HSA-75885.md 1-acyl LPA is acylated to PA by AGPAT (LPAAT) Homo sapiens GO:0003841 1-acylglycerol-3-phosphate O-acyltransferase activity TAS enables MF false ACCEPT Reactome assigns AGPAT2 to the LPA-to-PA acylation reaction. reaction_mf_exact accepted_mf multi_gene_reactome_id 3 human AHR genes/human/AHR/AHR-ai-review.yaml AHR P35869 NCBITaxon:9606 Homo sapiens 27 R-HSA-8936849 AHR:2xHSP90:AIP:PTGES3 binds TCDD yes reactome/R-HSA-8936849.md AHR:2xHSP90:AIP:PTGES3 binds TCDD Homo sapiens GO:0004879 nuclear receptor activity TAS enables MF false ACCEPT AHR is a ligand-activated intracellular receptor/transcription factor. Ligand binding, nuclear translocation, ARNT heterodimerization, and target-gene activation are all well supported. reaction_mf_exact accepted_mf 1 human AHR genes/human/AHR/AHR-ai-review.yaml AHR P35869 NCBITaxon:9606 Homo sapiens 28 R-HSA-8937191 AHR:TCDD:2xHSP90AB1:AIP:PTGES3 dissociates yes reactome/R-HSA-8937191.md AHR:TCDD:2xHSP90AB1:AIP:PTGES3 dissociates Homo sapiens GO:0004879 nuclear receptor activity TAS enables MF false ACCEPT AHR is a ligand-activated intracellular receptor/transcription factor. Ligand binding, nuclear translocation, ARNT heterodimerization, and target-gene activation are all well supported. reaction_mf_exact accepted_mf 1 human AKR1C4 genes/human/AKR1C4/AKR1C4-ai-review.yaml AKR1C4 P17516 NCBITaxon:9606 Homo sapiens 32 R-HSA-192036 5Beta-cholesten-7alpha, 12alpha-diol-3-one is reduced to 5beta-cholestan-3alpha, 7alpha, 12alpha-triol yes reactome/R-HSA-192036.md 5Beta-cholesten-7alpha, 12alpha-diol-3-one is reduced to 5beta-cholestan-3alpha, 7alpha, 12alpha-triol Homo sapiens GO:0008106 alcohol dehydrogenase (NADP+) activity TAS enables MF false ACCEPT The broad redox or metabolic annotation accurately describes AKR1C4 catalytic function. reaction_mf_exact accepted_mf 1 human AKR1C4 genes/human/AKR1C4/AKR1C4-ai-review.yaml AKR1C4 P17516 NCBITaxon:9606 Homo sapiens 33 R-HSA-192160 5beta-cholestan-7alpha-ol-3-one is reduced to 5beta-cholestan-3alpha, 7alpha-diol yes reactome/R-HSA-192160.md 5beta-cholestan-7alpha-ol-3-one is reduced to 5beta-cholestan-3alpha, 7alpha-diol Homo sapiens GO:0008106 alcohol dehydrogenase (NADP+) activity TAS enables MF false ACCEPT The broad redox or metabolic annotation accurately describes AKR1C4 catalytic function. reaction_mf_exact accepted_mf 1 human AKR1C4 genes/human/AKR1C4/AKR1C4-ai-review.yaml AKR1C4 P17516 NCBITaxon:9606 Homo sapiens 34 R-HSA-193758 5beta-cholestan-7alpha,24(S)-diol-3-one is reduced to 5beta-cholestan-3alpha,7alpha,24(S)-triol yes reactome/R-HSA-193758.md 5beta-cholestan-7alpha,24(S)-diol-3-one is reduced to 5beta-cholestan-3alpha,7alpha,24(S)-triol Homo sapiens GO:0008106 alcohol dehydrogenase (NADP+) activity TAS enables MF false ACCEPT The broad redox or metabolic annotation accurately describes AKR1C4 catalytic function. reaction_mf_exact accepted_mf 1 human AKR1C4 genes/human/AKR1C4/AKR1C4-ai-review.yaml AKR1C4 P17516 NCBITaxon:9606 Homo sapiens 35 R-HSA-193781 TODO: Fetch title no GO:0008106 alcohol dehydrogenase (NADP+) activity TAS enables MF false ACCEPT The broad redox or metabolic annotation accurately describes AKR1C4 catalytic function. reaction_mf_exact accepted_mf uncached_reactome|missing_reactome_title 1 human AKR1C4 genes/human/AKR1C4/AKR1C4-ai-review.yaml AKR1C4 P17516 NCBITaxon:9606 Homo sapiens 36 R-HSA-193800 5Beta-cholestan-7alpha,12alpha,27-triol-3-one is reduced to 5beta-cholestan-3alpha,7alpha,12alpha,27-tetrol yes reactome/R-HSA-193800.md 5Beta-cholestan-7alpha,12alpha,27-triol-3-one is reduced to 5beta-cholestan-3alpha,7alpha,12alpha,27-tetrol Homo sapiens GO:0008106 alcohol dehydrogenase (NADP+) activity TAS enables MF false ACCEPT The broad redox or metabolic annotation accurately describes AKR1C4 catalytic function. reaction_mf_exact accepted_mf 1 human AKR1C4 genes/human/AKR1C4/AKR1C4-ai-review.yaml AKR1C4 P17516 NCBITaxon:9606 Homo sapiens 37 R-HSA-193841 5beta-cholestan-7alpha,27-diol-3-one is reduced to 5beta-cholestan-3alpha,7alpha,27-triol yes reactome/R-HSA-193841.md 5beta-cholestan-7alpha,27-diol-3-one is reduced to 5beta-cholestan-3alpha,7alpha,27-triol Homo sapiens GO:0008106 alcohol dehydrogenase (NADP+) activity TAS enables MF false ACCEPT The broad redox or metabolic annotation accurately describes AKR1C4 catalytic function. reaction_mf_exact accepted_mf 1 human AKR1C4 genes/human/AKR1C4/AKR1C4-ai-review.yaml AKR1C4 P17516 NCBITaxon:9606 Homo sapiens 38 R-HSA-2855252 AKRs reduce RBP2:atRAL to RBP2:atROL yes reactome/R-HSA-2855252.md AKRs reduce RBP2:atRAL to RBP2:atROL Homo sapiens GO:0008106 alcohol dehydrogenase (NADP+) activity TAS enables MF false ACCEPT The broad redox or metabolic annotation accurately describes AKR1C4 catalytic function. reaction_mf_exact accepted_mf 1 human AKT1 genes/human/AKT1/AKT1-ai-review.yaml AKT1 P31749 NCBITaxon:9606 Homo sapiens 131 R-HSA-1358791 Phosphorylation of USP8 by P-AKT yes reactome/R-HSA-1358791.md Phosphorylation of USP8 by P-AKT Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT AKT phosphorylates USP8 at Thr945 in the cytosol, stabilizing the USP8–NRDP1 regulatory pathway. reaction_mf_exact accepted_mf 1 human AKT1 genes/human/AKT1/AKT1-ai-review.yaml AKT1 P31749 NCBITaxon:9606 Homo sapiens 132 R-HSA-198599 AKT phosphorylates MDM2 yes reactome/R-HSA-198599.md AKT phosphorylates MDM2 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT AKT phosphorylates MDM2 at Ser166 and Ser188 in the cytosol, regulating MDM2 stability and subsequent signaling. reaction_mf_exact accepted_mf 1 human AKT1 genes/human/AKT1/AKT1-ai-review.yaml AKT1 P31749 NCBITaxon:9606 Homo sapiens 133 R-HSA-198609 AKT phosphorylates TSC2, inhibiting it yes reactome/R-HSA-198609.md AKT phosphorylates TSC2, inhibiting it Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT AKT phosphorylates TSC2 at Ser939 and Thr1462 in the cytosol, inhibiting the TSC regulatory branch upstream of mTORC1. reaction_mf_exact accepted_mf 1 human AKT1 genes/human/AKT1/AKT1-ai-review.yaml AKT1 P31749 NCBITaxon:9606 Homo sapiens 134 R-HSA-198611 AKT phosphorylates IKKalpha yes reactome/R-HSA-198611.md AKT phosphorylates IKKalpha Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT AKT phosphorylates the IKK subunit CHUK at Thr23 in the cytosol. reaction_mf_exact accepted_mf 1 human AKT1 genes/human/AKT1/AKT1-ai-review.yaml AKT1 P31749 NCBITaxon:9606 Homo sapiens 135 R-HSA-198613 AKT phosphorylates p21Cip1 and p27Kip1 yes reactome/R-HSA-198613.md AKT phosphorylates p21Cip1 and p27Kip1 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT AKT phosphorylates the cell-cycle inhibitors CDKN1A and CDKN1B in the cytosol. reaction_mf_exact accepted_mf 1 human AKT1 genes/human/AKT1/AKT1-ai-review.yaml AKT1 P31749 NCBITaxon:9606 Homo sapiens 136 R-HSA-198621 AKT phosphorylates caspase-9 yes reactome/R-HSA-198621.md AKT phosphorylates caspase-9 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT AKT phosphorylates caspase-9 at Ser196 in the cytosol, inhibiting this apoptotic protease. reaction_mf_exact accepted_mf 1 human AKT1 genes/human/AKT1/AKT1-ai-review.yaml AKT1 P31749 NCBITaxon:9606 Homo sapiens 137 R-HSA-199298 AKT phosphorylates CREB1 yes reactome/R-HSA-199298.md AKT phosphorylates CREB1 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT AKT phosphorylates CREB1 at Ser133 in the nucleoplasm, linking PI3K signaling to CREB-dependent transcription. reaction_mf_exact accepted_mf 1 human AKT1 genes/human/AKT1/AKT1-ai-review.yaml AKT1 P31749 NCBITaxon:9606 Homo sapiens 138 R-HSA-199299 AKT phosphorylates FOXO transcription factors yes reactome/R-HSA-199299.md AKT phosphorylates FOXO transcription factors Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Nuclear AKT phosphorylates FOXO1, FOXO3 and FOXO4 at defined serine/threonine sites; the event explicitly distinguishes the less certain FOXO6 inference. reaction_mf_exact accepted_mf 1 human AKT1 genes/human/AKT1/AKT1-ai-review.yaml AKT1 P31749 NCBITaxon:9606 Homo sapiens 139 R-HSA-199839 AKT can phosphorylate RSK yes reactome/R-HSA-199839.md AKT can phosphorylate RSK Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT The event models AKT phosphorylation of RPS6KB2 in the nucleoplasm; this is the nuclear S6 kinase substrate, not a transfer of its activity to AKT. reaction_mf_exact accepted_mf 1 human AKT1 genes/human/AKT1/AKT1-ai-review.yaml AKT1 P31749 NCBITaxon:9606 Homo sapiens 140 R-HSA-199863 AKT can phosphorylate NR4A1 (NUR77) yes reactome/R-HSA-199863.md AKT can phosphorylate NR4A1 (NUR77) Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT The event models nuclear AKT phosphorylation of NR4A1 but explicitly notes subsequent work favoring RSK/MSK as the physiological NR4A1 kinase. reaction_mf_exact accepted_mf 1 human AKT1 genes/human/AKT1/AKT1-ai-review.yaml AKT1 P31749 NCBITaxon:9606 Homo sapiens 141 R-HSA-200143 AKT phosphorylates AKT1S1 (PRAS40) yes reactome/R-HSA-200143.md AKT phosphorylates AKT1S1 (PRAS40) Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT AKT phosphorylates AKT1S1/PRAS40 in the cytosol, relieving its inhibition of mTORC1. reaction_mf_exact accepted_mf 1 human AKT1 genes/human/AKT1/AKT1-ai-review.yaml AKT1 P31749 NCBITaxon:9606 Homo sapiens 142 R-HSA-211164 AKT phosphorylates FOXO1A yes reactome/R-HSA-211164.md AKT phosphorylates FOXO1A Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT AKT catalyzes FOXO1 phosphorylation at Thr24, Ser256 and Ser319 in the nucleoplasm. reaction_mf_exact accepted_mf 1 human AKT1 genes/human/AKT1/AKT1-ai-review.yaml AKT1 P31749 NCBITaxon:9606 Homo sapiens 143 R-HSA-2399941 AKT1 E17K mutant phosphorylates BAD yes reactome/R-HSA-2399941.md AKT1 E17K mutant phosphorylates BAD Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT The cancer event models cytosolic AKT1 E17K phosphorylation of BAD, retaining the normal AKT serine/threonine kinase chemistry. reaction_mf_exact accepted_mf 1 human AKT1 genes/human/AKT1/AKT1-ai-review.yaml AKT1 P31749 NCBITaxon:9606 Homo sapiens 144 R-HSA-2399966 AKT1 E17K mutant phosphorylates GSK3 yes reactome/R-HSA-2399966.md AKT1 E17K mutant phosphorylates GSK3 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT AKT1 E17K retains cytosolic phosphorylation of GSK3, an inhibitory modification of that substrate. reaction_mf_exact accepted_mf 1 human AKT1 genes/human/AKT1/AKT1-ai-review.yaml AKT1 P31749 NCBITaxon:9606 Homo sapiens 145 R-HSA-2399969 AKT1 E17K mutant phosphorylates p21Cip1 and p27Kip1 yes reactome/R-HSA-2399969.md AKT1 E17K mutant phosphorylates p21Cip1 and p27Kip1 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT AKT1 E17K phosphorylates CDKN1B; extension to CDKN1A is explicitly predicted rather than experimentally tested in this event. reaction_mf_exact accepted_mf 1 human AKT1 genes/human/AKT1/AKT1-ai-review.yaml AKT1 P31749 NCBITaxon:9606 Homo sapiens 146 R-HSA-2399977 AKT1 E17K mutant phosphorylates AKT1S1 (PRAS40) yes reactome/R-HSA-2399977.md AKT1 E17K mutant phosphorylates AKT1S1 (PRAS40) Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT The event predicts cytosolic AKT1 E17K phosphorylation of PRAS40 from the established wild-type reaction; mutant-specific testing is not claimed. reaction_mf_exact accepted_mf 1 human AKT1 genes/human/AKT1/AKT1-ai-review.yaml AKT1 P31749 NCBITaxon:9606 Homo sapiens 147 R-HSA-2399981 AKT1 E17K mutant phosphorylates MDM2 yes reactome/R-HSA-2399981.md AKT1 E17K mutant phosphorylates MDM2 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT The event predicts cytosolic AKT1 E17K phosphorylation of MDM2 from the established wild-type reaction; mutant-specific testing is not claimed. reaction_mf_exact accepted_mf 1 human AKT1 genes/human/AKT1/AKT1-ai-review.yaml AKT1 P31749 NCBITaxon:9606 Homo sapiens 148 R-HSA-2399982 AKT1 E17K mutant phosphorylates TSC2, inhibiting it yes reactome/R-HSA-2399982.md AKT1 E17K mutant phosphorylates TSC2, inhibiting it Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT The event predicts cytosolic AKT1 E17K phosphorylation and inhibition of TSC2 from the established wild-type reaction. reaction_mf_exact accepted_mf 1 human AKT1 genes/human/AKT1/AKT1-ai-review.yaml AKT1 P31749 NCBITaxon:9606 Homo sapiens 149 R-HSA-2399985 AKT1 E17K mutant phosphorylates caspase-9 yes reactome/R-HSA-2399985.md AKT1 E17K mutant phosphorylates caspase-9 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT The event predicts cytosolic AKT1 E17K phosphorylation of caspase-9 from the established wild-type reaction. reaction_mf_exact accepted_mf 1 human AKT1 genes/human/AKT1/AKT1-ai-review.yaml AKT1 P31749 NCBITaxon:9606 Homo sapiens 150 R-HSA-2399988 AKT1 E17K mutant phosphorylates NR4A1 (NUR77) yes reactome/R-HSA-2399988.md AKT1 E17K mutant phosphorylates NR4A1 (NUR77) Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT The event predicts nuclear AKT1 E17K phosphorylation of NR4A1; its wild-type counterpart also records uncertainty about the physiological NR4A1 kinase. reaction_mf_exact accepted_mf 1 human AKT1 genes/human/AKT1/AKT1-ai-review.yaml AKT1 P31749 NCBITaxon:9606 Homo sapiens 151 R-HSA-2399992 AKT1 E17K mutant phosphorylates forkhead box transcription factors yes reactome/R-HSA-2399992.md AKT1 E17K mutant phosphorylates forkhead box transcription factors Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT AKT1 E17K phosphorylates FOXO3; the model extends this to other FOXO proteins with explicit experimental limitations. reaction_mf_exact accepted_mf 1 human AKT1 genes/human/AKT1/AKT1-ai-review.yaml AKT1 P31749 NCBITaxon:9606 Homo sapiens 152 R-HSA-2399996 AKT1 E17K mutant phosphorylates CREB1 yes reactome/R-HSA-2399996.md AKT1 E17K mutant phosphorylates CREB1 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT The event predicts nuclear AKT1 E17K phosphorylation of CREB1 from the established wild-type reaction. reaction_mf_exact accepted_mf 1 human AKT1 genes/human/AKT1/AKT1-ai-review.yaml AKT1 P31749 NCBITaxon:9606 Homo sapiens 153 R-HSA-2399999 AKT1 E17K mutant phosphorylates RSK yes reactome/R-HSA-2399999.md AKT1 E17K mutant phosphorylates RSK Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT The event predicts nuclear AKT1 E17K phosphorylation of RPS6KB2 from the wild-type reaction. reaction_mf_exact accepted_mf 1 human AKT1 genes/human/AKT1/AKT1-ai-review.yaml AKT1 P31749 NCBITaxon:9606 Homo sapiens 154 R-HSA-2400001 AKT1 E17K mutant phosphorylates CHUK (IKKalpha) yes reactome/R-HSA-2400001.md AKT1 E17K mutant phosphorylates CHUK (IKKalpha) Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT The event predicts cytosolic AKT1 E17K phosphorylation of CHUK from the wild-type reaction. reaction_mf_exact accepted_mf 1 human AKT1 genes/human/AKT1/AKT1-ai-review.yaml AKT1 P31749 NCBITaxon:9606 Homo sapiens 155 R-HSA-3769394 AKT phosphorylates CBY1 yes reactome/R-HSA-3769394.md AKT phosphorylates CBY1 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT AKT1/AKT2 phosphorylates CBY1 at Ser20 in a nuclear beta-catenin complex, enabling subsequent 14-3-3-dependent export. reaction_mf_exact accepted_mf 1 human AKT1 genes/human/AKT1/AKT1-ai-review.yaml AKT1 P31749 NCBITaxon:9606 Homo sapiens 156 R-HSA-377186 Activated AKT phosphorylates AKT1S1 (PRAS40) yes reactome/R-HSA-377186.md Activated Akt1 phosphorylates AKT1S1 (PRAS40) Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT AKT phosphorylates AKT1S1/PRAS40 at Thr246; the current model explicitly places the AKT catalyst in the cytosol and the mTORC1 substrate complex at the lysosomal membrane. reaction_mf_exact accepted_mf 1 human AKT1 genes/human/AKT1/AKT1-ai-review.yaml AKT1 P31749 NCBITaxon:9606 Homo sapiens 157 R-HSA-389756 AKT interacts and phosphorylates Cot yes reactome/R-HSA-389756.md AKT interacts and phosphorylates Cot Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT AKT interacts with MAP3K8/Cot and phosphorylates Ser400 in cytosolic assays; Reactome explicitly limits the evidence to in-vitro and overexpression conditions. reaction_mf_exact accepted_mf 1 human AKT1 genes/human/AKT1/AKT1-ai-review.yaml AKT1 P31749 NCBITaxon:9606 Homo sapiens 158 R-HSA-432110 Integrin alpha IIb beta3 T779 phosphorylation blocks SHC binding yes reactome/R-HSA-432110.md Integrin alpha IIb beta3 T779 phosphorylation blocks SHC binding Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT AKT1 can phosphorylate the integrin beta3 cytoplasmic tail at Thr779; the modeled kinase entity is at the plasma membrane. reaction_mf_exact accepted_mf 1 human AKT1 genes/human/AKT1/AKT1-ai-review.yaml AKT1 P31749 NCBITaxon:9606 Homo sapiens 159 R-HSA-6805640 AKT phosphorylates KAT6A yes reactome/R-HSA-6805640.md AKT phosphorylates KAT6A Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT AKT phosphorylates nuclear KAT6A at Thr369, preventing association with PML and reducing KAT6A-mediated TP53 acetylation. reaction_mf_exact accepted_mf 1 human AKT1 genes/human/AKT1/AKT1-ai-review.yaml AKT1 P31749 NCBITaxon:9606 Homo sapiens 160 R-HSA-6805785 AKT phosphorylates PHF20 yes reactome/R-HSA-6805785.md AKT phosphorylates PHF20 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT AKT phosphorylates PHF20 at Ser291 in the nucleoplasm, regulating damage-induced TP53 stabilization. reaction_mf_exact accepted_mf 1 human AKT1 genes/human/AKT1/AKT1-ai-review.yaml AKT1 P31749 NCBITaxon:9606 Homo sapiens 161 R-HSA-8933446 Active AKT phosphorylates DENND1A and DENND1B in response to insulin signaling yes reactome/R-HSA-8933446.md Active AKT phosphorylates DENND1A and DENND1B in response to insulin signaling Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT AKT phosphorylates DENND1A/DENND1B in the cytosol, relieving their autoinhibition and promoting RAB35 activation. reaction_mf_exact accepted_mf 1 human AKT1 genes/human/AKT1/AKT1-ai-review.yaml AKT1 P31749 NCBITaxon:9606 Homo sapiens 162 R-HSA-8948757 AKT phosphorylates MKRN1 yes reactome/R-HSA-8948757.md AKT phosphorylates MKRN1 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT AKT1 phosphorylates cytosolic MKRN1 at Ser109, stabilizing this ubiquitin ligase in an EGF-responsive regulatory circuit. reaction_mf_exact accepted_mf 1 human AKT1 genes/human/AKT1/AKT1-ai-review.yaml AKT1 P31749 NCBITaxon:9606 Homo sapiens 163 R-HSA-9624526 AKT phosphorylates FOXO3 downstream of ESR1 and EGFR yes reactome/R-HSA-9624526.md AKT phosphorylates FOXO3 downstream of ESR1 and EGFR Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT AKT phosphorylates FOXO3 in the nucleoplasm downstream of estrogen/EGFR signaling, promoting FOXO3 nuclear export. reaction_mf_exact accepted_mf 1 human AKT1 genes/human/AKT1/AKT1-ai-review.yaml AKT1 P31749 NCBITaxon:9606 Homo sapiens 164 R-HSA-9699579 AKT phosphorylates FOXO3 downstream of FLT3 yes reactome/R-HSA-9699579.md AKT phosphorylates FOXO3 downstream of FLT3 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT AKT phosphorylates FOXO3 in the nucleoplasm downstream of FLT3 signaling, promoting FOXO3 nuclear export. reaction_mf_exact accepted_mf 1 human AKT1 genes/human/AKT1/AKT1-ai-review.yaml AKT1 P31749 NCBITaxon:9606 Homo sapiens 165 R-NUL-3139045 AKT phosphorylates Bad yes reactome/R-NUL-3139045.md AKT phosphorylates Bad Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT This mixed-species reaction uses recombinant human AKT to phosphorylate recombinant mouse Bad at Ser136; the catalyst is human and the modeled location is cytosol. reaction_mf_exact accepted_mf 1 human AKT1 genes/human/AKT1/AKT1-ai-review.yaml AKT1 P31749 NCBITaxon:9606 Homo sapiens 166 R-NUL-8939977 Activated AKT1 phosphorylates Runx2 yes reactome/R-NUL-8939977.md Activated AKT1 phosphorylates Runx2 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT This mixed-species reaction uses recombinant human AKT1 to phosphorylate mouse Runx2-5 at Ser203, Thr205 and Thr207; the model places the catalyst in nucleoplasm. reaction_mf_exact accepted_mf 1 human AKT1 genes/human/AKT1/AKT1-ai-review.yaml AKT1 P31749 NCBITaxon:9606 Homo sapiens 199 R-HSA-9860792 Phospho-AKT1 phosphorylates NOS3 (eNOS) on serine-1177 yes reactome/R-HSA-9860792.md Phospho-AKT1 phosphorylates NOS3 (eNOS) on serine-1177 Homo sapiens GO:0106310 protein serine kinase activity TAS enables MF false ACCEPT AKT1 phosphorylates NOS3 Ser1177 at the plasma membrane during endothelial flow signaling. reaction_mf_exact accepted_mf 1 human AKT1 genes/human/AKT1/AKT1-ai-review.yaml AKT1 P31749 NCBITaxon:9606 Homo sapiens 204 R-HSA-9841265 p-S473-AKT1 phosphorylates ESR1 on serine-167 yes reactome/R-HSA-9841265.md p-S473-AKT1 phosphorylates ESR1 on serine-167 Homo sapiens GO:0106310 protein serine kinase activity TAS enables MF false ACCEPT Activated cytosolic AKT1 phosphorylates ESR1 at Ser167 in a mitochondrial stress-response signaling branch. reaction_mf_exact accepted_mf 1 human ALDH6A1 genes/human/ALDH6A1/ALDH6A1-ai-review.yaml ALDH6A1 Q02252 NCBITaxon:9606 Homo sapiens 9 R-HSA-70893 methylmalonate semialdehyde + NAD+ + CoA + H2O => propionyl-CoA + HCO3- + NADH + H+ yes reactome/R-HSA-70893.md methylmalonate semialdehyde + NAD+ + CoA + H2O => propionyl-CoA + HCO3- + NADH + H+ Homo sapiens GO:0004491 methylmalonate-semialdehyde dehydrogenase (acylating, NAD) activity TAS enables MF false ACCEPT Core catalytic activity, author-asserted by Reactome for the reaction methylmalonate semialdehyde + NAD+ + CoA + H2O => propionyl-CoA + HCO3- + NADH + H+. Consistent with the experimental IMP annotation. reaction_mf_exact accepted_mf 1 human ALG1 genes/human/ALG1/ALG1-ai-review.yaml ALG1 Q9BT22 NCBITaxon:9606 Homo sapiens 9 R-HSA-446218 Addition of the first mannose to the N-glycan precursor by ALG1 yes reactome/R-HSA-446218.md Addition of the first mannose to the N-glycan precursor by ALG1 Homo sapiens GO:0004578 chitobiosyldiphosphodolichol beta-mannosyltransferase activity TAS enables MF false ACCEPT The normal Reactome ALG1 reaction matches its specific catalytic function. reaction_mf_exact accepted_mf local_module_mf 1 n_glycan_llo_assembly_cytoplasmic:GO:0004578 chitobiosyldiphosphodolichol beta-mannosyltransferase activity human ALG1 genes/human/ALG1/ALG1-ai-review.yaml ALG1 Q9BT22 NCBITaxon:9606 Homo sapiens 10 R-HSA-4549382 Defective ALG1 does not transfer the first Man to the N-glycan precursor yes reactome/R-HSA-4549382.md Defective ALG1 does not transfer the first Man to the N-glycan precursor Homo sapiens GO:0004578 chitobiosyldiphosphodolichol beta-mannosyltransferase activity TAS enables MF false ACCEPT The disease reaction describes loss of the established wild-type ALG1 activity. reaction_mf_exact accepted_mf reactome_disease_event 1 human ALG11 genes/human/ALG11/ALG11-ai-review.yaml ALG11 Q2TAA5 NCBITaxon:9606 Homo sapiens 12 R-HSA-4551297 Defective ALG11 does not transfer Man to the N-glycan precursor yes reactome/R-HSA-4551297.md Defective ALG11 does not transfer Man to the N-glycan precursor Homo sapiens GO:0000026 alpha-1,2-mannosyltransferase activity TAS enables MF false ACCEPT Reactome author-statement annotation to the general alpha-1,2-mannosyltransferase activity, the parent of the specific ALG11 activity term. reaction_mf_exact accepted_mf reactome_disease_event 1 human ALG2 genes/human/ALG2/ALG2-ai-review.yaml ALG2 Q9H553 NCBITaxon:9606 Homo sapiens 22 R-HSA-446208 Addition of a second mannose to the N-glycan precursor by ALG2 yes reactome/R-HSA-446208.md Addition of a second mannose to the N-glycan precursor by ALG2 Homo sapiens GO:0000033 alpha-1,3-mannosyltransferase activity TAS enables MF false ACCEPT Reactome-asserted alpha-1,3-mannosyltransferase activity (addition of a second mannose to the N-glycan precursor by ALG2). Correct parent-level molecular function. reaction_mf_exact accepted_mf local_module_mf 1 n_glycan_llo_assembly_cytoplasmic:GO:0004378 GDP-Man:Man(1)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase activity human ALG2 genes/human/ALG2/ALG2-ai-review.yaml ALG2 Q9H553 NCBITaxon:9606 Homo sapiens 23 R-HSA-4549368 Defective ALG2 does not transfer a second Man to N-glycan precursor yes reactome/R-HSA-4549368.md Defective ALG2 does not transfer a second Man to N-glycan precursor Homo sapiens GO:0000033 alpha-1,3-mannosyltransferase activity TAS enables MF false ACCEPT Reactome-asserted alpha-1,3-mannosyltransferase activity (from the defective-ALG2 / CDG-1i pathway). Correct parent-level molecular function. reaction_mf_exact accepted_mf reactome_disease_event 1 human ALG5 genes/human/ALG5/ALG5-ai-review.yaml ALG5 Q9Y673 NCBITaxon:9606 Homo sapiens 11 R-HSA-446214 Synthesis of dolichyl-phosphate-glucose yes reactome/R-HSA-446214.md Synthesis of dolichyl-phosphate-glucose Homo sapiens GO:0004581 dolichyl-phosphate beta-glucosyltransferase activity TAS enables MF false ACCEPT Reactome-asserted dolichyl-phosphate beta-glucosyltransferase activity. Reactome describes ALG5 catalyzing UDP-glucose + dolichyl phosphate to form Dol-P-Glc on the cytosolic face of the ER membrane. reaction_mf_exact accepted_mf 1 human ALK genes/human/ALK/ALK-ai-review.yaml ALK Q9UM73 NCBITaxon:9606 Homo sapiens 7 R-HSA-9700168 Active ALK phosphorylates IRS1 yes reactome/R-HSA-9700168.md Active ALK phosphorylates IRS1 Homo sapiens GO:0004713 protein tyrosine kinase activity TAS enables MF false ACCEPT Retain the tyrosine kinase activity at the resolution of this reaction model. reaction_mf_exact accepted_mf 1 human ALK genes/human/ALK/ALK-ai-review.yaml ALK Q9UM73 NCBITaxon:9606 Homo sapiens 8 R-HSA-9700171 Active ALK phosphorylates PLCG1 yes reactome/R-HSA-9700171.md Active ALK phosphorylates PLCG1 Homo sapiens GO:0004713 protein tyrosine kinase activity TAS enables MF false ACCEPT Retain the tyrosine kinase activity at the resolution of this reaction model. reaction_mf_exact accepted_mf 1 human ALK genes/human/ALK/ALK-ai-review.yaml ALK Q9UM73 NCBITaxon:9606 Homo sapiens 9 R-HSA-9700175 Active ALK phosphorylates SHC1 yes reactome/R-HSA-9700175.md Active ALK phosphorylates SHC1 Homo sapiens GO:0004713 protein tyrosine kinase activity TAS enables MF false ACCEPT Retain the tyrosine kinase activity at the resolution of this reaction model. reaction_mf_exact accepted_mf 1 human ALK genes/human/ALK/ALK-ai-review.yaml ALK Q9UM73 NCBITaxon:9606 Homo sapiens 10 R-HSA-9700193 ALK mutants phosphorylate SHC1 yes reactome/R-HSA-9700193.md ALK mutants phosphorylate SHC1 Homo sapiens GO:0004713 protein tyrosine kinase activity TAS enables MF false ACCEPT Retain the tyrosine kinase activity at the resolution of this reaction model. reaction_mf_exact accepted_mf 1 human ALK genes/human/ALK/ALK-ai-review.yaml ALK Q9UM73 NCBITaxon:9606 Homo sapiens 11 R-HSA-9701488 Active ALK phosphorylates JAK3 yes reactome/R-HSA-9701488.md Active ALK phosphorylates JAK3 Homo sapiens GO:0004713 protein tyrosine kinase activity TAS enables MF false ACCEPT Retain the tyrosine kinase activity at the resolution of this reaction model. reaction_mf_exact accepted_mf 1 human ALK genes/human/ALK/ALK-ai-review.yaml ALK Q9UM73 NCBITaxon:9606 Homo sapiens 12 R-NUL-9700161 p-7Y ALK dimer phosphorylates Frs2 yes reactome/R-NUL-9700161.md p-7Y ALK dimer phosphorylates Frs2 Homo sapiens GO:0004713 protein tyrosine kinase activity TAS enables MF false ACCEPT Retain the tyrosine kinase activity at the resolution of this reaction model. reaction_mf_exact accepted_mf 1 human ALK genes/human/ALK/ALK-ai-review.yaml ALK Q9UM73 NCBITaxon:9606 Homo sapiens 13 R-NUL-9700655 Active ALK phosphorylates Src yes reactome/R-NUL-9700655.md Active ALK phosphorylates Src Homo sapiens GO:0004713 protein tyrosine kinase activity TAS enables MF false ACCEPT Retain the tyrosine kinase activity at the resolution of this reaction model. reaction_mf_exact accepted_mf 1 human AMACR genes/human/AMACR/AMACR-ai-review.yaml AMACR Q9UHK6 NCBITaxon:9606 Homo sapiens 14 R-HSA-192056 Isomerization of 25(R) THCA-CoA to 25(S) THCA-CoA yes reactome/R-HSA-192056.md Isomerization of 25(R) THCA-CoA to 25(S) THCA-CoA Homo sapiens GO:0008111 alpha-methylacyl-CoA racemase activity TAS enables MF false ACCEPT Reactome (isomerization of 25(R) THCA-CoA to 25(S) THCA-CoA) asserts the racemase activity on a specific bile-acid intermediate substrate. reaction_mf_exact accepted_mf local_module_mf 1 bile_acid_synthesis_sidechain_conjugation:GO:0008111 alpha-methylacyl-CoA racemase activity human AMACR genes/human/AMACR/AMACR-ai-review.yaml AMACR Q9UHK6 NCBITaxon:9606 Homo sapiens 15 R-HSA-193452 Isomerization of 25(R) DHCA-CoA to 25(S) DHCA-CoA yes reactome/R-HSA-193452.md Isomerization of 25(R) DHCA-CoA to 25(S) DHCA-CoA Homo sapiens GO:0008111 alpha-methylacyl-CoA racemase activity TAS enables MF false ACCEPT Reactome (isomerization of 25(R) DHCA-CoA to 25(S) DHCA-CoA) asserts the racemase activity on the DHCA-CoA bile-acid intermediate. reaction_mf_exact accepted_mf 1 human AMACR genes/human/AMACR/AMACR-ai-review.yaml AMACR Q9UHK6 NCBITaxon:9606 Homo sapiens 16 R-HSA-193736 Isomerization of 3,7,24THCA-CoA to (24R, 25R) 3alpha,7alpha,24-trihydroxy-5beta-cholestanoyl-CoA yes reactome/R-HSA-193736.md Isomerization of 3,7,24THCA-CoA to (24R, 25R) 3alpha,7alpha,24-trihydroxy-5beta-cholestanoyl-CoA Homo sapiens GO:0008111 alpha-methylacyl-CoA racemase activity TAS enables MF false ACCEPT Reactome (isomerization of 3,7,24-THCA-CoA) asserts racemase activity on a bile-acid intermediate. Duplicate assertion of the core function. reaction_mf_exact accepted_mf 1 human AMACR genes/human/AMACR/AMACR-ai-review.yaml AMACR Q9UHK6 NCBITaxon:9606 Homo sapiens 17 R-HSA-193763 TODO: Fetch title no GO:0008111 alpha-methylacyl-CoA racemase activity TAS enables MF false ACCEPT Reactome assertion of AMACR racemase activity within the bile-acid synthesis pathway. Duplicate assertion of the core function. reaction_mf_exact accepted_mf uncached_reactome|missing_reactome_title 1 human AMD1 genes/human/AMD1/AMD1-ai-review.yaml AMD1 P17707 NCBITaxon:9606 Homo sapiens 13 R-HSA-351222 S-Adenosyl methionine <=> Decarboxylated-Adenosyl methionine + CO2 yes reactome/R-HSA-351222.md S-Adenosyl methionine <=> Decarboxylated-Adenosyl methionine + CO2 Homo sapiens GO:0004014 adenosylmethionine decarboxylase activity TAS enables MF false ACCEPT Reactome traceable assignment of the core catalytic activity (the reaction S-adenosylmethionine <=> decarboxylated-adenosylmethionine + CO2). Correct core molecular function. reaction_mf_exact accepted_mf 1 human ANG genes/human/ANG/ANG-ai-review.yaml ANG P03950 NCBITaxon:9606 Homo sapiens 22 R-HSA-9708327 ANG cleaves tRNA to yield tRNA halves no GO:0004521 RNA endonuclease activity TAS enables MF false ACCEPT Correct. ANG is an RNase A-family RNA endoribonuclease that catalyzes RNA backbone cleavage. reaction_mf_exact accepted_mf uncached_reactome 1 human APEX1 genes/human/APEX1/APEX1-ai-review.yaml APEX1 P27695 NCBITaxon:9606 Homo sapiens 79 R-HSA-110359 APEX1 mediates endonucleolytic cleavage at the 5' side of the AP site yes reactome/R-HSA-110359.md APEX1 mediates endonucleolytic cleavage at the 5' side of the AP site Homo sapiens GO:0004520 DNA endonuclease activity TAS enables MF false ACCEPT APEX1 has DNA endonuclease activity, cleaving at AP sites. reaction_mf_exact accepted_mf 1 human APOE genes/human/APOE/APOE-ai-review.yaml APOE P02649 NCBITaxon:9606 Homo sapiens 68 R-HSA-2423785 CR:atREs binds apoE and HSPG yes reactome/R-HSA-2423785.md CR:atREs binds apoE and HSPG Homo sapiens GO:0050750 low-density lipoprotein particle receptor binding TAS enables MF false ACCEPT APOE low-density lipoprotein particle receptor binding supports receptor- or proteoglycan-dependent uptake of APOE-containing lipoprotein particles. reaction_mf_exact accepted_mf 1 human ARF1 genes/human/ARF1/ARF1-ai-review.yaml ARF1 P84077 NCBITaxon:9606 Homo sapiens 36 R-HSA-8847883 CYTH proteins stimulate ARF1 GTPase activity yes reactome/R-HSA-8847883.md CYTH proteins stimulate ARF1 GTPase activity Homo sapiens GO:0003924 GTPase activity TAS enables MF false ACCEPT ARF1 is a canonical small GTPase that binds guanine nucleotide and hydrolyzes GTP as part of its membrane-trafficking cycle. reaction_mf_exact accepted_mf 1 human ARHGAP21 genes/human/ARHGAP21/ARHGAP21-ai-review.yaml ARHGAP21 Q5T5U3 NCBITaxon:9606 Homo sapiens 4 R-HSA-8981637 RHOA GAPs stimulate RHOA GTPase activity yes reactome/R-HSA-8981637.md RHOA GAPs stimulate RHOA GTPase activity Homo sapiens GO:0005096 GTPase activator activity TAS enables MF false ACCEPT GAP activity, asserted by Reactome from the reaction 'RHOA GAPs stimulate RHOA GTPase activity'. reaction_mf_exact accepted_mf multi_gene_reactome_id 5 human ARHGAP23 genes/human/ARHGAP23/ARHGAP23-ai-review.yaml ARHGAP23 Q9P227 NCBITaxon:9606 Homo sapiens 2 R-HSA-8981637 RHOA GAPs stimulate RHOA GTPase activity yes reactome/R-HSA-8981637.md RHOA GAPs stimulate RHOA GTPase activity Homo sapiens GO:0005096 GTPase activator activity TAS enables MF false ACCEPT "A set-level Reactome projection that happens to be correct here. The reaction's catalyst is the defined entity ""GTPase activator activity of RHOA GAPs"", so every member receives this term; ARHGAP23's membership is sourced in Reactome's own summary to the FRET specificity screen, which scores it significantly active on RhoA." reaction_mf_exact accepted_mf multi_gene_reactome_id 5 human ARHGAP23 genes/human/ARHGAP23/ARHGAP23-ai-review.yaml ARHGAP23 Q9P227 NCBITaxon:9606 Homo sapiens 3 R-HSA-9013144 RAC1 GAPs stimulate RAC1 GTPase activity yes reactome/R-HSA-9013144.md RAC1 GAPs stimulate RAC1 GTPase activity Homo sapiens GO:0005096 GTPase activator activity TAS enables MF false ACCEPT The RAC1 counterpart of the previous row, from the equivalent RAC1 GAP catalyst set. Reactome sources ARHGAP23's membership to the Rac FRET biosensor study PMID:28114311 and to the family-wide screen, both of which report Rac1-directed activity. reaction_mf_exact accepted_mf multi_gene_reactome_id 2 human ARHGAP29 genes/human/ARHGAP29/ARHGAP29-ai-review.yaml ARHGAP29 Q52LW3 NCBITaxon:9606 Homo sapiens 3 R-HSA-8981637 RHOA GAPs stimulate RHOA GTPase activity yes reactome/R-HSA-8981637.md RHOA GAPs stimulate RHOA GTPase activity Homo sapiens GO:0005096 GTPase activator activity TAS enables MF false ACCEPT ARHGAP29 contributes GAP activity to the RHOA inactivation reaction. reaction_mf_exact accepted_mf multi_gene_reactome_id 5 human ARHGAP4 genes/human/ARHGAP4/ARHGAP4-ai-review.yaml ARHGAP4 P98171 NCBITaxon:9606 Homo sapiens 4 R-HSA-9013144 RAC1 GAPs stimulate RAC1 GTPase activity yes reactome/R-HSA-9013144.md RAC1 GAPs stimulate RAC1 GTPase activity Homo sapiens GO:0005096 GTPase activator activity TAS enables MF false ACCEPT "Reactome places ARHGAP4 in the reaction ""RAC1 GAPs stimulate RAC1 GTPase activity"", which is a substrate-specific claim that the GO term itself cannot carry." reaction_mf_exact accepted_mf multi_gene_reactome_id 2 human ARHGAP6 genes/human/ARHGAP6/ARHGAP6-ai-review.yaml ARHGAP6 O43182 NCBITaxon:9606 Homo sapiens 3 R-HSA-8981637 RHOA GAPs stimulate RHOA GTPase activity yes reactome/R-HSA-8981637.md RHOA GAPs stimulate RHOA GTPase activity Homo sapiens GO:0005096 GTPase activator activity TAS enables MF false ACCEPT Reactome's RHOA-GAP event, and the better-grounded of its two GAP assertions for this protein. reaction_mf_exact accepted_mf multi_gene_reactome_id 5 human ARHGAP6 genes/human/ARHGAP6/ARHGAP6-ai-review.yaml ARHGAP6 O43182 NCBITaxon:9606 Homo sapiens 4 R-HSA-9018806 RAC3 GAPs stimulate RAC3 GTPase activity yes reactome/R-HSA-9018806.md RAC3 GAPs stimulate RAC3 GTPase activity Homo sapiens GO:0005096 GTPase activator activity TAS enables MF false ACCEPT Same term, but reached through a RAC3-GAP claim that rests on a co-immunoprecipitation. reaction_mf_exact accepted_mf 1 human ARHGEF15 genes/human/ARHGEF15/ARHGEF15-ai-review.yaml ARHGEF15 O94989 NCBITaxon:9606 Homo sapiens 6 R-HSA-8980691 RHOA GEFs activate RHOA yes reactome/R-HSA-8980691.md RHOA GEFs activate RHOA Homo sapiens GO:0005085 guanyl-nucleotide exchange factor activity TAS enables MF false ACCEPT Reactome places ARHGEF15 in its confirmed list of RHOA GEFs. Consistent with every other line of evidence, and the citation behind it is a family-wide specificity screen. reaction_mf_exact accepted_mf multi_gene_reactome_id 2 human ARHGEF15 genes/human/ARHGEF15/ARHGEF15-ai-review.yaml ARHGEF15 O94989 NCBITaxon:9606 Homo sapiens 7 R-HSA-9013159 CDC42 GEFs activate CDC42 yes reactome/R-HSA-9013159.md CDC42 GEFs activate CDC42 Homo sapiens GO:0005085 guanyl-nucleotide exchange factor activity TAS enables MF false ACCEPT Reactome places ARHGEF15 in its confirmed list of CDC42 GEFs as well. This is the only point in the entire GO record where CDC42 is named for this gene, and it is supported by three independent measurements. reaction_mf_exact accepted_mf multi_gene_reactome_id 2 human ARHGEF19 genes/human/ARHGEF19/ARHGEF19-ai-review.yaml ARHGEF19 Q8IW93 NCBITaxon:9606 Homo sapiens 3 R-HSA-8980691 RHOA GEFs activate RHOA yes reactome/R-HSA-8980691.md RHOA GEFs activate RHOA Homo sapiens GO:0005085 guanyl-nucleotide exchange factor activity TAS enables MF false ACCEPT Correct. Reactome places ARHGEF19 in its RHOA GEF set on the strength of the 2004 characterisation paper, which is the same paper GO itself never annotated. reaction_mf_exact accepted_mf multi_gene_reactome_id 2 human ARHGEF9 genes/human/ARHGEF9/ARHGEF9-ai-review.yaml ARHGEF9 O43307 NCBITaxon:9606 Homo sapiens 3 R-HSA-9013159 CDC42 GEFs activate CDC42 yes reactome/R-HSA-9013159.md CDC42 GEFs activate CDC42 Homo sapiens GO:0005085 guanyl-nucleotide exchange factor activity TAS enables MF false ACCEPT The CDC42 GEF event explicitly identifies ARHGEF9. reaction_mf_exact accepted_mf multi_gene_reactome_id 2 human ARNT genes/human/ARNT/ARNT-ai-review.yaml ARNT P27540 NCBITaxon:9606 Homo sapiens 42 R-HSA-8937177 AHR:TCDD binds ARNT yes reactome/R-HSA-8937177.md AHR:TCDD binds ARNT Homo sapiens GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific TAS enables MF false ACCEPT DNA-binding transcription activator activity, RNA polymerase II-specific captures the transcription-factor activity of ARNT-containing RNA polymerase II regulatory complexes. reaction_mf_exact accepted_mf 1 human ASCC3 genes/human/ASCC3/ASCC3-ai-review.yaml ASCC3 Q8N3C0 NCBITaxon:9606 Homo sapiens 22 R-HSA-9948300 RQT complex:K63polyUb-80S ribosome dissociates yielding K63polyUb-40S subunit and 60S subunit:peptidyl-tRNA with nascent peptide yes reactome/R-HSA-9948300.md RQT complex:K63polyUb-80S ribosome dissociates yielding K63polyUb-40S subunit and 60S subunit:peptidyl-tRNA with nascent peptide Homo sapiens GO:0016887 ATP hydrolysis activity TAS enables MF false ACCEPT ATP hydrolysis activity is directly tied to ASCC3 helicase-dependent DNA repair and hRQT ribosome splitting. reaction_mf_exact accepted_mf 1 human ATM genes/human/ATM/ATM-ai-review.yaml ATM Q13315 NCBITaxon:9606 Homo sapiens 37 R-HSA-3371567 DBC1 is phosphorylated by ATM/ART yes reactome/R-HSA-3371567.md DBC1 is phosphorylated by ATM/ART Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Retain supported protein serine/threonine kinase activity. reaction_mf_exact accepted_mf 1 human ATM genes/human/ATM/ATM-ai-review.yaml ATM Q13315 NCBITaxon:9606 Homo sapiens 38 R-HSA-349444 Phosphorylation of COP1 at Ser-387 by ATM yes reactome/R-HSA-349444.md Phosphorylation of COP1 at Ser-387 by ATM Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Retain supported protein serine/threonine kinase activity. reaction_mf_exact accepted_mf 1 human ATM genes/human/ATM/ATM-ai-review.yaml ATM Q13315 NCBITaxon:9606 Homo sapiens 39 R-HSA-349455 Phosphorylation of MDM4 by ATM yes reactome/R-HSA-349455.md Phosphorylation of MDM4 by ATM Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Retain supported protein serine/threonine kinase activity. reaction_mf_exact accepted_mf 1 human ATM genes/human/ATM/ATM-ai-review.yaml ATM Q13315 NCBITaxon:9606 Homo sapiens 40 R-HSA-5682598 ATM phosphorylates HERC2 yes reactome/R-HSA-5682598.md ATM phosphorylates HERC2 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Retain supported protein serine/threonine kinase activity. reaction_mf_exact accepted_mf 1 human ATM genes/human/ATM/ATM-ai-review.yaml ATM Q13315 NCBITaxon:9606 Homo sapiens 41 R-HSA-5682983 ATM phosphorylates WHSC1 yes reactome/R-HSA-5682983.md ATM phosphorylates WHSC1 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Retain supported protein serine/threonine kinase activity. reaction_mf_exact accepted_mf 1 human ATM genes/human/ATM/ATM-ai-review.yaml ATM Q13315 NCBITaxon:9606 Homo sapiens 42 R-HSA-5683425 ATM phosphorylates TP53BP1 at DNA DSBs yes reactome/R-HSA-5683425.md ATM phosphorylates TP53BP1 at DNA DSBs Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Retain supported protein serine/threonine kinase activity. reaction_mf_exact accepted_mf 1 human ATM genes/human/ATM/ATM-ai-review.yaml ATM Q13315 NCBITaxon:9606 Homo sapiens 43 R-HSA-5683964 ATM phosphorylates EYA1-4 yes reactome/R-HSA-5683964.md ATM phosphorylates EYA1-4 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Retain supported protein serine/threonine kinase activity. reaction_mf_exact accepted_mf 1 human ATM genes/human/ATM/ATM-ai-review.yaml ATM Q13315 NCBITaxon:9606 Homo sapiens 44 R-HSA-5684140 ATM phosphorylates RBBP8 yes reactome/R-HSA-5684140.md ATM phosphorylates RBBP8 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Retain supported protein serine/threonine kinase activity. reaction_mf_exact accepted_mf 1 human ATM genes/human/ATM/ATM-ai-review.yaml ATM Q13315 NCBITaxon:9606 Homo sapiens 45 R-HSA-5686578 Activated ATM phosphorylates ABL1 yes reactome/R-HSA-5686578.md Activated ATM phosphorylates ABL1 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Retain supported protein serine/threonine kinase activity. reaction_mf_exact accepted_mf 1 human ATM genes/human/ATM/ATM-ai-review.yaml ATM Q13315 NCBITaxon:9606 Homo sapiens 46 R-HSA-5686704 Activated ATM phosphorylates DCLRE1C yes reactome/R-HSA-5686704.md Activated ATM phosphorylates DCLRE1C Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Retain supported protein serine/threonine kinase activity. reaction_mf_exact accepted_mf 1 human ATM genes/human/ATM/ATM-ai-review.yaml ATM Q13315 NCBITaxon:9606 Homo sapiens 47 R-HSA-5693536 ATM phosphorylates MDC1 yes reactome/R-HSA-5693536.md ATM phosphorylates MDC1 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Retain supported protein serine/threonine kinase activity. reaction_mf_exact accepted_mf 1 human ATM genes/human/ATM/ATM-ai-review.yaml ATM Q13315 NCBITaxon:9606 Homo sapiens 48 R-HSA-5693549 ATM phosphorylates histone H2AFX on S139 at DNA DSBs yes reactome/R-HSA-5693549.md ATM phosphorylates histone H2AFX on S139 at DNA DSBs Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Retain supported protein serine/threonine kinase activity. reaction_mf_exact accepted_mf 1 human ATM genes/human/ATM/ATM-ai-review.yaml ATM Q13315 NCBITaxon:9606 Homo sapiens 49 R-HSA-5693551 Phosphorylation of BRCA1-A complex at multiple sites by ATM yes reactome/R-HSA-5693551.md Phosphorylation of BRCA1-A complex at multiple sites by ATM Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Retain supported protein serine/threonine kinase activity. reaction_mf_exact accepted_mf 1 human ATM genes/human/ATM/ATM-ai-review.yaml ATM Q13315 NCBITaxon:9606 Homo sapiens 50 R-HSA-5693598 ATM phosphorylates NBN yes reactome/R-HSA-5693598.md ATM phosphorylates NBN Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Retain supported protein serine/threonine kinase activity. reaction_mf_exact accepted_mf 1 human ATM genes/human/ATM/ATM-ai-review.yaml ATM Q13315 NCBITaxon:9606 Homo sapiens 51 R-HSA-5693609 ATM phosphorylates TP53 at S15 yes reactome/R-HSA-5693609.md ATM phosphorylates TP53 at S15 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Retain supported protein serine/threonine kinase activity. reaction_mf_exact accepted_mf 1 human ATM genes/human/ATM/ATM-ai-review.yaml ATM Q13315 NCBITaxon:9606 Homo sapiens 52 R-HSA-6798372 ATM phosphorylates DYRK2 yes reactome/R-HSA-6798372.md ATM phosphorylates DYRK2 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Retain source-supported protein serine/threonine kinase activity. reaction_mf_exact accepted_mf 1 human ATM genes/human/ATM/ATM-ai-review.yaml ATM Q13315 NCBITaxon:9606 Homo sapiens 53 R-HSA-6799097 ATM phosphorylates ZNF420 yes reactome/R-HSA-6799097.md ATM phosphorylates ZNF420 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Retain source-supported protein serine/threonine kinase activity. reaction_mf_exact accepted_mf 1 human ATM genes/human/ATM/ATM-ai-review.yaml ATM Q13315 NCBITaxon:9606 Homo sapiens 54 R-HSA-6800490 ATM phosphorylates PIDD1 yes reactome/R-HSA-6800490.md ATM phosphorylates PIDD1 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Retain source-supported protein serine/threonine kinase activity. reaction_mf_exact accepted_mf 1 human ATM genes/human/ATM/ATM-ai-review.yaml ATM Q13315 NCBITaxon:9606 Homo sapiens 55 R-HSA-6804276 ATM phosphorylates TTC5 yes reactome/R-HSA-6804276.md ATM phosphorylates TTC5 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Retain source-supported protein serine/threonine kinase activity. reaction_mf_exact accepted_mf 1 human ATM genes/human/ATM/ATM-ai-review.yaml ATM Q13315 NCBITaxon:9606 Homo sapiens 56 R-HSA-6804955 ATM phosphorylates MDM2 yes reactome/R-HSA-6804955.md ATM phosphorylates MDM2 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Retain source-supported protein serine/threonine kinase activity. reaction_mf_exact accepted_mf 1 human ATM genes/human/ATM/ATM-ai-review.yaml ATM Q13315 NCBITaxon:9606 Homo sapiens 57 R-HSA-69891 Phosphorylation and activation of CHEK2 by ATM yes reactome/R-HSA-69891.md Phosphorylation and activation of CHEK2 by ATM Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Retain supported protein serine/threonine kinase activity. reaction_mf_exact accepted_mf 1 human ATP2B2 genes/human/ATP2B2/ATP2B2-ai-review.yaml ATP2B2 Q01814 NCBITaxon:9606 Homo sapiens 7 R-HSA-418309 ATP2B1-4 transport cytosolic Ca2+ to extracellular region yes reactome/R-HSA-418309.md ATP2B1-4 transport cytosolic Ca2+ to extracellular region Homo sapiens GO:0005388 P-type calcium transporter activity TAS enables MF false ACCEPT Reactome places PMCA2 among plasma-membrane calcium-exporting ATPases. reaction_mf_exact accepted_mf 1 human ATP7B genes/human/ATP7B/ATP7B-ai-review.yaml ATP7B P35670 NCBITaxon:9606 Homo sapiens 6 R-HSA-936895 ATP7B transports cytosolic Cu1+ to Golgi lumen yes reactome/R-HSA-936895.md ATP7B transports cytosolic Cu1+ to Golgi lumen Homo sapiens GO:0140581 P-type monovalent copper transporter activity TAS enables MF false ACCEPT Reactome pathway annotation for ATP7B transporting Cu1+ to Golgi lumen. Redundant with IMP evidence. reaction_mf_exact accepted_mf 1 human ATXN3 genes/human/ATXN3/ATXN3-ai-review.yaml ATXN3 P54252 NCBITaxon:9606 Homo sapiens 23 R-HSA-5688797 ATXN3 family cleave Ub chains yes reactome/R-HSA-5688797.md ATXN3 family cleave Ub chains Homo sapiens GO:0004843 cysteine-type deubiquitinase activity TAS enables MF false ACCEPT Reactome (TAS) assertion of cysteine-type deubiquitinase activity (Ub-chain cleavage). reaction_mf_exact accepted_mf 1 human ATXN3 genes/human/ATXN3/ATXN3-ai-review.yaml ATXN3 P54252 NCBITaxon:9606 Homo sapiens 24 R-HSA-5688837 ATXN3 deubiquitinates polyUb-PARK2 yes reactome/R-HSA-5688837.md ATXN3 deubiquitinates polyUb-PARK2 Homo sapiens GO:0004843 cysteine-type deubiquitinase activity TAS enables MF false ACCEPT Reactome (TAS) assertion of DUB activity in the context of deubiquitinating polyUb-parkin (PARK2). reaction_mf_exact accepted_mf 1 human B3GALNT1 genes/human/B3GALNT1/B3GALNT1-ai-review.yaml B3GALNT1 O75752 NCBITaxon:9606 Homo sapiens 11 R-HSA-8878914 B3GALNT1 transfers GalNAc to Gb3Cer to form Gb4Cer yes reactome/R-HSA-8878914.md B3GALNT1 transfers GalNAc to Gb3Cer to form Gb4Cer Homo sapiens GO:0047273 galactosylgalactosylglucosylceramide beta-D-acetylgalactosaminyltransferase activity TAS enables MF false ACCEPT "Reactome reaction ""B3GALNT1 transfers GalNAc to Gb3Cer to form Gb4Cer"" directly supports the globoside synthase MF." reaction_mf_exact accepted_mf 1 human B3GALNT2 genes/human/B3GALNT2/B3GALNT2-ai-review.yaml B3GALNT2 Q8NCR0 NCBITaxon:9606 Homo sapiens 11 R-HSA-8931648 B3GALNT2 transfers GalNAc to GlcNAc-Man-DAG1 yes reactome/R-HSA-8931648.md B3GALNT2 transfers GalNAc to GlcNAc-Man-DAG1 Homo sapiens GO:0008376 acetylgalactosaminyltransferase activity TAS enables MF false ACCEPT "Correct and specific molecular function from the Reactome reaction ""B3GALNT2 transfers GalNAc to GlcNAc-Man-DAG1"". This is the core catalytic activity of the enzyme. Accept (concordant with the IDA annotation)." reaction_mf_exact accepted_mf 1 human B3GALT6 genes/human/B3GALT6/B3GALT6-ai-review.yaml B3GALT6 Q96L58 NCBITaxon:9606 Homo sapiens 25 R-HSA-1889978 B3GALT6 transfers Gal to the tetrasaccharide linker yes reactome/R-HSA-1889978.md B3GALT6 transfers Gal to the tetrasaccharide linker Homo sapiens GO:0047220 galactosylxylosylprotein 3-beta-galactosyltransferase activity TAS enables MF false ACCEPT The normal Reactome reaction matches GalT-II catalysis. reaction_mf_exact accepted_mf 1 human B3GALT6 genes/human/B3GALT6/B3GALT6-ai-review.yaml B3GALT6 Q96L58 NCBITaxon:9606 Homo sapiens 26 R-HSA-4420365 Defective B3GALT6 does not transfer Gal to the tetrasaccharide linker yes reactome/R-HSA-4420365.md Defective B3GALT6 does not transfer Gal to the tetrasaccharide linker Homo sapiens GO:0047220 galactosylxylosylprotein 3-beta-galactosyltransferase activity TAS enables MF false ACCEPT The disease event identifies disruption of the normal GalT-II reaction. reaction_mf_exact accepted_mf reactome_disease_event 1 human B3GNT5 genes/human/B3GNT5/B3GNT5-ai-review.yaml B3GNT5 Q9BYG0 NCBITaxon:9606 Homo sapiens 12 R-HSA-9846501 B3GNT5 transfers GlcNAc to LacCer yes reactome/R-HSA-9846501.md B3GNT5 transfers GlcNAc to LacCer Homo sapiens GO:0047256 lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase activity TAS enables MF false ACCEPT "Precise core molecular function, curated by Reactome from the reaction ""B3GNT5 transfers GlcNAc to LacCer"". Matches the experimental EC 2.4.1.206 activity. Accepted." reaction_mf_exact accepted_mf 1 human B4GALT1 genes/human/B4GALT1/B4GALT1-ai-review.yaml B4GALT1 P15291 NCBITaxon:9606 Homo sapiens 19 R-HSA-3656230 Defective B4GALT1 does not transfer Gal to the keratan chain yes reactome/R-HSA-3656230.md Defective B4GALT1 does not transfer Gal to the keratan chain Homo sapiens GO:0003831 beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity TAS enables MF false ACCEPT "Reactome reaction (""Defective B4GALT1 does not transfer Gal to the keratan chain"") annotating the core galactosyltransferase activity (here in the keratan sulfate context). Correct core MF. Accept." reaction_mf_exact accepted_mf reactome_disease_event 1 human B4GALT1 genes/human/B4GALT1/B4GALT1-ai-review.yaml B4GALT1 P15291 NCBITaxon:9606 Homo sapiens 20 R-HSA-4793956 Defective B4GALT1 does not add Gal to N-glycan yes reactome/R-HSA-4793956.md Defective B4GALT1 does not add Gal to N-glycan Homo sapiens GO:0003831 beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity TAS enables MF false ACCEPT "Reactome reaction (""Defective B4GALT1 does not add Gal to N-glycan"") annotating the core N-glycan galactosyltransferase activity. Correct core MF. Accept." reaction_mf_exact accepted_mf reactome_disease_event 1 human B4GALT1 genes/human/B4GALT1/B4GALT1-ai-review.yaml B4GALT1 P15291 NCBITaxon:9606 Homo sapiens 21 R-HSA-9035949 Defective B4GALT1 does not transfer Gal to the N-glycan precursor yes reactome/R-HSA-9035949.md Defective B4GALT1 does not transfer Gal to the N-glycan precursor Homo sapiens GO:0003831 beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity TAS enables MF false ACCEPT Reactome reaction annotating the core galactosyltransferase activity (N-glycan precursor context). Redundant with the other MF annotations; correct. Accept. reaction_mf_exact accepted_mf reactome_disease_event 1 human B4GALT1 genes/human/B4GALT1/B4GALT1-ai-review.yaml B4GALT1 P15291 NCBITaxon:9606 Homo sapiens 22 R-HSA-9035950 Defective B4GALT1 does not transfer Gal to a branch of keratan yes reactome/R-HSA-9035950.md Defective B4GALT1 does not transfer Gal to a branch of keratan Homo sapiens GO:0003831 beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity TAS enables MF false ACCEPT Reactome reaction annotating the core galactosyltransferase activity (keratan branch context). Correct core MF. Accept. reaction_mf_exact accepted_mf reactome_disease_event 1 human B4GALT7 genes/human/B4GALT7/B4GALT7-ai-review.yaml B4GALT7 Q9UBV7 NCBITaxon:9606 Homo sapiens 35 R-HSA-1889981 B4GALT7 transfers Gal group to xylosyl-unit of the tetrasaccharide linker yes reactome/R-HSA-1889981.md B4GALT7 transfers Gal group to xylosyl-unit of the tetrasaccharide linker Homo sapiens GO:0046525 xylosylprotein 4-beta-galactosyltransferase activity TAS enables MF false ACCEPT B4GALT7 catalyzes the xylose-directed first galactose addition. reaction_mf_exact accepted_mf 1 human B4GALT7 genes/human/B4GALT7/B4GALT7-ai-review.yaml B4GALT7 Q9UBV7 NCBITaxon:9606 Homo sapiens 36 R-HSA-3560804 Defective B4GALT7 does not transfer Gal to xylosyl-unit of the tetrasaccharide linker yes reactome/R-HSA-3560804.md Defective B4GALT7 does not transfer Gal to xylosyl-unit of the tetrasaccharide linker Homo sapiens GO:0046525 xylosylprotein 4-beta-galactosyltransferase activity TAS enables MF false ACCEPT B4GALT7 catalyzes the xylose-directed first galactose addition. reaction_mf_exact accepted_mf reactome_disease_event 1 human BACE1 genes/human/BACE1/BACE1-ai-review.yaml BACE1 P56817 NCBITaxon:9606 Homo sapiens 59 R-HSA-5692495 BACE1 cleaves APP(18-770) to APP(18-671) and APP(672-770) yes reactome/R-HSA-5692495.md BACE1 cleaves APP(18-770) to APP(18-671) and APP(672-770) Homo sapiens GO:0008798 beta-aspartyl-peptidase activity TAS enables MF false ACCEPT beta-aspartyl-peptidase activity is retained as a core molecular-function annotation for BACE1; it captures activity or binding specificity within the synthesized core biology: BACE1 beta-secretase/aspartyl endopeptidase activity, amyloidogenic APP processing, membrane-substrate proteolysis, and TGN/endosomal trafficking that controls substrate access and enzyme turnover. reaction_mf_exact accepted_mf 1 human BAP1 genes/human/BAP1/BAP1-ai-review.yaml BAP1 Q92560 NCBITaxon:9606 Homo sapiens 17 R-HSA-5690759 BAP1:Ub-HCFC1 deubiquitinates BAP1:Ub-HCFC1 yes reactome/R-HSA-5690759.md BAP1:Ub-HCFC1 deubiquitinates BAP1:Ub-HCFC1 Homo sapiens GO:0004843 cysteine-type deubiquitinase activity TAS enables MF false ACCEPT Reactome assigns BAP1 the catalytic role in HCF-1 deubiquitination. reaction_mf_exact accepted_mf 1 human BAP1 genes/human/BAP1/BAP1-ai-review.yaml BAP1 Q92560 NCBITaxon:9606 Homo sapiens 18 R-HSA-5690790 Histone H2A is dubiquitinated by the PR-DUB complex yes reactome/R-HSA-5690790.md Histone H2A is dubiquitinated by the PR-DUB complex Homo sapiens GO:0004843 cysteine-type deubiquitinase activity TAS enables MF false ACCEPT BAP1 supplies the deubiquitinase chemistry in the Reactome PR-DUB reaction. reaction_mf_exact accepted_mf 1 human BPGM genes/human/BPGM/BPGM-ai-review.yaml BPGM P07738 NCBITaxon:9606 Homo sapiens 12 R-HSA-6798335 BPGM dimer isomerises 1,3BPG to 2,3BPG yes reactome/R-HSA-6798335.md BPGM dimer isomerises 1,3BPG to 2,3BPG Homo sapiens GO:0004082 bisphosphoglycerate mutase activity TAS enables MF false ACCEPT Reactome-traceable assertion of bisphosphoglycerate mutase activity (isomerisation of 1,3-BPG to 2,3-BPG). This is the core molecular function of BPGM. reaction_mf_exact accepted_mf local_module_mf 1 lactate_fermentation_and_bpg_shunt:GO:0004082 bisphosphoglycerate mutase activity human BRCA1 genes/human/BRCA1/BRCA1-ai-review.yaml BRCA1 P38398 NCBITaxon:9606 Homo sapiens 47 R-HSA-9701000 BRCA1:BARD1 heterodimer autoubiquitinates yes reactome/R-HSA-9701000.md BRCA1:BARD1 heterodimer autoubiquitinates Homo sapiens GO:0004842 ubiquitin-protein transferase activity TAS enables MF false ACCEPT Reactome pathway annotation for BRCA1-BARD1 autoubiquitination activity. reaction_mf_exact accepted_mf 1 human BTD genes/human/BTD/BTD-ai-review.yaml BTD P43251 NCBITaxon:9606 Homo sapiens 10 R-HSA-3076905 Extracellular BTD hydrolyses BCTN no GO:0047708 biotinidase activity TAS enables MF false ACCEPT Reactome-asserted biotinidase activity for the extracellular hydrolysis of biocytin; the core molecular function. reaction_mf_exact accepted_mf local_module_mf|uncached_reactome 1 biotin_cycle:GO:0047708 biotinidase activity human BTD genes/human/BTD/BTD-ai-review.yaml BTD P43251 NCBITaxon:9606 Homo sapiens 11 R-HSA-3325540 Defective extracellular BTD does not hydrolyse BCTN no GO:0047708 biotinidase activity TAS enables MF false ACCEPT "Biotinidase activity annotation from the Reactome ""defective extracellular BTD"" disease reaction; the term is the correct core molecular function (its loss is what defines the disease reaction)." reaction_mf_exact accepted_mf reactome_disease_event|uncached_reactome 1 human BTD genes/human/BTD/BTD-ai-review.yaml BTD P43251 NCBITaxon:9606 Homo sapiens 12 R-HSA-4167509 Mitochondrial BTD hydrolyses BCTN no GO:0047708 biotinidase activity TAS enables MF false ACCEPT "Biotinidase activity annotation from the Reactome ""mitochondrial BTD hydrolyses BCTN"" reaction; the molecular function is correct and core." reaction_mf_exact accepted_mf uncached_reactome 1 human BTD genes/human/BTD/BTD-ai-review.yaml BTD P43251 NCBITaxon:9606 Homo sapiens 13 R-HSA-4225086 Defective mitochondrial BTD does not hydrolyse BCTN no GO:0047708 biotinidase activity TAS enables MF false ACCEPT "Biotinidase activity annotation from the Reactome ""defective mitochondrial BTD"" disease reaction; the referenced molecular function is correct and core." reaction_mf_exact accepted_mf reactome_disease_event|uncached_reactome 1 human CANX genes/human/CANX/CANX-ai-review.yaml CANX P27824 NCBITaxon:9606 Homo sapiens 36 R-HSA-9683772 Trimmed spike protein binds to calnexin yes reactome/R-HSA-9683772.md Trimmed spike protein binds to calnexin Homo sapiens GO:0044183 protein folding chaperone TAS enables MF false ACCEPT Protein folding chaperone is an accurate core molecular function for calnexin. reaction_mf_exact accepted_mf 1 human CASP4 genes/human/CASP4/CASP4-ai-review.yaml CASP4 P49662 NCBITaxon:9606 Homo sapiens 23 R-HSA-9947861 CASP4 autocatalysis on LPS micelles yes reactome/R-HSA-9947861.md CASP4 autocatalysis on LPS micelles Homo sapiens GO:0004197 cysteine-type endopeptidase activity TAS enables MF false ACCEPT CASP4 is a cysteine protease that cleaves after Asp; this is its uncontested core molecular function. reaction_mf_exact accepted_mf 1 human CASP9 genes/human/CASP9/CASP9-ai-review.yaml CASP9 P55211 NCBITaxon:9606 Homo sapiens 65 R-HSA-114259 Cleavage of Procaspase‑9 to caspase‑9 yes reactome/R-HSA-114259.md Cleavage of Procaspase‑9 to caspase‑9 Homo sapiens GO:0004197 cysteine-type endopeptidase activity TAS enables MF false ACCEPT Reactome pathway annotation for caspase-9 cleavage. CRITICAL: This catalytic activity is ONLY present in isoform 1. reaction_mf_exact accepted_mf 1 human CDC25B genes/human/CDC25B/CDC25B-ai-review.yaml CDC25B P30305 NCBITaxon:9606 Homo sapiens 32 R-HSA-170153 Dephosphorylation of nuclear Cyclin B1:phospho-Cdc2 (Thr 14, Tyr15) complexes by Cdc25 phosphatases yes reactome/R-HSA-170153.md Dephosphorylation of nuclear Cyclin B1:phospho-Cdc2 (Thr 14, Tyr15) complexes by Cdc25 phosphatases Homo sapiens GO:0004721 phosphoprotein phosphatase activity TAS enables MF false ACCEPT TAS from Reactome pathway for dephosphorylation of nuclear Cyclin B1:phospho-Cdc2 complexes by Cdc25 phosphatases. reaction_mf_exact accepted_mf multi_gene_reactome_id 2 human CDC25B genes/human/CDC25B/CDC25B-ai-review.yaml CDC25B P30305 NCBITaxon:9606 Homo sapiens 33 R-HSA-170161 Dephosphorylation of cytoplasmic Cyclin B1/B2:phospho-Cdc2 (Thr 14, Tyr 15) complexes by CDC25B yes reactome/R-HSA-170161.md Dephosphorylation of cytoplasmic Cyclin B1/B2:phospho-Cdc2 (Thr 14, Tyr 15) complexes by CDC25B Homo sapiens GO:0004721 phosphoprotein phosphatase activity TAS enables MF false ACCEPT TAS from Reactome pathway for dephosphorylation of cytoplasmic Cyclin B1/B2: phospho-Cdc2 complexes specifically by CDC25B. reaction_mf_exact accepted_mf 1 human CDC25C genes/human/CDC25C/CDC25C-ai-review.yaml CDC25C P30307 NCBITaxon:9606 Homo sapiens 6 R-HSA-170153 Dephosphorylation of nuclear Cyclin B1:phospho-Cdc2 (Thr 14, Tyr15) complexes by Cdc25 phosphatases yes reactome/R-HSA-170153.md Dephosphorylation of nuclear Cyclin B1:phospho-Cdc2 (Thr 14, Tyr15) complexes by Cdc25 phosphatases Homo sapiens GO:0004721 phosphoprotein phosphatase activity TAS enables MF false ACCEPT Reactome TAS for dephosphorylation of nuclear cyclin B1:phospho-CDK1 (Thr14/Tyr15) by CDC25 phosphatases. reaction_mf_exact accepted_mf multi_gene_reactome_id 2 human CDK1 genes/human/CDK1/CDK1-ai-review.yaml CDK1 P06493 NCBITaxon:9606 Homo sapiens 93 R-HSA-112342 Inactivation of MAP2K1 by CDK1 yes reactome/R-HSA-112342.md Inactivation of MAP2K1 by CDK1 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT CDK1 is a serine/threonine kinase that phosphorylates S/T-P motifs. This is well-established biochemically. reaction_mf_exact accepted_mf 1 human CDK1 genes/human/CDK1/CDK1-ai-review.yaml CDK1 P06493 NCBITaxon:9606 Homo sapiens 94 R-HSA-8940100 CDK1 phosphorylates VCPIP1 yes reactome/R-HSA-8940100.md CDK1 phosphorylates VCPIP1 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT CDK1 is a serine/threonine kinase that phosphorylates S/T-P motifs. This is well-established biochemically. reaction_mf_exact accepted_mf 1 human CDK1 genes/human/CDK1/CDK1-ai-review.yaml CDK1 P06493 NCBITaxon:9606 Homo sapiens 95 R-HSA-4088024 CCNA:CDK1/2 complexes and CCNB1:CDK1 complexes phosphorylate FOXM1 yes reactome/R-HSA-4088024.md CCNA:CDK1/2 complexes and CCNB1:CDK1 complexes phosphorylate FOXM1 Homo sapiens GO:0004693 cyclin-dependent protein serine/threonine kinase activity TAS enables MF false ACCEPT This is the most appropriate molecular function term for CDK1. The enzyme requires cyclin binding for activity and phosphorylates serine/threonine residues. reaction_mf_exact accepted_mf 1 human CDK1 genes/human/CDK1/CDK1-ai-review.yaml CDK1 P06493 NCBITaxon:9606 Homo sapiens 96 R-HSA-5692755 CDK1 phosphorylates MAPK6 yes reactome/R-HSA-5692755.md CDK1 phosphorylates MAPK6 Homo sapiens GO:0004693 cyclin-dependent protein serine/threonine kinase activity TAS enables MF false ACCEPT This is the most appropriate molecular function term for CDK1. The enzyme requires cyclin binding for activity and phosphorylates serine/threonine residues. reaction_mf_exact accepted_mf 1 human CDK1 genes/human/CDK1/CDK1-ai-review.yaml CDK1 P06493 NCBITaxon:9606 Homo sapiens 97 R-HSA-9929514 CDK1 phosphorylates CCNA yes reactome/R-HSA-9929514.md CDK1 phosphorylates CCNA Homo sapiens GO:0004693 cyclin-dependent protein serine/threonine kinase activity TAS enables MF false ACCEPT This is the most appropriate molecular function term for CDK1. The enzyme requires cyclin binding for activity and phosphorylates serine/threonine residues. reaction_mf_exact accepted_mf 1 human CDK1 genes/human/CDK1/CDK1-ai-review.yaml CDK1 P06493 NCBITaxon:9606 Homo sapiens 98 R-HSA-9929532 CCNA:CDK1 phosphorylates BORA yes reactome/R-HSA-9929532.md CCNA:CDK1 phosphorylates BORA Homo sapiens GO:0004693 cyclin-dependent protein serine/threonine kinase activity TAS enables MF false ACCEPT This is the most appropriate molecular function term for CDK1. The enzyme requires cyclin binding for activity and phosphorylates serine/threonine residues. reaction_mf_exact accepted_mf 1 human CDK1 genes/human/CDK1/CDK1-ai-review.yaml CDK1 P06493 NCBITaxon:9606 Homo sapiens 99 R-HSA-9929535 CCNA:CDK1 phosphorylates WEE1 yes reactome/R-HSA-9929535.md CCNA:CDK1 phosphorylates WEE1 Homo sapiens GO:0004693 cyclin-dependent protein serine/threonine kinase activity TAS enables MF false ACCEPT This is the most appropriate molecular function term for CDK1. The enzyme requires cyclin binding for activity and phosphorylates serine/threonine residues. reaction_mf_exact accepted_mf 1 human CDK1 genes/human/CDK1/CDK1-ai-review.yaml CDK1 P06493 NCBITaxon:9606 Homo sapiens 100 R-HSA-9929717 CCNA:CDK1 phosphorylates FZR1 yes reactome/R-HSA-9929717.md CCNA:CDK1 phosphorylates FZR1 Homo sapiens GO:0004693 cyclin-dependent protein serine/threonine kinase activity TAS enables MF false ACCEPT This is the most appropriate molecular function term for CDK1. The enzyme requires cyclin binding for activity and phosphorylates serine/threonine residues. reaction_mf_exact accepted_mf 1 human CDK1 genes/human/CDK1/CDK1-ai-review.yaml CDK1 P06493 NCBITaxon:9606 Homo sapiens 101 R-HSA-9929719 CCNA:CDK1 phosphorylates TICRR yes reactome/R-HSA-9929719.md CCNA:CDK1 phosphorylates TICRR Homo sapiens GO:0004693 cyclin-dependent protein serine/threonine kinase activity TAS enables MF false ACCEPT This is the most appropriate molecular function term for CDK1. The enzyme requires cyclin binding for activity and phosphorylates serine/threonine residues. reaction_mf_exact accepted_mf 1 human CDK1 genes/human/CDK1/CDK1-ai-review.yaml CDK1 P06493 NCBITaxon:9606 Homo sapiens 102 R-HSA-9929720 CCNA:CDK1 phosphorylates OBI1 yes reactome/R-HSA-9929720.md CCNA:CDK1 phosphorylates OBI1 Homo sapiens GO:0004693 cyclin-dependent protein serine/threonine kinase activity TAS enables MF false ACCEPT This is the most appropriate molecular function term for CDK1. The enzyme requires cyclin binding for activity and phosphorylates serine/threonine residues. reaction_mf_exact accepted_mf 1 human CDK1 genes/human/CDK1/CDK1-ai-review.yaml CDK1 P06493 NCBITaxon:9606 Homo sapiens 103 R-HSA-9929721 CCNA:CDK1 phosphorylates SGO1 yes reactome/R-HSA-9929721.md CCNA:CDK1 phosphorylates SGO1 Homo sapiens GO:0004693 cyclin-dependent protein serine/threonine kinase activity TAS enables MF false ACCEPT This is the most appropriate molecular function term for CDK1. The enzyme requires cyclin binding for activity and phosphorylates serine/threonine residues. reaction_mf_exact accepted_mf 1 human CDK1 genes/human/CDK1/CDK1-ai-review.yaml CDK1 P06493 NCBITaxon:9606 Homo sapiens 104 R-HSA-9929884 CCNA:CDK1 phosphorylates HJURP yes reactome/R-HSA-9929884.md CCNA:CDK1 phosphorylates HJURP Homo sapiens GO:0004693 cyclin-dependent protein serine/threonine kinase activity TAS enables MF false ACCEPT This is the most appropriate molecular function term for CDK1. The enzyme requires cyclin binding for activity and phosphorylates serine/threonine residues. reaction_mf_exact accepted_mf 1 human CDK1 genes/human/CDK1/CDK1-ai-review.yaml CDK1 P06493 NCBITaxon:9606 Homo sapiens 105 R-HSA-9929904 CCNA:CDK1 phosphorylates MIS18BP1 yes reactome/R-HSA-9929904.md CCNA:CDK1 phosphorylates MIS18BP1 Homo sapiens GO:0004693 cyclin-dependent protein serine/threonine kinase activity TAS enables MF false ACCEPT This is the most appropriate molecular function term for CDK1. The enzyme requires cyclin binding for activity and phosphorylates serine/threonine residues. reaction_mf_exact accepted_mf 1 human CDK1 genes/human/CDK1/CDK1-ai-review.yaml CDK1 P06493 NCBITaxon:9606 Homo sapiens 106 R-HSA-9929935 CCNA:CDK1 phosphorylates CDC25B yes reactome/R-HSA-9929935.md CCNA:CDK1 phosphorylates CDC25B Homo sapiens GO:0004693 cyclin-dependent protein serine/threonine kinase activity TAS enables MF false ACCEPT This is the most appropriate molecular function term for CDK1. The enzyme requires cyclin binding for activity and phosphorylates serine/threonine residues. reaction_mf_exact accepted_mf 1 human CDK2 genes/human/CDK2/CDK2-ai-review.yaml CDK2 P24941 NCBITaxon:9606 Homo sapiens 96 R-HSA-174079 Reactome:R-HSA-174079 yes reactome/R-HSA-174079.md Phosphorylation of Cdh1 by Cyclin A:Cdk2 Homo sapiens GO:0004693 cyclin-dependent protein serine/threonine kinase activity TAS enables MF false ACCEPT CDK2 is a cyclin-dependent serine/threonine protein kinase (EC 2.7.11.22); this is its defining, well-established molecular function, active only when bound to a cyclin partner. reaction_mf_exact accepted_mf 1 human CDK2 genes/human/CDK2/CDK2-ai-review.yaml CDK2 P24941 NCBITaxon:9606 Homo sapiens 97 R-HSA-187520 Reactome:R-HSA-187520 yes reactome/R-HSA-187520.md Cyclin E/A:Cdk2-mediated phosphorylation of p27/p21 Homo sapiens GO:0004693 cyclin-dependent protein serine/threonine kinase activity TAS enables MF false ACCEPT CDK2 is a cyclin-dependent serine/threonine protein kinase (EC 2.7.11.22); this is its defining, well-established molecular function, active only when bound to a cyclin partner. reaction_mf_exact accepted_mf 1 human CDK2 genes/human/CDK2/CDK2-ai-review.yaml CDK2 P24941 NCBITaxon:9606 Homo sapiens 98 R-HSA-187959 Reactome:R-HSA-187959 yes reactome/R-HSA-187959.md Phosphorylation of E2F1/E2F3 by Cyclin A:phosph-Cdk2(Thr 160) Homo sapiens GO:0004693 cyclin-dependent protein serine/threonine kinase activity TAS enables MF false ACCEPT CDK2 is a cyclin-dependent serine/threonine protein kinase (EC 2.7.11.22); this is its defining, well-established molecular function, active only when bound to a cyclin partner. reaction_mf_exact accepted_mf 1 human CDK2 genes/human/CDK2/CDK2-ai-review.yaml CDK2 P24941 NCBITaxon:9606 Homo sapiens 99 R-HSA-188390 Reactome:R-HSA-188390 yes reactome/R-HSA-188390.md Cyclin E:CDK2-mediated phosphorylation of RB1 Homo sapiens GO:0004693 cyclin-dependent protein serine/threonine kinase activity TAS enables MF false ACCEPT CDK2 is a cyclin-dependent serine/threonine protein kinase (EC 2.7.11.22); this is its defining, well-established molecular function, active only when bound to a cyclin partner. reaction_mf_exact accepted_mf 1 human CDK2 genes/human/CDK2/CDK2-ai-review.yaml CDK2 P24941 NCBITaxon:9606 Homo sapiens 100 R-HSA-3788705 Reactome:R-HSA-3788705 yes reactome/R-HSA-3788705.md CDKN1A (p21) prevents phosphorylation of Cdh1 by Cyclin A:Cdk2 Homo sapiens GO:0004693 cyclin-dependent protein serine/threonine kinase activity TAS enables MF false ACCEPT CDK2 is a cyclin-dependent serine/threonine protein kinase (EC 2.7.11.22); this is its defining, well-established molecular function, active only when bound to a cyclin partner. reaction_mf_exact accepted_mf 1 human CDK4 genes/human/CDK4/CDK4-ai-review.yaml CDK4 P11802 NCBITaxon:9606 Homo sapiens 15 R-HSA-9008412 CDK4 phosphorylates RUNX2 yes reactome/R-HSA-9008412.md CDK4 phosphorylates RUNX2 Homo sapiens GO:0004693 cyclin-dependent protein serine/threonine kinase activity TAS enables MF false ACCEPT Cyclin-dependent protein serine/threonine kinase activity (EC 2.7.11.22) - CDK4's defining molecular function, active as the cyclin D-CDK4 holoenzyme. reaction_mf_exact accepted_mf 1 human CDK4 genes/human/CDK4/CDK4-ai-review.yaml CDK4 P11802 NCBITaxon:9606 Homo sapiens 16 R-HSA-9929482 CDK4:CCND1 phosphorylates SPOP yes reactome/R-HSA-9929482.md CDK4:CCND1 phosphorylates SPOP Homo sapiens GO:0004693 cyclin-dependent protein serine/threonine kinase activity TAS enables MF false ACCEPT Cyclin-dependent protein serine/threonine kinase activity (EC 2.7.11.22) - CDK4's defining molecular function, active as the cyclin D-CDK4 holoenzyme. reaction_mf_exact accepted_mf 1 human CDK5 genes/human/CDK5/CDK5-ai-review.yaml CDK5 Q00535 NCBITaxon:9606 Homo sapiens 68 R-HSA-399944 Phosphorylation of CRMPs by Cdk5 yes reactome/R-HSA-399944.md Phosphorylation of CRMPs by Cdk5 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT protein serine/threonine kinase activity is a core CDK5 molecular function. reaction_mf_exact accepted_mf 1 human CDK5 genes/human/CDK5/CDK5-ai-review.yaml CDK5 Q00535 NCBITaxon:9606 Homo sapiens 69 R-HSA-8863007 p25-bound CDK5 phosphorylates CDC25B yes reactome/R-HSA-8863007.md p25-bound CDK5 phosphorylates CDC25B Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT protein serine/threonine kinase activity is a core CDK5 molecular function. reaction_mf_exact accepted_mf 1 human CDK5 genes/human/CDK5/CDK5-ai-review.yaml CDK5 Q00535 NCBITaxon:9606 Homo sapiens 70 R-HSA-8863011 p25-bound CDK5 phosphorylates CDC25C yes reactome/R-HSA-8863011.md p25-bound CDK5 phosphorylates CDC25C Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT protein serine/threonine kinase activity is a core CDK5 molecular function. reaction_mf_exact accepted_mf 1 human CDK5 genes/human/CDK5/CDK5-ai-review.yaml CDK5 Q00535 NCBITaxon:9606 Homo sapiens 71 R-HSA-8863014 p25-bound CDK5 phosphorylates CDC25A yes reactome/R-HSA-8863014.md p25-bound CDK5 phosphorylates CDC25A Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT protein serine/threonine kinase activity is a core CDK5 molecular function. reaction_mf_exact accepted_mf 1 human CDK5 genes/human/CDK5/CDK5-ai-review.yaml CDK5 Q00535 NCBITaxon:9606 Homo sapiens 72 R-HSA-180047 CDK5 phosphorylates DARPP-32 on Thr75 yes reactome/R-HSA-180047.md CDK5 phosphorylates DARPP-32 on Thr75 Homo sapiens GO:0004693 cyclin-dependent protein serine/threonine kinase activity TAS enables MF false ACCEPT cyclin-dependent protein serine/threonine kinase activity is a core CDK5 molecular function. reaction_mf_exact accepted_mf 1 human CDKN1B genes/human/CDKN1B/CDKN1B-ai-review.yaml CDKN1B P46527 NCBITaxon:9606 Homo sapiens 12 R-HSA-187934 Inactivation of Cyclin A:Cdk2 complexes by p27/p21 yes reactome/R-HSA-187934.md Inactivation of Cyclin A:Cdk2 complexes by p27/p21 Homo sapiens GO:0004861 cyclin-dependent protein serine/threonine kinase inhibitor activity TAS enables MF false ACCEPT CDK inhibitor activity asserted by the Reactome reaction 'Inactivation of Cyclin A:Cdk2 complexes by p27/p21'. reaction_mf_exact accepted_mf 1 human CDKN1B genes/human/CDKN1B/CDKN1B-ai-review.yaml CDKN1B P46527 NCBITaxon:9606 Homo sapiens 13 R-HSA-69562 Inactivation of Cyclin E:Cdk2 complexes by p27/p21 yes reactome/R-HSA-69562.md Inactivation of Cyclin E:Cdk2 complexes by p27/p21 Homo sapiens GO:0004861 cyclin-dependent protein serine/threonine kinase inhibitor activity TAS enables MF false ACCEPT CDK inhibitor activity asserted by the Reactome reaction 'Inactivation of Cyclin E:Cdk2 complexes by p27/p21'. reaction_mf_exact accepted_mf 1 human CERS1 genes/human/CERS1/CERS1-ai-review.yaml CERS1 P27544 NCBITaxon:9606 Homo sapiens 16 R-HSA-428185 Ceramide synthases transfer acyl-CoA onto sphingoid yes reactome/R-HSA-428185.md Ceramide synthases transfer acyl-CoA onto sphingoid Homo sapiens GO:0050291 sphingosine N-acyltransferase activity TAS enables MF false ACCEPT Reactome (Ceramide synthases transfer acyl-CoA onto sphingoid) traceable assignment of the core ceramide-synthase catalytic activity. reaction_mf_exact accepted_mf local_module_mf|multi_gene_reactome_id 3 sphingolipid_de_novo_synthesis:GO:0050291 sphingosine N-acyltransferase activity human CERS2 genes/human/CERS2/CERS2-ai-review.yaml CERS2 Q96G23 NCBITaxon:9606 Homo sapiens 22 R-HSA-428185 Ceramide synthases transfer acyl-CoA onto sphingoid yes reactome/R-HSA-428185.md Ceramide synthases transfer acyl-CoA onto sphingoid Homo sapiens GO:0050291 sphingosine N-acyltransferase activity TAS enables MF false ACCEPT Reactome (TAS) annotation of sphingosine N-acyltransferase (ceramide synthase) activity, the core MF. reaction_mf_exact accepted_mf local_module_mf|multi_gene_reactome_id 3 sphingolipid_de_novo_synthesis:GO:0050291 sphingosine N-acyltransferase activity human CERS3 genes/human/CERS3/CERS3-ai-review.yaml CERS3 Q8IU89 NCBITaxon:9606 Homo sapiens 19 R-HSA-428185 Ceramide synthases transfer acyl-CoA onto sphingoid yes reactome/R-HSA-428185.md Ceramide synthases transfer acyl-CoA onto sphingoid Homo sapiens GO:0050291 sphingosine N-acyltransferase activity TAS enables MF false ACCEPT Reactome (TAS) annotation of the core ceramide synthase / sphingosine N-acyltransferase activity, redundant with the experimental and IBA annotations. reaction_mf_exact accepted_mf local_module_mf|multi_gene_reactome_id 3 sphingolipid_de_novo_synthesis:GO:0050291 sphingosine N-acyltransferase activity human CFTR genes/human/CFTR/CFTR-ai-review.yaml CFTR P13569 NCBITaxon:9606 Homo sapiens 102 R-HSA-383190 HCO3- transport through ion channel yes reactome/R-HSA-383190.md HCO3- transport through ion channel Homo sapiens GO:0017081 chloride channel regulator activity TAS enables MF false ACCEPT This TAS annotation from Reactome suggests CFTR has chloride channel regulator activity, which is accurate as CFTR regulates other chloride channels like ORCC and ClC channels. reaction_mf_exact accepted_mf 1 human CFTR genes/human/CFTR/CFTR-ai-review.yaml CFTR P13569 NCBITaxon:9606 Homo sapiens 105 R-HSA-5678822 Defective CFTR does not transport Cl- from cytosol to extracellular region yes reactome/R-HSA-5678822.md Defective CFTR does not transport Cl- from cytosol to extracellular region Homo sapiens GO:0015108 chloride transmembrane transporter activity TAS enables MF false ACCEPT This TAS annotation from Reactome correctly identifies CFTR's chloride transport function. reaction_mf_exact accepted_mf reactome_disease_event 1 human CFTR genes/human/CFTR/CFTR-ai-review.yaml CFTR P13569 NCBITaxon:9606 Homo sapiens 106 R-HSA-5678863 CFTR transports Cl- from cytosol to extracellular region yes reactome/R-HSA-5678863.md CFTR transports Cl- from cytosol to extracellular region Homo sapiens GO:0015108 chloride transmembrane transporter activity TAS enables MF false ACCEPT This TAS annotation from Reactome correctly identifies CFTR's chloride transport function. reaction_mf_exact accepted_mf 1 human CHEK1 genes/human/CHEK1/CHEK1-ai-review.yaml CHEK1 O14757 NCBITaxon:9606 Homo sapiens 14 R-HSA-5685230 CHEK1 phosphorylates RAD51 yes reactome/R-HSA-5685230.md CHEK1 phosphorylates RAD51 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Protein serine/threonine kinase activity (Reactome TAS) for CHK1 checkpoint phosphorylation events. reaction_mf_exact accepted_mf 1 human CHEK1 genes/human/CHEK1/CHEK1-ai-review.yaml CHEK1 O14757 NCBITaxon:9606 Homo sapiens 15 R-HSA-5685242 CHEK1 phosphorylates BRCA2 yes reactome/R-HSA-5685242.md CHEK1 phosphorylates BRCA2 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Protein serine/threonine kinase activity (Reactome TAS) for CHK1 checkpoint phosphorylation events. reaction_mf_exact accepted_mf 1 human CHEK1 genes/human/CHEK1/CHEK1-ai-review.yaml CHEK1 O14757 NCBITaxon:9606 Homo sapiens 16 R-HSA-6799246 CHEK1 phosphorylates TP53 yes reactome/R-HSA-6799246.md CHEK1 phosphorylates TP53 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Protein serine/threonine kinase activity (Reactome TAS) for CHK1 checkpoint phosphorylation events. reaction_mf_exact accepted_mf 1 human CHEK1 genes/human/CHEK1/CHEK1-ai-review.yaml CHEK1 O14757 NCBITaxon:9606 Homo sapiens 17 R-HSA-69604 Phosphorylation of CDC25A by CHEK1 yes reactome/R-HSA-69604.md Phosphorylation of CDC25A by CHEK1 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Protein serine/threonine kinase activity (Reactome TAS) for CHK1 checkpoint phosphorylation events. reaction_mf_exact accepted_mf 1 human CHEK1 genes/human/CHEK1/CHEK1-ai-review.yaml CHEK1 O14757 NCBITaxon:9606 Homo sapiens 18 R-HSA-75010 Phosphorylation of Cdc25C at Ser 216 by Chk1 yes reactome/R-HSA-75010.md Phosphorylation of Cdc25C at Ser 216 by Chk1 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Protein serine/threonine kinase activity (Reactome TAS) for CHK1 checkpoint phosphorylation events. reaction_mf_exact accepted_mf 1 human CHEK1 genes/human/CHEK1/CHEK1-ai-review.yaml CHEK1 O14757 NCBITaxon:9606 Homo sapiens 19 R-HSA-9007539 CHEK1 phosphorylates E2F6 yes reactome/R-HSA-9007539.md CHEK1 phosphorylates E2F6 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Protein serine/threonine kinase activity (Reactome TAS) for CHK1 checkpoint phosphorylation events. reaction_mf_exact accepted_mf 1 human CHEK1 genes/human/CHEK1/CHEK1-ai-review.yaml CHEK1 O14757 NCBITaxon:9606 Homo sapiens 20 R-HSA-9943662 CHEK1 phosphorylates NEK11 yes reactome/R-HSA-9943662.md CHEK1 phosphorylates NEK11 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Protein serine/threonine kinase activity (Reactome TAS) for CHK1 checkpoint phosphorylation events. reaction_mf_exact accepted_mf 1 human CHEK1 genes/human/CHEK1/CHEK1-ai-review.yaml CHEK1 O14757 NCBITaxon:9606 Homo sapiens 21 R-HSA-9943678 CHEK1 phosphorylates CHEK2-phosphorylated CDC25A yes reactome/R-HSA-9943678.md CHEK1 phosphorylates CHEK2-phosphorylated CDC25A Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Protein serine/threonine kinase activity (Reactome TAS) for CHK1 checkpoint phosphorylation events. reaction_mf_exact accepted_mf 1 human COASY genes/human/COASY/COASY-ai-review.yaml COASY Q13057 NCBITaxon:9606 Homo sapiens 15 R-HSA-196773 COASY phosphorylates DP-CoA yes reactome/R-HSA-196773.md COASY phosphorylates DP-CoA Homo sapiens GO:0004140 dephospho-CoA kinase activity TAS enables MF false ACCEPT "Reactome reaction ""COASY phosphorylates DP-CoA"" captures the DPCK activity by authoritative pathway curation. Consistent with the experimental evidence. Core molecular function." reaction_mf_exact accepted_mf 1 human COASY genes/human/COASY/COASY-ai-review.yaml COASY Q13057 NCBITaxon:9606 Homo sapiens 16 R-HSA-196754 COASY transfers an adenylyl group from ATP to PPANT yes reactome/R-HSA-196754.md COASY transfers an adenylyl group from ATP to PPANT Homo sapiens GO:0004595 pantetheine-phosphate adenylyltransferase activity TAS enables MF false ACCEPT "Reactome reaction ""COASY transfers an adenylyl group from ATP to PPANT"" captures the PPAT activity by authoritative pathway curation. Consistent with the experimental evidence. Core molecular function." reaction_mf_exact accepted_mf 1 human COLGALT1 genes/human/COLGALT1/COLGALT1-ai-review.yaml COLGALT1 Q8NBJ5 NCBITaxon:9606 Homo sapiens 5 R-HSA-1981120 Galactosylation of collagen propeptide hydroxylysines by procollagen galactosyltransferases 1, 2 yes reactome/R-HSA-1981120.md Galactosylation of collagen propeptide hydroxylysines by procollagen galactosyltransferases 1, 2 Homo sapiens GO:0050211 procollagen galactosyltransferase activity TAS enables MF false ACCEPT Reactome-asserted procollagen galactosyltransferase activity, the core molecular function of COLGALT1. reaction_mf_exact accepted_mf multi_gene_reactome_id 2 human COLGALT2 genes/human/COLGALT2/COLGALT2-ai-review.yaml COLGALT2 Q8IYK4 NCBITaxon:9606 Homo sapiens 9 R-HSA-1981120 Galactosylation of collagen propeptide hydroxylysines by procollagen galactosyltransferases 1, 2 yes reactome/R-HSA-1981120.md Galactosylation of collagen propeptide hydroxylysines by procollagen galactosyltransferases 1, 2 Homo sapiens GO:0050211 procollagen galactosyltransferase activity TAS enables MF false ACCEPT Core molecular function captured from Reactome (galactosylation of collagen propeptide hydroxylysines). Concordant with the direct biochemical evidence. reaction_mf_exact accepted_mf multi_gene_reactome_id 2 human COP1 genes/human/COP1/COP1-ai-review.yaml COP1 Q8NHY2 NCBITaxon:9606 Homo sapiens 18 R-HSA-264444 Autoubiquitination of phospho-COP1(Ser-387 ) yes reactome/R-HSA-264444.md Autoubiquitination of phospho-COP1(Ser-387 ) Homo sapiens GO:0004842 ubiquitin-protein transferase activity TAS enables MF false ACCEPT TAS (Reactome) assignment of ubiquitin-protein transferase activity, capturing COP1 autoubiquitination. This is a correct molecular-function term for COP1's E3 ligase activity (a parent/sibling of GO:0061630). reaction_mf_exact accepted_mf 1 human COQ2 genes/human/COQ2/COQ2-ai-review.yaml COQ2 Q96H96 NCBITaxon:9606 Homo sapiens 12 R-HSA-2162192 COQ2 ligates all-E-10PrP2 to PHB yes reactome/R-HSA-2162192.md COQ2 ligates all-E-10PrP2 to PHB Homo sapiens GO:0008412 4-hydroxybenzoate polyprenyltransferase activity TAS enables MF false ACCEPT Reactome traceable-author assignment of the defining molecular function, describing the reaction in which COQ2 ligates all-trans-decaprenyl diphosphate to 4-hydroxybenzoate to form 3-decaprenyl-4-hydroxybenzoate. Correct and core. reaction_mf_exact accepted_mf 1 human COQ4 genes/human/COQ4/COQ4-ai-review.yaml COQ4 Q9Y3A0 NCBITaxon:9606 Homo sapiens 13 R-HSA-2162195 COQ4 decarboxylates MHDB yes reactome/R-HSA-2162195.md COQ4 decarboxylates MHDB Homo sapiens GO:0120539 4-hydroxy-3-methoxy-5-polyprenylbenzoate decarboxylase activity TAS enables MF false ACCEPT "Reactome reaction ""COQ4 decarboxylates MHDB"" assigns the C1 decarboxylase activity to COQ4. Consistent with the experimentally established core molecular function." reaction_mf_exact accepted_mf 1 human COQ5 genes/human/COQ5/COQ5-ai-review.yaml COQ5 Q5HYK3 NCBITaxon:9606 Homo sapiens 10 R-HSA-2162188 COQ5 methylates MDMQ10H2 yes reactome/R-HSA-2162188.md COQ5 methylates MDMQ10H2 Homo sapiens GO:0008425 2-methoxy-6-polyprenyl-1,4-benzoquinol methyltransferase activity TAS enables MF false ACCEPT "Correct core MF asserted by Reactome for the COQ5-catalyzed methylation reaction (R-HSA-2162188 ""COQ5 methylates MDMQ10H2""); consistent with the experimental IDA annotations." reaction_mf_exact accepted_mf 1 human COX10 genes/human/COX10/COX10-ai-review.yaml COX10 Q12887 NCBITaxon:9606 Homo sapiens 16 R-HSA-2995330 COX10 transforms heme to heme O yes reactome/R-HSA-2995330.md COX10 transforms heme to heme O Homo sapiens GO:0008495 protoheme IX farnesyltransferase activity TAS enables MF false ACCEPT COX10 converts protoheme IX/heme b and farnesyl diphosphate to heme O. reaction_mf_exact accepted_mf 1 human COX15 genes/human/COX15/COX15-ai-review.yaml COX15 Q7KZN9 NCBITaxon:9606 Homo sapiens 11 R-HSA-2995334 COX15 transforms heme O to heme A yes reactome/R-HSA-2995334.md COX15 transforms heme O to heme A Homo sapiens GO:0120547 heme A synthase activity TAS enables MF false ACCEPT COX15 catalyzes conversion of heme O to heme A, the specific heme A synthase reaction. reaction_mf_exact accepted_mf 1 human CSNK1D genes/human/CSNK1D/CSNK1D-ai-review.yaml CSNK1D P48730 NCBITaxon:9606 Homo sapiens 34 R-HSA-5694441 CSNK1D phosphorylates SEC23 yes reactome/R-HSA-5694441.md CSNK1D phosphorylates SEC23 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Reactome (TAS) annotation of protein serine/threonine kinase activity in the context of SEC23 phosphorylation. Core MF. reaction_mf_exact accepted_mf 1 human CUBN genes/human/CUBN/CUBN-ai-review.yaml CUBN O60494 NCBITaxon:9606 Homo sapiens 14 R-HSA-350186 CUBN binds GC:25(OH)D yes reactome/R-HSA-350186.md CUBN binds GC:25(OH)D Homo sapiens GO:0038024 cargo receptor activity TAS enables MF false ACCEPT "Reactome TAS annotation for cargo receptor activity (""CUBN binds GC:25(OH)D""). This is the core molecular function of cubilin - a non-enzymatic multiligand cargo/endocytic receptor." reaction_mf_exact accepted_mf 1 human CYB5R4 genes/human/CYB5R4/CYB5R4-ai-review.yaml CYB5R4 Q7L1T6 NCBITaxon:9606 Homo sapiens 12 R-HSA-6806831 CYB5Rs reduce MetHb to HbA yes reactome/R-HSA-6806831.md CYB5Rs reduce MetHb to HbA Homo sapiens GO:0004128 cytochrome-b5 reductase activity, acting on NAD(P)H TAS enables MF false ACCEPT Reactome TAS annotation of the core cytochrome-b5 reductase activity (in the context of methemoglobin reduction), consistent with experimental data. reaction_mf_exact accepted_mf 1 human CYP11A1 genes/human/CYP11A1/CYP11A1-ai-review.yaml CYP11A1 P05108 NCBITaxon:9606 Homo sapiens 25 R-HSA-193054 Oxidation of cholesterol to 22beta-hydroxycholesterol yes reactome/R-HSA-193054.md Oxidation of cholesterol to 22beta-hydroxycholesterol Homo sapiens GO:0008386 cholesterol monooxygenase (side-chain-cleaving) activity TAS enables MF false ACCEPT Reactome TAS annotation for the first hydroxylation step (cholesterol to 22beta-hydroxycholesterol) catalyzed by CYP11A1, supporting the core cholesterol side-chain-cleaving activity. reaction_mf_exact accepted_mf 1 human CYP11A1 genes/human/CYP11A1/CYP11A1-ai-review.yaml CYP11A1 P05108 NCBITaxon:9606 Homo sapiens 26 R-HSA-193065 Oxidation of 22beta-hydroxycholesterol to 20alpha,22beta-hydroxycholesterol yes reactome/R-HSA-193065.md Oxidation of 22beta-hydroxycholesterol to 20alpha,22beta-hydroxycholesterol Homo sapiens GO:0008386 cholesterol monooxygenase (side-chain-cleaving) activity TAS enables MF false ACCEPT Reactome TAS annotation for the second hydroxylation step (22beta-hydroxycholesterol to 20alpha,22beta-hydroxycholesterol) catalyzed by CYP11A1. reaction_mf_exact accepted_mf 1 human CYP11A1 genes/human/CYP11A1/CYP11A1-ai-review.yaml CYP11A1 P05108 NCBITaxon:9606 Homo sapiens 27 R-HSA-193101 CYP11A1 cleaves 20a,22b-DHCHOL yes reactome/R-HSA-193101.md CYP11A1 cleaves 20a,22b-DHCHOL Homo sapiens GO:0008386 cholesterol monooxygenase (side-chain-cleaving) activity TAS enables MF false ACCEPT Reactome TAS annotation for the final C20-C22 bond cleavage step (20alpha,22beta-dihydroxycholesterol to pregnenolone) catalyzed by CYP11A1. reaction_mf_exact accepted_mf local_module_mf 1 adrenal_steroidogenesis:GO:0008386 cholesterol monooxygenase (side-chain-cleaving) activity human CYP11B1 genes/human/CYP11B1/CYP11B1-ai-review.yaml CYP11B1 P15538 NCBITaxon:9606 Homo sapiens 21 R-HSA-193997 CYP11B1 oxidises 11DCORT yes reactome/R-HSA-193997.md CYP11B1 oxidises 11DCORT Homo sapiens GO:0004507 steroid 11-beta-monooxygenase activity TAS enables MF false ACCEPT "Reactome (TAS) annotation from the reaction ""CYP11B1 oxidises 11DCORT"" (11-deoxycortisol to cortisol), the core catalytic activity." reaction_mf_exact accepted_mf local_module_mf 1 adrenal_steroidogenesis:GO:0004507 steroid 11-beta-monooxygenase activity human CYP11B1 genes/human/CYP11B1/CYP11B1-ai-review.yaml CYP11B1 P15538 NCBITaxon:9606 Homo sapiens 22 R-HSA-5580292 Defective CYP11B1 does not oxidise 11DCORT yes reactome/R-HSA-5580292.md Defective CYP11B1 does not oxidise 11DCORT Homo sapiens GO:0004507 steroid 11-beta-monooxygenase activity TAS enables MF false ACCEPT "Reactome (TAS) annotation from the disease reaction ""Defective CYP11B1 does not oxidise 11DCORT"", capturing the same 11beta-hydroxylase molecular function in the context of AH4." reaction_mf_exact accepted_mf reactome_disease_event 1 human CYP11B2 genes/human/CYP11B2/CYP11B2-ai-review.yaml CYP11B2 P19099 NCBITaxon:9606 Homo sapiens 21 R-HSA-194017 CYP11B2 oxidises 11DCORST to CORST yes reactome/R-HSA-194017.md CYP11B2 oxidises 11DCORST to CORST Homo sapiens GO:0004507 steroid 11-beta-monooxygenase activity TAS enables MF false ACCEPT "Reactome TAS for the reaction ""CYP11B2 oxidises 11DCORST to CORST"" - 11-beta-hydroxylation of 11-deoxycorticosterone to corticosterone." reaction_mf_exact accepted_mf 1 human CYP11B2 genes/human/CYP11B2/CYP11B2-ai-review.yaml CYP11B2 P19099 NCBITaxon:9606 Homo sapiens 22 R-HSA-5600598 Defective CYP11B2 does not oxidise 11DCORST yes reactome/R-HSA-5600598.md Defective CYP11B2 does not oxidise 11DCORST Homo sapiens GO:0004507 steroid 11-beta-monooxygenase activity TAS enables MF false ACCEPT "Reactome TAS (disease reaction ""Defective CYP11B2 does not oxidise 11DCORST"") annotating the normal 11-beta-monooxygenase activity of CYP11B2." reaction_mf_exact accepted_mf reactome_disease_event 1 human CYP11B2 genes/human/CYP11B2/CYP11B2-ai-review.yaml CYP11B2 P19099 NCBITaxon:9606 Homo sapiens 31 R-HSA-193965 CYP11B2 oxidises 18HCORST to ALDO yes reactome/R-HSA-193965.md CYP11B2 oxidises 18HCORST to ALDO Homo sapiens GO:0008395 steroid hydroxylase activity TAS enables MF false ACCEPT Reactome TAS grouping CYP11B2's activity under steroid hydroxylase activity. Correct parent term encompassing its 11-beta- and 18-hydroxylase activities. reaction_mf_exact accepted_mf local_module_mf 1 adrenal_steroidogenesis:GO:0047783 corticosterone 18-monooxygenase activity human CYP11B2 genes/human/CYP11B2/CYP11B2-ai-review.yaml CYP11B2 P19099 NCBITaxon:9606 Homo sapiens 32 R-HSA-193995 CYP11B2 18-hydroxylates CORST to 18HCORST yes reactome/R-HSA-193995.md CYP11B2 18-hydroxylates CORST to 18HCORST Homo sapiens GO:0008395 steroid hydroxylase activity TAS enables MF false ACCEPT Reactome TAS grouping CYP11B2 under steroid hydroxylase activity (from the 18-hydroxylation reaction). Correct parent term. reaction_mf_exact accepted_mf local_module_mf 1 adrenal_steroidogenesis:GO:0047783 corticosterone 18-monooxygenase activity human CYP11B2 genes/human/CYP11B2/CYP11B2-ai-review.yaml CYP11B2 P19099 NCBITaxon:9606 Homo sapiens 33 R-HSA-6785244 Defective CYP11B2 does not oxidise 18HCORST yes reactome/R-HSA-6785244.md Defective CYP11B2 does not oxidise 18HCORST Homo sapiens GO:0008395 steroid hydroxylase activity TAS enables MF false ACCEPT Reactome TAS (disease reaction) grouping CYP11B2 under steroid hydroxylase activity. reaction_mf_exact accepted_mf reactome_disease_event 1 human CYP11B2 genes/human/CYP11B2/CYP11B2-ai-review.yaml CYP11B2 P19099 NCBITaxon:9606 Homo sapiens 34 R-HSA-6785245 Defective CYP11B2 does not oxidise CORST yes reactome/R-HSA-6785245.md Defective CYP11B2 does not oxidise CORST Homo sapiens GO:0008395 steroid hydroxylase activity TAS enables MF false ACCEPT Reactome TAS (disease reaction) grouping CYP11B2 under steroid hydroxylase activity. reaction_mf_exact accepted_mf reactome_disease_event 1 human CYP17A1 genes/human/CYP17A1/CYP17A1-ai-review.yaml CYP17A1 P05093 NCBITaxon:9606 Homo sapiens 19 R-HSA-193068 CYP17A1 17-hydroxylates PREG yes reactome/R-HSA-193068.md CYP17A1 17-hydroxylates PREG Homo sapiens GO:0004508 steroid 17-alpha-monooxygenase activity TAS enables MF false ACCEPT "Reactome reaction ""CYP17A1 17-hydroxylates PREG"" annotates the core steroid 17-alpha-monooxygenase activity." reaction_mf_exact accepted_mf 1 human CYP17A1 genes/human/CYP17A1/CYP17A1-ai-review.yaml CYP17A1 P05093 NCBITaxon:9606 Homo sapiens 20 R-HSA-193070 CYP17A1 cleaves 17aHPREG to DHA yes reactome/R-HSA-193070.md CYP17A1 cleaves 17aHPREG to DHA Homo sapiens GO:0004508 steroid 17-alpha-monooxygenase activity TAS enables MF false ACCEPT "Reactome reaction ""CYP17A1 cleaves 17aHPREG to DHA"" annotates the core steroid 17-alpha-monooxygenase/lyase activity (DHEA production)." reaction_mf_exact accepted_mf 1 human CYP17A1 genes/human/CYP17A1/CYP17A1-ai-review.yaml CYP17A1 P05093 NCBITaxon:9606 Homo sapiens 21 R-HSA-193072 CYP17A1 17-hydroxylates P4 to 17aHPROG yes reactome/R-HSA-193072.md CYP17A1 17-hydroxylates P4 to 17aHPROG Homo sapiens GO:0004508 steroid 17-alpha-monooxygenase activity TAS enables MF false ACCEPT "Reactome reaction ""CYP17A1 17-hydroxylates P4 to 17aHPROG"" annotates the core steroid 17-alpha-monooxygenase activity (progesterone hydroxylation)." reaction_mf_exact accepted_mf local_module_mf 1 adrenal_steroidogenesis:GO:0004508 steroid 17-alpha-monooxygenase activity human CYP17A1 genes/human/CYP17A1/CYP17A1-ai-review.yaml CYP17A1 P05093 NCBITaxon:9606 Homo sapiens 22 R-HSA-193099 CYP17A1 cleaves 17aHPROG to ANDST yes reactome/R-HSA-193099.md CYP17A1 cleaves 17aHPROG to ANDST Homo sapiens GO:0004508 steroid 17-alpha-monooxygenase activity TAS enables MF false ACCEPT "Reactome reaction ""CYP17A1 cleaves 17aHPROG to ANDST"" annotates the core steroid 17-alpha-monooxygenase/lyase activity (androstenedione production)." reaction_mf_exact accepted_mf local_module_mf 1 adrenal_steroidogenesis:GO:0004508 steroid 17-alpha-monooxygenase activity human CYP17A1 genes/human/CYP17A1/CYP17A1-ai-review.yaml CYP17A1 P05093 NCBITaxon:9606 Homo sapiens 23 R-HSA-5601843 Defective CYP17A1 does not 17-hydroxylate PREG yes reactome/R-HSA-5601843.md Defective CYP17A1 does not 17-hydroxylate PREG Homo sapiens GO:0004508 steroid 17-alpha-monooxygenase activity TAS enables MF false ACCEPT Reactome reaction (defective CYP17A1 does not 17-hydroxylate PREG) annotates the core steroid 17-alpha-monooxygenase activity in the context of loss-of-function disease. reaction_mf_exact accepted_mf reactome_disease_event 1 human CYP17A1 genes/human/CYP17A1/CYP17A1-ai-review.yaml CYP17A1 P05093 NCBITaxon:9606 Homo sapiens 24 R-HSA-9035954 Defective CYP17A1 does not 17-hydroxylate P4 yes reactome/R-HSA-9035954.md Defective CYP17A1 does not 17-hydroxylate P4 Homo sapiens GO:0004508 steroid 17-alpha-monooxygenase activity TAS enables MF false ACCEPT Reactome reaction (defective CYP17A1 does not 17-hydroxylate P4) annotates the core steroid 17-alpha-monooxygenase activity in the loss-of-function disease context. reaction_mf_exact accepted_mf reactome_disease_event 1 human CYP17A1 genes/human/CYP17A1/CYP17A1-ai-review.yaml CYP17A1 P05093 NCBITaxon:9606 Homo sapiens 25 R-HSA-9035956 Defective CYP17A1 does not cleave 17aHPROG yes reactome/R-HSA-9035956.md Defective CYP17A1 does not cleave 17aHPROG Homo sapiens GO:0004508 steroid 17-alpha-monooxygenase activity TAS enables MF false ACCEPT Reactome reaction (defective CYP17A1 does not cleave 17aHPROG) annotates the core steroid 17-alpha-monooxygenase/lyase activity in the loss-of-function disease context. reaction_mf_exact accepted_mf reactome_disease_event 1 human CYP21A2 genes/human/CYP21A2/CYP21A2-ai-review.yaml CYP21A2 P08686 NCBITaxon:9606 Homo sapiens 20 R-HSA-5601976 Defective CYP21A2 does not 21-hydroxylate PROG yes reactome/R-HSA-5601976.md Defective CYP21A2 does not 21-hydroxylate PROG Homo sapiens GO:0004509 steroid 21-monooxygenase activity TAS enables MF false ACCEPT Reactome (TAS) assignment of the core catalytic activity, in the context of defective CYP21A2 failing to 21-hydroxylate progesterone. Correct core molecular function. reaction_mf_exact accepted_mf reactome_disease_event 1 human CYP21A2 genes/human/CYP21A2/CYP21A2-ai-review.yaml CYP21A2 P08686 NCBITaxon:9606 Homo sapiens 21 R-HSA-193964 CYP21A2 21-hydroxylates PROG yes reactome/R-HSA-193964.md CYP21A2 21-hydroxylates PROG Homo sapiens GO:0106309 progesterone 21-hydroxylase activity TAS enables MF false ACCEPT Reactome (TAS) assignment of the specific progesterone 21-hydroxylase reaction (CYP21A2 21-hydroxylates PROG). Correct and appropriately specific. reaction_mf_exact accepted_mf local_module_mf 1 adrenal_steroidogenesis:GO:0004509 steroid 21-monooxygenase activity human CYP21A2 genes/human/CYP21A2/CYP21A2-ai-review.yaml CYP21A2 P08686 NCBITaxon:9606 Homo sapiens 22 R-HSA-193981 CYP21A2 oxidises 17HPROG yes reactome/R-HSA-193981.md CYP21A2 oxidises 17HPROG Homo sapiens GO:0106309 progesterone 21-hydroxylase activity TAS enables MF false ACCEPT Reactome (TAS) assignment of progesterone 21-hydroxylase activity (duplicate of the reaction annotation via a different Reactome event). Correct and appropriately specific. reaction_mf_exact accepted_mf local_module_mf 1 adrenal_steroidogenesis:GO:0004509 steroid 21-monooxygenase activity human CYP27A1 genes/human/CYP27A1/CYP27A1-ai-review.yaml CYP27A1 Q02318 NCBITaxon:9606 Homo sapiens 31 R-HSA-191999 CYP27A1 27-hydroxylates 5bCHOL3a,7a,12a-triol yes reactome/R-HSA-191999.md CYP27A1 27-hydroxylates 5bCHOL3a,7a,12a-triol Homo sapiens GO:0008395 steroid hydroxylase activity TAS enables MF false ACCEPT Reactome TAS (CYP27A1 27-hydroxylates 5b-cholestane-3a,7a,12a-triol). Correct; a general parent of the specific sterol 26/27-hydroxylase activity. Accept. reaction_mf_exact accepted_mf local_module_mf 1 bile_acid_synthesis_sidechain_conjugation:GO:0047748 cholestanetetraol 26-dehydrogenase activity human CYP27A1 genes/human/CYP27A1/CYP27A1-ai-review.yaml CYP27A1 Q02318 NCBITaxon:9606 Homo sapiens 32 R-HSA-192042 5beta-cholestan-3alpha,7alpha,12alpha,27-tetrol is oxidized to 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-27-al yes reactome/R-HSA-192042.md 5beta-cholestan-3alpha,7alpha,12alpha,27-tetrol is oxidized to 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-27-al Homo sapiens GO:0008395 steroid hydroxylase activity TAS enables MF false ACCEPT Reactome TAS for a CYP27A1 side-chain oxidation step in bile-acid biosynthesis; steroid hydroxylase is a correct general parent MF. Accept (duplicate). reaction_mf_exact accepted_mf 1 human CYP27A1 genes/human/CYP27A1/CYP27A1-ai-review.yaml CYP27A1 Q02318 NCBITaxon:9606 Homo sapiens 33 R-HSA-192054 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-27-al is oxidized to 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoate (THCA) yes reactome/R-HSA-192054.md 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-27-al is oxidized to 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoate (THCA) Homo sapiens GO:0008395 steroid hydroxylase activity TAS enables MF false ACCEPT Reactome TAS for a CYP27A1 bile-acid side-chain oxidation step; steroid hydroxylase is a correct general parent MF. Accept (duplicate). reaction_mf_exact accepted_mf 1 human CYP27A1 genes/human/CYP27A1/CYP27A1-ai-review.yaml CYP27A1 Q02318 NCBITaxon:9606 Homo sapiens 34 R-HSA-192123 CYP27A1 27-hydroxylates CHOL yes reactome/R-HSA-192123.md CYP27A1 27-hydroxylates CHOL Homo sapiens GO:0008395 steroid hydroxylase activity TAS enables MF false ACCEPT Reactome TAS (CYP27A1 27-hydroxylates cholesterol); steroid hydroxylase is a correct general parent MF. Accept (duplicate). reaction_mf_exact accepted_mf 1 human CYP27A1 genes/human/CYP27A1/CYP27A1-ai-review.yaml CYP27A1 Q02318 NCBITaxon:9606 Homo sapiens 35 R-HSA-193393 5beta-cholestan-3alpha, 7alpha-diol is hydroxylated to 5beta-cholestan-3alpha, 7alpha, 26-triol yes reactome/R-HSA-193393.md 5beta-cholestan-3alpha, 7alpha-diol is hydroxylated to 5beta-cholestan-3alpha, 7alpha, 26-triol Homo sapiens GO:0008395 steroid hydroxylase activity TAS enables MF false ACCEPT Reactome TAS for a CYP27A1 sterol-hydroxylation step; correct general parent MF. Accept (duplicate). reaction_mf_exact accepted_mf 1 human CYP27A1 genes/human/CYP27A1/CYP27A1-ai-review.yaml CYP27A1 Q02318 NCBITaxon:9606 Homo sapiens 36 R-HSA-193460 3alpha, 7alpha-dihydroxy-5beta-cholestan-26-al is oxidized to 3alpha, 7alpha-dihydroxy-5beta-cholestanoate (DHCA) yes reactome/R-HSA-193460.md 3alpha, 7alpha-dihydroxy-5beta-cholestan-26-al is oxidized to 3alpha, 7alpha-dihydroxy-5beta-cholestanoate (DHCA) Homo sapiens GO:0008395 steroid hydroxylase activity TAS enables MF false ACCEPT Reactome TAS for a CYP27A1 side-chain oxidation step; correct general parent MF. Accept (duplicate). reaction_mf_exact accepted_mf 1 human CYP27A1 genes/human/CYP27A1/CYP27A1-ai-review.yaml CYP27A1 Q02318 NCBITaxon:9606 Homo sapiens 37 R-HSA-193497 5beta-cholestan-3alpha, 7alpha, 26-triol is oxidized to 3alpha, 7alpha-dihydroxy-5beta-cholestan-26-al yes reactome/R-HSA-193497.md 5beta-cholestan-3alpha, 7alpha, 26-triol is oxidized to 3alpha, 7alpha-dihydroxy-5beta-cholestan-26-al Homo sapiens GO:0008395 steroid hydroxylase activity TAS enables MF false ACCEPT Reactome TAS for a CYP27A1 sterol-hydroxylation step; correct general parent MF. Accept (duplicate). reaction_mf_exact accepted_mf 1 human CYP27A1 genes/human/CYP27A1/CYP27A1-ai-review.yaml CYP27A1 Q02318 NCBITaxon:9606 Homo sapiens 38 R-HSA-193713 TODO: Fetch title no GO:0008395 steroid hydroxylase activity TAS enables MF false ACCEPT Reactome TAS for a CYP27A1 sterol-hydroxylation step; correct general parent MF. Accept (duplicate). reaction_mf_exact accepted_mf uncached_reactome|missing_reactome_title 1 human CYP27A1 genes/human/CYP27A1/CYP27A1-ai-review.yaml CYP27A1 Q02318 NCBITaxon:9606 Homo sapiens 39 R-HSA-193719 5beta-cholestan-3alpha,7alpha,24(S),27-tetrol is oxidized to 3alpha,7alpha,24(S)-trihydroxy-5beta-cholestan-27-al yes reactome/R-HSA-193719.md 5beta-cholestan-3alpha,7alpha,24(S),27-tetrol is oxidized to 3alpha,7alpha,24(S)-trihydroxy-5beta-cholestan-27-al Homo sapiens GO:0008395 steroid hydroxylase activity TAS enables MF false ACCEPT Reactome TAS for a CYP27A1 sterol-hydroxylation step; correct general parent MF. Accept (duplicate). reaction_mf_exact accepted_mf 1 human CYP27A1 genes/human/CYP27A1/CYP27A1-ai-review.yaml CYP27A1 Q02318 NCBITaxon:9606 Homo sapiens 40 R-HSA-193737 3alpha,7alpha,24(S)-trihydroxy-5beta-cholestan-27-al is oxidized to 3alpha,7alpha,24(S)-trihydroxy-5beta-cholestanoate (3,7,24THCA) yes reactome/R-HSA-193737.md 3alpha,7alpha,24(S)-trihydroxy-5beta-cholestan-27-al is oxidized to 3alpha,7alpha,24(S)-trihydroxy-5beta-cholestanoate (3,7,24THCA) Homo sapiens GO:0008395 steroid hydroxylase activity TAS enables MF false ACCEPT Reactome TAS for a CYP27A1 side-chain oxidation step; correct general parent MF. Accept (duplicate). reaction_mf_exact accepted_mf 1 human CYP27A1 genes/human/CYP27A1/CYP27A1-ai-review.yaml CYP27A1 Q02318 NCBITaxon:9606 Homo sapiens 41 R-HSA-193780 TODO: Fetch title no GO:0008395 steroid hydroxylase activity TAS enables MF false ACCEPT Reactome TAS for a CYP27A1 sterol-hydroxylation step; correct general parent MF. Accept (duplicate). reaction_mf_exact accepted_mf uncached_reactome|missing_reactome_title 1 human CYP27A1 genes/human/CYP27A1/CYP27A1-ai-review.yaml CYP27A1 Q02318 NCBITaxon:9606 Homo sapiens 42 R-HSA-193787 TODO: Fetch title no GO:0008395 steroid hydroxylase activity TAS enables MF false ACCEPT Reactome TAS for a CYP27A1 sterol-hydroxylation step; correct general parent MF. Accept (duplicate). reaction_mf_exact accepted_mf uncached_reactome|missing_reactome_title 1 human CYP27A1 genes/human/CYP27A1/CYP27A1-ai-review.yaml CYP27A1 Q02318 NCBITaxon:9606 Homo sapiens 43 R-HSA-193792 CYP27A1 27-hydroxylates 5β-CHOL3α,7α,24(s)-triol yes reactome/R-HSA-193792.md CYP27A1 27-hydroxylates 5β-CHOL3α,7α,24(s)-triol Homo sapiens GO:0008395 steroid hydroxylase activity TAS enables MF false ACCEPT Reactome TAS (CYP27A1 27-hydroxylates 5b-CHOL3a,7a,24(s)-triol); correct general parent MF. Accept (duplicate). reaction_mf_exact accepted_mf 1 human CYP27A1 genes/human/CYP27A1/CYP27A1-ai-review.yaml CYP27A1 Q02318 NCBITaxon:9606 Homo sapiens 44 R-HSA-5602170 CYP27A1 does not 27-hydroxylate 5bCHOL3a,7a,12a-triol yes reactome/R-HSA-5602170.md CYP27A1 does not 27-hydroxylate 5bCHOL3a,7a,12a-triol Homo sapiens GO:0008395 steroid hydroxylase activity TAS enables MF false ACCEPT Reactome TAS from a 'CYP27A1 does not 27-hydroxylate ...' reaction record, but annotated as enabling steroid hydroxylase activity. The general MF is correct for CYP27A1; accept (duplicate). reaction_mf_exact accepted_mf 1 human CYP27A1 genes/human/CYP27A1/CYP27A1-ai-review.yaml CYP27A1 Q02318 NCBITaxon:9606 Homo sapiens 45 R-HSA-9035960 Defective CYP27A1 does not 27-hydroxylate 5β-CHOL3α,7α,24(s)-triol yes reactome/R-HSA-9035960.md Defective CYP27A1 does not 27-hydroxylate 5β-CHOL3α,7α,24(s)-triol Homo sapiens GO:0008395 steroid hydroxylase activity TAS enables MF false ACCEPT Reactome TAS (defective CYP27A1 reaction record) annotated as enabling steroid hydroxylase activity; the general MF is correct for wild-type CYP27A1. Accept (duplicate). reaction_mf_exact accepted_mf reactome_disease_event 1 human CYP39A1 genes/human/CYP39A1/CYP39A1-ai-review.yaml CYP39A1 Q9NYL5 NCBITaxon:9606 Homo sapiens 19 R-HSA-192178 CYP39A1 7-hydroxylates 24OH-CHOL yes reactome/R-HSA-192178.md CYP39A1 7-hydroxylates 24OH-CHOL Homo sapiens GO:0008396 oxysterol 7-alpha-hydroxylase activity TAS enables MF false ACCEPT "Correct. UniProt lists ""Oxysterol 7-alpha-hydroxylase"" as an alternative name and CYP39A1 was cloned as such. This is a valid MF; however, CYP39A1 is specifically selective for 24-hydroxycholesterol, so the more precise GO:0033782 better captures the substrate specificity. Retained." reaction_mf_exact accepted_mf 1 human CYP46A1 genes/human/CYP46A1/CYP46A1-ai-review.yaml CYP46A1 Q9Y6A2 NCBITaxon:9606 Homo sapiens 21 R-HSA-192061 CYP46A1 24-hydroxylates CHOL yes reactome/R-HSA-192061.md CYP46A1 24-hydroxylates CHOL Homo sapiens GO:0033781 cholesterol 24-hydroxylase activity TAS enables MF false ACCEPT Reactome (TAS) assertion of the core catalytic activity (CYP46A1 24-hydroxylates cholesterol). Redundant with experimental annotations; the defining molecular function. reaction_mf_exact accepted_mf 1 human CYP7A1 genes/human/CYP7A1/CYP7A1-ai-review.yaml CYP7A1 P22680 NCBITaxon:9606 Homo sapiens 25 R-HSA-192051 CYP7A1 7-hydroxylates CHOL yes reactome/R-HSA-192051.md CYP7A1 7-hydroxylates CHOL Homo sapiens GO:0008123 cholesterol 7-alpha-monooxygenase activity TAS enables MF false ACCEPT Core molecular function, curated by Reactome as the CYP7A1 7-hydroxylation of cholesterol reaction. reaction_mf_exact accepted_mf local_module_mf 1 bile_acid_synthesis_ring_modification:GO:0008123 cholesterol 7-alpha-monooxygenase activity human CYP7B1 genes/human/CYP7B1/CYP7B1-ai-review.yaml CYP7B1 O75881 NCBITaxon:9606 Homo sapiens 19 R-HSA-191972 27-hydroxycholesterol is 7alpha-hydroxylated yes reactome/R-HSA-191972.md 27-hydroxycholesterol is 7alpha-hydroxylated Homo sapiens GO:0008396 oxysterol 7-alpha-hydroxylase activity TAS enables MF false ACCEPT Reactome TAS (27-hydroxycholesterol is 7alpha-hydroxylated) for oxysterol 7-alpha-hydroxylase activity. This is the specific, core molecular function of CYP7B1. reaction_mf_exact accepted_mf 1 human CYP7B1 genes/human/CYP7B1/CYP7B1-ai-review.yaml CYP7B1 O75881 NCBITaxon:9606 Homo sapiens 20 R-HSA-192065 CYP7B1 7-hydroxylates 25OH-CHOL yes reactome/R-HSA-192065.md CYP7B1 7-hydroxylates 25OH-CHOL Homo sapiens GO:0008396 oxysterol 7-alpha-hydroxylase activity TAS enables MF false ACCEPT Reactome TAS (CYP7B1 7-hydroxylates 25OH-CHOL) for oxysterol 7-alpha-hydroxylase activity. Specific, core MF; consistent with the experimental 25-hydroxycholesterol activity. reaction_mf_exact accepted_mf local_module_mf 1 bile_acid_synthesis_ring_modification:GO:0008396 oxysterol 7-alpha-hydroxylase activity human CYP7B1 genes/human/CYP7B1/CYP7B1-ai-review.yaml CYP7B1 O75881 NCBITaxon:9606 Homo sapiens 21 R-HSA-5602885 Defective CYP7B1 does not 7-hydroxylate 25OH-CHOL yes reactome/R-HSA-5602885.md Defective CYP7B1 does not 7-hydroxylate 25OH-CHOL Homo sapiens GO:0008396 oxysterol 7-alpha-hydroxylase activity TAS enables MF false ACCEPT Reactome TAS (Defective CYP7B1 does not 7-hydroxylate 25OH-CHOL) for oxysterol 7-alpha-hydroxylase activity, framed around the disease (SPG5A/CBAS3) loss of activity. Correct specific core MF. reaction_mf_exact accepted_mf reactome_disease_event 1 human CYP8B1 genes/human/CYP8B1/CYP8B1-ai-review.yaml CYP8B1 Q9UNU6 NCBITaxon:9606 Homo sapiens 17 R-HSA-192157 CYP8B1 12-hydroxylates 4CHOL7aOLONE yes reactome/R-HSA-192157.md CYP8B1 12-hydroxylates 4CHOL7aOLONE Homo sapiens GO:0008397 sterol 12-alpha-hydroxylase activity TAS enables MF false ACCEPT Reactome TAS for the defining reaction (CYP8B1 12-hydroxylates 7-alpha-hydroxycholest-4-en-3-one). Correct core molecular function. Accept. reaction_mf_exact accepted_mf 1 human CYP8B1 genes/human/CYP8B1/CYP8B1-ai-review.yaml CYP8B1 Q9UNU6 NCBITaxon:9606 Homo sapiens 18 R-HSA-193709 TODO: Fetch title no GO:0008397 sterol 12-alpha-hydroxylase activity TAS enables MF false ACCEPT Reactome TAS for the sterol 12-alpha-hydroxylase reaction. Correct core molecular function; redundant Reactome support. Accept. reaction_mf_exact accepted_mf uncached_reactome|missing_reactome_title 1 human CYP8B1 genes/human/CYP8B1/CYP8B1-ai-review.yaml CYP8B1 Q9UNU6 NCBITaxon:9606 Homo sapiens 19 R-HSA-193845 CYP8B1 12-hydroxylates 4CHOL7a,27DONE yes reactome/R-HSA-193845.md CYP8B1 12-hydroxylates 4CHOL7a,27DONE Homo sapiens GO:0008397 sterol 12-alpha-hydroxylase activity TAS enables MF false ACCEPT Reactome TAS for the defining reaction (CYP8B1 12-hydroxylates 7-alpha,27-dihydroxy intermediate). Correct core molecular function. Accept. reaction_mf_exact accepted_mf 1 human DEGS1 genes/human/DEGS1/DEGS1-ai-review.yaml DEGS1 O15121 NCBITaxon:9606 Homo sapiens 14 R-HSA-428259 DEGS1 dehydrogenates dihydroceramide yes reactome/R-HSA-428259.md DEGS1 dehydrogenates dihydroceramide Homo sapiens GO:0042284 sphingolipid delta-4 desaturase activity TAS enables MF false ACCEPT Reactome traceable assertion of the core desaturase reaction (DEGS1 dehydrogenates dihydroceramide). Correct core molecular function. reaction_mf_exact accepted_mf local_module_mf 1 sphingolipid_de_novo_synthesis:GO:0042284 sphingolipid delta-4 desaturase activity human DHCR24 genes/human/DHCR24/DHCR24-ai-review.yaml DHCR24 Q15392 NCBITaxon:9606 Homo sapiens 33 R-HSA-9755937 DHCR24 reduces LAN to 24,25-dhLAN no GO:0050614 Delta24-sterol reductase activity TAS enables MF false ACCEPT TAS annotation from Reactome for Delta24-sterol reductase activity, specifically for lanosterol reduction. This is a validated core molecular function of DHCR24. reaction_mf_exact accepted_mf uncached_reactome 1 human DHRS3 genes/human/DHRS3/DHRS3-ai-review.yaml DHRS3 O75911 NCBITaxon:9606 Homo sapiens 9 R-HSA-2465940 atRAL is reduced to atROL yes reactome/R-HSA-2465940.md atRAL is reduced to atROL Homo sapiens GO:0052650 all-trans-retinol dehydrogenase (NADP+) activity TAS enables MF false ACCEPT Core molecular function, curated by Reactome (atRAL is reduced to atROL using NADP+/NADPH). Consistent with UniProt EC 1.1.1.300. Accept. reaction_mf_exact accepted_mf 1 human DHRS3 genes/human/DHRS3/DHRS3-ai-review.yaml DHRS3 O75911 NCBITaxon:9606 Homo sapiens 10 R-HSA-5419165 RDH11,14,DHRS3,DRHS4 reduce atRAL to atROL yes reactome/R-HSA-5419165.md RDH11,14,DHRS3,DRHS4 reduce atRAL to atROL Homo sapiens GO:0052650 all-trans-retinol dehydrogenase (NADP+) activity TAS enables MF false ACCEPT Core molecular function, curated by Reactome (DHRS3 among reductases that reduce atRAL to atROL). Consistent with the NADP+ reaction in UniProt. Accept. reaction_mf_exact accepted_mf multi_gene_reactome_id 2 human DLAT genes/human/DLAT/DLAT-ai-review.yaml DLAT P10515 NCBITaxon:9606 Homo sapiens 20 R-HSA-9861667 DLAT trimer transfers acetyl to CoA no GO:0004742 dihydrolipoyllysine-residue acetyltransferase activity TAS enables MF false ACCEPT Reactome TAS for the DLAT-catalyzed transfer of the acetyl group from acetyllipoyl-lysine to CoA. This is the defining E2 reaction. reaction_mf_exact accepted_mf uncached_reactome 1 human DNAJB1 genes/human/DNAJB1/DNAJB1-ai-review.yaml DNAJB1 P25685 NCBITaxon:9606 Homo sapiens 41 R-HSA-5251955 HSP40s activate intrinsic ATPase activity of HSP70s in the nucleoplasm yes reactome/R-HSA-5251955.md HSP40s activate intrinsic ATPase activity of HSP70s in the nucleoplasm Homo sapiens GO:0001671 ATPase activator activity TAS enables MF false ACCEPT Reactome R-HSA-5251955 models HSP40s activating intrinsic ATPase activity of HSP70s in the nucleoplasm. DNAJB1 translocates to the nucleus under heat shock and activates HSP70 ATPase activity there. reaction_mf_exact accepted_mf multi_gene_reactome_id 3 human DNAJB1 genes/human/DNAJB1/DNAJB1-ai-review.yaml DNAJB1 P25685 NCBITaxon:9606 Homo sapiens 42 R-HSA-5251959 HSP40s activate intrinsic ATPase activity of HSP70s in the cytosol yes reactome/R-HSA-5251959.md HSP40s activate intrinsic ATPase activity of HSP70s in the cytosol Homo sapiens GO:0001671 ATPase activator activity TAS enables MF false ACCEPT Reactome R-HSA-5251959 models HSP40s activating intrinsic ATPase activity of HSP70s in the cytosol. DNAJB1 is explicitly listed as one of the HSP40s that modulates intrinsic ATPase activity of HSP70s. reaction_mf_exact accepted_mf multi_gene_reactome_id 3 human DNAJB6 genes/human/DNAJB6/DNAJB6-ai-review.yaml DNAJB6 O75190 NCBITaxon:9606 Homo sapiens 25 R-HSA-5251955 HSP40s activate intrinsic ATPase activity of HSP70s in the nucleoplasm yes reactome/R-HSA-5251955.md HSP40s activate intrinsic ATPase activity of HSP70s in the nucleoplasm Homo sapiens GO:0001671 ATPase activator activity TAS enables MF false ACCEPT This TAS annotation from Reactome (R-HSA-5251955, HSP40s activate intrinsic ATPase activity of HSP70s in the nucleoplasm) correctly identifies DNAJB6 as an activator of HSP70 ATPase activity. The Reactome pathway summary explicitly lists DNAJB6 (as DNAJB6) among the HSP40s that stimulate HSP70 ATPase activity, citing Izawa et al. 2000 (PMID:10954706) and Hanai & Mashima 2003. This is a core molecular function of all J-domain proteins. reaction_mf_exact accepted_mf multi_gene_reactome_id 3 human DNAJB6 genes/human/DNAJB6/DNAJB6-ai-review.yaml DNAJB6 O75190 NCBITaxon:9606 Homo sapiens 26 R-HSA-5251959 HSP40s activate intrinsic ATPase activity of HSP70s in the cytosol yes reactome/R-HSA-5251959.md HSP40s activate intrinsic ATPase activity of HSP70s in the cytosol Homo sapiens GO:0001671 ATPase activator activity TAS enables MF false ACCEPT This TAS annotation from Reactome (R-HSA-5251959, HSP40s activate intrinsic ATPase activity of HSP70s in the cytosol) correctly identifies DNAJB6 as an activator of HSP70 ATPase activity in the cytosol. The Reactome pathway explicitly mentions DNAJB6 (as DNAJB6) among the HSP40s that dramatically increase HSP70 ATPase activity, citing Izawa et al. 2000 (PMID:10954706). Duplicate of the nucleoplasm-specific annotation above but refers to the cytosolic context (isoform B). reaction_mf_exact accepted_mf multi_gene_reactome_id 3 human DNAJC7 genes/human/DNAJC7/DNAJC7-ai-review.yaml DNAJC7 Q99615 NCBITaxon:9606 Homo sapiens 17 R-HSA-5251955 HSP70/HSP90 chaperone cycle (Reactome) yes reactome/R-HSA-5251955.md HSP40s activate intrinsic ATPase activity of HSP70s in the nucleoplasm Homo sapiens GO:0001671 ATPase activator activity TAS enables MF false ACCEPT DNAJC7's J domain stimulates ATP hydrolysis by HSP70, the defining molecular function of J-domain co-chaperones. Directly demonstrated biochemically. reaction_mf_exact accepted_mf multi_gene_reactome_id 3 human DNAJC7 genes/human/DNAJC7/DNAJC7-ai-review.yaml DNAJC7 Q99615 NCBITaxon:9606 Homo sapiens 18 R-HSA-5251959 HSP70/HSP90 chaperone cycle (Reactome) yes reactome/R-HSA-5251959.md HSP40s activate intrinsic ATPase activity of HSP70s in the cytosol Homo sapiens GO:0001671 ATPase activator activity TAS enables MF false ACCEPT Duplicate Reactome-sourced annotation of HSP70 ATPase stimulation by the J domain, a core molecular function of DNAJC7. reaction_mf_exact accepted_mf multi_gene_reactome_id 3 human DOLK genes/human/DOLK/DOLK-ai-review.yaml DOLK Q9UPQ8 NCBITaxon:9606 Homo sapiens 12 R-HSA-446195 DOLK phosphorylates DCHOL to DOLP yes reactome/R-HSA-446195.md DOLK phosphorylates DCHOL to DOLP Homo sapiens GO:0004168 dolichol kinase activity TAS enables MF false ACCEPT Reactome-asserted dolichol kinase activity (DOLK phosphorylates dolichol to Dol-P), matching the core molecular function. reaction_mf_exact accepted_mf local_module_mf 1 dolichol_phosphate_sugar_donor_supply:GO:0004168 dolichol kinase activity human DOLK genes/human/DOLK/DOLK-ai-review.yaml DOLK Q9UPQ8 NCBITaxon:9606 Homo sapiens 13 R-HSA-4755600 Defective DOLK does not phosphorylate DCHOL yes reactome/R-HSA-4755600.md Defective DOLK does not phosphorylate DCHOL Homo sapiens GO:0004168 dolichol kinase activity TAS enables MF false ACCEPT Reactome-asserted dolichol kinase activity (disease-annotated reaction), matching the core molecular function. reaction_mf_exact accepted_mf reactome_disease_event 1 human DOLPP1 genes/human/DOLPP1/DOLPP1-ai-review.yaml DOLPP1 Q86YN1 NCBITaxon:9606 Homo sapiens 8 R-HSA-446200 DOLPP1 dephosphorylates DOLDP to DOLP yes reactome/R-HSA-446200.md DOLPP1 dephosphorylates DOLDP to DOLP Homo sapiens GO:0047874 dolichyldiphosphatase activity TAS enables MF false ACCEPT "Correct core molecular function, curated by Reactome (reaction ""DOLPP1 dephosphorylates DOLDP to DOLP""). Consistent with the UniProt catalytic activity and EC 3.6.1.43." reaction_mf_exact accepted_mf 1 human DPAGT1 genes/human/DPAGT1/DPAGT1-ai-review.yaml DPAGT1 Q9H3H5 NCBITaxon:9606 Homo sapiens 10 R-HSA-446191 Addition of N-acetyl-D-glucosamine to Dolichyl phosphate yes reactome/R-HSA-446191.md Addition of N-acetyl-D-glucosamine to Dolichyl phosphate Homo sapiens GO:0003975 UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity TAS enables MF false ACCEPT Reactome TAS for the GlcNAc-1-P transferase reaction (addition of N-acetylglucosamine to dolichyl phosphate). Matches the defining catalytic activity of DPAGT1. reaction_mf_exact accepted_mf local_module_mf 1 n_glycan_llo_assembly_cytoplasmic:GO:0003975 UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity human DPAGT1 genes/human/DPAGT1/DPAGT1-ai-review.yaml DPAGT1 Q9H3H5 NCBITaxon:9606 Homo sapiens 11 R-HSA-4549334 Defective DPAGT1 does not transfer GlcNAc to DOLP yes reactome/R-HSA-4549334.md Defective DPAGT1 does not transfer GlcNAc to DOLP Homo sapiens GO:0003975 UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity TAS enables MF false ACCEPT Reactome TAS (disease-variant reaction 'Defective DPAGT1 does not transfer GlcNAc to DOLP') again attributing the GlcNAc-1-P transferase activity to DPAGT1. Duplicate of the core catalytic function. reaction_mf_exact accepted_mf reactome_disease_event 1 human DUOX1 genes/human/DUOX1/DUOX1-ai-review.yaml DUOX1 Q9NRD9 NCBITaxon:9606 Homo sapiens 29 R-HSA-5693681 DUOX1 reduces O2 to H2O2 yes reactome/R-HSA-5693681.md DUOX1 reduces O2 to H2O2 Homo sapiens GO:0016174 NAD(P)H oxidase H2O2-forming activity TAS enables MF false ACCEPT NAD(P)H oxidase H2O2-forming activity: Human DUOX1–DUOXA1 structures and functional experiments establish a membrane oxidase complex using heme, FAD and NADPH to generate extracellular H2O2, regulated by calcium-binding EF-hand elements. These are direct components of the oxidase mechanism. reaction_mf_exact accepted_mf 1 human ECHS1 genes/human/ECHS1/ECHS1-ai-review.yaml ECHS1 P30084 NCBITaxon:9606 Homo sapiens 25 R-HSA-77256 2-trans-Dodecenoyl-CoA+H2O =>( S)-3-Hydroxydodecanoyl-CoA yes reactome/R-HSA-77256.md 2-trans-Dodecenoyl-CoA+H2O =>( S)-3-Hydroxydodecanoyl-CoA Homo sapiens GO:0004300 enoyl-CoA hydratase activity TAS enables MF false ACCEPT Reactome (TAS) annotation of enoyl-CoA hydratase activity for a specific beta-oxidation reaction (2-trans-dodecenoyl-CoA hydration). reaction_mf_exact accepted_mf 1 human ECHS1 genes/human/ECHS1/ECHS1-ai-review.yaml ECHS1 P30084 NCBITaxon:9606 Homo sapiens 26 R-HSA-77314 Crotonoyl-CoA+H2O => (S)-3-Hydroxybutanoyl-CoA yes reactome/R-HSA-77314.md Crotonoyl-CoA+H2O => (S)-3-Hydroxybutanoyl-CoA Homo sapiens GO:0004300 enoyl-CoA hydratase activity TAS enables MF false ACCEPT Reactome (TAS) enoyl-CoA hydratase activity for crotonoyl-CoA hydration. reaction_mf_exact accepted_mf 1 human ECHS1 genes/human/ECHS1/ECHS1-ai-review.yaml ECHS1 P30084 NCBITaxon:9606 Homo sapiens 27 R-HSA-77325 trans-Hex-2-enoyl-CoA+H2O<=>(S)-Hydroxyhexanoyl-CoA yes reactome/R-HSA-77325.md trans-Hex-2-enoyl-CoA+H2O<=>(S)-Hydroxyhexanoyl-CoA Homo sapiens GO:0004300 enoyl-CoA hydratase activity TAS enables MF false ACCEPT Reactome (TAS) enoyl-CoA hydratase activity for trans-hex-2-enoyl-CoA hydration. reaction_mf_exact accepted_mf 1 human ECHS1 genes/human/ECHS1/ECHS1-ai-review.yaml ECHS1 P30084 NCBITaxon:9606 Homo sapiens 28 R-HSA-77333 trans-Oct-2-enoyl-CoA+H2O => (S)-Hydroxyoctanoyl-CoA yes reactome/R-HSA-77333.md trans-Oct-2-enoyl-CoA+H2O => (S)-Hydroxyoctanoyl-CoA Homo sapiens GO:0004300 enoyl-CoA hydratase activity TAS enables MF false ACCEPT Reactome (TAS) enoyl-CoA hydratase activity for trans-oct-2-enoyl-CoA hydration. reaction_mf_exact accepted_mf 1 human ECHS1 genes/human/ECHS1/ECHS1-ai-review.yaml ECHS1 P30084 NCBITaxon:9606 Homo sapiens 29 R-HSA-77344 trans-Dec-2-enoyl-CoA+H2O => (S)-Hydroxydecanoyl-CoA yes reactome/R-HSA-77344.md trans-Dec-2-enoyl-CoA+H2O => (S)-Hydroxydecanoyl-CoA Homo sapiens GO:0004300 enoyl-CoA hydratase activity TAS enables MF false ACCEPT Reactome (TAS) enoyl-CoA hydratase activity for trans-dec-2-enoyl-CoA hydration. reaction_mf_exact accepted_mf 1 human ECHS1 genes/human/ECHS1/ECHS1-ai-review.yaml ECHS1 P30084 NCBITaxon:9606 Homo sapiens 30 R-HSA-9916717 ECHS1 mutants don't synthesize beta-hydroxyisobutyryl-CoA yes reactome/R-HSA-9916717.md ECHS1 mutants don't synthesize beta-hydroxyisobutyryl-CoA Homo sapiens GO:0004300 enoyl-CoA hydratase activity TAS enables MF false ACCEPT Reactome (TAS) enoyl-CoA hydratase activity within the ECHS1-deficiency disease pathway (synthesis of beta-hydroxyisobutyryl-CoA from methacrylyl-CoA in valine catabolism). reaction_mf_exact accepted_mf 1 human EDEM1 genes/human/EDEM1/EDEM1-ai-review.yaml EDEM1 Q92611 NCBITaxon:9606 Homo sapiens 9 R-HSA-6782685 EDEM1,3 hydrolyse (GlcNAc)2 (Man)8b to (GlcNAc)2 (Man)5 yes reactome/R-HSA-6782685.md EDEM1,3 hydrolyse (GlcNAc)2 (Man)8b to (GlcNAc)2 (Man)5 Homo sapiens GO:0004571 mannosyl-oligosaccharide 1,2-alpha-mannosidase activity TAS enables MF false ACCEPT Reactome curation of EDEM1 (with EDEM3) hydrolysing Man8 to Man5 glycans. EDEM1 has genuine but low alpha-1,2-mannosidase activity contributing to the second trimming step. reaction_mf_exact accepted_mf multi_gene_reactome_id 2 human EDEM2 genes/human/EDEM2/EDEM2-ai-review.yaml EDEM2 Q9BV94 NCBITaxon:9606 Homo sapiens 10 R-HSA-901024 MAN1B1 hydrolyses 1,2-linked mannose (a branch) yes reactome/R-HSA-901024.md MAN1B1 hydrolyses 1,2-linked mannose (a branch) Homo sapiens GO:0004571 mannosyl-oligosaccharide 1,2-alpha-mannosidase activity TAS enables MF false ACCEPT Reactome curation of EDEM2 alpha-1,2-mannosidase activity in N-glycan mannose trimming. EDEM2 has demonstrated mannosidase activity catalyzing the first trimming step (Man9 to Man8B). reaction_mf_exact accepted_mf multi_gene_reactome_id 2 human EDEM2 genes/human/EDEM2/EDEM2-ai-review.yaml EDEM2 Q9BV94 NCBITaxon:9606 Homo sapiens 11 R-HSA-901036 MAN1B1 hydrolyses a second 1,2-linked mannose (a branch) yes reactome/R-HSA-901036.md MAN1B1 hydrolyses a second 1,2-linked mannose (a branch) Homo sapiens GO:0004571 mannosyl-oligosaccharide 1,2-alpha-mannosidase activity TAS enables MF false ACCEPT Reactome curation of EDEM2 alpha-1,2-mannosidase activity in N-glycan mannose trimming. EDEM2 has demonstrated mannosidase activity catalyzing the first trimming step (Man9 to Man8B). reaction_mf_exact accepted_mf multi_gene_reactome_id 2 human EDEM2 genes/human/EDEM2/EDEM2-ai-review.yaml EDEM2 Q9BV94 NCBITaxon:9606 Homo sapiens 12 R-HSA-901039 MAN1B1 hydrolyses 1,2-linked mannose (c branch) yes reactome/R-HSA-901039.md MAN1B1 hydrolyses 1,2-linked mannose (c branch) Homo sapiens GO:0004571 mannosyl-oligosaccharide 1,2-alpha-mannosidase activity TAS enables MF false ACCEPT Reactome curation of EDEM2 alpha-1,2-mannosidase activity in N-glycan mannose trimming. EDEM2 has demonstrated mannosidase activity catalyzing the first trimming step (Man9 to Man8B). reaction_mf_exact accepted_mf multi_gene_reactome_id 2 human EDEM2 genes/human/EDEM2/EDEM2-ai-review.yaml EDEM2 Q9BV94 NCBITaxon:9606 Homo sapiens 13 R-HSA-901074 MAN1B1,EDEM2 hydrolyse 1,2-linked mannose (b branch) yes reactome/R-HSA-901074.md MAN1B1,EDEM2 hydrolyse 1,2-linked mannose (b branch) Homo sapiens GO:0004571 mannosyl-oligosaccharide 1,2-alpha-mannosidase activity TAS enables MF false ACCEPT Reactome curation of EDEM2 alpha-1,2-mannosidase activity in N-glycan mannose trimming. EDEM2 has demonstrated mannosidase activity catalyzing the first trimming step (Man9 to Man8B). reaction_mf_exact accepted_mf multi_gene_reactome_id 2 human EDEM2 genes/human/EDEM2/EDEM2-ai-review.yaml EDEM2 Q9BV94 NCBITaxon:9606 Homo sapiens 14 R-HSA-9696807 N-glycan mannose trimming of Spike yes reactome/R-HSA-9696807.md N-glycan mannose trimming of Spike Homo sapiens GO:0004571 mannosyl-oligosaccharide 1,2-alpha-mannosidase activity TAS enables MF false ACCEPT Reactome curation of EDEM2 alpha-1,2-mannosidase activity in N-glycan mannose trimming. EDEM2 has demonstrated mannosidase activity catalyzing the first trimming step (Man9 to Man8B). reaction_mf_exact accepted_mf multi_gene_reactome_id 2 human EDEM3 genes/human/EDEM3/EDEM3-ai-review.yaml EDEM3 Q9BZQ6 NCBITaxon:9606 Homo sapiens 13 R-HSA-6782685 EDEM1,3 hydrolyse (GlcNAc)2 (Man)8b to (GlcNAc)2 (Man)5 yes reactome/R-HSA-6782685.md EDEM1,3 hydrolyse (GlcNAc)2 (Man)8b to (GlcNAc)2 (Man)5 Homo sapiens GO:0004571 mannosyl-oligosaccharide 1,2-alpha-mannosidase activity TAS enables MF false ACCEPT Reactome curation of EDEM3 (with EDEM1) hydrolysing Man8b to Man5 glycans, an accurate representation of EDEM3's downstream trimming activity. reaction_mf_exact accepted_mf multi_gene_reactome_id 2 human EGFR genes/human/EGFR/EGFR-ai-review.yaml EGFR P00533 NCBITaxon:9606 Homo sapiens 221 R-HSA-177930 GAB1 phosphorylation by EGFR kinase yes reactome/R-HSA-177930.md GAB1 phosphorylation by EGFR kinase Homo sapiens GO:0004714 transmembrane receptor protein tyrosine kinase activity TAS enables MF false ACCEPT EGFR is a receptor tyrosine kinase (EC 2.7.10.1) that phosphorylates tyrosine residues on substrate proteins following ligand-induced dimerization. This is a core molecular function. reaction_mf_exact accepted_mf 1 human EGFR genes/human/EGFR/EGFR-ai-review.yaml EGFR P00533 NCBITaxon:9606 Homo sapiens 222 R-HSA-177933 SHC1 phosphorylation by phosphorylated EGFR yes reactome/R-HSA-177933.md SHC1 phosphorylation by phosphorylated EGFR Homo sapiens GO:0004714 transmembrane receptor protein tyrosine kinase activity TAS enables MF false ACCEPT EGFR is a receptor tyrosine kinase (EC 2.7.10.1) that phosphorylates tyrosine residues on substrate proteins following ligand-induced dimerization. This is a core molecular function. reaction_mf_exact accepted_mf 1 human EGFR genes/human/EGFR/EGFR-ai-review.yaml EGFR P00533 NCBITaxon:9606 Homo sapiens 244 R-HSA-177934 EGFR autophosphorylation yes reactome/R-HSA-177934.md EGFR autophosphorylation Homo sapiens GO:0004713 protein tyrosine kinase activity TAS enables MF false ACCEPT EGFR possesses protein tyrosine kinase activity, phosphorylating itself and downstream substrates upon activation. reaction_mf_exact accepted_mf 1 human EGFR genes/human/EGFR/EGFR-ai-review.yaml EGFR P00533 NCBITaxon:9606 Homo sapiens 245 R-HSA-182969 Phosphorylation of CBL (EGFR:CBL) yes reactome/R-HSA-182969.md Phosphorylation of CBL (EGFR:CBL) Homo sapiens GO:0004713 protein tyrosine kinase activity TAS enables MF false ACCEPT EGFR possesses protein tyrosine kinase activity, phosphorylating itself and downstream substrates upon activation. reaction_mf_exact accepted_mf 1 human EGFR genes/human/EGFR/EGFR-ai-review.yaml EGFR P00533 NCBITaxon:9606 Homo sapiens 246 R-HSA-183058 Phosphorylation of CBL (EGFR:GRB2:CBL) yes reactome/R-HSA-183058.md Phosphorylation of CBL (EGFR:GRB2:CBL) Homo sapiens GO:0004713 protein tyrosine kinase activity TAS enables MF false ACCEPT EGFR possesses protein tyrosine kinase activity, phosphorylating itself and downstream substrates upon activation. reaction_mf_exact accepted_mf 1 human EGFR genes/human/EGFR/EGFR-ai-review.yaml EGFR P00533 NCBITaxon:9606 Homo sapiens 247 R-HSA-212710 EGFR activates PLC-gamma1 by phosphorylation yes reactome/R-HSA-212710.md EGFR activates PLC-gamma1 by phosphorylation Homo sapiens GO:0004713 protein tyrosine kinase activity TAS enables MF false ACCEPT EGFR possesses protein tyrosine kinase activity, phosphorylating itself and downstream substrates upon activation. reaction_mf_exact accepted_mf 1 human EGFR genes/human/EGFR/EGFR-ai-review.yaml EGFR P00533 NCBITaxon:9606 Homo sapiens 248 R-HSA-8867041 EGFR phosphorylates EPS15 yes reactome/R-HSA-8867041.md EGFR phosphorylates EPS15 Homo sapiens GO:0004713 protein tyrosine kinase activity TAS enables MF false ACCEPT EGFR possesses protein tyrosine kinase activity, phosphorylating itself and downstream substrates upon activation. reaction_mf_exact accepted_mf 1 human EHHADH genes/human/EHHADH/EHHADH-ai-review.yaml EHHADH Q08426 NCBITaxon:9606 Homo sapiens 26 R-HSA-6809264 EHHADH dehydrogenates 3-hydroxyhexacosanoyl-CoA yes reactome/R-HSA-6809264.md EHHADH dehydrogenates 3-hydroxyhexacosanoyl-CoA Homo sapiens GO:0003857 (3S)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity TAS enables MF false ACCEPT Reactome TAS for the core C-terminal dehydrogenase activity (dehydrogenation of 3-hydroxyhexacosanoyl-CoA); consistent with human IDA evidence. reaction_mf_exact accepted_mf 1 human ELOVL2 genes/human/ELOVL2/ELOVL2-ai-review.yaml ELOVL2 Q9NXB9 NCBITaxon:9606 Homo sapiens 22 R-HSA-2046083 Elongation of arachidonyl-CoA to docosatetraenoyl-CoA yes reactome/R-HSA-2046083.md Elongation of arachidonyl-CoA to docosatetraenoyl-CoA Homo sapiens GO:0009922 fatty acid elongase activity TAS enables MF false ACCEPT Reactome TAS for the elongase condensation reaction (elongation of arachidonyl-CoA). Correct core molecular function; redundant with the experimental annotations. reaction_mf_exact accepted_mf multi_gene_reactome_id 2 human ELOVL2 genes/human/ELOVL2/ELOVL2-ai-review.yaml ELOVL2 Q9NXB9 NCBITaxon:9606 Homo sapiens 23 R-HSA-2046090 Elongation of docosapentaenoyl-CoA to tetracosapentaenoyl-CoA yes reactome/R-HSA-2046090.md Elongation of docosapentaenoyl-CoA to tetracosapentaenoyl-CoA Homo sapiens GO:0009922 fatty acid elongase activity TAS enables MF false ACCEPT Reactome TAS for an ELOVL2 elongation reaction (docosapentaenoyl-CoA -> tetracosapentaenoyl-CoA). Correct core molecular function; redundant with the experimental annotations. reaction_mf_exact accepted_mf 1 human ELOVL2 genes/human/ELOVL2/ELOVL2-ai-review.yaml ELOVL2 Q9NXB9 NCBITaxon:9606 Homo sapiens 24 R-HSA-2046095 Elongation of docosatetraenoyl-CoA to tetracosatetraenoyl-CoA yes reactome/R-HSA-2046095.md Elongation of docosatetraenoyl-CoA to tetracosatetraenoyl-CoA Homo sapiens GO:0009922 fatty acid elongase activity TAS enables MF false ACCEPT Reactome TAS for an ELOVL2 elongation reaction (docosatetraenoyl-CoA -> tetracosatetraenoyl-CoA). Correct core molecular function; redundant with the experimental annotations. reaction_mf_exact accepted_mf 1 human ELOVL2 genes/human/ELOVL2/ELOVL2-ai-review.yaml ELOVL2 Q9NXB9 NCBITaxon:9606 Homo sapiens 25 R-HSA-2046100 Elongation of eicosapentaenoyl-CoA to docosapentaenoyl-CoA yes reactome/R-HSA-2046100.md Elongation of eicosapentaenoyl-CoA to docosapentaenoyl-CoA Homo sapiens GO:0009922 fatty acid elongase activity TAS enables MF false ACCEPT Reactome TAS for an ELOVL2 elongation reaction (eicosapentaenoyl-CoA -> docosapentaenoyl-CoA). Correct core molecular function; redundant with the experimental annotations. reaction_mf_exact accepted_mf multi_gene_reactome_id 2 human ELOVL3 genes/human/ELOVL3/ELOVL3-ai-review.yaml ELOVL3 Q9HB03 NCBITaxon:9606 Homo sapiens 24 R-HSA-548814 ELOVL3,6,7 elongate PALM-CoA and Mal-CoA to 3OOD-CoA yes reactome/R-HSA-548814.md ELOVL3,6,7 elongate PALM-CoA and Mal-CoA to 3OOD-CoA Homo sapiens GO:0009922 fatty acid elongase activity TAS enables MF false ACCEPT "Reactome reaction ""ELOVL3,6,7 elongate PALM-CoA and Mal-CoA to 3OOD-CoA"" supports the core elongase MF (palmitoyl-CoA + malonyl-CoA condensation). Accept." reaction_mf_exact accepted_mf multi_gene_reactome_id 3 human ELOVL5 genes/human/ELOVL5/ELOVL5-ai-review.yaml ELOVL5 Q9NYP7 NCBITaxon:9606 Homo sapiens 30 R-HSA-2046083 Elongation of arachidonyl-CoA to docosatetraenoyl-CoA yes reactome/R-HSA-2046083.md Elongation of arachidonyl-CoA to docosatetraenoyl-CoA Homo sapiens GO:0009922 fatty acid elongase activity TAS enables MF false ACCEPT Reactome reaction annotation for elongation of arachidonyl-CoA to docosatetraenoyl-CoA. reaction_mf_exact accepted_mf multi_gene_reactome_id 2 human ELOVL5 genes/human/ELOVL5/ELOVL5-ai-review.yaml ELOVL5 Q9NYP7 NCBITaxon:9606 Homo sapiens 31 R-HSA-2046100 Elongation of eicosapentaenoyl-CoA to docosapentaenoyl-CoA yes reactome/R-HSA-2046100.md Elongation of eicosapentaenoyl-CoA to docosapentaenoyl-CoA Homo sapiens GO:0009922 fatty acid elongase activity TAS enables MF false ACCEPT Reactome reaction annotation for elongation of eicosapentaenoyl-CoA to docosapentaenoyl-CoA. reaction_mf_exact accepted_mf multi_gene_reactome_id 2 human ELOVL6 genes/human/ELOVL6/ELOVL6-ai-review.yaml ELOVL6 Q9H5J4 NCBITaxon:9606 Homo sapiens 22 R-HSA-548814 ELOVL3,6,7 elongate PALM-CoA and Mal-CoA to 3OOD-CoA yes reactome/R-HSA-548814.md ELOVL3,6,7 elongate PALM-CoA and Mal-CoA to 3OOD-CoA Homo sapiens GO:0009922 fatty acid elongase activity TAS enables MF false ACCEPT Core molecular function, Reactome-traceable (ELOVL3,6,7 condense palmitoyl-CoA with malonyl-CoA to 3-oxooctadecanoyl-CoA). Correct. reaction_mf_exact accepted_mf multi_gene_reactome_id 3 human ELOVL7 genes/human/ELOVL7/ELOVL7-ai-review.yaml ELOVL7 A1L3X0 NCBITaxon:9606 Homo sapiens 24 R-HSA-548814 ELOVL3,6,7 elongate PALM-CoA and Mal-CoA to 3OOD-CoA yes reactome/R-HSA-548814.md ELOVL3,6,7 elongate PALM-CoA and Mal-CoA to 3OOD-CoA Homo sapiens GO:0009922 fatty acid elongase activity TAS enables MF false ACCEPT "TAS from Reactome reaction ""ELOVL3,6,7 elongate PALM-CoA and Mal-CoA to 3OOD-CoA"". Correctly asserts the core condensing (elongase) activity of ELOVL7 on palmitoyl-CoA + malonyl-CoA. Accept." reaction_mf_exact accepted_mf multi_gene_reactome_id 3 human ENO3 genes/human/ENO3/ENO3-ai-review.yaml ENO3 P13929 NCBITaxon:9606 Homo sapiens 16 R-HSA-70494 Enolase dimers (ENO1,2,3) convert PEP to 2PG yes reactome/R-HSA-70494.md Enolase dimers (ENO1,2,3) convert PEP to 2PG Homo sapiens GO:0004634 phosphopyruvate hydratase activity TAS enables MF false ACCEPT "Reactome traceable-author-statement for the enolase reaction (""Enolase dimers (ENO1,2,3) convert PEP to 2PG""), assigning phosphopyruvate hydratase activity to ENO3." reaction_mf_exact accepted_mf 1 human ENO3 genes/human/ENO3/ENO3-ai-review.yaml ENO3 P13929 NCBITaxon:9606 Homo sapiens 17 R-HSA-71660 Enolase dimers dehydrates 2PG yes reactome/R-HSA-71660.md Enolase dimers dehydrates 2PG Homo sapiens GO:0004634 phosphopyruvate hydratase activity TAS enables MF false ACCEPT "Reactome traceable-author-statement (""Enolase dimers dehydrates 2PG"") for the phosphopyruvate hydratase activity of ENO3." reaction_mf_exact accepted_mf local_module_mf 1 glycolysis_payoff_phase:GO:0004634 phosphopyruvate hydratase activity human ENPP7 genes/human/ENPP7/ENPP7-ai-review.yaml ENPP7 Q6UWV6 NCBITaxon:9606 Homo sapiens 9 R-HSA-1640164 ENPP7 hydrolyzes sphingomyelin yes reactome/R-HSA-1640164.md ENPP7 hydrolyzes sphingomyelin Homo sapiens GO:0004767 sphingomyelin phosphodiesterase activity TAS enables MF false ACCEPT Sphingomyelin phosphodiesterase activity from Reactome pathway - ENPP7 hydrolyzes sphingomyelin. reaction_mf_exact accepted_mf 1 human ESPL1 genes/human/ESPL1/ESPL1-ai-review.yaml ESPL1 Q14674 NCBITaxon:9606 Homo sapiens 31 R-HSA-2467775 Autocleavage of ESPL1 (Separase) yes reactome/R-HSA-2467775.md Autocleavage of ESPL1 (Separase) Homo sapiens GO:0008234 cysteine-type peptidase activity TAS enables MF false ACCEPT Reactome autocleavage reaction assigns cysteine-type peptidase activity to ESPL1. reaction_mf_exact accepted_mf 1 human ETFDH genes/human/ETFDH/ETFDH-ai-review.yaml ETFDH Q16134 NCBITaxon:9606 Homo sapiens 12 R-HSA-169270 ETFDH oxidises ETF (reduced) to ETF, reduces CoQ to CoQH2 no GO:0004174 electron-transferring-flavoprotein dehydrogenase activity TAS enables MF false ACCEPT Reactome reaction (ETFDH oxidises reduced ETF, reduces CoQ to CoQH2) assigning the core catalytic activity. Correct. reaction_mf_exact accepted_mf local_module_mf|uncached_reactome 1 electron_transfer_flavoprotein_system:GO:0004174 electron-transferring-flavoprotein dehydrogenase activity human FADS1 genes/human/FADS1/FADS1-ai-review.yaml FADS1 O60427 NCBITaxon:9606 Homo sapiens 20 R-HSA-2046089 Desaturation of eicosatetraenoyl-CoA to eicosapentaenoyl-CoA yes reactome/R-HSA-2046089.md Desaturation of eicosatetraenoyl-CoA to eicosapentaenoyl-CoA Homo sapiens GO:0045485 omega-6 fatty acid desaturase activity TAS enables MF false ACCEPT FADS1 acts on omega-6 fatty acid substrates (DGLA) to produce arachidonic acid. reaction_mf_exact accepted_mf 1 human FADS1 genes/human/FADS1/FADS1-ai-review.yaml FADS1 O60427 NCBITaxon:9606 Homo sapiens 21 R-HSA-2046092 Desaturation of dihomo-gamma-lenolenoyl-CoA to arachidonoyl-CoA yes reactome/R-HSA-2046092.md Desaturation of dihomo-gamma-lenolenoyl-CoA to arachidonoyl-CoA Homo sapiens GO:0045485 omega-6 fatty acid desaturase activity TAS enables MF false ACCEPT Duplicate annotation for omega-6 fatty acid desaturase activity from different Reactome reaction. reaction_mf_exact accepted_mf 1 human FEN1 genes/human/FEN1/FEN1-ai-review.yaml FEN1 P39748 NCBITaxon:9606 Homo sapiens 48 R-HSA-110363 FEN1 bound to PCNA and APEX1 cleaves flap ssDNA yes reactome/R-HSA-110363.md FEN1 bound to PCNA and APEX1 cleaves flap ssDNA Homo sapiens GO:0017108 5'-flap endonuclease activity TAS enables MF false ACCEPT Reactome TAS for 5'-flap endonuclease activity in the reaction 'FEN1 bound to PCNA and APEX1 cleaves flap ssDNA' (long-patch BER). reaction_mf_exact accepted_mf 1 human FEN1 genes/human/FEN1/FEN1-ai-review.yaml FEN1 P39748 NCBITaxon:9606 Homo sapiens 49 R-HSA-174446 Removal of remaining Flap from the C-strand yes reactome/R-HSA-174446.md Removal of remaining Flap from the C-strand Homo sapiens GO:0017108 5'-flap endonuclease activity TAS enables MF false ACCEPT Reactome TAS for 5'-flap endonuclease activity in 'Removal of remaining Flap from the C-strand' during telomere lagging-strand replication. reaction_mf_exact accepted_mf 1 human FEN1 genes/human/FEN1/FEN1-ai-review.yaml FEN1 P39748 NCBITaxon:9606 Homo sapiens 50 R-HSA-5651782 FEN1 bound to POLB cleaves displaced DNA strand (flap) yes reactome/R-HSA-5651782.md FEN1 bound to POLB cleaves displaced DNA strand (flap) Homo sapiens GO:0017108 5'-flap endonuclease activity TAS enables MF false ACCEPT Reactome TAS for 5'-flap endonuclease activity in 'FEN1 bound to POLB cleaves displaced DNA strand (flap)' during long-patch BER. reaction_mf_exact accepted_mf 1 human FEN1 genes/human/FEN1/FEN1-ai-review.yaml FEN1 P39748 NCBITaxon:9606 Homo sapiens 51 R-HSA-5687664 FEN1 cleaves displaced ssDNA flaps during MMEJ yes reactome/R-HSA-5687664.md FEN1 cleaves displaced ssDNA flaps during MMEJ Homo sapiens GO:0017108 5'-flap endonuclease activity TAS enables MF false ACCEPT Reactome TAS for 5'-flap endonuclease activity in 'FEN1 cleaves displaced ssDNA flaps during MMEJ'. reaction_mf_exact accepted_mf 1 human FEN1 genes/human/FEN1/FEN1-ai-review.yaml FEN1 P39748 NCBITaxon:9606 Homo sapiens 52 R-HSA-69152 Removal of remaining Flap yes reactome/R-HSA-69152.md Removal of remaining Flap Homo sapiens GO:0017108 5'-flap endonuclease activity TAS enables MF false ACCEPT Reactome TAS for 5'-flap endonuclease activity in 'Removal of remaining Flap' during Okazaki fragment processing. reaction_mf_exact accepted_mf 1 human FLAD1 genes/human/FLAD1/FLAD1-ai-review.yaml FLAD1 Q8NFF5 NCBITaxon:9606 Homo sapiens 20 R-HSA-196929 FLAD1 phosphorylates FMN yes reactome/R-HSA-196929.md FLAD1 phosphorylates FMN Homo sapiens GO:0003919 FMN adenylyltransferase activity TAS enables MF false ACCEPT Reactome traceable annotation of the core FMN adenylyltransferase reaction (FLAD1 adenylylates FMN). Correct core molecular function. reaction_mf_exact accepted_mf 1 human FXN genes/human/FXN/FXN-ai-review.yaml FXN Q16595 NCBITaxon:9606 Homo sapiens 31 R-HSA-1362416 Frataxin binds iron yes reactome/R-HSA-1362416.md Frataxin binds iron Homo sapiens GO:0008198 ferrous iron binding TAS enables MF false ACCEPT Reactome annotation for frataxin binding iron. reaction_mf_exact accepted_mf 1 human FXN genes/human/FXN/FXN-ai-review.yaml FXN Q16595 NCBITaxon:9606 Homo sapiens 44 R-HSA-9866272 2Fe-2S is inserted in UQCRFS1 yes reactome/R-HSA-9866272.md 2Fe-2S is inserted in UQCRFS1 Homo sapiens GO:0034986 iron chaperone activity TAS enables MF false ACCEPT Reactome annotation for iron chaperone function in cluster assembly. reaction_mf_exact accepted_mf local_module_mf 1 mitochondrial_complex_iii:GO:0008320 protein transmembrane transporter activity human G6PC1 genes/human/G6PC1/G6PC1-ai-review.yaml G6PC1 P35575 NCBITaxon:9606 Homo sapiens 22 R-HSA-3274540 Defective G6PC does not hydrolyze glucose 6-phosphate no GO:0004346 glucose-6-phosphatase activity TAS enables MF false ACCEPT Reactome traceable assignment of glucose-6-phosphatase activity (in the context of loss-of-function disease variants). Consistent with the core function. reaction_mf_exact accepted_mf reactome_disease_event|uncached_reactome 1 human G6PC3 genes/human/G6PC3/G6PC3-ai-review.yaml G6PC3 Q9BUM1 NCBITaxon:9606 Homo sapiens 12 R-HSA-3282876 Defective G6PC3 does not hydrolyze glucose 6-phosphate no GO:0004346 glucose-6-phosphatase activity TAS enables MF false ACCEPT Reactome traceable assignment of glucose-6-phosphatase activity (context: defective G6PC3 does not hydrolyze G6P), consistent with the core function. reaction_mf_exact accepted_mf reactome_disease_event|uncached_reactome 1 human GAL3ST1 genes/human/GAL3ST1/GAL3ST1-ai-review.yaml GAL3ST1 Q99999 NCBITaxon:9606 Homo sapiens 12 R-HSA-9844587 GAL3ST1 transfers sulfate to GalCer,LacCer yes reactome/R-HSA-9844587.md GAL3ST1 transfers sulfate to GalCer,LacCer Homo sapiens GO:0001733 galactosylceramide sulfotransferase activity TAS enables MF false ACCEPT Reactome reaction 'GAL3ST1 transfers sulfate to GalCer,LacCer'. reaction_mf_exact accepted_mf 1 human GALC genes/human/GALC/GALC-ai-review.yaml GALC P54803 NCBITaxon:9606 Homo sapiens 14 R-HSA-1606564 GALC hydrolyzes GalCer yes reactome/R-HSA-1606564.md GALC hydrolyzes GalCer Homo sapiens GO:0004336 galactosylceramidase activity TAS enables MF false ACCEPT "Reactome traceable assertion of galactosylceramidase activity (reaction ""GALC hydrolyzes GalCer""), matching the defining, experimentally verified core molecular function of GALC." reaction_mf_exact accepted_mf local_module_mf 1 glycosphingolipid_lysosomal_degradation:GO:0004336 galactosylceramidase activity human GALK1 genes/human/GALK1/GALK1-ai-review.yaml GALK1 P51570 NCBITaxon:9606 Homo sapiens 15 R-HSA-5610026 Defective GALK1 does not phosphorylate Gal no GO:0004335 galactokinase activity TAS enables MF false ACCEPT Reactome (TAS) assertion of galactokinase activity, corroborating the experimental annotations. reaction_mf_exact accepted_mf reactome_disease_event|uncached_reactome 1 human GALM genes/human/GALM/GALM-ai-review.yaml GALM Q96C23 NCBITaxon:9606 Homo sapiens 13 R-HSA-9931926 Defective GALM doesn't convert beta-D-GAL to alpha-D-GAL no GO:0004034 aldose 1-epimerase activity TAS enables MF false ACCEPT Reactome-curated (TAS) assertion of aldose 1-epimerase activity (in the context of the defective-GALM reaction). reaction_mf_exact accepted_mf reactome_disease_event|uncached_reactome 1 human GALNS genes/human/GALNS/GALNS-ai-review.yaml GALNS P34059 NCBITaxon:9606 Homo sapiens 4 R-HSA-2263490 Defective GALNS does not hydrolyse sulfate from Gal6S in keratan sulfate yes reactome/R-HSA-2263490.md Defective GALNS does not hydrolyse sulfate from Gal6S in keratan sulfate Homo sapiens GO:0043890 N-acetylgalactosamine-6-sulfatase activity TAS enables MF false ACCEPT Reactome-asserted core molecular function (6-sulfatase acting on Gal6S/GalNAc6S in keratan sulfate and chondroitin sulfate). reaction_mf_exact accepted_mf reactome_disease_event 1 human GALNT1 genes/human/GALNT1/GALNT1-ai-review.yaml GALNT1 Q10472 NCBITaxon:9606 Homo sapiens 12 R-HSA-913675 GALNTs transfer GalNAc to Mucins to form Tn antigens yes reactome/R-HSA-913675.md GALNTs transfer GalNAc to Mucins to form Tn antigens Homo sapiens GO:0004653 polypeptide N-acetylgalactosaminyltransferase activity TAS enables MF false ACCEPT Reactome's statement of GalNAc transfer, in the mucin Tn-antigen reaction and in the SARS-CoV-2 protein 3a glycosylation reaction respectively. reaction_mf_exact accepted_mf 1 human GALNT1 genes/human/GALNT1/GALNT1-ai-review.yaml GALNT1 Q10472 NCBITaxon:9606 Homo sapiens 13 R-HSA-9683760 GalNAc is transferred onto 3a yes reactome/R-HSA-9683760.md GalNAc is transferred onto 3a Homo sapiens GO:0004653 polypeptide N-acetylgalactosaminyltransferase activity TAS enables MF false ACCEPT Reactome's statement of GalNAc transfer, in the mucin Tn-antigen reaction and in the SARS-CoV-2 protein 3a glycosylation reaction respectively. reaction_mf_exact accepted_mf 1 human GALT genes/human/GALT/GALT-ai-review.yaml GALT P07902 NCBITaxon:9606 Homo sapiens 16 R-HSA-5610038 Defective GALT does not transfer UMP to Gal1P no GO:0008108 UDP-glucose:hexose-1-phosphate uridylyltransferase activity TAS enables MF false ACCEPT Reactome curation of the (defective) GALT uridylyl-transfer reaction; supports the same core molecular function. reaction_mf_exact accepted_mf reactome_disease_event|uncached_reactome 1 human GART genes/human/GART/GART-ai-review.yaml GART P22102 NCBITaxon:9606 Homo sapiens 22 R-HSA-73814 5-Phosphoribosylamine + Glycine + ATP => GAR + ADP + Pi yes reactome/R-HSA-73814.md 5-Phosphoribosylamine + Glycine + ATP => GAR + ADP + Pi Homo sapiens GO:0004637 phosphoribosylamine-glycine ligase activity TAS enables MF false ACCEPT Reactome (TAS) annotation of GAR synthetase activity: synthesis of GAR from 5-phosphoribosylamine, glycine and ATP. A core catalytic activity. reaction_mf_exact accepted_mf 1 human GART genes/human/GART/GART-ai-review.yaml GART P22102 NCBITaxon:9606 Homo sapiens 23 R-HSA-73810 FGAM + ATP => AIR + ADP + Pi yes reactome/R-HSA-73810.md FGAM + ATP => AIR + ADP + Pi Homo sapiens GO:0004641 phosphoribosylformylglycinamidine cyclo-ligase activity TAS enables MF false ACCEPT Reactome (TAS) annotation of AIR synthetase activity: irreversible ATP-dependent synthesis of AIR from FGAM. A core catalytic activity. reaction_mf_exact accepted_mf 1 human GART genes/human/GART/GART-ai-review.yaml GART P22102 NCBITaxon:9606 Homo sapiens 24 R-HSA-73813 GAR + 10-Formyl-THF => FGAR + THF yes reactome/R-HSA-73813.md GAR + 10-Formyl-THF => FGAR + THF Homo sapiens GO:0004644 phosphoribosylglycinamide formyltransferase activity TAS enables MF false ACCEPT Reactome (TAS) annotation of GAR transformylase activity: folate-dependent formyl transfer from 10-formyl-THF to GAR to give FGAR. A core catalytic activity. reaction_mf_exact accepted_mf 1 human GBE1 genes/human/GBE1/GBE1-ai-review.yaml GBE1 Q04446 NCBITaxon:9606 Homo sapiens 14 R-HSA-3878762 Defective GBE1 does not catalyze branch formation in growing glycogen chains (liver) yes reactome/R-HSA-3878762.md Defective GBE1 does not catalyze branch formation in growing glycogen chains (liver) Homo sapiens GO:0003844 1,4-alpha-glucan branching enzyme activity TAS enables MF false ACCEPT Reactome TAS annotation (from the GSD IV disease reaction, in which defective GBE1 fails to catalyze branch formation) to 1,4-alpha-glucan branching enzyme activity. Reinforces the core catalytic function via pathway knowledge. reaction_mf_exact accepted_mf reactome_disease_event 1 human GCG genes/human/GCG/GCG-ai-review.yaml GCG P01275 NCBITaxon:9606 Homo sapiens 30 R-HSA-381612 GLP1R binds GLP1 yes reactome/R-HSA-381612.md GLP1R binds GLP1 Homo sapiens GO:0048018 receptor ligand activity TAS enables MF false ACCEPT "GLP-1 acts as the agonist ligand of GLP1R (Reactome reaction ""GLP1R binds GLP1""). Correct core molecular function." reaction_mf_exact accepted_mf 1 human GCK genes/human/GCK/GCK-ai-review.yaml GCK P35557 NCBITaxon:9606 Homo sapiens 34 R-HSA-5621918 Defective GCK does not phosphorylate Glc to form G6P yes reactome/R-HSA-5621918.md Defective GCK does not phosphorylate Glc to form G6P Homo sapiens GO:0004340 glucokinase activity TAS enables MF false ACCEPT Reactome TAS annotation of glucokinase activity, from the reaction describing defective GCK failing to phosphorylate glucose to G6P. Correct core molecular function. reaction_mf_exact accepted_mf reactome_disease_event 1 human GCK genes/human/GCK/GCK-ai-review.yaml GCK P35557 NCBITaxon:9606 Homo sapiens 35 R-HSA-70420 HK1,2,3,GCK,HKDC1 phosphorylate Glc to form G6P yes reactome/R-HSA-70420.md HK1,2,3,GCK,HKDC1 phosphorylate Glc to form G6P Homo sapiens GO:0004340 glucokinase activity TAS enables MF false ACCEPT Reactome TAS annotation of glucokinase activity, from the reaction in which HK1,2,3,GCK,HKDC1 phosphorylate glucose to form G6P. Correct core molecular function. reaction_mf_exact accepted_mf local_module_mf|multi_gene_reactome_id 2 glycolysis_investment_phase:GO:0004396 hexokinase activity human GCNT1 genes/human/GCNT1/GCNT1-ai-review.yaml GCNT1 Q02742 NCBITaxon:9606 Homo sapiens 7 R-HSA-914012 GCNT1 transfers GlcNAc to Core 1 glycoproteins forming Core 2 glycoproteins yes reactome/R-HSA-914012.md GCNTs transfer GlcNAc from UDP-GlcNAc to Core 1 mucins Homo sapiens GO:0003829 beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity TAS enables MF false ACCEPT Reactome's statement of the core 2 branching reaction, matching the assayed activity. reaction_mf_exact accepted_mf 1 human GFPT1 genes/human/GFPT1/GFPT1-ai-review.yaml GFPT1 Q06210 NCBITaxon:9606 Homo sapiens 15 R-HSA-4085027 Defective GFPT1 does not transfer an amino group from L-Gln to F6P to form GlcN6P yes reactome/R-HSA-4085027.md Defective GFPT1 does not transfer an amino group from L-Gln to F6P to form GlcN6P Homo sapiens GO:0004360 L-glutamine:D-fructose-6-phosphate transaminase (isomerizing) activity TAS enables MF false ACCEPT Core molecular function, curated by Reactome (this reaction annotation describes the defective-GFPT1 case but asserts the wild-type activity of transferring an amino group from L-Gln to F6P to form GlcN6P). reaction_mf_exact accepted_mf reactome_disease_event 1 human GFPT1 genes/human/GFPT1/GFPT1-ai-review.yaml GFPT1 Q06210 NCBITaxon:9606 Homo sapiens 16 R-HSA-449715 GFPT1,2 transfer an amino group from L-Gln to F6P to form GlcN6P yes reactome/R-HSA-449715.md GFPT1,2 transfer an amino group from L-Gln to F6P to form GlcN6P Homo sapiens GO:0004360 L-glutamine:D-fructose-6-phosphate transaminase (isomerizing) activity TAS enables MF false ACCEPT Core molecular function, curated by Reactome (GFPT1,2 transfer an amino group from L-Gln to F6P to form GlcN6P). Consistent with all other GO:0004360 annotations. reaction_mf_exact accepted_mf multi_gene_reactome_id 2 human GFPT2 genes/human/GFPT2/GFPT2-ai-review.yaml GFPT2 O94808 NCBITaxon:9606 Homo sapiens 15 R-HSA-449715 GFPT1,2 transfer an amino group from L-Gln to F6P to form GlcN6P yes reactome/R-HSA-449715.md GFPT1,2 transfer an amino group from L-Gln to F6P to form GlcN6P Homo sapiens GO:0004360 L-glutamine:D-fructose-6-phosphate transaminase (isomerizing) activity TAS enables MF false ACCEPT "Reactome TAS for the GFAT2 reaction ""GFPT1,2 transfer an amino group from L-Gln to F6P to form GlcN6P"". This is the core catalytic function; consistent with the experimental IDA and the EC 2.6.1.16 assignment." reaction_mf_exact accepted_mf multi_gene_reactome_id 2 human GGT1 genes/human/GGT1/GGT1-ai-review.yaml GGT1 P19440 NCBITaxon:9606 Homo sapiens 13 R-HSA-5602984 Defective GGT1 does not hydrolyse glutamate from AFXBO-SG, AFNBO-SG yes reactome/R-HSA-5602984.md Defective GGT1 does not hydrolyse glutamate from AFXBO-SG, AFNBO-SG Homo sapiens GO:0036374 glutathione gamma-glutamate hydrolase TAS enables MF false ACCEPT Reactome TAS for the core glutathione hydrolase MF (this reaction models defective GGT1 failing to hydrolyse aflatoxin-GSH conjugates, but is annotated to the normal MF GGT1 enables). reaction_mf_exact accepted_mf reactome_disease_event 1 human GGT1 genes/human/GGT1/GGT1-ai-review.yaml GGT1 P19440 NCBITaxon:9606 Homo sapiens 14 R-HSA-9035966 Defective GGT1 does not hydrolyse GSH yes reactome/R-HSA-9035966.md Defective GGT1 does not hydrolyse GSH Homo sapiens GO:0036374 glutathione gamma-glutamate hydrolase TAS enables MF false ACCEPT Reactome TAS for the core glutathione hydrolase MF (defective-GGT1 GSH-hydrolysis reaction, annotated to the normal enabled MF). reaction_mf_exact accepted_mf reactome_disease_event 1 human GLB1 genes/human/GLB1/GLB1-ai-review.yaml GLB1 P16278 NCBITaxon:9606 Homo sapiens 17 R-HSA-2265534 Defective GLB1 does not hydrolyse a glycosaminoglycan yes reactome/R-HSA-2265534.md Defective GLB1 does not hydrolyse a glycosaminoglycan Homo sapiens GO:0004565 beta-galactosidase activity TAS enables MF false ACCEPT Reactome TAS annotation (from the pathway describing defective GLB1 failing to hydrolyze a glycosaminoglycan) supporting beta-galactosidase activity. reaction_mf_exact accepted_mf reactome_disease_event 1 human GLB1 genes/human/GLB1/GLB1-ai-review.yaml GLB1 P16278 NCBITaxon:9606 Homo sapiens 18 R-HSA-9036061 Defective GLB1 does not hydrolyse linker chain(2) yes reactome/R-HSA-9036061.md Defective GLB1 does not hydrolyse linker chain(2) Homo sapiens GO:0004565 beta-galactosidase activity TAS enables MF false ACCEPT Reactome TAS annotation supporting beta-galactosidase activity. reaction_mf_exact accepted_mf reactome_disease_event 1 human GM2A genes/human/GM2A/GM2A-ai-review.yaml GM2A P17900 NCBITaxon:9606 Homo sapiens 26 R-HSA-1605717 GM2A binds and mobilizes ligands yes reactome/R-HSA-1605717.md GM2A binds and mobilizes ligands Homo sapiens GO:0030290 sphingolipid activator protein activity TAS enables MF false ACCEPT Reactome traceable annotation to sphingolipid activator protein activity, the family-level cofactor activity for lysosomal sphingolipid degradation. Accurate for GM2A (SAP-3). reaction_mf_exact accepted_mf 1 human GNE genes/human/GNE/GNE-ai-review.yaml GNE Q9Y223 NCBITaxon:9606 Homo sapiens 13 R-HSA-4088338 Defective GNE does not hydrolyse UDP-GlcNAc yes reactome/R-HSA-4088338.md Defective GNE does not hydrolyse UDP-GlcNAc Homo sapiens GO:0008761 UDP-N-acetylglucosamine 2-epimerase activity TAS enables MF false ACCEPT "Reactome TAS for the epimerase activity (reaction ""Defective GNE does not hydrolyse UDP-GlcNAc""). Corroborates the core epimerase function. Accept." reaction_mf_exact accepted_mf reactome_disease_event 1 human GNE genes/human/GNE/GNE-ai-review.yaml GNE Q9Y223 NCBITaxon:9606 Homo sapiens 14 R-HSA-4088322 Defective GNE does not phosphorylate ManNAc to ManNAc-6-P yes reactome/R-HSA-4088322.md Defective GNE does not phosphorylate ManNAc to ManNAc-6-P Homo sapiens GO:0009384 N-acylmannosamine kinase activity TAS enables MF false ACCEPT "Reactome TAS for the ManNAc kinase activity (reaction ""Defective GNE does not phosphorylate ManNAc to ManNAc-6-P""). Corroborates the core kinase function. Accept." reaction_mf_exact accepted_mf reactome_disease_event 1 human GNPAT genes/human/GNPAT/GNPAT-ai-review.yaml GNPAT O15228 NCBITaxon:9606 Homo sapiens 14 R-HSA-1483002 DHAP is converted to 1-acyl GO3P by GNPAT yes reactome/R-HSA-1483002.md DHAP is converted to 1-acyl GO3P by GNPAT Homo sapiens GO:0016287 glycerone-phosphate O-acyltransferase activity TAS enables MF false ACCEPT Reactome traceable annotation of the core glycerone-phosphate O-acyltransferase activity (DHAP -> 1-acyl GO3P). Correct core function. reaction_mf_exact accepted_mf 1 human GNPAT genes/human/GNPAT/GNPAT-ai-review.yaml GNPAT O15228 NCBITaxon:9606 Homo sapiens 15 R-HSA-75879 palmitoyl-CoA + DHAP => 1-palmitoylglycerone phosphate + CoASH yes reactome/R-HSA-75879.md palmitoyl-CoA + DHAP => 1-palmitoylglycerone phosphate + CoASH Homo sapiens GO:0016287 glycerone-phosphate O-acyltransferase activity TAS enables MF false ACCEPT Reactome traceable annotation of the core glycerone-phosphate O-acyltransferase activity (palmitoyl-CoA + DHAP -> 1-palmitoylglycerone phosphate + CoASH). Correct core function. reaction_mf_exact accepted_mf 1 human GNS genes/human/GNS/GNS-ai-review.yaml GNS P15586 NCBITaxon:9606 Homo sapiens 12 R-HSA-2263495 Defective GNS does not hydrolyse 6-sulfate from GlcNAc6S yes reactome/R-HSA-2263495.md Defective GNS does not hydrolyse 6-sulfate from GlcNAc6S Homo sapiens GO:0008449 N-acetylglucosamine-6-sulfatase activity TAS enables MF false ACCEPT TAS annotation (Reactome) to the specific sulfatase activity, in the context of the keratan sulfate degradation / MPS IIID pathway. Core molecular function; accept. reaction_mf_exact accepted_mf reactome_disease_event 1 human GPD2 genes/human/GPD2/GPD2-ai-review.yaml GPD2 P43304 NCBITaxon:9606 Homo sapiens 12 R-HSA-188467 Gly-3-P+FAD->DHAP+FADH2 (catalyzed by mitochondrial Gly-Phos dehydrogenase) yes reactome/R-HSA-188467.md Gly-3-P+FAD->DHAP+FADH2 (catalyzed by mitochondrial Gly-Phos dehydrogenase) Homo sapiens GO:0004368 glycerol-3-phosphate dehydrogenase (quinone) activity TAS enables MF false ACCEPT Reactome traceable-author annotation of the core catalytic activity, consistent with the experimental IDA and the UniProt catalytic activity. reaction_mf_exact accepted_mf 1 human GPI genes/human/GPI/GPI-ai-review.yaml GPI P06744 NCBITaxon:9606 Homo sapiens 35 R-HSA-70471 GPI dimer isomerizes G6P yes reactome/R-HSA-70471.md GPI dimer isomerizes G6P Homo sapiens GO:0004347 glucose-6-phosphate isomerase activity TAS enables MF false ACCEPT Reactome traceable assertion (GPI dimer isomerizes G6P) for the core catalytic activity. reaction_mf_exact accepted_mf local_module_mf 1 glycolysis_investment_phase:GO:0004347 glucose-6-phosphate isomerase activity human GPX4 genes/human/GPX4/GPX4-ai-review.yaml GPX4 P36969 NCBITaxon:9606 Homo sapiens 26 R-HSA-9018868 GPX4-2 reduces 18(S)-HpEPE to 18(S)-HEPE yes reactome/R-HSA-9018868.md GPX4-2 reduces 18(S)-HpEPE to 18(S)-HEPE Homo sapiens GO:0047066 phospholipid-hydroperoxide glutathione peroxidase activity TAS enables MF false ACCEPT Reactome reaction showing GPX4-2 reduces 18(S)-HpEPE to 18(S)-HEPE. reaction_mf_exact accepted_mf 1 human GPX4 genes/human/GPX4/GPX4-ai-review.yaml GPX4 P36969 NCBITaxon:9606 Homo sapiens 27 R-HSA-9018895 GPX4-2 reduces 18(R)-HpEPE to 18(R)-HEPE yes reactome/R-HSA-9018895.md GPX4-2 reduces 18(R)-HpEPE to 18(R)-HEPE Homo sapiens GO:0047066 phospholipid-hydroperoxide glutathione peroxidase activity TAS enables MF false ACCEPT Reactome reaction showing GPX4-2 reduces 18(R)-HpEPE to 18(R)-HEPE. reaction_mf_exact accepted_mf 1 human GPX4 genes/human/GPX4/GPX4-ai-review.yaml GPX4 P36969 NCBITaxon:9606 Homo sapiens 28 R-HSA-9020271 GPX4-2 reduces 17(R)-Hp-DHA to 17(R)-HDHA yes reactome/R-HSA-9020271.md GPX4-2 reduces 17(R)-Hp-DHA to 17(R)-HDHA Homo sapiens GO:0047066 phospholipid-hydroperoxide glutathione peroxidase activity TAS enables MF false ACCEPT Reactome reaction showing GPX4-2 reduces 17(R)-Hp-DHA to 17(R)-HDHA. reaction_mf_exact accepted_mf 1 human GPX4 genes/human/GPX4/GPX4-ai-review.yaml GPX4 P36969 NCBITaxon:9606 Homo sapiens 29 R-HSA-9020273 GPX4-2 reduces 17(S)-Hp-DHA to 17(S)-HDHA yes reactome/R-HSA-9020273.md GPX4-2 reduces 17(S)-Hp-DHA to 17(S)-HDHA Homo sapiens GO:0047066 phospholipid-hydroperoxide glutathione peroxidase activity TAS enables MF false ACCEPT Reactome reaction showing GPX4-2 reduces 17(S)-Hp-DHA to 17(S)-HDHA. reaction_mf_exact accepted_mf 1 human GRHPR genes/human/GRHPR/GRHPR-ai-review.yaml GRHPR Q9UBQ7 NCBITaxon:9606 Homo sapiens 15 R-HSA-389826 glyoxylate + NADPH + H+ => glycolate + NADP+ yes reactome/R-HSA-389826.md glyoxylate + NADPH + H+ => glycolate + NADP+ Homo sapiens GO:0030267 glyoxylate reductase (NADPH) activity TAS enables MF false ACCEPT Reactome TAS annotation of glyoxylate reductase (NADPH) activity for the reaction glyoxylate + NADPH + H+ => glycolate + NADP+, the enzyme's core catalytic step. reaction_mf_exact accepted_mf local_module_mf 1 glyoxylate_oxalate_metabolism:GO:0030267 glyoxylate reductase (NADPH) activity human GSK3B genes/human/GSK3B/GSK3B-ai-review.yaml GSK3B P49841 NCBITaxon:9606 Homo sapiens 142 R-HSA-195283 Phosphorylation of phospho- (Ser45, Thr41) beta-catenin at Ser37 by GSK-3 yes reactome/R-HSA-195283.md Phosphorylation of phospho- (Ser45, Thr41) beta-catenin at Ser37 by GSK-3 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT protein serine/threonine kinase activity is retained as a core molecular-function annotation for GSK3B; it captures activity or binding specificity within the synthesized core biology: GSK3B catalytic kinase activity, substrate phosphorylation, Wnt/beta-catenin destruction-complex signaling, glycogen/insulin regulation, tau phosphorylation, protein-turnover control, and the cytosolic/nuclear sites where these reactions occur. reaction_mf_exact accepted_mf 1 human GSK3B genes/human/GSK3B/GSK3B-ai-review.yaml GSK3B P49841 NCBITaxon:9606 Homo sapiens 143 R-HSA-195287 Phosphorylation of phospho-(Ser45 ) at Thr 41 by GSK-3 yes reactome/R-HSA-195287.md Phosphorylation of phospho-(Ser45 ) at Thr 41 by GSK-3 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT protein serine/threonine kinase activity is retained as a core molecular-function annotation for GSK3B; it captures activity or binding specificity within the synthesized core biology: GSK3B catalytic kinase activity, substrate phosphorylation, Wnt/beta-catenin destruction-complex signaling, glycogen/insulin regulation, tau phosphorylation, protein-turnover control, and the cytosolic/nuclear sites where these reactions occur. reaction_mf_exact accepted_mf 1 human GSK3B genes/human/GSK3B/GSK3B-ai-review.yaml GSK3B P49841 NCBITaxon:9606 Homo sapiens 144 R-HSA-195300 Phosphorylation of phospho-(Ser45,Thr41,Ser37) at Ser33 by GSK-3 yes reactome/R-HSA-195300.md Phosphorylation of phospho-(Ser45,Thr41,Ser37) at Ser33 by GSK-3 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT protein serine/threonine kinase activity is retained as a core molecular-function annotation for GSK3B; it captures activity or binding specificity within the synthesized core biology: GSK3B catalytic kinase activity, substrate phosphorylation, Wnt/beta-catenin destruction-complex signaling, glycogen/insulin regulation, tau phosphorylation, protein-turnover control, and the cytosolic/nuclear sites where these reactions occur. reaction_mf_exact accepted_mf 1 human GSK3B genes/human/GSK3B/GSK3B-ai-review.yaml GSK3B P49841 NCBITaxon:9606 Homo sapiens 145 R-HSA-201677 Phosphorylation of LRP5/6 cytoplasmic domain by membrane-associated GSK3beta yes reactome/R-HSA-201677.md Phosphorylation of LRP5/6 cytoplasmic domain by membrane-associated GSK3beta Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT protein serine/threonine kinase activity is retained as a core molecular-function annotation for GSK3B; it captures activity or binding specificity within the synthesized core biology: GSK3B catalytic kinase activity, substrate phosphorylation, Wnt/beta-catenin destruction-complex signaling, glycogen/insulin regulation, tau phosphorylation, protein-turnover control, and the cytosolic/nuclear sites where these reactions occur. reaction_mf_exact accepted_mf 1 human GSK3B genes/human/GSK3B/GSK3B-ai-review.yaml GSK3B P49841 NCBITaxon:9606 Homo sapiens 146 R-HSA-3371435 Constitutive phosphorylation by GSK3 yes reactome/R-HSA-3371435.md Constitutive phosphorylation by GSK3 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT protein serine/threonine kinase activity is retained as a core molecular-function annotation for GSK3B; it captures activity or binding specificity within the synthesized core biology: GSK3B catalytic kinase activity, substrate phosphorylation, Wnt/beta-catenin destruction-complex signaling, glycogen/insulin regulation, tau phosphorylation, protein-turnover control, and the cytosolic/nuclear sites where these reactions occur. reaction_mf_exact accepted_mf 1 human GSK3B genes/human/GSK3B/GSK3B-ai-review.yaml GSK3B P49841 NCBITaxon:9606 Homo sapiens 147 R-HSA-399951 Phosphorylation of CRMPs by GSK3beta yes reactome/R-HSA-399951.md Phosphorylation of CRMPs by GSK3beta Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT protein serine/threonine kinase activity is retained as a core molecular-function annotation for GSK3B; it captures activity or binding specificity within the synthesized core biology: GSK3B catalytic kinase activity, substrate phosphorylation, Wnt/beta-catenin destruction-complex signaling, glycogen/insulin regulation, tau phosphorylation, protein-turnover control, and the cytosolic/nuclear sites where these reactions occur. reaction_mf_exact accepted_mf 1 human GSK3B genes/human/GSK3B/GSK3B-ai-review.yaml GSK3B P49841 NCBITaxon:9606 Homo sapiens 148 R-HSA-5610732 GSK3 phosphorylates p-GLI3 yes reactome/R-HSA-5610732.md GSK3 phosphorylates p-GLI3 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT protein serine/threonine kinase activity is retained as a core molecular-function annotation for GSK3B; it captures activity or binding specificity within the synthesized core biology: GSK3B catalytic kinase activity, substrate phosphorylation, Wnt/beta-catenin destruction-complex signaling, glycogen/insulin regulation, tau phosphorylation, protein-turnover control, and the cytosolic/nuclear sites where these reactions occur. reaction_mf_exact accepted_mf 1 human GSK3B genes/human/GSK3B/GSK3B-ai-review.yaml GSK3B P49841 NCBITaxon:9606 Homo sapiens 149 R-HSA-9683664 GSK3 phosphorylates Nucleoprotein yes reactome/R-HSA-9683664.md GSK3 phosphorylates Nucleoprotein Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT protein serine/threonine kinase activity is retained as a core molecular-function annotation for GSK3B; it captures activity or binding specificity within the synthesized core biology: GSK3B catalytic kinase activity, substrate phosphorylation, Wnt/beta-catenin destruction-complex signaling, glycogen/insulin regulation, tau phosphorylation, protein-turnover control, and the cytosolic/nuclear sites where these reactions occur. reaction_mf_exact accepted_mf 1 human GSK3B genes/human/GSK3B/GSK3B-ai-review.yaml GSK3B P49841 NCBITaxon:9606 Homo sapiens 150 R-HSA-9729260 GSK3 phosphorylates nucleoprotein yes reactome/R-HSA-9729260.md GSK3 phosphorylates nucleoprotein Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT protein serine/threonine kinase activity is retained as a core molecular-function annotation for GSK3B; it captures activity or binding specificity within the synthesized core biology: GSK3B catalytic kinase activity, substrate phosphorylation, Wnt/beta-catenin destruction-complex signaling, glycogen/insulin regulation, tau phosphorylation, protein-turnover control, and the cytosolic/nuclear sites where these reactions occur. reaction_mf_exact accepted_mf 1 human GSK3B genes/human/GSK3B/GSK3B-ai-review.yaml GSK3B P49841 NCBITaxon:9606 Homo sapiens 151 R-HSA-9762094 GSK3B phosphorylates p-NFE2L2 yes reactome/R-HSA-9762094.md GSK3B phosphorylates p-NFE2L2 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT protein serine/threonine kinase activity is retained as a core molecular-function annotation for GSK3B; it captures activity or binding specificity within the synthesized core biology: GSK3B catalytic kinase activity, substrate phosphorylation, Wnt/beta-catenin destruction-complex signaling, glycogen/insulin regulation, tau phosphorylation, protein-turnover control, and the cytosolic/nuclear sites where these reactions occur. reaction_mf_exact accepted_mf 1 human GSK3B genes/human/GSK3B/GSK3B-ai-review.yaml GSK3B P49841 NCBITaxon:9606 Homo sapiens 152 R-HSA-9824995 GSK3B phosphorylates p-S409 MITF-M at S397, S401 and S405 yes reactome/R-HSA-9824995.md GSK3B phosphorylates p-S409 MITF-M at S397, S401 and S405 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT protein serine/threonine kinase activity is retained as a core molecular-function annotation for GSK3B; it captures activity or binding specificity within the synthesized core biology: GSK3B catalytic kinase activity, substrate phosphorylation, Wnt/beta-catenin destruction-complex signaling, glycogen/insulin regulation, tau phosphorylation, protein-turnover control, and the cytosolic/nuclear sites where these reactions occur. reaction_mf_exact accepted_mf 1 human GSK3B genes/human/GSK3B/GSK3B-ai-review.yaml GSK3B P49841 NCBITaxon:9606 Homo sapiens 153 R-HSA-9824999 GSK3B phosphorylates p-S73 MITF-M at residue S69 yes reactome/R-HSA-9824999.md GSK3B phosphorylates p-S73 MITF-M at residue S69 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT protein serine/threonine kinase activity is retained as a core molecular-function annotation for GSK3B; it captures activity or binding specificity within the synthesized core biology: GSK3B catalytic kinase activity, substrate phosphorylation, Wnt/beta-catenin destruction-complex signaling, glycogen/insulin regulation, tau phosphorylation, protein-turnover control, and the cytosolic/nuclear sites where these reactions occur. reaction_mf_exact accepted_mf 1 human GSK3B genes/human/GSK3B/GSK3B-ai-review.yaml GSK3B P49841 NCBITaxon:9606 Homo sapiens 154 R-HSA-9929360 GSK3B phosphorylates CD274 yes reactome/R-HSA-9929360.md GSK3B phosphorylates CD274 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT protein serine/threonine kinase activity is retained as a core molecular-function annotation for GSK3B; it captures activity or binding specificity within the synthesized core biology: GSK3B catalytic kinase activity, substrate phosphorylation, Wnt/beta-catenin destruction-complex signaling, glycogen/insulin regulation, tau phosphorylation, protein-turnover control, and the cytosolic/nuclear sites where these reactions occur. reaction_mf_exact accepted_mf 1 human GSK3B genes/human/GSK3B/GSK3B-ai-review.yaml GSK3B P49841 NCBITaxon:9606 Homo sapiens 155 R-HSA-9943675 GSK3B phosphorylates CDC25A yes reactome/R-HSA-9943675.md GSK3B phosphorylates CDC25A Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT protein serine/threonine kinase activity is retained as a core molecular-function annotation for GSK3B; it captures activity or binding specificity within the synthesized core biology: GSK3B catalytic kinase activity, substrate phosphorylation, Wnt/beta-catenin destruction-complex signaling, glycogen/insulin regulation, tau phosphorylation, protein-turnover control, and the cytosolic/nuclear sites where these reactions occur. reaction_mf_exact accepted_mf 1 human GSK3B genes/human/GSK3B/GSK3B-ai-review.yaml GSK3B P49841 NCBITaxon:9606 Homo sapiens 156 R-NUL-209146 Murine Axin1 is further phosphorylated by Human GSK3beta yes reactome/R-NUL-209146.md Murine Axin1 is further phosphorylated by Human GSK3beta Mus musculus GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT protein serine/threonine kinase activity is retained as a core molecular-function annotation for GSK3B; it captures activity or binding specificity within the synthesized core biology: GSK3B catalytic kinase activity, substrate phosphorylation, Wnt/beta-catenin destruction-complex signaling, glycogen/insulin regulation, tau phosphorylation, protein-turnover control, and the cytosolic/nuclear sites where these reactions occur. reaction_mf_exact accepted_mf 1 human GSK3B genes/human/GSK3B/GSK3B-ai-review.yaml GSK3B P49841 NCBITaxon:9606 Homo sapiens 157 R-NUL-9008636 GSK3B phosphorylates Runx2 yes reactome/R-NUL-9008636.md GSK3B phosphorylates Runx2 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT protein serine/threonine kinase activity is retained as a core molecular-function annotation for GSK3B; it captures activity or binding specificity within the synthesized core biology: GSK3B catalytic kinase activity, substrate phosphorylation, Wnt/beta-catenin destruction-complex signaling, glycogen/insulin regulation, tau phosphorylation, protein-turnover control, and the cytosolic/nuclear sites where these reactions occur. reaction_mf_exact accepted_mf 1 human GUSB genes/human/GUSB/GUSB-ai-review.yaml GUSB P08236 NCBITaxon:9606 Homo sapiens 13 R-HSA-2318373 Defective GUSB does not hydrolyse (HA)2 yes reactome/R-HSA-2318373.md Defective GUSB does not hydrolyse (HA)2 Homo sapiens GO:0004566 beta-glucuronidase activity TAS enables MF false ACCEPT Reactome (TAS) assertion of beta-glucuronidase activity in the context of hyaluronan/GAG degradation reactions. Consistent with the core molecular function. reaction_mf_exact accepted_mf reactome_disease_event 1 human GUSB genes/human/GUSB/GUSB-ai-review.yaml GUSB P08236 NCBITaxon:9606 Homo sapiens 14 R-HSA-9036068 Defective GUSB does not hydrolyse GlcA-β1,3-GlcNAc yes reactome/R-HSA-9036068.md Defective GUSB does not hydrolyse GlcA-β1,3-GlcNAc Homo sapiens GO:0004566 beta-glucuronidase activity TAS enables MF false ACCEPT Reactome (TAS) assertion of beta-glucuronidase activity (GlcA-beta1,3-GlcNAc hydrolysis reaction). Consistent with the core molecular function. reaction_mf_exact accepted_mf reactome_disease_event 1 human GUSB genes/human/GUSB/GUSB-ai-review.yaml GUSB P08236 NCBITaxon:9606 Homo sapiens 15 R-HSA-9036070 Defective GUSB does not hydrolyse CS/HS precursor yes reactome/R-HSA-9036070.md Defective GUSB does not hydrolyse CS/HS precursor Homo sapiens GO:0004566 beta-glucuronidase activity TAS enables MF false ACCEPT Reactome (TAS) assertion of beta-glucuronidase activity (CS/HS precursor hydrolysis). Consistent with the core molecular function. reaction_mf_exact accepted_mf reactome_disease_event 1 human GYS2 genes/human/GYS2/GYS2-ai-review.yaml GYS2 P54840 NCBITaxon:9606 Homo sapiens 12 R-HSA-3780994 GYS2 catalyzes the incorporation of phosphoglucose into glycogen-GYG2 no GO:0061547 glycogen synthase activity, transferring glucose-1-phosphate TAS enables MF false ACCEPT Reactome-curated minor activity in which GYS2 incorporates phosphoglucose into glycogen, the origin of glycogen phosphomonoesters. reaction_mf_exact accepted_mf uncached_reactome 1 human HBS1L genes/human/HBS1L/HBS1L-ai-review.yaml HBS1L Q9Y450 NCBITaxon:9606 Homo sapiens 17 R-HSA-9954919 Reactome ribosome rescue reaction (HBS1L) yes reactome/R-HSA-9954919.md ABCE1:ATP binds PELO:HBS1L-1:GTP:80S ribosome:non-stop mRNA:peptidyl-tRNA with nascent peptide and HBS1L-1:GDP is released Homo sapiens GO:0003924 GTPase activity TAS enables MF false ACCEPT Reactome curated GTPase activity in the ribosome-rescue pathway. reaction_mf_exact accepted_mf 1 human HDAC4 genes/human/HDAC4/HDAC4-ai-review.yaml HDAC4 P56524 NCBITaxon:9606 Homo sapiens 54 R-HSA-8952069 HDAC4 deacetylates RUNX3 yes reactome/R-HSA-8952069.md HDAC4 deacetylates RUNX3 Homo sapiens GO:0033558 protein lysine deacetylase activity TAS enables MF false ACCEPT This Reactome annotation for HDAC4 deacetylation of RUNX3 correctly identifies protein lysine deacetylase activity toward a non-histone substrate. reaction_mf_exact accepted_mf 1 human HEXA genes/human/HEXA/HEXA-ai-review.yaml HEXA P06865 NCBITaxon:9606 Homo sapiens 22 R-HSA-9035976 Defective HEXA does not cleave the terminall GalNAc from small HA fragments yes reactome/R-HSA-9035976.md Defective HEXA does not cleave the terminall GalNAc from small HA fragments Homo sapiens GO:0004563 beta-N-acetylhexosaminidase activity TAS enables MF false ACCEPT Reactome (TAS) assertion of beta-N-acetylhexosaminidase activity. The source reaction describes a defective HEXA variant in hyaluronan metabolism, but the molecular function assigned (beta-N-acetylhexosaminidase activity) is the correct wild-type activity. reaction_mf_exact accepted_mf reactome_disease_event 1 human HEXB genes/human/HEXB/HEXB-ai-review.yaml HEXB P07686 NCBITaxon:9606 Homo sapiens 32 R-HSA-9035982 Defective HEXB does not cleave the terminal GalNAc from HA fragments yes reactome/R-HSA-9035982.md Defective HEXB does not cleave the terminal GalNAc from HA fragments Homo sapiens GO:0004563 beta-N-acetylhexosaminidase activity TAS enables MF false ACCEPT Reactome TAS for the core catalytic activity (in the context of defective HEXB failing to cleave terminal GalNAc from hyaluronan fragments). reaction_mf_exact accepted_mf reactome_disease_event 1 human HGSNAT genes/human/HGSNAT/HGSNAT-ai-review.yaml HGSNAT Q68CP4 NCBITaxon:9606 Homo sapiens 7 R-HSA-2263492 Defective HGSNAT does not acetylate Heparan chain(1) yes reactome/R-HSA-2263492.md Defective HGSNAT does not acetylate Heparan chain(1) Homo sapiens GO:0015019 heparan-alpha-glucosaminide N-acetyltransferase activity TAS enables MF false ACCEPT "Reactome traceable-author annotation of the exact-match N-acetyltransferase activity, from a reaction representing HGSNAT acetylation of the heparan chain (the ""defective"" reaction node modelling loss of this activity in MPS IIIC). Supports the core catalytic function." reaction_mf_exact accepted_mf reactome_disease_event 1 human HGSNAT genes/human/HGSNAT/HGSNAT-ai-review.yaml HGSNAT Q68CP4 NCBITaxon:9606 Homo sapiens 8 R-HSA-9036056 Defective HGSNAT does not acetylate Heparan sulfate chain(3) yes reactome/R-HSA-9036056.md Defective HGSNAT does not acetylate Heparan sulfate chain(3) Homo sapiens GO:0015019 heparan-alpha-glucosaminide N-acetyltransferase activity TAS enables MF false ACCEPT Reactome traceable-author annotation of the exact-match N-acetyltransferase activity (second Reactome reaction node). Redundant with the other MF annotations and supports the core catalytic function. reaction_mf_exact accepted_mf reactome_disease_event 1 human HIBCH genes/human/HIBCH/HIBCH-ai-review.yaml HIBCH Q6NVY1 NCBITaxon:9606 Homo sapiens 9 R-HSA-9916727 HIBCH mutants don't synthesize beta-hydroxyisobutyrate yes reactome/R-HSA-9916727.md HIBCH mutants don't synthesize beta-hydroxyisobutyrate Homo sapiens GO:0003860 3-hydroxyisobutyryl-CoA hydrolase activity TAS enables MF false ACCEPT Reactome (TAS) annotation of 3-hydroxyisobutyryl-CoA hydrolase activity in the context of the HIBCH-deficiency disease pathway (pathogenic variants that lose the ability to synthesize beta-hydroxyisobutyrate). reaction_mf_exact accepted_mf 1 human HK1 genes/human/HK1/HK1-ai-review.yaml HK1 P19367 NCBITaxon:9606 Homo sapiens 41 R-HSA-5621888 Defective HK1 does not phosphorylate Glc to form G6P yes reactome/R-HSA-5621888.md Defective HK1 does not phosphorylate Glc to form G6P Homo sapiens GO:0004340 glucokinase activity TAS enables MF false ACCEPT Reactome TAS for glucose-phosphorylating (glucokinase) activity (from the defective-HK1 disease reaction). Reflects HK1's core glucose-to-G6P reaction. reaction_mf_exact accepted_mf reactome_disease_event 1 human HK1 genes/human/HK1/HK1-ai-review.yaml HK1 P19367 NCBITaxon:9606 Homo sapiens 42 R-HSA-70420 HK1,2,3,GCK,HKDC1 phosphorylate Glc to form G6P yes reactome/R-HSA-70420.md HK1,2,3,GCK,HKDC1 phosphorylate Glc to form G6P Homo sapiens GO:0004340 glucokinase activity TAS enables MF false ACCEPT Reactome TAS (HK1,2,3,GCK,HKDC1 phosphorylate Glc to form G6P) for glucose-phosphorylating activity. HK1 is an annotated member of the enzyme set catalyzing glucose to G6P. reaction_mf_exact accepted_mf local_module_mf|multi_gene_reactome_id 2 glycolysis_investment_phase:GO:0004396 hexokinase activity human HLCS genes/human/HLCS/HLCS-ai-review.yaml HLCS P50747 NCBITaxon:9606 Homo sapiens 15 R-HSA-2993447 HLCS biotinylates 6x(PCCA:PCCB) no GO:0004077 biotin--[biotin carboxyl-carrier protein] ligase activity TAS enables MF false ACCEPT "Reactome (TAS) biotin--protein ligase activity for the reaction ""HLCS biotinylates 6x(PCCA:PCCB)"" (biotinylation of propionyl-CoA carboxylase). Correct core molecular function on a genuine substrate." reaction_mf_exact accepted_mf local_module_mf|uncached_reactome 1 biotin_cycle:GO:0004077 biotin--[biotin carboxyl-carrier protein] ligase activity human HLCS genes/human/HLCS/HLCS-ai-review.yaml HLCS P50747 NCBITaxon:9606 Homo sapiens 16 R-HSA-2993799 HLCS biotinylates 6xMCCC1:6xMCCC2 no GO:0004077 biotin--[biotin carboxyl-carrier protein] ligase activity TAS enables MF false ACCEPT "Reactome (TAS) biotin--protein ligase activity for ""HLCS biotinylates 6xMCCC1:6xMCCC2"" (biotinylation of 3-methylcrotonyl-CoA carboxylase). Correct core function on a genuine substrate." reaction_mf_exact accepted_mf uncached_reactome 1 human HLCS genes/human/HLCS/HLCS-ai-review.yaml HLCS P50747 NCBITaxon:9606 Homo sapiens 17 R-HSA-2993802 HLCS biotinylates PC:Mn2+ no GO:0004077 biotin--[biotin carboxyl-carrier protein] ligase activity TAS enables MF false ACCEPT "Reactome (TAS) biotin--protein ligase activity for ""HLCS biotinylates PC:Mn2+"" (biotinylation of pyruvate carboxylase). Correct core function on a genuine substrate." reaction_mf_exact accepted_mf uncached_reactome 1 human HLCS genes/human/HLCS/HLCS-ai-review.yaml HLCS P50747 NCBITaxon:9606 Homo sapiens 18 R-HSA-2993814 HLCS biotinylates ACACA:Mn2+ yes reactome/R-HSA-2993814.md HLCS biotinylates ACACA:Mn2+ Homo sapiens GO:0004077 biotin--[biotin carboxyl-carrier protein] ligase activity TAS enables MF false ACCEPT "Reactome (TAS) biotin--protein ligase activity for ""HLCS biotinylates ACACA:Mn2+"" (biotinylation of acetyl-CoA carboxylase 1). Correct core function on a genuine substrate." reaction_mf_exact accepted_mf 1 human HLCS genes/human/HLCS/HLCS-ai-review.yaml HLCS P50747 NCBITaxon:9606 Homo sapiens 19 R-HSA-3323184 Defective HLCS does not biotinylate ACACA:Mn2+ yes reactome/R-HSA-3323184.md Defective HLCS does not biotinylate ACACA:Mn2+ Homo sapiens GO:0004077 biotin--[biotin carboxyl-carrier protein] ligase activity TAS enables MF false ACCEPT "Reactome (TAS) biotin--protein ligase activity within the disease reaction ""Defective HLCS does not biotinylate ACACA:Mn2+"". The annotated activity is the same core ligase function (in a defective-variant context). Correct." reaction_mf_exact accepted_mf reactome_disease_event 1 human HLCS genes/human/HLCS/HLCS-ai-review.yaml HLCS P50747 NCBITaxon:9606 Homo sapiens 20 R-HSA-4167511 HLCS biotinylates ACACB yes reactome/R-HSA-4167511.md HLCS biotinylates ACACB Homo sapiens GO:0004077 biotin--[biotin carboxyl-carrier protein] ligase activity TAS enables MF false ACCEPT "Reactome (TAS) biotin--protein ligase activity for ""HLCS biotinylates ACACB"" (biotinylation of acetyl-CoA carboxylase 2). Correct core function on a genuine substrate." reaction_mf_exact accepted_mf 1 human HLCS genes/human/HLCS/HLCS-ai-review.yaml HLCS P50747 NCBITaxon:9606 Homo sapiens 21 R-HSA-9035987 Defective HLCS does not biotinylate 6xMCCC1:6xMCCC2 no GO:0004077 biotin--[biotin carboxyl-carrier protein] ligase activity TAS enables MF false ACCEPT "Reactome (TAS) biotin--protein ligase activity within the disease reaction ""Defective HLCS does not biotinylate 6xMCCC1:6xMCCC2"". Same core ligase function in a defective-variant context. Correct." reaction_mf_exact accepted_mf reactome_disease_event|uncached_reactome 1 human HLCS genes/human/HLCS/HLCS-ai-review.yaml HLCS P50747 NCBITaxon:9606 Homo sapiens 22 R-HSA-9035988 Defective HLCS does not biotinylate PC:Mn2+ no GO:0004077 biotin--[biotin carboxyl-carrier protein] ligase activity TAS enables MF false ACCEPT "Reactome (TAS) biotin--protein ligase activity within the disease reaction ""Defective HLCS does not biotinylate PC:Mn2+"". Same core ligase function in a defective-variant context. Correct." reaction_mf_exact accepted_mf reactome_disease_event|uncached_reactome 1 human HLCS genes/human/HLCS/HLCS-ai-review.yaml HLCS P50747 NCBITaxon:9606 Homo sapiens 23 R-HSA-9035990 Defective HLCS does not biotinylate 6x(PCCA:PCCB) no GO:0004077 biotin--[biotin carboxyl-carrier protein] ligase activity TAS enables MF false ACCEPT "Reactome (TAS) biotin--protein ligase activity within the disease reaction ""Defective HLCS does not biotinylate 6x(PCCA:PCCB)"". Same core ligase function in a defective-variant context. Correct." reaction_mf_exact accepted_mf reactome_disease_event|uncached_reactome 1 human HMBS genes/human/HMBS/HMBS-ai-review.yaml HMBS P08397 NCBITaxon:9606 Homo sapiens 12 R-HSA-189406 4 PBGs bind to form HMB yes reactome/R-HSA-189406.md 4 PBGs bind to form HMB Homo sapiens GO:0004418 hydroxymethylbilane synthase activity TAS enables MF false ACCEPT "Reactome TAS annotation (reaction ""4 PBGs bind to form HMB"") of hydroxymethylbilane synthase activity. Core catalytic function." reaction_mf_exact accepted_mf 1 human HMGCS1 genes/human/HMGCS1/HMGCS1-ai-review.yaml HMGCS1 Q01581 NCBITaxon:9606 Homo sapiens 11 R-HSA-191323 HMGCS1 condenses Ac-CoA and ACA-CoA to form bHMG-CoA yes reactome/R-HSA-191323.md HMGCS1 condenses Ac-CoA and ACA-CoA to form bHMG-CoA Homo sapiens GO:0004421 hydroxymethylglutaryl-CoA synthase activity TAS enables MF false ACCEPT Reactome traceable assertion of the core HMG-CoA synthase reaction (condensation of acetyl-CoA and acetoacetyl-CoA to HMG-CoA). reaction_mf_exact accepted_mf local_module_mf 1 mevalonate_pathway:GO:0004421 hydroxymethylglutaryl-CoA synthase activity human HMGCS2 genes/human/HMGCS2/HMGCS2-ai-review.yaml HMGCS2 P54868 NCBITaxon:9606 Homo sapiens 43 R-HSA-73918 acetoacetyl-CoA+acetyl-CoA => HMG-CoA + CoASH no GO:0004421 hydroxymethylglutaryl-CoA synthase activity TAS enables MF false ACCEPT Reactome TAS for the HMG-CoA synthase reaction (acetoacetyl-CoA + acetyl-CoA => HMG-CoA + CoASH). Core molecular function. reaction_mf_exact accepted_mf local_module_mf|uncached_reactome 1 ketogenesis:GO:0004421 hydroxymethylglutaryl-CoA synthase activity human HRAS genes/human/HRAS/HRAS-ai-review.yaml HRAS P01112 NCBITaxon:9606 Homo sapiens 84 R-HSA-9649736 TODO: Fetch title yes reactome/R-HSA-9649736.md RAS intrinsic GTPase activity hydrolyzes GTP to GDP Homo sapiens GO:0003924 GTPase activity TAS enables MF false ACCEPT HRAS has intrinsic GTPase activity. reaction_mf_exact accepted_mf multi_gene_reactome_id 3 human HSD17B3 genes/human/HSD17B3/HSD17B3-ai-review.yaml HSD17B3 P37058 NCBITaxon:9606 Homo sapiens 14 R-HSA-193064 HSD17B3-like proteins reducde ANDST to TEST yes reactome/R-HSA-193064.md HSD17B3-like proteins reducde ANDST to TEST Homo sapiens GO:0047045 testosterone dehydrogenase (NADP+) activity TAS enables MF false ACCEPT TAS (Reactome, HSD17B3-like proteins reduce ANDST to TEST) assignment of testosterone dehydrogenase (NADP+) activity, duplicating the core molecular function. reaction_mf_exact accepted_mf local_module_mf 1 sex_steroid_biosynthesis:GO:0047045 testosterone dehydrogenase (NADP+) activity human HSD3B7 genes/human/HSD3B7/HSD3B7-ai-review.yaml HSD3B7 Q9H2F3 NCBITaxon:9606 Homo sapiens 12 R-HSA-192097 7alpha-hydroxycholesterol is oxidized and isomerized to 4-cholesten-7alpha-ol-3-one yes reactome/R-HSA-192097.md 7alpha-hydroxycholesterol is oxidized and isomerized to 4-cholesten-7alpha-ol-3-one Homo sapiens GO:0003854 3-beta-hydroxy-Delta5-steroid dehydrogenase (NAD+) activity TAS enables MF false ACCEPT Core molecular function - NAD+-dependent 3-beta-hydroxy-Delta5-steroid dehydrogenase (with associated isomerase activity). Reactome R-HSA-192097 describes HSD3B7 oxidizing/isomerizing 7-alpha-hydroxycholesterol to 4-cholesten-7-alpha-ol-3-one. reaction_mf_exact accepted_mf local_module_mf 1 bile_acid_synthesis_ring_modification:GO:0003854 3-beta-hydroxy-Delta5-steroid dehydrogenase (NAD+) activity human HSD3B7 genes/human/HSD3B7/HSD3B7-ai-review.yaml HSD3B7 Q9H2F3 NCBITaxon:9606 Homo sapiens 13 R-HSA-193789 Cholest-5-ene-3beta,7alpha,24(S)-triol is oxidized and isomerized to 4-cholesten-7alpha,24(S)-diol-3-one yes reactome/R-HSA-193789.md Cholest-5-ene-3beta,7alpha,24(S)-triol is oxidized and isomerized to 4-cholesten-7alpha,24(S)-diol-3-one Homo sapiens GO:0003854 3-beta-hydroxy-Delta5-steroid dehydrogenase (NAD+) activity TAS enables MF false ACCEPT Same core 3-beta-hydroxy-Delta5-steroid dehydrogenase activity, annotated from the Reactome reaction on cholest-5-ene-3beta,7alpha,24(S)-triol (the 24-hydroxy branch substrate). reaction_mf_exact accepted_mf 1 human HSD3B7 genes/human/HSD3B7/HSD3B7-ai-review.yaml HSD3B7 Q9H2F3 NCBITaxon:9606 Homo sapiens 14 R-HSA-193816 Cholest-5-ene-3beta,7alpha,27-triol is oxidized and isomerized to 4-cholesten-7alpha,27-diol-3-one yes reactome/R-HSA-193816.md Cholest-5-ene-3beta,7alpha,27-triol is oxidized and isomerized to 4-cholesten-7alpha,27-diol-3-one Homo sapiens GO:0003854 3-beta-hydroxy-Delta5-steroid dehydrogenase (NAD+) activity TAS enables MF false ACCEPT Same core 3-beta-hydroxy-Delta5-steroid dehydrogenase activity, annotated from the Reactome reaction on cholest-5-ene-3beta,7alpha,27-triol (the 27-hydroxy branch substrate). reaction_mf_exact accepted_mf 1 human HSPA1A genes/human/HSPA1A/HSPA1A-ai-review.yaml HSPA1A P0DMV8 NCBITaxon:9606 Homo sapiens 60 R-HSA-3371422 ATP hydrolysis by HSP70 yes reactome/R-HSA-3371422.md ATP hydrolysis by HSP70 Homo sapiens GO:0016887 ATP hydrolysis activity TAS enables MF false ACCEPT ATP hydrolysis by HSP70 is a Reactome-curated reaction (R-HSA-3371422). This is the core enzymatic activity of HSPA1A, confirmed by IDA (PMID:21231916) and IBA evidence. reaction_mf_exact accepted_mf multi_gene_reactome_id 3 human HSPA1B genes/human/HSPA1B/HSPA1B-ai-review.yaml HSPA1B P0DMV9 NCBITaxon:9606 Homo sapiens 55 R-HSA-3371422 ATP hydrolysis by HSP70 yes reactome/R-HSA-3371422.md ATP hydrolysis by HSP70 Homo sapiens GO:0016887 ATP hydrolysis activity TAS enables MF false ACCEPT TAS from Reactome entry R-HSA-3371422 describing ATP hydrolysis by HSP70. Core molecular function. reaction_mf_exact accepted_mf multi_gene_reactome_id 3 human HSPA8 genes/human/HSPA8/HSPA8-ai-review.yaml HSPA8 P11142 NCBITaxon:9606 Homo sapiens 107 R-HSA-3371422 ATP hydrolysis by HSP70 yes reactome/R-HSA-3371422.md ATP hydrolysis by HSP70 Homo sapiens GO:0016887 ATP hydrolysis activity TAS enables MF false ACCEPT ATP hydrolysis is the core enzymatic activity of HSPA8, driving the chaperone cycle. Reactome pathway confirms this. reaction_mf_exact accepted_mf multi_gene_reactome_id 3 human HSPA8 genes/human/HSPA8/HSPA8-ai-review.yaml HSPA8 P11142 NCBITaxon:9606 Homo sapiens 108 R-HSA-8868658 HSPA8-mediated ATP hydrolysis promotes vesicle uncoating yes reactome/R-HSA-8868658.md HSPA8-mediated ATP hydrolysis promotes vesicle uncoating Homo sapiens GO:0016887 ATP hydrolysis activity TAS enables MF false ACCEPT ATP hydrolysis is the core enzymatic activity of HSPA8, driving the chaperone cycle. Reactome pathway confirms this. reaction_mf_exact accepted_mf 1 human IDUA genes/human/IDUA/IDUA-ai-review.yaml IDUA P35475 NCBITaxon:9606 Homo sapiens 14 R-HSA-1678716 IDUA cleaves iduronate from HS chain yes reactome/R-HSA-1678716.md IDUA cleaves iduronate from HS chain Homo sapiens GO:0003940 L-iduronidase activity TAS enables MF false ACCEPT Reactome traceable-author assertion of L-iduronidase activity (cleavage of terminal L-iduronate from HS chains). Core molecular function. reaction_mf_exact accepted_mf local_module_mf 1 heparan_sulfate_lysosomal_degradation:GO:0003940 L-iduronidase activity human IDUA genes/human/IDUA/IDUA-ai-review.yaml IDUA P35475 NCBITaxon:9606 Homo sapiens 15 R-HSA-1793186 IDUA hydrolyses the unsulfated alpha-L-iduronosidic link in DS yes reactome/R-HSA-1793186.md IDUA hydrolyses the unsulfated alpha-L-iduronosidic link in DS Homo sapiens GO:0003940 L-iduronidase activity TAS enables MF false ACCEPT Reactome traceable-author assertion of L-iduronidase activity (hydrolysis of the unsulfated alpha-L-iduronosidic link in DS). Core molecular function. reaction_mf_exact accepted_mf 1 human IDUA genes/human/IDUA/IDUA-ai-review.yaml IDUA P35475 NCBITaxon:9606 Homo sapiens 16 R-HSA-2090037 IDUA hydrolyses Heparan sulfate chain(6) yes reactome/R-HSA-2090037.md IDUA hydrolyses Heparan sulfate chain(6) Homo sapiens GO:0003940 L-iduronidase activity TAS enables MF false ACCEPT Reactome traceable-author assertion of L-iduronidase activity within a heparan sulfate chain hydrolysis reaction. Core molecular function. reaction_mf_exact accepted_mf 1 human IDUA genes/human/IDUA/IDUA-ai-review.yaml IDUA P35475 NCBITaxon:9606 Homo sapiens 17 R-HSA-2206299 Defective IDUA does not hydrolyse Heparan sulfate chain(6) yes reactome/R-HSA-2206299.md Defective IDUA does not hydrolyse Heparan sulfate chain(6) Homo sapiens GO:0003940 L-iduronidase activity TAS enables MF false ACCEPT Reactome traceable-author assertion of L-iduronidase activity (associated with a defective-IDUA/disease reaction). Core molecular function. reaction_mf_exact accepted_mf reactome_disease_event 1 human IDUA genes/human/IDUA/IDUA-ai-review.yaml IDUA P35475 NCBITaxon:9606 Homo sapiens 18 R-HSA-9036037 Defective IDUA does not hydrolyse Heparan sulfate chain(1) yes reactome/R-HSA-9036037.md Defective IDUA does not hydrolyse Heparan sulfate chain(1) Homo sapiens GO:0003940 L-iduronidase activity TAS enables MF false ACCEPT Reactome traceable-author assertion of L-iduronidase activity within a heparan-sulfate-chain reaction. Core molecular function. reaction_mf_exact accepted_mf reactome_disease_event 1 human IDUA genes/human/IDUA/IDUA-ai-review.yaml IDUA P35475 NCBITaxon:9606 Homo sapiens 19 R-HSA-9036041 Defective IDUA does not hydrolyse the unsulfated alpha-L-iduronosidic link in DS yes reactome/R-HSA-9036041.md Defective IDUA does not hydrolyse the unsulfated alpha-L-iduronosidic link in DS Homo sapiens GO:0003940 L-iduronidase activity TAS enables MF false ACCEPT Reactome traceable-author assertion of L-iduronidase activity (associated with the defective-IDUA dermatan sulfate reaction). Core molecular function. reaction_mf_exact accepted_mf reactome_disease_event 1 human INPP5D genes/human/INPP5D/INPP5D-ai-review.yaml INPP5D Q92835 NCBITaxon:9606 Homo sapiens 29 R-HSA-1675949 PI(3,4,5)P3 is dephosphorylated to PI(3,4)P2 by INPP5[2] at the plasma membrane yes reactome/R-HSA-1675949.md PI(3,4,5)P3 is dephosphorylated to PI(3,4)P2 by INPP5[2] at the plasma membrane Homo sapiens GO:0034485 phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase activity TAS enables MF false ACCEPT SHIP1/INPP5D is a hematopoietic inositol/phosphatidylinositol 5-phosphatase, with core activity toward PtdIns(3,4,5)P3 and Ins(1,3,4,5)P4. reaction_mf_exact accepted_mf 1 human IP6K3 genes/human/IP6K3/IP6K3-ai-review.yaml IP6K3 Q96PC2 NCBITaxon:9606 Homo sapiens 24 R-HSA-1855227 IP6 is phosphorylated to 5-PP-IP5 by IP6K1/3 in the cytosol yes reactome/R-HSA-1855227.md IP6 is phosphorylated to 5-PP-IP5 by IP6K1/3 in the cytosol Homo sapiens GO:0000828 inositol hexakisphosphate kinase activity TAS enables MF false ACCEPT inositol hexakisphosphate kinase activity fits the characterized IP6K3 catalytic activity toward soluble inositol polyphosphate and inositol pyrophosphate substrates. reaction_mf_exact accepted_mf 1 human IP6K3 genes/human/IP6K3/IP6K3-ai-review.yaml IP6K3 Q96PC2 NCBITaxon:9606 Homo sapiens 25 R-HSA-1855194 1-PP-IP5 is phosphorylated to 1,5-(PP)2-IP4 by IP6K1/3 in the cytosol yes reactome/R-HSA-1855194.md 1-PP-IP5 is phosphorylated to 1,5-(PP)2-IP4 by IP6K1/3 in the cytosol Homo sapiens GO:0000829 diphosphoinositol pentakisphosphate kinase activity TAS enables MF false ACCEPT diphosphoinositol pentakisphosphate kinase activity fits the characterized IP6K3 catalytic activity toward soluble inositol polyphosphate and inositol pyrophosphate substrates. reaction_mf_exact accepted_mf 1 human IP6K3 genes/human/IP6K3/IP6K3-ai-review.yaml IP6K3 Q96PC2 NCBITaxon:9606 Homo sapiens 26 R-HSA-1855158 5-PP-IP5 is phosphorylated to 5-PPP-IP5 by IP6K1/3 in the cytosol yes reactome/R-HSA-1855158.md 5-PP-IP5 is phosphorylated to 5-PPP-IP5 by IP6K1/3 in the cytosol Homo sapiens GO:0052836 inositol 5-diphosphate pentakisphosphate 5-kinase activity TAS enables MF false ACCEPT inositol 5-diphosphate pentakisphosphate 5-kinase activity fits the characterized IP6K3 catalytic activity toward soluble inositol polyphosphate and inositol pyrophosphate substrates. reaction_mf_exact accepted_mf 1 human IP6K3 genes/human/IP6K3/IP6K3-ai-review.yaml IP6K3 Q96PC2 NCBITaxon:9606 Homo sapiens 27 R-HSA-1855193 1-PP-IP4 is phosphorylated to 1,5-(PP)2-IP3 by IP6K1/3 in the cytosol yes reactome/R-HSA-1855193.md 1-PP-IP4 is phosphorylated to 1,5-(PP)2-IP3 by IP6K1/3 in the cytosol Homo sapiens GO:0052839 diphosphoinositol tetrakisphosphate kinase activity TAS enables MF false ACCEPT diphosphoinositol tetrakisphosphate kinase activity fits the characterized IP6K3 catalytic activity toward soluble inositol polyphosphate and inositol pyrophosphate substrates. reaction_mf_exact accepted_mf 1 human IP6K3 genes/human/IP6K3/IP6K3-ai-review.yaml IP6K3 Q96PC2 NCBITaxon:9606 Homo sapiens 28 R-HSA-1855223 I(1,3,4,5,6)P5 is phosphorylated to 5-PP-IP4 by IP6K1/3 in the cytosol yes reactome/R-HSA-1855223.md I(1,3,4,5,6)P5 is phosphorylated to 5-PP-IP4 by IP6K1/3 in the cytosol Homo sapiens GO:0000827 inositol-1,3,4,5,6-pentakisphosphate kinase activity TAS enables MF false ACCEPT inositol-1,3,4,5,6-pentakisphosphate kinase activity fits the characterized IP6K3 catalytic activity toward soluble inositol polyphosphate and inositol pyrophosphate substrates. reaction_mf_exact accepted_mf 1 human IVD genes/human/IVD/IVD-ai-review.yaml IVD P26440 NCBITaxon:9606 Homo sapiens 14 R-HSA-9914837 IVD mutants don't synthesize beta-methylcrotonyl-CoA no GO:0008470 3-methylbutanoyl-CoA dehydrogenase activity TAS enables MF false ACCEPT Isovaleryl-CoA dehydrogenase activity, from a Reactome pathway describing IVD mutants failing to synthesize beta-methylcrotonyl-CoA. Captures the core catalytic function. reaction_mf_exact accepted_mf uncached_reactome 1 human JAK1 genes/human/JAK1/JAK1-ai-review.yaml JAK1 P23458 NCBITaxon:9606 Homo sapiens 50 R-HSA-1112602 Tyrosine phosphorylation of STAT1, STAT3 by IL6 receptor yes reactome/R-HSA-1112602.md Tyrosine phosphorylation of STAT1, STAT3 by IL6 receptor Homo sapiens GO:0004715 non-membrane spanning protein tyrosine kinase activity TAS enables MF false ACCEPT Duplicate Reactome annotation - IL6 receptor signaling reaction_mf_exact accepted_mf 1 human JAK1 genes/human/JAK1/JAK1-ai-review.yaml JAK1 P23458 NCBITaxon:9606 Homo sapiens 51 R-HSA-1112703 PTPN11 is phosphorylated yes reactome/R-HSA-1112703.md PTPN11 is phosphorylated Homo sapiens GO:0004715 non-membrane spanning protein tyrosine kinase activity TAS enables MF false ACCEPT Duplicate Reactome annotation - PTPN11 phosphorylation reaction_mf_exact accepted_mf 1 human JAK1 genes/human/JAK1/JAK1-ai-review.yaml JAK1 P23458 NCBITaxon:9606 Homo sapiens 52 R-HSA-6786095 JAK1 phosphorylates STAT3,STAT6 yes reactome/R-HSA-6786095.md JAK1 phosphorylates STAT3,STAT6 Homo sapiens GO:0004715 non-membrane spanning protein tyrosine kinase activity TAS enables MF false ACCEPT Duplicate Reactome annotation - STAT3/STAT6 phosphorylation reaction_mf_exact accepted_mf 1 human JAK1 genes/human/JAK1/JAK1-ai-review.yaml JAK1 P23458 NCBITaxon:9606 Homo sapiens 53 R-HSA-6786096 IL4R, IL2RG, JAK1 in IL4-bound IL4R1:JAK1 are phosphorylated yes reactome/R-HSA-6786096.md IL4R, IL2RG, JAK1 in IL4-bound IL4R1:JAK1 are phosphorylated Homo sapiens GO:0004715 non-membrane spanning protein tyrosine kinase activity TAS enables MF false ACCEPT Duplicate Reactome annotation - IL4R phosphorylation reaction_mf_exact accepted_mf 1 human JAK1 genes/human/JAK1/JAK1-ai-review.yaml JAK1 P23458 NCBITaxon:9606 Homo sapiens 57 R-HSA-452122 JAK1 phosphorylates Y338, Y392 and Y510 of IL2RB yes reactome/R-HSA-452122.md JAK1 phosphorylates Y338, Y392 and Y510 of IL2RB Homo sapiens GO:0004713 protein tyrosine kinase activity TAS enables MF false ACCEPT Reactome annotation - IL2RB phosphorylation reaction_mf_exact accepted_mf 1 human JAK1 genes/human/JAK1/JAK1-ai-review.yaml JAK1 P23458 NCBITaxon:9606 Homo sapiens 58 R-HSA-873924 Phosphorylation of IFNGR1 by JAK kinases yes reactome/R-HSA-873924.md Phosphorylation of IFNGR1 by JAK kinases Homo sapiens GO:0004713 protein tyrosine kinase activity TAS enables MF false ACCEPT Reactome annotation - IFNGR1 phosphorylation reaction_mf_exact accepted_mf 1 human KDSR genes/human/KDSR/KDSR-ai-review.yaml KDSR Q06136 NCBITaxon:9606 Homo sapiens 12 R-HSA-428123 KDSR reduces 3-ketosphingoid yes reactome/R-HSA-428123.md KDSR reduces 3-ketosphingoid Homo sapiens GO:0047560 3-dehydrosphinganine reductase activity TAS enables MF false ACCEPT "Reactome traceable annotation of the KDS reductase molecular function (reaction ""KDSR reduces 3-ketosphingoid"")." reaction_mf_exact accepted_mf local_module_mf 1 sphingolipid_de_novo_synthesis:GO:0047560 3-dehydrosphinganine reductase activity human KIT genes/human/KIT/KIT-ai-review.yaml KIT P10721 NCBITaxon:9606 Homo sapiens 15 R-HSA-9669890 Constitutive phosphorylation of kinase domain KIT mutants yes reactome/R-HSA-9669890.md Constitutive phosphorylation of kinase domain KIT mutants Homo sapiens GO:0004713 protein tyrosine kinase activity TAS enables MF false ACCEPT KIT is a protein tyrosine kinase (EC 2.7.10.1); accurate core molecular function. reaction_mf_exact accepted_mf 1 human KMO genes/human/KMO/KMO-ai-review.yaml KMO O15229 NCBITaxon:9606 Homo sapiens 22 R-HSA-71200 kynurenine + O2 + NADPH + H+ => 3-hydroxykynurenine + NADP+ + H2O yes reactome/R-HSA-71200.md kynurenine + O2 + NADPH + H+ => 3-hydroxykynurenine + NADP+ + H2O Homo sapiens GO:0004502 kynurenine 3-monooxygenase activity TAS enables MF false ACCEPT Traceable-author (Reactome) statement of the kynurenine 3-monooxygenase reaction (L-kynurenine + NADPH + O2 -> 3-hydroxykynurenine + NADP+ + H2O). This is the core catalytic function. Accept. reaction_mf_exact accepted_mf 1 human KRAS genes/human/KRAS/KRAS-ai-review.yaml KRAS P01116 NCBITaxon:9606 Homo sapiens 50 R-HSA-9649736 RAS intrinsic GTPase activity hydrolyzes GTP to GDP yes reactome/R-HSA-9649736.md RAS intrinsic GTPase activity hydrolyzes GTP to GDP Homo sapiens GO:0003924 GTPase activity TAS enables MF false ACCEPT TAS annotation for KRAS GTPase activity. KRAS is a Ras-family small GTPase with intrinsic GTP hydrolysis activity and GAP-stimulated hydrolysis. reaction_mf_exact accepted_mf multi_gene_reactome_id 3 human LONP1 genes/human/LONP1/LONP1-ai-review.yaml LONP1 P36776 NCBITaxon:9606 Homo sapiens 41 R-HSA-9838004 LONP1 degrades mitochondrial inner membrane proteins yes reactome/R-HSA-9838004.md LONP1 degrades mitochondrial inner membrane proteins Homo sapiens GO:0004176 ATP-dependent peptidase activity TAS enables MF false ACCEPT Correct core catalytic activity in a Reactome substrate-degradation reaction. reaction_mf_exact accepted_mf 1 human LONP1 genes/human/LONP1/LONP1-ai-review.yaml LONP1 P36776 NCBITaxon:9606 Homo sapiens 42 R-HSA-9838081 LONP1 degrades mitochondrial matrix proteins yes reactome/R-HSA-9838081.md LONP1 degrades mitochondrial matrix proteins Homo sapiens GO:0004176 ATP-dependent peptidase activity TAS enables MF false ACCEPT Correct core catalytic activity in a Reactome matrix-protein degradation reaction. reaction_mf_exact accepted_mf 1 human LPCAT1 genes/human/LPCAT1/LPCAT1-ai-review.yaml LPCAT1 Q8NF37 NCBITaxon:9606 Homo sapiens 8 R-HSA-1482539 1-acyl LPG is acylated to PG by LPGAT yes reactome/R-HSA-1482539.md 1-acyl LPG is acylated to PG by LPGAT Homo sapiens GO:0003841 1-acylglycerol-3-phosphate O-acyltransferase activity TAS enables MF false ACCEPT TAS annotation from Reactome for LPGAT activity (1-acyl LPG to PG). This represents LPCAT1's activity on lysophosphatidylglycerol. reaction_mf_exact accepted_mf multi_gene_reactome_id 3 human LPCAT1 genes/human/LPCAT1/LPCAT1-ai-review.yaml LPCAT1 Q8NF37 NCBITaxon:9606 Homo sapiens 9 R-HSA-1482547 1-acyl LPC is acylated to PC by LPCAT yes reactome/R-HSA-1482547.md 1-acyl LPC is acylated to PC by LPCAT Homo sapiens GO:0003841 1-acylglycerol-3-phosphate O-acyltransferase activity TAS enables MF false ACCEPT TAS annotation from Reactome pathway for 1-acyl LPC acylation to PC. reaction_mf_exact accepted_mf multi_gene_reactome_id 4 human LPCAT1 genes/human/LPCAT1/LPCAT1-ai-review.yaml LPCAT1 Q8NF37 NCBITaxon:9606 Homo sapiens 10 R-HSA-75885 1-acyl LPA is acylated to PA by AGPAT (LPAAT) yes reactome/R-HSA-75885.md 1-acyl LPA is acylated to PA by AGPAT (LPAAT) Homo sapiens GO:0003841 1-acylglycerol-3-phosphate O-acyltransferase activity TAS enables MF false ACCEPT TAS annotation for PA synthesis (1-acyl LPA to PA). Part of the Reactome phospholipid biosynthesis pathway. reaction_mf_exact accepted_mf multi_gene_reactome_id 3 human LPCAT1 genes/human/LPCAT1/LPCAT1-ai-review.yaml LPCAT1 Q8NF37 NCBITaxon:9606 Homo sapiens 12 R-HSA-1482533 2-acyl LPC is acylated to PC by LPCAT yes reactome/R-HSA-1482533.md 2-acyl LPC is acylated to PC by LPCAT Homo sapiens GO:0047144 2-acylglycerol-3-phosphate O-acyltransferase activity TAS enables MF false ACCEPT TAS annotation for 2-acyl LPC acylation to PC. This represents activity on 2-acyl-substituted lysophospholipids, which are less common than 1-acyl species. reaction_mf_exact accepted_mf multi_gene_reactome_id 4 human LPCAT1 genes/human/LPCAT1/LPCAT1-ai-review.yaml LPCAT1 Q8NF37 NCBITaxon:9606 Homo sapiens 13 R-HSA-1482635 2-acyl LPG is acylated to PG by LPGAT yes reactome/R-HSA-1482635.md 2-acyl LPG is acylated to PG by LPGAT Homo sapiens GO:0047144 2-acylglycerol-3-phosphate O-acyltransferase activity TAS enables MF false ACCEPT TAS annotation for 2-acyl LPG acylation to PG. reaction_mf_exact accepted_mf multi_gene_reactome_id 3 human LPCAT2 genes/human/LPCAT2/LPCAT2-ai-review.yaml LPCAT2 Q7L5N7 NCBITaxon:9606 Homo sapiens 12 R-HSA-1482547 1-acyl LPC is acylated to PC by LPCAT yes reactome/R-HSA-1482547.md 1-acyl LPC is acylated to PC by LPCAT Homo sapiens GO:0003841 1-acylglycerol-3-phosphate O-acyltransferase activity TAS enables MF false ACCEPT Reactome annotation for AGPAT activity is consistent with experimental evidence demonstrating LPCAT2's ability to acylate LPA at sn-1. reaction_mf_exact accepted_mf multi_gene_reactome_id 4 human LRAT genes/human/LRAT/LRAT-ai-review.yaml LRAT O95237 NCBITaxon:9606 Homo sapiens 22 R-HSA-975608 LRAT esterifies RBP2:atROL and FACYLs to atREs yes reactome/R-HSA-975608.md LRAT esterifies RBP2:atROL and FACYLs to atREs Homo sapiens GO:0047173 phosphatidylcholine-retinol O-acyltransferase activity TAS enables MF false ACCEPT "Reactome TAS annotation (reaction ""LRAT esterifies RBP2:atROL and FACYLs to atREs"") to the precise molecular function phosphatidylcholine-retinol O-acyltransferase activity. This matches the enzyme's core catalytic function and the experimental IDA. Core function." reaction_mf_exact accepted_mf 1 human LRP1 genes/human/LRP1/LRP1-ai-review.yaml LRP1 Q07954 NCBITaxon:9606 Homo sapiens 51 R-HSA-2404131 LRPs transport extracellular CR:atREs:HSPG:apoE to cytosol yes reactome/R-HSA-2404131.md LRPs transport extracellular CR:atREs:HSPG:apoE to cytosol Homo sapiens GO:0005041 low-density lipoprotein particle receptor activity TAS enables MF false ACCEPT low-density lipoprotein particle receptor activity is retained as a core molecular-function annotation for LRP1; it captures activity or binding specificity within the synthesized core biology: LRP1 cell-surface/coated-pit/endosomal receptor biology, ligand and calcium binding, apolipoprotein/lipoprotein and alpha-2-macroglobulin cargo recognition, receptor-mediated endocytosis, transcytosis, phagocytic/lysosomal clearance, and Alzheimer-relevant amyloid-beta/tau handling. reaction_mf_exact accepted_mf multi_gene_reactome_id 3 human LRP2 genes/human/LRP2/LRP2-ai-review.yaml LRP2 P98164 NCBITaxon:9606 Homo sapiens 56 R-HSA-350168 LRP2-mediated uptake of extracellular CUBN:GC:25(OH)D yes reactome/R-HSA-350168.md LRP2-mediated uptake of extracellular CUBN:GC:25(OH)D Homo sapiens GO:0038024 cargo receptor activity TAS enables MF false ACCEPT Reactome:R-HSA-350168 provides statement-level support for the LRP2 annotation to cargo receptor activity. reaction_mf_exact accepted_mf 1 human LSS genes/human/LSS/LSS-ai-review.yaml LSS P48449 NCBITaxon:9606 Homo sapiens 12 R-HSA-191366 Squalene 2,3-epoxide cyclizes, forming lanosterol yes reactome/R-HSA-191366.md Squalene 2,3-epoxide cyclizes, forming lanosterol Homo sapiens GO:0000250 lanosterol synthase activity TAS enables MF false ACCEPT Reactome traceable assertion of the core catalytic function: LSS cyclizes squalene 2,3-epoxide to lanosterol. reaction_mf_exact accepted_mf local_module_mf 1 sterol_commitment_squalene_to_lanosterol:GO:0000250 lanosterol synthase activity human LTN1 genes/human/LTN1/LTN1-ai-review.yaml LTN1 O94822 NCBITaxon:9606 Homo sapiens 17 R-HSA-9948362 Reactome: LTN1 ubiquitin ligase activity yes reactome/R-HSA-9948362.md LTN1 K48 polyubiquitinates the nascent peptide in NEMF:60S ribosomal subunit:peptidyl-tRNA with nascent peptide Homo sapiens GO:0061630 ubiquitin protein ligase activity TAS enables MF false ACCEPT Reactome curation of the core RING E3 ligase activity of LTN1, consistent with experimental evidence. reaction_mf_exact accepted_mf 1 human LYN genes/human/LYN/LYN-ai-review.yaml LYN P07948 NCBITaxon:9606 Homo sapiens 42 R-HSA-1433506 Phosphorylation of APS yes reactome/R-HSA-1433506.md Phosphorylation of APS Homo sapiens GO:0004713 protein tyrosine kinase activity TAS enables MF false ACCEPT Protein tyrosine kinase activity - a core, experimentally established molecular function of LYN. reaction_mf_exact accepted_mf 1 human LYN genes/human/LYN/LYN-ai-review.yaml LYN P07948 NCBITaxon:9606 Homo sapiens 43 R-HSA-1472121 Phosphorylation of p-KIT on Y900 by Src kinases yes reactome/R-HSA-1472121.md Phosphorylation of p-KIT on Y900 by Src kinases Homo sapiens GO:0004713 protein tyrosine kinase activity TAS enables MF false ACCEPT Protein tyrosine kinase activity - a core, experimentally established molecular function of LYN. reaction_mf_exact accepted_mf 1 human LYN genes/human/LYN/LYN-ai-review.yaml LYN P07948 NCBITaxon:9606 Homo sapiens 44 R-HSA-210291 Phosphorylation of PECAM-1 by Fyn or Lyn or c-Src yes reactome/R-HSA-210291.md Phosphorylation of PECAM-1 by Fyn or Lyn or c-Src Homo sapiens GO:0004713 protein tyrosine kinase activity TAS enables MF false ACCEPT Protein tyrosine kinase activity - a core, experimentally established molecular function of LYN. reaction_mf_exact accepted_mf 1 human LYN genes/human/LYN/LYN-ai-review.yaml LYN P07948 NCBITaxon:9606 Homo sapiens 45 R-HSA-2730862 Autophosphorylation of LYN kinase yes reactome/R-HSA-2730862.md Autophosphorylation of LYN kinase Homo sapiens GO:0004713 protein tyrosine kinase activity TAS enables MF false ACCEPT Protein tyrosine kinase activity - a core, experimentally established molecular function of LYN. reaction_mf_exact accepted_mf 1 human LYN genes/human/LYN/LYN-ai-review.yaml LYN P07948 NCBITaxon:9606 Homo sapiens 46 R-HSA-388831 Phosphorylation of CD28 yes reactome/R-HSA-388831.md Phosphorylation of CD28 Homo sapiens GO:0004713 protein tyrosine kinase activity TAS enables MF false ACCEPT Protein tyrosine kinase activity - a core, experimentally established molecular function of LYN. reaction_mf_exact accepted_mf 1 human LYN genes/human/LYN/LYN-ai-review.yaml LYN P07948 NCBITaxon:9606 Homo sapiens 47 R-HSA-388833 Phosphorylation of CTLA-4 yes reactome/R-HSA-388833.md Phosphorylation of CTLA-4 Homo sapiens GO:0004713 protein tyrosine kinase activity TAS enables MF false ACCEPT Protein tyrosine kinase activity - a core, experimentally established molecular function of LYN. reaction_mf_exact accepted_mf 1 human LYN genes/human/LYN/LYN-ai-review.yaml LYN P07948 NCBITaxon:9606 Homo sapiens 48 R-HSA-5624486 SFKs phosphorylates RAF1 on Y340,Y341 yes reactome/R-HSA-5624486.md SFKs phosphorylates RAF1 on Y340,Y341 Homo sapiens GO:0004713 protein tyrosine kinase activity TAS enables MF false ACCEPT Protein tyrosine kinase activity - a core, experimentally established molecular function of LYN. reaction_mf_exact accepted_mf 1 human LYN genes/human/LYN/LYN-ai-review.yaml LYN P07948 NCBITaxon:9606 Homo sapiens 49 R-HSA-5690702 LYN phosphorylates CD22 yes reactome/R-HSA-5690702.md LYN phosphorylates CD22 Homo sapiens GO:0004713 protein tyrosine kinase activity TAS enables MF false ACCEPT Protein tyrosine kinase activity - a core, experimentally established molecular function of LYN. reaction_mf_exact accepted_mf 1 human LYN genes/human/LYN/LYN-ai-review.yaml LYN P07948 NCBITaxon:9606 Homo sapiens 50 R-HSA-9664261 Src phosphorylate SYK in IgG:Leishmania surface:p-FCGR3A:SYK yes reactome/R-HSA-9664261.md Src phosphorylate SYK in IgG:Leishmania surface:p-FCGR3A:SYK Homo sapiens GO:0004713 protein tyrosine kinase activity TAS enables MF false ACCEPT Protein tyrosine kinase activity - a core, experimentally established molecular function of LYN. reaction_mf_exact accepted_mf 1 human LYN genes/human/LYN/LYN-ai-review.yaml LYN P07948 NCBITaxon:9606 Homo sapiens 54 R-HSA-1168459 Lyn activates ERK yes reactome/R-HSA-1168459.md Lyn activates ERK Homo sapiens GO:0004715 non-membrane spanning protein tyrosine kinase activity TAS enables MF false ACCEPT Non-membrane spanning protein tyrosine kinase activity - the single most precise molecular-function term for LYN. reaction_mf_exact accepted_mf 1 human LYRM7 genes/human/LYRM7/LYRM7-ai-review.yaml LYRM7 Q5U5X0 NCBITaxon:9606 Homo sapiens 16 R-HSA-9866253 apo-UQCRFS1 binds LYRM7 yes reactome/R-HSA-9866253.md apo-UQCRFS1 binds LYRM7 Homo sapiens GO:0044183 protein folding chaperone TAS enables MF false ACCEPT Reactome (TAS) assignment of the protein folding chaperone MF, corresponding to the reaction in which apo-UQCRFS1 binds and is stabilised by LYRM7. Matches the core chaperone function. reaction_mf_exact accepted_mf local_module_mf 1 mitochondrial_complex_iii:GO:0044183 protein folding chaperone human MAN1B1 genes/human/MAN1B1/MAN1B1-ai-review.yaml MAN1B1 Q9UKM7 NCBITaxon:9606 Homo sapiens 12 R-HSA-4793949 Defective MAN1B1 does not hydrolyse 1,2-linked mannose (a branch) yes reactome/R-HSA-4793949.md Defective MAN1B1 does not hydrolyse 1,2-linked mannose (a branch) Homo sapiens GO:0004571 mannosyl-oligosaccharide 1,2-alpha-mannosidase activity TAS enables MF false ACCEPT Reactome curation of MAN1B1 mannosidase activity (defective-MAN1B1 reaction context). reaction_mf_exact accepted_mf reactome_disease_event 1 human MAN1B1 genes/human/MAN1B1/MAN1B1-ai-review.yaml MAN1B1 Q9UKM7 NCBITaxon:9606 Homo sapiens 13 R-HSA-901024 MAN1B1 hydrolyses 1,2-linked mannose (a branch) yes reactome/R-HSA-901024.md MAN1B1 hydrolyses 1,2-linked mannose (a branch) Homo sapiens GO:0004571 mannosyl-oligosaccharide 1,2-alpha-mannosidase activity TAS enables MF false ACCEPT Reactome curation of MAN1B1 hydrolysis of a 1,2-linked mannose (a branch). reaction_mf_exact accepted_mf multi_gene_reactome_id 2 human MAN1B1 genes/human/MAN1B1/MAN1B1-ai-review.yaml MAN1B1 Q9UKM7 NCBITaxon:9606 Homo sapiens 14 R-HSA-901036 MAN1B1 hydrolyses a second 1,2-linked mannose (a branch) yes reactome/R-HSA-901036.md MAN1B1 hydrolyses a second 1,2-linked mannose (a branch) Homo sapiens GO:0004571 mannosyl-oligosaccharide 1,2-alpha-mannosidase activity TAS enables MF false ACCEPT Reactome curation of MAN1B1 hydrolysis of a second 1,2-linked mannose (a branch). reaction_mf_exact accepted_mf multi_gene_reactome_id 2 human MAN1B1 genes/human/MAN1B1/MAN1B1-ai-review.yaml MAN1B1 Q9UKM7 NCBITaxon:9606 Homo sapiens 15 R-HSA-901039 MAN1B1 hydrolyses 1,2-linked mannose (c branch) yes reactome/R-HSA-901039.md MAN1B1 hydrolyses 1,2-linked mannose (c branch) Homo sapiens GO:0004571 mannosyl-oligosaccharide 1,2-alpha-mannosidase activity TAS enables MF false ACCEPT Reactome curation of MAN1B1 hydrolysis of a 1,2-linked mannose (c branch). reaction_mf_exact accepted_mf multi_gene_reactome_id 2 human MAN1B1 genes/human/MAN1B1/MAN1B1-ai-review.yaml MAN1B1 Q9UKM7 NCBITaxon:9606 Homo sapiens 16 R-HSA-901074 MAN1B1,EDEM2 hydrolyse 1,2-linked mannose (b branch) yes reactome/R-HSA-901074.md MAN1B1,EDEM2 hydrolyse 1,2-linked mannose (b branch) Homo sapiens GO:0004571 mannosyl-oligosaccharide 1,2-alpha-mannosidase activity TAS enables MF false ACCEPT Reactome curation of MAN1B1/EDEM2 hydrolysis of a 1,2-linked mannose (b branch). reaction_mf_exact accepted_mf multi_gene_reactome_id 2 human MAN1B1 genes/human/MAN1B1/MAN1B1-ai-review.yaml MAN1B1 Q9UKM7 NCBITaxon:9606 Homo sapiens 17 R-HSA-9036008 Defective MAN1B1 does not hydrolyse a second 1,2-linked mannose (a branch) yes reactome/R-HSA-9036008.md Defective MAN1B1 does not hydrolyse a second 1,2-linked mannose (a branch) Homo sapiens GO:0004571 mannosyl-oligosaccharide 1,2-alpha-mannosidase activity TAS enables MF false ACCEPT Reactome curation of MAN1B1 mannosidase activity (defective-MAN1B1 reaction context). reaction_mf_exact accepted_mf reactome_disease_event 1 human MAN1B1 genes/human/MAN1B1/MAN1B1-ai-review.yaml MAN1B1 Q9UKM7 NCBITaxon:9606 Homo sapiens 18 R-HSA-9036011 Defective MAN1B1 does not hydrolyse 1,2-linked mannose (b branch) yes reactome/R-HSA-9036011.md Defective MAN1B1 does not hydrolyse 1,2-linked mannose (b branch) Homo sapiens GO:0004571 mannosyl-oligosaccharide 1,2-alpha-mannosidase activity TAS enables MF false ACCEPT Reactome curation of MAN1B1 mannosidase activity (defective-MAN1B1 reaction context). reaction_mf_exact accepted_mf reactome_disease_event 1 human MAN1B1 genes/human/MAN1B1/MAN1B1-ai-review.yaml MAN1B1 Q9UKM7 NCBITaxon:9606 Homo sapiens 19 R-HSA-9036012 Defective MAN1B1 does not hydrolyse 1,2-linked mannose (c branch) yes reactome/R-HSA-9036012.md Defective MAN1B1 does not hydrolyse 1,2-linked mannose (c branch) Homo sapiens GO:0004571 mannosyl-oligosaccharide 1,2-alpha-mannosidase activity TAS enables MF false ACCEPT Reactome curation of MAN1B1 mannosidase activity (defective-MAN1B1 reaction context). reaction_mf_exact accepted_mf reactome_disease_event 1 human MAN1B1 genes/human/MAN1B1/MAN1B1-ai-review.yaml MAN1B1 Q9UKM7 NCBITaxon:9606 Homo sapiens 20 R-HSA-9696807 N-glycan mannose trimming of Spike yes reactome/R-HSA-9696807.md N-glycan mannose trimming of Spike Homo sapiens GO:0004571 mannosyl-oligosaccharide 1,2-alpha-mannosidase activity TAS enables MF false ACCEPT Reactome curation of MAN1B1 mannosidase activity in the context of N-glycan mannose trimming of viral (SARS-CoV-2) spike. reaction_mf_exact accepted_mf multi_gene_reactome_id 2 human MAPK1 genes/human/MAPK1/MAPK1-ai-review.yaml MAPK1 P28482 NCBITaxon:9606 Homo sapiens 106 R-HSA-111898 TODO: Fetch title yes reactome/R-HSA-111898.md Phosphorylation of cPLA2 by ERK-2 Homo sapiens GO:0004707 MAP kinase activity TAS enables MF false ACCEPT MAPK1/ERK2 has MAP kinase activity. reaction_mf_exact accepted_mf 1 human MAPK1 genes/human/MAPK1/MAPK1-ai-review.yaml MAPK1 P28482 NCBITaxon:9606 Homo sapiens 107 R-HSA-445079 TODO: Fetch title yes reactome/R-HSA-445079.md Phosphorylation of L1 by ERK Homo sapiens GO:0004707 MAP kinase activity TAS enables MF false ACCEPT MAPK1/ERK2 has MAP kinase activity. reaction_mf_exact accepted_mf 1 human MASTL genes/human/MASTL/MASTL-ai-review.yaml MASTL Q96GX5 NCBITaxon:9606 Homo sapiens 7 R-HSA-2168079 MASTL (GWL) phosphorylates ARPP19 yes reactome/R-HSA-2168079.md MASTL (GWL) phosphorylates ARPP19 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Reactome reaction in which MASTL phosphorylates ARPP19 on Ser62. reaction_mf_exact accepted_mf 1 human MASTL genes/human/MASTL/MASTL-ai-review.yaml MASTL Q96GX5 NCBITaxon:9606 Homo sapiens 8 R-HSA-2430535 MASTL phosphorylates ENSA yes reactome/R-HSA-2430535.md MASTL phosphorylates ENSA Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Reactome reaction in which MASTL phosphorylates ENSA on Ser67. reaction_mf_exact accepted_mf 1 human MCEE genes/human/MCEE/MCEE-ai-review.yaml MCEE Q96PE7 NCBITaxon:9606 Homo sapiens 10 R-HSA-71020 D-methylmalonyl-CoA <=> L-methylmalonyl-CoA no GO:0004493 methylmalonyl-CoA epimerase activity TAS enables MF false ACCEPT Author-asserted (Reactome) epimerase molecular function. reaction_mf_exact accepted_mf local_module_mf|uncached_reactome 1 propionyl_coa_catabolism:GO:0004493 methylmalonyl-CoA epimerase activity human MGAT1 genes/human/MGAT1/MGAT1-ai-review.yaml MGAT1 P26572 NCBITaxon:9606 Homo sapiens 10 R-HSA-964768 Addition of GlcNAc to the glycan on the A arm yes reactome/R-HSA-964768.md Addition of GlcNAc to the glycan on the A arm Homo sapiens GO:0003827 alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity TAS enables MF false ACCEPT "Reactome traceable annotation for the reaction ""Addition of GlcNAc to the glycan on the A arm"", described as the first committed step in complex/hybrid N-glycan synthesis. Exactly the core GlcNAc-TI activity. Accept." reaction_mf_exact accepted_mf 1 human MKRN1 genes/human/MKRN1/MKRN1-ai-review.yaml MKRN1 Q9UHC7 NCBITaxon:9606 Homo sapiens 9 R-HSA-8948775 Reactome pathway annotation (MKRN1 ubiquitin ligase activity) yes reactome/R-HSA-8948775.md MKRN1 polyubiquitinates PTEN Homo sapiens GO:0061630 ubiquitin protein ligase activity TAS enables MF false ACCEPT Reactome TAS annotation of the core E3 ligase MF. Supported. reaction_mf_exact accepted_mf 1 human MMAB genes/human/MMAB/MMAB-ai-review.yaml MMAB Q96EY8 NCBITaxon:9606 Homo sapiens 13 R-HSA-3322125 Defective MMAB does not transfer adenosyl group from ATP to B12s no GO:0008817 corrinoid adenosyltransferase activity TAS enables MF false ACCEPT Reactome-curated corrinoid adenosyltransferase activity of MMAB, corresponding to the reaction MMAB adenosylates cob(I)alamin. Consistent with all experimental evidence. reaction_mf_exact accepted_mf reactome_disease_event|uncached_reactome 1 human MMACHC genes/human/MMACHC/MMACHC-ai-review.yaml MMACHC Q9Y4U1 NCBITaxon:9606 Homo sapiens 12 R-HSA-3149519 MMACHC decyanates CNCbl no GO:0033787 cyanocobalamin reductase (cyanide-eliminating) (NADP+) activity TAS enables MF false ACCEPT "Reactome TAS for the ""MMACHC decyanates CNCbl"" reaction, annotated to cyanocobalamin reductase activity. Matches the directly demonstrated decyanation function." reaction_mf_exact accepted_mf uncached_reactome 1 human MMADHC genes/human/MMADHC/MMADHC-ai-review.yaml MMADHC Q9H3L0 NCBITaxon:9606 Homo sapiens 10 R-HSA-3149563 MMADHC targets transport of cytosolic cob(II)alamin to mitochondria yes reactome/R-HSA-3149563.md MMADHC targets transport of cytosolic cob(II)alamin to mitochondria Homo sapiens GO:0140104 molecular carrier activity TAS enables MF false ACCEPT Reactome TAS annotation (MMADHC targets transport of cytosolic cob(II)alamin to mitochondria) to molecular carrier activity, capturing MMADHC's cobalamin carrier/routing role. reaction_mf_exact accepted_mf local_module_mf 1 cobalamin_intracellular_processing:GO:0140104 molecular carrier activity human MOCOS genes/human/MOCOS/MOCOS-ai-review.yaml MOCOS Q96EN8 NCBITaxon:9606 Homo sapiens 16 R-HSA-947499 Exchange of oxygen with sulfur in MoCo yes reactome/R-HSA-947499.md Exchange of oxygen with sulfur in MoCo Homo sapiens GO:0008265 molybdenum cofactor sulfurtransferase activity TAS enables MF false ACCEPT Reactome TAS for the MoCo oxygen-to-sulfur exchange reaction, the core sulfurtransferase activity of MOCOS. Accepted; corroborates the experimental annotations to this term. reaction_mf_exact accepted_mf 1 human MOCS2 genes/human/MOCS2/MOCS2-ai-review.yaml MOCS2 O96007 NCBITaxon:9606 Homo sapiens 10 R-HSA-947541 Sulfhydrylation and ring cleavage of precursor Z yes reactome/R-HSA-947541.md Sulfhydrylation and ring cleavage of precursor Z Homo sapiens GO:0030366 molybdopterin synthase activity TAS enables MF false ACCEPT Reactome traceable-author-statement for the core catalytic activity (sulfhydrylation and ring cleavage of precursor Z by the MOCS2B half of the tetramer). Accurate. reaction_mf_exact accepted_mf 1 human MSMO1 genes/human/MSMO1/MSMO1-ai-review.yaml MSMO1 Q15800 NCBITaxon:9606 Homo sapiens 12 R-HSA-194641 4,4-dimethylcholesta-8(9),24-dien-3beta-ol is oxidized to 4-methyl,4-carboxycholesta-8(9),24-dien-3beta-ol yes reactome/R-HSA-194641.md 4,4-dimethylcholesta-8(9),24-dien-3beta-ol is oxidized to 4-methyl,4-carboxycholesta-8(9),24-dien-3beta-ol Homo sapiens GO:0000254 C-4 methylsterol oxidase activity TAS enables MF false ACCEPT Reactome TAS for C-4 methylsterol oxidase activity, describing the SC4MOL-catalyzed oxidation of 4,4-dimethylcholesta-8(9),24-dien-3beta-ol in the ER. reaction_mf_exact accepted_mf local_module_mf 1 cholesterol_synthesis_post_lanosterol:GO:0000254 C-4 methylsterol oxidase activity human MTHFD2 genes/human/MTHFD2/MTHFD2-ai-review.yaml MTHFD2 P13995 NCBITaxon:9606 Homo sapiens 15 R-HSA-6801328 MTHFD2, D2L oxidise 5,10-methylene-THF to 5,10-methenyl-THF yes reactome/R-HSA-6801328.md MTHFD2, D2L oxidise 5,10-methylene-THF to 5,10-methenyl-THF Homo sapiens GO:0004477 methenyltetrahydrofolate cyclohydrolase activity TAS enables MF false ACCEPT Core cyclohydrolase activity, curated by Reactome as the reaction converting 5,10-methenyl-THF to 10-formyl-THF. Accept. reaction_mf_exact accepted_mf 1 human MTHFD2 genes/human/MTHFD2/MTHFD2-ai-review.yaml MTHFD2 P13995 NCBITaxon:9606 Homo sapiens 16 R-HSA-6801462 MTHFD2, D2L oxidise 5,10-methenyl-THF to 10-formyl-THF yes reactome/R-HSA-6801462.md MTHFD2, D2L oxidise 5,10-methenyl-THF to 10-formyl-THF Homo sapiens GO:0004487 methylenetetrahydrofolate dehydrogenase (NAD+) activity TAS enables MF false ACCEPT Core NAD-dependent dehydrogenase activity, curated by Reactome as the oxidation of 5,10-methylene-THF to 5,10-methenyl-THF. Accept. reaction_mf_exact accepted_mf 1 human MTR genes/human/MTR/MTR-ai-review.yaml MTR Q99707 NCBITaxon:9606 Homo sapiens 21 R-HSA-174374 MTR transfers CH3 from MeCbl to HCYS no GO:0008705 methionine synthase activity TAS enables MF false ACCEPT Reactome TAS for methionine synthase activity, describing the half reaction in which the methyl group of methylcobalamin is transferred to homocysteine. Core molecular function. reaction_mf_exact accepted_mf uncached_reactome 1 human MTR genes/human/MTR/MTR-ai-review.yaml MTR Q99707 NCBITaxon:9606 Homo sapiens 22 R-HSA-3149539 MTR transfers CH3 group from 5-methyl-THF to cob(I)alamin no GO:0008705 methionine synthase activity TAS enables MF false ACCEPT Reactome TAS for methionine synthase activity, describing the half reaction in which cob(I)alamin is re-methylated by 5-methyltetrahydrofolate. Core molecular function. reaction_mf_exact accepted_mf local_module_mf|uncached_reactome 1 homocysteine_metabolism:GO:0008705 methionine synthase activity human NAGLU genes/human/NAGLU/NAGLU-ai-review.yaml NAGLU P54802 NCBITaxon:9606 Homo sapiens 9 R-HSA-1678742 NAGLU hydrolyses Heparan sulfate chain(4) yes reactome/R-HSA-1678742.md NAGLU hydrolyses Heparan sulfate chain(4) Homo sapiens GO:0004561 alpha-N-acetylglucosaminidase activity TAS enables MF false ACCEPT "Reactome (TAS) annotation of the core catalytic activity in the reaction ""NAGLU hydrolyses Heparan sulfate chain(4)""." reaction_mf_exact accepted_mf local_module_mf 1 heparan_sulfate_lysosomal_degradation:GO:0004561 alpha-N-acetylglucosaminidase activity human NAGLU genes/human/NAGLU/NAGLU-ai-review.yaml NAGLU P54802 NCBITaxon:9606 Homo sapiens 10 R-HSA-2090038 NAGLU hydrolyses heparan chain(2) yes reactome/R-HSA-2090038.md NAGLU hydrolyses heparan chain(2) Homo sapiens GO:0004561 alpha-N-acetylglucosaminidase activity TAS enables MF false ACCEPT Reactome (TAS) annotation of the core catalytic activity in a second heparan chain hydrolysis reaction. reaction_mf_exact accepted_mf 1 human NAGLU genes/human/NAGLU/NAGLU-ai-review.yaml NAGLU P54802 NCBITaxon:9606 Homo sapiens 11 R-HSA-2263496 Defective NAGLU does not hydrolyse Heparan sulfate chain(4) yes reactome/R-HSA-2263496.md Defective NAGLU does not hydrolyse Heparan sulfate chain(4) Homo sapiens GO:0004561 alpha-N-acetylglucosaminidase activity TAS enables MF false ACCEPT Reactome (TAS) annotation of the core catalytic activity, from a reaction contrasting wild-type with defective NAGLU. reaction_mf_exact accepted_mf reactome_disease_event 1 human NAGLU genes/human/NAGLU/NAGLU-ai-review.yaml NAGLU P54802 NCBITaxon:9606 Homo sapiens 12 R-HSA-9036052 Defective NAGLU does not hydrolyse heparan chain(2) yes reactome/R-HSA-9036052.md Defective NAGLU does not hydrolyse heparan chain(2) Homo sapiens GO:0004561 alpha-N-acetylglucosaminidase activity TAS enables MF false ACCEPT Reactome (TAS) annotation of the core catalytic activity, from a further heparan chain hydrolysis reaction. reaction_mf_exact accepted_mf reactome_disease_event 1 human NAMPT genes/human/NAMPT/NAMPT-ai-review.yaml NAMPT P43490 NCBITaxon:9606 Homo sapiens 16 R-HSA-197250 NAMPT transfers PRIB to NAM to form NAMN yes reactome/R-HSA-197250.md NAMPT transfers PRIB to NAM to form NAMN Homo sapiens GO:0047280 nicotinamide phosphoribosyltransferase activity TAS enables MF false ACCEPT "TAS annotation from the Reactome reaction ""NAMPT transfers PRIB to NAM to form NAMN"" to the core catalytic MF. Correct; corroborates the experimental and IBA annotations. Core function." reaction_mf_exact accepted_mf 1 human NANS genes/human/NANS/NANS-ai-review.yaml NANS Q9NR45 NCBITaxon:9606 Homo sapiens 6 R-HSA-4084976 NANS converts ManNAc-6-P to Neu5Ac-9-P yes reactome/R-HSA-4084976.md NANS converts ManNAc-6-P to Neu5Ac-9-P Homo sapiens GO:0047444 N-acylneuraminate-9-phosphate synthase activity TAS enables MF false ACCEPT Reactome traceable annotation for the NANS reaction (ManNAc-6-P -> Neu5Ac-9-P). Correct core molecular function. reaction_mf_exact accepted_mf 1 human NEU1 genes/human/NEU1/NEU1-ai-review.yaml NEU1 Q99519 NCBITaxon:9606 Homo sapiens 13 R-HSA-4084999 NEU1 hydrolyses Neu5Ac from glycoconjugates yes reactome/R-HSA-4084999.md NEU1 hydrolyses Neu5Ac from glycoconjugates Homo sapiens GO:0004308 exo-alpha-sialidase activity TAS enables MF false ACCEPT Reactome TAS for NEU1 hydrolysing Neu5Ac from glycoconjugates in the lysosomal multienzyme complex. Correct core molecular function. reaction_mf_exact accepted_mf 1 human NRAS genes/human/NRAS/NRAS-ai-review.yaml NRAS P01111 NCBITaxon:9606 Homo sapiens 36 R-HSA-9649736 RAS intrinsic GTPase activity hydrolyzes GTP to GDP yes reactome/R-HSA-9649736.md RAS intrinsic GTPase activity hydrolyzes GTP to GDP Homo sapiens GO:0003924 GTPase activity TAS enables MF false ACCEPT Reactome attributes GTPase activity to NRAS, its canonical catalytic molecular function. reaction_mf_exact accepted_mf multi_gene_reactome_id 3 human NSDHL genes/human/NSDHL/NSDHL-ai-review.yaml NSDHL Q15738 NCBITaxon:9606 Homo sapiens 16 R-HSA-194642 4-methyl,4-carboxycholesta-8(9),24-dien-3beta-ol is decarboxylated and oxidized to form 4-methylcholesta-8(9),24-dien-3-one yes reactome/R-HSA-194642.md 4-methyl,4-carboxycholesta-8(9),24-dien-3beta-ol is decarboxylated and oxidized to form 4-methylcholesta-8(9),24-dien-3-one Homo sapiens GO:0000252 3-beta-hydroxysteroid dehydrogenase [NAD(P)+]/C4-decarboxylase activity TAS enables MF false ACCEPT Reactome TAS for the C4-decarboxylase activity in the reaction converting 4-methyl,4-carboxycholesta-8(9),24-dien-3beta-ol to 4-methylcholesta-8(9),24-dien-3-one + CO2 + NADH, catalyzed by NSDHL in the ER. reaction_mf_exact accepted_mf local_module_mf 1 cholesterol_synthesis_post_lanosterol:GO:0000252 3-beta-hydroxysteroid dehydrogenase [NAD(P)+]/C4-decarboxylase activity human NSDHL genes/human/NSDHL/NSDHL-ai-review.yaml NSDHL Q15738 NCBITaxon:9606 Homo sapiens 17 R-HSA-194718 4-carboxycholesta-8(9),24-dien-3beta-ol is decarboxylated and oxidized to form cholesta-8(9),24-dien-3-one (zymosterone) yes reactome/R-HSA-194718.md 4-carboxycholesta-8(9),24-dien-3beta-ol is decarboxylated and oxidized to form cholesta-8(9),24-dien-3-one (zymosterone) Homo sapiens GO:0000252 3-beta-hydroxysteroid dehydrogenase [NAD(P)+]/C4-decarboxylase activity TAS enables MF false ACCEPT Reactome TAS for the C4-decarboxylase activity in the reaction converting 4-carboxycholesta-8(9),24-dien-3beta-ol to zymosterone + CO2 + NADH, catalyzed by NSDHL in the ER. reaction_mf_exact accepted_mf 1 human OPLAH genes/human/OPLAH/OPLAH-ai-review.yaml OPLAH O14841 NCBITaxon:9606 Homo sapiens 11 R-HSA-5603208 Defective OPLAH does not hydrolyse OPRO yes reactome/R-HSA-5603208.md Defective OPLAH does not hydrolyse OPRO Homo sapiens GO:0017168 5-oxoprolinase (ATP-hydrolyzing) activity TAS enables MF false ACCEPT "Reactome traceable-author-statement for the 5-oxoprolinase activity (from the reaction ""Defective OPLAH does not hydrolyse OPRO""). Correct core molecular function." reaction_mf_exact accepted_mf reactome_disease_event 1 human OTUD3 genes/human/OTUD3/OTUD3-ai-review.yaml OTUD3 Q5T2D3 NCBITaxon:9606 Homo sapiens 12 R-HSA-6807206 Reactome: Ovarian tumor domain proteases yes reactome/R-HSA-6807206.md USP13 and OTUD3 deubiquitinate PTEN Homo sapiens GO:0004843 cysteine-type deubiquitinase activity TAS enables MF false ACCEPT Reactome annotation of OTUD3 cysteine-type deubiquitinase activity. reaction_mf_exact accepted_mf 1 human OTUD3 genes/human/OTUD3/OTUD3-ai-review.yaml OTUD3 Q5T2D3 NCBITaxon:9606 Homo sapiens 13 R-HSA-8873946 Reactome: Regulation of PTEN stability and activity yes reactome/R-HSA-8873946.md OTUD3 deubiquitinates PTEN Homo sapiens GO:0004843 cysteine-type deubiquitinase activity TAS enables MF false ACCEPT Reactome annotation of OTUD3 cysteine-type deubiquitinase activity (PTEN regulation pathway). reaction_mf_exact accepted_mf 1 human PANK2 genes/human/PANK2/PANK2-ai-review.yaml PANK2 Q9BZ23 NCBITaxon:9606 Homo sapiens 17 R-HSA-196857 PANK2 phosphorylates PanK yes reactome/R-HSA-196857.md PANK2 phosphorylates PanK Homo sapiens GO:0004594 pantothenate kinase activity TAS enables MF false ACCEPT "Reactome-traceable assignment of the core pantothenate kinase activity (""PANK2 phosphorylates PanK""). Consistent with all experimental evidence." reaction_mf_exact accepted_mf 1 human PAPSS1 genes/human/PAPSS1/PAPSS1-ai-review.yaml PAPSS1 O43252 NCBITaxon:9606 Homo sapiens 13 R-HSA-174389 PAPSS1,2 transfer PO4(2-) group from ATP to APS to form PAPS yes reactome/R-HSA-174389.md PAPSS1,2 transfer PO4(2-) group from ATP to APS to form PAPS Homo sapiens GO:0004020 adenylylsulfate kinase activity TAS enables MF false ACCEPT Reactome captures the APS kinase reaction; core MF. Accept. reaction_mf_exact accepted_mf multi_gene_reactome_id 2 human PAPSS1 genes/human/PAPSS1/PAPSS1-ai-review.yaml PAPSS1 O43252 NCBITaxon:9606 Homo sapiens 14 R-HSA-174392 PAPSS1,2 transfer SO4(2-) group to ATP to form APS yes reactome/R-HSA-174392.md PAPSS1,2 transfer SO4(2-) group to ATP to form APS Homo sapiens GO:0004781 sulfate adenylyltransferase (ATP) activity TAS enables MF false ACCEPT Reactome captures the ATP sulfurylase reaction; core MF. Accept. reaction_mf_exact accepted_mf multi_gene_reactome_id 2 human PAPSS2 genes/human/PAPSS2/PAPSS2-ai-review.yaml PAPSS2 O95340 NCBITaxon:9606 Homo sapiens 14 R-HSA-174389 PAPSS1,2 transfer PO4(2-) group from ATP to APS to form PAPS yes reactome/R-HSA-174389.md PAPSS1,2 transfer PO4(2-) group from ATP to APS to form PAPS Homo sapiens GO:0004020 adenylylsulfate kinase activity TAS enables MF false ACCEPT Reactome curation of the APS kinase (adenylyl-sulfate kinase) activity, a core catalytic function of PAPSS2. Retained. reaction_mf_exact accepted_mf multi_gene_reactome_id 2 human PAPSS2 genes/human/PAPSS2/PAPSS2-ai-review.yaml PAPSS2 O95340 NCBITaxon:9606 Homo sapiens 15 R-HSA-3560785 Defective PAPSS2 does not transfer PO4(2-) group from ATP to APS to form PAPS yes reactome/R-HSA-3560785.md Defective PAPSS2 does not transfer PO4(2-) group from ATP to APS to form PAPS Homo sapiens GO:0004020 adenylylsulfate kinase activity TAS enables MF false ACCEPT "Reactome ""Defective PAPSS2"" disease reaction annotating the same core APS kinase activity (the activity lost in SEMD-PA). Molecular function is correct; retained." reaction_mf_exact accepted_mf reactome_disease_event 1 human PAPSS2 genes/human/PAPSS2/PAPSS2-ai-review.yaml PAPSS2 O95340 NCBITaxon:9606 Homo sapiens 16 R-HSA-174392 PAPSS1,2 transfer SO4(2-) group to ATP to form APS yes reactome/R-HSA-174392.md PAPSS1,2 transfer SO4(2-) group to ATP to form APS Homo sapiens GO:0004781 sulfate adenylyltransferase (ATP) activity TAS enables MF false ACCEPT Reactome curation of the ATP sulfurylase (sulfate adenylyltransferase (ATP)) activity, the second core catalytic function of PAPSS2. Retained. reaction_mf_exact accepted_mf multi_gene_reactome_id 2 human PAPSS2 genes/human/PAPSS2/PAPSS2-ai-review.yaml PAPSS2 O95340 NCBITaxon:9606 Homo sapiens 17 R-HSA-3560794 Defective PAPSS2 does not transfer SO4(2-) group to ATP to form APS yes reactome/R-HSA-3560794.md Defective PAPSS2 does not transfer SO4(2-) group to ATP to form APS Homo sapiens GO:0004781 sulfate adenylyltransferase (ATP) activity TAS enables MF false ACCEPT "Reactome ""Defective PAPSS2"" disease reaction annotating the same core ATP sulfurylase activity (the activity lost in SEMD-PA). Molecular function is correct; retained." reaction_mf_exact accepted_mf reactome_disease_event 1 human PEMT genes/human/PEMT/PEMT-ai-review.yaml PEMT Q9UBM1 NCBITaxon:9606 Homo sapiens 13 R-HSA-1483174 PE is methylated to PC by PEMT yes reactome/R-HSA-1483174.md PE is methylated to PC by PEMT Homo sapiens GO:0004608 phosphatidylethanolamine N-methyltransferase activity TAS enables MF false ACCEPT Reactome traceable-author statement for the core molecular function, phosphatidylethanolamine N-methyltransferase activity (PE methylated to PC by PEMT). This is the central catalytic function of the enzyme. Accept as core. reaction_mf_exact accepted_mf 1 human PFAS genes/human/PFAS/PFAS-ai-review.yaml PFAS O15067 NCBITaxon:9606 Homo sapiens 10 R-HSA-73812 FGAR + L-Glutamine + ATP + H2O => FGAM + L-Glutamate + ADP + Pi yes reactome/R-HSA-73812.md FGAR + L-Glutamine + ATP + H2O => FGAM + L-Glutamate + ADP + Pi Homo sapiens GO:0004642 phosphoribosylformylglycinamidine synthase activity TAS enables MF false ACCEPT Reactome traceable assertion of FGAM synthase activity, citing the biochemically characterized human enzyme reaction FGAR + L-glutamine + ATP + H2O -> FGAM + L-glutamate + ADP + Pi. reaction_mf_exact accepted_mf 1 human PGAP1 genes/human/PGAP1/PGAP1-ai-review.yaml PGAP1 Q75T13 NCBITaxon:9606 Homo sapiens 10 R-HSA-162729 uPAR-acyl-GPI + H2O -> uPAR + long-chain fatty acid yes reactome/R-HSA-162729.md uPAR-acyl-GPI + H2O -> uPAR + long-chain fatty acid Homo sapiens GO:0016788 hydrolase activity, acting on ester bonds TAS enables MF false ACCEPT Reactome traceable annotation of ester-bond hydrolase activity, based on the modeled reaction uPAR-acyl-GPI + H2O to uPAR + long-chain fatty acid in the ER. Correct broad classification of the deacylation (ester hydrolysis) reaction. reaction_mf_exact accepted_mf local_module_mf 1 gpi_anchor_remodeling:GO:0160215 deacylase activity human PGM3 genes/human/PGM3/PGM3-ai-review.yaml PGM3 O95394 NCBITaxon:9606 Homo sapiens 10 R-HSA-446185 Isomerization of GlcNAc6P to GlcNAc1P yes reactome/R-HSA-446185.md Isomerization of GlcNAc6P to GlcNAc1P Homo sapiens GO:0004610 phosphoacetylglucosamine mutase activity TAS enables MF false ACCEPT Reactome (TAS) annotation to the core catalytic activity, corresponding to the reaction Isomerization of GlcNAc6P to GlcNAc1P. Correct and consistent with the experimental annotations of the same activity. reaction_mf_exact accepted_mf 1 human PIGB genes/human/PIGB/PIGB-ai-review.yaml PIGB Q92521 NCBITaxon:9606 Homo sapiens 8 R-HSA-162821 mannose (a1-6) (ethanolamineP) mannose (a1-4) glucosaminyl-acyl-PI + dolichol phosphate D-mannose -> mannose (a1-2) mannose (a1-6) (ethanolamineP) mannose (a1-4) glucosaminyl-acyl-PI + dolichol phosphate yes reactome/R-HSA-162821.md mannose (a1-6) (ethanolamineP) mannose (a1-4) glucosaminyl-acyl-PI + dolichol phosphate D-mannose -> mannose (a1-2) mannose (a1-6) (ethanolamineP) mannose (a1-4) glucosaminyl-acyl-PI + dolichol phosphate Homo sapiens GO:0004376 GPI mannosyltransferase activity TAS enables MF false ACCEPT Reactome-traceable assertion (the reaction adding the third mannose of the GPI anchor) that PIGB has GPI mannosyltransferase activity; a correct family-level molecular-function term for this enzyme. reaction_mf_exact accepted_mf local_module_mf 1 gpi_anchor_core_glycan_assembly:GO:0120564 dol-P-Man:Man(2)GlcN-acyl-PI alpha-1,2-mannosyltransferase activity human PIGK genes/human/PIGK/PIGK-ai-review.yaml PIGK Q92643 NCBITaxon:9606 Homo sapiens 39 R-HSA-162836 uPAR precursor + acyl-GPI -> uPAR-acyl-GPI + uPAR propeptide yes reactome/R-HSA-162836.md uPAR precursor + acyl-GPI -> uPAR-acyl-GPI + uPAR propeptide Homo sapiens GO:0003923 GPI-anchor transamidase activity TAS enables MF false ACCEPT Reactome traceable annotation for the GPI-T-catalyzed reaction (uPAR precursor + acyl-GPI -> uPAR-acyl-GPI + propeptide), mapped to GPI-anchor transamidase activity - the exact GOA core MF term. reaction_mf_exact accepted_mf 1 human PIGL genes/human/PIGL/PIGL-ai-review.yaml PIGL Q9Y2B2 NCBITaxon:9606 Homo sapiens 7 R-HSA-162857 N-acetylglucosaminyl-PI + H2O -> glucosaminyl-PI + acetate yes reactome/R-HSA-162857.md N-acetylglucosaminyl-PI + H2O -> glucosaminyl-PI + acetate Homo sapiens GO:0000225 N-acetylglucosaminylphosphatidylinositol deacetylase activity TAS enables MF false ACCEPT Reactome traceable annotation of the second-step deacetylase reaction (N-acetylglucosaminyl-PI + H2O -> glucosaminyl-PI + acetate) catalyzed by PIG-L. Correct core molecular function. reaction_mf_exact accepted_mf local_module_mf 1 gpi_anchor_core_glycan_assembly:GO:0000225 N-acetylglucosaminylphosphatidylinositol deacetylase activity human PIGN genes/human/PIGN/PIGN-ai-review.yaml PIGN O95427 NCBITaxon:9606 Homo sapiens 15 R-HSA-162798 mannose(a1-4)glucosaminyl-acyl-PI + phosphatidylethanolamine -> (ethanolamineP) mannose(al1-4)glucosaminyl-acyl-PI + diacylglycerol yes reactome/R-HSA-162798.md mannose(a1-4)glucosaminyl-acyl-PI + phosphatidylethanolamine -> (ethanolamineP) mannose(al1-4)glucosaminyl-acyl-PI + diacylglycerol Homo sapiens GO:0051377 mannose-ethanolamine phosphotransferase activity TAS enables MF false ACCEPT Reactome traceable-author annotation of the specific EtNP-transfer reaction catalyzed by PIGN (phosphoethanolamine from PE onto the first mannose of the GPI precursor). Directly supports the core molecular function. reaction_mf_exact accepted_mf local_module_mf 1 gpi_anchor_ethanolamine_phosphate:GO:0051377 mannose-ethanolamine phosphotransferase activity human PIGV genes/human/PIGV/PIGV-ai-review.yaml PIGV Q9NUD9 NCBITaxon:9606 Homo sapiens 12 R-HSA-162873 (ethanolamineP) mannose (a1-4) glucosaminyl-acyl-PI + dolichol phosphate D-mannose -> mannose (a1-6) (ethanolamineP) mannose (a1-4) glucosaminyl-acyl-PI + dolichol phosphate yes reactome/R-HSA-162873.md (ethanolamineP) mannose (a1-4) glucosaminyl-acyl-PI + dolichol phosphate D-mannose -> mannose (a1-6) (ethanolamineP) mannose (a1-4) glucosaminyl-acyl-PI + dolichol phosphate Homo sapiens GO:0004376 GPI mannosyltransferase activity TAS enables MF false ACCEPT Reactome (TAS) assignment of GPI mannosyltransferase activity to PIGV for the second-mannose-addition reaction. Correct parent of the specific GPI-MT-II activity (GO:0120563). reaction_mf_exact accepted_mf local_module_mf 1 gpi_anchor_core_glycan_assembly:GO:0120563 dol-P-Man:Man(1)GlcN-acyl-PI alpha-1,6-mannosyltransferase activity human PIK3C3 genes/human/PIK3C3/PIK3C3-ai-review.yaml PIK3C3 Q8NEB9 NCBITaxon:9606 Homo sapiens 70 R-HSA-5672012 Beclin-1 complex phosphorylates PtdIns yes reactome/R-HSA-5672012.md Beclin-1 complex phosphorylates PtdIns Homo sapiens GO:0016303 1-phosphatidylinositol-3-kinase activity TAS enables MF false ACCEPT The Reactome TAS row correctly captures the core VPS34 lipid kinase activity. reaction_mf_exact accepted_mf 1 human PIK3C3 genes/human/PIK3C3/PIK3C3-ai-review.yaml PIK3C3 Q8NEB9 NCBITaxon:9606 Homo sapiens 71 R-HSA-6798174 PIK3C3:PIK3R4 phosphorylates PI to PI3P yes reactome/R-HSA-6798174.md PIK3C3:PIK3R4 phosphorylates PI to PI3P Homo sapiens GO:0016303 1-phosphatidylinositol-3-kinase activity TAS enables MF false ACCEPT The Reactome TAS row correctly captures the core VPS34 lipid kinase activity. reaction_mf_exact accepted_mf 1 human PIK3CA genes/human/PIK3CA/PIK3CA-ai-review.yaml PIK3CA P42336 NCBITaxon:9606 Homo sapiens 219 R-HSA-177939 PI3K converts phosphatidylinositol-4,5-bisphosphate (PIP2) to phosphatidylinositol-3,4,5-trisphosphate (PIP3) yes reactome/R-HSA-177939.md PI3K converts phosphatidylinositol-4,5-bisphosphate (PIP2) to phosphatidylinositol-3,4,5-trisphosphate (PIP3) Homo sapiens GO:0046934 1-phosphatidylinositol-4,5-bisphosphate 3-kinase activity TAS enables MF false ACCEPT As the catalytic p110alpha subunit of class IA PI3K, PIK3CA phosphorylates the 3-position of PI(4,5)P2 using ATP to generate the second messenger PIP3; this is its primary molecular function. reaction_mf_exact accepted_mf 1 human PIK3CA genes/human/PIK3CA/PIK3CA-ai-review.yaml PIK3CA P42336 NCBITaxon:9606 Homo sapiens 220 R-HSA-1839107 BCR-FGFR1-associated PI3K phosphorylates PIP2 to PIP3 yes reactome/R-HSA-1839107.md BCR-FGFR1-associated PI3K phosphorylates PIP2 to PIP3 Homo sapiens GO:0046934 1-phosphatidylinositol-4,5-bisphosphate 3-kinase activity TAS enables MF false ACCEPT As the catalytic p110alpha subunit of class IA PI3K, PIK3CA phosphorylates the 3-position of PI(4,5)P2 using ATP to generate the second messenger PIP3; this is its primary molecular function. reaction_mf_exact accepted_mf 1 human PIK3CA genes/human/PIK3CA/PIK3CA-ai-review.yaml PIK3CA P42336 NCBITaxon:9606 Homo sapiens 221 R-HSA-186800 PI3K catalyses the phosphorylation of PIP2 to PIP3 yes reactome/R-HSA-186800.md PI3K catalyses the phosphorylation of PIP2 to PIP3 Homo sapiens GO:0046934 1-phosphatidylinositol-4,5-bisphosphate 3-kinase activity TAS enables MF false ACCEPT As the catalytic p110alpha subunit of class IA PI3K, PIK3CA phosphorylates the 3-position of PI(4,5)P2 using ATP to generate the second messenger PIP3; this is its primary molecular function. reaction_mf_exact accepted_mf 1 human PIK3CA genes/human/PIK3CA/PIK3CA-ai-review.yaml PIK3CA P42336 NCBITaxon:9606 Homo sapiens 222 R-HSA-201510 PI3K synthesizes PIP3 downstream of ALK yes reactome/R-HSA-201510.md PI3K synthesizes PIP3 downstream of ALK Homo sapiens GO:0046934 1-phosphatidylinositol-4,5-bisphosphate 3-kinase activity TAS enables MF false ACCEPT As the catalytic p110alpha subunit of class IA PI3K, PIK3CA phosphorylates the 3-position of PI(4,5)P2 using ATP to generate the second messenger PIP3; this is its primary molecular function. reaction_mf_exact accepted_mf 1 human PIK3CA genes/human/PIK3CA/PIK3CA-ai-review.yaml PIK3CA P42336 NCBITaxon:9606 Homo sapiens 223 R-HSA-202365 PI3K bound to TRAT1 phosphorylates PIP2 to PIP3 yes reactome/R-HSA-202365.md PI3K bound to TRAT1 phosphorylates PIP2 to PIP3 Homo sapiens GO:0046934 1-phosphatidylinositol-4,5-bisphosphate 3-kinase activity TAS enables MF false ACCEPT As the catalytic p110alpha subunit of class IA PI3K, PIK3CA phosphorylates the 3-position of PI(4,5)P2 using ATP to generate the second messenger PIP3; this is its primary molecular function. reaction_mf_exact accepted_mf 1 human PIK3CA genes/human/PIK3CA/PIK3CA-ai-review.yaml PIK3CA P42336 NCBITaxon:9606 Homo sapiens 224 R-HSA-5655235 Activated FGFR4 mutant-associated PI3K phosphorylates PIP2 to PIP3 yes reactome/R-HSA-5655235.md Activated FGFR4 mutant-associated PI3K phosphorylates PIP2 to PIP3 Homo sapiens GO:0046934 1-phosphatidylinositol-4,5-bisphosphate 3-kinase activity TAS enables MF false ACCEPT As the catalytic p110alpha subunit of class IA PI3K, PIK3CA phosphorylates the 3-position of PI(4,5)P2 using ATP to generate the second messenger PIP3; this is its primary molecular function. reaction_mf_exact accepted_mf 1 human PIK3CA genes/human/PIK3CA/PIK3CA-ai-review.yaml PIK3CA P42336 NCBITaxon:9606 Homo sapiens 225 R-HSA-5655289 Activated FGFR3 point, translocation and fusion mutant-associated PI3K phosphorylates PIP2 to PIP3 yes reactome/R-HSA-5655289.md Activated FGFR3 point, translocation and fusion mutant-associated PI3K phosphorylates PIP2 to PIP3 Homo sapiens GO:0046934 1-phosphatidylinositol-4,5-bisphosphate 3-kinase activity TAS enables MF false ACCEPT As the catalytic p110alpha subunit of class IA PI3K, PIK3CA phosphorylates the 3-position of PI(4,5)P2 using ATP to generate the second messenger PIP3; this is its primary molecular function. reaction_mf_exact accepted_mf 1 human PIK3CA genes/human/PIK3CA/PIK3CA-ai-review.yaml PIK3CA P42336 NCBITaxon:9606 Homo sapiens 226 R-HSA-5655290 Activated FGFR1 mutant-associated PI3K phosphorylates PIP2 to PIP3 yes reactome/R-HSA-5655290.md Activated FGFR1 mutant-associated PI3K phosphorylates PIP2 to PIP3 Homo sapiens GO:0046934 1-phosphatidylinositol-4,5-bisphosphate 3-kinase activity TAS enables MF false ACCEPT As the catalytic p110alpha subunit of class IA PI3K, PIK3CA phosphorylates the 3-position of PI(4,5)P2 using ATP to generate the second messenger PIP3; this is its primary molecular function. reaction_mf_exact accepted_mf 1 human PIK3CA genes/human/PIK3CA/PIK3CA-ai-review.yaml PIK3CA P42336 NCBITaxon:9606 Homo sapiens 227 R-HSA-5655323 Activated FGFR2 mutant-associated PI3K phosphorylates PIP2 to PIP3 yes reactome/R-HSA-5655323.md Activated FGFR2 mutant-associated PI3K phosphorylates PIP2 to PIP3 Homo sapiens GO:0046934 1-phosphatidylinositol-4,5-bisphosphate 3-kinase activity TAS enables MF false ACCEPT As the catalytic p110alpha subunit of class IA PI3K, PIK3CA phosphorylates the 3-position of PI(4,5)P2 using ATP to generate the second messenger PIP3; this is its primary molecular function. reaction_mf_exact accepted_mf 1 human PIK3CD genes/human/PIK3CD/PIK3CD-ai-review.yaml PIK3CD O00329 NCBITaxon:9606 Homo sapiens 51 R-HSA-389158 CD28 bound PI3K phosphorylates PIP2 to PIP3 yes reactome/R-HSA-389158.md CD28 bound PI3K phosphorylates PIP2 to PIP3 Homo sapiens GO:0046934 1-phosphatidylinositol-4,5-bisphosphate 3-kinase activity TAS enables MF false ACCEPT Core specific molecular function: p110delta phosphorylates PI(4,5)P2 to generate PIP3. reaction_mf_exact accepted_mf 1 human PIP5K1B genes/human/PIP5K1B/PIP5K1B-ai-review.yaml PIP5K1B O14986 NCBITaxon:9606 Homo sapiens 12 R-HSA-1676082 PI4P is phosphorylated to PI(4,5)P2 by PIP5K1A-C at the plasma membrane yes reactome/R-HSA-1676082.md PI4P is phosphorylated to PI(4,5)P2 by PIP5K1A-C at the plasma membrane Homo sapiens GO:0016308 1-phosphatidylinositol-4-phosphate 5-kinase activity TAS enables MF false ACCEPT This is the core PIP5K1B molecular function: phosphorylation of PI4P at the 5 position to generate PI(4,5)P2/PIP2. reaction_mf_exact accepted_mf 1 human PKMYT1 genes/human/PKMYT1/PKMYT1-ai-review.yaml PKMYT1 Q99640 NCBITaxon:9606 Homo sapiens 11 R-HSA-170055 Myt-1 mediated phosphorylation of Cyclin B:Cdc2 complexes yes reactome/R-HSA-170055.md Myt-1 mediated phosphorylation of Cyclin B:Cdc2 complexes Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Reactome reaction 'Myt-1 mediated phosphorylation of Cyclin B:Cdc2 complexes' - the canonical PKMYT1 reaction. reaction_mf_exact accepted_mf 1 human PKMYT1 genes/human/PKMYT1/PKMYT1-ai-review.yaml PKMYT1 Q99640 NCBITaxon:9606 Homo sapiens 12 R-HSA-170116 PKMYT1 phosphorylates CCNA:CDK1 yes reactome/R-HSA-170116.md PKMYT1 phosphorylates CCNA:CDK1 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Reactome reaction 'PKMYT1 phosphorylates CCNA:CDK1' - inhibitory phosphorylation of cyclin A-bound CDK1, consistent with PKMYT1's requirement for cyclin-bound CDK1 rather than for cyclin B specifically. reaction_mf_exact accepted_mf 1 human PLCG2 genes/human/PLCG2/PLCG2-ai-review.yaml PLCG2 P16885 NCBITaxon:9606 Homo sapiens 56 R-HSA-2730847 Hydrolysis of PIP2 by PLCG yes reactome/R-HSA-2730847.md Hydrolysis of PIP2 by PLCG Homo sapiens GO:0004435 phosphatidylinositol-4,5-bisphosphate phospholipase C activity TAS enables MF false ACCEPT PLCG2 is a phosphoinositide-specific phospholipase C that hydrolyzes PIP2 to IP3 and DAG after receptor-induced activation. reaction_mf_exact accepted_mf 1 human PLCG2 genes/human/PLCG2/PLCG2-ai-review.yaml PLCG2 P16885 NCBITaxon:9606 Homo sapiens 57 R-HSA-5607735 p-Y753,Y759-PLCG2 hydrolyses PIP2 yes reactome/R-HSA-5607735.md p-Y753,Y759-PLCG2 hydrolyses PIP2 Homo sapiens GO:0004435 phosphatidylinositol-4,5-bisphosphate phospholipase C activity TAS enables MF false ACCEPT PLCG2 is a phosphoinositide-specific phospholipase C that hydrolyzes PIP2 to IP3 and DAG after receptor-induced activation. reaction_mf_exact accepted_mf 1 human PLK1 genes/human/PLK1/PLK1-ai-review.yaml PLK1 P53350 NCBITaxon:9606 Homo sapiens 61 R-HSA-156673 Regulation of KIF23 (MKLP1) by phosphorylation yes reactome/R-HSA-156673.md Regulation of KIF23 (MKLP1) by phosphorylation Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Reactome reaction 'Regulation of KIF23 (MKLP1) by phosphorylation'. Protein serine/threonine kinase activity is the defining molecular function of PLK1; the kinase domain (residues ~39-325) phosphorylates Ser/Thr on substrates docked via the C-terminal polo-box domain. reaction_mf_exact accepted_mf 1 human PLK1 genes/human/PLK1/PLK1-ai-review.yaml PLK1 P53350 NCBITaxon:9606 Homo sapiens 62 R-HSA-156678 Activation of Cdc25C yes reactome/R-HSA-156678.md Activation of Cdc25C Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Reactome 'Activation of Cdc25C': PLK1 phosphorylates and activates CDC25C. Protein serine/threonine kinase activity is the defining molecular function of PLK1; the kinase domain (residues ~39-325) phosphorylates Ser/Thr on substrates docked via the C-terminal polo-box domain. reaction_mf_exact accepted_mf 1 human PLK1 genes/human/PLK1/PLK1-ai-review.yaml PLK1 P53350 NCBITaxon:9606 Homo sapiens 63 R-HSA-156682 PLK1 phosphorylates NUDC yes reactome/R-HSA-156682.md PLK1 phosphorylates NUDC Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Reactome reaction 'PLK1 phosphorylates NUDC'. Protein serine/threonine kinase activity is the defining molecular function of PLK1; the kinase domain (residues ~39-325) phosphorylates Ser/Thr on substrates docked via the C-terminal polo-box domain. reaction_mf_exact accepted_mf 1 human PLK1 genes/human/PLK1/PLK1-ai-review.yaml PLK1 P53350 NCBITaxon:9606 Homo sapiens 64 R-HSA-156699 Inactivation of Wee1 kinase yes reactome/R-HSA-156699.md Inactivation of Wee1 kinase Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Reactome reaction 'Inactivation of Wee1 kinase'. Protein serine/threonine kinase activity is the defining molecular function of PLK1; the kinase domain (residues ~39-325) phosphorylates Ser/Thr on substrates docked via the C-terminal polo-box domain. reaction_mf_exact accepted_mf 1 human PLK1 genes/human/PLK1/PLK1-ai-review.yaml PLK1 P53350 NCBITaxon:9606 Homo sapiens 65 R-HSA-156723 Regulation of KIF20A (MKL2) by phosphorylation yes reactome/R-HSA-156723.md Regulation of KIF20A (MKL2) by phosphorylation Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Reactome reaction 'Regulation of KIF20A (MKL2) by phosphorylation'. Protein serine/threonine kinase activity is the defining molecular function of PLK1; the kinase domain (residues ~39-325) phosphorylates Ser/Thr on substrates docked via the C-terminal polo-box domain. reaction_mf_exact accepted_mf 1 human PLK1 genes/human/PLK1/PLK1-ai-review.yaml PLK1 P53350 NCBITaxon:9606 Homo sapiens 66 R-HSA-162657 Inactivation of Myt1 kinase yes reactome/R-HSA-162657.md Inactivation of Myt1 kinase Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Reactome reaction 'Inactivation of Myt1 kinase'. Protein serine/threonine kinase activity is the defining molecular function of PLK1; the kinase domain (residues ~39-325) phosphorylates Ser/Thr on substrates docked via the C-terminal polo-box domain. reaction_mf_exact accepted_mf 1 human PLK1 genes/human/PLK1/PLK1-ai-review.yaml PLK1 P53350 NCBITaxon:9606 Homo sapiens 67 R-HSA-163010 Down Regulation of Emi1 through Phosphorylation of Emi1 yes reactome/R-HSA-163010.md Down Regulation of Emi1 through Phosphorylation of Emi1 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Reactome reaction 'Down Regulation of Emi1 through Phosphorylation of Emi1'. Protein serine/threonine kinase activity is the defining molecular function of PLK1; the kinase domain (residues ~39-325) phosphorylates Ser/Thr on substrates docked via the C-terminal polo-box domain. reaction_mf_exact accepted_mf 1 human PLK1 genes/human/PLK1/PLK1-ai-review.yaml PLK1 P53350 NCBITaxon:9606 Homo sapiens 68 R-HSA-1638803 Phosphorylation of cohesin by PLK1 at centromeres yes reactome/R-HSA-1638803.md Phosphorylation of cohesin by PLK1 at centromeres Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Reactome reaction 'Phosphorylation of cohesin by PLK1 at centromeres'. Protein serine/threonine kinase activity is the defining molecular function of PLK1; the kinase domain (residues ~39-325) phosphorylates Ser/Thr on substrates docked via the C-terminal polo-box domain. reaction_mf_exact accepted_mf 1 human PLK1 genes/human/PLK1/PLK1-ai-review.yaml PLK1 P53350 NCBITaxon:9606 Homo sapiens 69 R-HSA-174119 Free APC/C phosphorylated by Plk1 yes reactome/R-HSA-174119.md Free APC/C phosphorylated by Plk1 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Reactome reaction 'Free APC/C phosphorylated by Plk1'. Protein serine/threonine kinase activity is the defining molecular function of PLK1; the kinase domain (residues ~39-325) phosphorylates Ser/Thr on substrates docked via the C-terminal polo-box domain. reaction_mf_exact accepted_mf 1 human PLK1 genes/human/PLK1/PLK1-ai-review.yaml PLK1 P53350 NCBITaxon:9606 Homo sapiens 70 R-HSA-174174 Phosphorylation of the Emi1 DSGxxS degron by Plk1 yes reactome/R-HSA-174174.md Phosphorylation of the Emi1 DSGxxS degron by Plk1 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Reactome reaction 'Phosphorylation of the Emi1 DSGxxS degron by Plk1'. Protein serine/threonine kinase activity is the defining molecular function of PLK1; the kinase domain (residues ~39-325) phosphorylates Ser/Thr on substrates docked via the C-terminal polo-box domain. reaction_mf_exact accepted_mf 1 human PLK1 genes/human/PLK1/PLK1-ai-review.yaml PLK1 P53350 NCBITaxon:9606 Homo sapiens 71 R-HSA-2214351 PLK1 phosphorylates GORASP1 yes reactome/R-HSA-2214351.md PLK1 phosphorylates GORASP1 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Reactome reaction 'PLK1 phosphorylates GORASP1'. Protein serine/threonine kinase activity is the defining molecular function of PLK1; the kinase domain (residues ~39-325) phosphorylates Ser/Thr on substrates docked via the C-terminal polo-box domain. reaction_mf_exact accepted_mf 1 human PLK1 genes/human/PLK1/PLK1-ai-review.yaml PLK1 P53350 NCBITaxon:9606 Homo sapiens 72 R-HSA-2294580 PLK1 hyperphosphorylates Condensin II complex yes reactome/R-HSA-2294580.md PLK1 hyperphosphorylates Condensin II complex Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Reactome reaction 'PLK1 hyperphosphorylates Condensin II complex'. Protein serine/threonine kinase activity is the defining molecular function of PLK1; the kinase domain (residues ~39-325) phosphorylates Ser/Thr on substrates docked via the C-terminal polo-box domain. reaction_mf_exact accepted_mf 1 human PLK1 genes/human/PLK1/PLK1-ai-review.yaml PLK1 P53350 NCBITaxon:9606 Homo sapiens 73 R-HSA-2466068 Phosphorylation of cohesin by PLK1 at chromosomal arms yes reactome/R-HSA-2466068.md Phosphorylation of cohesin by PLK1 at chromosomal arms Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Reactome reaction 'Phosphorylation of cohesin by PLK1 at chromosomal arms'. Protein serine/threonine kinase activity is the defining molecular function of PLK1; the kinase domain (residues ~39-325) phosphorylates Ser/Thr on substrates docked via the C-terminal polo-box domain. reaction_mf_exact accepted_mf 1 human PLK1 genes/human/PLK1/PLK1-ai-review.yaml PLK1 P53350 NCBITaxon:9606 Homo sapiens 74 R-HSA-2562526 PLK1 phosphorylates OPTN yes reactome/R-HSA-2562526.md PLK1 phosphorylates OPTN Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Reactome reaction 'PLK1 phosphorylates OPTN'. Protein serine/threonine kinase activity is the defining molecular function of PLK1; the kinase domain (residues ~39-325) phosphorylates Ser/Thr on substrates docked via the C-terminal polo-box domain. reaction_mf_exact accepted_mf 1 human PLK1 genes/human/PLK1/PLK1-ai-review.yaml PLK1 P53350 NCBITaxon:9606 Homo sapiens 75 R-HSA-2984226 PLK1 phosphorylates NEK9 yes reactome/R-HSA-2984226.md PLK1 phosphorylates NEK9 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Reactome reaction 'PLK1 phosphorylates NEK9'. Protein serine/threonine kinase activity is the defining molecular function of PLK1; the kinase domain (residues ~39-325) phosphorylates Ser/Thr on substrates docked via the C-terminal polo-box domain. reaction_mf_exact accepted_mf 1 human PLK1 genes/human/PLK1/PLK1-ai-review.yaml PLK1 P53350 NCBITaxon:9606 Homo sapiens 76 R-HSA-380272 Plk1-mediated phosphorylation of Nlp yes reactome/R-HSA-380272.md Plk1-mediated phosphorylation of Nlp Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Reactome 'Plk1-mediated phosphorylation of Nlp': PLK1 phosphorylates ninein-like protein (NINL), displacing it from maturing centrosomes. Protein serine/threonine kinase activity is the defining molecular function of PLK1; the kinase domain (residues ~39-325) phosphorylates Ser/Thr on substrates docked via the C-terminal polo-box domain. reaction_mf_exact accepted_mf 1 human PLK1 genes/human/PLK1/PLK1-ai-review.yaml PLK1 P53350 NCBITaxon:9606 Homo sapiens 77 R-HSA-9851972 PLK1 phosphorylates FIRRM at S43 yes reactome/R-HSA-9851972.md PLK1 phosphorylates FIRRM at S43 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Reactome reaction 'PLK1 phosphorylates FIRRM at S43'. Protein serine/threonine kinase activity is the defining molecular function of PLK1; the kinase domain (residues ~39-325) phosphorylates Ser/Thr on substrates docked via the C-terminal polo-box domain. reaction_mf_exact accepted_mf 1 human PLK1 genes/human/PLK1/PLK1-ai-review.yaml PLK1 P53350 NCBITaxon:9606 Homo sapiens 78 R-HSA-9853369 PLK1 phosphorylates FIRMM at S744 yes reactome/R-HSA-9853369.md PLK1 phosphorylates FIRMM at S744 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Reactome reaction 'PLK1 phosphorylates FIRMM at S744'. Protein serine/threonine kinase activity is the defining molecular function of PLK1; the kinase domain (residues ~39-325) phosphorylates Ser/Thr on substrates docked via the C-terminal polo-box domain. reaction_mf_exact accepted_mf 1 human PMM2 genes/human/PMM2/PMM2-ai-review.yaml PMM2 O15305 NCBITaxon:9606 Homo sapiens 16 R-HSA-3781926 Defective PMM2 does not isomerise Man6P to Man1P yes reactome/R-HSA-3781926.md Defective PMM2 does not isomerise Man6P to Man1P Homo sapiens GO:0004615 phosphomannomutase activity TAS enables MF false ACCEPT Reactome-curated phosphomannomutase activity (Man6P->Man1P) for PMM2. Concordant with the experimental and IBA/IEA annotations of the same term. reaction_mf_exact accepted_mf reactome_disease_event 1 human PMPCB genes/human/PMPCB/PMPCB-ai-review.yaml PMPCB O75439 NCBITaxon:9606 Homo sapiens 14 R-HSA-8949649 PMPCA:PMPCB cleaves the transit peptide of proSMDT1 (proEMRE) yes reactome/R-HSA-8949649.md PMPCA:PMPCB cleaves the transit peptide of proSMDT1 (proEMRE) Homo sapiens GO:0004222 metalloendopeptidase activity TAS enables MF false ACCEPT Correct and core. PMPCB is the catalytic Zn2+-dependent metalloendopeptidase subunit of MPP. reaction_mf_exact accepted_mf 1 human PMVK genes/human/PMVK/PMVK-ai-review.yaml PMVK Q15126 NCBITaxon:9606 Homo sapiens 11 R-HSA-191422 Mevalonate-5-phosphate is further phosphorylated yes reactome/R-HSA-191422.md Mevalonate-5-phosphate is further phosphorylated Homo sapiens GO:0004631 phosphomevalonate kinase activity TAS enables MF false ACCEPT "Reactome reaction ""Mevalonate-5-phosphate is further phosphorylated"" attributes the phosphomevalonate kinase activity to PMVK. Correct core molecular function." reaction_mf_exact accepted_mf local_module_mf 1 mevalonate_pathway:GO:0004631 phosphomevalonate kinase activity human PNMT genes/human/PNMT/PNMT-ai-review.yaml PNMT P11086 NCBITaxon:9606 Homo sapiens 8 R-HSA-209903 Noradrenaline is converted to adrenaline yes reactome/R-HSA-209903.md Noradrenaline is converted to adrenaline Homo sapiens GO:0004603 phenylethanolamine N-methyltransferase activity TAS enables MF false ACCEPT "Reactome curation of the reaction ""Noradrenaline is converted to adrenaline"" attributes PNMT's transmethylase activity. Correct core molecular function." reaction_mf_exact accepted_mf 1 human PNPLA3 genes/human/PNPLA3/PNPLA3-ai-review.yaml PNPLA3 Q9NST1 NCBITaxon:9606 Homo sapiens 27 R-HSA-1482777 TAG is hydrolyzed to DAG by PNPLA2/3 yes reactome/R-HSA-1482777.md TAG is hydrolyzed to DAG by PNPLA2/3 Homo sapiens GO:0004806 triacylglycerol lipase activity TAS enables MF false ACCEPT Triacylglycerol lipase activity, the activity PNPLA3 shares with its closest paralogue PNPLA2/ATGL. reaction_mf_exact accepted_mf 1 human POMT1 genes/human/POMT1/POMT1-ai-review.yaml POMT1 Q9Y6A1 NCBITaxon:9606 Homo sapiens 16 R-HSA-9816277 CDH1 is O-manosylated yes reactome/R-HSA-9816277.md CDH1 is O-manosylated Homo sapiens GO:0004169 dolichyl-phosphate-mannose-protein mannosyltransferase activity TAS enables MF false ACCEPT O-mannosyltransferase activity - core enzymatic function. reaction_mf_exact accepted_mf 1 human PPP2CA genes/human/PPP2CA/PPP2CA-ai-review.yaml PPP2CA P67775 NCBITaxon:9606 Homo sapiens 81 R-HSA-9865226 PP2A dephosphorylates serine-127 of YAP1 yes reactome/R-HSA-9865226.md PP2A dephosphorylates serine-127 of YAP1 Homo sapiens GO:0004722 protein serine/threonine phosphatase activity TAS enables MF false ACCEPT PPP2CA is the catalytic subunit of PP2A, a well-established serine/threonine phosphatase (EC 3.1.3.16). This is the core molecular function, supported by extensive biochemical, structural, and genetic evidence. reaction_mf_exact accepted_mf 1 human PTEN genes/human/PTEN/PTEN-ai-review.yaml PTEN P60484 NCBITaxon:9606 Homo sapiens 57 R-HSA-199456 PTEN dephosphorylates PIP3 yes reactome/R-HSA-199456.md PTEN dephosphorylates PIP3 Homo sapiens GO:0016314 phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity TAS enables MF false ACCEPT PTEN's primary enzymatic activity. Duplicate of IBA and IDA annotations. reaction_mf_exact accepted_mf 1 human PTEN genes/human/PTEN/PTEN-ai-review.yaml PTEN P60484 NCBITaxon:9606 Homo sapiens 67 R-HSA-1676149 PI(3,4)P2 is dephosphorylated to PI4P by PTEN at the plasma membrane yes reactome/R-HSA-1676149.md PI(3,4)P2 is dephosphorylated to PI4P by PTEN at the plasma membrane Homo sapiens GO:0051800 phosphatidylinositol-3,4-bisphosphate 3-phosphatase activity TAS enables MF false ACCEPT PI(3,4)P2 3-phosphatase activity is documented for PTEN. Duplicate of IEA annotation. reaction_mf_exact accepted_mf 1 human PTPN11 genes/human/PTPN11/PTPN11-ai-review.yaml PTPN11 Q06124 NCBITaxon:9606 Homo sapiens 95 R-HSA-1549564 PPTN11 dephosphorylates SPRY2 yes reactome/R-HSA-1549564.md PPTN11 dephosphorylates SPRY2 Homo sapiens GO:0004725 protein tyrosine phosphatase activity TAS enables MF false ACCEPT Protein tyrosine phosphatase activity is the established catalytic activity of SHP2. reaction_mf_exact accepted_mf 1 human PTPN11 genes/human/PTPN11/PTPN11-ai-review.yaml PTPN11 Q06124 NCBITaxon:9606 Homo sapiens 96 R-HSA-389758 Dephosphorylation of CD3-zeta by PD-1 bound phosphatases yes reactome/R-HSA-389758.md Dephosphorylation of CD3-zeta by PD-1 bound phosphatases Homo sapiens GO:0004725 protein tyrosine phosphatase activity TAS enables MF false ACCEPT Protein tyrosine phosphatase activity is the established catalytic activity of SHP2. reaction_mf_exact accepted_mf multi_gene_reactome_id 2 human PTPN11 genes/human/PTPN11/PTPN11-ai-review.yaml PTPN11 Q06124 NCBITaxon:9606 Homo sapiens 97 R-HSA-914036 SHP1 and SHP2 dephosphorylate Y628 of IL3RB yes reactome/R-HSA-914036.md SHP1 and SHP2 dephosphorylate Y628 of IL3RB Homo sapiens GO:0004725 protein tyrosine phosphatase activity TAS enables MF false ACCEPT Protein tyrosine phosphatase activity is the established catalytic activity of SHP2. reaction_mf_exact accepted_mf multi_gene_reactome_id 2 human PTPN11 genes/human/PTPN11/PTPN11-ai-review.yaml PTPN11 Q06124 NCBITaxon:9606 Homo sapiens 98 R-HSA-997309 Dephosphorylation of STAT1 by SHP2 yes reactome/R-HSA-997309.md Dephosphorylation of STAT1 by SHP2 Homo sapiens GO:0004725 protein tyrosine phosphatase activity TAS enables MF false ACCEPT Protein tyrosine phosphatase activity is the established catalytic activity of SHP2. reaction_mf_exact accepted_mf 1 human PTPN11 genes/human/PTPN11/PTPN11-ai-review.yaml PTPN11 Q06124 NCBITaxon:9606 Homo sapiens 114 R-HSA-177923 Sustained activation of SRC kinase by SHP2 yes reactome/R-HSA-177923.md Sustained activation of SRC kinase by SHP2 Homo sapiens GO:0004726 non-membrane spanning protein tyrosine phosphatase activity TAS enables MF false ACCEPT Non-membrane-spanning protein tyrosine phosphatase activity is SHP2's defining catalytic function. reaction_mf_exact accepted_mf 1 human PTPN11 genes/human/PTPN11/PTPN11-ai-review.yaml PTPN11 Q06124 NCBITaxon:9606 Homo sapiens 115 R-HSA-177924 Dephosphorylation of Gab1 by SHP2 yes reactome/R-HSA-177924.md Dephosphorylation of Gab1 by SHP2 Homo sapiens GO:0004726 non-membrane spanning protein tyrosine phosphatase activity TAS enables MF false ACCEPT Non-membrane-spanning protein tyrosine phosphatase activity is SHP2's defining catalytic function. reaction_mf_exact accepted_mf 1 human PTPN11 genes/human/PTPN11/PTPN11-ai-review.yaml PTPN11 Q06124 NCBITaxon:9606 Homo sapiens 116 R-HSA-177926 Dephosphorylation of PAG by SHP2 yes reactome/R-HSA-177926.md Dephosphorylation of PAG by SHP2 Homo sapiens GO:0004726 non-membrane spanning protein tyrosine phosphatase activity TAS enables MF false ACCEPT Non-membrane-spanning protein tyrosine phosphatase activity is SHP2's defining catalytic function. reaction_mf_exact accepted_mf 1 human PTPN11 genes/human/PTPN11/PTPN11-ai-review.yaml PTPN11 Q06124 NCBITaxon:9606 Homo sapiens 117 R-HSA-177935 SHP2 dephosphorylates Tyr 992 on EGFR yes reactome/R-HSA-177935.md SHP2 dephosphorylates Tyr 992 on EGFR Homo sapiens GO:0004726 non-membrane spanning protein tyrosine phosphatase activity TAS enables MF false ACCEPT Non-membrane-spanning protein tyrosine phosphatase activity is SHP2's defining catalytic function. reaction_mf_exact accepted_mf 1 human PTPN22 genes/human/PTPN22/PTPN22-ai-review.yaml PTPN22 Q9Y2R2 NCBITaxon:9606 Homo sapiens 18 R-HSA-8852200 Inactivation of LCK by PTPN22 yes reactome/R-HSA-8852200.md Inactivation of LCK by PTPN22 Homo sapiens GO:0004725 protein tyrosine phosphatase activity TAS enables MF false ACCEPT TAS from Reactome (Inactivation of LCK by PTPN22). This reflects the specific dephosphorylation of LCK by PTPN22 in the context of TCR signaling. reaction_mf_exact accepted_mf 1 human PTPN22 genes/human/PTPN22/PTPN22-ai-review.yaml PTPN22 Q9Y2R2 NCBITaxon:9606 Homo sapiens 19 R-HSA-8855381 PTPN22 dephosphorylates ZAP70 yes reactome/R-HSA-8855381.md PTPN22 dephosphorylates ZAP70 Homo sapiens GO:0004725 protein tyrosine phosphatase activity TAS enables MF false ACCEPT TAS from Reactome (PTPN22 dephosphorylates ZAP70). This reflects the specific dephosphorylation of ZAP-70 at Tyr-493 by PTPN22. reaction_mf_exact accepted_mf 1 human PTPN6 genes/human/PTPN6/PTPN6-ai-review.yaml PTPN6 P29350 NCBITaxon:9606 Homo sapiens 70 R-HSA-389758 Dephosphorylation of CD3-zeta by PD-1 bound phosphatases yes reactome/R-HSA-389758.md Dephosphorylation of CD3-zeta by PD-1 bound phosphatases Homo sapiens GO:0004725 protein tyrosine phosphatase activity TAS enables MF false ACCEPT protein tyrosine phosphatase activity is the core catalytic activity of PTPN6/SHP-1. reaction_mf_exact accepted_mf multi_gene_reactome_id 2 human PTPN6 genes/human/PTPN6/PTPN6-ai-review.yaml PTPN6 P29350 NCBITaxon:9606 Homo sapiens 71 R-HSA-914036 SHP1 and SHP2 dephosphorylate Y628 of IL3RB yes reactome/R-HSA-914036.md SHP1 and SHP2 dephosphorylate Y628 of IL3RB Homo sapiens GO:0004725 protein tyrosine phosphatase activity TAS enables MF false ACCEPT protein tyrosine phosphatase activity is the core catalytic activity of PTPN6/SHP-1. reaction_mf_exact accepted_mf multi_gene_reactome_id 2 human PTPN6 genes/human/PTPN6/PTPN6-ai-review.yaml PTPN6 P29350 NCBITaxon:9606 Homo sapiens 72 R-HSA-9701507 PTPN6 dephosphorylates JAK3 yes reactome/R-HSA-9701507.md PTPN6 dephosphorylates JAK3 Homo sapiens GO:0004725 protein tyrosine phosphatase activity TAS enables MF false ACCEPT protein tyrosine phosphatase activity is the core catalytic activity of PTPN6/SHP-1. reaction_mf_exact accepted_mf 1 human PTPN6 genes/human/PTPN6/PTPN6-ai-review.yaml PTPN6 P29350 NCBITaxon:9606 Homo sapiens 73 R-HSA-997314 Dephosphorylation of JAK1 by SHP1 yes reactome/R-HSA-997314.md Dephosphorylation of JAK1 by SHP1 Homo sapiens GO:0004725 protein tyrosine phosphatase activity TAS enables MF false ACCEPT protein tyrosine phosphatase activity is the core catalytic activity of PTPN6/SHP-1. reaction_mf_exact accepted_mf 1 human RBX1 genes/human/RBX1/RBX1-ai-review.yaml RBX1 P62877 NCBITaxon:9606 Homo sapiens 9 R-HSA-69598 Ubiquitination of phosphorylated CDC25A yes reactome/R-HSA-69598.md Ubiquitination of phosphorylated CDC25A Homo sapiens GO:0004842 ubiquitin-protein transferase activity TAS enables MF false ACCEPT RBX1 supplies the RING activity that transfers ubiquitin from a charged E2 onto CRL-recruited substrates; the annotation is the parent of the more precise ubiquitin protein ligase activity (GO:0061630) also annotated here. reaction_mf_exact accepted_mf 1 human RBX1 genes/human/RBX1/RBX1-ai-review.yaml RBX1 P62877 NCBITaxon:9606 Homo sapiens 262 R-HSA-8952618 AcM-UBE2M transfers NEDD8 to CRL1 E3 ubiquitin ligase complex yes reactome/R-HSA-8952618.md AcM-UBE2M transfers NEDD8 to CRL1 E3 ubiquitin ligase complex Homo sapiens GO:0019788 NEDD8 transferase activity TAS enables MF false ACCEPT RBX1 is the RING E3 of the cullin neddylation reaction, binding UBE2M~NEDD8 and transferring NEDD8 to the conserved lysine of CUL1-CUL4. reaction_mf_exact accepted_mf 1 human RBX1 genes/human/RBX1/RBX1-ai-review.yaml RBX1 P62877 NCBITaxon:9606 Homo sapiens 263 R-HSA-8952626 AcM-UBE2M transfers NEDD8 to CRL2 E3 ubiquitin ligase complex yes reactome/R-HSA-8952626.md AcM-UBE2M transfers NEDD8 to CRL2 E3 ubiquitin ligase complex Homo sapiens GO:0019788 NEDD8 transferase activity TAS enables MF false ACCEPT RBX1 is the RING E3 of the cullin neddylation reaction, binding UBE2M~NEDD8 and transferring NEDD8 to the conserved lysine of CUL1-CUL4. reaction_mf_exact accepted_mf 1 human RBX1 genes/human/RBX1/RBX1-ai-review.yaml RBX1 P62877 NCBITaxon:9606 Homo sapiens 264 R-HSA-8952631 AcM-UBE2M transfers NEDD8 to CRL3 E3 ubiquitin ligase complex yes reactome/R-HSA-8952631.md AcM-UBE2M transfers NEDD8 to CRL3 E3 ubiquitin ligase complex Homo sapiens GO:0019788 NEDD8 transferase activity TAS enables MF false ACCEPT RBX1 is the RING E3 of the cullin neddylation reaction, binding UBE2M~NEDD8 and transferring NEDD8 to the conserved lysine of CUL1-CUL4. reaction_mf_exact accepted_mf 1 human RBX1 genes/human/RBX1/RBX1-ai-review.yaml RBX1 P62877 NCBITaxon:9606 Homo sapiens 265 R-HSA-8952638 AcM-UBE2M transfers NEDD8 to CRL4 E3 ubiquitin ligase complex yes reactome/R-HSA-8952638.md AcM-UBE2M transfers NEDD8 to CRL4 E3 ubiquitin ligase complex Homo sapiens GO:0019788 NEDD8 transferase activity TAS enables MF false ACCEPT RBX1 is the RING E3 of the cullin neddylation reaction, binding UBE2M~NEDD8 and transferring NEDD8 to the conserved lysine of CUL1-CUL4. reaction_mf_exact accepted_mf 1 human RBX1 genes/human/RBX1/RBX1-ai-review.yaml RBX1 P62877 NCBITaxon:9606 Homo sapiens 453 R-HSA-1504190 DVL is ubiquitinated by CUL3:KLHL12:RBX1 yes reactome/R-HSA-1504190.md DVL is ubiquitinated by CUL3:KLHL12:RBX1 Homo sapiens GO:0061630 ubiquitin protein ligase activity TAS enables MF false ACCEPT Core molecular function: RBX1 is the RING E3 of cullin-RING ligases, binding ubiquitin-charged E2s (CDC34/UBE2R, UBE2D) and positioning them for transfer onto substrates recruited by the cullin's receptor module. reaction_mf_exact accepted_mf 1 human RBX1 genes/human/RBX1/RBX1-ai-review.yaml RBX1 P62877 NCBITaxon:9606 Homo sapiens 454 R-HSA-5635856 SPOP:CUL3:RBX1 ubiquitinates GLI2,3 yes reactome/R-HSA-5635856.md SPOP:CUL3:RBX1 ubiquitinates GLI2,3 Homo sapiens GO:0061630 ubiquitin protein ligase activity TAS enables MF false ACCEPT Core molecular function: RBX1 is the RING E3 of cullin-RING ligases, binding ubiquitin-charged E2s (CDC34/UBE2R, UBE2D) and positioning them for transfer onto substrates recruited by the cullin's receptor module. reaction_mf_exact accepted_mf 1 human RBX1 genes/human/RBX1/RBX1-ai-review.yaml RBX1 P62877 NCBITaxon:9606 Homo sapiens 455 R-HSA-5658424 KBTBD7:CUL3:RBX1 ubiquitinates NF1 yes reactome/R-HSA-5658424.md KBTBD7:CUL3:RBX1 ubiquitinates NF1 Homo sapiens GO:0061630 ubiquitin protein ligase activity TAS enables MF false ACCEPT Core molecular function: RBX1 is the RING E3 of cullin-RING ligases, binding ubiquitin-charged E2s (CDC34/UBE2R, UBE2D) and positioning them for transfer onto substrates recruited by the cullin's receptor module. reaction_mf_exact accepted_mf 1 human RBX1 genes/human/RBX1/RBX1-ai-review.yaml RBX1 P62877 NCBITaxon:9606 Homo sapiens 456 R-HSA-9755505 KEAP1:NEDD8-CUL3:RBX1 complex ubiquitinates NFE2L2 yes reactome/R-HSA-9755505.md KEAP1:NEDD8-CUL3:RBX1 complex ubiquitinates NFE2L2 Homo sapiens GO:0061630 ubiquitin protein ligase activity TAS enables MF false ACCEPT Core molecular function: RBX1 is the RING E3 of cullin-RING ligases, binding ubiquitin-charged E2s (CDC34/UBE2R, UBE2D) and positioning them for transfer onto substrates recruited by the cullin's receptor module. reaction_mf_exact accepted_mf 1 human RBX1 genes/human/RBX1/RBX1-ai-review.yaml RBX1 P62877 NCBITaxon:9606 Homo sapiens 457 R-HSA-9759172 KEAP1:CUL3:RBX1 ubiquitinates p-S349 SQSTM1 oligomer yes reactome/R-HSA-9759172.md KEAP1:CUL3:RBX1 ubiquitinates p-S349 SQSTM1 oligomer Homo sapiens GO:0061630 ubiquitin protein ligase activity TAS enables MF false ACCEPT Core molecular function: RBX1 is the RING E3 of cullin-RING ligases, binding ubiquitin-charged E2s (CDC34/UBE2R, UBE2D) and positioning them for transfer onto substrates recruited by the cullin's receptor module. reaction_mf_exact accepted_mf 1 human RBX1 genes/human/RBX1/RBX1-ai-review.yaml RBX1 P62877 NCBITaxon:9606 Homo sapiens 458 R-HSA-9762091 NFE2L2 is ubiquitinated by BTRC:SKP1:CUL1:RBX1 yes reactome/R-HSA-9762091.md NFE2L2 is ubiquitinated by BTRC:SKP1:CUL1:RBX1 Homo sapiens GO:0061630 ubiquitin protein ligase activity TAS enables MF false ACCEPT Core molecular function: RBX1 is the RING E3 of cullin-RING ligases, binding ubiquitin-charged E2s (CDC34/UBE2R, UBE2D) and positioning them for transfer onto substrates recruited by the cullin's receptor module. reaction_mf_exact accepted_mf 1 human RBX1 genes/human/RBX1/RBX1-ai-review.yaml RBX1 P62877 NCBITaxon:9606 Homo sapiens 459 R-HSA-9766645 CUL3:RBX1 ubiquitinates KEAP1 yes reactome/R-HSA-9766645.md CUL3:RBX1 ubiquitinates KEAP1 Homo sapiens GO:0061630 ubiquitin protein ligase activity TAS enables MF false ACCEPT Core molecular function: RBX1 is the RING E3 of cullin-RING ligases, binding ubiquitin-charged E2s (CDC34/UBE2R, UBE2D) and positioning them for transfer onto substrates recruited by the cullin's receptor module. reaction_mf_exact accepted_mf 1 human RDH11 genes/human/RDH11/RDH11-ai-review.yaml RDH11 Q8TC12 NCBITaxon:9606 Homo sapiens 15 R-HSA-5419165 RDH11,14,DHRS3,DRHS4 reduce atRAL to atROL yes reactome/R-HSA-5419165.md RDH11,14,DHRS3,DRHS4 reduce atRAL to atROL Homo sapiens GO:0052650 all-trans-retinol dehydrogenase (NADP+) activity TAS enables MF false ACCEPT Core NADP-dependent all-trans-retinol dehydrogenase activity, Reactome TAS (RDH11 et al. reduce atRAL to atROL). Correct and well-supported; redundant with the EXP/IDA/IEA annotations to the same term. reaction_mf_exact accepted_mf multi_gene_reactome_id 2 human RDH11 genes/human/RDH11/RDH11-ai-review.yaml RDH11 Q8TC12 NCBITaxon:9606 Homo sapiens 16 R-HSA-74872 RDH10,11 oxidise 11cROL to 11cRAL yes reactome/R-HSA-74872.md RDH10,11 oxidise 11cROL to 11cRAL Homo sapiens GO:0052650 all-trans-retinol dehydrogenase (NADP+) activity TAS enables MF false ACCEPT Core NADP-dependent all-trans-retinol dehydrogenase activity, Reactome TAS. Correct; redundant with the other annotations to this term. reaction_mf_exact accepted_mf 1 human RDH12 genes/human/RDH12/RDH12-ai-review.yaml RDH12 Q96NR8 NCBITaxon:9606 Homo sapiens 15 R-HSA-2464822 RDH12 reduces atRAL to atROL yes reactome/R-HSA-2464822.md RDH12 reduces atRAL to atROL Homo sapiens GO:0052650 all-trans-retinol dehydrogenase (NADP+) activity TAS enables MF false ACCEPT Core molecular function, consistent with the experimental annotations. Reactome models RDH12 reducing all-trans-retinal to all-trans-retinol. reaction_mf_exact accepted_mf 1 human RDH12 genes/human/RDH12/RDH12-ai-review.yaml RDH12 Q96NR8 NCBITaxon:9606 Homo sapiens 16 R-HSA-2466861 Defective RDH12 does not reduce atRAL to atROL yes reactome/R-HSA-2466861.md Defective RDH12 does not reduce atRAL to atROL Homo sapiens GO:0052650 all-trans-retinol dehydrogenase (NADP+) activity TAS enables MF false ACCEPT "Core molecular function from the Reactome ""defective RDH12"" (LCA13/RP53) reaction; the wild-type activity is the NADP(H)-dependent atRAL->atROL reduction." reaction_mf_exact accepted_mf reactome_disease_event 1 human RFK genes/human/RFK/RFK-ai-review.yaml RFK Q969G6 NCBITaxon:9606 Homo sapiens 12 R-HSA-196964 RFK:Mg2+ phosphorylates RIB yes reactome/R-HSA-196964.md RFK:Mg2+ phosphorylates RIB Homo sapiens GO:0008531 riboflavin kinase activity TAS enables MF false ACCEPT "Author/curator (TAS) annotation from the Reactome reaction ""RFK:Mg2+ phosphorylates RIB"", describing RFK phosphorylating riboflavin to FMN using zinc or magnesium. Correct core molecular function. Accept." reaction_mf_exact accepted_mf 1 human RNF185 genes/human/RNF185/RNF185-ai-review.yaml RNF185 Q96GF1 NCBITaxon:9606 Homo sapiens 23 R-HSA-8866546 RNF5 and RNF185 ubiquitinate misfolded CFTR yes reactome/R-HSA-8866546.md RNF5 and RNF185 ubiquitinate misfolded CFTR Homo sapiens GO:0061630 ubiquitin protein ligase activity TAS enables MF false ACCEPT Reactome curation of RNF185 (with RNF5) ubiquitinating misfolded CFTR; captures the core ligase activity. reaction_mf_exact accepted_mf multi_gene_reactome_id 2 human RNF185 genes/human/RNF185/RNF185-ai-review.yaml RNF185 Q96GF1 NCBITaxon:9606 Homo sapiens 24 R-HSA-8867288 OS9:SEL1:ERAD E3 ligase:DERL2 ubiquitinates unfolded protein:(GlcNAc)2 (Man)9-5 yes reactome/R-HSA-8867288.md OS9:SEL1:ERAD E3 ligase:DERL2 ubiquitinates unfolded protein:(GlcNAc)2 (Man)9-5 Homo sapiens GO:0061630 ubiquitin protein ligase activity TAS enables MF false ACCEPT Reactome curation of the ERAD E3 ligase ubiquitinating an unfolded glycoprotein substrate; captures the core ligase activity. reaction_mf_exact accepted_mf multi_gene_reactome_id 4 human RNF41 genes/human/RNF41/RNF41-ai-review.yaml RNF41 Q9H4P4 NCBITaxon:9606 Homo sapiens 22 R-HSA-1358789 Self-ubiquitination of RNF41 yes reactome/R-HSA-1358789.md Self-ubiquitination of RNF41 Homo sapiens GO:0004842 ubiquitin-protein transferase activity TAS enables MF false ACCEPT Reactome curation (self-ubiquitination of RNF41) of ubiquitin-protein transferase activity, the core catalytic molecular function. reaction_mf_exact accepted_mf 1 human RNF5 genes/human/RNF5/RNF5-ai-review.yaml RNF5 Q99942 NCBITaxon:9606 Homo sapiens 31 R-HSA-8866546 RNF5 and RNF185 ubiquitinate misfolded CFTR yes reactome/R-HSA-8866546.md RNF5 and RNF185 ubiquitinate misfolded CFTR Homo sapiens GO:0061630 ubiquitin protein ligase activity TAS enables MF false ACCEPT Reactome curation of RNF5 (with RNF185) ubiquitinating misfolded CFTR; captures the core ligase activity. reaction_mf_exact accepted_mf multi_gene_reactome_id 2 human RNF5 genes/human/RNF5/RNF5-ai-review.yaml RNF5 Q99942 NCBITaxon:9606 Homo sapiens 32 R-HSA-8867288 OS9:SEL1:ERAD E3 ligase:DERL2 ubiquitinates unfolded protein:(GlcNAc)2 (Man)9-5 yes reactome/R-HSA-8867288.md OS9:SEL1:ERAD E3 ligase:DERL2 ubiquitinates unfolded protein:(GlcNAc)2 (Man)9-5 Homo sapiens GO:0061630 ubiquitin protein ligase activity TAS enables MF false ACCEPT Reactome curation of the ERAD E3 ligase ubiquitinating an unfolded glycoprotein substrate; captures the core ligase activity. reaction_mf_exact accepted_mf multi_gene_reactome_id 4 human RPE65 genes/human/RPE65/RPE65-ai-review.yaml RPE65 Q16518 NCBITaxon:9606 Homo sapiens 27 R-HSA-2453833 RPE65 isomero-hydrolyses atREs to 11cROL yes reactome/R-HSA-2453833.md RPE65 isomero-hydrolyses atREs to 11cROL Homo sapiens GO:0052884 all-trans-retinyl-palmitate hydrolase, 11-cis retinol forming activity TAS enables MF false ACCEPT "Reactome TAS annotation of the palmitate-specific isomerohydrolase reaction, matching the curated ""RPE65 isomero-hydrolyses atREs to 11cROL"" reaction. Correct core molecular function." reaction_mf_exact accepted_mf 1 human RPIA genes/human/RPIA/RPIA-ai-review.yaml RPIA P49247 NCBITaxon:9606 Homo sapiens 25 R-HSA-5660013 Defective RPIA does not isomerize RU5P to R5P yes reactome/R-HSA-5660013.md Defective RPIA does not isomerize RU5P to R5P Homo sapiens GO:0004751 ribose-5-phosphate isomerase activity TAS enables MF false ACCEPT Reactome traceable-author annotation of the core isomerase activity (in the context of a reaction whose defective form underlies RPIA deficiency). Correct core molecular function. reaction_mf_exact accepted_mf reactome_disease_event 1 human RPIA genes/human/RPIA/RPIA-ai-review.yaml RPIA P49247 NCBITaxon:9606 Homo sapiens 26 R-HSA-5660015 Defective RPIA does not isomerize R5P to RU5P yes reactome/R-HSA-5660015.md Defective RPIA does not isomerize R5P to RU5P Homo sapiens GO:0004751 ribose-5-phosphate isomerase activity TAS enables MF false ACCEPT Reactome traceable-author annotation of the core isomerase activity. Correct core molecular function. reaction_mf_exact accepted_mf reactome_disease_event 1 human SAT1 genes/human/SAT1/SAT1-ai-review.yaml SAT1 P21673 NCBITaxon:9606 Homo sapiens 22 R-HSA-351207 Spermine => N-acetylated spermine yes reactome/R-HSA-351207.md Spermine => N-acetylated spermine Homo sapiens GO:0004145 diamine N-acetyltransferase activity TAS enables MF false ACCEPT "TAS annotation (Reactome ""Spermine => N-acetylated spermine"") of the core catalytic activity: SAT1 acetylates spermine to N-acetylspermine, the rate-limiting step of polyamine catabolism. Accept as a core molecular function." reaction_mf_exact accepted_mf 1 human SAT1 genes/human/SAT1/SAT1-ai-review.yaml SAT1 P21673 NCBITaxon:9606 Homo sapiens 23 R-HSA-351208 Spermidine => N-acetylated spermidine yes reactome/R-HSA-351208.md Spermidine => N-acetylated spermidine Homo sapiens GO:0004145 diamine N-acetyltransferase activity TAS enables MF false ACCEPT "TAS annotation (Reactome ""Spermidine => N-acetylated spermidine"") of the core catalytic activity: SAT1 acetylates spermidine to N-acetylspermidine. Accept as a core molecular function." reaction_mf_exact accepted_mf 1 human SCP2 genes/human/SCP2/SCP2-ai-review.yaml SCP2 P22307 NCBITaxon:9606 Homo sapiens 35 R-HSA-192341 Thiolysis of 3alpha,7alpha,12alpha-trihydroxy-5beta-cholan-24-one-CoA yields choloyl-CoA (3alpha,7alpha,12alpha-trihydroxy-5beta-cholan-24-one-CoA) and propionyl CoA yes reactome/R-HSA-192341.md Thiolysis of 3alpha,7alpha,12alpha-trihydroxy-5beta-cholan-24-one-CoA yields choloyl-CoA (3alpha,7alpha,12alpha-trihydroxy-5beta-cholan-24-one-CoA) and propionyl CoA Homo sapiens GO:0050632 propionyl-CoA C2-trimethyltridecanoyltransferase activity TAS enables MF false ACCEPT Reactome TAS for the SCPx-catalyzed thiolytic step (trihydroxy-cholestanoyl thiolysis to choloyl-CoA + propionyl-CoA). Supports the core thiolase MF. reaction_mf_exact accepted_mf 1 human SCP2 genes/human/SCP2/SCP2-ai-review.yaml SCP2 P22307 NCBITaxon:9606 Homo sapiens 36 R-HSA-193533 Thiolysis of 3alpha,7alpha-dihydroxy-5beta-cholan-24-one-CoA yields chenodeoxycholoyl-CoA (3alpha,7alpha-dihydroxy-5beta-cholan-24-one-CoA) and propionyl CoA yes reactome/R-HSA-193533.md Thiolysis of 3alpha,7alpha-dihydroxy-5beta-cholan-24-one-CoA yields chenodeoxycholoyl-CoA (3alpha,7alpha-dihydroxy-5beta-cholan-24-one-CoA) and propionyl CoA Homo sapiens GO:0050632 propionyl-CoA C2-trimethyltridecanoyltransferase activity TAS enables MF false ACCEPT Reactome TAS for the analogous SCPx thiolytic step in chenodeoxycholoyl-CoA formation. Supports the core thiolase MF. reaction_mf_exact accepted_mf 1 human SDHA genes/human/SDHA/SDHA-ai-review.yaml SDHA P31040 NCBITaxon:9606 Homo sapiens 38 R-HSA-9854672 FAD attaches to SDHA yes reactome/R-HSA-9854672.md FAD attaches to SDHA Homo sapiens GO:0071949 FAD binding TAS enables MF false ACCEPT TAS annotation from Reactome for FAD binding. The Reactome entry R-HSA-9854672 describes the covalent attachment of FAD to SDHA. GO:0071949 (FAD binding) specifically refers to binding the oxidized form of FAD, which is appropriate for SDHA. reaction_mf_exact accepted_mf 1 human SEC11A genes/human/SEC11A/SEC11A-ai-review.yaml SEC11A P67812 NCBITaxon:9606 Homo sapiens 13 R-HSA-9829030 Nascent F signal peptide is cleaved at ER membrane yes reactome/R-HSA-9829030.md Nascent F signal peptide is cleaved at ER membrane Homo sapiens GO:0004252 serine-type endopeptidase activity TAS enables MF false ACCEPT Reactome curation of SEC11A serine-endopeptidase activity (cleavage of a viral fusion-protein signal peptide at the ER membrane); consistent with its core catalytic function. reaction_mf_exact accepted_mf 1 human SEC11A genes/human/SEC11A/SEC11A-ai-review.yaml SEC11A P67812 NCBITaxon:9606 Homo sapiens 14 R-HSA-9932162 Removal of CDH1 signal peptide yes reactome/R-HSA-9932162.md Removal of CDH1 signal peptide Homo sapiens GO:0004252 serine-type endopeptidase activity TAS enables MF false ACCEPT Reactome curation of SEC11A serine-endopeptidase activity (removal of the CDH1 signal peptide); consistent with its core catalytic function. reaction_mf_exact accepted_mf 1 human SEPHS2 genes/human/SEPHS2/SEPHS2-ai-review.yaml SEPHS2 Q99611 NCBITaxon:9606 Homo sapiens 10 R-HSA-8959510 SEPHS2 phosphorylates H2Se to form SELP no GO:0004756 selenide, water dikinase activity TAS enables MF false ACCEPT Reactome (TAS) annotation of the core catalytic function, representing the reaction in which SEPHS2 converts selenide (H2Se) to selenophosphate. Concordant with UniProt and the human enzymology. Accept. reaction_mf_exact accepted_mf uncached_reactome 1 human SGMS1 genes/human/SGMS1/SGMS1-ai-review.yaml SGMS1 Q86VZ5 NCBITaxon:9606 Homo sapiens 17 R-HSA-429798 SGMS1 transfers phosphocholine onto ceramide yes reactome/R-HSA-429798.md SGMS1 transfers phosphocholine onto ceramide Homo sapiens GO:0033188 sphingomyelin synthase activity TAS enables MF false ACCEPT Reactome (TAS) assignment of sphingomyelin synthase activity, matching the reaction 'SGMS1 transfers phosphocholine onto ceramide'. Correct core function. reaction_mf_exact accepted_mf 1 human SGMS2 genes/human/SGMS2/SGMS2-ai-review.yaml SGMS2 Q8NHU3 NCBITaxon:9606 Homo sapiens 19 R-HSA-429786 SGMS2 transfers phosphocholine onto ceramide yes reactome/R-HSA-429786.md SGMS2 transfers phosphocholine onto ceramide Homo sapiens GO:0033188 sphingomyelin synthase activity TAS enables MF false ACCEPT Reactome traceable-author-statement for the SGMS2 reaction (transfers phosphocholine onto ceramide), i.e. the core sphingomyelin synthase activity. reaction_mf_exact accepted_mf 1 human SGPL1 genes/human/SGPL1/SGPL1-ai-review.yaml SGPL1 O95470 NCBITaxon:9606 Homo sapiens 14 R-HSA-428681 PXLP-SGPL1 cleaves sphingoid-1-phosphates yes reactome/R-HSA-428681.md PXLP-SGPL1 cleaves sphingoid-1-phosphates Homo sapiens GO:0008117 sphinganine-1-phosphate aldolase activity TAS enables MF false ACCEPT Reactome traceable assertion for the S1P/sphinganine 1-phosphate lyase reaction. Core molecular function, consistent with experimental data. reaction_mf_exact accepted_mf 1 human SGPP1 genes/human/SGPP1/SGPP1-ai-review.yaml SGPP1 Q9BX95 NCBITaxon:9606 Homo sapiens 18 R-HSA-428664 SGPP1,2 dephosphorylate sphingoid-1-phosphates yes reactome/R-HSA-428664.md SGPP1,2 dephosphorylate sphingoid-1-phosphates Homo sapiens GO:0042392 sphingosine-1-phosphate phosphatase activity TAS enables MF false ACCEPT Reactome traceable-author-statement for the S1P phosphatase reaction (SGPP1,2 dephosphorylate sphingoid-1-phosphates). Correct and consistent with the experimental annotations to the same core activity. reaction_mf_exact accepted_mf multi_gene_reactome_id 2 human SGPP2 genes/human/SGPP2/SGPP2-ai-review.yaml SGPP2 Q8IWX5 NCBITaxon:9606 Homo sapiens 14 R-HSA-428664 SGPP1,2 dephosphorylate sphingoid-1-phosphates yes reactome/R-HSA-428664.md SGPP1,2 dephosphorylate sphingoid-1-phosphates Homo sapiens GO:0042392 sphingosine-1-phosphate phosphatase activity TAS enables MF false ACCEPT Reactome traceable-author annotation to the core sphingosine-1-phosphate phosphatase activity, modeling SGPP1/2 dephosphorylation of cytosolic S1P, dihydro-S1P and phyto-S1P at the ER membrane. reaction_mf_exact accepted_mf multi_gene_reactome_id 2 human SIAH1 genes/human/SIAH1/SIAH1-ai-review.yaml SIAH1 Q8IUQ4 NCBITaxon:9606 Homo sapiens 34 R-HSA-5660753 SIAH1:UBE2L6:Ubiquitin ubiquitinates SNCA yes reactome/R-HSA-5660753.md SIAH1:UBE2L6:Ubiquitin ubiquitinates SNCA Homo sapiens GO:0004842 ubiquitin-protein transferase activity TAS enables MF false ACCEPT Reactome curation (SIAH1:UBE2L6:Ubiquitin ubiquitinates SNCA) of ubiquitin-protein transferase activity, the core catalytic molecular function. reaction_mf_exact accepted_mf 1 human SIAH1 genes/human/SIAH1/SIAH1-ai-review.yaml SIAH1 Q8IUQ4 NCBITaxon:9606 Homo sapiens 35 R-HSA-5667107 SIAH1, SIAH2 ubiquitinate SNCAIP yes reactome/R-HSA-5667107.md SIAH1, SIAH2 ubiquitinate SNCAIP Homo sapiens GO:0004842 ubiquitin-protein transferase activity TAS enables MF false ACCEPT Reactome curation (SIAH1, SIAH2 ubiquitinate SNCAIP) of ubiquitin-protein transferase activity, the core catalytic molecular function. reaction_mf_exact accepted_mf 1 human SIRT1 genes/human/SIRT1/SIRT1-ai-review.yaml SIRT1 Q96EB6 NCBITaxon:9606 Homo sapiens 161 R-HSA-3371467 SIRT1 deacetylates HSF1 yes reactome/R-HSA-3371467.md SIRT1 deacetylates HSF1 Homo sapiens GO:0034979 NAD-dependent protein lysine deacetylase activity TAS enables MF false ACCEPT SIRT1 functions as a NAD-dependent deacetylase in the HSF1 heat shock pathway. reaction_mf_exact accepted_mf 1 human SIRT1 genes/human/SIRT1/SIRT1-ai-review.yaml SIRT1 Q96EB6 NCBITaxon:9606 Homo sapiens 162 R-HSA-9620532 SIRT1,SIRT3 deacetylate FOXO3 yes reactome/R-HSA-9620532.md SIRT1,SIRT3 deacetylate FOXO3 Homo sapiens GO:0034979 NAD-dependent protein lysine deacetylase activity TAS enables MF false ACCEPT SIRT1 deacetylates FOXO3 as an NAD-dependent histone deacetylase. reaction_mf_exact accepted_mf 1 human SIRT1 genes/human/SIRT1/SIRT1-ai-review.yaml SIRT1 Q96EB6 NCBITaxon:9606 Homo sapiens 174 R-HSA-9825772 SIRT1 deacetylates HINT1 dimer yes reactome/R-HSA-9825772.md SIRT1 deacetylates HINT1 dimer Homo sapiens GO:0033558 protein lysine deacetylase activity TAS enables MF false ACCEPT SIRT1 deacetylates HINT1, consistent with protein lysine deacetylase activity. reaction_mf_exact accepted_mf 1 human SIRT2 genes/human/SIRT2/SIRT2-ai-review.yaml SIRT2 Q8IXJ6 NCBITaxon:9606 Homo sapiens 86 R-HSA-9667952 Reactome pathway: SIRT2 deacetylates ANKLE2 yes reactome/R-HSA-9667952.md ANKLE2 is deacetylated by SIRT2 Homo sapiens GO:0034979 NAD-dependent protein lysine deacetylase activity TAS enables MF false ACCEPT SIRT2 deacetylates ANKLE2 in Reactome pathway. reaction_mf_exact accepted_mf 1 human SLC14A1 genes/human/SLC14A1/SLC14A1-ai-review.yaml SLC14A1 Q13336 NCBITaxon:9606 Homo sapiens 18 R-HSA-444126 HUT2 and HUT11 mediate urea transport in kidney and erythrocytes respectively yes reactome/R-HSA-444126.md HUT2 and HUT11 mediate urea transport in kidney and erythrocytes respectively Homo sapiens GO:0015204 urea transmembrane transporter activity TAS enables MF false ACCEPT The Reactome pathway correctly describes HUT11 (SLC14A1) as an erythrocyte-specific urea transporter mediating rapid urea movement across cell membranes. reaction_mf_exact accepted_mf 1 human SLC19A2 genes/human/SLC19A2/SLC19A2-ai-review.yaml SLC19A2 O60779 NCBITaxon:9606 Homo sapiens 25 R-HSA-199626 SLC19A2/3 transport extracellular THMN to cytosol yes reactome/R-HSA-199626.md SLC19A2/3 transport extracellular THMN to cytosol Homo sapiens GO:0015234 thiamine transmembrane transporter activity TAS enables MF false ACCEPT Reactome models SLC19A2 (THTR1) as a plasma-membrane transporter of extracellular thiamine to the cytosol; matches the core molecular function. reaction_mf_exact accepted_mf multi_gene_reactome_id 2 human SLC19A3 genes/human/SLC19A3/SLC19A3-ai-review.yaml SLC19A3 Q9BZV2 NCBITaxon:9606 Homo sapiens 19 R-HSA-199626 SLC19A2/3 transport extracellular THMN to cytosol yes reactome/R-HSA-199626.md SLC19A2/3 transport extracellular THMN to cytosol Homo sapiens GO:0015234 thiamine transmembrane transporter activity TAS enables MF false ACCEPT Reactome traceable assertion of the core molecular function: SLC19A3 mediates transport of extracellular thiamine into the cytosol at the plasma membrane. Core MF; accept. reaction_mf_exact accepted_mf multi_gene_reactome_id 2 human SLC22A5 genes/human/SLC22A5/SLC22A5-ai-review.yaml SLC22A5 O76082 NCBITaxon:9606 Homo sapiens 58 R-HSA-549297 SLC22A4, 5,15,16 cotransport CAR, Na+ from extracellular region to cytosol no GO:0015226 carnitine transmembrane transporter activity TAS enables MF false ACCEPT Reactome assertion that SLC22A5 (with SLC22A4/15/16) cotransports carnitine and Na+ from extracellular region to cytosol. Core molecular function. reaction_mf_exact accepted_mf uncached_reactome 1 human SLC22A5 genes/human/SLC22A5/SLC22A5-ai-review.yaml SLC22A5 O76082 NCBITaxon:9606 Homo sapiens 59 R-HSA-5625674 Defective SLC22A5 does not cotransport CAR, Na+ from extracellular region to cytosol no GO:0015226 carnitine transmembrane transporter activity TAS enables MF false ACCEPT Reactome assertion (defective SLC22A5 does not cotransport carnitine/Na+) referencing the normal carnitine transporter activity of OCTN2. Core molecular function. reaction_mf_exact accepted_mf reactome_disease_event|uncached_reactome 1 human SLC25A15 genes/human/SLC25A15/SLC25A15-ai-review.yaml SLC25A15 Q9Y619 NCBITaxon:9606 Homo sapiens 10 R-HSA-9956519 SLC25A15 variants don't translocate ornithine and citrulline no GO:0000064 L-ornithine transmembrane transporter activity TAS enables MF false ACCEPT Reactome-traceable assignment of the core L-ornithine transmembrane transporter activity. reaction_mf_exact accepted_mf uncached_reactome 1 human SLC25A19 genes/human/SLC25A19/SLC25A19-ai-review.yaml SLC25A19 Q9HC21 NCBITaxon:9606 Homo sapiens 24 R-HSA-8875838 SLC25A19 transports ThDP from cytosol to mitochondrial matrix yes reactome/R-HSA-8875838.md SLC25A19 transports ThDP from cytosol to mitochondrial matrix Homo sapiens GO:0090422 thiamine pyrophosphate transmembrane transporter activity TAS enables MF false ACCEPT Reactome (TAS) assertion of the core TPP transporter activity, describing transport of ThDP from cytosol to the mitochondrial matrix. Consistent with the experimental evidence; supports the core MF. reaction_mf_exact accepted_mf 1 human SLC25A20 genes/human/SLC25A20/SLC25A20-ai-review.yaml SLC25A20 O43772 NCBITaxon:9606 Homo sapiens 12 R-HSA-9971094 SLC25A20 exports excess acyl-CAR no GO:0005476 carnitine:O-acyl-L-carnitine antiporter activity TAS enables MF false ACCEPT Reactome traceable assertion of the carnitine:acylcarnitine antiporter activity; duplicate of the EXP call and equally correct. reaction_mf_exact accepted_mf uncached_reactome 1 human SLC25A21 genes/human/SLC25A21/SLC25A21-ai-review.yaml SLC25A21 Q9BQT8 NCBITaxon:9606 Homo sapiens 10 R-HSA-372480 2-oxoglutarate [mitochondrial matrix] + 2-oxoadipate [cytosol] <=> 2-oxoglutarate [cytosol] + 2-oxoadipate [mitochondrial matrix] yes reactome/R-HSA-372480.md 2-oxoglutarate [mitochondrial matrix] + 2-oxoadipate [cytosol] <=> 2-oxoglutarate [cytosol] + 2-oxoadipate [mitochondrial matrix] Homo sapiens GO:0015139 alpha-ketoglutarate transmembrane transporter activity TAS enables MF false ACCEPT Reactome captures the SLC25A21-mediated exchange reaction (2-oxoglutarate matrix + 2-oxoadipate cytosol <=> 2-oxoglutarate cytosol + 2-oxoadipate matrix). This is the same core transporter activity supported by the experimental annotations. Accepted. reaction_mf_exact accepted_mf 1 human SLC25A22 genes/human/SLC25A22/SLC25A22-ai-review.yaml SLC25A22 Q9H936 NCBITaxon:9606 Homo sapiens 20 R-HSA-8875623 SLC25A18,22 import L-Glu, H+ yes reactome/R-HSA-8875623.md SLC25A18,22 import L-Glu, H+ Homo sapiens GO:0005280 amino acid:proton symporter activity TAS enables MF false ACCEPT Reactome-asserted amino acid:proton symporter activity for the SLC25A18/SLC25A22 glutamate/H+ import reaction. Duplicate of the IDA call for the same core MF. Accept. reaction_mf_exact accepted_mf 1 human SLC27A5 genes/human/SLC27A5/SLC27A5-ai-review.yaml SLC27A5 Q9Y2P5 NCBITaxon:9606 Homo sapiens 23 R-HSA-159425 Cytosolic cholate and chenodeoxycholate are conjugated with Coenzyme A (SLC27A5 BACS) yes reactome/R-HSA-159425.md Cytosolic cholate and chenodeoxycholate are conjugated with Coenzyme A (SLC27A5 BACS) Homo sapiens GO:0004467 long-chain fatty acid-CoA ligase activity TAS enables MF false ACCEPT Reactome (TAS) molecular-function assignment for the BACS reaction conjugating cytosolic cholate/chenodeoxycholate with CoA on the ER membrane. Correct core catalytic function. reaction_mf_exact accepted_mf local_module_mf 1 bile_acid_synthesis_sidechain_conjugation:GO:0047747 cholate-CoA ligase activity human SLC27A5 genes/human/SLC27A5/SLC27A5-ai-review.yaml SLC27A5 Q9Y2P5 NCBITaxon:9606 Homo sapiens 24 R-HSA-192137 THCA is conjugated with Coenzyme A (SLC27A5 BACS) yes reactome/R-HSA-192137.md THCA is conjugated with Coenzyme A (SLC27A5 BACS) Homo sapiens GO:0004467 long-chain fatty acid-CoA ligase activity TAS enables MF false ACCEPT Reactome (TAS) molecular-function assignment for the BACS reaction conjugating the C27 precursor THCA with CoA. Consistent with the enzyme's demonstrated ability to activate THCA. reaction_mf_exact accepted_mf 1 human SLC27A5 genes/human/SLC27A5/SLC27A5-ai-review.yaml SLC27A5 Q9Y2P5 NCBITaxon:9606 Homo sapiens 25 R-HSA-193407 DHCA is conjugated with Coenzyme A (SLC27A5 BACS) yes reactome/R-HSA-193407.md DHCA is conjugated with Coenzyme A (SLC27A5 BACS) Homo sapiens GO:0004467 long-chain fatty acid-CoA ligase activity TAS enables MF false ACCEPT Reactome (TAS) molecular-function assignment for the BACS reaction conjugating DHCA with CoA. Consistent with the enzyme's acyl-CoA ligase activity toward bile-acid precursors. reaction_mf_exact accepted_mf 1 human SLC27A5 genes/human/SLC27A5/SLC27A5-ai-review.yaml SLC27A5 Q9Y2P5 NCBITaxon:9606 Homo sapiens 26 R-HSA-193711 3,7,24THCA is conjugated with Coenzyme A (SLC27A5 BACS) yes reactome/R-HSA-193711.md 3,7,24THCA is conjugated with Coenzyme A (SLC27A5 BACS) Homo sapiens GO:0004467 long-chain fatty acid-CoA ligase activity TAS enables MF false ACCEPT Reactome (TAS) molecular-function assignment for the BACS reaction conjugating 3,7,24-THCA with CoA. Consistent with the enzyme's acyl-CoA ligase activity. reaction_mf_exact accepted_mf 1 human SLC27A5 genes/human/SLC27A5/SLC27A5-ai-review.yaml SLC27A5 Q9Y2P5 NCBITaxon:9606 Homo sapiens 27 R-HSA-193766 Bile-acyl-CoA conjugation reaction (SLC27A5 BACS) no GO:0004467 long-chain fatty acid-CoA ligase activity TAS enables MF false ACCEPT Reactome (TAS) molecular-function assignment (bile-acyl-CoA conjugation reaction). The specific reaction id could not be verified in the current Reactome ContentService (returns 404, likely a retired/renumbered reaction), but the asserted ligase activity is the correct core function of SLC27A5. reaction_mf_exact accepted_mf uncached_reactome 1 human SLC35A1 genes/human/SLC35A1/SLC35A1-ai-review.yaml SLC35A1 P78382 NCBITaxon:9606 Homo sapiens 21 R-HSA-5651942 Defective SLC35A1 does not exchange CMP-Neu5Ac for CMP yes reactome/R-HSA-5651942.md Defective SLC35A1 does not exchange CMP-Neu5Ac for CMP Homo sapiens GO:0005456 CMP-N-acetylneuraminate transmembrane transporter activity TAS enables MF false ACCEPT "Reactome (""Defective SLC35A1 does not exchange CMP-Neu5Ac for CMP"") asserts the core CMP-sialic acid transporter activity in the disease context. Consistent with the core function." reaction_mf_exact accepted_mf reactome_disease_event 1 human SLC35A1 genes/human/SLC35A1/SLC35A1-ai-review.yaml SLC35A1 P78382 NCBITaxon:9606 Homo sapiens 22 R-HSA-727807 SLC35A1 exchanges CMP-Neu5Ac for CMP yes reactome/R-HSA-727807.md SLC35A1 exchanges CMP-Neu5Ac for CMP Homo sapiens GO:0005456 CMP-N-acetylneuraminate transmembrane transporter activity TAS enables MF false ACCEPT "Reactome (""SLC35A1 exchanges CMP-Neu5Ac for CMP"") asserts the core CMP-sialic acid transporter activity. Consistent with the core function." reaction_mf_exact accepted_mf 1 human SLC35B2 genes/human/SLC35B2/SLC35B2-ai-review.yaml SLC35B2 Q8TB61 NCBITaxon:9606 Homo sapiens 16 R-HSA-741449 SLC35B2,3 exchange cytosolic PAPS with Golgi PAP yes reactome/R-HSA-741449.md SLC35B2,3 exchange cytosolic PAPS with Golgi PAP Homo sapiens GO:0046964 3'-phosphoadenosine 5'-phosphosulfate transmembrane transporter activity TAS enables MF false ACCEPT Correct core molecular function, curated by Reactome, matching the experimentally demonstrated PAPS transporter activity. reaction_mf_exact accepted_mf multi_gene_reactome_id 2 human SLC35B3 genes/human/SLC35B3/SLC35B3-ai-review.yaml SLC35B3 Q9H1N7 NCBITaxon:9606 Homo sapiens 12 R-HSA-741449 SLC35B2,3 exchange cytosolic PAPS with Golgi PAP yes reactome/R-HSA-741449.md SLC35B2,3 exchange cytosolic PAPS with Golgi PAP Homo sapiens GO:0046964 3'-phosphoadenosine 5'-phosphosulfate transmembrane transporter activity TAS enables MF false ACCEPT Reactome author statement of PAPS transmembrane transporter activity, concordant with the experimental IDA annotation. Correct core molecular function. reaction_mf_exact accepted_mf multi_gene_reactome_id 2 human SLC37A4 genes/human/SLC37A4/SLC37A4-ai-review.yaml SLC37A4 O43826 NCBITaxon:9606 Homo sapiens 12 R-HSA-3229118 Defective SLC37A4 does not exchange G6P and Pi across the ER membrane no GO:0061513 glucose 6-phosphate:phosphate antiporter activity TAS enables MF false ACCEPT TAS (Reactome) annotation for the G6P:Pi antiporter activity across the ER membrane. Consistent with the experimental evidence. reaction_mf_exact accepted_mf reactome_disease_event|uncached_reactome 1 human SLC52A1 genes/human/SLC52A1/SLC52A1-ai-review.yaml SLC52A1 Q9NWF4 NCBITaxon:9606 Homo sapiens 15 R-HSA-3165230 SLC52A1,2,3 transport RIB from extracellular region to cytosol yes reactome/R-HSA-3165230.md SLC52A1,2,3 transport RIB from extracellular region to cytosol Homo sapiens GO:0032217 riboflavin transmembrane transporter activity TAS enables MF false ACCEPT TAS (Reactome) assertion of the core riboflavin transmembrane transporter activity, describing SLC52A1/2/3 transport of riboflavin from the extracellular region to the cytosol. Correct core function; retain. reaction_mf_exact accepted_mf multi_gene_reactome_id 3 human SLC52A2 genes/human/SLC52A2/SLC52A2-ai-review.yaml SLC52A2 Q9HAB3 NCBITaxon:9606 Homo sapiens 13 R-HSA-3165230 SLC52A1,2,3 transport RIB from extracellular region to cytosol yes reactome/R-HSA-3165230.md SLC52A1,2,3 transport RIB from extracellular region to cytosol Homo sapiens GO:0032217 riboflavin transmembrane transporter activity TAS enables MF false ACCEPT "Reactome TAS for the reaction ""SLC52A1,2,3 transport RIB from extracellular region to cytosol"". Correctly captures the core riboflavin transmembrane transporter activity of SLC52A2." reaction_mf_exact accepted_mf multi_gene_reactome_id 3 human SLC52A3 genes/human/SLC52A3/SLC52A3-ai-review.yaml SLC52A3 Q9NQ40 NCBITaxon:9606 Homo sapiens 28 R-HSA-3165230 SLC52A1,2,3 transport RIB from extracellular region to cytosol yes reactome/R-HSA-3165230.md SLC52A1,2,3 transport RIB from extracellular region to cytosol Homo sapiens GO:0032217 riboflavin transmembrane transporter activity TAS enables MF false ACCEPT "Reactome (TAS) statement of RFVT3 riboflavin transporter activity (reaction ""SLC52A1,2,3 transport RIB from extracellular region to cytosol""). Correct core molecular function." reaction_mf_exact accepted_mf multi_gene_reactome_id 3 human SLC6A8 genes/human/SLC6A8/SLC6A8-ai-review.yaml SLC6A8 P48029 NCBITaxon:9606 Homo sapiens 21 R-HSA-200396 Creatine transport across the plasma membrane yes reactome/R-HSA-200396.md Creatine transport across the plasma membrane Homo sapiens GO:0005309 creatine:sodium symporter activity TAS enables MF false ACCEPT Core molecular function captured via Reactome pathway curation. Correct. reaction_mf_exact accepted_mf local_module_mf 1 creatine_phosphocreatine_system:GO:0005309 creatine:sodium symporter activity human SLC8B1 genes/human/SLC8B1/SLC8B1-ai-review.yaml SLC8B1 Q6J4K2 NCBITaxon:9606 Homo sapiens 28 R-HSA-425822 K+-independent Li+/Ca2+ exchanger transport yes reactome/R-HSA-425822.md K+-independent Li+/Ca2+ exchanger transport Homo sapiens GO:0015368 calcium:monoatomic cation antiporter activity TAS enables MF false ACCEPT Calcium exchange against an unspecified monovalent cation. reaction_mf_exact accepted_mf 1 human SMOX genes/human/SMOX/SMOX-ai-review.yaml SMOX Q9NWM0 NCBITaxon:9606 Homo sapiens 14 R-HSA-141341 SMOX-3 oxidises SPN to SPM yes reactome/R-HSA-141341.md SMOX-3 oxidises SPN to SPM Homo sapiens GO:0052901 spermine oxidase activity TAS enables MF false ACCEPT TAS annotation from the Reactome reaction SMOX-3 oxidises SPN to SPM (spermine oxidase flavoenzyme catalysing oxidation of spermine to spermidine). Correct specific molecular function, corroborating the experimental spermine oxidase activity annotations. reaction_mf_exact accepted_mf 1 human SMPD1 genes/human/SMPD1/SMPD1-ai-review.yaml SMPD1 P17405 NCBITaxon:9606 Homo sapiens 25 R-HSA-1605797 SMPD1 hydrolyzes SPHM yes reactome/R-HSA-1605797.md SMPD1 hydrolyzes SPHM Homo sapiens GO:0004767 sphingomyelin phosphodiesterase activity TAS enables MF false ACCEPT "Reactome reaction ""SMPD1 hydrolyzes SPHM"" asserts the core sphingomyelin phosphodiesterase activity. Correct and consistent with all other evidence." reaction_mf_exact accepted_mf 1 human SMPD1 genes/human/SMPD1/SMPD1-ai-review.yaml SMPD1 P17405 NCBITaxon:9606 Homo sapiens 26 R-HSA-9769742 SMPD1 converts sphingomyelin to ceramide yes reactome/R-HSA-9769742.md SMPD1 converts sphingomyelin to ceramide Homo sapiens GO:0004767 sphingomyelin phosphodiesterase activity TAS enables MF false ACCEPT "Reactome reaction ""SMPD1 converts sphingomyelin to ceramide"" asserts the core MF. Duplicate of the other Reactome/experimental MF annotations." reaction_mf_exact accepted_mf local_module_mf 1 glycosphingolipid_lysosomal_degradation:GO:0004767 sphingomyelin phosphodiesterase activity human SOS1 genes/human/SOS1/SOS1-ai-review.yaml SOS1 Q07889 NCBITaxon:9606 Homo sapiens 10 R-HSA-177938 SOS1-mediated nucleotide exchange of RAS (EGF:EGFR:GRB2:SOS1) yes reactome/R-HSA-177938.md SOS1-mediated nucleotide exchange of RAS (EGF:EGFR:GRB2:SOS1) Homo sapiens GO:0005085 guanyl-nucleotide exchange factor activity TAS enables MF false ACCEPT SOS1 is a guanyl-nucleotide exchange factor that catalyzes GDP release from RAS (H-, N-, K-RAS), the core molecular function of the protein. reaction_mf_exact accepted_mf 1 human SOS1 genes/human/SOS1/SOS1-ai-review.yaml SOS1 Q07889 NCBITaxon:9606 Homo sapiens 11 R-HSA-177945 SOS1-mediated nucleotide exchange of RAS (EGF:EGFR:SHC1:GRB2:SOS1) yes reactome/R-HSA-177945.md SOS1-mediated nucleotide exchange of RAS (EGF:EGFR:SHC1:GRB2:SOS1) Homo sapiens GO:0005085 guanyl-nucleotide exchange factor activity TAS enables MF false ACCEPT SOS1 is a guanyl-nucleotide exchange factor that catalyzes GDP release from RAS (H-, N-, K-RAS), the core molecular function of the protein. reaction_mf_exact accepted_mf 1 human SOS1 genes/human/SOS1/SOS1-ai-review.yaml SOS1 Q07889 NCBITaxon:9606 Homo sapiens 12 R-HSA-186834 SOS-mediated nucleotide exchange on RAS (PDGF receptor:GRB2:SOS) yes reactome/R-HSA-186834.md SOS-mediated nucleotide exchange on RAS (PDGF receptor:GRB2:SOS) Homo sapiens GO:0005085 guanyl-nucleotide exchange factor activity TAS enables MF false ACCEPT SOS1 is a guanyl-nucleotide exchange factor that catalyzes GDP release from RAS (H-, N-, K-RAS), the core molecular function of the protein. reaction_mf_exact accepted_mf 1 human SOS1 genes/human/SOS1/SOS1-ai-review.yaml SOS1 Q07889 NCBITaxon:9606 Homo sapiens 13 R-HSA-210977 Sos-mediated nucleotide exchange of Ras (Tie2 receptor:Grb2:Sos) yes reactome/R-HSA-210977.md Sos-mediated nucleotide exchange of Ras (Tie2 receptor:Grb2:Sos) Homo sapiens GO:0005085 guanyl-nucleotide exchange factor activity TAS enables MF false ACCEPT SOS1 is a guanyl-nucleotide exchange factor that catalyzes GDP release from RAS (H-, N-, K-RAS), the core molecular function of the protein. reaction_mf_exact accepted_mf 1 human SOS1 genes/human/SOS1/SOS1-ai-review.yaml SOS1 Q07889 NCBITaxon:9606 Homo sapiens 14 R-HSA-2424477 SOS mediated nucleotide exchange of RAS (SHC) yes reactome/R-HSA-2424477.md SOS mediated nucleotide exchange of RAS (SHC) Homo sapiens GO:0005085 guanyl-nucleotide exchange factor activity TAS enables MF false ACCEPT SOS1 is a guanyl-nucleotide exchange factor that catalyzes GDP release from RAS (H-, N-, K-RAS), the core molecular function of the protein. reaction_mf_exact accepted_mf 1 human SOS1 genes/human/SOS1/SOS1-ai-review.yaml SOS1 Q07889 NCBITaxon:9606 Homo sapiens 15 R-HSA-392054 NCAM1:pFAK:Grb2:Sos-mediated nucleotide exchange of Ras yes reactome/R-HSA-392054.md NCAM1:pFAK:Grb2:Sos-mediated nucleotide exchange of Ras Homo sapiens GO:0005085 guanyl-nucleotide exchange factor activity TAS enables MF false ACCEPT SOS1 is a guanyl-nucleotide exchange factor that catalyzes GDP release from RAS (H-, N-, K-RAS), the core molecular function of the protein. reaction_mf_exact accepted_mf 1 human SOS1 genes/human/SOS1/SOS1-ai-review.yaml SOS1 Q07889 NCBITaxon:9606 Homo sapiens 16 R-HSA-9607304 SOS1-mediated nucleotide exchange of RAS downstream of FLT3 yes reactome/R-HSA-9607304.md SOS1-mediated nucleotide exchange of RAS downstream of FLT3 Homo sapiens GO:0005085 guanyl-nucleotide exchange factor activity TAS enables MF false ACCEPT SOS1 is a guanyl-nucleotide exchange factor that catalyzes GDP release from RAS (H-, N-, K-RAS), the core molecular function of the protein. reaction_mf_exact accepted_mf 1 human SOS1 genes/human/SOS1/SOS1-ai-review.yaml SOS1 Q07889 NCBITaxon:9606 Homo sapiens 17 R-HSA-9670436 p-KIT mutants:GRB2:SOS catalyzes nucleotide exchange on RAS yes reactome/R-HSA-9670436.md p-KIT mutants:GRB2:SOS catalyzes nucleotide exchange on RAS Homo sapiens GO:0005085 guanyl-nucleotide exchange factor activity TAS enables MF false ACCEPT SOS1 is a guanyl-nucleotide exchange factor that catalyzes GDP release from RAS (H-, N-, K-RAS), the core molecular function of the protein. reaction_mf_exact accepted_mf 1 human SOS1 genes/human/SOS1/SOS1-ai-review.yaml SOS1 Q07889 NCBITaxon:9606 Homo sapiens 18 R-HSA-9672170 SOS-mediated nucleotide exchange of RAS downstream of mutant PDGFR receptors yes reactome/R-HSA-9672170.md SOS-mediated nucleotide exchange of RAS downstream of mutant PDGFR receptors Homo sapiens GO:0005085 guanyl-nucleotide exchange factor activity TAS enables MF false ACCEPT SOS1 is a guanyl-nucleotide exchange factor that catalyzes GDP release from RAS (H-, N-, K-RAS), the core molecular function of the protein. reaction_mf_exact accepted_mf 1 human SOS1 genes/human/SOS1/SOS1-ai-review.yaml SOS1 Q07889 NCBITaxon:9606 Homo sapiens 19 R-HSA-9695853 FLT3 mutants:GRB2:SOS1-mediated nucleotide exchange on RAS yes reactome/R-HSA-9695853.md FLT3 mutants:GRB2:SOS1-mediated nucleotide exchange on RAS Homo sapiens GO:0005085 guanyl-nucleotide exchange factor activity TAS enables MF false ACCEPT SOS1 is a guanyl-nucleotide exchange factor that catalyzes GDP release from RAS (H-, N-, K-RAS), the core molecular function of the protein. reaction_mf_exact accepted_mf 1 human SOS1 genes/human/SOS1/SOS1-ai-review.yaml SOS1 Q07889 NCBITaxon:9606 Homo sapiens 20 R-HSA-9703441 SOS1-mediated nucleotide exchange of RAS downstream of FLT3 fusion mutants yes reactome/R-HSA-9703441.md SOS1-mediated nucleotide exchange of RAS downstream of FLT3 fusion mutants Homo sapiens GO:0005085 guanyl-nucleotide exchange factor activity TAS enables MF false ACCEPT SOS1 is a guanyl-nucleotide exchange factor that catalyzes GDP release from RAS (H-, N-, K-RAS), the core molecular function of the protein. reaction_mf_exact accepted_mf 1 human SPHK1 genes/human/SPHK1/SPHK1-ai-review.yaml SPHK1 Q9NYA1 NCBITaxon:9606 Homo sapiens 39 R-HSA-5218845 p-SPHK1 phosphorylates sphingosine to sphingosine 1-phosphate yes reactome/R-HSA-5218845.md p-SPHK1 phosphorylates sphingosine to sphingosine 1-phosphate Homo sapiens GO:0008481 sphingosine kinase activity TAS enables MF false ACCEPT Reactome TAS of the core sphingosine kinase activity. reaction_mf_exact accepted_mf 1 human SPHK1 genes/human/SPHK1/SPHK1-ai-review.yaml SPHK1 Q9NYA1 NCBITaxon:9606 Homo sapiens 40 R-HSA-9625814 SPHK1 phosphorylates sphingosine in response to E2 stimulation yes reactome/R-HSA-9625814.md SPHK1 phosphorylates sphingosine in response to E2 stimulation Homo sapiens GO:0008481 sphingosine kinase activity TAS enables MF false ACCEPT Reactome TAS of the core sphingosine kinase activity (estrogen-stimulated context). reaction_mf_exact accepted_mf 1 human SQSTM1 genes/human/SQSTM1/SQSTM1-ai-review.yaml SQSTM1 Q13501 NCBITaxon:9606 Homo sapiens 195 R-HSA-205008 Polyubiquitinated NRIF binds to p62 (Sequestosome) yes reactome/R-HSA-205008.md Polyubiquitinated NRIF binds to p62 (Sequestosome) Homo sapiens GO:0043130 ubiquitin binding TAS enables MF false ACCEPT Direct evidence that p62 binds ubiquitin via its UBA domain. reaction_mf_exact accepted_mf 1 human SRD5A2 genes/human/SRD5A2/SRD5A2-ai-review.yaml SRD5A2 P31213 NCBITaxon:9606 Homo sapiens 36 R-HSA-9705713 SRD5A2 dehydrogenates TEST to DHTEST yes reactome/R-HSA-9705713.md SRD5A2 dehydrogenates TEST to DHTEST Homo sapiens GO:0003865 3-oxo-5-alpha-steroid 4-dehydrogenase activity TAS enables MF false ACCEPT Reactome TAS for the core enzymatic activity, based on the SRD5A2-catalyzed testosterone-to-DHT reaction. Matches the experimental and IBA molecular function. reaction_mf_exact accepted_mf local_module_mf 1 sex_steroid_biosynthesis:GO:0003865 3-oxo-5-alpha-steroid 4-dehydrogenase activity human ST6GAL1 genes/human/ST6GAL1/ST6GAL1-ai-review.yaml ST6GAL1 P15907 NCBITaxon:9606 Homo sapiens 12 R-HSA-4085033 ST6GAL1,2 transfer Neu5Ac to terminal Gal (alpha-2,6 link) yes reactome/R-HSA-4085033.md ST6GAL1,2 transfer Neu5Ac to terminal Gal (alpha-2,6 link) Homo sapiens GO:0003835 beta-galactoside alpha-2,6-sialyltransferase activity TAS enables MF false ACCEPT "Core catalytic MF (Reactome reaction ""ST6GAL1,2 transfer Neu5Ac to terminal Gal (alpha-2,6 link)""). Correct; redundant with the experimentally supported annotations to the same term." reaction_mf_exact accepted_mf 1 human ST6GAL1 genes/human/ST6GAL1/ST6GAL1-ai-review.yaml ST6GAL1 P15907 NCBITaxon:9606 Homo sapiens 13 R-HSA-975902 ST6GAL1 transfers Neu5Ac to terminal Gal of N-glycans yes reactome/R-HSA-975902.md ST6GAL1 transfers Neu5Ac to terminal Gal of N-glycans Homo sapiens GO:0003835 beta-galactoside alpha-2,6-sialyltransferase activity TAS enables MF false ACCEPT "Core catalytic MF (Reactome ""ST6GAL1 transfers Neu5Ac to terminal Gal of N-glycans""). Correct; redundant duplicate." reaction_mf_exact accepted_mf 1 human ST6GAL1 genes/human/ST6GAL1/ST6GAL1-ai-review.yaml ST6GAL1 P15907 NCBITaxon:9606 Homo sapiens 14 R-HSA-977071 ST6GAL1 transfers sialic acid to Tn antigens to form sTn antigens yes reactome/R-HSA-977071.md ST6GAL1 transfers sialic acid to Tn antigens to form sTn antigens Homo sapiens GO:0003835 beta-galactoside alpha-2,6-sialyltransferase activity TAS enables MF false ACCEPT Core catalytic MF; this Reactome reaction concerns transfer of sialic acid to Tn antigens to form sialyl-Tn. The activity is correct (same enzyme), though the O-GalNAc/sTn acceptor is a minor context relative to the N-glycan role. reaction_mf_exact accepted_mf 1 human ST8SIA1 genes/human/ST8SIA1/ST8SIA1-ai-review.yaml ST8SIA1 Q92185 NCBITaxon:9606 Homo sapiens 10 R-HSA-4084978 ST8SIA1-6 transfer Neu5Ac to terminal Neu5Ac residues yes reactome/R-HSA-4084978.md ST8SIA1-6 transfer Neu5Ac to terminal Neu5Ac residues Homo sapiens GO:0003828 alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity TAS enables MF false ACCEPT Reactome (TAS) assertion of the core alpha-2,8-sialyltransferase activity, matching the experimental evidence. Core function. reaction_mf_exact accepted_mf 1 human STT3A genes/human/STT3A/STT3A-ai-review.yaml STT3A P46977 NCBITaxon:9606 Homo sapiens 18 R-HSA-9816276 CDH1 is N-glycosylated on asparagine residues in endoplasmic reticulum yes reactome/R-HSA-9816276.md CDH1 is N-glycosylated on asparagine residues in endoplasmic reticulum Homo sapiens GO:0004579 dolichyl-diphosphooligosaccharide-protein glycotransferase activity TAS enables MF false ACCEPT Reactome-curated (TAS) assignment of the core catalytic activity, in the context of CDH1 (E-cadherin) N-glycosylation in the ER. This is STT3A's own correct core molecular function. reaction_mf_exact accepted_mf 1 human STUB1 genes/human/STUB1/STUB1-ai-review.yaml STUB1 Q9UNE7 NCBITaxon:9606 Homo sapiens 76 R-HSA-6807134 Reactome pathway R-HSA-6807134 yes reactome/R-HSA-6807134.md NEDD4, WWP2, CHIP and XIAP polyubiquitinate PTEN Homo sapiens GO:0061630 ubiquitin protein ligase activity TAS enables MF false ACCEPT STUB1/CHIP is a U-box-type E3 ubiquitin-protein ligase; ubiquitin protein ligase activity is a core molecular function. reaction_mf_exact accepted_mf 1 human STUB1 genes/human/STUB1/STUB1-ai-review.yaml STUB1 Q9UNE7 NCBITaxon:9606 Homo sapiens 77 R-HSA-9009308 Reactome pathway R-HSA-9009308 no GO:0061630 ubiquitin protein ligase activity TAS enables MF false ACCEPT STUB1/CHIP is a U-box-type E3 ubiquitin-protein ligase; ubiquitin protein ligase activity is a core molecular function. reaction_mf_exact accepted_mf uncached_reactome 1 human STUB1 genes/human/STUB1/STUB1-ai-review.yaml STUB1 Q9UNE7 NCBITaxon:9606 Homo sapiens 92 R-HSA-1918092 Reactome pathway R-HSA-1918092 no GO:0004842 ubiquitin-protein transferase activity TAS enables MF false ACCEPT STUB1/CHIP is a U-box-type E3 ubiquitin-protein ligase; ubiquitin-protein transferase activity is a core molecular function. reaction_mf_exact accepted_mf uncached_reactome 1 human STUB1 genes/human/STUB1/STUB1-ai-review.yaml STUB1 Q9UNE7 NCBITaxon:9606 Homo sapiens 93 R-HSA-2187368 Reactome pathway R-HSA-2187368 yes reactome/R-HSA-2187368.md STUB1 (CHIP) ubiquitinates SMAD3 Homo sapiens GO:0004842 ubiquitin-protein transferase activity TAS enables MF false ACCEPT STUB1/CHIP is a U-box-type E3 ubiquitin-protein ligase; ubiquitin-protein transferase activity is a core molecular function. reaction_mf_exact accepted_mf 1 human SUMF2 genes/human/SUMF2/SUMF2-ai-review.yaml SUMF2 Q8NBJ7 NCBITaxon:9606 Homo sapiens 12 R-HSA-1614336 Sulfatase activation by SUMF1 (FGE) no GO:0004857 enzyme inhibitor activity TAS enables MF false ACCEPT Enzyme inhibitor activity: SUMF2 inhibits the sulfatase-activating (formylglycine-generating) activity of SUMF1 via heterodimerization. This is SUMF2's defining molecular role. Falcon deep research reinforces that SUMF2/pFGE is catalytically inactive yet binds sulfatase peptides and acts as a negative regulator of sulfatase activation, and that co-expression of pFGE compromises FGE's ability to generate active sulfatases. reaction_mf_exact accepted_mf uncached_reactome 1 human SYVN1 genes/human/SYVN1/SYVN1-ai-review.yaml SYVN1 Q86TM6 NCBITaxon:9606 Homo sapiens 28 R-HSA-8867288 OS9:SEL1:ERAD E3 ligase:DERL2 ubiquitinates unfolded protein:(GlcNAc)2 (Man)9-5 yes reactome/R-HSA-8867288.md OS9:SEL1:ERAD E3 ligase:DERL2 ubiquitinates unfolded protein:(GlcNAc)2 (Man)9-5 Homo sapiens GO:0061630 ubiquitin protein ligase activity TAS enables MF false ACCEPT TAS from Reactome pathway for ubiquitination of unfolded glycoproteins by the OS9:SEL1:ERAD E3 ligase:DERL2 complex. Consistent with core function. reaction_mf_exact accepted_mf multi_gene_reactome_id 4 human TBK1 genes/human/TBK1/TBK1-ai-review.yaml TBK1 Q9UHD2 NCBITaxon:9606 Homo sapiens 74 R-HSA-2396007 IRF3 is phosphorylated by TBK1 yes reactome/R-HSA-2396007.md IRF3 is phosphorylated by TBK1 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT TBK1 is a non-canonical IKK-related serine/threonine protein kinase; this is its core, extensively documented molecular function (trans-autophosphorylation at Ser172; phosphorylation of IRF3/IRF7, the adaptors MAVS/STING1/TICAM1, OPTN, SMCR8, ATG8s, etc.). reaction_mf_exact accepted_mf 1 human TBK1 genes/human/TBK1/TBK1-ai-review.yaml TBK1 Q9UHD2 NCBITaxon:9606 Homo sapiens 75 R-HSA-3249371 TBK1 phosphorylates STAT6 at Ser407 yes reactome/R-HSA-3249371.md TBK1 phosphorylates STAT6 at Ser407 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT TBK1 is a non-canonical IKK-related serine/threonine protein kinase; this is its core, extensively documented molecular function (trans-autophosphorylation at Ser172; phosphorylation of IRF3/IRF7, the adaptors MAVS/STING1/TICAM1, OPTN, SMCR8, ATG8s, etc.). reaction_mf_exact accepted_mf 1 human TBK1 genes/human/TBK1/TBK1-ai-review.yaml TBK1 Q9UHD2 NCBITaxon:9606 Homo sapiens 76 R-HSA-933525 Phosphorylation and release of IRF7 yes reactome/R-HSA-933525.md Phosphorylation and release of IRF7 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT TBK1 is a non-canonical IKK-related serine/threonine protein kinase; this is its core, extensively documented molecular function (trans-autophosphorylation at Ser172; phosphorylation of IRF3/IRF7, the adaptors MAVS/STING1/TICAM1, OPTN, SMCR8, ATG8s, etc.). reaction_mf_exact accepted_mf 1 human TNFAIP3 genes/human/TNFAIP3/TNFAIP3-ai-review.yaml TNFAIP3 P21580 NCBITaxon:9606 Homo sapiens 78 R-HSA-5357845 K63polyUb-RIPK1 is deubiquitinated yes reactome/R-HSA-5357845.md K63polyUb-RIPK1 is deubiquitinated Homo sapiens GO:0061578 K63-linked deubiquitinase activity TAS enables MF false ACCEPT "TAS from Reactome ""K63polyUb-RIPK1 is deubiquitinated"". A20 removes K63-linked polyubiquitin from RIPK1." reaction_mf_exact accepted_mf 1 human TNFAIP3 genes/human/TNFAIP3/TNFAIP3-ai-review.yaml TNFAIP3 P21580 NCBITaxon:9606 Homo sapiens 79 R-HSA-5690856 TNFAIP3 (A20) deubiquitinates K63polyUb-RIPK1 yes reactome/R-HSA-5690856.md TNFAIP3 (A20) deubiquitinates K63polyUb-RIPK1 Homo sapiens GO:0061578 K63-linked deubiquitinase activity TAS enables MF false ACCEPT "TAS from Reactome ""TNFAIP3 (A20) deubiquitinates K63polyUb-RIPK1""." reaction_mf_exact accepted_mf 1 human TNFAIP3 genes/human/TNFAIP3/TNFAIP3-ai-review.yaml TNFAIP3 P21580 NCBITaxon:9606 Homo sapiens 80 R-HSA-8869506 TNFAIP3 in OTUD7B:TNFAIP3:ZRANB1 deubiquitinates K63polyUb-TRAF6 yes reactome/R-HSA-8869506.md TNFAIP3 in OTUD7B:TNFAIP3:ZRANB1 deubiquitinates K63polyUb-TRAF6 Homo sapiens GO:0061578 K63-linked deubiquitinase activity TAS enables MF false ACCEPT "TAS from Reactome ""TNFAIP3 in OTUD7B:TNFAIP3:ZRANB1 deubiquitinates K63polyUb-TRAF6"". A20 also deubiquitinates K63-linked chains on TRAF6 as part of a complex." reaction_mf_exact accepted_mf 1 human TNKS genes/human/TNKS/TNKS-ai-review.yaml TNKS O95271 NCBITaxon:9606 Homo sapiens 55 R-HSA-3640858 Tankyrase ADP-ribosylates AXIN yes reactome/R-HSA-3640858.md Tankyrase ADP-ribosylates AXIN Homo sapiens GO:0003950 NAD+ poly-ADP-ribosyltransferase activity TAS enables MF false ACCEPT NAD+ poly-ADP-ribosyltransferase activity is a core TNKS catalytic molecular function. reaction_mf_exact accepted_mf 1 human TNKS genes/human/TNKS/TNKS-ai-review.yaml TNKS O95271 NCBITaxon:9606 Homo sapiens 56 R-HSA-8948800 TNKS and TNKS2 PARylate PTEN yes reactome/R-HSA-8948800.md TNKS and TNKS2 PARylate PTEN Homo sapiens GO:0003950 NAD+ poly-ADP-ribosyltransferase activity TAS enables MF false ACCEPT NAD+ poly-ADP-ribosyltransferase activity is a core TNKS catalytic molecular function. reaction_mf_exact accepted_mf 1 human TRAF6 genes/human/TRAF6/TRAF6-ai-review.yaml TRAF6 Q9Y4K3 NCBITaxon:9606 Homo sapiens 4 R-HSA-202453 TRAF6 pathway yes reactome/R-HSA-202453.md Auto-ubiquitination of TRAF6 Homo sapiens GO:0004842 ubiquitin-protein transferase activity TAS enables MF false ACCEPT Pathway database annotation based on traceable author statement. reaction_mf_exact accepted_mf 1 human TRAF6 genes/human/TRAF6/TRAF6-ai-review.yaml TRAF6 Q9Y4K3 NCBITaxon:9606 Homo sapiens 54 R-HSA-202534 Ubiquitination of NEMO by TRAF6 yes reactome/R-HSA-202534.md Ubiquitination of NEMO by TRAF6 Homo sapiens GO:0004842 ubiquitin-protein transferase activity TAS enables MF false ACCEPT Pathway database annotation for TRAF6 E3 ligase activity. reaction_mf_exact accepted_mf 1 human TRAF6 genes/human/TRAF6/TRAF6-ai-review.yaml TRAF6 Q9Y4K3 NCBITaxon:9606 Homo sapiens 55 R-HSA-2730904 Auto-ubiquitination of TRAF6 yes reactome/R-HSA-2730904.md Auto-ubiquitination of TRAF6 Homo sapiens GO:0004842 ubiquitin-protein transferase activity TAS enables MF false ACCEPT Pathway database annotation for TRAF6 E3 ligase activity. reaction_mf_exact accepted_mf 1 human TRAF6 genes/human/TRAF6/TRAF6-ai-review.yaml TRAF6 Q9Y4K3 NCBITaxon:9606 Homo sapiens 56 R-HSA-446877 TRAF6 is K63 poly-ubiquitinated yes reactome/R-HSA-446877.md TRAF6 is K63 poly-ubiquitinated Homo sapiens GO:0004842 ubiquitin-protein transferase activity TAS enables MF false ACCEPT Pathway database annotation for TRAF6 E3 ligase activity. reaction_mf_exact accepted_mf 1 human TRAF6 genes/human/TRAF6/TRAF6-ai-review.yaml TRAF6 Q9Y4K3 NCBITaxon:9606 Homo sapiens 57 R-HSA-450358 Activated TRAF6 synthesizes unanchored polyubiquitin chains upon TLR stimulation yes reactome/R-HSA-450358.md Activated TRAF6 synthesizes unanchored polyubiquitin chains upon TLR stimulation Homo sapiens GO:0004842 ubiquitin-protein transferase activity TAS enables MF false ACCEPT Pathway database annotation for TRAF6 E3 ligase activity. reaction_mf_exact accepted_mf 1 human TRAF6 genes/human/TRAF6/TRAF6-ai-review.yaml TRAF6 Q9Y4K3 NCBITaxon:9606 Homo sapiens 58 R-HSA-936942 Auto ubiquitination of oligo-TRAF6 bound to p-IRAK2 yes reactome/R-HSA-936942.md Auto ubiquitination of oligo-TRAF6 bound to p-IRAK2 Homo sapiens GO:0004842 ubiquitin-protein transferase activity TAS enables MF false ACCEPT Pathway database annotation for TRAF6 E3 ligase activity. reaction_mf_exact accepted_mf 1 human TRAF6 genes/human/TRAF6/TRAF6-ai-review.yaml TRAF6 Q9Y4K3 NCBITaxon:9606 Homo sapiens 59 R-HSA-936986 Activated TRAF6 synthesizes unanchored polyubiquitin chains yes reactome/R-HSA-936986.md Activated TRAF6 synthesizes unanchored polyubiquitin chains Homo sapiens GO:0004842 ubiquitin-protein transferase activity TAS enables MF false ACCEPT Pathway database annotation for TRAF6 E3 ligase activity. reaction_mf_exact accepted_mf 1 human TRAF6 genes/human/TRAF6/TRAF6-ai-review.yaml TRAF6 Q9Y4K3 NCBITaxon:9606 Homo sapiens 60 R-HSA-9645394 Activated TRAF6 synthesizes unanchored polyubiquitin chains upon ALPK1:ADP-heptose stimulation yes reactome/R-HSA-9645394.md Activated TRAF6 synthesizes unanchored polyubiquitin chains upon ALPK1:ADP-heptose stimulation Homo sapiens GO:0004842 ubiquitin-protein transferase activity TAS enables MF false ACCEPT Pathway database annotation for TRAF6 E3 ligase activity. reaction_mf_exact accepted_mf 1 human TRAF6 genes/human/TRAF6/TRAF6-ai-review.yaml TRAF6 Q9Y4K3 NCBITaxon:9606 Homo sapiens 61 R-HSA-9645414 Auto ubiquitination of TRAF6 bound to ALPK1:ADP-heptose:TIFA oligomer yes reactome/R-HSA-9645414.md Auto ubiquitination of TRAF6 bound to ALPK1:ADP-heptose:TIFA oligomer Homo sapiens GO:0004842 ubiquitin-protein transferase activity TAS enables MF false ACCEPT Pathway database annotation for TRAF6 E3 ligase activity. reaction_mf_exact accepted_mf 1 human TRAF6 genes/human/TRAF6/TRAF6-ai-review.yaml TRAF6 Q9Y4K3 NCBITaxon:9606 Homo sapiens 62 R-HSA-975147 Auto ubiquitination of oligo-TRAF6 bound to p-IRAK2 at endosome membrane yes reactome/R-HSA-975147.md Auto ubiquitination of oligo-TRAF6 bound to p-IRAK2 at endosome membrane Homo sapiens GO:0004842 ubiquitin-protein transferase activity TAS enables MF false ACCEPT Pathway database annotation for TRAF6 E3 ligase activity. reaction_mf_exact accepted_mf 1 human TRIM13 genes/human/TRIM13/TRIM13-ai-review.yaml TRIM13 O60858 NCBITaxon:9606 Homo sapiens 15 R-HSA-8867288 OS9:SEL1:ERAD E3 ligase:DERL2 ubiquitinates unfolded protein:(GlcNAc)2 (Man)9-5 yes reactome/R-HSA-8867288.md OS9:SEL1:ERAD E3 ligase:DERL2 ubiquitinates unfolded protein:(GlcNAc)2 (Man)9-5 Homo sapiens GO:0061630 ubiquitin protein ligase activity TAS enables MF false ACCEPT Reactome curation of TRIM13 ubiquitin ligase activity in the ERAD pathway. Core MF. reaction_mf_exact accepted_mf multi_gene_reactome_id 4 human UCHL1 genes/human/UCHL1/UCHL1-ai-review.yaml UCHL1 P09936 NCBITaxon:9606 Homo sapiens 18 R-HSA-5690319 UCHL1, UCHL3 cleave ubiquitin adducts yes reactome/R-HSA-5690319.md UCHL1, UCHL3 cleave ubiquitin adducts Homo sapiens GO:0004843 cysteine-type deubiquitinase activity TAS enables MF false ACCEPT UCHL1 directly enables cysteine-type deubiquitinase / ubiquitin C-terminal hydrolase activity. reaction_mf_exact accepted_mf 1 human UCKL1 genes/human/UCKL1/UCKL1-ai-review.yaml UCKL1 Q9NWZ5 NCBITaxon:9606 Homo sapiens 20 R-HSA-8954327 UCKL1 phosphorylates urindine, cytidine yes reactome/R-HSA-8954327.md UCKL1 phosphorylates urindine, cytidine Homo sapiens GO:0004849 uridine kinase activity TAS enables MF false ACCEPT Reactome's uridine-phosphorylation event predates direct UCKL1 enzymology but is now confirmed by the 2022 biochemical study. reaction_mf_exact accepted_mf 1 human UGCG genes/human/UGCG/UGCG-ai-review.yaml UGCG Q16739 NCBITaxon:9606 Homo sapiens 19 R-HSA-1638104 UGCG transfers glucose to ceramide yes reactome/R-HSA-1638104.md UGCG transfers glucose to ceramide Homo sapiens GO:0008120 ceramide glucosyltransferase activity TAS enables MF false ACCEPT "TAS (Reactome) of the core molecular function. Matches the experimental IDA (PMID:8643456), UniProt EC 2.4.1.80 and the Reactome reaction ""UGCG transfers glucose to ceramide"". Accept." reaction_mf_exact accepted_mf 1 human UGGT1 genes/human/UGGT1/UGGT1-ai-review.yaml UGGT1 Q9NYU2 NCBITaxon:9606 Homo sapiens 16 R-HSA-548884 UGGT1,2 transfers glucose from DbGP to (un)folded protein:(GlcNAc)2 (Man)8b yes reactome/R-HSA-548884.md UGGT1,2 transfers glucose from DbGP to (un)folded protein:(GlcNAc)2 (Man)8b Homo sapiens GO:0003980 UDP-glucose:glycoprotein glucosyltransferase activity TAS enables MF false ACCEPT "TAS annotation from Reactome reaction R-HSA-548884 which models UGGT1/2 transferring glucose from dolichyl beta-D-glucosyl phosphate to unfolded protein glycans. The Reactome entry states that ""UGGT1 and 2 are able to distinguish proteins with minor folding defects in the ERQC and reglucosylate them."" This correctly captures the core enzymatic function of UGGT1." reaction_mf_exact accepted_mf 1 human UGT8 genes/human/UGT8/UGT8-ai-review.yaml UGT8 Q16880 NCBITaxon:9606 Homo sapiens 17 R-HSA-6785933 UGT8 transfers Gal from UDP-Gal to CERA yes reactome/R-HSA-6785933.md UGT8 transfers Gal from UDP-Gal to CERA Homo sapiens GO:0003851 N-acylsphingosine galactosyltransferase activity TAS enables MF false ACCEPT Reactome reaction (UGT8 transfers Gal from UDP-Gal to CERA) capturing the core catalytic activity. Consistent with all other MF annotations. Correct. reaction_mf_exact accepted_mf 1 human ULK1 genes/human/ULK1/ULK1-ai-review.yaml ULK1 O75385 NCBITaxon:9606 Homo sapiens 51 R-HSA-5675868 ULK1 phosphorylates ATG13 and RB1CC1 yes reactome/R-HSA-5675868.md ULK1 phosphorylates ATG13 and RB1CC1 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Protein serine/threonine kinase activity. ULK1's defining activity, supported by an EXP and eight independent IDA experiments. reaction_mf_exact accepted_mf 1 human ULK1 genes/human/ULK1/ULK1-ai-review.yaml ULK1 O75385 NCBITaxon:9606 Homo sapiens 52 R-HSA-8876446 p-ULK1 phosphorylates DENND3 yes reactome/R-HSA-8876446.md p-ULK1 phosphorylates DENND3 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Protein serine/threonine kinase activity. ULK1's defining activity, supported by an EXP and eight independent IDA experiments. reaction_mf_exact accepted_mf 1 human ULK1 genes/human/ULK1/ULK1-ai-review.yaml ULK1 O75385 NCBITaxon:9606 Homo sapiens 53 R-HSA-8948146 FUNDC1 is phosphorylated by ULK1 at Ser17 yes reactome/R-HSA-8948146.md FUNDC1 is phosphorylated by ULK1 at Ser17 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Protein serine/threonine kinase activity. ULK1's defining activity, supported by an EXP and eight independent IDA experiments. reaction_mf_exact accepted_mf 1 human UROS genes/human/UROS/UROS-ai-review.yaml UROS P10746 NCBITaxon:9606 Homo sapiens 15 R-HSA-189488 UROS transforms HMB to URO3 yes reactome/R-HSA-189488.md UROS transforms HMB to URO3 Homo sapiens GO:0004852 uroporphyrinogen-III synthase activity TAS enables MF false ACCEPT Reactome-curated statement of the core catalytic activity (HMB to uroporphyrinogen III). reaction_mf_exact accepted_mf 1 human USP10 genes/human/USP10/USP10-ai-review.yaml USP10 Q14694 NCBITaxon:9606 Homo sapiens 36 R-HSA-5653770 USP10 deubiquitinates monoUb:K164,ISG:K164,ISG:K168-PCNA yes reactome/R-HSA-5653770.md USP10 deubiquitinates monoUb:K164,ISG:K164,ISG:K168-PCNA Homo sapiens GO:0004843 cysteine-type deubiquitinase activity TAS enables MF false ACCEPT USP10 is a ubiquitin-specific cysteine deubiquitinase; this MF captures the core catalytic activity even though individual papers test different substrates. reaction_mf_exact accepted_mf 1 human USP10 genes/human/USP10/USP10-ai-review.yaml USP10 Q14694 NCBITaxon:9606 Homo sapiens 37 R-HSA-5689973 USP10,USP24,USP42 deubiquitinate TP53 yes reactome/R-HSA-5689973.md USP10,USP24,USP42 deubiquitinate TP53 Homo sapiens GO:0004843 cysteine-type deubiquitinase activity TAS enables MF false ACCEPT USP10 is a ubiquitin-specific cysteine deubiquitinase; this MF captures the core catalytic activity even though individual papers test different substrates. reaction_mf_exact accepted_mf 1 human USP10 genes/human/USP10/USP10-ai-review.yaml USP10 Q14694 NCBITaxon:9606 Homo sapiens 38 R-HSA-6782106 USP10 deubiquitinates SNX3, CFTR yes reactome/R-HSA-6782106.md USP10 deubiquitinates SNX3, CFTR Homo sapiens GO:0004843 cysteine-type deubiquitinase activity TAS enables MF false ACCEPT USP10 is a ubiquitin-specific cysteine deubiquitinase; this MF captures the core catalytic activity even though individual papers test different substrates. reaction_mf_exact accepted_mf 1 human USP21 genes/human/USP21/USP21-ai-review.yaml USP21 Q9UK80 NCBITaxon:9606 Homo sapiens 13 R-HSA-5690157 Ub-specific processing proteases (USP21 deubiquitinase activity) no GO:0004843 cysteine-type deubiquitinase activity TAS enables MF false ACCEPT Reactome (TAS) annotation of the core DUB activity. Correct and experimentally corroborated. reaction_mf_exact accepted_mf uncached_reactome 1 human USP21 genes/human/USP21/USP21-ai-review.yaml USP21 Q9UK80 NCBITaxon:9606 Homo sapiens 14 R-HSA-5690159 Ub-specific processing proteases (USP21 deubiquitinase activity) no GO:0004843 cysteine-type deubiquitinase activity TAS enables MF false ACCEPT Reactome (TAS) annotation of the core cysteine-type deubiquitinase activity. reaction_mf_exact accepted_mf uncached_reactome 1 human USP21 genes/human/USP21/USP21-ai-review.yaml USP21 Q9UK80 NCBITaxon:9606 Homo sapiens 15 R-HSA-6783177 Ub-specific processing proteases (USP21 deubiquitinase activity) yes reactome/R-HSA-6783177.md USP21 deubiquitinates GATA3,IL33 Homo sapiens GO:0004843 cysteine-type deubiquitinase activity TAS enables MF false ACCEPT Reactome (TAS) annotation of the core cysteine-type deubiquitinase activity. reaction_mf_exact accepted_mf 1 human USP25 genes/human/USP25/USP25-ai-review.yaml USP25 Q9UHP3 NCBITaxon:9606 Homo sapiens 27 R-HSA-5696564 Ub-specific processing proteases no GO:0004843 cysteine-type deubiquitinase activity TAS enables MF false ACCEPT Reactome curated annotation of cysteine-type deubiquitinase activity, the core catalytic molecular function of USP25. reaction_mf_exact accepted_mf uncached_reactome 1 human USP8 genes/human/USP8/USP8-ai-review.yaml USP8 P40818 NCBITaxon:9606 Homo sapiens 35 R-HSA-4641236 USP8 deubiquitinates FZD to potentiate WNT signaling yes reactome/R-HSA-4641236.md USP8 deubiquitinates FZD to potentiate WNT signaling Homo sapiens GO:0004843 cysteine-type deubiquitinase activity TAS enables MF false ACCEPT USP8 is a bona fide ubiquitin-specific cysteine deubiquitinase, including activity on K48- and K63-linked ubiquitin chains where tested. reaction_mf_exact accepted_mf 1 human USP8 genes/human/USP8/USP8-ai-review.yaml USP8 P40818 NCBITaxon:9606 Homo sapiens 36 R-HSA-8875443 USP8 deubiquitinates LRIG1 yes reactome/R-HSA-8875443.md USP8 deubiquitinates LRIG1 Homo sapiens GO:0004843 cysteine-type deubiquitinase activity TAS enables MF false ACCEPT USP8 is a bona fide ubiquitin-specific cysteine deubiquitinase, including activity on K48- and K63-linked ubiquitin chains where tested. reaction_mf_exact accepted_mf 1 human VPS4A genes/human/VPS4A/VPS4A-ai-review.yaml VPS4A Q9UN37 NCBITaxon:9606 Homo sapiens 66 R-HSA-9668415 VPS4 mediates disassembly of ESCRTIII subunits to promote sealing of holes in the nuclear envelope yes reactome/R-HSA-9668415.md VPS4 mediates disassembly of ESCRTIII subunits to promote sealing of holes in the nuclear envelope Homo sapiens GO:0016887 ATP hydrolysis activity TAS enables MF false ACCEPT Core VPS4A function: ATP-driven recognition, remodeling, and disassembly of ESCRT-III/protein assemblies. reaction_mf_exact accepted_mf 1 human WEE1 genes/human/WEE1/WEE1-ai-review.yaml WEE1 P30291 NCBITaxon:9606 Homo sapiens 11 R-HSA-170070 Wee1-mediated phosphorylation of Cyclin B1:phospho-Cdc2 complexes yes reactome/R-HSA-170070.md Wee1-mediated phosphorylation of Cyclin B1:phospho-Cdc2 complexes Homo sapiens GO:0004713 protein tyrosine kinase activity TAS enables MF false ACCEPT WEE1 directly phosphorylates CDK tyrosine. reaction_mf_exact accepted_mf 1 human WEE1 genes/human/WEE1/WEE1-ai-review.yaml WEE1 P30291 NCBITaxon:9606 Homo sapiens 12 R-HSA-170156 WEE1 phosphorylates CCNA:CDK1 yes reactome/R-HSA-170156.md WEE1 phosphorylates CCNA:CDK1 Homo sapiens GO:0004713 protein tyrosine kinase activity TAS enables MF false ACCEPT WEE1 directly phosphorylates CDK tyrosine. reaction_mf_exact accepted_mf 1 human WEE1 genes/human/WEE1/WEE1-ai-review.yaml WEE1 P30291 NCBITaxon:9606 Homo sapiens 13 R-HSA-174164 Phosphorylation of Cyclin A:Cdk2 at Tyr 15 yes reactome/R-HSA-174164.md Phosphorylation of Cyclin A:Cdk2 at Tyr 15 Homo sapiens GO:0004713 protein tyrosine kinase activity TAS enables MF false ACCEPT WEE1 directly phosphorylates CDK tyrosine. reaction_mf_exact accepted_mf 1 human WEE1 genes/human/WEE1/WEE1-ai-review.yaml WEE1 P30291 NCBITaxon:9606 Homo sapiens 14 R-HSA-69195 Phosphorylation of Cyclin E:CDK2 complexes by WEE1 yes reactome/R-HSA-69195.md Phosphorylation of Cyclin E:CDK2 complexes by WEE1 Homo sapiens GO:0004713 protein tyrosine kinase activity TAS enables MF false ACCEPT WEE1 directly phosphorylates CDK tyrosine. reaction_mf_exact accepted_mf 1 human ZNF598 genes/human/ZNF598/ZNF598-ai-review.yaml ZNF598 Q86UK7 NCBITaxon:9606 Homo sapiens 13 R-HSA-9948301 Reactome: ZNF598 ubiquitin transferase activity / cytosol yes reactome/R-HSA-9948301.md ZNF598 K63 polyubiquitinates RPS10 (eS10) and RPS20 (uS10) of the 40S ribosomal subunit Homo sapiens GO:0004842 ubiquitin-protein transferase activity TAS enables MF false ACCEPT Reactome curation of ZNF598's ubiquitin-protein transferase activity, a parent of the specific ubiquitin protein ligase activity it enables. reaction_mf_exact accepted_mf 1 mouse Akt1 genes/mouse/Akt1/Akt1-ai-review.yaml Akt1 P31750 NCBITaxon:10090 Mus musculus 102 R-MMU-1445150 Akt Phosphorylates As160 (Tbc1d4) yes reactome/R-MMU-1445150.md Akt Phosphorylates As160 (Tbc1d4) Mus musculus GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Core AKT1 serine/threonine kinase activity reaction_mf_exact accepted_mf 1 mouse Akt1 genes/mouse/Akt1/Akt1-ai-review.yaml Akt1 P31750 NCBITaxon:10090 Mus musculus 103 R-MMU-9857656 Phosphorylated Akt1 phosphorylates Connexin-43 (Gja1) yes reactome/R-MMU-9857656.md Phosphorylated Akt1 phosphorylates Connexin-43 (Gja1) Mus musculus GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Core AKT1 serine/threonine kinase activity reaction_mf_exact accepted_mf 1 mouse Akt1 genes/mouse/Akt1/Akt1-ai-review.yaml Akt1 P31750 NCBITaxon:10090 Mus musculus 104 R-MMU-9857664 p-T308,S473-Akt1 phosphorylates Itga5 (Integrin alpha5) in Itga5:Itgb1 (Integrin alpha5:Integrin beta1) yes reactome/R-MMU-9857664.md p-T308,S473-Akt1 phosphorylates Itga5 (Integrin alpha5) in Itga5:Itgb1 (Integrin alpha5:Integrin beta1) Mus musculus GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT Core AKT1 serine/threonine kinase activity reaction_mf_exact accepted_mf 1 mouse Cbl genes/mouse/Cbl/Cbl-ai-review.yaml Cbl P22682 NCBITaxon:10090 Mus musculus 94 R-MMU-9763892 p-Y-Cbl autoubiquitinates and multiubiquitinates p-Y-559-Csf12 yes reactome/R-MMU-9763892.md p-Y-Cbl autoubiquitinates and multiubiquitinates p-Y-559-Csf12 Mus musculus GO:0061630 ubiquitin protein ligase activity TAS enables MF false ACCEPT Core catalytic MF: RING-type E3 ligase that recruits E2~ubiquitin via its RING finger and transfers ubiquitin to activated receptor substrates; strongly supported (IDA:MGI plus IBA/IMP/ISS/TAS). reaction_mf_exact accepted_mf 1 mouse Fyn genes/mouse/Fyn/Fyn-ai-review.yaml Fyn P39688 NCBITaxon:10090 Mus musculus 136 R-MMU-3928613 Fyn phosphorylates Nmdar2b yes reactome/R-MMU-3928613.md Fyn phosphorylates Nmdar2b Mus musculus GO:0004713 protein tyrosine kinase activity TAS enables MF false ACCEPT Fyn is a Src-family non-receptor tyrosine kinase (EC 2.7.10.2) that transfers phosphate from ATP to tyrosine residues of substrate proteins; this is its defining catalytic activity. reaction_mf_exact accepted_mf 1 mouse Fyn genes/mouse/Fyn/Fyn-ai-review.yaml Fyn P39688 NCBITaxon:10090 Mus musculus 137 R-MMU-9763891 Src family kinases phosphorylate Cbl in Csf1 dimer:p-Y559-Csf1r dimer:Cbl yes reactome/R-MMU-9763891.md Src family kinases phosphorylate Cbl in Csf1 dimer:p-Y559-Csf1r dimer:Cbl Mus musculus GO:0004713 protein tyrosine kinase activity TAS enables MF false ACCEPT Fyn is a Src-family non-receptor tyrosine kinase (EC 2.7.10.2) that transfers phosphate from ATP to tyrosine residues of substrate proteins; this is its defining catalytic activity. reaction_mf_exact accepted_mf multi_gene_reactome_id 2 mouse Fyn genes/mouse/Fyn/Fyn-ai-review.yaml Fyn P39688 NCBITaxon:10090 Mus musculus 138 R-MMU-983702 Phosphorylation of ITAMs of Ig-alpha (Cd79a) and Ig-beta (Cd79b) yes reactome/R-MMU-983702.md Phosphorylation of ITAMs of Ig-alpha (Cd79a) and Ig-beta (Cd79b) Mus musculus GO:0004713 protein tyrosine kinase activity TAS enables MF false ACCEPT Fyn is a Src-family non-receptor tyrosine kinase (EC 2.7.10.2) that transfers phosphate from ATP to tyrosine residues of substrate proteins; this is its defining catalytic activity. reaction_mf_exact accepted_mf 1 mouse Fyn genes/mouse/Fyn/Fyn-ai-review.yaml Fyn P39688 NCBITaxon:10090 Mus musculus 139 R-NUL-420398 Autophosphorylation of NCAM1 bound Fyn yes reactome/R-NUL-420398.md Autophosphorylation of NCAM1 bound Fyn Mus musculus GO:0004713 protein tyrosine kinase activity TAS enables MF false ACCEPT Fyn is a Src-family non-receptor tyrosine kinase (EC 2.7.10.2) that transfers phosphate from ATP to tyrosine residues of substrate proteins; this is its defining catalytic activity. reaction_mf_exact accepted_mf 1 mouse Hdac1 genes/mouse/Hdac1/Hdac1-ai-review.yaml Hdac1 O09106 NCBITaxon:10090 Mus musculus 151 R-MMU-573376 Reactome pathway annotation yes reactome/R-MMU-573376.md NoRC:intergenic spacer:Hdac:Dnmt complex deacetylates histone H4 and dimethylates lysine-9 of histone H3 in main promoter of the rRNA gene Mus musculus GO:0004407 histone deacetylase activity TAS enables MF false ACCEPT HDAC1 is a zinc-dependent histone deacetylase (EC 3.5.1.98) that catalyzes removal of acetyl groups from N-acetyl-lysine residues on histones H2A, H2B, H3, and H4. This is the core enzymatic function of HDAC1, well established by direct biochemical assays (PMID:10615135, PMID:21960634, PMID:30279482). reaction_mf_exact accepted_mf 1 mouse Jak1 genes/mouse/Jak1/Jak1-ai-review.yaml Jak1 P52332 NCBITaxon:10090 Mus musculus 54 R-MMU-9674908 p-Y-Jak1,2 phosphorylates Stat1,3,5 in Csf3 dimer:2xp-4Y-Csf3r:Lyn:p-Y-Jak1:p-Jak2:p-Tyk2:Stat1,3,5 yes reactome/R-MMU-9674908.md p-Y-Jak1,2 phosphorylates Stat1,3,5 in Csf3 dimer:2xp-4Y-Csf3r:Lyn:p-Y-Jak1:p-Jak2:p-Tyk2:Stat1,3,5 Mus musculus GO:0004713 protein tyrosine kinase activity TAS enables MF false ACCEPT Core JAK1 non-receptor tyrosine kinase activity reaction_mf_exact accepted_mf 1 mouse Jak1 genes/mouse/Jak1/Jak1-ai-review.yaml Jak1 P52332 NCBITaxon:10090 Mus musculus 55 R-MMU-9674921 p-Y-Jak1 phosphorylates Csf3r in Csf3 dimer:2xCsf3r:Lyn:p-Y-Jak1:p-Jak2:p-Tyk2 yes reactome/R-MMU-9674921.md p-Y-Jak1 phosphorylates Csf3r in Csf3 dimer:2xCsf3r:Lyn:p-Y-Jak1:p-Jak2:p-Tyk2 Mus musculus GO:0004713 protein tyrosine kinase activity TAS enables MF false ACCEPT Core JAK1 non-receptor tyrosine kinase activity reaction_mf_exact accepted_mf 1 mouse Mapk1 genes/mouse/Mapk1/Mapk1-ai-review.yaml Mapk1 P63085 NCBITaxon:10090 Mus musculus 68 R-MMU-198706 ERK1/2 activates ELK1 yes reactome/R-MMU-198706.md ERK1/2 activates ELK1 Mus musculus GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT protein serine/threonine kinase activity is consistent with core ERK MAP kinase activity, phosphorylation, cascade function, or localization. reaction_mf_exact accepted_mf multi_gene_reactome_id 2 mouse Mapk1 genes/mouse/Mapk1/Mapk1-ai-review.yaml Mapk1 P63085 NCBITaxon:10090 Mus musculus 69 R-MMU-9769840 Mapk1,(Mapk3) phosphorylate Npas4 yes reactome/R-MMU-9769840.md Mapk1,(Mapk3) phosphorylate Npas4 Mus musculus GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT protein serine/threonine kinase activity is consistent with core ERK MAP kinase activity, phosphorylation, cascade function, or localization. reaction_mf_exact accepted_mf 1 mouse Mapk3 genes/mouse/Mapk3/Mapk3-ai-review.yaml Mapk3 Q63844 NCBITaxon:10090 Mus musculus 55 R-MMU-198706 ERK1/2 activates ELK1 yes reactome/R-MMU-198706.md ERK1/2 activates ELK1 Mus musculus GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT protein serine/threonine kinase activity is consistent with core ERK MAP kinase activity, phosphorylation, cascade function, or localization. reaction_mf_exact accepted_mf multi_gene_reactome_id 2 mouse Src genes/mouse/Src/Src-ai-review.yaml Src P05480 NCBITaxon:10090 Mus musculus 220 R-MMU-3928622 SFKs phosphorylate Git1 yes reactome/R-MMU-3928622.md SFKs phosphorylate Git1 Mus musculus GO:0004713 protein tyrosine kinase activity TAS enables MF false ACCEPT Core SRC non-receptor tyrosine kinase activity reaction_mf_exact accepted_mf 1 mouse Src genes/mouse/Src/Src-ai-review.yaml Src P05480 NCBITaxon:10090 Mus musculus 221 R-MMU-418870 Phosphorylation of Unc5C yes reactome/R-MMU-418870.md Phosphorylation of Unc5C Mus musculus GO:0004713 protein tyrosine kinase activity TAS enables MF false ACCEPT Core SRC non-receptor tyrosine kinase activity reaction_mf_exact accepted_mf 1 mouse Src genes/mouse/Src/Src-ai-review.yaml Src P05480 NCBITaxon:10090 Mus musculus 222 R-MMU-420382 Phosphorylation of FAK by Src kinases yes reactome/R-MMU-420382.md Phosphorylation of FAK by Src kinases Mus musculus GO:0004713 protein tyrosine kinase activity TAS enables MF false ACCEPT Core SRC non-receptor tyrosine kinase activity reaction_mf_exact accepted_mf 1 mouse Src genes/mouse/Src/Src-ai-review.yaml Src P05480 NCBITaxon:10090 Mus musculus 223 R-MMU-9011252 Src phosphorylates Robo3.1 in response to Ntn1 yes reactome/R-MMU-9011252.md Src phosphorylates Robo3.1 in response to Ntn1 Mus musculus GO:0004713 protein tyrosine kinase activity TAS enables MF false ACCEPT Core SRC non-receptor tyrosine kinase activity reaction_mf_exact accepted_mf 1 mouse Src genes/mouse/Src/Src-ai-review.yaml Src P05480 NCBITaxon:10090 Mus musculus 224 R-MMU-9763891 Src family kinases phosphorylate Cbl in Csf1 dimer:p-Y559-Csf1r dimer:Cbl yes reactome/R-MMU-9763891.md Src family kinases phosphorylate Cbl in Csf1 dimer:p-Y559-Csf1r dimer:Cbl Mus musculus GO:0004713 protein tyrosine kinase activity TAS enables MF false ACCEPT Core SRC non-receptor tyrosine kinase activity reaction_mf_exact accepted_mf multi_gene_reactome_id 2 mouse Src genes/mouse/Src/Src-ai-review.yaml Src P05480 NCBITaxon:10090 Mus musculus 225 R-NUL-9726861 Src phosphorylates RHOU yes reactome/R-NUL-9726861.md Src phosphorylates RHOU Homo sapiens GO:0004713 protein tyrosine kinase activity TAS enables MF false ACCEPT Core SRC non-receptor tyrosine kinase activity reaction_mf_exact accepted_mf 1 rat Akt1 genes/rat/Akt1/Akt1-ai-review.yaml Akt1 P47196 NCBITaxon:10116 Rattus norvegicus 24 R-RNO-198601 Akt1 phosphorylates GSK3 yes reactome/R-RNO-198601.md Akt1 phosphorylates GSK3 Rattus norvegicus GO:0004674 protein serine/threonine kinase activity TAS enables MF false ACCEPT The rat ISO traces to mouse Akt1 and human AKT1. The donor annotation has experimental support and represents a conserved core kinase or PI3K/insulin-signaling function. Rat experimental annotations also exist for this term (PMID:10454575, PMID:12089343, PMID:8524413). reaction_mf_exact accepted_mf 1 yeast GET3 genes/yeast/GET3/GET3-ai-review.yaml GET3 Q12154 NCBITaxon:559292 Saccharomyces cerevisiae 43 R-SCE-9628646 SBH1:GET3:ADP binds GET1:GET2 yes reactome/R-SCE-9628646.md SBH1:GET3:ADP binds GET1:GET2 Saccharomyces cerevisiae GO:0016887 ATP hydrolysis activity TAS enables MF false ACCEPT Manual review: ATP hydrolysis activity is consistent with known biology of GET3. reaction_mf_exact accepted_mf 1 human ABHD12 genes/human/ABHD12/ABHD12-ai-review.yaml ABHD12 Q8N2K0 NCBITaxon:9606 Homo sapiens 26 R-HSA-5694462 ABHD6,12 hydrolyse 3AG yes reactome/R-HSA-5694462.md ABHD6,12 hydrolyse 3AG Homo sapiens GO:0047372 monoacylglycerol lipase activity TAS enables MF false KEEP_AS_NON_CORE The Reactome reaction captures ABHD12 hydrolysis of arachidonoylglycerol. reaction_mf_exact accepted_non_core_mf 1 human ACSL4 genes/human/ACSL4/ACSL4-ai-review.yaml ACSL4 O60488 NCBITaxon:9606 Homo sapiens 22 R-HSA-434382 ACSL3,4 ligates coenzyme A (CoA-SH) to a long chain fatty acid yielding fatty acyl-coenzyme A yes reactome/R-HSA-434382.md ACSL3,4 ligates coenzyme A (CoA-SH) to a long chain fatty acid yielding fatty acyl-coenzyme A Homo sapiens GO:0090433 palmitoyl-CoA ligase activity TAS enables MF false KEEP_AS_NON_CORE Palmitate activation is a reported secondary substrate activity. reaction_mf_exact accepted_non_core_mf 1 human ADA genes/human/ADA/ADA-ai-review.yaml ADA P00813 NCBITaxon:9606 Homo sapiens 36 R-HSA-9754964 ADA deamidates RBV yes reactome/R-HSA-9754964.md ADA deamidates RBV Homo sapiens GO:0019239 deaminase activity TAS enables MF false KEEP_AS_NON_CORE Reactome assigns generic deaminase activity to ribavirin deamidation. reaction_mf_exact accepted_non_core_mf 1 human ALDH7A1 genes/human/ALDH7A1/ALDH7A1-ai-review.yaml ALDH7A1 P49419 NCBITaxon:9606 Homo sapiens 14 R-HSA-6797955 ALDH7A1 oxidises BETALD to BET yes reactome/R-HSA-6797955.md ALDH7A1 oxidises BETALD to BET Homo sapiens GO:0008802 betaine-aldehyde dehydrogenase (NAD+) activity TAS enables MF false KEEP_AS_NON_CORE Reactome records the supported betaine-aldehyde reaction. reaction_mf_exact accepted_non_core_mf 1 human ANO10 genes/human/ANO10/ANO10-ai-review.yaml ANO10 Q9NW15 NCBITaxon:9606 Homo sapiens 6 R-HSA-2684901 ANOs transport cytosolic Cl- to extracellular region yes reactome/R-HSA-2684901.md ANOs transport cytosolic Cl- to extracellular region Homo sapiens GO:0005254 chloride channel activity TAS enables MF false KEEP_AS_NON_CORE The historical Reactome channel assignment represents a secondary ion-permeation capability. reaction_mf_exact accepted_non_core_mf multi_gene_reactome_id 2 human ANO5 genes/human/ANO5/ANO5-ai-review.yaml ANO5 Q75V66 NCBITaxon:9606 Homo sapiens 5 R-HSA-2684901 ANOs transport cytosolic Cl- to extracellular region yes reactome/R-HSA-2684901.md ANOs transport cytosolic Cl- to extracellular region Homo sapiens GO:0005254 chloride channel activity TAS enables MF false KEEP_AS_NON_CORE The Reactome channel assertion is retained as a secondary expression-assay activity. reaction_mf_exact accepted_non_core_mf multi_gene_reactome_id 2 human ARIH1 genes/human/ARIH1/ARIH1-ai-review.yaml ARIH1 Q9Y4X5 NCBITaxon:9606 Homo sapiens 23 R-HSA-1169394 ISGylation of IRF3 yes reactome/R-HSA-1169394.md ISGylation of IRF3 Homo sapiens GO:0061662 ISG15 ligase activity TAS enables MF false KEEP_AS_NON_CORE ISG15 ligase activity is retained as a confirmed secondary ubiquitin-like/ISGylation function: UniProt explicitly states that ARIH1 acts as the ligase involved in ISGylation of EIF4E2, while the dominant core function remains ubiquitin transfer with neddylated CRLs. reaction_mf_exact accepted_non_core_mf 1 human ARIH1 genes/human/ARIH1/ARIH1-ai-review.yaml ARIH1 Q9Y4X5 NCBITaxon:9606 Homo sapiens 24 R-HSA-1169395 ISGylation of viral protein NS1 yes reactome/R-HSA-1169395.md ISGylation of viral protein NS1 Homo sapiens GO:0061662 ISG15 ligase activity TAS enables MF false KEEP_AS_NON_CORE ISG15 ligase activity is retained as a confirmed secondary ubiquitin-like/ISGylation function: UniProt explicitly states that ARIH1 acts as the ligase involved in ISGylation of EIF4E2, while the dominant core function remains ubiquitin transfer with neddylated CRLs. reaction_mf_exact accepted_non_core_mf 1 human ARIH1 genes/human/ARIH1/ARIH1-ai-review.yaml ARIH1 Q9Y4X5 NCBITaxon:9606 Homo sapiens 25 R-HSA-1169398 ISGylation of host protein filamin B yes reactome/R-HSA-1169398.md ISGylation of host protein filamin B Homo sapiens GO:0061662 ISG15 ligase activity TAS enables MF false KEEP_AS_NON_CORE ISG15 ligase activity is retained as a confirmed secondary ubiquitin-like/ISGylation function: UniProt explicitly states that ARIH1 acts as the ligase involved in ISGylation of EIF4E2, while the dominant core function remains ubiquitin transfer with neddylated CRLs. reaction_mf_exact accepted_non_core_mf 1 human ARIH1 genes/human/ARIH1/ARIH1-ai-review.yaml ARIH1 Q9Y4X5 NCBITaxon:9606 Homo sapiens 26 R-HSA-1169402 ISGylation of E2 conjugating enzymes yes reactome/R-HSA-1169402.md ISGylation of E2 conjugating enzymes Homo sapiens GO:0061662 ISG15 ligase activity TAS enables MF false KEEP_AS_NON_CORE ISG15 ligase activity is retained as a confirmed secondary ubiquitin-like/ISGylation function: UniProt explicitly states that ARIH1 acts as the ligase involved in ISGylation of EIF4E2, while the dominant core function remains ubiquitin transfer with neddylated CRLs. reaction_mf_exact accepted_non_core_mf 1 human ARIH1 genes/human/ARIH1/ARIH1-ai-review.yaml ARIH1 Q9Y4X5 NCBITaxon:9606 Homo sapiens 27 R-HSA-1169405 ISGylation of protein phosphatase 1 beta (PP2CB) yes reactome/R-HSA-1169405.md ISGylation of protein phosphatase 1 beta (PP2CB) Homo sapiens GO:0061662 ISG15 ligase activity TAS enables MF false KEEP_AS_NON_CORE ISG15 ligase activity is retained as a confirmed secondary ubiquitin-like/ISGylation function: UniProt explicitly states that ARIH1 acts as the ligase involved in ISGylation of EIF4E2, while the dominant core function remains ubiquitin transfer with neddylated CRLs. reaction_mf_exact accepted_non_core_mf 1 human ARIH1 genes/human/ARIH1/ARIH1-ai-review.yaml ARIH1 Q9Y4X5 NCBITaxon:9606 Homo sapiens 28 R-HSA-1169406 ISGylation of host proteins yes reactome/R-HSA-1169406.md ISGylation of host proteins Homo sapiens GO:0061662 ISG15 ligase activity TAS enables MF false KEEP_AS_NON_CORE ISG15 ligase activity is retained as a confirmed secondary ubiquitin-like/ISGylation function: UniProt explicitly states that ARIH1 acts as the ligase involved in ISGylation of EIF4E2, while the dominant core function remains ubiquitin transfer with neddylated CRLs. reaction_mf_exact accepted_non_core_mf 1 human ARIH1 genes/human/ARIH1/ARIH1-ai-review.yaml ARIH1 Q9Y4X5 NCBITaxon:9606 Homo sapiens 29 R-HSA-9833973 ISGylation of PKR yes reactome/R-HSA-9833973.md ISGylation of PKR Homo sapiens GO:0061662 ISG15 ligase activity TAS enables MF false KEEP_AS_NON_CORE ISG15 ligase activity is retained as a confirmed secondary ubiquitin-like/ISGylation function: UniProt explicitly states that ARIH1 acts as the ligase involved in ISGylation of EIF4E2, while the dominant core function remains ubiquitin transfer with neddylated CRLs. reaction_mf_exact accepted_non_core_mf 1 human ATP13A2 genes/human/ATP13A2/ATP13A2-ai-review.yaml ATP13A2 Q9NQ11 NCBITaxon:9606 Homo sapiens 64 R-HSA-5692480 ATP13A2 transports cations from cytosol to lysosomal lumen yes reactome/R-HSA-5692480.md ATP13A2 transports cations from cytosol to lysosomal lumen Homo sapiens GO:0019829 ATPase-coupled monoatomic cation transmembrane transporter activity TAS enables MF false KEEP_AS_NON_CORE Retain the reported potassium-associated transport activity as non-core. reaction_mf_exact accepted_non_core_mf 1 human FAR1 genes/human/FAR1/FAR1-ai-review.yaml FAR1 Q8WVX9 NCBITaxon:9606 Homo sapiens 18 R-HSA-390425 FAR1 reduces PalmCoA to HXOL yes reactome/R-HSA-390425.md FAR1 reduces PalmCoA to HXOL Homo sapiens GO:0016491 oxidoreductase activity TAS enables MF false KEEP_AS_NON_CORE FAR1 is an oxidoreductase; this Reactome-asserted parent term is correct but very generic. The specific molecular function (GO:0102965) is preferred; keep this general term as non-core. reaction_mf_exact accepted_non_core_mf 1 human FN3K genes/human/FN3K/FN3K-ai-review.yaml FN3K Q9H479 NCBITaxon:9606 Homo sapiens 12 R-HSA-6788867 FN3K phosphorylates ketosamines yes reactome/R-HSA-6788867.md FN3K phosphorylates ketosamines Homo sapiens GO:0016301 kinase activity TAS enables MF false KEEP_AS_NON_CORE Third annotation to the same generic term. Correct but too general - the specific protein-fructosamine 3-kinase activity (GO:0102194) should be preferred. Keeping consistent with other kinase activity annotations. reaction_mf_exact accepted_non_core_mf 1 human GAA genes/human/GAA/GAA-ai-review.yaml GAA P10253 NCBITaxon:9606 Homo sapiens 20 R-HSA-9036729 Defective GAA does not hydrolyze lysosomal glycogen no GO:0090599 alpha-glucosidase activity TAS enables MF false KEEP_AS_NON_CORE Reactome traceable assertion of alpha-glucosidase activity (in the module describing defective GAA that does not hydrolyse lysosomal glycogen). reaction_mf_exact accepted_non_core_mf reactome_disease_event|uncached_reactome 1 human LPCAT2 genes/human/LPCAT2/LPCAT2-ai-review.yaml LPCAT2 Q7L5N7 NCBITaxon:9606 Homo sapiens 13 R-HSA-1482533 2-acyl LPC is acylated to PC by LPCAT yes reactome/R-HSA-1482533.md 2-acyl LPC is acylated to PC by LPCAT Homo sapiens GO:0047144 2-acylglycerol-3-phosphate O-acyltransferase activity TAS enables MF false KEEP_AS_NON_CORE This annotation suggests LPCAT2 can acylate 2-acyl-sn-glycero-3-phosphate (sn-1 acylation). While LPCAT2 primarily acylates at sn-2 position, this represents a less well-characterized activity. reaction_mf_exact accepted_non_core_mf multi_gene_reactome_id 4 human LRP8 genes/human/LRP8/LRP8-ai-review.yaml LRP8 Q14114 NCBITaxon:9606 Homo sapiens 38 R-HSA-2404131 LRPs transport extracellular CR:atREs:HSPG:apoE to cytosol yes reactome/R-HSA-2404131.md LRPs transport extracellular CR:atREs:HSPG:apoE to cytosol Homo sapiens GO:0005041 low-density lipoprotein particle receptor activity TAS enables MF false KEEP_AS_NON_CORE TAS annotation of low-density lipoprotein particle receptor activity reviewed against the cached source, UniProt record, and LRP8-specific biology. reaction_mf_exact accepted_non_core_mf multi_gene_reactome_id 3 human PGK1 genes/human/PGK1/PGK1-ai-review.yaml PGK1 P00558 NCBITaxon:9606 Homo sapiens 28 R-HSA-9975250 PGK1 phosphorylates AKT1S1 yes reactome/R-HSA-9975250.md PGK1 phosphorylates AKT1S1 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false KEEP_AS_NON_CORE Reactome traceable-author-statement annotation of the moonlighting protein serine/threonine kinase activity (PGK1 phosphorylating AKT1S1/PRAS40). reaction_mf_exact accepted_non_core_mf 1 human PIGG genes/human/PIGG/PIGG-ai-review.yaml PIGG Q5H8A4 NCBITaxon:9606 Homo sapiens 13 R-HSA-162742 (ethanolamineP) mannose (a1-2) mannose (a1-6) (ethanolamineP) mannose (a1-4) glucosaminyl-acyl-PI -> (ethanolamineP) mannose (a1-2) (ethanolamineP) mannose (a1-6) (ethanolamineP) mannose (a1-4) glucosaminyl-acyl-PI yes reactome/R-HSA-162742.md (ethanolamineP) mannose (a1-2) mannose (a1-6) (ethanolamineP) mannose (a1-4) glucosaminyl-acyl-PI -> (ethanolamineP) mannose (a1-2) (ethanolamineP) mannose (a1-6) (ethanolamineP) mannose (a1-4) glucosaminyl-acyl-PI Homo sapiens GO:0016780 phosphotransferase activity, for other substituted phosphate groups TAS enables MF false KEEP_AS_NON_CORE Reactome traceable assertion capturing PIGG's phosphotransferase (EtNP-transfer) reaction at a broader level. This is a correct parent of the specific mannose-ethanolamine phosphotransferase activity but is less informative than GO:0051377. reaction_mf_exact accepted_non_core_mf local_module_mf 1 gpi_anchor_ethanolamine_phosphate:GO:0051377 mannose-ethanolamine phosphotransferase activity human PIP5K1B genes/human/PIP5K1B/PIP5K1B-ai-review.yaml PIP5K1B O14986 NCBITaxon:9606 Homo sapiens 11 R-HSA-1676134 PI3P is phosphorylated to PI(3,5)P2 by PIP5K1A/B at the plasma membrane yes reactome/R-HSA-1676134.md PI3P is phosphorylated to PI(3,5)P2 by PIP5K1A/B at the plasma membrane Homo sapiens GO:0000285 1-phosphatidylinositol-3-phosphate 5-kinase activity TAS enables MF false KEEP_AS_NON_CORE Reactome assigns this side-reaction/substrate context to PIP5K1B, but it is not the defining physiological activity. The falcon deep research notes that PI3P phosphorylation by type I PIP5Ks is observed in vitro, while PI4P remains the predominant cellular substrate. reaction_mf_exact accepted_non_core_mf 1 human PIP5K1B genes/human/PIP5K1B/PIP5K1B-ai-review.yaml PIP5K1B O14986 NCBITaxon:9606 Homo sapiens 13 R-HSA-1675810 PI is phosphorylated to PI5P by PIP5K1A/B at the plasma membrane yes reactome/R-HSA-1675810.md PI is phosphorylated to PI5P by PIP5K1A/B at the plasma membrane Homo sapiens GO:0052810 1-phosphatidylinositol-5-kinase activity TAS enables MF false KEEP_AS_NON_CORE Reactome assigns this side-reaction/substrate context to PIP5K1B, but it is not the defining physiological activity. reaction_mf_exact accepted_non_core_mf 1 human SIRT5 genes/human/SIRT5/SIRT5-ai-review.yaml SIRT5 Q9NXA8 NCBITaxon:9606 Homo sapiens 38 R-HSA-5688294 SIRT5 deacetylates Cytochrome C yes reactome/R-HSA-5688294.md SIRT5 deacetylates Cytochrome C Homo sapiens GO:0034979 NAD-dependent protein lysine deacetylase activity TAS enables MF false KEEP_AS_NON_CORE Purified human SIRT5 has weak NAD-dependent deacetylation, substantially less efficient than its acidic deacylation activities. reaction_mf_exact accepted_non_core_mf 1 human SLC25A1 genes/human/SLC25A1/SLC25A1-ai-review.yaml SLC25A1 P53007 NCBITaxon:9606 Homo sapiens 13 R-HSA-372449 SLC25A1 may exchange mitochondrial PEP for cytosolic anion yes reactome/R-HSA-372449.md SLC25A1 may exchange mitochondrial PEP for cytosolic anion Homo sapiens GO:0015142 tricarboxylic acid transmembrane transporter activity TAS enables MF false KEEP_AS_NON_CORE Reactome (TAS) annotation to tricarboxylic acid transmembrane transporter activity, from the hypothetical PEP/citrate exchange reaction. This is a correct broader parent of the specific citrate transporter activity; kept as a non-core (true parent) molecular function. reaction_mf_exact accepted_non_core_mf 1 human SLC25A1 genes/human/SLC25A1/SLC25A1-ai-review.yaml SLC25A1 P53007 NCBITaxon:9606 Homo sapiens 14 R-HSA-75849 SLC25A1 exchanges CIT and MAL yes reactome/R-HSA-75849.md SLC25A1 exchanges CIT and MAL Homo sapiens GO:0015142 tricarboxylic acid transmembrane transporter activity TAS enables MF false KEEP_AS_NON_CORE Reactome (TAS) annotation to tricarboxylic acid transmembrane transporter activity, from the SLC25A1 citrate/malate exchange reaction. Correct broader parent of the specific citrate transporter activity; kept as a non-core (true parent) molecular function. reaction_mf_exact accepted_non_core_mf 1 human SLC25A10 genes/human/SLC25A10/SLC25A10-ai-review.yaml SLC25A10 Q9UBX3 NCBITaxon:9606 Homo sapiens 27 R-HSA-1614546 Sulfate is exported to the cytosol in exchange for dicarboxylate yes reactome/R-HSA-1614546.md Sulfate is exported to the cytosol in exchange for dicarboxylate Homo sapiens GO:0005310 dicarboxylic acid transmembrane transporter activity TAS enables MF false KEEP_AS_NON_CORE Reactome-asserted dicarboxylate transporter activity (sulfate exported in exchange for dicarboxylate/malate). Correct at the general level; the specific MF is the dicarboxylate:phosphate antiporter activity captured in core functions. reaction_mf_exact accepted_non_core_mf 1 human SLC25A32 genes/human/SLC25A32/SLC25A32-ai-review.yaml SLC25A32 Q9H2D1 NCBITaxon:9606 Homo sapiens 17 R-HSA-200680 SLC25A32 transports THF from cytosol to mitochondrial matrix yes reactome/R-HSA-200680.md SLC25A32 transports THF from cytosol to mitochondrial matrix Homo sapiens GO:0008517 folic acid transmembrane transporter activity TAS enables MF false KEEP_AS_NON_CORE Reactome curator-asserted folate (THF) transporter activity, reflecting the original identification of SLC25A32 as the mitochondrial folate transporter. UniProt now notes this was superseded as the primary function by FAD transport, but folate transporter activity remains a documented, curator-supported activity of the protein. Keep as non-core rather than removing an experimental/curator TAS call; the specific FAD activity is the core function. reaction_mf_exact accepted_non_core_mf 1 human SLC25A32 genes/human/SLC25A32/SLC25A32-ai-review.yaml SLC25A32 Q9H2D1 NCBITaxon:9606 Homo sapiens 18 R-HSA-200720 SLC25A32 transports THF from mitochondrial matrix to cytosol yes reactome/R-HSA-200720.md SLC25A32 transports THF from mitochondrial matrix to cytosol Homo sapiens GO:0008517 folic acid transmembrane transporter activity TAS enables MF false KEEP_AS_NON_CORE Duplicate Reactome TAS folate transporter activity annotation (matrix-to-cytosol direction of the same reversible THF transport reaction). Same assessment as R-HSA-200680: real curator assertion, but non-core relative to FAD transport. reaction_mf_exact accepted_non_core_mf 1 human SLC25A4 genes/human/SLC25A4/SLC25A4-ai-review.yaml SLC25A4 P12235 NCBITaxon:9606 Homo sapiens 38 R-HSA-9864415 AAC1 imports a proton yes reactome/R-HSA-9864415.md AAC1 imports a proton Homo sapiens GO:0015078 proton transmembrane transporter activity TAS enables MF false KEEP_AS_NON_CORE "Reactome-curated proton-transport activity (""AAC1 imports a proton""), corresponding to the secondary uncoupling/proton-leak function of ANT. A genuine but non-core molecular function." reaction_mf_exact accepted_non_core_mf 1 human SLC35A1 genes/human/SLC35A1/SLC35A1-ai-review.yaml SLC35A1 P78382 NCBITaxon:9606 Homo sapiens 17 R-HSA-9940804 SLC35A1,4 import CDP-ribitol yes reactome/R-HSA-9940804.md SLC35A1,4 import CDP-ribitol Homo sapiens GO:0015218 pyrimidine nucleotide transmembrane transporter activity TAS enables MF false KEEP_AS_NON_CORE "Reactome annotates this term for the reaction ""SLC35A1,4 import CDP-ribitol"", reflecting the By-similarity CDP-ribitol/CDP exchange activity that contributes CDP-ribitol for matriglycan synthesis. CDP-ribitol/CDP are pyrimidine nucleotides, so the term fits this secondary activity." reaction_mf_exact accepted_non_core_mf 1 human SLC6A6 genes/human/SLC6A6/SLC6A6-ai-review.yaml SLC6A6 P31641 NCBITaxon:9606 Homo sapiens 61 R-HSA-351987 SLC6A6-mediated uptake of taurine and beta-alanine yes reactome/R-HSA-351987.md SLC6A6-mediated uptake of taurine and beta-alanine Homo sapiens GO:0015171 amino acid transmembrane transporter activity TAS enables MF false KEEP_AS_NON_CORE Reactome curator statement (SLC6A6-mediated uptake of taurine and beta-alanine). Correct but a general parent of the taurine-specific activity; non-core generalization. reaction_mf_exact accepted_non_core_mf 1 human SRD5A3 genes/human/SRD5A3/SRD5A3-ai-review.yaml SRD5A3 Q9H8P0 NCBITaxon:9606 Homo sapiens 21 R-HSA-4755572 Defective SRD5A3 does not reduce pPNOL to DCHOL yes reactome/R-HSA-4755572.md Defective SRD5A3 does not reduce pPNOL to DCHOL Homo sapiens GO:0016628 oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor TAS enables MF false KEEP_AS_NON_CORE Reactome-derived high-level oxidoreductase MF annotation. Correct as a parent class of the polyprenal reductase reaction (NADPH-dependent reduction of a C=C bond) but general. reaction_mf_exact accepted_non_core_mf reactome_disease_event 1 mouse Dnmt1 genes/mouse/Dnmt1/Dnmt1-ai-review.yaml Dnmt1 P13864 NCBITaxon:10090 Mus musculus 87 R-MMU-573383 NoRC:intergenic spacer:Hdac:Dnmt complex methylates cytosine in the rRNA genes yes reactome/R-MMU-573383.md NoRC:intergenic spacer:Hdac:Dnmt complex methylates cytosine in the rRNA genes Mus musculus GO:0009008 DNA-methyltransferase activity TAS enables MF false KEEP_AS_NON_CORE DNA-methyltransferase activity reaction_mf_exact accepted_non_core_mf 1 mouse Dnmt1 genes/mouse/Dnmt1/Dnmt1-ai-review.yaml Dnmt1 P13864 NCBITaxon:10090 Mus musculus 88 R-MMU-5336369 Dnmt1 methylates cytosine in hemimethylated DNA yes reactome/R-MMU-5336369.md Dnmt1 methylates cytosine in hemimethylated DNA Mus musculus GO:0051718 DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates TAS enables MF false KEEP_AS_NON_CORE DNA (cytosine-5-)-methyltransferase acti... reaction_mf_exact accepted_non_core_mf 1 rat Tst genes/rat/Tst/Tst-ai-review.yaml Tst P24329 NCBITaxon:10116 Rattus norvegicus 9 R-RNO-1614611 Persulfide sulfur is transferred onto sulfite no GO:0016783 sulfurtransferase activity TAS enables MF false KEEP_AS_NON_CORE sulfurtransferase activity is the direct parent of the more specific GO:0004792 (thiosulfate-cyanide sulfurtransferase activity) already ACCEPTed for Tst; retained as non-core because the specific child captures the molecular function (TAS, Reactome:R-RNO-1614611). reaction_mf_exact accepted_non_core_mf uncached_reactome 1 human ARHGAP11B genes/human/ARHGAP11B/ARHGAP11B-ai-review.yaml ARHGAP11B Q3KRB8 NCBITaxon:9606 Homo sapiens 3 R-HSA-8981637 RHOA GAPs stimulate RHOA GTPase activity yes reactome/R-HSA-8981637.md RHOA GAPs stimulate RHOA GTPase activity Homo sapiens GO:0005096 GTPase activator activity TAS enables MF false REMOVE Reactome asserts, by TAS, the exact molecular function that two independent IDA annotations on this same gene explicitly negate. GOA therefore ships a flat contradiction inside one gene's record, and the positive side carries the weaker evidence code. activity_contradicted activity_contradicted activity_contradicted activity_contradicted The positive RHOA GAP assignment is explicitly negated by stronger direct annotations on the same gene. manual_verdict|multi_gene_reactome_id 5 human AZIN2 genes/human/AZIN2/AZIN2-ai-review.yaml AZIN2 Q96A70 NCBITaxon:9606 Homo sapiens 28 R-HSA-350598 Reactome pathway yes reactome/R-HSA-350598.md Arginine<=>Agmatine+CO2 Homo sapiens GO:0008792 arginine decarboxylase activity TAS enables MF false REMOVE Legacy positive arginine decarboxylase activity (TAS, Reactome). The CAUTION notes AZIN2 was initially reported to have ODC/ADC activity but the ortholog has neither. activity_contradicted activity_contradicted activity_contradicted activity_contradicted The legacy arginine decarboxylase assignment is contradicted by later evidence that AZIN2 lacks ODC and ADC activity. manual_verdict 1 human LPCAT4 genes/human/LPCAT4/LPCAT4-ai-review.yaml LPCAT4 Q643R3 NCBITaxon:9606 Homo sapiens 27 R-HSA-75885 1-acyl LPA is acylated to PA by AGPAT (LPAAT) yes reactome/R-HSA-75885.md 1-acyl LPA is acylated to PA by AGPAT (LPAAT) Homo sapiens GO:0003841 1-acylglycerol-3-phosphate O-acyltransferase activity TAS enables MF false REMOVE The Reactome LPA-to-PA activity is contradicted by direct human LPCAT4 biochemistry. activity_contradicted activity_contradicted activity_contradicted activity_contradicted Direct human LPCAT4 biochemistry contradicts the family LPA-to-PA activity. manual_verdict|multi_gene_reactome_id 3 human LPCAT4 genes/human/LPCAT4/LPCAT4-ai-review.yaml LPCAT4 Q643R3 NCBITaxon:9606 Homo sapiens 29 R-HSA-1482635 2-acyl LPG is acylated to PG by LPGAT yes reactome/R-HSA-1482635.md 2-acyl LPG is acylated to PG by LPGAT Homo sapiens GO:0047144 2-acylglycerol-3-phosphate O-acyltransferase activity TAS enables MF false REMOVE The 2-acyl LPG reaction is contradicted by position-resolved LPCAT4 substrate testing. activity_contradicted activity_contradicted activity_contradicted activity_contradicted Position-resolved substrate testing contradicts the 2-acyl-LPG family event for LPCAT4. manual_verdict|multi_gene_reactome_id 3 mouse Pld4 genes/mouse/Pld4/Pld4-ai-review.yaml Pld4 Q8BG07 NCBITaxon:10090 Mus musculus 22 R-MMU-1483172 PC is transphosphatidylated to PG by Pld1-4/6 yes reactome/R-MMU-1483172.md PC is transphosphatidylated to PG by Pld1-4/6 Mus musculus GO:0004630 D-type glycerophospholipase activity TAS enables MF false REMOVE This is a positive Reactome annotation to phospholipase D activity, but experimental evidence shows PLD4 lacks authentic PLD activity. activity_contradicted activity_contradicted activity_contradicted activity_contradicted Experimental evidence shows PLD4 lacks authentic PLD activity, contradicting the broad Pld1-4/6 Reactome family event. manual_verdict 1 human B4GALT5 genes/human/B4GALT5/B4GALT5-ai-review.yaml B4GALT5 O43286 NCBITaxon:9606 Homo sapiens 23 R-HSA-1964501 Addition of galactose to Core 6 glycoprotein yes reactome/R-HSA-1964501.md Addition of galactose to Core 6 glycoprotein Homo sapiens GO:0008378 galactosyltransferase activity TAS enables MF false MARK_AS_OVER_ANNOTATED "Reactome TAS to the general galactosyltransferase activity (via the ""Addition of galactose to Core 6 glycoprotein"" reaction). Correct at the family level but subsumed by the specific LacCer-synthase (GO:0008489) and beta-1,4-galactosyltransferase (GO:0003831) terms." reaction_mf_refine broad_parent_or_duplicate reaction_mf_refine broad_parent_or_duplicate Reactome supports a galactosyltransferase reaction, but the row uses a generic parent that is less informative than LacCer synthase or beta-1,4-galactosyltransferase terms. manual_verdict 1 human CYP19A1 genes/human/CYP19A1/CYP19A1-ai-review.yaml CYP19A1 P11511 NCBITaxon:9606 Homo sapiens 22 R-HSA-193060 CYP19A1 hydroxylates ANDST to E1 yes reactome/R-HSA-193060.md CYP19A1 hydroxylates ANDST to E1 Homo sapiens GO:0008395 steroid hydroxylase activity TAS enables MF false MARK_AS_OVER_ANNOTATED Steroid hydroxylase activity is a correct general parent describing the first two (C19 hydroxylation) steps of the aromatase reaction, but it is over-general relative to the specific aromatase activity (GO:0070330). reaction_mf_refine broad_parent_or_duplicate reaction_mf_refine broad_parent_or_duplicate The steroid hydroxylase parent is mechanistically true for the first C19 hydroxylation step but is over-general relative to aromatase activity. manual_verdict|local_module_mf 1 sex_steroid_biosynthesis:GO:0070330 aromatase activity human CYP19A1 genes/human/CYP19A1/CYP19A1-ai-review.yaml CYP19A1 P11511 NCBITaxon:9606 Homo sapiens 23 R-HSA-193143 CYP19A1 hydroxylates TEST to EST17b yes reactome/R-HSA-193143.md CYP19A1 hydroxylates TEST to EST17b Homo sapiens GO:0008395 steroid hydroxylase activity TAS enables MF false MARK_AS_OVER_ANNOTATED Duplicate Reactome annotation of the general steroid hydroxylase parent (for the testosterone -> estradiol reaction); over-general relative to aromatase activity. reaction_mf_refine broad_parent_or_duplicate reaction_mf_refine broad_parent_or_duplicate The steroid hydroxylase parent is a duplicate broad parent for the testosterone-to-estradiol Reactome reaction. manual_verdict|local_module_mf 1 sex_steroid_biosynthesis:GO:0070330 aromatase activity human CYP19A1 genes/human/CYP19A1/CYP19A1-ai-review.yaml CYP19A1 P11511 NCBITaxon:9606 Homo sapiens 24 R-HSA-5601849 Defective CYP19A1 does not convert ANDST to E1 yes reactome/R-HSA-5601849.md Defective CYP19A1 does not convert ANDST to E1 Homo sapiens GO:0008395 steroid hydroxylase activity TAS enables MF false MARK_AS_OVER_ANNOTATED Duplicate Reactome annotation of the general steroid hydroxylase parent (defective-aromatase pathway); over-general relative to the specific aromatase activity. reaction_mf_refine broad_parent_or_duplicate reaction_mf_refine broad_parent_or_duplicate The disease event restates the same broad steroid hydroxylase parent instead of the specific aromatase MF. manual_verdict|reactome_disease_event 1 human CYP51A1 genes/human/CYP51A1/CYP51A1-ai-review.yaml CYP51A1 Q16850 NCBITaxon:9606 Homo sapiens 15 R-HSA-194678 CYP51A1 demethylates LAN yes reactome/R-HSA-194678.md CYP51A1 demethylates LAN Homo sapiens GO:0016712 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen TAS enables MF false MARK_AS_OVER_ANNOTATED Reactome TAS annotation to a mechanistic parent term describing the P450 monooxygenase reaction coupled to a flavoprotein (NADPH-cytochrome P450 reductase) donor. Correct mechanistically but less specific than GO:0008398. reaction_mf_refine broad_parent_or_duplicate reaction_mf_refine broad_parent_or_duplicate The P450 monooxygenase parent is mechanistically sound but less specific than sterol 14-demethylase activity. manual_verdict|local_module_mf 1 cholesterol_synthesis_post_lanosterol:GO:0008398 sterol 14-demethylase activity human DBT genes/human/DBT/DBT-ai-review.yaml DBT P11182 NCBITaxon:9606 Homo sapiens 25 R-HSA-9865115 DBT loss-of-function mutants don't synthesize BCAA-CoA no GO:0016747 acyltransferase activity, transferring groups other than amino-acyl groups TAS enables MF false MARK_AS_OVER_ANNOTATED Broad acyltransferase parent term from Reactome (DBT loss-of-function reaction). reaction_mf_refine broad_parent_or_duplicate reaction_mf_refine broad_parent_or_duplicate The disease event supports DBT acyltransferase chemistry only at a broad parent level. manual_verdict|uncached_reactome 1 human DBT genes/human/DBT/DBT-ai-review.yaml DBT P11182 NCBITaxon:9606 Homo sapiens 31 R-HSA-9859163 DBT transfers BCAA to CoA no GO:0016747 acyltransferase activity, transferring groups other than amino-acyl groups TAS enables MF false MARK_AS_OVER_ANNOTATED Broad acyltransferase parent term from Reactome. reaction_mf_refine broad_parent_or_duplicate reaction_mf_refine broad_parent_or_duplicate The normal BCAA-to-CoA event supports DBT acyltransferase chemistry only at a broad parent level. manual_verdict|uncached_reactome 1 human FAR2 genes/human/FAR2/FAR2-ai-review.yaml FAR2 Q96K12 NCBITaxon:9606 Homo sapiens 10 R-HSA-390438 FAR2 reduces PalmCoA to HXOL yes reactome/R-HSA-390438.md FAR2 reduces PalmCoA to HXOL Homo sapiens GO:0016491 oxidoreductase activity TAS enables MF false MARK_AS_OVER_ANNOTATED Correct but overly general. FAR2 is an oxidoreductase, but the specific alcohol-forming fatty acyl-CoA reductase terms (GO:0080019, GO:0102965) capture its function precisely. Flagged as over-annotation, not removed. reaction_mf_refine broad_parent_or_duplicate reaction_mf_refine broad_parent_or_duplicate The oxidoreductase parent is chemically correct but much broader than fatty acyl-CoA reductase activity. manual_verdict 1 human GAMT genes/human/GAMT/GAMT-ai-review.yaml GAMT Q14353 NCBITaxon:9606 Homo sapiens 11 R-HSA-71286 guanidinoacetate + S-adenosylmethionine => creatine + S-adenosylhomocysteine yes reactome/R-HSA-71286.md guanidinoacetate + S-adenosylmethionine => creatine + S-adenosylhomocysteine Homo sapiens GO:0008168 methyltransferase activity TAS enables MF false MARK_AS_OVER_ANNOTATED Generic methyltransferase activity is correct but is a broad parent of the specific guanidinoacetate N-methyltransferase activity that is separately annotated. reaction_mf_refine broad_parent_or_duplicate reaction_mf_refine broad_parent_or_duplicate The methyltransferase parent is correct but broader than guanidinoacetate N-methyltransferase activity. manual_verdict|local_module_mf 1 creatine_biosynthesis:GO:0030731 guanidinoacetate N-methyltransferase activity human LRAT genes/human/LRAT/LRAT-ai-review.yaml LRAT O95237 NCBITaxon:9606 Homo sapiens 23 R-HSA-2404137 LRAT esterifies RBP1:atROL and FACYLs to atREs yes reactome/R-HSA-2404137.md LRAT esterifies RBP1:atROL and FACYLs to atREs Homo sapiens GO:0016416 O-palmitoyltransferase activity TAS enables MF false MARK_AS_OVER_ANNOTATED Reactome TAS annotation to O-palmitoyltransferase activity. Palmitoyl is one of the acyl groups LRAT transfers (retinyl palmitate is the major product), so this is not wrong, but GO:0016416 is a less informative sibling of the precise GO:0047173 phosphatidylcholine-retinol O-acyltransferase activity and does not capture that the acyl donor is lecithin or that the acceptor is retinol. Over-annotation relative to the specific term. reaction_mf_refine broad_parent_or_duplicate reaction_mf_refine broad_parent_or_duplicate The palmitoyltransferase row captures one LRAT acyl group but is a less informative sibling of phosphatidylcholine-retinol O-acyltransferase. manual_verdict 1 human LRAT genes/human/LRAT/LRAT-ai-review.yaml LRAT O95237 NCBITaxon:9606 Homo sapiens 24 R-HSA-2453855 LRAT esterifies RBP1:atROL and FACYLs to atREs yes reactome/R-HSA-2453855.md LRAT esterifies RBP1:atROL and FACYLs to atREs Homo sapiens GO:0016416 O-palmitoyltransferase activity TAS enables MF false MARK_AS_OVER_ANNOTATED Reactome TAS annotation to O-palmitoyltransferase activity (duplicate of the R-HSA-2404137 annotation). Palmitoyl transfer is part of the LRAT reaction but this generic term is less informative than the specific GO:0047173. Over-annotation relative to the specific term. reaction_mf_refine broad_parent_or_duplicate reaction_mf_refine broad_parent_or_duplicate This duplicates the same broad palmitoyltransferase mapping for LRAT. manual_verdict 1 human LRAT genes/human/LRAT/LRAT-ai-review.yaml LRAT O95237 NCBITaxon:9606 Homo sapiens 25 R-HSA-2466710 Defective LRAT does not esterify RBP1:atROL and FACYLs to atREs yes reactome/R-HSA-2466710.md Defective LRAT does not esterify RBP1:atROL and FACYLs to atREs Homo sapiens GO:0016416 O-palmitoyltransferase activity TAS enables MF false MARK_AS_OVER_ANNOTATED "Reactome TAS annotation to O-palmitoyltransferase activity (from the ""Defective LRAT"" reaction). Same generic palmitoyltransferase term; less informative than the specific GO:0047173. Over-annotation relative to the specific term." reaction_mf_refine broad_parent_or_duplicate reaction_mf_refine broad_parent_or_duplicate The disease event repeats the same broad palmitoyltransferase mapping for LRAT. manual_verdict|reactome_disease_event 1 human MAP3K5 genes/human/MAP3K5/MAP3K5-ai-review.yaml MAP3K5 Q99683 NCBITaxon:9606 Homo sapiens 62 R-HSA-3228469 MAP3K5 phosphorylates MKK3 and MKK6 yes reactome/R-HSA-3228469.md MAP3K5 phosphorylates MKK3 and MKK6 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false MARK_AS_OVER_ANNOTATED "Reactome pathway ""MAP3K5 phosphorylates MKK3 and MKK6."" This TAS annotation confirms kinase activity. Redundant with MAP3K activity." reaction_mf_refine broad_parent_or_duplicate reaction_mf_refine broad_parent_or_duplicate The protein kinase parent is redundant with a more informative MAP3K activity. manual_verdict 1 human MAP3K5 genes/human/MAP3K5/MAP3K5-ai-review.yaml MAP3K5 Q99683 NCBITaxon:9606 Homo sapiens 63 R-NUL-3299417 MAP3K5 (ASK1) phosphorylates Map2k4(Sek1) yes reactome/R-NUL-3299417.md MAP3K5 (ASK1) phosphorylates Map2k4(Sek1) Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false MARK_AS_OVER_ANNOTATED "Reactome pathway ""MAP3K5 (ASK1) phosphorylates Map2k4(Sek1)."" Duplicate TAS annotation for kinase activity. Redundant with MAP3K activity." reaction_mf_refine broad_parent_or_duplicate reaction_mf_refine broad_parent_or_duplicate This is a duplicate generic kinase parent for the MAP3K5 activity. manual_verdict 1 human PIGW genes/human/PIGW/PIGW-ai-review.yaml PIGW Q7Z7B1 NCBITaxon:9606 Homo sapiens 14 R-HSA-162683 glucosaminyl-PI + fatty acyl-CoA -> glucosaminyl-acyl-PI + CoA-SH yes reactome/R-HSA-162683.md glucosaminyl-PI + fatty acyl-CoA -> glucosaminyl-acyl-PI + CoA-SH Homo sapiens GO:0016747 acyltransferase activity, transferring groups other than amino-acyl groups TAS enables MF false MARK_AS_OVER_ANNOTATED Reactome-traceable annotation to a broad acyltransferase-class term for the inositol-acylation reaction (R-HSA-162683). This is a general parent of the specific MF GO:0032216. reaction_mf_refine broad_parent_or_duplicate reaction_mf_refine broad_parent_or_duplicate The acyltransferase parent is correct but broader than the PIGW inositol-acylation MF. manual_verdict|local_module_mf 1 gpi_anchor_core_glycan_assembly:GO:0032216 glucosaminyl-phosphatidylinositol O-acyltransferase activity human RDH11 genes/human/RDH11/RDH11-ai-review.yaml RDH11 Q8TC12 NCBITaxon:9606 Homo sapiens 22 R-HSA-975629 RDH11 reduces RBP2:atRAL to RBP2:atROL yes reactome/R-HSA-975629.md RDH11 reduces RBP2:atRAL to RBP2:atROL Homo sapiens GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor TAS enables MF false MARK_AS_OVER_ANNOTATED High-level oxidoreductase grouping term (CH-OH donor, NAD/NADP acceptor), Reactome TAS. Chemically correct but far too general for a well-characterized retinol dehydrogenase; the specific NADP-dependent retinol dehydrogenase term is more informative. reaction_mf_refine broad_parent_or_duplicate reaction_mf_refine broad_parent_or_duplicate The CH-OH donor oxidoreductase parent is much broader than NADP-dependent retinol dehydrogenase activity. manual_verdict 1 human SPHK1 genes/human/SPHK1/SPHK1-ai-review.yaml SPHK1 Q9NYA1 NCBITaxon:9606 Homo sapiens 54 R-HSA-428273 SPHK1 phosphorylates sphingoid yes reactome/R-HSA-428273.md SPHK1 phosphorylates sphingoid Homo sapiens GO:0001727 lipid kinase activity TAS enables MF false MARK_AS_OVER_ANNOTATED Reactome generic lipid kinase activity. Correct but subsumed by the specific sphingosine kinase activity term. reaction_mf_refine broad_parent_or_duplicate reaction_mf_refine broad_parent_or_duplicate The generic lipid kinase parent is subsumed by sphingosine kinase activity. manual_verdict 1 human SPHK2 genes/human/SPHK2/SPHK2-ai-review.yaml SPHK2 Q9NRA0 NCBITaxon:9606 Homo sapiens 51 R-HSA-9695949 SPHK2 phosphorylates sphingoid yes reactome/R-HSA-9695949.md SPHK2 phosphorylates sphingoid Homo sapiens GO:0001727 lipid kinase activity TAS enables MF false MARK_AS_OVER_ANNOTATED Reactome TAS to the parent 'lipid kinase activity'. Correct but less informative than the specific sphingosine kinase activity term. reaction_mf_refine broad_parent_or_duplicate reaction_mf_refine broad_parent_or_duplicate The generic lipid kinase parent is subsumed by sphingosine kinase activity. manual_verdict 1 human TK2 genes/human/TK2/TK2-ai-review.yaml TK2 O00142 NCBITaxon:9606 Homo sapiens 25 R-HSA-109759 deoxycytidine, thymidine, or deoxyuridine + ATP => dCMP, TMP, or dUMP + ADP [TK2] yes reactome/R-HSA-109759.md deoxycytidine, thymidine, or deoxyuridine + ATP => dCMP, TMP, or dUMP + ADP [TK2] Homo sapiens GO:0019206 nucleoside kinase activity TAS enables MF false MARK_AS_OVER_ANNOTATED Reactome TAS annotation to the broad parent term nucleoside kinase activity for the TK2 reaction (deoxycytidine/thymidine/deoxyuridine + ATP => dCMP/TMP/dUMP + ADP). reaction_mf_refine broad_parent_or_duplicate reaction_mf_refine broad_parent_or_duplicate The nucleoside kinase parent is true but broader than the deoxyribonucleoside kinase activities in the TK2 event. manual_verdict|local_module_mf 1 mitochondrial_dntp_supply:GO:0004797 thymidine kinase activity human MAGT1 genes/human/MAGT1/MAGT1-ai-review.yaml MAGT1 Q9H0U3 NCBITaxon:9606 Homo sapiens 24 R-HSA-5339538 MAGT1 transports Mg2+ from extracellular region to cytosol yes reactome/R-HSA-5339538.md MAGT1 transports Mg2+ from extracellular region to cytosol Homo sapiens GO:0015095 magnesium ion transmembrane transporter activity TAS enables MF false MARK_AS_OVER_ANNOTATED "Over-annotation. Reactome (""MAGT1 transports Mg2+ from extracellular region to cytosol"") encodes the historical direct-transporter model. The best-supported MF is the OST-B oxidoreductase activity; the direct Mg2+-transporter activity is contested and likely indirect. Mark as over-annotated." contested_direct_activity contested_direct_activity contested_direct_activity contested_direct_activity The historical direct Mg2+ transporter model is contested; MAGT1's established activity is the OST-B thioredoxin-like oxidoreductase role. manual_verdict 1 human RFT1 genes/human/RFT1/RFT1-ai-review.yaml RFT1 Q96AA3 NCBITaxon:9606 Homo sapiens 9 R-HSA-4570573 Defective RFT1 does not flip the N-glycan precursor yes reactome/R-HSA-4570573.md Defective RFT1 does not flip the N-glycan precursor Homo sapiens GO:0034202 glycolipid floppase activity TAS enables MF false MARK_AS_OVER_ANNOTATED "TAS from the Reactome disease reaction ""Defective RFT1 does not flip the N-glycan precursor"". Reactome asserts the canonical flippase model, which is a reasonable reading of the genetics but inherits the same ATP-dependence mismatch as the IDA row, and additionally states as settled a mechanism that the primary literature still treats as open." contested_direct_activity contested_direct_activity contested_direct_activity contested_direct_activity The disease event asserts the canonical flippase model, but the direct mechanism and ATP-dependence remain unsettled. manual_verdict|reactome_disease_event 1 human TUSC3 genes/human/TUSC3/TUSC3-ai-review.yaml TUSC3 Q13454 NCBITaxon:9606 Homo sapiens 20 R-HSA-5339528 TUSC3 transports Mg2+ from extracellular region to cytosol yes reactome/R-HSA-5339528.md TUSC3 transports Mg2+ from extracellular region to cytosol Homo sapiens GO:0015095 magnesium ion transmembrane transporter activity TAS enables MF false MARK_AS_OVER_ANNOTATED "Reactome TAS asserting TUSC3 enables magnesium ion transmembrane transporter activity (in a reaction ""TUSC3 transports Mg2+ from extracellular region to cytosol""). TUSC3 has no demonstrated intrinsic transporter/channel activity; its established MF is a thioredoxin-like oxidoreductase." contested_direct_activity contested_direct_activity contested_direct_activity contested_direct_activity TUSC3 has no demonstrated intrinsic Mg2+ channel or transporter activity; its established MF is thioredoxin-like oxidoreductase. manual_verdict 1 KLEPN rmtD genes/KLEPN/rmtD/rmtD-ai-review.yaml rmtD B0F9V0 NCBITaxon:573 Klebsiella pneumoniae 5 R-HSA-9911509 RMTases methylate 16S ribosomal RNA yes reactome/R-HSA-9911509.md RMTases methylate 16S ribosomal RNA Homo sapiens GO:0008649 rRNA methyltransferase activity TAS enables MF false MODIFY The existing MF term is directionally correct but less informative than the family-specific AMR activity rRNA (guanine-N7-)-methyltransferase activity. reaction_mf_refine mf_term_refinement multi_gene_reactome_id 2 KLEPN rmtF genes/KLEPN/rmtF/rmtF-ai-review.yaml rmtF I1YZZ5 NCBITaxon:573 Klebsiella pneumoniae 5 R-HSA-9911509 RMTases methylate 16S ribosomal RNA yes reactome/R-HSA-9911509.md RMTases methylate 16S ribosomal RNA Homo sapiens GO:0008649 rRNA methyltransferase activity TAS enables MF false MODIFY The existing MF term is directionally correct but less informative than the family-specific AMR activity rRNA (guanine-N7-)-methyltransferase activity. reaction_mf_refine mf_term_refinement multi_gene_reactome_id 2 human ABCA4 genes/human/ABCA4/ABCA4-ai-review.yaml ABCA4 P78363 NCBITaxon:9606 Homo sapiens 36 R-HSA-1467466 ABCA4 mediates atRAL transport yes reactome/R-HSA-1467466.md ABCA4 mediates atRAL transport Homo sapiens GO:0034632 retinol transmembrane transporter activity TAS enables MF false MODIFY The Reactome event describes retinal or NRPE handling, but the assigned GO term denotes retinol, the corresponding alcohol. reaction_mf_refine mf_term_refinement 1 human ABCA4 genes/human/ABCA4/ABCA4-ai-review.yaml ABCA4 P78363 NCBITaxon:9606 Homo sapiens 37 R-HSA-2466802 Defective ABCA4 does not transport NRPE from disc membranes yes reactome/R-HSA-2466802.md Defective ABCA4 does not transport NRPE from disc membranes Homo sapiens GO:0034632 retinol transmembrane transporter activity TAS enables MF false MODIFY The Reactome event describes retinal or NRPE handling, but the assigned GO term denotes retinol, the corresponding alcohol. reaction_mf_refine mf_term_refinement reactome_disease_event 1 human ABCB4 genes/human/ABCB4/ABCB4-ai-review.yaml ABCB4 P21439 NCBITaxon:9606 Homo sapiens 59 R-HSA-5678706 ABCB4 transports PC from plasma membrane to extracellular region yes reactome/R-HSA-5678706.md ABCB4 transports PC from plasma membrane to extracellular region Homo sapiens GO:0120014 phospholipid transfer activity TAS enables MF false MODIFY The Reactome PC-export event is executed by a membrane floppase. reaction_mf_refine mf_term_refinement 1 human ABCB4 genes/human/ABCB4/ABCB4-ai-review.yaml ABCB4 P21439 NCBITaxon:9606 Homo sapiens 60 R-HSA-5678749 Defective ABCB4 does not transport PC from plasma membrane to extracellular region yes reactome/R-HSA-5678749.md Defective ABCB4 does not transport PC from plasma membrane to extracellular region Homo sapiens GO:0120014 phospholipid transfer activity TAS enables MF false MODIFY The Reactome PC-export event is executed by a membrane floppase. reaction_mf_refine mf_term_refinement reactome_disease_event 1 human ABCC8 genes/human/ABCC8/ABCC8-ai-review.yaml ABCC8 Q09428 NCBITaxon:9606 Homo sapiens 30 R-HSA-5683209 Activating ABCC8 mutants cause hyperglycemia in permanent neonatal diabetes mellitus (PNDM) and transient neonatal DM (TNDM) yes reactome/R-HSA-5683209.md Activating ABCC8 mutants cause hyperglycemia in permanent neonatal diabetes mellitus (PNDM) and transient neonatal DM (TNDM) Homo sapiens GO:0015272 ATP-activated inward rectifier potassium channel activity TAS enables MF false MODIFY Activating SUR1 variants alter regulation of a separate potassium pore. reaction_mf_refine mf_term_refinement 1 human ABCC9 genes/human/ABCC9/ABCC9-ai-review.yaml ABCC9 O60706 NCBITaxon:9606 Homo sapiens 36 R-HSA-1454916 The ABCC family mediates organic anion transport yes reactome/R-HSA-1454916.md The ABCC family mediates organic anion transport Homo sapiens GO:0042626 ATPase-coupled transmembrane transporter activity TAS enables MF false MODIFY The family-level Reactome mapping assigns active transport too broadly to a nontransporting channel regulator. reaction_mf_refine mf_term_refinement multi_gene_reactome_id 3 human ABCD1 genes/human/ABCD1/ABCD1-ai-review.yaml ABCD1 P33897 NCBITaxon:9606 Homo sapiens 9 R-HSA-5684043 Defective ABCD1 does not transfer LCFAs from cytosol to peroxisomal matrix yes reactome/R-HSA-5684043.md Defective ABCD1 does not transfer LCFAs from cytosol to peroxisomal matrix Homo sapiens GO:0005324 long-chain fatty acid transmembrane transporter activity TAS enables MF false MODIFY ABCD1 recognizes fatty-acyl-CoA substrates for ATP-dependent peroxisomal import. reaction_mf_refine mf_term_refinement reactome_disease_event 1 human AKR1D1 genes/human/AKR1D1/AKR1D1-ai-review.yaml AKR1D1 P51857 NCBITaxon:9606 Homo sapiens 20 R-HSA-192033 4-cholesten-7alpha-ol-3-one is reduced to 5beta-cholestan-7alpha-ol-3-one yes reactome/R-HSA-192033.md 4-cholesten-7alpha-ol-3-one is reduced to 5beta-cholestan-7alpha-ol-3-one Homo sapiens GO:0008106 alcohol dehydrogenase (NADP+) activity TAS enables MF false MODIFY This Reactome reaction is steroid double-bond reduction. reaction_mf_refine mf_term_refinement local_module_mf 1 bile_acid_synthesis_ring_modification:GO:0047787 Delta4-3-oxosteroid 5beta-reductase activity human AKR1D1 genes/human/AKR1D1/AKR1D1-ai-review.yaml AKR1D1 P51857 NCBITaxon:9606 Homo sapiens 21 R-HSA-192067 4-cholesten-7alpha, 12alpha-diol-3-one is reduced to 5beta-cholesten-7alpha, 12alpha-diol-3-one yes reactome/R-HSA-192067.md 4-cholesten-7alpha, 12alpha-diol-3-one is reduced to 5beta-cholesten-7alpha, 12alpha-diol-3-one Homo sapiens GO:0008106 alcohol dehydrogenase (NADP+) activity TAS enables MF false MODIFY This Reactome reaction is steroid double-bond reduction. reaction_mf_refine mf_term_refinement 1 human AKR1D1 genes/human/AKR1D1/AKR1D1-ai-review.yaml AKR1D1 P51857 NCBITaxon:9606 Homo sapiens 22 R-HSA-193746 4-cholesten-7alpha,24(S)-diol-3-one is reduced to 5beta-cholestan-7alpha,24(S)-diol-3-one yes reactome/R-HSA-193746.md 4-cholesten-7alpha,24(S)-diol-3-one is reduced to 5beta-cholestan-7alpha,24(S)-diol-3-one Homo sapiens GO:0008106 alcohol dehydrogenase (NADP+) activity TAS enables MF false MODIFY This Reactome reaction is steroid double-bond reduction. reaction_mf_refine mf_term_refinement 1 human AKR1D1 genes/human/AKR1D1/AKR1D1-ai-review.yaml AKR1D1 P51857 NCBITaxon:9606 Homo sapiens 23 R-HSA-193755 TODO: Fetch title no GO:0008106 alcohol dehydrogenase (NADP+) activity TAS enables MF false MODIFY This Reactome reaction is steroid double-bond reduction. reaction_mf_refine mf_term_refinement uncached_reactome|missing_reactome_title 1 human AKR1D1 genes/human/AKR1D1/AKR1D1-ai-review.yaml AKR1D1 P51857 NCBITaxon:9606 Homo sapiens 24 R-HSA-193821 4-cholesten-7alpha,12alpha,27-triol-3-one is reduced to 5beta-cholestan-7alpha,12alpha,27-triol-3-one yes reactome/R-HSA-193821.md 4-cholesten-7alpha,12alpha,27-triol-3-one is reduced to 5beta-cholestan-7alpha,12alpha,27-triol-3-one Homo sapiens GO:0008106 alcohol dehydrogenase (NADP+) activity TAS enables MF false MODIFY This Reactome reaction is steroid double-bond reduction. reaction_mf_refine mf_term_refinement 1 human AKR1D1 genes/human/AKR1D1/AKR1D1-ai-review.yaml AKR1D1 P51857 NCBITaxon:9606 Homo sapiens 25 R-HSA-193824 4-cholesten-7alpha,27-diol-3-one is reduced to 5beta-cholestan-7alpha,27-diol-3-one yes reactome/R-HSA-193824.md 4-cholesten-7alpha,27-diol-3-one is reduced to 5beta-cholestan-7alpha,27-diol-3-one Homo sapiens GO:0008106 alcohol dehydrogenase (NADP+) activity TAS enables MF false MODIFY This Reactome reaction is steroid double-bond reduction. reaction_mf_refine mf_term_refinement 1 human ALG12 genes/human/ALG12/ALG12-ai-review.yaml ALG12 Q9BV10 NCBITaxon:9606 Homo sapiens 16 R-HSA-446198 ALG12 transfers Man to N-glycan precursor (GlcNAc)2 (Man)7 (PP-Dol)1 yes reactome/R-HSA-446198.md ALG12 transfers Man to N-glycan precursor (GlcNAc)2 (Man)7 (PP-Dol)1 Homo sapiens GO:0000030 mannosyltransferase activity TAS enables MF false MODIFY The Reactome reaction resolves the donor and acceptor more precisely than generic mannosyltransferase activity. reaction_mf_refine mf_term_refinement local_module_mf 1 n_glycan_llo_assembly_lumenal:GO:0052917 dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase activity human ALG12 genes/human/ALG12/ALG12-ai-review.yaml ALG12 Q9BV10 NCBITaxon:9606 Homo sapiens 17 R-HSA-4720497 Defective ALG12 does not add mannose to the N-glycan precursor yes reactome/R-HSA-4720497.md Defective ALG12 does not add mannose to the N-glycan precursor Homo sapiens GO:0000030 mannosyltransferase activity TAS enables MF false MODIFY The disease event identifies the normal ALG12 reaction whose activity is impaired. reaction_mf_refine mf_term_refinement reactome_disease_event 1 human ALG3 genes/human/ALG3/ALG3-ai-review.yaml ALG3 Q92685 NCBITaxon:9606 Homo sapiens 19 R-HSA-446188 ALG3 transfers Man to N-glycan precursor (GlcNAc)2 (Man)5 (PP-Dol)1 yes reactome/R-HSA-446188.md ALG3 transfers Man to N-glycan precursor (GlcNAc)2 (Man)5 (PP-Dol)1 Homo sapiens GO:0000033 alpha-1,3-mannosyltransferase activity TAS enables MF false MODIFY The normal Reactome event resolves the donor and acceptor beyond the broad linkage-specific MF. reaction_mf_refine mf_term_refinement local_module_mf 1 n_glycan_llo_assembly_lumenal:GO:0052925 dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase activity human ALG3 genes/human/ALG3/ALG3-ai-review.yaml ALG3 Q92685 NCBITaxon:9606 Homo sapiens 20 R-HSA-4720473 Defective ALG3 does not add mannose to the N-glycan precursor yes reactome/R-HSA-4720473.md Defective ALG3 does not add mannose to the N-glycan precursor Homo sapiens GO:0000033 alpha-1,3-mannosyltransferase activity TAS enables MF false MODIFY The disease event identifies the normal ALG3 reaction that pathogenic variants impair. reaction_mf_refine mf_term_refinement reactome_disease_event 1 human ALG6 genes/human/ALG6/ALG6-ai-review.yaml ALG6 Q9Y672 NCBITaxon:9606 Homo sapiens 16 R-HSA-446202 Addition of the first glucose to the N-glycan precursor by ALG6 yes reactome/R-HSA-446202.md Addition of the first glucose to the N-glycan precursor by ALG6 Homo sapiens GO:0004583 dolichyl-phosphate-glucose-glycolipid alpha-glucosyltransferase activity TAS enables MF false MODIFY The normal Reactome event explicitly models first-glucose transfer by ALG6. reaction_mf_refine mf_term_refinement local_module_mf 1 n_glycan_llo_assembly_lumenal:GO:0042281 dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase activity human ALG6 genes/human/ALG6/ALG6-ai-review.yaml ALG6 Q9Y672 NCBITaxon:9606 Homo sapiens 17 R-HSA-4724291 Defective ALG6 does not add glucose to the N-glycan precursor yes reactome/R-HSA-4724291.md Defective ALG6 does not add glucose to the N-glycan precursor Homo sapiens GO:0004583 dolichyl-phosphate-glucose-glycolipid alpha-glucosyltransferase activity TAS enables MF false MODIFY The disease Reactome event describes altered ALG6 activity in the same first-glucose reaction. reaction_mf_refine mf_term_refinement reactome_disease_event 1 human ALG8 genes/human/ALG8/ALG8-ai-review.yaml ALG8 Q9BVK2 NCBITaxon:9606 Homo sapiens 19 R-HSA-446189 Addition of a second glucose to the N-glycan precursor by ALG8 yes reactome/R-HSA-446189.md Addition of a second glucose to the N-glycan precursor by ALG8 Homo sapiens GO:0004583 dolichyl-phosphate-glucose-glycolipid alpha-glucosyltransferase activity TAS enables MF false MODIFY The normal Reactome reaction resolves the acceptor-specific ALG8 activity. reaction_mf_refine mf_term_refinement local_module_mf 1 n_glycan_llo_assembly_lumenal:GO:0042283 dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase activity human ALG8 genes/human/ALG8/ALG8-ai-review.yaml ALG8 Q9BVK2 NCBITaxon:9606 Homo sapiens 20 R-HSA-4724330 Defective ALG8 does not add glucose to the N-glycan precursor yes reactome/R-HSA-4724330.md Defective ALG8 does not add glucose to the N-glycan precursor Homo sapiens GO:0004583 dolichyl-phosphate-glucose-glycolipid alpha-glucosyltransferase activity TAS enables MF false MODIFY The disease event describes failure of the normal ALG8 glucosyl-transfer step. reaction_mf_refine mf_term_refinement reactome_disease_event 1 human ALG9 genes/human/ALG9/ALG9-ai-review.yaml ALG9 Q9H6U8 NCBITaxon:9606 Homo sapiens 18 R-HSA-446215 ALG9 transfers Man to N-glycan precursor (GlcNAc)2 (Man)6 (PP-Dol)1 yes reactome/R-HSA-446215.md ALG9 transfers Man to N-glycan precursor (GlcNAc)2 (Man)6 (PP-Dol)1 Homo sapiens GO:0000026 alpha-1,2-mannosyltransferase activity TAS enables MF false MODIFY Refine this event to the specific seventh-mannose transfer. reaction_mf_refine mf_term_refinement local_module_mf 1 n_glycan_llo_assembly_lumenal:GO:0052926 dol-P-Man:Man(6)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase activity human ALG9 genes/human/ALG9/ALG9-ai-review.yaml ALG9 Q9H6U8 NCBITaxon:9606 Homo sapiens 19 R-HSA-446216 ALG9 transfers Man to N-glycan precursor (GlcNAc)2 (Man)8 (PP-Dol)1 yes reactome/R-HSA-446216.md ALG9 transfers Man to N-glycan precursor (GlcNAc)2 (Man)8 (PP-Dol)1 Homo sapiens GO:0000026 alpha-1,2-mannosyltransferase activity TAS enables MF false MODIFY Refine this event to the specific ninth-mannose transfer. reaction_mf_refine mf_term_refinement local_module_mf 1 n_glycan_llo_assembly_lumenal:GO:0052918 dol-P-Man:Man(8)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase activity human ALG9 genes/human/ALG9/ALG9-ai-review.yaml ALG9 Q9H6U8 NCBITaxon:9606 Homo sapiens 20 R-HSA-4720478 Defective ALG9 does not add the seventh mannose to the N-glycan precursor yes reactome/R-HSA-4720478.md Defective ALG9 does not add the seventh mannose to the N-glycan precursor Homo sapiens GO:0000026 alpha-1,2-mannosyltransferase activity TAS enables MF false MODIFY Refine this event to the specific seventh-mannose transfer. reaction_mf_refine mf_term_refinement reactome_disease_event 1 human ALG9 genes/human/ALG9/ALG9-ai-review.yaml ALG9 Q9H6U8 NCBITaxon:9606 Homo sapiens 21 R-HSA-9035514 Defective ALG9 does not add the last mannose to the N-glycan precursor yes reactome/R-HSA-9035514.md Defective ALG9 does not add the last mannose to the N-glycan precursor Homo sapiens GO:0000026 alpha-1,2-mannosyltransferase activity TAS enables MF false MODIFY Refine this event to the specific ninth-mannose transfer. reaction_mf_refine mf_term_refinement reactome_disease_event 1 human ALK genes/human/ALK/ALK-ai-review.yaml ALK Q9UM73 NCBITaxon:9606 Homo sapiens 6 R-HSA-201521 ALK autophosphorylation yes reactome/R-HSA-201521.md ALK autophosphorylation Homo sapiens GO:0004713 protein tyrosine kinase activity TAS enables MF false MODIFY Refine kinase chemistry to the established receptor tyrosine kinase activity. reaction_mf_refine mf_term_refinement 1 human AMDHD1 genes/human/AMDHD1/AMDHD1-ai-review.yaml AMDHD1 Q96NU7 NCBITaxon:9606 Homo sapiens 12 R-HSA-70906 4-imidazolone-5-propanoate + H2O => N-formiminoglutamate yes reactome/R-HSA-70906.md 4-imidazolone-5-propanoate + H2O => N-formiminoglutamate Homo sapiens GO:0016812 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides TAS enables MF false MODIFY Correct reaction class from Reactome (the 4-imidazolone-5-propanoate + H2O reaction) but over-general relative to the specific catalytic term. Replace with GO:0050480 imidazolonepropionase activity. reaction_mf_refine mf_term_refinement 1 human ANKZF1 genes/human/ANKZF1/ANKZF1-ai-review.yaml ANKZF1 Q9H8Y5 NCBITaxon:9606 Homo sapiens 5 R-HSA-9948427 ANKZF1 cleaves the peptidyl-tRNA in VCP hexamer:LTN1:NEMF:60S ribosome subunit:peptidyl-tRNA with K48polyUb-nascent peptide yes reactome/R-HSA-9948427.md ANKZF1 cleaves the peptidyl-tRNA in VCP hexamer:LTN1:NEMF:60S ribosome subunit:peptidyl-tRNA with K48polyUb-nascent peptide Homo sapiens GO:0004521 RNA endonuclease activity TAS enables MF false MODIFY The activity is correct but the more specific term tRNA-specific ribonuclease activity better captures ANKZF1's substrate and reaction. reaction_mf_refine mf_term_refinement 1 human ASH1L genes/human/ASH1L/ASH1L-ai-review.yaml ASH1L Q9NR48 NCBITaxon:9606 Homo sapiens 24 R-HSA-4827383 WHSC1 (KMT3G), NSD1 (KMT3B), SMYD2 (KMT3C) methylate lysine-37 of histone H3 (H3K36) yes reactome/R-HSA-4827383.md WHSC1 (KMT3G), NSD1 (KMT3B), SMYD2 (KMT3C) methylate lysine-37 of histone H3 (H3K36) Homo sapiens GO:0140938 histone H3 methyltransferase activity TAS enables MF false MODIFY Replace broad H3 methyltransferase activity with K36 dimethylation. reaction_mf_refine mf_term_refinement 1 human ASH1L genes/human/ASH1L/ASH1L-ai-review.yaml ASH1L Q9NR48 NCBITaxon:9606 Homo sapiens 25 R-HSA-5638157 WHSC1 (KMT3G), NSD1 (KMT3B), SMYD2 (KMT3C), ASH1L methylate methyl-lysine-37 of histone H3 (H3K36) yes reactome/R-HSA-5638157.md WHSC1 (KMT3G), NSD1 (KMT3B), SMYD2 (KMT3C), ASH1L methylate methyl-lysine-37 of histone H3 (H3K36) Homo sapiens GO:0140938 histone H3 methyltransferase activity TAS enables MF false MODIFY The reaction supports the specific H3K36 dimethyltransferase activity. reaction_mf_refine mf_term_refinement 1 human ATG16L1 genes/human/ATG16L1/ATG16L1-ai-review.yaml ATG16L1 Q676U5 NCBITaxon:9606 Homo sapiens 48 R-HSA-5678490 ATG16L1 complex transfers LC3 from ATG3 to PE yes reactome/R-HSA-5678490.md ATG16L1 complex transfers LC3 from ATG3 to PE Homo sapiens GO:0019787 ubiquitin-like protein transferase activity TAS enables MF false MODIFY Ubiquitin-like protein transferase activity. Correct in kind but two levels too general; the specific term exists. reaction_mf_refine mf_term_refinement 1 human B3GLCT genes/human/B3GLCT/B3GLCT-ai-review.yaml B3GLCT Q6Y288 NCBITaxon:9606 Homo sapiens 8 R-HSA-5173005 B3GALTL transfers glucose to O-fucosyl-proteins yes reactome/R-HSA-5173005.md B3GALTL transfers glucose to O-fucosyl-proteins Homo sapiens GO:0016757 glycosyltransferase activity TAS enables MF false MODIFY Refine the general activity to the specific reaction described by Reactome. reaction_mf_refine mf_term_refinement 1 human B3GLCT genes/human/B3GLCT/B3GLCT-ai-review.yaml B3GLCT Q6Y288 NCBITaxon:9606 Homo sapiens 9 R-HSA-6785565 Defective B3GALTL does not transfer glucose to O-fucosyl-proteins yes reactome/R-HSA-6785565.md Defective B3GALTL does not transfer glucose to O-fucosyl-proteins Homo sapiens GO:0016757 glycosyltransferase activity TAS enables MF false MODIFY Refine the general wild-type activity while preserving the disease-event provenance. reaction_mf_refine mf_term_refinement reactome_disease_event 1 human BAAT genes/human/BAAT/BAAT-ai-review.yaml BAAT Q14032 NCBITaxon:9606 Homo sapiens 35 R-HSA-159431 Cytosolic chenodeoxycholoyl-CoA or choloyl-CoA are conjugated with glycine or taurine yes reactome/R-HSA-159431.md Cytosolic chenodeoxycholoyl-CoA or choloyl-CoA are conjugated with glycine or taurine Homo sapiens GO:0016746 acyltransferase activity TAS enables MF false MODIFY Reactome (TAS) general acyltransferase activity. Correct but a general parent of the specific bile acid-CoA:amino acid N-acyltransferase function. reaction_mf_refine mf_term_refinement 1 human BAAT genes/human/BAAT/BAAT-ai-review.yaml BAAT Q14032 NCBITaxon:9606 Homo sapiens 36 R-HSA-192312 Choloyl CoA reacts with glycine or taurine to form glycocholate or taurocholate yes reactome/R-HSA-192312.md Choloyl CoA reacts with glycine or taurine to form glycocholate or taurocholate Homo sapiens GO:0016746 acyltransferase activity TAS enables MF false MODIFY Reactome (TAS) general acyltransferase activity (choloyl-CoA + glycine/taurine reaction). Correct but general. reaction_mf_refine mf_term_refinement local_module_mf 1 bile_acid_synthesis_sidechain_conjugation:GO:0047963 glycine N-choloyltransferase activity human BAAT genes/human/BAAT/BAAT-ai-review.yaml BAAT Q14032 NCBITaxon:9606 Homo sapiens 37 R-HSA-193491 Chenodeoxycholoyl CoA reacts with glycine or taurine to form glycochenodeoxycholate or taurochenodeoxycholate yes reactome/R-HSA-193491.md Chenodeoxycholoyl CoA reacts with glycine or taurine to form glycochenodeoxycholate or taurochenodeoxycholate Homo sapiens GO:0016746 acyltransferase activity TAS enables MF false MODIFY Reactome (TAS) general acyltransferase activity (chenodeoxycholoyl-CoA + glycine/taurine reaction). Correct but general. reaction_mf_refine mf_term_refinement 1 human CDK4 genes/human/CDK4/CDK4-ai-review.yaml CDK4 P11802 NCBITaxon:9606 Homo sapiens 233 R-HSA-3215385 CDK4 in CCND1:CDK4:PRMT5:WDR77 phosphorylates WDR77 yes reactome/R-HSA-3215385.md CDK4 in CCND1:CDK4:PRMT5:WDR77 phosphorylates WDR77 Homo sapiens GO:0016538 cyclin-dependent protein serine/threonine kinase regulator activity TAS enables MF false MODIFY Cyclin-dependent protein serine/threonine kinase regulator activity (TAS, Reactome). reaction_mf_refine mf_term_refinement 1 human CDK7 genes/human/CDK7/CDK7-ai-review.yaml CDK7 P50613 NCBITaxon:9606 Homo sapiens 7 R-HSA-6810233 CDK7 phosphorylates serine-5 and serine-7 of heptad repeats in C-terminal domain of RNA polymerase II at snRNA promoter yes reactome/R-HSA-6810233.md CDK7 phosphorylates serine-5 and serine-7 of heptad repeats in C-terminal domain of RNA polymerase II at snRNA promoter Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false MODIFY Cyclin dependence is a conserved characteristic of CDK7. reaction_mf_refine mf_term_refinement 1 human CDK7 genes/human/CDK7/CDK7-ai-review.yaml CDK7 P50613 NCBITaxon:9606 Homo sapiens 8 R-HSA-77071 Phosphorylation (Ser5) of RNA pol II CTD yes reactome/R-HSA-77071.md Phosphorylation (Ser5) of RNA pol II CTD Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false MODIFY Cyclin dependence is a conserved characteristic of CDK7. reaction_mf_refine mf_term_refinement 1 human EHHADH genes/human/EHHADH/EHHADH-ai-review.yaml EHHADH Q08426 NCBITaxon:9606 Homo sapiens 27 R-HSA-6809263 EHHADH hydrates trans-2,3-dehydrohexacosanoyl-CoA yes reactome/R-HSA-6809263.md EHHADH hydrates trans-2,3-dehydrohexacosanoyl-CoA Homo sapiens GO:0018812 3-hydroxyacyl-CoA dehydratase activity TAS enables MF false MODIFY "Reactome labels EHHADH's step-2 hydration reaction as a ""dehydratase"" activity. This is the same reaction captured by the curated enoyl-CoA hydratase activity (GO:0004300), already annotated by IDA. Modify to the curated hydratase term." reaction_mf_refine mf_term_refinement 1 human INPP5D genes/human/INPP5D/INPP5D-ai-review.yaml INPP5D Q92835 NCBITaxon:9606 Homo sapiens 28 R-HSA-202237 Hydrolysis of PIP3 to PI(3,4)P2 yes reactome/R-HSA-202237.md Hydrolysis of PIP3 to PI(3,4)P2 Homo sapiens GO:0016314 phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity TAS enables MF false MODIFY INPP5D hydrolyzes the 5-phosphate of PtdIns(3,4,5)P3, not the 3-phosphate. reaction_mf_refine mf_term_refinement 1 human LPCAT4 genes/human/LPCAT4/LPCAT4-ai-review.yaml LPCAT4 Q643R3 NCBITaxon:9606 Homo sapiens 23 R-HSA-1482539 1-acyl LPG is acylated to PG by LPGAT yes reactome/R-HSA-1482539.md 1-acyl LPG is acylated to PG by LPGAT Homo sapiens GO:0003841 1-acylglycerol-3-phosphate O-acyltransferase activity TAS enables MF false MODIFY The Reactome event supports 1-acyl LPG acylation, but the seeded glycerol-3-phosphate term names the wrong substrate. reaction_mf_refine mf_term_refinement multi_gene_reactome_id 3 human LPCAT4 genes/human/LPCAT4/LPCAT4-ai-review.yaml LPCAT4 Q643R3 NCBITaxon:9606 Homo sapiens 24 R-HSA-1482547 1-acyl LPC is acylated to PC by LPCAT yes reactome/R-HSA-1482547.md 1-acyl LPC is acylated to PC by LPCAT Homo sapiens GO:0003841 1-acylglycerol-3-phosphate O-acyltransferase activity TAS enables MF false MODIFY The Reactome event supports 1-acyl LPC acylation, but the seeded glycerol-3-phosphate term names the wrong substrate. reaction_mf_refine mf_term_refinement multi_gene_reactome_id 4 human LPCAT4 genes/human/LPCAT4/LPCAT4-ai-review.yaml LPCAT4 Q643R3 NCBITaxon:9606 Homo sapiens 25 R-HSA-1482636 1-acyl LPS is acylated to PS by LPSAT yes reactome/R-HSA-1482636.md 1-acyl LPS is acylated to PS by LPSAT Homo sapiens GO:0003841 1-acylglycerol-3-phosphate O-acyltransferase activity TAS enables MF false MODIFY The Reactome event supports 1-acyl LPS acylation, but the seeded glycerol-3-phosphate term names the wrong substrate. reaction_mf_refine mf_term_refinement multi_gene_reactome_id 2 human LPCAT4 genes/human/LPCAT4/LPCAT4-ai-review.yaml LPCAT4 Q643R3 NCBITaxon:9606 Homo sapiens 26 R-HSA-1482667 1-acyl LPE is acylated to PE by LPEAT yes reactome/R-HSA-1482667.md 1-acyl LPE is acylated to PE by LPEAT Homo sapiens GO:0003841 1-acylglycerol-3-phosphate O-acyltransferase activity TAS enables MF false MODIFY The Reactome event supports 1-acyl LPE acylation, but the seeded glycerol-3-phosphate term names the wrong substrate. reaction_mf_refine mf_term_refinement multi_gene_reactome_id 2 human LPCAT4 genes/human/LPCAT4/LPCAT4-ai-review.yaml LPCAT4 Q643R3 NCBITaxon:9606 Homo sapiens 28 R-HSA-1482533 2-acyl LPC is acylated to PC by LPCAT yes reactome/R-HSA-1482533.md 2-acyl LPC is acylated to PC by LPCAT Homo sapiens GO:0047144 2-acylglycerol-3-phosphate O-acyltransferase activity TAS enables MF false MODIFY The Reactome event supports 2-acyl LPC acylation, but the seeded glycerol-3-phosphate term names the wrong substrate. reaction_mf_refine mf_term_refinement multi_gene_reactome_id 4 human LPCAT4 genes/human/LPCAT4/LPCAT4-ai-review.yaml LPCAT4 Q643R3 NCBITaxon:9606 Homo sapiens 30 R-HSA-1482646 2-acyl LPE is acylated to PE by LPEAT yes reactome/R-HSA-1482646.md 2-acyl LPE is acylated to PE by LPEAT Homo sapiens GO:0047144 2-acylglycerol-3-phosphate O-acyltransferase activity TAS enables MF false MODIFY The Reactome event supports 2-acyl LPE acylation, but the seeded glycerol-3-phosphate term names the wrong substrate. reaction_mf_refine mf_term_refinement multi_gene_reactome_id 2 human LPCAT4 genes/human/LPCAT4/LPCAT4-ai-review.yaml LPCAT4 Q643R3 NCBITaxon:9606 Homo sapiens 31 R-HSA-1482691 2-acyl LPS is acylated to PS by LPSAT yes reactome/R-HSA-1482691.md 2-acyl LPS is acylated to PS by LPSAT Homo sapiens GO:0047144 2-acylglycerol-3-phosphate O-acyltransferase activity TAS enables MF false MODIFY The Reactome event supports 2-acyl LPS acylation, but the seeded glycerol-3-phosphate term names the wrong substrate. reaction_mf_refine mf_term_refinement multi_gene_reactome_id 2 human LPGAT1 genes/human/LPGAT1/LPGAT1-ai-review.yaml LPGAT1 Q92604 NCBITaxon:9606 Homo sapiens 15 R-HSA-1482539 1-acyl LPG is acylated to PG by LPGAT yes reactome/R-HSA-1482539.md 1-acyl LPG is acylated to PG by LPGAT Homo sapiens GO:0003841 1-acylglycerol-3-phosphate O-acyltransferase activity TAS enables MF false MODIFY The cited remodeling event concerns lysophosphatidylglycerol or other lysophospholipid acylation, not the named lysophosphatidic-acid reaction. reaction_mf_refine mf_term_refinement multi_gene_reactome_id 3 human LPGAT1 genes/human/LPGAT1/LPGAT1-ai-review.yaml LPGAT1 Q92604 NCBITaxon:9606 Homo sapiens 16 R-HSA-1482635 2-acyl LPG is acylated to PG by LPGAT yes reactome/R-HSA-1482635.md 2-acyl LPG is acylated to PG by LPGAT Homo sapiens GO:0047144 2-acylglycerol-3-phosphate O-acyltransferase activity TAS enables MF false MODIFY The cited remodeling event concerns lysophosphatidylglycerol or other lysophospholipid acylation, not the named lysophosphatidic-acid reaction. reaction_mf_refine mf_term_refinement multi_gene_reactome_id 3 human LRP2 genes/human/LRP2/LRP2-ai-review.yaml LRP2 P98164 NCBITaxon:9606 Homo sapiens 59 R-HSA-2404131 LRPs transport extracellular CR:atREs:HSPG:apoE to cytosol yes reactome/R-HSA-2404131.md LRPs transport extracellular CR:atREs:HSPG:apoE to cytosol Homo sapiens GO:0005041 low-density lipoprotein particle receptor activity TAS enables MF false MODIFY Reactome:R-HSA-2404131 provides statement-level support for the LRP2 annotation to low-density lipoprotein particle receptor activity. reaction_mf_refine mf_term_refinement multi_gene_reactome_id 3 human MAP2K2 genes/human/MAP2K2/MAP2K2-ai-review.yaml MAP2K2 P36507 NCBITaxon:9606 Homo sapiens 3 R-HSA-109862 MAP2K2 phosphorylates MAPK1 yes reactome/R-HSA-109862.md MAP2K2 phosphorylates MAPK1 Homo sapiens GO:0004674 protein serine/threonine kinase activity TAS enables MF false MODIFY MEK2 phosphorylates both threonine and tyrosine in ERK activation loops; MAP kinase kinase activity is the informative function. reaction_mf_refine mf_term_refinement 1 human MGAT1 genes/human/MGAT1/MGAT1-ai-review.yaml MGAT1 P26572 NCBITaxon:9606 Homo sapiens 14 R-HSA-9683648 Spike trimer glycoside chains are extended yes reactome/R-HSA-9683648.md Spike trimer glycoside chains are extended Homo sapiens GO:0008375 acetylglucosaminyltransferase activity TAS enables MF false MODIFY "Reactome MF annotation from ""Spike trimer glycoside chains are extended"", which states GlcNAc-TI (MGAT1) adds a GlcNAc residue to high-mannose chains. Correct direction but over-general; the specific GlcNAc-TI term GO:0003827 captures the activity." reaction_mf_refine mf_term_refinement 1 human MGAT1 genes/human/MGAT1/MGAT1-ai-review.yaml MGAT1 P26572 NCBITaxon:9606 Homo sapiens 15 R-HSA-9694656 Spike trimer glycoside chains are extended yes reactome/R-HSA-9694656.md Spike trimer glycoside chains are extended Homo sapiens GO:0008375 acetylglucosaminyltransferase activity TAS enables MF false MODIFY "Duplicate Reactome ""Spike trimer glycoside chains are extended"" MF annotation. Same over-general issue; replace with the specific GlcNAc-TI activity term." reaction_mf_refine mf_term_refinement 1 human PIGA genes/human/PIGA/PIGA-ai-review.yaml PIGA P37287 NCBITaxon:9606 Homo sapiens 18 R-HSA-162730 phosphatidylinositol + UDP-N-acetyl-D-glucosamine -> N-acetylglucosaminyl-PI + UDP yes reactome/R-HSA-162730.md phosphatidylinositol + UDP-N-acetyl-D-glucosamine -> N-acetylglucosaminyl-PI + UDP Homo sapiens GO:0008194 UDP-glycosyltransferase activity TAS enables MF false MODIFY Reactome describes the reaction as using the UDP-sugar donor UDP-GlcNAc. The term correctly captures the UDP-sugar donor class but is less specific than the exact enzyme activity. reaction_mf_refine mf_term_refinement local_module_mf 1 gpi_anchor_glcnac_transferase:GO:0017176 phosphatidylinositol N-acetylglucosaminyltransferase activity human PIP5K1B genes/human/PIP5K1B/PIP5K1B-ai-review.yaml PIP5K1B O14986 NCBITaxon:9606 Homo sapiens 14 R-HSA-1675773 PI(3,4)P2 is phosphorylated to PI(3,4,5)P3 by PIP5K1A-C at the plasma membrane yes reactome/R-HSA-1675773.md PI(3,4)P2 is phosphorylated to PI(3,4,5)P3 by PIP5K1A-C at the plasma membrane Homo sapiens GO:0052742 phosphatidylinositol kinase activity TAS enables MF false MODIFY Phosphatidylinositol kinase activity is true but too general for PIP5K1B, whose defining activity is PI4P 5-kinase activity. reaction_mf_refine mf_term_refinement 1 human PLCG2 genes/human/PLCG2/PLCG2-ai-review.yaml PLCG2 P16885 NCBITaxon:9606 Homo sapiens 58 R-HSA-1112666 BLNK (SLP-65) Signalosome hydrolyzes phosphatidyinositol bisphosphate forming diacylglycerol and inositol-1,4,5-trisphosphate yes reactome/R-HSA-1112666.md BLNK (SLP-65) Signalosome hydrolyzes phosphatidyinositol bisphosphate forming diacylglycerol and inositol-1,4,5-trisphosphate Homo sapiens GO:0004629 C-type glycerophospholipase activity TAS enables MF false MODIFY The annotation is directionally correct but too broad for PLCG2; the specific biology is PIP2 phospholipase activity and phosphoinositide-mediated receptor signaling. reaction_mf_refine mf_term_refinement 1 human PLCG2 genes/human/PLCG2/PLCG2-ai-review.yaml PLCG2 P16885 NCBITaxon:9606 Homo sapiens 59 R-HSA-114689 PLC gamma 2-mediated PIP2 hydrolysis yes reactome/R-HSA-114689.md PLC gamma 2-mediated PIP2 hydrolysis Homo sapiens GO:0004629 C-type glycerophospholipase activity TAS enables MF false MODIFY The annotation is directionally correct but too broad for PLCG2; the specific biology is PIP2 phospholipase activity and phosphoinositide-mediated receptor signaling. reaction_mf_refine mf_term_refinement 1 human PNPLA3 genes/human/PNPLA3/PNPLA3-ai-review.yaml PNPLA3 Q9NST1 NCBITaxon:9606 Homo sapiens 26 R-HSA-1482811 DAG is hydrolyzed to 2-MAG by PNPLA2/3 yes reactome/R-HSA-1482811.md DAG is hydrolyzed to 2-MAG by PNPLA2/3 Homo sapiens GO:0004465 lipoprotein lipase activity TAS enables MF false MODIFY Lipoprotein lipase activity asserted from the Reactome reaction 'DAG is hydrolyzed to 2-MAG by PNPLA2/3'. reaction_mf_refine mf_term_refinement 1 human PNPLA3 genes/human/PNPLA3/PNPLA3-ai-review.yaml PNPLA3 Q9NST1 NCBITaxon:9606 Homo sapiens 31 R-HSA-1482647 2-MAG and DAG are transacylated to TAG by PNPLA2/3 yes reactome/R-HSA-1482647.md 2-MAG and DAG are transacylated to TAG by PNPLA2/3 Homo sapiens GO:0016411 acylglycerol O-acyltransferase activity TAS enables MF false MODIFY Acyl transfer onto an acylglycerol, from the Reactome transacylation reactions. reaction_mf_refine mf_term_refinement 1 human PNPLA3 genes/human/PNPLA3/PNPLA3-ai-review.yaml PNPLA3 Q9NST1 NCBITaxon:9606 Homo sapiens 32 R-HSA-1482654 2-MAG is transacylated to DAG by PNPLA2/3 yes reactome/R-HSA-1482654.md 2-MAG is transacylated to DAG by PNPLA2/3 Homo sapiens GO:0016411 acylglycerol O-acyltransferase activity TAS enables MF false MODIFY Acyl transfer onto an acylglycerol, from the Reactome transacylation reactions. reaction_mf_refine mf_term_refinement 1 human PSMB5 genes/human/PSMB5/PSMB5-ai-review.yaml PSMB5 P28074 NCBITaxon:9606 Homo sapiens 66 R-HSA-9912636 Proteasomal cleavage of intracellular substrate (PA28-alpha-beta-20S proteasome catalyst) yes reactome/R-HSA-9912636.md Proteasomal cleavage of intracellular substrate (PA28-alpha-beta-20S proteasome catalyst) Homo sapiens GO:0004175 endopeptidase activity TAS enables MF false MODIFY endopeptidase activity is directionally related to the proteasome role but is less specific than the supported proteasome term. reaction_mf_refine mf_term_refinement 1 human PSMB5 genes/human/PSMB5/PSMB5-ai-review.yaml PSMB5 P28074 NCBITaxon:9606 Homo sapiens 67 R-HSA-9908101 Maturation of the canonical 20S core particle yes reactome/R-HSA-9908101.md Maturation of the canonical 20S core particle Homo sapiens GO:0004175 endopeptidase activity TAS enables MF false MODIFY endopeptidase activity is directionally related to the proteasome role but is less specific than the supported proteasome term. reaction_mf_refine mf_term_refinement 1 human RCE1 genes/human/RCE1/RCE1-ai-review.yaml RCE1 Q9Y256 NCBITaxon:9606 Homo sapiens 10 R-HSA-9647999 RCE1 cleaves S-Farn proRAS proteins yes reactome/R-HSA-9647999.md RCE1 cleaves S-Farn proRAS proteins Homo sapiens GO:0004197 cysteine-type endopeptidase activity TAS enables MF false MODIFY RCE1 is a glutamate-type protease, not a cysteine-type endopeptidase; wrong catalytic class. reaction_mf_refine mf_term_refinement 1 human RDH11 genes/human/RDH11/RDH11-ai-review.yaml RDH11 Q8TC12 NCBITaxon:9606 Homo sapiens 14 R-HSA-5362721 RDH5,RDH11 oxidise 11cROL to 11cRAL yes reactome/R-HSA-5362721.md RDH5,RDH11 oxidise 11cROL to 11cRAL Homo sapiens GO:0004745 all-trans-retinol dehydrogenase (NAD+) activity TAS enables MF false MODIFY NAD+-dependent all-trans-retinol dehydrogenase, Reactome TAS. RDH11 has a clear preference for NADP(H) (~800-fold lower Km for NADP+/NADPH than NAD+/NADH), so the NAD+-specific cofactor term misrepresents the physiological cofactor. The correct cofactor-specific term is the NADP+ form (GO:0052650). reaction_mf_refine mf_term_refinement multi_gene_reactome_id 2 human RDH5 genes/human/RDH5/RDH5-ai-review.yaml RDH5 Q92781 NCBITaxon:9606 Homo sapiens 17 R-HSA-2454081 RDH5 oxidises 11cROL to 11cRAL yes reactome/R-HSA-2454081.md RDH5 oxidises 11cROL to 11cRAL Homo sapiens GO:0004745 all-trans-retinol dehydrogenase (NAD+) activity TAS enables MF false MODIFY "Reactome maps RDH5 catalysis to the generic all-trans-retinol dehydrogenase GO term, but the Reactome reaction it annotates (""RDH5 oxidises 11cROL to 11cRAL"") is explicitly the 11-cis reaction, and UniProt shows RDH5 has no all-trans activity. Modify to the cis-specific term." reaction_mf_refine mf_term_refinement 1 human RDH5 genes/human/RDH5/RDH5-ai-review.yaml RDH5 Q92781 NCBITaxon:9606 Homo sapiens 18 R-HSA-2466832 Defective RDH5 does not oxidise 11cROL to 11cRAL and causes RPA yes reactome/R-HSA-2466832.md Defective RDH5 does not oxidise 11cROL to 11cRAL and causes RPA Homo sapiens GO:0004745 all-trans-retinol dehydrogenase (NAD+) activity TAS enables MF false MODIFY "Reactome reaction (""Defective RDH5 does not oxidise 11cROL to 11cRAL"") again describes the 11-cis reaction but is mapped to the generic all-trans GO term. Same wrong-substrate issue; modify to the 11-cis-specific molecular function." reaction_mf_refine mf_term_refinement reactome_disease_event 1 human RDH5 genes/human/RDH5/RDH5-ai-review.yaml RDH5 Q92781 NCBITaxon:9606 Homo sapiens 19 R-HSA-5362721 RDH5,RDH11 oxidise 11cROL to 11cRAL yes reactome/R-HSA-5362721.md RDH5,RDH11 oxidise 11cROL to 11cRAL Homo sapiens GO:0004745 all-trans-retinol dehydrogenase (NAD+) activity TAS enables MF false MODIFY "Reactome reaction (""RDH5,RDH11 oxidise 11cROL to 11cRAL"") is the 11-cis oxidation mapped to the generic all-trans GO term. Modify to the substrate-correct 11-cis term." reaction_mf_refine mf_term_refinement multi_gene_reactome_id 2 human SHH genes/human/SHH/SHH-ai-review.yaml SHH Q15465 NCBITaxon:9606 Homo sapiens 84 R-HSA-5358460 HPE SHH variants don't undergo autoproteolytic cleavage yes reactome/R-HSA-5358460.md HPE SHH variants don't undergo autoproteolytic cleavage Homo sapiens GO:0004175 endopeptidase activity TAS enables MF false MODIFY SHH cleavage is coupled to cholesterol transfer; the precise current activity term is cholesterol-protein transferase activity. This GOA record uses traceable-author-statement evidence (TAS) from Reactome:R-HSA-5358460. reaction_mf_refine mf_term_refinement 1 human SLC8B1 genes/human/SLC8B1/SLC8B1-ai-review.yaml SLC8B1 Q6J4K2 NCBITaxon:9606 Homo sapiens 31 R-HSA-8949688 SLC8B1 (NCLX) exchanges sodium (mitochondrial intermembrane space) for calcium (mitochondrial matrix) yes reactome/R-HSA-8949688.md SLC8B1 (NCLX) exchanges sodium (mitochondrial intermembrane space) for calcium (mitochondrial matrix) Homo sapiens GO:0005432 calcium:sodium antiporter activity TAS enables MF false MODIFY Calcium:sodium antiport, the classical assignment for NCLX. reaction_mf_refine mf_term_refinement 1 human ST3GAL5 genes/human/ST3GAL5/ST3GAL5-ai-review.yaml ST3GAL5 Q9UNP4 NCBITaxon:9606 Homo sapiens 8 R-HSA-4084984 ST3GAL1-6 transfer Neu5Ac to terminal Gal (alpha-2,3 link) yes reactome/R-HSA-4084984.md ST3GAL1-6 transfer Neu5Ac to terminal Gal (alpha-2,3 link) Homo sapiens GO:0003836 beta-galactoside (CMP) alpha-2,3-sialyltransferase activity TAS enables MF false MODIFY "Reactome (TAS) family-level annotation from the reaction ""ST3GAL1-6 transfer Neu5Ac to terminal Gal (alpha-2,3 link)"". GO:0003836 is defined for the beta-D-galactosyl-(1->3)-GalNAc-R (O-glycan) acceptor reaction shared by the ST3GAL family, not for the LacCer -> GM3 reaction that defines ST3GAL5. The gene-specific term GO:0047291 better represents this enzyme's characterized activity." reaction_mf_refine mf_term_refinement 1 human ST3GAL5 genes/human/ST3GAL5/ST3GAL5-ai-review.yaml ST3GAL5 Q9UNP4 NCBITaxon:9606 Homo sapiens 9 R-HSA-9844860 ST3GAL5 transfers NeuNAc to ceramides yes reactome/R-HSA-9844860.md ST3GAL5 transfers NeuNAc to ceramides Homo sapiens GO:0003836 beta-galactoside (CMP) alpha-2,3-sialyltransferase activity TAS enables MF false MODIFY "Reactome (TAS) annotation from ""ST3GAL5 transfers NeuNAc to ceramides"". Although the Reactome reaction is ST3GAL5-specific, the mapped GO term GO:0003836 is the generic beta-galactoside (O-glycan Gal-1,3-GalNAc-R) sialyltransferase term rather than the glycosphingolipid GM3 synthase term. The gene-specific GO:0047291 more precisely captures the LacCer -> GM3 activity described by this Reactome event." reaction_mf_refine mf_term_refinement 1 human ST6GAL1 genes/human/ST6GAL1/ST6GAL1-ai-review.yaml ST6GAL1 P15907 NCBITaxon:9606 Homo sapiens 21 R-HSA-9683769 O-glycosylation of 3a is terminated yes reactome/R-HSA-9683769.md O-glycosylation of 3a is terminated Homo sapiens GO:0008373 sialyltransferase activity TAS enables MF false MODIFY Over-general parent MF asserted within a SARS-CoV-2 O-glycosylation Reactome reaction. The specific activity GO:0003835 is established for ST6GAL1 and should be used instead of the parent sialyltransferase activity term. reaction_mf_refine mf_term_refinement 1 human ST6GAL1 genes/human/ST6GAL1/ST6GAL1-ai-review.yaml ST6GAL1 P15907 NCBITaxon:9606 Homo sapiens 22 R-HSA-9697018 Addition of sialic acids on some Spike glycosyl sidechains yes reactome/R-HSA-9697018.md Addition of sialic acids on some Spike glycosyl sidechains Homo sapiens GO:0008373 sialyltransferase activity TAS enables MF false MODIFY Over-general parent MF asserted within a SARS-CoV-2 spike sialylation Reactome reaction. Replace with the specific GO:0003835 established for ST6GAL1. reaction_mf_refine mf_term_refinement 1 human STAR genes/human/STAR/STAR-ai-review.yaml STAR P49675 NCBITaxon:9606 Homo sapiens 35 R-HSA-196126 STAR mediates CHOL translocation from cytosol to mitochondrial intermembrane space yes reactome/R-HSA-196126.md STAR mediates CHOL translocation from cytosol to mitochondrial intermembrane space Homo sapiens GO:0140104 molecular carrier activity TAS enables MF false MODIFY Reactome traceable annotation to the broad molecular carrier activity term. StAR is indeed a cholesterol carrier, but the specific term cholesterol transfer activity (GO:0120020) is far more informative and is well supported experimentally. reaction_mf_refine mf_term_refinement local_module_mf 1 adrenal_steroidogenesis:GO:0120020 cholesterol transfer activity human TECR genes/human/TECR/TECR-ai-review.yaml TECR Q9NZ01 NCBITaxon:9606 Homo sapiens 17 R-HSA-548831 TECR,TECRL dehydrogenate TOD-CoA to ST-CoA yes reactome/R-HSA-548831.md TECR,TECRL dehydrogenate TOD-CoA to ST-CoA Homo sapiens GO:0017099 very-long-chain fatty acyl-CoA dehydrogenase activity TAS enables MF false MODIFY "Reactome models the TECR-catalyzed reaction (trans-octadec-2-enoyl-CoA + NADPH -> stearoyl-CoA + NADP) in the reverse ""dehydrogenate"" direction and maps it to a dehydrogenase molecular function. Physiologically TECR is a reductase (NADPH-consuming), catalyzing the fourth reductive step of elongation; the dehydrogenase MF misrepresents the enzyme's biological direction. Modify to the correct reductase MF (GO:0102758). Not removed (TAS; same catalytic activity written in the reverse direction)." reaction_mf_refine mf_term_refinement 1 human TRAF3 genes/human/TRAF3/TRAF3-ai-review.yaml TRAF3 Q13114 NCBITaxon:9606 Homo sapiens 69 R-HSA-2213017 Auto-ubiquitination of TRAF3 yes reactome/R-HSA-2213017.md Auto-ubiquitination of TRAF3 Homo sapiens GO:0004842 ubiquitin-protein transferase activity TAS enables MF false MODIFY Parent term for ubiquitin transfer; TRAF3's activity is specifically that of a RING-type E3 ubiquitin ligase. reaction_mf_refine mf_term_refinement 1 human TRAF3 genes/human/TRAF3/TRAF3-ai-review.yaml TRAF3 Q13114 NCBITaxon:9606 Homo sapiens 70 R-HSA-9013974 Auto-ubiquitination of TRAF3 within activated TLR3 complex yes reactome/R-HSA-9013974.md Auto-ubiquitination of TRAF3 within activated TLR3 complex Homo sapiens GO:0004842 ubiquitin-protein transferase activity TAS enables MF false MODIFY Parent term for ubiquitin transfer; TRAF3's activity is specifically that of a RING-type E3 ubiquitin ligase. reaction_mf_refine mf_term_refinement 1 human SRD5A3 genes/human/SRD5A3/SRD5A3-ai-review.yaml SRD5A3 Q9H8P0 NCBITaxon:9606 Homo sapiens 14 R-HSA-9705714 SRD5A3 dehydrogenates TEST to DHTEST yes reactome/R-HSA-9705714.md SRD5A3 dehydrogenates TEST to DHTEST Homo sapiens GO:0003865 3-oxo-5-alpha-steroid 4-dehydrogenase activity TAS enables MF false MARK_AS_OVER_ANNOTATED Reactome-derived steroid 5-alpha-reductase MF annotation (SRD5A3 dehydrogenates TEST to DHTEST), tracing to the in-vitro testosterone-to-DHT activity of Uemura et al. 2008. This is a weak, non-physiological side activity and an over-annotation relative to the enzyme's dolichol-pathway function. nonphysiological_side_activity nonphysiological_side_activity nonphysiological_side_activity nonphysiological_side_activity The testosterone-to-DHT event reflects a weak in-vitro side activity rather than SRD5A3's physiological dolichol-pathway function. manual_verdict 1 human CFTR genes/human/CFTR/CFTR-ai-review.yaml CFTR P13569 NCBITaxon:9606 Homo sapiens 107 R-HSA-1454916 The ABCC family mediates organic anion transport yes reactome/R-HSA-1454916.md The ABCC family mediates organic anion transport Homo sapiens GO:0043225 obsolete ATPase-coupled inorganic anion transmembrane transporter activity TAS enables MF false REMOVE This annotation uses an obsolete GO term and should be replaced with current terminology. obsolete_go_term obsolete_go_term obsolete_go_term obsolete_go_term The row uses an obsolete ATPase-coupled inorganic anion transporter term. manual_verdict|multi_gene_reactome_id 3 human RASA1 genes/human/RASA1/RASA1-ai-review.yaml RASA1 P20936 NCBITaxon:9606 Homo sapiens 33 R-HSA-8981353 RASA1 stimulates RAS GTPase activity yes reactome/R-HSA-8981353.md RASA1 stimulates RAS GTPase activity Homo sapiens GO:0003924 GTPase activity TAS enables MF false REMOVE Reactome annotation indicating RASA1 involvement in GTPase reactions. However, RASA1 itself does not have intrinsic GTPase activity - it stimulates the GTPase activity of Ras proteins. regulator_not_executor regulator_not_executor regulator_not_executor regulator_not_executor RASA1 stimulates Ras-family GTPases but is not itself the GTP-hydrolyzing enzyme in the event. manual_verdict 1 human RGS20 genes/human/RGS20/RGS20-ai-review.yaml RGS20 O76081 NCBITaxon:9606 Homo sapiens 19 R-HSA-8982020 G alpha (i)-i1/i2/i3 in G (i):RGS complex is inactivated yes reactome/R-HSA-8982020.md G alpha (i)–i1/i2/i3 in G (i):RGS complex is inactivated Homo sapiens GO:0003924 GTPase activity TAS enables MF false REMOVE This Reactome annotation incorrectly assigns GTPase activity to RGS20. RGS20 does not hydrolyze GTP itself; it accelerates GTP hydrolysis by Galpha subunits. regulator_not_executor regulator_not_executor regulator_not_executor regulator_not_executor RGS20 accelerates GTP hydrolysis by G alpha(i) subunits but does not hydrolyze GTP itself. manual_verdict 1 human RGS20 genes/human/RGS20/RGS20-ai-review.yaml RGS20 O76081 NCBITaxon:9606 Homo sapiens 20 R-HSA-8982021 G alpha (z) in G alpha (z):RGS complex is inactivated yes reactome/R-HSA-8982021.md G alpha (z) in G alpha (z):RGS complex is inactivated Homo sapiens GO:0003924 GTPase activity TAS enables MF false REMOVE Same issue as above - incorrect assignment of GTPase activity to RGS20. regulator_not_executor regulator_not_executor regulator_not_executor regulator_not_executor RGS20 accelerates GTP hydrolysis by G alpha(z) but does not hydrolyze GTP itself. manual_verdict 1 human ATF2 genes/human/ATF2/ATF2-ai-review.yaml ATF2 P15336 NCBITaxon:9606 Homo sapiens 78 R-HSA-3318415 ATF2 acetylates histone H2B, H4 yes reactome/R-HSA-3318415.md ATF2 acetylates histone H2B, H4 Homo sapiens GO:0004402 histone acetyltransferase activity TAS enables MF false UNDECIDED ATF2 has reported intrinsic HAT activity toward H2B and H4 (PMID:10821277, Kawasaki et al. 2000, Nature). However, UniProt notes a CAUTION that this activity may be due to contamination. Reactome pathway R-HSA-3318415 describes ATF2 acetylating H2B and H4. insufficient_event_detail undecided_mf 1 human LPCAT3 genes/human/LPCAT3/LPCAT3-ai-review.yaml LPCAT3 Q6P1A2 NCBITaxon:9606 Homo sapiens 16 R-HSA-1482547 1-acyl LPC is acylated to PC by LPCAT yes reactome/R-HSA-1482547.md 1-acyl LPC is acylated to PC by LPCAT Homo sapiens GO:0003841 1-acylglycerol-3-phosphate O-acyltransferase activity TAS enables MF false UNDECIDED Reactome annotation for AGPAT-like activity. However, LPCAT3 acts on lysophospholipids (with headgroups like choline, ethanolamine, serine), not on lysophosphatidic acid (LPA). This activity is distinct from true AGPAT/LPAAT activity. insufficient_event_detail undecided_mf multi_gene_reactome_id 4 human LPCAT3 genes/human/LPCAT3/LPCAT3-ai-review.yaml LPCAT3 Q6P1A2 NCBITaxon:9606 Homo sapiens 17 R-HSA-1482636 1-acyl LPS is acylated to PS by LPSAT yes reactome/R-HSA-1482636.md 1-acyl LPS is acylated to PS by LPSAT Homo sapiens GO:0003841 1-acylglycerol-3-phosphate O-acyltransferase activity TAS enables MF false UNDECIDED Second Reactome annotation for AGPAT activity. insufficient_event_detail undecided_mf multi_gene_reactome_id 2 human LPCAT3 genes/human/LPCAT3/LPCAT3-ai-review.yaml LPCAT3 Q6P1A2 NCBITaxon:9606 Homo sapiens 18 R-HSA-1482667 1-acyl LPE is acylated to PE by LPEAT yes reactome/R-HSA-1482667.md 1-acyl LPE is acylated to PE by LPEAT Homo sapiens GO:0003841 1-acylglycerol-3-phosphate O-acyltransferase activity TAS enables MF false UNDECIDED Third Reactome annotation for AGPAT activity. insufficient_event_detail undecided_mf multi_gene_reactome_id 2 human LPCAT3 genes/human/LPCAT3/LPCAT3-ai-review.yaml LPCAT3 Q6P1A2 NCBITaxon:9606 Homo sapiens 19 R-HSA-1482533 2-acyl LPC is acylated to PC by LPCAT yes reactome/R-HSA-1482533.md 2-acyl LPC is acylated to PC by LPCAT Homo sapiens GO:0047144 2-acylglycerol-3-phosphate O-acyltransferase activity TAS enables MF false UNDECIDED Reactome annotation for acyltransferase activity at the sn-1 position of 2-acyl-lysophospholipids. insufficient_event_detail undecided_mf multi_gene_reactome_id 4 human LPCAT3 genes/human/LPCAT3/LPCAT3-ai-review.yaml LPCAT3 Q6P1A2 NCBITaxon:9606 Homo sapiens 20 R-HSA-1482646 2-acyl LPE is acylated to PE by LPEAT yes reactome/R-HSA-1482646.md 2-acyl LPE is acylated to PE by LPEAT Homo sapiens GO:0047144 2-acylglycerol-3-phosphate O-acyltransferase activity TAS enables MF false UNDECIDED Second Reactome annotation for 2-AGPAT activity. insufficient_event_detail undecided_mf multi_gene_reactome_id 2 human LPCAT3 genes/human/LPCAT3/LPCAT3-ai-review.yaml LPCAT3 Q6P1A2 NCBITaxon:9606 Homo sapiens 21 R-HSA-1482691 2-acyl LPS is acylated to PS by LPSAT yes reactome/R-HSA-1482691.md 2-acyl LPS is acylated to PS by LPSAT Homo sapiens GO:0047144 2-acylglycerol-3-phosphate O-acyltransferase activity TAS enables MF false UNDECIDED Third Reactome annotation for 2-AGPAT activity. insufficient_event_detail undecided_mf multi_gene_reactome_id 2 human MMAA genes/human/MMAA/MMAA-ai-review.yaml MMAA Q8IVH4 NCBITaxon:9606 Homo sapiens 13 R-HSA-3159259 MMAA:MUT binds AdoCbl no GO:0140104 molecular carrier activity TAS enables MF false MARK_AS_OVER_ANNOTATED "Reactome TAS (""MMAA:MUT binds AdoCbl"") assigning molecular carrier activity. MMAA does not itself carry cobalamin as cargo; it is the G-protein that gates the transfer of AdoCbl (produced by MMAB) onto MMUT in a GTP-dependent manner. ""Molecular carrier activity"" mislabels the gating/regulatory GTPase role as direct cofactor transport." wrong_executor wrong_executor wrong_executor wrong_executor MMAA is the GTPase that gates cobalamin transfer onto MMUT, not the molecular carrier of cobalamin cargo. manual_verdict|local_module_mf|uncached_reactome 1 propionyl_coa_catabolism:GO:0003924 GTPase activity human TXN genes/human/TXN/TXN-ai-review.yaml TXN P10599 NCBITaxon:9606 Homo sapiens 28 R-NUL-9617742 Reactome pathway annotation no GO:0004791 thioredoxin-disulfide reductase (NADPH) activity TAS enables MF false REMOVE Reactome annotation. Note that this GO term describes the activity of thioredoxin reductase, not thioredoxin itself. wrong_executor wrong_executor wrong_executor wrong_executor The thioredoxin-disulfide reductase MF belongs to thioredoxin reductase rather than thioredoxin. manual_verdict|uncached_reactome 1 human ASMT genes/human/ASMT/ASMT-ai-review.yaml ASMT P46597 NCBITaxon:9606 Homo sapiens 11 R-HSA-209821 Methylation of N-acetyl-5-HT to form melatonin yes reactome/R-HSA-209821.md Methylation of N-acetyl-5-HT to form melatonin Homo sapiens GO:0008172 S-methyltransferase activity TAS enables MF false MARK_AS_OVER_ANNOTATED TAS (Reactome) annotation to S-methyltransferase activity. This is the WRONG MOLECULAR-FUNCTION BRANCH: S-methyltransferases transfer a methyl group to a sulfur atom, whereas ASMT is definitively an O-methyltransferase (EC 2.1.1.4) that methylates the 5-hydroxyl oxygen of N-acetylserotonin. This is a Reactome-to-GO mapping artifact (the reaction consumes SAM, but the acceptor atom is oxygen, not sulfur). Not experimental. Mark as over-annotated; the correct MF is GO:0017096 / GO:0008171. wrong_go_branch wrong_go_branch wrong_go_branch wrong_go_branch ASMT methylates the 5-hydroxyl oxygen of N-acetylserotonin, so the Reactome event was mapped to the sulfur-acceptor methyltransferase branch by mistake. manual_verdict 1 human ATP13A1 genes/human/ATP13A1/ATP13A1-ai-review.yaml ATP13A1 Q9HD20 NCBITaxon:9606 Homo sapiens 21 R-HSA-5692462 ATP13A1 transports Mn2+ from cytosol to ER lumen yes reactome/R-HSA-5692462.md ATP13A1 transports Mn2+ from cytosol to ER lumen Homo sapiens GO:0015410 ABC-type manganese transporter activity TAS enables MF false REMOVE ATP13A1 is a P-type ATPase, not an ABC-type manganese transporter. wrong_go_branch wrong_go_branch wrong_go_branch wrong_go_branch ATP13A1 is a P-type ATPase, not an ABC-type manganese transporter. manual_verdict 1 human AP4M1 genes/human/AP4M1/AP4M1-ai-review.yaml AP4M1 O00189 NCBITaxon:9606 Homo sapiens 33 R-HSA-5229111 AP4 transports APP from trans-Golgi network to endosome lumen yes reactome/R-HSA-5229111.md AP4 transports APP from trans-Golgi network to endosome lumen Homo sapiens GO:0008320 transmembrane protein transporter activity TAS enables MF false REMOVE Asserts that AP4M1 moves a protein from one side of a membrane to the other. It does not. AP-4 selects cargo into vesicles; nothing crosses a bilayer. wrong_transport_mechanism wrong_transport_mechanism wrong_transport_mechanism wrong_transport_mechanism AP-4 selects APP into vesicles but does not move APP from one side of a membrane to the other. manual_verdict 1