- organism: human gene: ASMT reactome_id: R-HSA-209821 term_id: GO:0008172 manual_verdict: wrong_go_branch manual_bucket: wrong_go_branch manual_reason: "ASMT methylates the 5-hydroxyl oxygen of N-acetylserotonin, so the Reactome event was mapped to the sulfur-acceptor methyltransferase branch by mistake." - organism: human gene: B4GALT5 reactome_id: R-HSA-1964501 term_id: GO:0008378 manual_verdict: reaction_mf_refine manual_bucket: broad_parent_or_duplicate manual_reason: "Reactome supports a galactosyltransferase reaction, but the row uses a generic parent that is less informative than LacCer synthase or beta-1,4-galactosyltransferase terms." - organism: human gene: CYP19A1 reactome_id: R-HSA-193060 term_id: GO:0008395 manual_verdict: reaction_mf_refine manual_bucket: broad_parent_or_duplicate manual_reason: "The steroid hydroxylase parent is mechanistically true for the first C19 hydroxylation step but is over-general relative to aromatase activity." - organism: human gene: CYP19A1 reactome_id: R-HSA-193143 term_id: GO:0008395 manual_verdict: reaction_mf_refine manual_bucket: broad_parent_or_duplicate manual_reason: "The steroid hydroxylase parent is a duplicate broad parent for the testosterone-to-estradiol Reactome reaction." - organism: human gene: CYP19A1 reactome_id: R-HSA-5601849 term_id: GO:0008395 manual_verdict: reaction_mf_refine manual_bucket: broad_parent_or_duplicate manual_reason: "The disease event restates the same broad steroid hydroxylase parent instead of the specific aromatase MF." - organism: human gene: CYP51A1 reactome_id: R-HSA-194678 term_id: GO:0016712 manual_verdict: reaction_mf_refine manual_bucket: broad_parent_or_duplicate manual_reason: "The P450 monooxygenase parent is mechanistically sound but less specific than sterol 14-demethylase activity." - organism: human gene: DBT reactome_id: R-HSA-9865115 term_id: GO:0016747 manual_verdict: reaction_mf_refine manual_bucket: broad_parent_or_duplicate manual_reason: "The disease event supports DBT acyltransferase chemistry only at a broad parent level." - organism: human gene: DBT reactome_id: R-HSA-9859163 term_id: GO:0016747 manual_verdict: reaction_mf_refine manual_bucket: broad_parent_or_duplicate manual_reason: "The normal BCAA-to-CoA event supports DBT acyltransferase chemistry only at a broad parent level." - organism: human gene: FAR2 reactome_id: R-HSA-390438 term_id: GO:0016491 manual_verdict: reaction_mf_refine manual_bucket: broad_parent_or_duplicate manual_reason: "The oxidoreductase parent is chemically correct but much broader than fatty acyl-CoA reductase activity." - organism: human gene: GAMT reactome_id: R-HSA-71286 term_id: GO:0008168 manual_verdict: reaction_mf_refine manual_bucket: broad_parent_or_duplicate manual_reason: "The methyltransferase parent is correct but broader than guanidinoacetate N-methyltransferase activity." - organism: human gene: LRAT reactome_id: R-HSA-2404137 term_id: GO:0016416 manual_verdict: reaction_mf_refine manual_bucket: broad_parent_or_duplicate manual_reason: "The palmitoyltransferase row captures one LRAT acyl group but is a less informative sibling of phosphatidylcholine-retinol O-acyltransferase." - organism: human gene: LRAT reactome_id: R-HSA-2453855 term_id: GO:0016416 manual_verdict: reaction_mf_refine manual_bucket: broad_parent_or_duplicate manual_reason: "This duplicates the same broad palmitoyltransferase mapping for LRAT." - organism: human gene: LRAT reactome_id: R-HSA-2466710 term_id: GO:0016416 manual_verdict: reaction_mf_refine manual_bucket: broad_parent_or_duplicate manual_reason: "The disease event repeats the same broad palmitoyltransferase mapping for LRAT." - organism: human gene: MAGT1 reactome_id: R-HSA-5339538 term_id: GO:0015095 manual_verdict: contested_direct_activity manual_bucket: contested_direct_activity manual_reason: "The historical direct Mg2+ transporter model is contested; MAGT1's established activity is the OST-B thioredoxin-like oxidoreductase role." - organism: human gene: MAP3K5 reactome_id: R-HSA-3228469 term_id: GO:0004674 manual_verdict: reaction_mf_refine manual_bucket: broad_parent_or_duplicate manual_reason: "The protein kinase parent is redundant with a more informative MAP3K activity." - organism: human gene: MAP3K5 reactome_id: R-NUL-3299417 term_id: GO:0004674 manual_verdict: reaction_mf_refine manual_bucket: broad_parent_or_duplicate manual_reason: "This is a duplicate generic kinase parent for the MAP3K5 activity." - organism: human gene: MMAA reactome_id: R-HSA-3159259 term_id: GO:0140104 manual_verdict: wrong_executor manual_bucket: wrong_executor manual_reason: "MMAA is the GTPase that gates cobalamin transfer onto MMUT, not the molecular carrier of cobalamin cargo." - organism: human gene: PIGW reactome_id: R-HSA-162683 term_id: GO:0016747 manual_verdict: reaction_mf_refine manual_bucket: broad_parent_or_duplicate manual_reason: "The acyltransferase parent is correct but broader than the PIGW inositol-acylation MF." - organism: human gene: RDH11 reactome_id: R-HSA-975629 term_id: GO:0016616 manual_verdict: reaction_mf_refine manual_bucket: broad_parent_or_duplicate manual_reason: "The CH-OH donor oxidoreductase parent is much broader than NADP-dependent retinol dehydrogenase activity." - organism: human gene: RFT1 reactome_id: R-HSA-4570573 term_id: GO:0034202 manual_verdict: contested_direct_activity manual_bucket: contested_direct_activity manual_reason: "The disease event asserts the canonical flippase model, but the direct mechanism and ATP-dependence remain unsettled." - organism: human gene: SPHK1 reactome_id: R-HSA-428273 term_id: GO:0001727 manual_verdict: reaction_mf_refine manual_bucket: broad_parent_or_duplicate manual_reason: "The generic lipid kinase parent is subsumed by sphingosine kinase activity." - organism: human gene: SPHK2 reactome_id: R-HSA-9695949 term_id: GO:0001727 manual_verdict: reaction_mf_refine manual_bucket: broad_parent_or_duplicate manual_reason: "The generic lipid kinase parent is subsumed by sphingosine kinase activity." - organism: human gene: SRD5A3 reactome_id: R-HSA-9705714 term_id: GO:0003865 manual_verdict: nonphysiological_side_activity manual_bucket: nonphysiological_side_activity manual_reason: "The testosterone-to-DHT event reflects a weak in-vitro side activity rather than SRD5A3's physiological dolichol-pathway function." - organism: human gene: TK2 reactome_id: R-HSA-109759 term_id: GO:0019206 manual_verdict: reaction_mf_refine manual_bucket: broad_parent_or_duplicate manual_reason: "The nucleoside kinase parent is true but broader than the deoxyribonucleoside kinase activities in the TK2 event." - organism: human gene: TUSC3 reactome_id: R-HSA-5339528 term_id: GO:0015095 manual_verdict: contested_direct_activity manual_bucket: contested_direct_activity manual_reason: "TUSC3 has no demonstrated intrinsic Mg2+ channel or transporter activity; its established MF is thioredoxin-like oxidoreductase." - organism: human gene: AP4M1 reactome_id: R-HSA-5229111 term_id: GO:0008320 manual_verdict: wrong_transport_mechanism manual_bucket: wrong_transport_mechanism manual_reason: "AP-4 selects APP into vesicles but does not move APP from one side of a membrane to the other." - organism: human gene: ARHGAP11B reactome_id: R-HSA-8981637 term_id: GO:0005096 manual_verdict: activity_contradicted manual_bucket: activity_contradicted manual_reason: "The positive RHOA GAP assignment is explicitly negated by stronger direct annotations on the same gene." - organism: human gene: ATP13A1 reactome_id: R-HSA-5692462 term_id: GO:0015410 manual_verdict: wrong_go_branch manual_bucket: wrong_go_branch manual_reason: "ATP13A1 is a P-type ATPase, not an ABC-type manganese transporter." - organism: human gene: AZIN2 reactome_id: R-HSA-350598 term_id: GO:0008792 manual_verdict: activity_contradicted manual_bucket: activity_contradicted manual_reason: "The legacy arginine decarboxylase assignment is contradicted by later evidence that AZIN2 lacks ODC and ADC activity." - organism: human gene: CFTR reactome_id: R-HSA-1454916 term_id: GO:0043225 manual_verdict: obsolete_go_term manual_bucket: obsolete_go_term manual_reason: "The row uses an obsolete ATPase-coupled inorganic anion transporter term." - organism: human gene: LPCAT4 reactome_id: R-HSA-75885 term_id: GO:0003841 manual_verdict: activity_contradicted manual_bucket: activity_contradicted manual_reason: "Direct human LPCAT4 biochemistry contradicts the family LPA-to-PA activity." - organism: human gene: LPCAT4 reactome_id: R-HSA-1482635 term_id: GO:0047144 manual_verdict: activity_contradicted manual_bucket: activity_contradicted manual_reason: "Position-resolved substrate testing contradicts the 2-acyl-LPG family event for LPCAT4." - organism: human gene: RASA1 reactome_id: R-HSA-8981353 term_id: GO:0003924 manual_verdict: regulator_not_executor manual_bucket: regulator_not_executor manual_reason: "RASA1 stimulates Ras-family GTPases but is not itself the GTP-hydrolyzing enzyme in the event." - organism: human gene: RGS20 reactome_id: R-HSA-8982020 term_id: GO:0003924 manual_verdict: regulator_not_executor manual_bucket: regulator_not_executor manual_reason: "RGS20 accelerates GTP hydrolysis by G alpha(i) subunits but does not hydrolyze GTP itself." - organism: human gene: RGS20 reactome_id: R-HSA-8982021 term_id: GO:0003924 manual_verdict: regulator_not_executor manual_bucket: regulator_not_executor manual_reason: "RGS20 accelerates GTP hydrolysis by G alpha(z) but does not hydrolyze GTP itself." - organism: human gene: TXN reactome_id: R-NUL-9617742 term_id: GO:0004791 manual_verdict: wrong_executor manual_bucket: wrong_executor manual_reason: "The thioredoxin-disulfide reductase MF belongs to thioredoxin reductase rather than thioredoxin." - organism: mouse gene: Pld4 reactome_id: R-MMU-1483172 term_id: GO:0004630 manual_verdict: activity_contradicted manual_bucket: activity_contradicted manual_reason: "Experimental evidence shows PLD4 lacks authentic PLD activity, contradicting the broad Pld1-4/6 Reactome family event."