Ribosome quality control

GO annotation review of the 12 genes that rescue stalled and colliding ribosomes in human

AI Gene Review · projects/RIBOSOME_QUALITY_CONTROL · IN_PROGRESS · 2026

Bottom line

  • All 13 candidate genes are reviewed (12 RQC genes + VCP), done during the Proteostasis batches rather than under this project.
  • 430 GOA rows on the 12 RQC genes: 276 ACCEPT, 95 non-core, 20 REMOVE, 18 over-annotated, 17 MODIFY, 4 NEW.
  • Every RQC-specific row (97) was accepted or added. Corrections fall on protein binding and off-pathway terms. No module yet.

The pathway

Why this pathway

  • A surveillance pathway with sharp boundaries: sense a collision, split the ribosome, destroy the nascent chain.
  • GO already has specific terms for each stage (rescue of stalled cytosolic ribosome, RQC complex, RQC-trigger complex), so this tests whether reviews confirm good curation rather than only finding errors.
  • Disease links: NEMF variants in neurological disease; ribosome stalling in C9orf72 ALS.

Results by action

What a review looks like

LTN1 review page: the RQC-specific process GO:1990116 is accepted and marked as its defining process.

Where the corrections are

Gene REMOVE MODIFY
EDF1 7 protein binding 3: to TFIID-class TF complex binding, nuclear hormone receptor binding, Pol II TF activity
ABCE1 5 protein binding, membrane (HDA) 2: iron ion binding → 4Fe-4S cluster binding; mitochondrial matrix → mitochondrion
ANKZF1 ERAD pathway (IBA) 6: RNA endonuclease / tRNA catalytic rows → tRNA-specific ribonuclease activity
ASCC3 DNA replication (NAS), membrane (HDA) 2: → 3'-5' DNA helicase activity
ASCC2 3 protein binding, DNA replication (NAS) 2: ubiquitin binding → K63-linked polyubiquitin binding

Status and next steps

  • ✅ 13/13 candidate genes have complete reviews (no PENDING rows).
  • ⬜ Build an RQC module; six production GO-CAMs already model ZNF598 activation, GIGYF2–EIF4E2 repression, the RQC complex, NEMF CAT-tailing and ANKZF1 tRNA recycling.
  • ⬜ Update the stale "Stub" status line on the project page.

Read more: projects/RIBOSOME_QUALITY_CONTROL.md · genes/human/LTN1/ · projects/PROTEOSTASIS/batch6_selection_notes.md