Heatmap_Rank Locus_Name miRNA_Family Category Conservation_Scope Mature_5to3_Seq Tissue_Expression_Pattern THLAR_Target_Count Sample_THLAR_Targets 1 miR166 miR166 Conserved Rosids TCGGACCAGGCTTCATTCCCC High ubiquitous (Pollen/Inflorescence/Root/Leaf) 6 TAV2_LOCUS3522,TAV2_LOCUS13913,TAV2_LOCUS15361,TAV2_LOCUS21990,TAV2_LOCUS23090,TAV2_LOCUS3035 2 miR159 miR159 Conserved Rosids TTTGGATTGAAGGGAGCTCTA High ubiquitous (Pollen/Inflorescence/Root/Leaf) 1 TAV2_LOCUS19513 3 miR166.4 miR166 Conserved Rosids TCGGACCAGGCTTCATTCCCC High ubiquitous (Pollen/Inflorescence/Root/Leaf) 6 TAV2_LOCUS3522,TAV2_LOCUS13913,TAV2_LOCUS15361,TAV2_LOCUS21990,TAV2_LOCUS23090,TAV2_LOCUS3035 4 miR165.1 miR165 Conserved Brassicaceae TCGGACCAGGCTTCATCCCCC High ubiquitous (Pollen/Inflorescence/Root/Leaf) 5 TAV2_LOCUS13913,TAV2_LOCUS15361,TAV2_LOCUS21990,TAV2_LOCUS23090,TAV2_LOCUS3035 5 miR403 miR403 Conserved Rosids TTAGATTCACGCACAAACTCG High ubiquitous (Pollen/Inflorescence/Root/Leaf) 0 6 miR408 miR408 Conserved Rosids ATGCACTGCCTCTTCCCTGGC High ubiquitous; Cluster_8158 (100% mature homology to ath-miR408) 1 TAV2_LOCUS7377 7 miR398 miR398 Conserved Rosids TGTGTTCTCAGGTCACCCCTG High in Root/Leaf, moderate in Pollen/Inflorescence 0 8 miR159.1 miR159 Conserved Rosids TTTGGATTGAAGGGAGCTCTA High ubiquitous (Pollen/Inflorescence/Root/Leaf) 1 TAV2_LOCUS19513 9 miR396.1 miR396 Conserved Rosids TTCCACAGCTTTCTTGAACTT High ubiquitous (Pollen/Inflorescence/Root/Leaf) 7 TAV2_LOCUS11916,TAV2_LOCUS13594,TAV2_LOCUS13892,TAV2_LOCUS16031,TAV2_LOCUS19183,TAV2_LOCUS23382 10 miR168 miR168 Conserved Rosids TCGCTTGGTGCAGGTCGGGAA High ubiquitous (AGO1 feedback regulator) 0 11 miR166.3 miR166 Conserved Rosids TCGGACCAGGCTTCATTCCCC High in Inflorescence/Leaf, low in Root/Pollen_4-5 6 TAV2_LOCUS3522,TAV2_LOCUS13913,TAV2_LOCUS15361,TAV2_LOCUS21990,TAV2_LOCUS23090,TAV2_LOCUS3035 12 miR166.1 miR166 Conserved Rosids TCGGACCAGGCTTCATTCCCC High across most tissues 6 TAV2_LOCUS3522,TAV2_LOCUS13913,TAV2_LOCUS15361,TAV2_LOCUS21990,TAV2_LOCUS23090,TAV2_LOCUS3035 13 Cluster_1094 Novel Novel Thlaspi arvense Broad expression across Pollen/Inflorescence/Root/Leaf 0 14 Cluster_1095 Novel Novel Thlaspi arvense Broad expression across Pollen/Inflorescence/Root/Leaf 0 15 miR162.1 miR162 Conserved Rosids TCGATAAACCTCTGCATCCAG Broad high expression (DCL1 feedback regulator) 1 TAV2_LOCUS3068 16 Cluster_15766 Novel Novel Thlaspi arvense Broad high expression across tissues 0 17 miR167.1 miR167 Conserved Rosids TGAAGCTGCCAGCATGATCTA High in Inflorescence and Leaf, moderate in Root/Pollen 2 TAV2_LOCUS16836,TAV2_LOCUS23322 18 miR160 miR160 Conserved Rosids TGCCTGGCTCCCTGTATGCCA Moderate-high across tissues 5 TAV2_LOCUS15436,TAV2_LOCUS19535,TAV2_LOCUS4572,TAV2_LOCUS13658,TAV2_LOCUS23883 19 miR165 miR165 Conserved Brassicaceae TCGGACCAGGCTTCATCCCCC High in Root/Pollen/Inflorescence, moderate in Leaf 5 TAV2_LOCUS13913,TAV2_LOCUS15361,TAV2_LOCUS21990,TAV2_LOCUS23090,TAV2_LOCUS3035 20 miR166.2 miR166 Conserved Rosids TCGGACCAGGCTTCATTCCCC High in Root/Pollen/Inflorescence, moderate in Leaf 6 TAV2_LOCUS3522,TAV2_LOCUS13913,TAV2_LOCUS15361,TAV2_LOCUS21990,TAV2_LOCUS23090,TAV2_LOCUS3035 21 Cluster_16818 Novel Novel Thlaspi arvense Moderate-high in Root/Pollen/Inflorescence 0 22 miR390.1 miR390 Conserved Rosids AAGCTCAGGAGGGATAGCGCC High in Root/Pollen/Inflorescence, moderate in Leaf (TAS3 trigger) 2 TAV2_LOCUS8390,TAV2_LOCUS8392 23 miR156.5 miR156 Conserved Rosids TGACAGAAGAGAGTGAGCAC Moderate-high across tissues 8 TAV2_LOCUS12133,TAV2_LOCUS13245,TAV2_LOCUS16487,TAV2_LOCUS17655,TAV2_LOCUS22078,TAV2_LOCUS686 24 Cluster_16169 Novel Novel Thlaspi arvense High in Inflorescence/Pollen_2, moderate in Root/Leaf 0 25 miR161 miR161 Conserved Brassicaceae TCAATGCATTGAAAGTGACTA Moderate across tissues (PPR phasiRNA trigger) 7 TAV2_LOCUS5095,TAV2_LOCUS6988,TAV2_LOCUS2549,TAV2_LOCUS4999,TAV2_LOCUS5004,TAV2_LOCUS5538 26 miR156.3 miR156 Conserved Rosids TGACAGAAGAGAGTGAGCAC High in Leaf and Root, low in Inflorescence/Pollen_2 8 TAV2_LOCUS12133,TAV2_LOCUS13245,TAV2_LOCUS16487,TAV2_LOCUS17655,TAV2_LOCUS22078,TAV2_LOCUS686 27 Cluster_10911 Novel Novel Thlaspi arvense Moderate-high in Leaf/Root 0 28 miR162 miR162 Conserved Rosids TCGATAAACCTCTGCATCCAG High in Leaf and Root, moderate in Inflorescence/Pollen 1 TAV2_LOCUS3068 29 miR396 miR396 Conserved Rosids TTCCACAGCTTTCTTGAACTT High in Leaf, moderate in Root/Inflorescence; Cluster_18198 7 TAV2_LOCUS11916,TAV2_LOCUS13594,TAV2_LOCUS13892,TAV2_LOCUS16031,TAV2_LOCUS19183,TAV2_LOCUS23382 30 miR157 miR157 Conserved Brassicaceae TTGACAGAAGATAGAGAGCAC High in Root/Pollen/Leaf, moderate in Inflorescence 8 TAV2_LOCUS13245,TAV2_LOCUS17655,TAV2_LOCUS686,TAV2_LOCUS687,TAV2_LOCUS8053,TAV2_LOCUS12133 31 miR319 miR319 Conserved Rosids TTGGACTGAAGGGAGCTCCCT High in Root/Pollen_4-6/Inflorescence, moderate in Leaf 1 TAV2_LOCUS20251 32 miR157.1 miR157 Conserved Brassicaceae TTGACAGAAGATAGAGAGCAC High in Root/Pollen_4-6, lower in Inflorescence/Leaf 8 TAV2_LOCUS13245,TAV2_LOCUS17655,TAV2_LOCUS686,TAV2_LOCUS687,TAV2_LOCUS8053,TAV2_LOCUS12133 33 miR156.6 miR156 Conserved Rosids TGACAGAAGAGAGTGAGCAC High in Root/Pollen_4-6, lower in Inflorescence/Leaf 8 TAV2_LOCUS12133,TAV2_LOCUS13245,TAV2_LOCUS16487,TAV2_LOCUS17655,TAV2_LOCUS22078,TAV2_LOCUS686 34 miR156.2 miR156 Conserved Rosids TGACAGAAGAGAGTGAGCAC High in Root/Pollen_4-6, low in Inflorescence/Leaf 8 TAV2_LOCUS12133,TAV2_LOCUS13245,TAV2_LOCUS16487,TAV2_LOCUS17655,TAV2_LOCUS22078,TAV2_LOCUS686 35 miR393 miR393 Conserved Rosids TCCAAAGGGATCGCATTGATCC High in Inflorescence and Leaf, low in Root/Pollen_4-6 5 TAV2_LOCUS4099,TAV2_LOCUS16707,TAV2_LOCUS7969,TAV2_LOCUS11771,TAV2_LOCUS18979 36 miR817 miR817 Conserved Shared with rice TCTTGACCTTGTAAGACCCA High in Inflorescence and Leaf, moderate in Root 5 TAV2_LOCUS12795,TAV2_LOCUS21882,TAV2_LOCUS7857,TAV2_LOCUS15906,TAV2_LOCUS16423 37 Cluster_18440 Novel Novel Thlaspi arvense Enriched in Inflorescence/Leaf 0 38 miR160.2 miR160 Conserved Rosids TGCCTGGCTCCCTGTATGCCA Enriched in Inflorescence/Leaf 5 TAV2_LOCUS15436,TAV2_LOCUS19535,TAV2_LOCUS4572,TAV2_LOCUS13658,TAV2_LOCUS23883 39 miR160.1 miR160 Conserved Rosids TGCCTGGCTCCCTGTATGCCA Moderate in Inflorescence/Root/Leaf 5 TAV2_LOCUS15436,TAV2_LOCUS19535,TAV2_LOCUS4572,TAV2_LOCUS13658,TAV2_LOCUS23883 40 miR156.7 miR156 Conserved Rosids TGACAGAAGAGAGTGAGCAC Enriched in Root and Leaf, low in Inflorescence 8 TAV2_LOCUS12133,TAV2_LOCUS13245,TAV2_LOCUS16487,TAV2_LOCUS17655,TAV2_LOCUS22078,TAV2_LOCUS686 41 Cluster_5256 Novel Novel Thlaspi arvense Low-moderate across tissues 0 42 miR171.1 miR171 Conserved Rosids TTGAGCCGTGCCAATATCACG Low-moderate in Inflorescence/Leaf 3 TAV2_LOCUS20261,TAV2_LOCUS24000,TAV2_LOCUS4517 43 Cluster_14209 Novel Novel Thlaspi arvense Low-moderate in Inflorescence/Leaf 0 44 miR167 miR167 Conserved Rosids TGAAGCTGCCAGCATGATCTA Enriched in Inflorescence/Pollen_2 and Leaf 2 TAV2_LOCUS16836,TAV2_LOCUS23322 45 miR172.1 miR172 Conserved Rosids AGAATCTTGATGATGCTGCAT Enriched in Inflorescence/Pollen_2 and Leaf 7 TAV2_LOCUS18783,TAV2_LOCUS23360,TAV2_LOCUS12280,TAV2_LOCUS13511,TAV2_LOCUS15398,TAV2_LOCUS5241 46 miR172.2 miR172 Conserved Rosids AGAATCTTGATGATGCTGCAT Enriched in Inflorescence/Pollen_2 7 TAV2_LOCUS18783,TAV2_LOCUS23360,TAV2_LOCUS12280,TAV2_LOCUS13511,TAV2_LOCUS15398,TAV2_LOCUS5241 47 miR171 miR171 Conserved Rosids TTGAGCCGTGCCAATATCACG Enriched in Inflorescence and Leaf_4-6 3 TAV2_LOCUS20261,TAV2_LOCUS24000,TAV2_LOCUS4517 48 miR156.4 miR156 Conserved Rosids TGACAGAAGAGAGTGAGCAC Moderate in Root and Leaf_4-6 8 TAV2_LOCUS12133,TAV2_LOCUS13245,TAV2_LOCUS16487,TAV2_LOCUS17655,TAV2_LOCUS22078,TAV2_LOCUS686 49 miR319.1 miR319 Conserved Rosids TTGGACTGAAGGGAGCTCCCT Enriched in Leaf_4-6 and Inflorescence 1 TAV2_LOCUS20251 50 miR164.1 miR164 Conserved Rosids TGGAGAAGCAGGGCACGTGCA Moderate across Inflorescence/Root/Leaf 6 TAV2_LOCUS20428,TAV2_LOCUS5185,TAV2_LOCUS11328,TAV2_LOCUS2785,TAV2_LOCUS11048,TAV2_LOCUS21106 51 Cluster_2822 Novel Novel Thlaspi arvense Moderate across Inflorescence/Root/Leaf 0 52 miR156.1 miR156 Conserved Rosids TGACAGAAGAGAGTGAGCAC Moderate in Inflorescence, low in Root/Leaf 8 TAV2_LOCUS12133,TAV2_LOCUS13245,TAV2_LOCUS16487,TAV2_LOCUS17655,TAV2_LOCUS22078,TAV2_LOCUS686 53 miR171.2 miR171 Conserved Rosids TTGAGCCGTGCCAATATCACG Low across tissues 3 TAV2_LOCUS20261,TAV2_LOCUS24000,TAV2_LOCUS4517 54 miR156 miR156 Conserved Rosids TGACAGAAGAGAGTGAGCAC Moderate in Leaf_4-6, low in other tissues 8 TAV2_LOCUS12133,TAV2_LOCUS13245,TAV2_LOCUS16487,TAV2_LOCUS17655,TAV2_LOCUS22078,TAV2_LOCUS686 55 miR395 miR395 Conserved Rosids CTGAAGTGTTTGGGGGAACTC Low-moderate in Root/Leaf (Sulfate assimilation regulator) 3 TAV2_LOCUS14532,TAV2_LOCUS7508,TAV2_LOCUS21835 56 Cluster_229 Novel Novel Thlaspi arvense Moderate in Inflorescence, low in Root/Leaf 0 57 Cluster_16503 Novel Novel Thlaspi arvense Low-moderate in Inflorescence/Root 0 58 miR164.2 miR164 Conserved Rosids TGGAGAAGCAGGGCACGTGCA Low-moderate in Inflorescence/Leaf 6 TAV2_LOCUS20428,TAV2_LOCUS5185,TAV2_LOCUS11328,TAV2_LOCUS2785,TAV2_LOCUS11048,TAV2_LOCUS21106 59 Cluster_10185 Novel Novel Thlaspi arvense Low in Root/Leaf 0 60 miR169.1 miR169 Conserved Rosids TGAGCCAAGGATGACTTGCCG Low in Root/Leaf 0 61 miR169 miR169 Conserved Rosids TGAGCCAAGGATGACTTGCCG Root-enriched 0 62 Cluster_10717 Novel Novel Thlaspi arvense Enriched in Inflorescence/Pollen_2 and Root 0 63 miR397 miR397 Conserved Rosids TCATTGAGTGCATCGTTGATG Root-enriched (Laccase regulator) 2 TAV2_LOCUS14099,TAV2_LOCUS22308 64 Cluster_3759 Novel Novel Thlaspi arvense Root-enriched 0 65 Cluster_7729 Novel Novel Thlaspi arvense Inflorescence/Pollen_2-enriched 0 66 miR172 miR172 Conserved Rosids AGAATCTTGATGATGCTGCAT Inflorescence/Pollen_2-specific (AP2/TOE floral regulator) 7 TAV2_LOCUS18783,TAV2_LOCUS23360,TAV2_LOCUS12280,TAV2_LOCUS13511,TAV2_LOCUS15398,TAV2_LOCUS5241 67 Cluster_13389 Novel Novel Thlaspi arvense Low in Leaf/Root 0 68 Cluster_11286 Novel Novel Thlaspi arvense Low in Leaf 0 69 Cluster_10472 Novel Novel Thlaspi arvense Low in Inflorescence/Root 0 70 miR390 miR390 Conserved Rosids AAGCTCAGGAGGGATAGCGCC Low in Inflorescence/Root 2 TAV2_LOCUS8390,TAV2_LOCUS8392 71 miR164 miR164 Conserved Rosids TGGAGAAGCAGGGCACGTGCA Low in Inflorescence 6 TAV2_LOCUS20428,TAV2_LOCUS5185,TAV2_LOCUS11328,TAV2_LOCUS2785,TAV2_LOCUS11048,TAV2_LOCUS21106 72 miR399 miR399 Conserved Rosids TGCCAAAGGAGAGTTGCCCTG Low in Inflorescence (Phosphate starvation PHO2 regulator) 1 TAV2_LOCUS15095