{
 "gene": "AATF",
 "run_date": "2026-06-09T22:02:36",
 "timeline": {
  "current_model": "AATF/Che-1 is a multifunctional nuclear phosphoprotein that binds RNA polymerase II (and I), Rb, and multiple transcription factors; in unstressed cells it promotes proliferation by displacing HDAC1 from Rb/E2F and Sp1 complexes to activate E2F target genes and p21, while upon genotoxic stress it is phosphorylated by ATM/ATR, Chk2, MK2, CK2, and HIPK2 at distinct residues\u2014triggering nuclear translocation, promoter switching from E2F targets to p53/p21 loci to activate cell-cycle arrest, or (when phosphorylated by MK2) binding PUMA/BAX/BAK promoters to repress p53-driven apoptosis\u2014and is ultimately degraded via Pin1/HDM2/HIPK2-mediated ubiquitylation during apoptosis; AATF also forms the ANN complex (with NGDN and NOL10) in nucleoli to support 40S ribosomal subunit biogenesis and rRNA processing, localizes to paraspeckles via NEAT1_2 to resolve R-loops and suppress interferon activation, stabilizes XRCC4 for NHEJ repair (released by ATM-mediated Ser189 phosphorylation at DNA breaks), and interacts with partners including Par-4, Tau, Cdk5, c-Jun, AIF, SAGA subunits, and histones to regulate apoptosis, neuronal function, and chromatin acetylation across multiple cellular contexts."
 },
 "narrative": {
  "mechanism_profile": {
   "molecular_activity": [
    {
     "term_id": "GO:0140110",
     "term_label": "transcription regulator activity",
     "supporting_discovery_ids": [
      0,
      2,
      3,
      7,
      20,
      25
     ]
    },
    {
     "term_id": "GO:0042393",
     "term_label": "histone binding",
     "supporting_discovery_ids": [
      26,
      27
     ]
    },
    {
     "term_id": "GO:0003723",
     "term_label": "RNA binding",
     "supporting_discovery_ids": [
      28
     ]
    },
    {
     "term_id": "GO:0060090",
     "term_label": "molecular adaptor activity",
     "supporting_discovery_ids": [
      2,
      3,
      21,
      31
     ]
    },
    {
     "term_id": "GO:0098772",
     "term_label": "molecular function regulator activity",
     "supporting_discovery_ids": [
      2,
      25,
      31
     ]
    }
   ],
   "localization": [
    {
     "term_id": "GO:0005730",
     "term_label": "nucleolus",
     "supporting_discovery_ids": [
      16,
      21,
      25
     ]
    },
    {
     "term_id": "GO:0005634",
     "term_label": "nucleus",
     "supporting_discovery_ids": [
      2,
      7,
      9,
      20
     ]
    },
    {
     "term_id": "GO:0005654",
     "term_label": "nucleoplasm",
     "supporting_discovery_ids": [
      28
     ]
    },
    {
     "term_id": "GO:0005815",
     "term_label": "microtubule organizing center",
     "supporting_discovery_ids": [
      17
     ]
    },
    {
     "term_id": "GO:0005829",
     "term_label": "cytosol",
     "supporting_discovery_ids": [
      4,
      15
     ]
    }
   ],
   "pathway": [
    {
     "term_id": "R-HSA-73894",
     "term_label": "DNA Repair",
     "supporting_discovery_ids": [
      7,
      14,
      31
     ]
    },
    {
     "term_id": "R-HSA-1640170",
     "term_label": "Cell Cycle",
     "supporting_discovery_ids": [
      2,
      7,
      17,
      20
     ]
    },
    {
     "term_id": "R-HSA-5357801",
     "term_label": "Programmed Cell Death",
     "supporting_discovery_ids": [
      8,
      15,
      16,
      18,
      29
     ]
    },
    {
     "term_id": "R-HSA-74160",
     "term_label": "Gene expression (Transcription)",
     "supporting_discovery_ids": [
      2,
      3,
      25,
      26
     ]
    },
    {
     "term_id": "R-HSA-8953854",
     "term_label": "Metabolism of RNA",
     "supporting_discovery_ids": [
      21,
      25
     ]
    },
    {
     "term_id": "R-HSA-1643685",
     "term_label": "Disease",
     "supporting_discovery_ids": [
      23,
      24,
      26
     ]
    },
    {
     "term_id": "R-HSA-8953897",
     "term_label": "Cellular responses to stimuli",
     "supporting_discovery_ids": [
      12,
      19,
      22,
      28
     ]
    }
   ],
   "complexes": [
    "ANN complex (AATF-NGDN-NOL10)",
    "SAGA HAT module",
    "RNA polymerase I/UBF rDNA transcription complex"
   ],
   "partners": [
    "RB1",
    "TP53",
    "HDAC1",
    "HDM2",
    "PIN1",
    "PARP1",
    "XRCC4",
    "NGDN"
   ],
   "other_free_text": []
  }
 },
 "prefetch_data": {
  "uniprot": {
   "accession": "Q9NY61",
   "full_name": "Protein AATF"
  }
 },
 "evaluation": {
  "pairwise": "win",
  "faith_supported": 9,
  "faith_total": 9,
  "faith_pct": 100.0
 },
 "cost": {
  "total_usd": 0.24222
 }
}