{
 "gene": "ACADM",
 "run_date": "2026-06-09T22:02:38",
 "timeline": {
  "current_model": "ACADM (MCAD) encodes a mitochondrial homotetrameric flavoenzyme that catalyzes the first step of \u03b2-oxidation for medium-chain (C4\u2013C12) acyl-CoAs via an FAD-dependent mechanism generating a charge-transfer complex with the enoyl-CoA product; newly imported MCAD monomers are chaperoned sequentially by mitochondrial hsp70 and hsp60 in an ATP-dependent folding pathway before assembling into active tetramers, a process disrupted by the prevalent K304E/K329E disease mutation which impairs the hsp60 release step and subunit docking through charge repulsion; the enzyme also undergoes mitochondrial post-translational modification (likely phosphorylation) after transit peptide removal, is regulated transcriptionally by the CAV1/SREBP1 axis, and is phosphorylated downstream of PINK1 kinase, with loss of MCAD function causing accumulation of medium-chain acylcarnitines and fatty acids, impaired hepatic glucose homeostasis under metabolic stress, and disrupted host processing of microbiota-derived metabolites including hippurate."
 },
 "narrative": {
  "mechanism_profile": {
   "molecular_activity": [
    {
     "term_id": "GO:0016491",
     "term_label": "oxidoreductase activity",
     "supporting_discovery_ids": [
      7,
      16,
      21
     ]
    },
    {
     "term_id": "GO:0016740",
     "term_label": "transferase activity",
     "supporting_discovery_ids": [
      7
     ]
    }
   ],
   "localization": [
    {
     "term_id": "GO:0005739",
     "term_label": "mitochondrion",
     "supporting_discovery_ids": [
      4,
      6
     ]
    }
   ],
   "pathway": [
    {
     "term_id": "R-HSA-1430728",
     "term_label": "Metabolism",
     "supporting_discovery_ids": [
      7,
      21,
      14
     ]
    },
    {
     "term_id": "R-HSA-392499",
     "term_label": "Metabolism of proteins",
     "supporting_discovery_ids": [
      4
     ]
    }
   ],
   "complexes": [],
   "partners": [
    "HSPD1",
    "HSPA9",
    "ETF",
    "PINK1",
    "SREBF1",
    "CAV1"
   ],
   "other_free_text": []
  }
 },
 "prefetch_data": {
  "uniprot": {
   "accession": "P11310",
   "full_name": "Medium-chain specific acyl-CoA dehydrogenase, mitochondrial"
  }
 },
 "evaluation": {
  "pairwise": "win",
  "faith_supported": 8,
  "faith_total": 8,
  "faith_pct": 100.0
 },
 "cost": {
  "total_usd": 0.225542
 }
}