{
 "gene": "LDHA",
 "run_date": "2026-06-10T02:59:49",
 "timeline": {
  "current_model": "LDHA is established as the key glycolytic enzyme catalyzing pyruvate-to-lactate conversion using NADH; its activity is regulated by multiple PTMs (phosphorylation at Y10 by HER2/Src/FGFR1, palmitoylation at C163 by ZDHHC9, succinylation at K155 inhibited by SIRT5/promoted by lncRNA GLTC, and S-nitrosylation at C163/C293 by BH4/NO), and its transcription is activated by HIF-1/2\u03b1, FOXM1, c-Myc, JMJD2A, SIX1, YY1, and FOXQ1, and repressed by KLF4, FOXO4, and miR-34a/miR-142-3p among others; beyond glycolysis, LDHA exerts noncanonical functions including activating Rac1 GTPase (by sequestering Rac1-GTP from GAPs), binding eEF2 in an NADH-dependent manner to control mRNA translation in megakaryocytes, producing lactate that drives histone lactylation (H3K18la) of downstream gene promoters, promoting NLRP3 lactylation to induce pyroptosis, and contributing to ROS generation and NF-\u03baB/I\u03baB-\u03b6 signaling in chondrocytes."
 },
 "narrative": {
  "mechanism_profile": {
   "molecular_activity": [
    {
     "term_id": "GO:0016491",
     "term_label": "oxidoreductase activity",
     "supporting_discovery_ids": [
      0,
      4,
      5,
      6,
      8
     ]
    },
    {
     "term_id": "GO:0140096",
     "term_label": "catalytic activity, acting on a protein",
     "supporting_discovery_ids": [
      23,
      24,
      25,
      35
     ]
    },
    {
     "term_id": "GO:0098772",
     "term_label": "molecular function regulator activity",
     "supporting_discovery_ids": [
      1,
      3
     ]
    }
   ],
   "localization": [
    {
     "term_id": "GO:0005829",
     "term_label": "cytosol",
     "supporting_discovery_ids": [
      3
     ]
    }
   ],
   "pathway": [
    {
     "term_id": "R-HSA-1430728",
     "term_label": "Metabolism",
     "supporting_discovery_ids": [
      6,
      8,
      14,
      20
     ]
    },
    {
     "term_id": "R-HSA-168256",
     "term_label": "Immune System",
     "supporting_discovery_ids": [
      12,
      36,
      33
     ]
    },
    {
     "term_id": "R-HSA-4839726",
     "term_label": "Chromatin organization",
     "supporting_discovery_ids": [
      24,
      25,
      35
     ]
    },
    {
     "term_id": "R-HSA-162582",
     "term_label": "Signal Transduction",
     "supporting_discovery_ids": [
      1,
      27,
      28,
      29
     ]
    }
   ],
   "complexes": [],
   "partners": [
    "RAC1",
    "EEF2",
    "SIRT5",
    "ZDHHC9",
    "FKBP10",
    "TXNRD1",
    "NLRP3",
    "KCNK1"
   ],
   "other_free_text": []
  }
 },
 "prefetch_data": {
  "uniprot": {
   "accession": "P00338",
   "full_name": "L-lactate dehydrogenase A chain"
  }
 },
 "evaluation": {
  "pairwise": "tie",
  "faith_supported": 6,
  "faith_total": 7,
  "faith_pct": 85.71428571428571
 },
 "cost": {
  "total_usd": 0.246737
 }
}