{
 "gene": "PKM",
 "run_date": "2026-06-10T06:43:35",
 "timeline": {
  "current_model": "PKM2 is a multifunctional glycolytic enzyme that, in its cytoplasmic tetrameric form, catalyzes the conversion of PEP to pyruvate; its dimer/monomer forms can translocate to the nucleus where it acts as a protein kinase (phosphorylating histone H3-T11, Bub3-Y207, MLC2-Y118, and PGAM1-H11 via PEP-dependent mechanisms), a co-transcriptional activator (binding \u03b2-catenin, STAT3, NF-\u03baB, and Oct4 to regulate gene expression), and a non-canonical RNA-binding protein (interacting with RNA G-quadruplex structures to regulate pre-mRNA expression); its oligomeric state, subcellular localization, and activities are tightly regulated by post-translational modifications including phosphorylation (Aurora B at T45, PINK1 at S127, Src at Y105), acetylation (PCAF at K305 targeting it for autophagy; p300 at K433 promoting nuclear entry), sumoylation, glutathionylation (Cys423/424), lactylation (K62 promoting tetramerization), palmitoylation (C31 impairing tetramerization), and hydroxylation (Jmjd4 at K66 promoting CMA degradation), as well as allosteric regulators and interactions with binding partners such as MFN2, PHGDH, Annexin A5, FSTL1, CoA, and integrin \u03b1v\u03b23."
 },
 "narrative": {
  "mechanism_profile": {
   "molecular_activity": [
    {
     "term_id": "GO:0140096",
     "term_label": "catalytic activity, acting on a protein",
     "supporting_discovery_ids": [
      1,
      2,
      3,
      14
     ]
    },
    {
     "term_id": "GO:0140110",
     "term_label": "transcription regulator activity",
     "supporting_discovery_ids": [
      0,
      8,
      33
     ]
    },
    {
     "term_id": "GO:0003723",
     "term_label": "RNA binding",
     "supporting_discovery_ids": [
      15,
      16
     ]
    },
    {
     "term_id": "GO:0016740",
     "term_label": "transferase activity",
     "supporting_discovery_ids": [
      1,
      14
     ]
    }
   ],
   "localization": [
    {
     "term_id": "GO:0005634",
     "term_label": "nucleus",
     "supporting_discovery_ids": [
      0,
      1,
      8,
      15,
      30,
      33
     ]
    },
    {
     "term_id": "GO:0005829",
     "term_label": "cytosol",
     "supporting_discovery_ids": [
      7,
      18,
      34
     ]
    },
    {
     "term_id": "GO:0005739",
     "term_label": "mitochondrion",
     "supporting_discovery_ids": [
      7
     ]
    },
    {
     "term_id": "GO:0005840",
     "term_label": "ribosome",
     "supporting_discovery_ids": [
      16
     ]
    },
    {
     "term_id": "GO:0005576",
     "term_label": "extracellular region",
     "supporting_discovery_ids": [
      21
     ]
    }
   ],
   "pathway": [
    {
     "term_id": "R-HSA-1430728",
     "term_label": "Metabolism",
     "supporting_discovery_ids": [
      4,
      9,
      18,
      23,
      29
     ]
    },
    {
     "term_id": "R-HSA-74160",
     "term_label": "Gene expression (Transcription)",
     "supporting_discovery_ids": [
      0,
      1,
      8,
      33
     ]
    },
    {
     "term_id": "R-HSA-168256",
     "term_label": "Immune System",
     "supporting_discovery_ids": [
      6,
      8,
      19,
      29
     ]
    },
    {
     "term_id": "R-HSA-1640170",
     "term_label": "Cell Cycle",
     "supporting_discovery_ids": [
      2,
      3,
      10
     ]
    },
    {
     "term_id": "R-HSA-8953854",
     "term_label": "Metabolism of RNA",
     "supporting_discovery_ids": [
      15,
      16
     ]
    },
    {
     "term_id": "R-HSA-9612973",
     "term_label": "Autophagy",
     "supporting_discovery_ids": [
      4,
      12,
      36
     ]
    },
    {
     "term_id": "R-HSA-8953897",
     "term_label": "Cellular responses to stimuli",
     "supporting_discovery_ids": [
      31,
      32
     ]
    }
   ],
   "complexes": [],
   "partners": [
    "CTNNB1",
    "STAT3",
    "MFN2",
    "PHGDH",
    "PGAM1",
    "MARCH2",
    "ANXA5",
    "FSTL1"
   ],
   "other_free_text": []
  }
 },
 "prefetch_data": {
  "uniprot": {
   "accession": "P14618",
   "full_name": "Pyruvate kinase PKM"
  }
 },
 "evaluation": {
  "pairwise": "win",
  "faith_supported": 8,
  "faith_total": 8,
  "faith_pct": 100.0
 },
 "cost": {
  "total_usd": 0.305812
 }
}