module: dmel_glutamyl_trna_amidotransferase
title: Mitochondrial glutamyl-tRNA(Gln) amidotransferase (GatCAB) in Drosophila melanogaster
label: GatCAB amidotransferase
module_type: PROTEIN_COMPLEX
description: >-
  In mitochondria of animals there is no glutaminyl-tRNA synthetase. Instead,
  mitochondrial tRNA-Gln is first mischarged with glutamate by the
  non-discriminating mitochondrial glutamyl-tRNA synthetase, and the
  heterotrimeric GatCAB amidotransferase converts the bound glutamate to
  glutamine. GatA is a glutaminase that releases ammonia from glutamine, GatB
  phosphorylates the glutamate and transfers the ammonia to form Gln-tRNA(Gln),
  and GatC is a small subunit that stabilizes the complex.
summary: >-
  Mitochondrial GatA-GatB-GatC complex that forms Gln-tRNA(Gln) by
  transamidation.
concepts:
  - {id: GO:0030956, label: glutamyl-tRNA(Gln) amidotransferase complex}
cellular_components:
  - {id: GO:0005739, label: mitochondrion}
notes: >-
  Modelled one part per subunit. The glutaminyl-tRNA synthase
  (glutamine-hydrolyzing) activity (GO:0050567) belongs to the assembled
  complex and is recorded on a complex-level annoton whose active units are
  GatA (glutaminase site) and GatB (kinase and transamidation site); GatC has
  no catalytic site and is not an active unit. GatA and GatB both carry
  GO:0050567 in GOA (IBA, ISS). The mitochondrial glutamyl-tRNA synthetase
  that provides the substrate is outside this module.
complex_term: {id: GO:0030956, label: glutamyl-tRNA(Gln) amidotransferase complex}
complex_function:
  function: {id: GO:0050567, label: glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity}
  active_units: [GatA, GatB]
  processes:
  - {id: 'GO:0070127', label: tRNA aminoacylation for mitochondrial protein translation}
  locations:
  - {id: GO:0005739, label: mitochondrion}
  role_description: >-
    Converts Glu-tRNA(Gln), made by the non-discriminating mitochondrial
    glutamyl-tRNA synthetase, to Gln-tRNA(Gln) using glutamine as the amide
    donor and ATP.
parts:
- id: gata_subunit
  label: GatA subunit
  role: glutaminase subunit
  genes: [GatA]
  processes:
  - {id: 'GO:0070127', label: tRNA aminoacylation for mitochondrial protein translation}
- id: gatb_subunit
  label: GatB subunit
  role: kinase and transamidation subunit
  genes: [GatB]
  processes:
  - {id: 'GO:0070127', label: tRNA aminoacylation for mitochondrial protein translation}
- id: gatc_subunit
  label: GatC subunit
  role: stabilizing subunit
  genes: [GatC]
  processes:
  - {id: 'GO:0070127', label: tRNA aminoacylation for mitochondrial protein translation}
group: GATCAB