module: dmel_kmn_network
title: KMN network of the outer kinetochore in Drosophila melanogaster
label: KMN network
module_type: PROTEIN_COMPLEX
description: >-
  The KMN network (Knl1, Mis12 complex and Ndc80 complex) is the core
  microtubule-binding module of the outer kinetochore. The Mis12 complex links
  the network to the centromere-proximal inner kinetochore; in Drosophila it
  binds the CENP-C homolog Cenp-C, because flies lack the CENP-T pathway. The
  Ndc80 complex binds spindle microtubules directly and forms load-bearing
  attachments. Knl1 (Spc105R in Drosophila) provides a scaffold for spindle
  assembly checkpoint proteins and for protein phosphatase 1. Drosophila has
  two Nnf1 paralogs, Nnf1a and Nnf1b, which form alternative Mis12 complexes.
summary: >-
  Spc105R (Knl1), Mis12 and Ndc80 complexes that form the
  microtubule-attachment core of the outer kinetochore.
concepts:
  - {id: GO:0000940, label: outer kinetochore}
cellular_components:
  - {id: GO:0000776, label: kinetochore}
parts:
  - id: knl1
    label: Knl1 (Spc105R)
    role: checkpoint scaffold
    group: KNL1
    complex_term: {id: GO:0180019, label: Knl1/Spc105 complex}
  - id: mis12_complex
    label: Mis12 complex
    role: inner-kinetochore linker
    variant_sets:
      - id: mis12_variants
        label: Nnf1a or Nnf1b Mis12 complex
        selection: ONE_OR_MORE
        variants:
          - {id: mis12_nnf1a, label: Mis12 complex Nnf1a variant, group: MIS12A, complex_term: {id: GO:0000444, label: MIS12/MIND type complex}}
          - {id: mis12_nnf1b, label: Mis12 complex Nnf1b variant, group: MIS12B, complex_term: {id: GO:0000444, label: MIS12/MIND type complex}}
  - id: ndc80_complex
    label: Ndc80 complex
    role: microtubule attachment
    group: NDC80C
    complex_term: {id: GO:0031262, label: Ndc80 complex}
    function: {id: GO:0008017, label: microtubule binding}
connections:
  - {source: mis12_complex, target: ndc80_complex, connection_type: POSITIVELY_REGULATES, description: The Mis12 complex recruits the Ndc80 complex to the kinetochore.}
  - {source: mis12_complex, target: knl1, connection_type: POSITIVELY_REGULATES, description: The Mis12 complex recruits Spc105R/Knl1 to the kinetochore.}
notes: >-
  FlyBase lists Ndc80, Nuf2 and Spc25 in the Ndc80 complex group. The
  microtubule binding function is placed on the Ndc80 complex as a whole.
  Checkpoint proteins recruited by Spc105R (Bub1, BubR1, Bub3) and the RZZ
  complex (dmel_rzz_complex) are outside the module.