# PN dossier: BNIP3L

- review_batch: proteostasis-batch-2026-06-06
- review_yaml: genes/human/BNIP3L/BNIP3L-ai-review.yaml
- PN workbook rows: 2

## PN row 1: Autophagy-Lysosome Pathway | Autophagophore initiation and elongation | Class 3 PI3K complex 1, direct | Modulator of class 3 PI3K complex 1 activity | Modulator of BECN1 availability
- UniProt: O60238
- In branches: ALP
- Notes: Mitochondrial membrane protein. Inhibits RHEB, also releases BECN1 from complexes with Bcl2 or BclXL. Also is a receptor for mitophagy by binding to LC3 through an LIR domain and enhances autophagy in response to hypoxia.
- PN references (titles):
    - Atypical BH3-domains of BNIP3 and BNIP3L lead to autophagy in hypoxia (tandfonline.com)
    - A brief overview of BNIP3L/NIX receptor‐mediated mitophagy - Marinković - 2021 - FEBS Open Bio - Wiley Online Library
    - The dynamics of mitochondrial autophagy at the initiation stage | Biochemical Society Transactions | Portland Press
- PN-node mapping records (path + ancestors):
    - [subtype] Autophagy-Lysosome Pathway|Autophagophore initiation and elongation|Class 3 PI3K complex 1, direct|Modulator of class 3 PI3K complex 1 activity|Modulator of BECN1 availability
        status=context_only scope=too_broad_to_propagate GO=[GO:0035032 phosphatidylinositol 3-kinase complex, class III]
        rationale: Reviewed as a class-III PI3K complex context or regulator bucket. This node is useful for curator interpretation, but it should not project cellular-component membership; only explicit complex-component leaves propagate to GO complex terms.
    - [type] Autophagy-Lysosome Pathway|Autophagophore initiation and elongation|Class 3 PI3K complex 1, direct|Modulator of class 3 PI3K complex 1 activity
        status=context_only scope=too_broad_to_propagate GO=[GO:0035032 phosphatidylinositol 3-kinase complex, class III]
        rationale: Reviewed as a class-III PI3K complex context or regulator bucket. This node is useful for curator interpretation, but it should not project cellular-component membership; only explicit complex-component leaves propagate to GO complex terms.
    - [group] Autophagy-Lysosome Pathway|Autophagophore initiation and elongation|Class 3 PI3K complex 1, direct
        status=context_only scope=too_broad_to_propagate GO=[GO:0035032 phosphatidylinositol 3-kinase complex, class III]
        rationale: Reviewed as a class-III PI3K complex context or regulator bucket. This node is useful for curator interpretation, but it should not project cellular-component membership; only explicit complex-component leaves propagate to GO complex terms.
    - [class] Autophagy-Lysosome Pathway|Autophagophore initiation and elongation
        status=context_only scope=too_broad_to_propagate GO=[GO:0016236 macroautophagy]
        rationale: This class is a real macroautophagy context, but its descendants include core factors, component buckets, upstream modulators, localization roles, and residual categories. Projecting generic macroautophagy from this ancestor creates TRAPP-like overpropagation, so candidate GO annotations must come from narrower curated nodes.
    - [branch] Autophagy-Lysosome Pathway
        status=no_mapping scope= GO=[]
        rationale: Reviewed as the top-level PN branch. It is a project taxonomy umbrella rather than a direct GO assertion; all propagation must come from manually curated child nodes.

## PN row 2: Autophagy-Lysosome Pathway | Autophagy substrate selection | Selective autophagy receptor | Mitophagy
- UniProt: O60238
- In branches: ALP
- Notes: Mitochondrial membrane protein. Inhibits RHEB, also releases BECN1 from complexes with Bcl2 or BclXL. Also is a receptor for mitophagy by binding to LC3 through an LIR domain and enhances autophagy in response to hypoxia.
- PN references (titles):
    - Atypical BH3-domains of BNIP3 and BNIP3L lead to autophagy in hypoxia (tandfonline.com)
    - A brief overview of BNIP3L/NIX receptor‐mediated mitophagy - Marinković - 2021 - FEBS Open Bio - Wiley Online Library
    - The dynamics of mitochondrial autophagy at the initiation stage | Biochemical Society Transactions | Portland Press
- PN-node mapping records (path + ancestors):
    - [type] Autophagy-Lysosome Pathway|Autophagy substrate selection|Selective autophagy receptor|Mitophagy
        status=mapped scope=ok_for_propagation_to_go GO=[GO:0000423 mitophagy]
        rationale: This PN path denotes selective-autophagy receptors for mitochondrial cargo. The source category is a mechanistic sub-role within mitophagy, so propagation rather than exact equivalence is the correct scope.
    - [group] Autophagy-Lysosome Pathway|Autophagy substrate selection|Selective autophagy receptor
        status=no_mapping scope= GO=[]
        rationale: Reviewed as a broad PN taxonomy container. The descendants mix components, regulators, context labels, and mechanistic leaves, so propagation should come only from narrower curated nodes.
    - [class] Autophagy-Lysosome Pathway|Autophagy substrate selection
        status=no_mapping scope= GO=[]
        rationale: Reviewed as a broad substrate-selection container. GO has useful targets for specific receptor, cargo-adaptor, and selective-autophagy leaves, but this class mixes marking, recognition, receptor regulation, and unknown roles and should not propagate as one term.
    - [branch] Autophagy-Lysosome Pathway
        status=no_mapping scope= GO=[]
        rationale: Reviewed as the top-level PN branch. It is a project taxonomy umbrella rather than a direct GO assertion; all propagation must come from manually curated child nodes.

## Projected GO annotations (1)
- GO:0000423 mitophagy | scope=ok_for_propagation_to_go | goa_status=supported_by_goa_regulation | from=Autophagy-Lysosome Pathway|Autophagy substrate selection|Selective autophagy receptor|Mitophagy