## AATF
- **UniProt:** Q9NY61 · **batch:** proteostasis-batch-2026-06-03 · **review status:** COMPLETE
- **PN placement:** `Translation|Cytosolic translation|Ribosome biogenesis factor|pre-60S complex|ANN complex` ; **PN-node mapping:** subtype (ANN complex)=no_mapping; type `pre-60S complex`=mapped→**GO:0030687 preribosome, large subunit precursor** (new_to_goa); group `Ribosome biogenesis factor`=mapped→GO:0042254 (entailed_by_goa_closure); class/branch context_only.
- **Consistency:** **CONTRADICTION (subunit mismatch).** PN places AATF in a **pre-60S / large-subunit** bucket ("pre-60S complex|ANN complex") and projects GO:0030687, which OLS confirms is the **large (66S/pre-60S) subunit precursor**. The review, deep research, and cryo-EM evidence (PMID:34516797) place AATF in the **small-subunit (SSU) processome / pre-40S** (GO:0032040, GO:0042274, GO:0030490). The review explicitly states: "Available structural and GOA evidence places AATF in SSU/pre-40S maturation, not in pre-60S large-subunit precursor particles." So the PN-projected GO:0030687 is wrong for AATF. The group-level GO:0042254 ribosome biogenesis is consistent (entailed). Transcription-coactivator role (Che-1) is well captured but outside the PN's translation framing.
- **PN story / NEW pressure:** PN's biogenesis story is right at the group level (ribosome biogenesis) but mis-specified at the type level (LSU vs SSU). GO:0030687 verified real but biologically incorrect for AATF; the correct complex term (GO:0032040 SSU processome) is **already captured**. No defensible NEW term to add. Conclusion: **PN over-reaches** at the pre-60S type node.
- **Mapping strategy:** The PN type-node `pre-60S complex` mapping should NOT propagate GO:0030687 to AATF. Either AATF is mis-bucketed (belongs under an SSU-processome node) or the projection must be suppressed for this gene. Like the TOMM20/HSPA8/RAB7A precedent, the projected term is rejected — here not merely as broader but as the wrong subunit.
- **Evidence alignment:** PN dossier lists no reference titles. Review centers on PMID:34516797 (cryo-EM SSU processome) and NGDN co-membership — none of which support a pre-60S placement. Divergence is conceptual (LSU vs SSU), not citation-level.
- **Verdict:** Subunit contradiction — PN over-reaches (LSU projection on an SSU factor); review correctly rejects pre-60S. **Recommended edits:** [MAP] Do not project GO:0030687 to AATF; re-bucket AATF under an SSU-processome/pre-40S PN node or suppress the pre-60S type mapping for this gene.