## ANKZF1

- **UniProt:** Q9H8Y5 · **batch:** proteostasis-batch-2026-06-03 (Falcon DR 2026-06-07) · **review status:** COMPLETE
- **PN placement:** 3 rows, MI/TR/UPS. (1) `Mitochondrial proteostasis|Organelle-specific protein degradation|Vms pathway`; (2) `Translation|Cytosolic translation|Ribosome-associated QC|VCP system for RQC`; (3) `UPS|VCP and associated proteins|adaptors|VIM|ankyrin repeats`. **PN-node mapping:** mito class=`mapped`→GO:0035694 mito protein catabolic (new_to_goa); RQC group=`mapped`→GO:0006515 protein QC (already_in_goa_exact); VCP-adaptor nodes=`no_mapping`/`context_only` (GO:0034098, GO:0043335).
- **Consistency:** Mostly consistent, with one deliberate PN-vs-review divergence (see below). DR ↔ notes ↔ YAML agree: ANKZF1 is a cytosolic RQC tRNA endonuclease (acceptor-arm/3'-CCA cleavage on 60S RQC), VCP/VIM cofactor.
- **PN story / NEW pressure:** PN projects two ok_for_propagation terms. GO:0006515 (already_in_goa) — review ACCEPTs (multiple IDA, PMID:29632312/30244831). **GO:0035694 mitochondrial protein catabolic process (PN new_to_goa, verified real): review DECLINES.** Notes find no direct human evidence ANKZF1 catalyzes mito protein catabolism; mito link is stress translocation/RQC-of-OMM-mRNAs (PMID:28302725, 38388640), not catabolism. Conclude: GO:0035694 over-reaches for this gene → already-captured story is RQC (GO:0006515); mito-catabolism not supported.
- **Mapping strategy:** GO:0035694 derives from a broad parent class (Organelle-specific protein degradation), while the Vms-pathway child itself is `no_mapping`; the mapping audit flagged it `manual_gene_level_review_required`. Gene-level review here rejects propagation to ANKZF1 — node mapping should not project GO:0035694 onto ANKZF1.
- **Evidence alignment:** PN row-3 cites PMID:28451587 (VIM); review/DR use PMID:21896481 (Stapf VIM definition) instead — both VIM-defining, complementary. Strong overlap on RQC PMIDs (29632312, 30244831, 31011209, 32075755). MF refined: RNA-endonuclease/GO:0140101 MODIFY→GO:0004549 tRNA-specific ribonuclease (verified real). ERAD IBA (GO:0036503) REMOVEd as Vms1/Cdc48 over-projection.
- **Verdict:** Consistent; GO:0006515 ACCEPT matches PN. PN GO:0035694 over-reaches for ANKZF1; review correctly declines (no human mito-catabolism evidence).
- **Recommended edits:** none to ANKZF1-ai-review.yaml. [MAP] do not propagate GO:0035694 from the mito "Organelle-specific protein degradation" class to ANKZF1 (Vms-pathway leaf is no_mapping; gene-level evidence is RQC/stress-translocation, not mito proteolysis).