## ARL8A

- **UniProt:** Q96BM9 · **batch:** proteostasis-batch-2026-06-03 (Falcon DR 2026-06-07) · **review status:** COMPLETE
- **PN placement:** 2 rows, ALP. (1) `…Localization of the autophagosome|Movement of autophagosomes along microtubules|HOPS-BORC complex bridging`; (2) `…Autophagosome-lysosome docking|HOPS-BORC interaction mediator`. **PN-node mapping:** localization group + movement/bridging leaves=`mapped`→GO:0061906 autophagosome localization (new_to_goa); docking nodes=`context_only`→GO:0061909; class=`context_only`→GO:0016236.
- **Consistency:** Consistent, with a deliberate PN-vs-review divergence (below). DR ↔ notes ↔ YAML agree: ARL8A is a lysosomal/endolysosomal small GTPase for lysosome positioning and BORC-kinesin/dynein-coupled transport; most ARL8A-specific conclusions are paralog-inferred from ARL8B but direct ARL8A contribution shown by ARL8B-KO + ARL8A-siRNA (PMID:27851960).
- **PN story / NEW pressure:** PN's projected term **GO:0061906 autophagosome localization (verified real, new_to_goa): review DECLINES.** Evidence supports lysosome/endolysosome positioning and HOPS-mediated cargo delivery, not direct ARL8A-dependent positioning of autophagosomes as the transported organelle. Over-reaches as written. Review instead anchors on GO:0032418 lysosome localization. Conclude: GO:0061906 not yet supported for ARL8A; recorded as suggested question/experiment.
- **Mapping strategy:** Mapping flagged `manual_gene_level_review_required`; gene-level review here declines projection to ARL8A. The "autophagosome localization" leaf conflates lysosome positioning with autophagosome positioning — for ARL8A the safe shared target is lysosome localization, not autophagosome localization (PN term is narrower-but-mis-targeted: right process family, wrong cargo organelle).
- **Evidence alignment:** PN cites tandfonline/NatComms titles (Arl8 review; BORC; RUFY3/4 = PMID:35314674). Review/notes anchor on PMID:16537643 (ARL8a/b lysosome localization), 25898167 (BORC), 28325809 (PLEKHM1), 35314674 (RUFY3/4). DR added PMID:27851960, 35653304 (cholesterol egress), 37213076 (exosomes, ARL8A/B dKO), 38296963 (DENND6A), 38128568 (BORCS8 disease) statement-only. Strong overlap (RUFY3/4); review broader.
- **Verdict:** Consistent; PN GO:0061906 over-reaches for ARL8A (lysosome vs autophagosome positioning); review correctly declines and uses GO:0032418.
- **Recommended edits:** none to ARL8A-ai-review.yaml. [MAP] do not propagate GO:0061906 to ARL8A from the autophagosome-localization leaf until direct ARL8A-dependent autophagosome positioning is shown; ARL8A's shared target is lysosome localization.