## ATP6AP2
- **UniProt:** O75787 · **batch:** proteostasis-batch-2026-06-03 · **review status:** COMPLETE
- **PN placement:** `ALP|Lysosomal catabolism|Regulation of lysosomal environment|Lysosomal acidification|Regulator of the lysosomal v-ATPase proton pump` (also `...|Pre-initiation autophagy signaling|mTORC1 pathway, upstream|Nutrient sensing|Regulator...`) ; **PN-node mapping:** subtype `mapped`→GO:0060590 ATPase regulator activity (new_to_goa); type `mapped`→GO:0007042 lysosomal lumen acidification (already_in_goa_exact). Both GO terms verified real (OLS).
- **Consistency:** Consistent. Deep research/notes, review YAML, PN annotation, and PN-node mapping all frame ATP6AP2 as an accessory/regulatory V-ATPase subunit driving endolysosomal acidification. Review ADDED GO:0060590 (ATPase regulator activity) and GO:0070072 (V-ATPase complex assembly) as NEW, and ACCEPTs GO:0007042. No contradictions; receptor/Wnt/RAS roles are correctly held KEEP_AS_NON_CORE.
- **PN story / NEW pressure:** PN asserts a lysosomal V-ATPase regulator MF (GO:0060590) not previously in GOA. Review correctly ADDED it (action: NEW, IMP), supported by assembly-factor interaction and acidification-loss phenotypes (PMID:29127204, 30985297, 32276428). GO:0007042 already captured (ACCEPT). Verdict: PN story correctly captured — one ADD (GO:0060590), one already-captured (GO:0007042), no over-reach.
- **Mapping strategy:** Gene supports node mapping. The MF projection (ATPase regulator activity, deliberately broad/safe) is conservative; review even raises whether a narrower "lysosomal V-ATPase accessory" term should exist (suggested_questions). The acidification process projection matches review scope. No TOMM20-style over-broadening.
- **Evidence alignment:** PN cites a single review (V-ATPase in neurodegeneration, biomedcentral). Review draws on primary functional papers (PMID:29127204 glycosylation/autophagy disorder; PMID:30985297 neurodegeneration; PMID:32276428 lysosomal-pH) for the same claims — broader, well-anchored evidence than the PN row, with directional agreement.
- **Verdict:** Consistent; PN regulator-activity projection correctly ADDED (NEW GO:0060590), acidification already captured. No edits required.