## ATP6V0E1
- **UniProt:** O15342 · **batch:** proteostasis-batch-2026-06-06 · **review status:** COMPLETE (thorough; yeast-complementation + cryo-EM grounded)
- **PN placement:** `Autophagy-Lysosome Pathway` two rows — `…|mTORC1 pathway, upstream|Nutrient sensing|V0 lysosomal v-ATPase proton pump component` and `…|Lysosomal catabolism|Regulation of lysosomal environment|Lysosomal acidification|V0 …component` ; **PN-node mapping:** subtype→GO:0046610 (lysosomal V0 domain, mapped/ok); subtype→GO:0033179 (V0 domain, mapped/ok); type→GO:0007042 (lysosomal lumen acidification, mapped/ok).
- **Consistency:** Consistent. Review, notes, and PN all describe e1 as a small dual-TM V0 structural subunit essential for proton-pump function (yeast complementation, PMID:17350184; cryo-EM, PMID:33065002). PN correctly assigns the V0 sector (vs V1 for G1/H). No contradictions.
- **PN story / NEW pressure:** All projected terms VERIFIED real (OLS). GO:0033179 (generic V0 domain) already in GOA/review (ACCEPT). GO:0046610 "lysosomal V0 domain" is a lysosome-specific sibling of the review's GO:0000220 *vacuolar* V0 — re-specification, already captured at function level. GO:0007042 lysosomal lumen acidification is NOT in the review (review has GO:0007035 vacuolar acidification, a broader parent) → defensible ADD as the precise lysosomal BP. Conclude: GO:0007042 = ADD candidate; GO:0046610 already captured by V0-domain terms.
- **Mapping strategy:** Appropriate; subtype correctly leaf-restricted to V0. PN-projected terms are at/below the review's specificity for CC (lysosomal vs vacuolar) and the BP projection is narrower than the review's vacuolar-acidification — no broader-than-review over-reach.
- **Evidence alignment:** Same pattern as the other V-ATPase subunits: PN cites review-article titles not in the review's PMIDs; review is anchored on primary papers (PMID:9556572 original M9.2 characterization, PMID:17350184 e1/e2 complementation, PMID:33065002 cryo-EM). Complementary, no conflict.
- **Verdict:** Consistent. ADD GO:0007042 (lysosomal lumen acidification) as the lysosome-specific refinement of the existing vacuolar-acidification annotation; GO:0046610 already covered by existing V0-domain terms.