## ATP6V1G1
- **UniProt:** O75348 · **batch:** proteostasis-batch-2026-06-06 · **review status:** COMPLETE (thorough; notes + 40+ annotations reviewed)
- **PN placement:** `Autophagy-Lysosome Pathway` two rows — `…|mTORC1 pathway, upstream|Nutrient sensing|V1 lysosomal v-ATPase proton pump component` and `…|Lysosomal catabolism|Regulation of lysosomal environment|Lysosomal acidification|V1 …component` ; **PN-node mapping:** subtype→GO:0046612 (lysosomal V1 domain, mapped/ok); subtype→GO:0033176 (V-ATPase complex, mapped/ok); type→GO:0007042 (lysosomal lumen acidification, mapped/ok); class→GO:0010506 context_only/too_broad.
- **Consistency:** Strong agreement. Deep-research notes, review YAML, and PN annotation all describe G1 as a V1 peripheral-stalk (EG-heterodimer) subunit acidifying lysosomes/endosomes. PN's mTORC1/Ragulator framing matches the review's Reactome mTORC1 context annotations. No contradictions.
- **PN story / NEW pressure:** All three projected terms are VERIFIED real (OLS). GO:0033176 already in GOA/review (ACCEPT). GO:0046612 "lysosomal V1 domain" is a lysosome-specific refinement of the review's GO:0000221 *vacuolar* V1 / GO:0033180 generic V1 (siblings, not strictly subsumed) — defensible but a lateral re-specification, not a missing function. GO:0007042 lysosomal lumen acidification is NOT in this gene's review/GOA (it IS in the ATP6V1H review) → defensible ADD as a downstream BP of the assembled pump. Conclude: GO:0007042 = ADD candidate; GO:0046612 = already captured at function level (vacuolar/generic V1).
- **Mapping strategy:** Node mapping is appropriate; subtype is correctly leaf-restricted to V1. The lysosomal-specific CC terms are narrower than the review's vacuolar terms, so no broader-than-review over-reach (contrast TOMM20/HSPA8 precedent). Scope/status correct.
- **Evidence alignment:** Divergent reference sets — PN cites review-article titles (mTORC1/V-ATPase/neurodegeneration reviews) not present as PMIDs in the review; review is anchored on primary structural/functional papers (PMID:33065002 cryo-EM, PMID:17360703 G/a interaction, PMID:28296633 iron/HIF). Same biology, different (complementary) citations; no conflict.
- **Verdict:** Consistent. ADD GO:0007042 (lysosomal lumen acidification) to bring G1 in line with its paralog ATP6V1H; GO:0046612 already covered by existing V1-domain terms.