## CDK5RAP3
- **UniProt:** Q96JB5 (C53, LZAP, IC53) · **batch:** proteostasis-batch-2026-06-07 · **review status:** COMPLETE (very large annotation set reviewed)
- **PN placement:** THREE rows across TR/ALP/UPS — (1) `Translation|Cytosolic translation|Ribosome-associated QC|UFMylation`; (2) `ALP|Autophagy substrate selection|Selective autophagy receptor|ERphagy`; (3) `UPS|E3 ubiquitin and UBL ligases|UBL modifier cofactors|UFMylation cofactor` ; **PN-node mapping:** RQC-UFMylation type → GO:0071569 protein ufmylation; RQC group → GO:0006515 protein QC; ERphagy type → GO:0061709 reticulophagy; UPS UFMylation-cofactor type+group → no_mapping.
- **Consistency:** Excellent multi-branch consistency. Deep-research, review, and all three PN rows converge on CDK5RAP3 as the substrate adaptor of the UFM1 ribosome E3 ligase (UREL: UFL1/DDRGK1/CDK5RAP3) that mono-UFMylates RPL26 on ER 60S ribosomes (ER-RQC, 60S recycling) and drives ER-phagy. Review carries GO:1990756 (adaptor MF, multiple IDA), GO:0071569 (ufmylation), GO:0072344/GO:0032790 (ribosome rescue/disassembly), GO:0140501 (reticulophagy). Older LZAP/C53 NF-kB, ARF/p53, G2/M-checkpoint roles correctly KEEP_AS_NON_CORE. No contradictions across the three PN branches.
- **PN story / NEW pressure:** Mostly already captured, one defensible ADD. (a) GO:0071569 ufmylation, GO:0061709 reticulophagy — both already in review (the review uses GO:0140501 *positive regulation of* reticulophagy; GO:0061709 reticulophagy is the parent process, consistent). (b) The RQC group projects GO:0006515 (protein quality control for misfolded/incompletely synthesized proteins) flagged new_to_goa — this captures the ER-RQC role and is defensible/ADD-able, though the review already encodes the same biology more specifically via GO:0072344 rescue of stalled cytosolic ribosome + GO:0032790 ribosome disassembly, so GO:0006515 would be a broader sibling. No NEW term invention needed; all terms verified pre-existing.
- **Mapping strategy:** Sound. UFMylation type → GO:0071569 and ERphagy type → GO:0061709 are precise. The UPS "UFMylation cofactor" node correctly no_mapping (CDK5RAP3 is a UBL-system adaptor, not a ubiquitin E3 — avoids mis-projecting GO:0061630). The RQC-group GO:0006515 is broader than the review's specific ribosome-rescue terms; acceptable as a group umbrella but flag it as broader-than-review (do not let it displace the specific GO:0072344/GO:0032790).
- **Evidence alignment:** PN cites 36121123 (non-canonical scaffold E3 / UFMylation — in review, core IDA), plus ERphagy review titles (Stephani/C53 reticulophagy = PMID:32851973, in review). Good overlap with review's UREL evidence (36121123, 37595036, 38383785, 38383789, 36543799). PN and review are well-aligned on sources.
- **Verdict:** Consistent across TR/ALP/UPS; mapping sound. GO:0006515 (RQC group) is broader than the review's specific terms but defensible as an umbrella.
- **Recommended edits:** None required; optionally note GO:0006515 is a broader sibling of the review's GO:0072344/GO:0032790 and should not displace them. [MAP]