## CHMP4A
- **UniProt:** Q9BY43 · **batch:** proteostasis-pr-1217 · **review status:** COMPLETE
- **PN placement:** 2 rows, ALP — (1) `Autophagosome closure maturation and lysosome fusion → Sealing of autophagophore membrane → ESCRT-III complex component`; (2) `Microautophagy → General microautophagy machinery → ESCRT-III complex component`. **PN-node mapping:** ESCRT-III leaves=mapped→GO:0000815 (already_in_goa_exact); sealing group=mapped→GO:0000045 autophagosome assembly (more_specific_than_existing_goa); ancestors context_only (GO:0016236, GO:0016237). **Projected:** GO:0000815 (x2), GO:0000045.
- **Consistency:** Consistent. The review ACCEPTs GO:0000815 ESCRT III complex (IBA + NAS) and the autophagy/endolysosomal context cluster (GO:0006914, GO:0097352 autophagosome maturation, GO:0000421 autophagosome membrane, GO:1902774, GO:1904930 amphisome). The review's own caveat — CHMP4A "was [not] directly assayed as the phagophore-sealing effector" (Snf7-1 knockdown causes autophagosome accumulation, PMID:21975012/17984323) — matches PN routing "sealing" to the safe parent GO:0000045 rather than a sealing-specific term. No contradiction.
- **PN story / NEW pressure:** Already captured. Verified via OLS that **no GO term for "autophagosome closure / phagophore sealing" exists** (GO:0061908 phagophore is a CC only; GO:0000045 is the narrowest process). PN's "sealing" projection therefore correctly degrades to GO:0000045 — but the review does **not** list GO:0000045 for CHMP4A, instead carrying GO:0097352 autophagosome maturation (a sibling) + GO:0006914. Both are defensible; GO:0000045 would be the assembly-side framing of the same sealing biology.
  - **Recommended edits:** consider adding `GO:0000045 autophagosome assembly` (action: NEW or note in suggested_questions) to align with the PN "Sealing of autophagophore membrane → autophagosome assembly" projection, OR explicitly note GO:0097352 already covers the closure/maturation step. Low priority — the maturation term already captures the biology; flag for curator.
- **Mapping strategy:** No change needed. GO:0000815 is already_in_goa_exact (safe). GO:0000045 is more_specific_than_existing_goa (good direction, not over-reach). CHMP4A is a clean ESCRT-III member for both the autophagosome-closure and microautophagy leaves; the node mappings are appropriate.
- **Evidence alignment:** Minimal overlap. PN cites one MDPI review "Key Regulators of Autophagosome Closure"; the review uses none of PN's citations, relying on primary ESCRT-III structural/functional literature (PMID:18209100, 19234443, 17984323, 21975012). Convergent on the ESCRT-III-in-autophagosome-closure concept via independent sources.
- **Verdict:** Consistent; PN projections (GO:0000815, GO:0000045) are both ≤ existing or more-specific. Optional edit: reconcile GO:0000045 vs the review's GO:0097352 for the sealing step.