## CLPX
- **UniProt:** O76031 · **batch:** proteostasis-batch-2026-06-07 · **review status:** COMPLETE
- **PN placement:** `Mitochondrial proteostasis|Organelle-specific protein degradation|Matrix protease` ; **PN-node mapping:** `[group] Matrix protease` → `mapped` GO:0005759 mitochondrial matrix (`already_in_goa_exact`); `[class] Organelle-specific protein degradation` → `mapped` GO:0035694 mitochondrial protein catabolic process (`more_specific_than_existing_goa`); branch unmapped.
- **Consistency:** Consistent on the core. Notes, YAML, and PN agree CLPX is the AAA+ unfoldase subunit of the matrix ClpXP protease (matrix localization, protein catabolism). Both projected GO terms (GO:0005759, GO:0035694) are verified real. One nuance: GO:0035694's GO definition is framed around an intramitochondrial lysosome-like degradation organelle, which is a narrower/atypical concept than ClpXP-mediated soluble-matrix proteolysis; the review instead uses GO:0030163 protein catabolic process + GO:0006508 proteolysis (the GOA term is the non-mitochondrial GO:0030163 IDA).
- **PN story / NEW pressure:** PN's degradation story is already well captured (matrix + protein catabolism). The review's distinctive, well-supported addition is the CLPP-independent chaperone role: GO:0006783 heme biosynthetic process via ALAS activation (action NEW, PMID:25957689, PMID:28874591, EPP2 disease). The PN node does NOT surface this heme/ALAS axis — a PN-side gap rather than a review gap.
- **Mapping strategy:** Largely sound, but the `[class]` projection to GO:0035694 is questionable: its GO definition does not match ClpXP matrix proteolysis well, and the existing GOA term is the broader GO:0030163. Prefer mapping the class to GO:0030163 protein catabolic process (or qualify GO:0035694) to avoid asserting a process the term's definition does not support.
- **Evidence alignment:** No PN reference titles were listed for this row, so no title-level overlap to compare; the review is densely cited (PMID:11923310, 16115876, 22710082, 25957689, 28874591). No conflict.
- **Verdict:** Consistent; degradation captured. PN mapping over-specifies the catabolic process (GO:0035694 definitional mismatch) and omits the heme/ALAS chaperone role.

**Recommended edits:** [MAP] Reconsider `[class]` projection GO:0035694 → use GO:0030163 protein catabolic process (matches GOA + ClpXP biology; GO:0035694's definition is lysosome-like-organelle specific). [MAP] Optionally extend the PN node to reflect CLPX's GO:0006783 heme biosynthesis chaperone role.