## CNOT4
- **UniProt:** O95628 · **batch:** proteostasis-batch-2026-06-07 · **review status:** COMPLETE
- **PN placement:** `Translation|Cytosolic translation|Ribosome-associated QC|Ubiquitination` and `UPS|E3 ubiquitin and UBL ligases|RING|other|C3H1-type ZnF` ; **PN-node mapping:** RQC group mapped→GO:0006515 (protein QC, new_to_goa); Ubiquitination type→GO:0016567 (already_in_goa); RING group→GO:0061630 (already_in_goa). Subtype/branch = no_mapping.
- **Consistency:** Strong. Deep-research notes, review YAML, PN annotation and node mapping all agree: CNOT4 is a C4C4 RING E3 (GO:0061630, multiple EXP) acting in co-translational/RQC contexts (ABCE1 K48-Ub → mitophagy; methylated RBM15). PN's RQC + RING-E3 dual placement matches the review's core_functions. No contradictions.
- **PN story / NEW pressure:** PN's RQC framing projects GO:0006515 "protein quality control for misfolded or incompletely synthesized proteins" (verified real via OLS), which is genuinely **new_to_goa** — confirmed absent from CNOT4-goa.tsv. The review captures ABCE1 ubiquitination/mitophagy and notes the yeast/Drosophila ribosome-associated nascent-chain Ub role as a suggested_question, but does NOT propose a process term for it. GO:0006515 is a defensible ADD for the human RQC role (well-grounded in PMID:29861391). GO:0016567/GO:0061630 already captured.
- **Mapping strategy:** No change to node mapping warranted. RING-group→GO:0061630 and Ubiquitination-type→GO:0016567 are exact and already annotated; class/branch correctly held context_only/too_broad (avoids over-propagating across the whole E3/translation taxonomy). RQC→GO:0006515 is appropriately specific (not over-broad like the rejected TOMM20/HSPA8 precedents).
- **Evidence alignment:** PN row 2 cites the Deshaies/Joazeiro RING-E3 review (PMID:19489725, family-level, verified via PubMed) — not in the CNOT4 review references (review uses gene-specific PMID:11823428/15001359/26575292/29861391). Divergence is benign: PN cites a generic family review; review cites direct CNOT4 biochemistry. No conflict.
- **Verdict:** CONSISTENT — defensible ADD of GO:0006515 (protein QC) to capture the RQC role. **Recommended edits:** [YAML] consider adding GO:0006515 (involved_in, from PMID:29861391 ABCE1/co-translational QC) as a proposed_new_term or NEW annotation to align with the PN RQC projection.