## CWC27
- **UniProt:** Q6UX04 · **batch:** proteostasis-batch-2026-06-07 · **review status:** COMPLETE
- **PN placement:** `Cytonuclear proteostasis | Folding enzyme | Peptidyl-prolyl isomerases | Cyclophilin type` ; **PN-node mapping:** group AND type both mapped, scope=ok_for_propagation_to_go, GO:0003755 peptidyl-prolyl cis-trans isomerase activity (class/branch = no_mapping)
- **Consistency:** CONTRADICTION. PN classifies CWC27 as an active cyclophilin-type peptidyl-prolyl isomerase and propagates GO:0003755 PPIase activity. But the review, UniProt CAUTION, and the GOA all establish CWC27 is a catalytically DEAD pseudo-PPIase: GOA carries GO:0003755 as **NOT|enables** (IDA, PMID:20676357 — no isomerase activity, no cyclosporin binding, Glu at a conserved active-site position). The PN-projected positive GO:0003755 directly contradicts the curated negated annotation.
- **PN story / NEW pressure:** PN asserts PPIase catalysis that the gene-level evidence refutes. GO:0003755 is a real term (it is in the GOA — negated). This is not an ADD candidate; it is a **family-membership over-reach** (IPR cyclophilin signature ignores loss of catalytic residues). The review's true core is structural/scaffold spliceosome membership (GO:0000398 mRNA splicing, GO:0071005 U2-type precatalytic spliceosome, GO:0005681) — a different branch entirely from "folding enzyme."
- **Mapping strategy:** The Peptidyl-prolyl-isomerase group/type → GO:0003755 mapping is reasonable for active cyclophilins generally, but CWC27 is a pseudoenzyme exception and must NOT inherit the positive GO:0003755. Propagating it here would create a false-positive that conflicts with an existing NOT annotation — a worse outcome than the TOMM20/HSPA8 "too broad" cases. Recommend excluding CWC27 from this node's GO:0003755 propagation.
- **Evidence alignment:** PN row references "17588513 rev"-style entries are absent for CWC27 (dossier shows no titles). Review evidence (PMID:20676357 dead-PPIase; PMID:29360106, PMID:33509932, PMID:39068178 spliceosome cryo-EM; PMID:28285769 disease) is all spliceosome/pseudoenzyme. The PPIase-activity claim has no supporting paper for this gene; the only relevant paper (PMID:20676357) refutes it.
- **Verdict:** Inconsistent — PN propagates positive GO:0003755 PPIase activity onto a curated dead pseudo-enzyme that GOA annotates as NOT|GO:0003755. **Recommended edits:** [MAP] exclude CWC27 from the cyclophilin-type GO:0003755 propagation (CWC27 is a catalytically inactive pseudo-PPIase; GOA = NOT|GO:0003755, PMID:20676357); represent CWC27 via its scaffold/spliceosome role instead.