## DNAJC16
- **UniProt:** Q9Y2G8 (ERdj8) · **batch:** proteostasis-batch-2026-06-07b · **review status:** COMPLETE
- **PN placement:** `Cytonuclear proteostasis | Chaperone | HSP70 system | J-domain containing HSP70 cochaperone` (branch CY) ; **PN-node mapping:** type=mapped, scope=ok_for_propagation_to_go, GO:0030544 Hsp70 protein binding (goa_status=new_to_goa)
- **Consistency:** Partial mismatch. Deep-research notes and review agree the gene is poorly characterized (Pharos Tdark, PAN-GO 0): the ONLY experimental function is ER-membrane localization (GO:0005789) and regulation of autophagosome size (GO:0016243, IMP, PMID:32492081). Crucially the review states the "direct molecular activity (presumed J-domain co-chaperone... ) has not been biochemically defined" — so the PN assertion of Hsp70 binding is an inference, not established. The PN node nonetheless tags it as a confident new-to-GOA propagation, which over-states the evidence.
- **PN story / NEW pressure:** PN proposes ADD of GO:0030544 Hsp70 protein binding as new_to_goa. GO:0030544 is verified real (OLS), but for DNAJC16 there is no experimental HSP70-binding/co-chaperone data; the J domain is required for autophagosome enlargement but no HSP70 partner has been identified (the review's own suggested experiment is to test this). This is over-reach at the gene level: the projection rests purely on family/domain membership.
- **Mapping strategy:** The node-level mapping (J-domain cochaperones bind Hsp70) is defensible as a family heuristic, but for DNAJC16 specifically the new_to_goa propagation should be down-weighted to an inference (ISS/family-level) rather than asserted as a confident annotation, since the molecular function is explicitly undefined. PN-projected term is broader than the review's actual core (autophagosome-size regulation), which the node does not capture at all.
- **Evidence alignment:** Single shared paper PMID:32492081 (Yamamoto 2020). PN node carries no DNAJC16-specific reference; the projection is purely taxonomic. No evidence divergence, but no positive evidence for the Hsp70-binding claim either.
- **Verdict:** Over-reaches for this gene — Hsp70 binding is domain-inferred only, no HSP70 partner shown; flag as family-level inference, not new_to_goa. **Recommended edits:** [MAP] downgrade DNAJC16's GO:0030544 projection from new_to_goa confident annotation to family/ISS-level inference (no experimental HSP70 binding); note the established function (autophagosome-size regulation) lies outside this HSP70-cochaperone node.