## DNAJC21
- **UniProt:** Q5F1R6 (DNAJA5) · **batch:** proteostasis-batch-2026-06-07b · **review status:** COMPLETE
- **PN placement:** Two rows — (1) `Cytonuclear proteostasis | Chaperone | HSP70 system | J-domain containing HSP70 cochaperone` (CY); (2) `Translation | Cytosolic translation | Ribosome biogenesis factor | pre-60S complex | non 5S RNP complex` (TR) ; **PN-node mapping:** row1 type=mapped GO:0030544 Hsp70 protein binding (more_specific_than_existing_goa); row2 type(pre-60S)=mapped GO:0030687 preribosome large subunit precursor (new_to_goa), group(ribosome biogenesis factor)=mapped GO:0042254 ribosome biogenesis (new_to_goa); subtype/class/branch no_mapping or context_only.
- **Consistency:** Strong agreement, and the two-row PN placement is the right call. Notes, review and PN all describe a J-domain co-chaperone of HSPA8 that drives 60S ribosomal-subunit maturation (PMID:27346687), binds precursor 45S rRNA, and causes ribosomopathy BMFS3. No contradictions. PN's dual chaperone+ribosome-biogenesis framing matches the review's two core functions (chaperone binding; RNA binding) better than a single HSP70-cochaperone row would.
- **PN story / NEW pressure:** PN proposes three terms, all verified real (OLS): GO:0030544 Hsp70 protein binding, GO:0042254 ribosome biogenesis, GO:0030687 preribosome large subunit precursor. GOA confirms ribosome biogenesis and pre-60S are genuinely ABSENT from existing annotations (goa.tsv has only RNA binding, nucleolus, ribosome NAS, protein folding NAS). Both GO:0042254 and GO:0030687 are well-supported ADDs (PMID:27346687 establishes the conserved 60S-maturation role). GO:0030544: HSPA8 binding is documented (review uses the parent GO:0051087 protein-folding chaperone binding as core MF); GO:0030544 is the verified more-specific child, so here the "more_specific" tag IS accurate.
- **Mapping strategy:** This gene materially improves the node coverage by adding the ribosome-biogenesis arm. status/scope are appropriate (pre-60S → preribosome large-subunit precursor is the direct complex target; group → ribosome biogenesis is the safe process target; branch/class kept context_only as too broad — consistent with the TOMM20/HSPA8/RAB7A "reject broader" precedent). PN-projected terms are appropriately specific, not over-broad.
- **Evidence alignment:** Excellent. Both rest on PMID:27346687 (Tummala 2016, VERIFIED) for 60S maturation, plus PMID:22658674 (RNA-interactome) for RNA binding. PN row-2 reference titles align with the ribosome-biogenesis story.
- **Verdict:** Consistent and exemplary; the two ribosome-biogenesis ADDs (GO:0042254, GO:0030687) are well-supported and genuinely new to GOA, and GO:0030544 is correctly the more-specific HSP70-binding child. No edits required.