## DNAJC28
- **UniProt:** Q9NX36 (C21orf55/C21orf78) · **batch:** proteostasis-batch-2026-06-07b · **review status:** COMPLETE
- **PN placement:** `ER proteostasis|Chaperone|HSP70 system|J-domain containing HSP70 cochaperone` ; **PN-node mapping:** type=mapped, scope=ok_for_propagation_to_go, GO:0030544 Hsp70 protein binding (projected more_specific_than_existing_goa); group/class/branch=no_mapping.
- **Consistency:** COMPARTMENT MISMATCH. Deep research and review YAML agree DNAJC28 is an essentially uncharacterized J-domain protein whose N-terminus resembles a MITOCHONDRIAL targeting presequence (review/notes predict mitochondrial localization; review core MF GO:0051087 protein-folding chaperone binding). The PN row places it in the **ER proteostasis** branch, which conflicts with the review's mitochondrial prediction. Neither localization is experimentally confirmed, so this is an unresolved discrepancy rather than a proven error, but the branch assignment and the review point in different directions.
- **PN story / NEW pressure:** PN asserts GO:0030544 Hsp70 protein binding (verified real). GOA has only GO:0005515 protein binding (one IPI) + GO:0001659 (IBA, marked over-annotated), so "more_specific_than_existing_goa" is loose; effectively new_to_goa. GO:0030544 is a child of the review's GO:0051087, i.e. a narrower, defensible domain-level ADD — but purely predicted.
- **Mapping strategy:** Node status unchanged. Two issues: (1) goa_status should be new_to_goa; (2) the ER-branch placement is questionable given the mitochondrial-presequence prediction — flag for reconciliation. Term direction (narrower than review) is appropriate.
- **Evidence alignment:** PN row no titles; review's sole literature ref is PMID:24407287 (PML/ASC inflammasome — source of the single IPI only, relevance LOW, VERIFIED). No experimental HSP70-binding evidence on either side.
- **Verdict:** Consistent on function (uncharacterized J-protein); GO:0030544 defensible inferred ADD. **Recommended edits:** [MAP] change goa_status to new_to_goa; [MAP] reconcile ER vs mitochondrial branch placement (review predicts mitochondrial targeting).