## GCN1
- **UniProt:** Q92616 · **batch:** proteostasis-batch-2026-06-07c · **review status:** COMPLETE
- **PN placement (4 rows):** `PN regulation|Translation regulator|Integrated stress response|EIF2AK4 modulator`; `Translation|...|Ribosome-associated QC|other RQC processes`; `Translation|...|Ribosome-associated QC|ubiquitination of eEF1A on stalled ribosomes`; `Autophagy-Lysosome Pathway|Autophagy gene expression|ATF4 transcriptional program|Nutrient sensing` ; **PN-node mapping:** ISR group=mapped→GO:0140467; Translation-regulator class=mapped→GO:0006417; RQC group=mapped→GO:0006515; eEF1A-ubiq type=mapped→GO:0016567 (protein ubiquitination); ALP class context_only (GO:0010506); several types/branches no_mapping.
- **Consistency:** Strong and well-aligned. Deep research, notes, and review all describe GCN1 as the ribosome collision/stall sensor with two roles: (1) activating GCN2/EIF2AK4 → eIF2alpha-P → ISR/amino-acid-starvation response, and (2) acting as the collision sensor of the RNF14-RNF25 RQC pathway that degrades A-site-blocking factors (eEF1A, eRF1). Review ACCEPTs GO:0034198 (aa starvation), GO:0140469 (GCN2-mediated signaling), GO:0072344 (rescue of stalled ribosome ×4), GO:0170011 (stalled ribosome sensor ×4), GO:0043539 (kinase activator), GO:0060090 (adaptor), GO:0160127 (protein-RNA crosslink repair). All four PN rows map onto biology the review independently asserts. No contradictions.
- **PN story / NEW pressure:** PN's projected terms — GO:0006417 (regulation of translation, already_in_goa, KEEP_AS_NON_CORE in review), GO:0140467 (ISR signaling, entailed by closure; review uses the child GO:0140469 GCN2-mediated signaling), GO:0006515 (RQC umbrella, verified real), GO:0016567 (protein ubiquitination) — are all real and biology-consistent. GO:0016567 is the one term GCN1's review does NOT carry: GCN1 enables/promotes eEF1A ubiquitination by recruiting RNF14, but GCN1 is the sensor/scaffold, not itself a ubiquitin transferase, so a direct GO:0016567 on GCN1 over-reaches (the ubiquitination is RNF14's catalytic act; GCN1 is involved_in via GO:0072344/GO:0060090). **Mostly already captured**; GO:0016567 over-reaches for the sensor.
- **Mapping strategy:** ISR group→GO:0140467 is broader than the review's GO:0140469 (a child) but defensible as the umbrella; not a TOMM20-style rejection. RQC group→GO:0006515 is fine (umbrella over GO:0072344). The eEF1A-ubiquitination type→GO:0016567 should be treated as a process GCN1 enables indirectly, not a direct MF/BP to project as GCN1's own activity.
- **Evidence alignment:** PN cites one title — "Ribosome binding protein GCN1 regulates the cell cycle and cell proliferation and is essential for the embryonic development of mice (PLOS Genetics)" — which is NOT among the review's references (the review uses 9234705, 36638793, 37651229, 37951215/6 for the sensor/RQC/ISR roles). Divergence: PN's cited paper covers a developmental/proliferation phenotype not used by the review; the mechanistic ISR/RQC evidence base differs.
- **Verdict:** Highly consistent on core sensor/ISR/RQC biology; PN story already captured (review is more granular: GO:0140469, GO:0072344, GO:0170011). One over-reach. **Recommended edits:** [MAP] qualify the eEF1A-ubiquitination type→GO:0016567 projection — GCN1 promotes (does not catalyze) eEF1A ubiquitination; project as involved_in RQC (GO:0072344) rather than as GCN1's own protein-ubiquitination activity. [REF] consider adding the PN-cited GCN1 developmental PLOS Genetics paper to the review references (currently absent) or noting its relevance is non-core.