## GTPBP1
- **UniProt:** O00178 · **batch:** proteostasis-batch-2026-06-07c · **review status:** COMPLETE
- **PN placement:** `Translation|Cytosolic translation|Ribosome-associated QC|Ribosomal rescue` ; **PN-node mapping:** rescue type=mapped→GO:0072344 (rescue of stalled cytosolic ribosome); RQC group=mapped→GO:0006515; class/branch context_only (GO:0002181/GO:0006412 too_broad).
- **Consistency:** Largely consistent on biology, with one term-level mismatch. Deep research, notes, and review agree GTPBP1 is an eEF1A-like translational GTPase that delivers aa-tRNA to the A site but, with non-canonical kinetics (delayed peptide-bond formation), retains aa-tRNA, stalls the ribosome, and promotes exosome-mediated degradation of faulty mRNAs — i.e., couples elongation to mRNA surveillance/RQC (PMID:30108131). Review ACCEPTs GO:0003746 (elongation factor activity), GO:0003924 (GTPase), GO:0071025 (RNA surveillance, IDA), GO:0061014 (positive regulation of mRNA catabolic process). The review does NOT carry GO:0072344 — it frames GTPBP1's QC role as mRNA surveillance/decay (GO:0071025/GO:0061014), not ribosome rescue.
- **PN story / NEW pressure:** PN's "Ribosomal rescue" type→GO:0072344 (verified real; goa_status=more_specific_than_existing_goa) asserts GTPBP1 rescues stalled cytosolic ribosomes. This is the divergence: GTPBP1's documented role is to CAUSE stalling and recruit the exosome to degrade the mRNA (a surveillance/decay outcome), not to split/rescue the stalled ribosome (the PELO/HBS1L/ABCE1 sense of GO:0072344). GTPBP1 GOA contains GO:0071025 but NOT GO:0072344. So GO:0072344 is a candidate NEW term that **over-reaches**: the evidence supports mRNA surveillance (GO:0071025, already present) rather than ribosome rescue. The PN "more_specific_than_existing_goa" framing is questionable — GO:0072344 is not strictly more specific than GO:0071025; they are different processes.
- **Mapping strategy:** This gene's placement under "Ribosomal rescue" is the issue. GTPBP1 fits "other RQC processes" / mRNA surveillance better than "Ribosomal rescue." The rescue type→GO:0072344 projection should not be applied to O00178. RQC group→GO:0006515 (umbrella) is acceptable. Recommend reclassifying or, at minimum, not projecting GO:0072344.
- **Evidence alignment:** PN dossier lists no reference titles for GTPBP1; the single mechanistic anchor (PMID:30108131) is shared with the review (cited HIGH/VERIFIED). The review reads that paper as surveillance/exosomal decay (GO:0071025/GO:0061014); PN reads it as ribosome rescue (GO:0072344) — same paper, divergent term interpretation.
- **Verdict:** Biology consistent; PN's GO:0072344 "Ribosomal rescue" mapping over-reaches vs the review's surveillance/decay framing (GO:0071025 present in GOA; GO:0072344 absent). **Recommended edits:** [MAP] do not project GO:0072344 (rescue of stalled cytosolic ribosome) onto GTPBP1 — its evidenced role is mRNA surveillance/exosomal decay (GO:0071025/GO:0061014), not ribosome rescue; reclassify the PN row from "Ribosomal rescue" to "other RQC processes" (RQC-group→GO:0006515 still applies). [REF] PMID:30108131 supports surveillance, not rescue — note the interpretive divergence.